Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33822.t1 | KEH43166.1 | 58.6 | 162 | 39 | 8 | 8 | 156 | 16 | 162 | 1.40E-22 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33822.t1 | A0A072VNE8 | 58.6 | 162 | 39 | 8 | 8 | 156 | 16 | 162 | 1.0e-22 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene33822.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33822.t1 | MTR_1g085590 | 55.689 | 167 | 38 | 10 | 7 | 156 | 15 | 162 | 7.99e-41 | 139 |
| MS.gene33822.t1 | MTR_1g063150 | 51.829 | 164 | 49 | 7 | 24 | 171 | 30 | 179 | 1.79e-40 | 135 |
| MS.gene33822.t1 | MTR_1g046810 | 40.000 | 165 | 59 | 10 | 22 | 160 | 82 | 232 | 1.12e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAGCGCCTGGCGTTTCT+TGG | 0.200333 | 4.4:-24173929 | None:intergenic |
| GAAAGAGAAAGAAGGGTATT+TGG | 0.272932 | 4.4:-24173671 | None:intergenic |
| AACCAAGACGAGACACTAAT+TGG | 0.312675 | 4.4:-24174142 | None:intergenic |
| TGAATCACAGCGGCGCTAAA+AGG | 0.334747 | 4.4:+24174058 | MS.gene33822:CDS |
| CTGAGCGCCTGGCGTTTCTT+GGG | 0.363120 | 4.4:-24173928 | None:intergenic |
| TTCATTGATATTAATCATGC+TGG | 0.416806 | 4.4:+24173691 | MS.gene33822:CDS |
| GGGTATTTGGCATTGGTTGT+TGG | 0.420713 | 4.4:-24173658 | None:intergenic |
| CATAATCATAATGATGTTGT+TGG | 0.436940 | 4.4:+24173757 | MS.gene33822:CDS |
| AAGCATTGAATGAACATAAG+AGG | 0.452841 | 4.4:+24173833 | MS.gene33822:CDS |
| CTGGAAAACAAAAGATTACA+TGG | 0.458402 | 4.4:+24173710 | MS.gene33822:CDS |
| GAAAGAAGGGTATTTGGCAT+TGG | 0.461253 | 4.4:-24173665 | None:intergenic |
| GCCTGGCGTTTCTTGGGTGG+TGG | 0.482596 | 4.4:-24173922 | None:intergenic |
| CTCTGTTGCATATATCTCGC+GGG | 0.495665 | 4.4:+24174004 | MS.gene33822:CDS |
| TATCAATGAAAGAGAAAGAA+GGG | 0.506201 | 4.4:-24173678 | None:intergenic |
| GCTGTGATTCACATCGATAT+TGG | 0.509973 | 4.4:-24174046 | None:intergenic |
| CTCATGTGATGTTTGCAACA+AGG | 0.514548 | 4.4:+24173795 | MS.gene33822:CDS |
| AGCGCCTGGCGTTTCTTGGG+TGG | 0.515219 | 4.4:-24173925 | None:intergenic |
| ATATCAATGAAAGAGAAAGA+AGG | 0.543310 | 4.4:-24173679 | None:intergenic |
| TCATATTCAAAATCATCACT+TGG | 0.548363 | 4.4:-24174117 | None:intergenic |
| AAGAGGTGTCACTCTCAAAA+CGG | 0.554314 | 4.4:+24173850 | MS.gene33822:CDS |
| TCGTTCAAATCCATATTGCT+TGG | 0.555538 | 4.4:-24174090 | None:intergenic |
| TGGTGGAAACTGATCATCAA+TGG | 0.563165 | 4.4:-24173905 | None:intergenic |
| GTGATTCACATCGATATTGG+CGG | 0.581021 | 4.4:-24174043 | None:intergenic |
| TCCACCACCCAAGAAACGCC+AGG | 0.581476 | 4.4:+24173921 | MS.gene33822:CDS |
| ATGGAAGATGGTGCTGCAGT+TGG | 0.597422 | 4.4:-24173886 | None:intergenic |
| AACTGATCATCAATGGAAGA+TGG | 0.600593 | 4.4:-24173898 | None:intergenic |
| TCTGTTGCATATATCTCGCG+GGG | 0.628828 | 4.4:+24174005 | MS.gene33822:CDS |
| ACTCTGTTGCATATATCTCG+CGG | 0.638755 | 4.4:+24174003 | MS.gene33822:CDS |
| GATATCGATGATGATGATGA+AGG | 0.643057 | 4.4:+24173967 | MS.gene33822:CDS |
| ACATCATTATGATTATGAGT+AGG | 0.652097 | 4.4:-24173751 | None:intergenic |
| CTGTTGCATATATCTCGCGG+GGG | 0.734296 | 4.4:+24174006 | MS.gene33822:CDS |
| AATATCGATGTGAATCACAG+CGG | 0.785372 | 4.4:+24174048 | MS.gene33822:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATCATTATGATTATGAGT+AGG | - | chr4.4:24173754-24173773 | None:intergenic | 25.0% |
| ! | ATATCAATGAAAGAGAAAGA+AGG | - | chr4.4:24173682-24173701 | None:intergenic | 25.0% |
| ! | CATAATCATAATGATGTTGT+TGG | + | chr4.4:24173757-24173776 | MS.gene33822:CDS | 25.0% |
| ! | TATCAATGAAAGAGAAAGAA+GGG | - | chr4.4:24173681-24173700 | None:intergenic | 25.0% |
| ! | TCATATTCAAAATCATCACT+TGG | - | chr4.4:24174120-24174139 | None:intergenic | 25.0% |
| ! | TTCATTGATATTAATCATGC+TGG | + | chr4.4:24173691-24173710 | MS.gene33822:CDS | 25.0% |
| CTGGAAAACAAAAGATTACA+TGG | + | chr4.4:24173710-24173729 | MS.gene33822:CDS | 30.0% | |
| GCAAAAAATTCCAAGCAATA+TGG | + | chr4.4:24174080-24174099 | MS.gene33822:CDS | 30.0% | |
| GCTCAGAAGAAAAAGATATA+TGG | + | chr4.4:24173945-24173964 | MS.gene33822:CDS | 30.0% | |
| !! | AAGCATTGAATGAACATAAG+AGG | + | chr4.4:24173833-24173852 | MS.gene33822:CDS | 30.0% |
| AACTGATCATCAATGGAAGA+TGG | - | chr4.4:24173901-24173920 | None:intergenic | 35.0% | |
| AATATCGATGTGAATCACAG+CGG | + | chr4.4:24174048-24174067 | MS.gene33822:CDS | 35.0% | |
| TCGTTCAAATCCATATTGCT+TGG | - | chr4.4:24174093-24174112 | None:intergenic | 35.0% | |
| ! | GATATCGATGATGATGATGA+AGG | + | chr4.4:24173967-24173986 | MS.gene33822:CDS | 35.0% |
| !! | GAAAGAGAAAGAAGGGTATT+TGG | - | chr4.4:24173674-24173693 | None:intergenic | 35.0% |
| ACTCTGTTGCATATATCTCG+CGG | + | chr4.4:24174003-24174022 | MS.gene33822:CDS | 40.0% | |
| CTCATGTGATGTTTGCAACA+AGG | + | chr4.4:24173795-24173814 | MS.gene33822:CDS | 40.0% | |
| GCTGTGATTCACATCGATAT+TGG | - | chr4.4:24174049-24174068 | None:intergenic | 40.0% | |
| GTGATTCACATCGATATTGG+CGG | - | chr4.4:24174046-24174065 | None:intergenic | 40.0% | |
| TGGTGGAAACTGATCATCAA+TGG | - | chr4.4:24173908-24173927 | None:intergenic | 40.0% | |
| !! | AAGAGGTGTCACTCTCAAAA+CGG | + | chr4.4:24173850-24173869 | MS.gene33822:CDS | 40.0% |
| !! | GAAAGAAGGGTATTTGGCAT+TGG | - | chr4.4:24173668-24173687 | None:intergenic | 40.0% |
| CTCTGTTGCATATATCTCGC+GGG | + | chr4.4:24174004-24174023 | MS.gene33822:CDS | 45.0% | |
| TCTGTTGCATATATCTCGCG+GGG | + | chr4.4:24174005-24174024 | MS.gene33822:CDS | 45.0% | |
| ! | ATCTTTTTCTTCTGAGCGCC+TGG | - | chr4.4:24173942-24173961 | None:intergenic | 45.0% |
| !! | GGGTATTTGGCATTGGTTGT+TGG | - | chr4.4:24173661-24173680 | None:intergenic | 45.0% |
| ATATCTCGCGGGGGTTATGA+AGG | + | chr4.4:24174015-24174034 | MS.gene33822:CDS | 50.0% | |
| CTGTTGCATATATCTCGCGG+GGG | + | chr4.4:24174006-24174025 | MS.gene33822:CDS | 50.0% | |
| TGAATCACAGCGGCGCTAAA+AGG | + | chr4.4:24174058-24174077 | MS.gene33822:CDS | 50.0% | |
| !! | ATGGAAGATGGTGCTGCAGT+TGG | - | chr4.4:24173889-24173908 | None:intergenic | 50.0% |
| CTGAGCGCCTGGCGTTTCTT+GGG | - | chr4.4:24173931-24173950 | None:intergenic | 60.0% | |
| TCCACCACCCAAGAAACGCC+AGG | + | chr4.4:24173921-24173940 | MS.gene33822:CDS | 60.0% | |
| TCTGAGCGCCTGGCGTTTCT+TGG | - | chr4.4:24173932-24173951 | None:intergenic | 60.0% | |
| AGCGCCTGGCGTTTCTTGGG+TGG | - | chr4.4:24173928-24173947 | None:intergenic | 65.0% | |
| GCCTGGCGTTTCTTGGGTGG+TGG | - | chr4.4:24173925-24173944 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 24173634 | 24174149 | 24173634 | ID=MS.gene33822 |
| chr4.4 | mRNA | 24173634 | 24174149 | 24173634 | ID=MS.gene33822.t1;Parent=MS.gene33822 |
| chr4.4 | exon | 24173634 | 24174149 | 24173634 | ID=MS.gene33822.t1.exon1;Parent=MS.gene33822.t1 |
| chr4.4 | CDS | 24173634 | 24174149 | 24173634 | ID=cds.MS.gene33822.t1;Parent=MS.gene33822.t1 |
| Gene Sequence |
| Protein sequence |
