Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33975.t1 | XP_003591993.1 | 98.9 | 461 | 4 | 1 | 1 | 460 | 1 | 461 | 8.30E-256 | 892.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33975.t1 | G7L166 | 32.7 | 413 | 226 | 13 | 69 | 458 | 290 | 673 | 2.0e-48 | 194.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33975.t1 | G7IAF8 | 98.9 | 461 | 4 | 1 | 1 | 460 | 1 | 461 | 6.0e-256 | 892.5 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33975.t1 | MTR_1g096030 | 98.915 | 461 | 4 | 1 | 1 | 460 | 1 | 461 | 0.0 | 936 |
| MS.gene33975.t1 | MTR_7g027190 | 33.411 | 431 | 231 | 15 | 55 | 458 | 272 | 673 | 6.41e-64 | 219 |
| MS.gene33975.t1 | MTR_7g074650 | 32.718 | 379 | 222 | 10 | 84 | 458 | 443 | 792 | 3.40e-52 | 188 |
| MS.gene33975.t1 | MTR_4g102790 | 32.426 | 404 | 232 | 13 | 73 | 460 | 286 | 664 | 1.40e-51 | 185 |
| MS.gene33975.t1 | MTR_3g065980 | 31.463 | 410 | 222 | 15 | 73 | 458 | 166 | 540 | 1.15e-50 | 180 |
| MS.gene33975.t1 | MTR_5g097480 | 31.910 | 398 | 229 | 12 | 76 | 458 | 174 | 544 | 2.35e-48 | 174 |
| MS.gene33975.t1 | MTR_3g089055 | 29.219 | 397 | 237 | 12 | 78 | 458 | 197 | 565 | 6.49e-46 | 167 |
| MS.gene33975.t1 | MTR_3g089055 | 29.219 | 397 | 237 | 12 | 78 | 458 | 202 | 570 | 7.63e-46 | 167 |
| MS.gene33975.t1 | MTR_1g029420 | 29.975 | 397 | 234 | 12 | 78 | 458 | 224 | 592 | 9.29e-46 | 167 |
| MS.gene33975.t1 | MTR_5g094450 | 29.064 | 406 | 234 | 14 | 76 | 458 | 158 | 532 | 5.57e-45 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33975.t1 | AT3G54220 | 34.884 | 387 | 204 | 12 | 84 | 457 | 298 | 649 | 5.92e-55 | 194 |
| MS.gene33975.t1 | AT1G14920 | 33.085 | 402 | 217 | 16 | 72 | 458 | 165 | 529 | 9.07e-54 | 188 |
| MS.gene33975.t1 | AT2G04890 | 32.905 | 389 | 224 | 12 | 79 | 458 | 53 | 413 | 1.86e-52 | 182 |
| MS.gene33975.t1 | AT3G03450 | 32.446 | 413 | 228 | 17 | 64 | 458 | 166 | 545 | 4.38e-51 | 181 |
| MS.gene33975.t1 | AT2G01570 | 30.918 | 414 | 228 | 17 | 64 | 458 | 207 | 581 | 3.72e-50 | 180 |
| MS.gene33975.t1 | AT1G21450 | 31.234 | 397 | 230 | 12 | 78 | 458 | 224 | 593 | 1.36e-49 | 178 |
| MS.gene33975.t1 | AT5G41920 | 31.931 | 404 | 227 | 12 | 69 | 459 | 33 | 401 | 1.40e-49 | 174 |
| MS.gene33975.t1 | AT5G17490 | 32.282 | 412 | 223 | 18 | 63 | 458 | 145 | 516 | 2.09e-49 | 176 |
| MS.gene33975.t1 | AT1G66350 | 30.976 | 410 | 227 | 17 | 64 | 458 | 138 | 506 | 6.56e-48 | 172 |
| MS.gene33975.t1 | AT5G48150 | 30.633 | 395 | 231 | 13 | 80 | 458 | 123 | 490 | 3.92e-47 | 170 |
Find 59 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTAGAATGGAGAATCA+TGG | 0.508498 | 1.4:+74762453 | MS.gene33975:CDS |
| AAATGAGCAAATTGGAAATA+CGG | 0.480919 | 1.4:-74761772 | None:intergenic |
| AACCTTTCAATACTCTCAAG+TGG | 0.550308 | 1.4:-74762321 | None:intergenic |
| AACTATGATGATGTGGATGG+TGG | 0.649454 | 1.4:+74762393 | MS.gene33975:CDS |
| AACTCATATGAAGGCTATGA+AGG | 0.484565 | 1.4:+74761418 | MS.gene33975:CDS |
| AAGAAGGTGGTGGTGGTTTC+AGG | 0.088549 | 1.4:+74762568 | MS.gene33975:CDS |
| AAGGTTAAGAATTGAGAAGA+AGG | 0.444053 | 1.4:+74762338 | MS.gene33975:CDS |
| AATAACTATGATGATGTGGA+TGG | 0.639203 | 1.4:+74762390 | MS.gene33975:CDS |
| AATATGAAAAGATGGAAACA+TGG | 0.637098 | 1.4:+74762430 | MS.gene33975:CDS |
| AATCAATAACATGAATAGAA+CGG | 0.614448 | 1.4:-74761849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGTAGAATGGAGAATCA+TGG | + | chr1.4:74762453-74762472 | MS.gene33975:CDS | 30.0% | |
| AAGGTTAAGAATTGAGAAGA+AGG | + | chr1.4:74762338-74762357 | MS.gene33975:CDS | 30.0% | |
| ACCATTCTATGAAATGTTGA+TGG | + | chr1.4:74761693-74761712 | MS.gene33975:CDS | 30.0% | |
| ACTCTCATTGAAGATTCAAA+TGG | + | chr1.4:74761490-74761509 | MS.gene33975:CDS | 30.0% | |
| AGAATCTCTTTCAGATTAAC+TGG | + | chr1.4:74761943-74761962 | MS.gene33975:CDS | 30.0% | |
| GAAGAAGAAAAATGAAACTG+TGG | + | chr1.4:74762092-74762111 | MS.gene33975:CDS | 30.0% | |
| GTGAGAAGTAAGTGAATTAA+TGG | - | chr1.4:74761520-74761539 | None:intergenic | 30.0% | |
| TCCATCAACATTTCATAGAA+TGG | - | chr1.4:74761697-74761716 | None:intergenic | 30.0% | |
| TTATTGTCCACTTCAATTTG+AGG | + | chr1.4:74762545-74762564 | MS.gene33975:CDS | 30.0% | |
| TTGTTCAACAACAACAACAA+TGG | - | chr1.4:74762205-74762224 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 74761295 | 74762677 | 74761295 | ID=MS.gene33975 |
| chr1.4 | mRNA | 74761295 | 74762677 | 74761295 | ID=MS.gene33975.t1;Parent=MS.gene33975 |
| chr1.4 | exon | 74761295 | 74762677 | 74761295 | ID=MS.gene33975.t1.exon1;Parent=MS.gene33975.t1 |
| chr1.4 | CDS | 74761295 | 74762677 | 74761295 | ID=cds.MS.gene33975.t1;Parent=MS.gene33975.t1 |
| Gene Sequence |
>MS.gene33975 ATGGAAGCTGATTCAGAAGAAGATGAGCTTCTGAATCTTAGCCTTTCAGTTAATAGAGAAAGAAAAAAGAAGGGAAAAATCATCATAAGTAGAGAAAATAACAACATGATGAGTACTAATAGAAACTCATATGAAGGCTATGAAGGAAAAATCTTTCATCTTCTTCAAATGAGAGAACAAATGCTAAGAAAAAGCACTCTCATTGAAGATTCAAATGGTCTTCCATTAATTCACTTACTTCTCACAACAGCAACTTCTGTTGATGAAAACAACTTGGATTCATCTCTTGAAAACCTAACAGATCTTTACCAAACAGTTTCCCTCACCGGTGATTCGGTTCAACGTGTCGTCGCATATTTCACCGATGGTTTAACTGCAAAACTCCTCACAAAAAAATCACCATTCTATGAAATGTTGATGGAAGAACCAACAATCGACGAAGAGTTTCTCGCATTCACAGATCTCTACAGAGTTTCACCGTATTTCCAATTTGCTCATTTCACTGCAAATCAAGCTATCTTGGAAGCCTTTGAAAAAGAAGAAGAGAAAAATAACCGTTCTATTCATGTTATTGATTTTGATGTTTCATATGGTTTTCAATGGCCTTCACTGATTCAATCACTTTCAGAAAAAGCAACAAGTGGCAACAGAATCTCTTTCAGATTAACTGGTTTCGGTAAGAATTTAAAAGAGCTTCAAGAAACTGAATCTAGGTTAGTTAGTTTCTCAAAAGGTTTTGGTAACATTGTTTTTGAGTTTCAAGGTTTGTTAAGAGGATCAAGAGTAATTAATTTGAGGAAGAAGAAAAATGAAACTGTGGCGGTTAATTTAGTTTCCTATTTAAACAAAATGAGTTGTTTATTGAAAATAACTGACACATTGGGATTTGTTCATTCTCTTAACCCTTCCATTGTTGTTGTTGTTGAACAAGAAGGTAGTAAAAATCCTAGTAGAACCTTCTTATCAAGATTCACAGACACATTGCATTATTTTGCAGCTATGTTTGATTCACTTGATGATTGTTTACCACTTGAGAGTATTGAAAGGTTAAGAATTGAGAAGAAGGTTTTTGGTAAAGAGATTAAAAGCATGCTTAATAACTATGATGATGTGGATGGTGGTGTTGATTGTGCAAAATATGAAAAGATGGAAACATGGAAAAGTAGAATGGAGAATCATGGATTTGTTGGTATGAAAATGAGTTCAAAATGTTTAATACAAGCAAAATTGTTGCTTAAGATGAGAACACATTATTGTCCACTTCAATTTGAGGAAGAAGGTGGTGGTGGTTTCAGGGTTTCAGAAAGAGATGATGGAAGGGCTATCTCTTTAGGATGGCAAAATAGGTTTTTGCTTACTGTGTCAGCATGGCAATCACTTTGA |
| Protein sequence |
>MS.gene33975.t1 MEADSEEDELLNLSLSVNRERKKKGKIIISRENNNMMSTNRNSYEGYEGKIFHLLQMREQMLRKSTLIEDSNGLPLIHLLLTTATSVDENNLDSSLENLTDLYQTVSLTGDSVQRVVAYFTDGLTAKLLTKKSPFYEMLMEEPTIDEEFLAFTDLYRVSPYFQFAHFTANQAILEAFEKEEEKNNRSIHVIDFDVSYGFQWPSLIQSLSEKATSGNRISFRLTGFGKNLKELQETESRLVSFSKGFGNIVFEFQGLLRGSRVINLRKKKNETVAVNLVSYLNKMSCLLKITDTLGFVHSLNPSIVVVVEQEGSKNPSRTFLSRFTDTLHYFAAMFDSLDDCLPLESIERLRIEKKVFGKEIKSMLNNYDDVDGGVDCAKYEKMETWKSRMENHGFVGMKMSSKCLIQAKLLLKMRTHYCPLQFEEEGGGGFRVSERDDGRAISLGWQNRFLLTVSAWQSL |
