AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33984.t1 MTR_6g051425 30.182 275 175 6 50 311 19 289 2.99e-35 137
MS.gene33984.t1 MTR_0856s0010 49.612 129 64 1 46 173 32 160 4.44e-33 125
MS.gene33984.t1 MTR_4g035820 30.405 296 180 12 9 288 39 324 8.69e-24 107
MS.gene33984.t1 MTR_0558s0020 25.260 289 182 8 30 284 8 296 4.56e-22 97.8
MS.gene33984.t1 MTR_1g070355 28.936 235 152 7 54 278 80 309 1.33e-20 97.1
MS.gene33984.t1 MTR_7g446060 51.220 82 40 0 130 211 7 88 5.96e-20 87.0
MS.gene33984.t1 MTR_8g446020 24.576 236 157 4 52 284 39 256 2.14e-17 83.2
MS.gene33984.t1 MTR_3g450850 26.829 287 191 8 2 272 3 286 2.18e-17 85.1
MS.gene33984.t1 MTR_7g446480 31.977 172 116 1 122 292 143 314 5.12e-17 83.2
MS.gene33984.t1 MTR_6g022070 28.966 145 99 1 338 482 1 141 7.76e-17 83.2
MS.gene33984.t1 MTR_1g008290 32.331 133 88 1 108 240 14 144 1.03e-16 78.2
MS.gene33984.t1 MTR_7g446510 32.530 166 111 1 128 292 164 329 1.96e-16 81.6
MS.gene33984.t1 MTR_7g446520 25.847 236 162 2 52 286 92 315 2.36e-16 81.6
MS.gene33984.t1 MTR_7g115320 61.111 54 18 1 137 190 9 59 7.94e-15 70.9
MS.gene33984.t1 MTR_1g083640 23.162 272 175 8 18 255 11 282 1.77e-14 75.1
MS.gene33984.t1 MTR_1g019850 32.331 133 79 4 48 175 70 196 7.56e-14 72.4
MS.gene33984.t1 MTR_3g012160 41.975 81 46 1 193 273 14 93 1.36e-13 67.4
MS.gene33984.t1 MTR_5g023310 28.662 157 43 3 179 334 16 104 2.94e-13 67.0
MS.gene33984.t1 MTR_5g076070 23.552 259 185 7 23 272 28 282 4.70e-13 70.9
MS.gene33984.t1 MTR_6g090505 25.843 178 129 2 109 283 53 230 2.24e-12 69.7
MS.gene33984.t1 MTR_0402s0040 25.000 180 130 3 47 221 25 204 4.13e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33984.t1 AT4G15090 32.425 734 455 15 48 771 51 753 2.34e-123 390
MS.gene33984.t1 AT4G15090 32.425 734 455 15 48 771 51 753 2.34e-123 390
MS.gene33984.t1 AT4G15090 32.425 734 455 15 48 771 51 753 2.34e-123 390
MS.gene33984.t1 AT4G15090 32.425 734 455 15 48 771 51 753 2.34e-123 390
MS.gene33984.t1 AT4G15090 32.425 734 455 15 48 771 51 753 2.34e-123 390
MS.gene33984.t1 AT4G38180 32.514 732 441 18 8 705 38 750 3.35e-119 378
MS.gene33984.t1 AT3G22170 29.782 779 471 14 18 770 35 763 3.62e-111 358
MS.gene33984.t1 AT3G22170 29.782 779 471 14 18 770 35 763 3.62e-111 358
MS.gene33984.t1 AT1G76320 32.198 587 374 9 52 633 1 568 5.48e-105 337
MS.gene33984.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.19e-104 338
MS.gene33984.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.54e-104 338
MS.gene33984.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.54e-104 338
MS.gene33984.t1 AT1G52520 33.040 569 360 11 47 607 84 639 6.45e-104 335
MS.gene33984.t1 AT3G06250 33.510 567 317 15 48 606 190 704 1.35e-92 306
MS.gene33984.t1 AT3G06250 33.510 567 317 15 48 606 190 704 1.35e-92 306
MS.gene33984.t1 AT3G06250 33.510 567 317 15 48 606 190 704 1.35e-92 306
MS.gene33984.t1 AT2G27110 31.639 610 364 14 48 645 49 617 1.47e-92 308
MS.gene33984.t1 AT2G27110 31.639 610 364 14 48 645 49 617 1.47e-92 308
MS.gene33984.t1 AT1G80010 32.428 589 354 12 48 610 95 665 2.27e-90 300
MS.gene33984.t1 AT2G27110 34.728 478 289 9 172 645 14 472 7.10e-88 293
MS.gene33984.t1 AT5G18960 31.894 602 355 16 28 622 192 745 3.85e-85 287
MS.gene33984.t1 AT5G18960 31.894 602 355 16 28 622 192 745 3.85e-85 287
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 3.64e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 135 680 4.28e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 135 680 5.34e-81 275
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 6.20e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 6.20e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 6.20e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 6.68e-81 276
MS.gene33984.t1 AT2G32250 30.973 565 353 11 224 770 176 721 6.68e-81 276
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 24 583 7.91e-79 269
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 24 583 7.91e-79 269
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 24 583 8.34e-79 269
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 24 583 1.45e-78 269
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 24 583 1.45e-78 269
MS.gene33984.t1 AT4G19990 30.198 606 353 12 51 632 38 597 1.58e-78 269
MS.gene33984.t1 AT4G38170 33.763 388 246 4 221 606 21 399 4.63e-75 254
MS.gene33984.t1 AT4G19990 30.983 539 318 10 104 632 32 526 1.54e-74 256
MS.gene33984.t1 AT1G10240 26.868 629 409 17 1 603 1 604 1.69e-64 228
MS.gene33984.t1 AT1G10240 26.868 629 409 17 1 603 1 604 1.69e-64 228
MS.gene33984.t1 AT5G28530 27.946 594 364 13 46 603 53 618 1.60e-61 220
MS.gene33984.t1 AT5G28530 27.441 594 363 12 46 603 53 614 1.05e-60 218
MS.gene33984.t1 AT5G28530 27.441 594 363 12 46 603 53 614 1.05e-60 218
MS.gene33984.t1 AT5G28530 26.829 328 202 7 290 603 252 555 1.84e-27 119
MS.gene33984.t1 AT5G28530 28.324 173 107 5 46 205 53 221 8.59e-11 65.9
MS.gene33984.t1 AT5G28530 25.915 328 201 6 290 603 252 551 9.80e-27 116
MS.gene33984.t1 AT5G28530 28.324 173 107 5 46 205 53 221 8.69e-11 65.9

Find 159 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGTTGACGTTTCGGATTC+CGG 0.204382 1.4:-74648168 MS.gene33984:CDS
TCTTTCCCGCTCTTCGAAAA+TGG 0.249066 1.4:+74647006 None:intergenic
TAAGATGACCAAGTACAAAA+AGG 0.265908 1.4:+74646504 None:intergenic
GATTGCAAACCCTTGTTTCA+TGG 0.302647 1.4:+74648041 None:intergenic
AAGACCGCAACCTAGTAAAA+TGG 0.321194 1.4:+74647360 None:intergenic
GTGTTGACGTTTCGGATTCC+GGG 0.344560 1.4:-74648167 MS.gene33984:CDS
GGCTATGCAGAATGCAATTC+AGG 0.347065 1.4:-74647236 MS.gene33984:CDS
TTGCTTTAAAGAAGGGTCTA+TGG 0.361166 1.4:-74647870 MS.gene33984:CDS
TGCGGCTCGTGTTGACGTTT+CGG 0.362249 1.4:-74648175 MS.gene33984:CDS
ATGCCATTTGCCCCGTTTGT+TGG 0.369173 1.4:-74647403 MS.gene33984:CDS
ACTAAATCACGGTTGTTTGC+TGG 0.375082 1.4:-74647802 MS.gene33984:CDS
AGTATTGTGTTGATTTCAAC+GGG 0.389824 1.4:-74646592 MS.gene33984:CDS
CACCACCTATGCCTAGTATT+AGG 0.393202 1.4:+74647205 None:intergenic
GTACACCCATGACAAGTATA+TGG 0.398379 1.4:-74646738 MS.gene33984:CDS
GGATTAAGAATTGGTTGATC+AGG 0.405554 1.4:+74646170 None:intergenic
CTAGGCATAGGTGGTGTCTA+TGG 0.405638 1.4:-74647198 MS.gene33984:CDS
CCACACATTGAAAGATTTGA+AGG 0.409966 1.4:-74646377 MS.gene33984:CDS
ACTTCGACAACCGAAACAAT+TGG 0.413255 1.4:+74646050 None:intergenic
AAAGGATGATCGATCAATAT+AGG 0.415391 1.4:+74648228 None:intergenic
CCTCTGTGTTGTTGACATTC+CGG 0.424793 1.4:+74646949 None:intergenic
GATGATTGACACCAACAAAC+GGG 0.432575 1.4:+74647392 None:intergenic
TTGGTGGTATGTTTGATTCA+TGG 0.433399 1.4:+74647944 None:intergenic
ACACCAACAAACGGGGCAAA+TGG 0.438957 1.4:+74647400 None:intergenic
GCAAACAACCGTGATTTAGT+TGG 0.439208 1.4:+74647805 None:intergenic
ATGGTTTGGGTGAAAATGAT+TGG 0.445440 1.4:-74647036 MS.gene33984:CDS
TGATGATTGACACCAACAAA+CGG 0.449717 1.4:+74647391 None:intergenic
CCATCAGCTAGCTCTCCGTC+AGG 0.451558 1.4:+74648198 None:intergenic
ACACAATACTTAGTTTCGTT+CGG 0.457073 1.4:+74646605 None:intergenic
GGCTAAGGTGTATGTCTTCG+AGG 0.457503 1.4:-74647291 MS.gene33984:CDS
TATCCTACTTTGCTAGAATG+AGG 0.459924 1.4:-74647579 MS.gene33984:CDS
AAAACTAGAAACTCTAAGAA+AGG 0.460138 1.4:-74648018 MS.gene33984:CDS
AAACTAGAAACTCTAAGAAA+GGG 0.460938 1.4:-74648017 MS.gene33984:CDS
ACTCCAAGACAAGGCTGAAA+AGG 0.462412 1.4:-74646843 MS.gene33984:CDS
CAAATGGTCTACCAAGAAGT+TGG 0.463702 1.4:-74646273 MS.gene33984:CDS
AGGTTGTGTTTCCTAATACT+AGG 0.465284 1.4:-74647216 MS.gene33984:CDS
GCAAAAGAAAGACACCTTCT+CGG 0.472868 1.4:-74647628 MS.gene33984:CDS
GATAACGGTTGCTTTAAAGA+AGG 0.473052 1.4:-74647878 MS.gene33984:CDS
AACATCACCAAAGTATTCAT+AGG 0.473828 1.4:+74647465 None:intergenic
TTGAAATTGTCTCTCGATTG+TGG 0.474751 1.4:+74646766 None:intergenic
GTTTCAAGCGTCCGGTGATA+CGG 0.475511 1.4:-74645895 MS.gene33984:CDS
TTTGTTGGTGTCAATCATCA+TGG 0.475674 1.4:-74647388 MS.gene33984:CDS
TCCGATGGGTTTCAAGCGTC+CGG 0.479506 1.4:-74645903 MS.gene33984:CDS
GCCACATTGTGGTGTGGGAG+GGG 0.479684 1.4:-74646234 MS.gene33984:CDS
GAGCAATCAAGTTTCTTCGT+TGG 0.480101 1.4:+74647907 None:intergenic
ATTTGTTGTTTCAAAGGTCC+CGG 0.482096 1.4:+74648149 None:intergenic
CACTTATGCTCGATGGCTGA+TGG 0.482201 1.4:-74648255 None:intergenic
TCGGGAAGGAAGGAGATGGC+AGG 0.482875 1.4:-74647609 MS.gene33984:CDS
CCTTCAAATCTTTCAATGTG+TGG 0.491616 1.4:+74646377 None:intergenic
CTTGTTTCATGGCATATTCA+CGG 0.492232 1.4:+74648052 None:intergenic
AAAACCACTAGTTGACTGTT+GGG 0.492621 1.4:+74645857 None:intergenic
TCCGTTAAAAGTACCACGTA+AGG 0.492742 1.4:-74646150 MS.gene33984:CDS
GACAATACTCTCCTCTAAAA+CGG 0.498094 1.4:+74646637 None:intergenic
CGTGAATATGCCATGAAACA+AGG 0.498225 1.4:-74648051 MS.gene33984:CDS
ACATTACTTCCTATGTCCGA+TGG 0.499257 1.4:-74645918 MS.gene33984:CDS
TGTATGAAGAAATGATCAAA+TGG 0.501577 1.4:-74646289 MS.gene33984:CDS
TTAAAAGACATACCGATAGT+AGG 0.501742 1.4:+74648102 None:intergenic
AAATGGTCTACCAAGAAGTT+GGG 0.502052 1.4:-74646272 MS.gene33984:CDS
TTTCGAAGAGCGGGAAAGAT+GGG 0.502201 1.4:-74647002 MS.gene33984:CDS
GTTGGATTCGTCTTCAAAGT+TGG 0.502236 1.4:+74647925 None:intergenic
ATACTTGTCATGGGTGTACT+CGG 0.502362 1.4:+74646742 None:intergenic
CATCTCCTTCCTTCCCGAGA+AGG 0.502837 1.4:+74647614 None:intergenic
TTACCTGGTTGTTCGAAACA+TGG 0.503284 1.4:-74647312 MS.gene33984:CDS
TGGTCATCTTAGAGTGAAAG+AGG 0.503540 1.4:-74646492 MS.gene33984:CDS
TATGTCTTCGAGGCCTCCAA+TGG 0.508338 1.4:-74647281 MS.gene33984:CDS
GACAACATTTGTTGTTTCAA+AGG 0.508809 1.4:+74648143 None:intergenic
CATTACTTCCTATGTCCGAT+GGG 0.511102 1.4:-74645917 MS.gene33984:CDS
TCGGTTACAATTCCCATTGG+AGG 0.511504 1.4:+74647268 None:intergenic
TTACGAAAATCTAACATTTG+TGG 0.512305 1.4:-74647674 MS.gene33984:CDS
AAACGATCAGGCAGGTGTAC+GGG 0.512319 1.4:-74647737 MS.gene33984:CDS
CGTTCCATGGTATGTGATGC+CGG 0.512631 1.4:-74647700 MS.gene33984:CDS
GAGAGATGTCAGAAACTTTG+TGG 0.514693 1.4:-74647650 MS.gene33984:CDS
TCTGGGCTGATGCACGTAGT+AGG 0.516810 1.4:-74647492 MS.gene33984:CDS
CAAAAGAAAGACACCTTCTC+GGG 0.517286 1.4:-74647627 MS.gene33984:CDS
GTATCTCCATATACTTGTCA+TGG 0.517305 1.4:+74646732 None:intergenic
ATAACGGTTGCTTTAAAGAA+GGG 0.522275 1.4:-74647877 MS.gene33984:CDS
TTCCCTCATTCTAGCAAAGT+AGG 0.524536 1.4:+74647576 None:intergenic
GAACAAAAGTTTCCGTTCCA+TGG 0.529264 1.4:-74647713 MS.gene33984:CDS
GGATTCGTCTTCAAAGTTGG+TGG 0.530111 1.4:+74647928 None:intergenic
TAAACGATCAGGCAGGTGTA+CGG 0.533293 1.4:-74647738 MS.gene33984:CDS
AAGTATTGTGTTGATTTCAA+CGG 0.536214 1.4:-74646593 MS.gene33984:CDS
TAAAACCACTAGTTGACTGT+TGG 0.536849 1.4:+74645856 None:intergenic
ATCCTACTTTGCTAGAATGA+GGG 0.537508 1.4:-74647578 MS.gene33984:CDS
TGATCGGTTACAATTCCCAT+TGG 0.541217 1.4:+74647265 None:intergenic
CATCAGCTAGCTCTCCGTCA+GGG 0.541329 1.4:+74648199 None:intergenic
CTGCATAGCCTTGCACTGAT+CGG 0.542185 1.4:+74647249 None:intergenic
CTATGAGATTGACATGGACG+AGG 0.547341 1.4:-74647536 MS.gene33984:CDS
TGTTTGTTGTTTGAGTTCAG+AGG 0.550114 1.4:-74646545 MS.gene33984:CDS
TGATTGCTCAGCTAAGATAA+CGG 0.550952 1.4:-74647893 MS.gene33984:CDS
GATAAATTCACGCCTACTAT+CGG 0.551302 1.4:-74648114 MS.gene33984:CDS
TATCTCCATATACTTGTCAT+GGG 0.551454 1.4:+74646733 None:intergenic
ATTCTAACTTGTGCAAGAGA+AGG 0.552020 1.4:-74647970 MS.gene33984:CDS
AGGTCTGCCTATGAATACTT+TGG 0.552277 1.4:-74647472 MS.gene33984:CDS
AGACACCTTCTCGGGAAGGA+AGG 0.553011 1.4:-74647619 MS.gene33984:CDS
CAGCCACATTGTGGTGTGGG+AGG 0.557227 1.4:-74646236 MS.gene33984:CDS
TTACAGCCACATTGTGGTGT+GGG 0.557892 1.4:-74646239 MS.gene33984:CDS
TGTTTCGAACAACCAGGTAA+AGG 0.558836 1.4:+74647315 None:intergenic
AACTCTAAGAAAGGGGCCGA+TGG 0.560180 1.4:-74648009 MS.gene33984:CDS
AGCCACATTGTGGTGTGGGA+GGG 0.562336 1.4:-74646235 MS.gene33984:CDS
CTAAATTTATCTTGCCACGA+TGG 0.563368 1.4:-74646463 MS.gene33984:CDS
GAGATACAAGTAGAATTTCG+TGG 0.566723 1.4:-74646716 MS.gene33984:CDS
GCTTGAAACCCATCGGACAT+AGG 0.568910 1.4:+74645909 None:intergenic
ATTGTGGAGTTTGACCCGCA+TGG 0.569243 1.4:+74646782 None:intergenic
GAACAACCAGGTAAAGGACT+TGG 0.570795 1.4:+74647321 None:intergenic
ATGTCTTCGAGGCCTCCAAT+GGG 0.571048 1.4:-74647280 MS.gene33984:CDS
TGTTTCCTAATACTAGGCAT+AGG 0.571109 1.4:-74647210 MS.gene33984:CDS
TTGTTGGTGTCAATCATCAT+GGG 0.576932 1.4:-74647387 MS.gene33984:CDS
TCTCGAAGGTGTGACTACAT+TGG 0.577648 1.4:-74646678 MS.gene33984:CDS
GGATCATAGCTTAAACGCGA+TGG 0.582325 1.4:-74646213 MS.gene33984:CDS
ATCAGCTAGCTCTCCGTCAG+GGG 0.582853 1.4:+74648200 None:intergenic
TTGTGTGTATGATTCTCTGC+GGG 0.589220 1.4:-74647101 MS.gene33984:CDS
CATTTCTTCATACAAATCAG+AGG 0.596809 1.4:+74646298 None:intergenic
GTGAATATGCCATGAAACAA+GGG 0.602322 1.4:-74648050 MS.gene33984:CDS
AAGATACCAAGTCCTTTACC+TGG 0.605756 1.4:-74647327 MS.gene33984:CDS
CGACAACGCACTCCAAGACA+AGG 0.606607 1.4:-74646852 MS.gene33984:CDS
AGATACAAGTAGAATTTCGT+GGG 0.607840 1.4:-74646715 MS.gene33984:CDS
AGAAAGACACCTTCTCGGGA+AGG 0.609091 1.4:-74647623 MS.gene33984:CDS
TCGGACATAGGAAGTAATGT+AGG 0.609568 1.4:+74645921 None:intergenic
GACAACCGACGATTAGTTCG+AGG 0.611005 1.4:+74646122 None:intergenic
TGCATCAATGAGCAAAGATG+TGG 0.611914 1.4:-74645823 MS.gene33984:CDS
ACCACCGGCATCACATACCA+TGG 0.613048 1.4:+74647696 None:intergenic
AAATGCAACTGTTATCTCGA+AGG 0.615193 1.4:-74646692 MS.gene33984:CDS
TTCCTAATACTAGGCATAGG+TGG 0.615209 1.4:-74647207 MS.gene33984:CDS
CAAGCACAATCATTCCATGC+GGG 0.616971 1.4:-74646796 MS.gene33984:CDS
GGTTGTTCGAAACATGGCTA+AGG 0.617470 1.4:-74647306 MS.gene33984:CDS
CGTGGCAAGATAAATTTAGA+CGG 0.619992 1.4:+74646467 None:intergenic
AATTGTAACCGATCAGTGCA+AGG 0.620761 1.4:-74647257 MS.gene33984:CDS
CGGGAAGGAAGGAGATGGCA+GGG 0.622349 1.4:-74647608 MS.gene33984:CDS
GAAGTAGTTAACCGTATCAC+CGG 0.622793 1.4:+74645884 None:intergenic
TCATCCCAACAGTCAACTAG+TGG 0.623204 1.4:-74645861 MS.gene33984:CDS
CCTGACGGAGAGCTAGCTGA+TGG 0.623440 1.4:-74648198 MS.gene33984:CDS
CCGTTAAAAGTACCACGTAA+GGG 0.624486 1.4:-74646149 MS.gene33984:CDS
CGGCATCACATACCATGGAA+CGG 0.625469 1.4:+74647701 None:intergenic
TAGCCATGTTTCGAACAACC+AGG 0.627145 1.4:+74647309 None:intergenic
GTTCGAGGACGTCCCTTACG+TGG 0.628497 1.4:+74646137 None:intergenic
CTTCTCGGGAAGGAAGGAGA+TGG 0.631777 1.4:-74647613 MS.gene33984:CDS
TTTACAGCCACATTGTGGTG+TGG 0.632168 1.4:-74646240 MS.gene33984:CDS
AACTAGAAACTCTAAGAAAG+GGG 0.633083 1.4:-74648016 MS.gene33984:CDS
ATGAGTTTACAGCCACATTG+TGG 0.634509 1.4:-74646245 MS.gene33984:CDS
ACCGGACGCTTGAAACCCAT+CGG 0.635436 1.4:+74645902 None:intergenic
AAAGGATACGCACAATACAA+AGG 0.637659 1.4:-74647145 MS.gene33984:CDS
CAAGGCTGAAAAGGAATACG+AGG 0.638254 1.4:-74646834 MS.gene33984:CDS
GGATGATCGATCAATATAGG+AGG 0.639611 1.4:+74648231 None:intergenic
TACAAATCAGAGGTTTGACT+AGG 0.642589 1.4:+74646308 None:intergenic
AGATAAGTCCAACTAAATCA+CGG 0.645312 1.4:-74647813 MS.gene33984:CDS
TCATAGCTTAAACGCGATGG+AGG 0.646419 1.4:-74646210 MS.gene33984:CDS
TCAGCTAGCTCTCCGTCAGG+GGG 0.662037 1.4:+74648201 None:intergenic
TTATGAAATTGCAGAATGTG+CGG 0.662542 1.4:-74646339 MS.gene33984:CDS
CGGAGAGCTAGCTGATGGTG+CGG 0.662675 1.4:-74648193 MS.gene33984:CDS
ATGATTGACACCAACAAACG+GGG 0.665562 1.4:+74647393 None:intergenic
TTAGAGGAGAGTATTGTCTG+TGG 0.669312 1.4:-74646632 MS.gene33984:CDS
GACGTCCTCGAACTAATCGT+CGG 0.680634 1.4:-74646127 MS.gene33984:CDS
ATACTTGTTTGTCAAGTACG+TGG 0.696002 1.4:+74647429 None:intergenic
TCAAGCACAATCATTCCATG+CGG 0.697666 1.4:-74646797 MS.gene33984:CDS
TCCCCTCCCACACCACAATG+TGG 0.710147 1.4:+74646233 None:intergenic
ATTGTGTGTATGATTCTCTG+CGG 0.727361 1.4:-74647102 MS.gene33984:CDS
TCCATGGTATGTGATGCCGG+TGG 0.764195 1.4:-74647697 MS.gene33984:CDS
CCGGAATGTCAACAACACAG+AGG 0.797075 1.4:-74646949 MS.gene33984:CDS
AGATTGATATGCATGCACAG+CGG 0.798983 1.4:-74647771 MS.gene33984:CDS
GTATTGTGTTGATTTCAACG+GGG 0.841514 1.4:-74646591 MS.gene33984:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAAAAAATGATTTCT+GGG - chr1.4:74647077-74647096 MS.gene33984:CDS 10.0%
!!! AAATCATTTTTTAAATAACA+TGG + chr1.4:74647075-74647094 None:intergenic 10.0%
!! AATGTAATCACAATAATAAT+AGG + chr1.4:74648038-74648057 None:intergenic 15.0%
!! GTTATTTAAAAAATGATTTC+TGG - chr1.4:74647076-74647095 MS.gene33984:CDS 15.0%
!! AAGAAAAAAACCAATTGTTT+CGG - chr1.4:74647989-74648008 MS.gene33984:CDS 20.0%
!! ACAACATTCATTGAAAAATA+TGG - chr1.4:74646994-74647013 MS.gene33984:CDS 20.0%
!! ATTCATTGAAAAATATGGTT+TGG - chr1.4:74646999-74647018 MS.gene33984:CDS 20.0%
!! TTCATTGAAAAATATGGTTT+GGG - chr1.4:74647000-74647019 MS.gene33984:CDS 20.0%
! AAAATTGCCGAAAAATTGAA+AGG - chr1.4:74646886-74646905 MS.gene33984:CDS 25.0%
! TGTATGAAGAAATGATCAAA+TGG - chr1.4:74647760-74647779 MS.gene33984:CDS 25.0%
! TTACGAAAATCTAACATTTG+TGG - chr1.4:74646375-74646394 MS.gene33984:CDS 25.0%
!! AAAACTAGAAACTCTAAGAA+AGG - chr1.4:74646031-74646050 MS.gene33984:intron 25.0%
!! AAACTAGAAACTCTAAGAAA+GGG - chr1.4:74646032-74646051 MS.gene33984:intron 25.0%
!! TATCGGTATGTCTTTTAAAT+CGG - chr1.4:74645952-74645971 MS.gene33984:intron 25.0%
!!! AAGTATTGTGTTGATTTCAA+CGG - chr1.4:74647456-74647475 MS.gene33984:CDS 25.0%
!!! ACTTTTAACGGATTAAGAAT+TGG + chr1.4:74647891-74647910 None:intergenic 25.0%
!!! GAAATATTTTAACATGCCAT+CGG + chr1.4:74646059-74646078 None:intergenic 25.0%
!!! TTTTTTCACAATCTTTTCAG+TGG + chr1.4:74647955-74647974 None:intergenic 25.0%
AAAGGATGATCGATCAATAT+AGG + chr1.4:74645824-74645843 None:intergenic 30.0%
AAGATGACCAAGTACAAAAA+GGG + chr1.4:74647547-74647566 None:intergenic 30.0%
ACACAATACTTAGTTTCGTT+CGG + chr1.4:74647447-74647466 None:intergenic 30.0%
AGATAAGTCCAACTAAATCA+CGG - chr1.4:74646236-74646255 MS.gene33984:CDS 30.0%
AGATACAAGTAGAATTTCGT+GGG - chr1.4:74647334-74647353 MS.gene33984:CDS 30.0%
CATTTCTTCATACAAATCAG+AGG + chr1.4:74647754-74647773 None:intergenic 30.0%
TAAGATGACCAAGTACAAAA+AGG + chr1.4:74647548-74647567 None:intergenic 30.0%
TATCTCCATATACTTGTCAT+GGG + chr1.4:74647319-74647338 None:intergenic 30.0%
TTAAAAGACATACCGATAGT+AGG + chr1.4:74645950-74645969 None:intergenic 30.0%
TTATGAAATTGCAGAATGTG+CGG - chr1.4:74647710-74647729 MS.gene33984:CDS 30.0%
! AACATCACCAAAGTATTCAT+AGG + chr1.4:74646587-74646606 None:intergenic 30.0%
! AACTAGAAACTCTAAGAAAG+GGG - chr1.4:74646033-74646052 MS.gene33984:intron 30.0%
! CTTTTTCTATGAGATTGACA+TGG - chr1.4:74646507-74646526 MS.gene33984:CDS 30.0%
! TCATGTGCAGAATATTTTCT+GGG - chr1.4:74646540-74646559 MS.gene33984:CDS 30.0%
! TGCGTATCCTTTCAATTTTT+CGG + chr1.4:74646896-74646915 None:intergenic 30.0%
! TTAAAAAATGATTTCTGGGC+CGG - chr1.4:74647081-74647100 MS.gene33984:CDS 30.0%
! TTCATGTGCAGAATATTTTC+TGG - chr1.4:74646539-74646558 MS.gene33984:CDS 30.0%
!! AGTATTGTGTTGATTTCAAC+GGG - chr1.4:74647457-74647476 MS.gene33984:CDS 30.0%
!! ATAACGGTTGCTTTAAAGAA+GGG - chr1.4:74646172-74646191 MS.gene33984:CDS 30.0%
!! GACAACATTTGTTGTTTCAA+AGG + chr1.4:74645909-74645928 None:intergenic 30.0%
!! GACAATTTTGATAAACGATC+AGG - chr1.4:74646300-74646319 MS.gene33984:CDS 30.0%
!!! AATGTGTGGTTGTAGTTTTT+TGG + chr1.4:74647661-74647680 None:intergenic 30.0%
!!! AGTATGAATGCTTTTTTCGA+TGG - chr1.4:74647129-74647148 MS.gene33984:CDS 30.0%
AAAACCACTAGTTGACTGTT+GGG + chr1.4:74648195-74648214 None:intergenic 35.0%
AAAGGATACGCACAATACAA+AGG - chr1.4:74646904-74646923 MS.gene33984:CDS 35.0%
AAATGCAACTGTTATCTCGA+AGG - chr1.4:74647357-74647376 MS.gene33984:CDS 35.0%
AAATGGTCTACCAAGAAGTT+GGG - chr1.4:74647777-74647796 MS.gene33984:CDS 35.0%
AGGTTGTGTTTCCTAATACT+AGG - chr1.4:74646833-74646852 MS.gene33984:CDS 35.0%
ATACTTGTTTGTCAAGTACG+TGG + chr1.4:74646623-74646642 None:intergenic 35.0%
ATCCTACTTTGCTAGAATGA+GGG - chr1.4:74646471-74646490 MS.gene33984:CDS 35.0%
ATTGTGTGTATGATTCTCTG+CGG - chr1.4:74646947-74646966 MS.gene33984:CDS 35.0%
CCACACATTGAAAGATTTGA+AGG - chr1.4:74647672-74647691 MS.gene33984:CDS 35.0%
CCTTCAAATCTTTCAATGTG+TGG + chr1.4:74647675-74647694 None:intergenic 35.0%
CGTGGCAAGATAAATTTAGA+CGG + chr1.4:74647585-74647604 None:intergenic 35.0%
CTAAATTTATCTTGCCACGA+TGG - chr1.4:74647586-74647605 MS.gene33984:CDS 35.0%
CTTGTTTCATGGCATATTCA+CGG + chr1.4:74646000-74646019 None:intergenic 35.0%
GAGATACAAGTAGAATTTCG+TGG - chr1.4:74647333-74647352 MS.gene33984:CDS 35.0%
GATAAATTCACGCCTACTAT+CGG - chr1.4:74645935-74645954 MS.gene33984:intron 35.0%
GGATTAAGAATTGGTTGATC+AGG + chr1.4:74647882-74647901 None:intergenic 35.0%
GTATCTCCATATACTTGTCA+TGG + chr1.4:74647320-74647339 None:intergenic 35.0%
GTGAATATGCCATGAAACAA+GGG - chr1.4:74645999-74646018 MS.gene33984:intron 35.0%
TAAAACCACTAGTTGACTGT+TGG + chr1.4:74648196-74648215 None:intergenic 35.0%
TACAAATCAGAGGTTTGACT+AGG + chr1.4:74647744-74647763 None:intergenic 35.0%
TATCCTACTTTGCTAGAATG+AGG - chr1.4:74646470-74646489 MS.gene33984:CDS 35.0%
TGATGATTGACACCAACAAA+CGG + chr1.4:74646661-74646680 None:intergenic 35.0%
TGATTGCTCAGCTAAGATAA+CGG - chr1.4:74646156-74646175 MS.gene33984:CDS 35.0%
TGTTTCCTAATACTAGGCAT+AGG - chr1.4:74646839-74646858 MS.gene33984:CDS 35.0%
TTGAAATTGTCTCTCGATTG+TGG + chr1.4:74647286-74647305 None:intergenic 35.0%
! ATCTACATACACCGTTTTAG+AGG - chr1.4:74647401-74647420 MS.gene33984:CDS 35.0%
! ATGGTTTGGGTGAAAATGAT+TGG - chr1.4:74647013-74647032 MS.gene33984:CDS 35.0%
! ATTCTAACTTGTGCAAGAGA+AGG - chr1.4:74646079-74646098 MS.gene33984:CDS 35.0%
! CAATCCATTTTACTAGGTTG+CGG - chr1.4:74646685-74646704 MS.gene33984:CDS 35.0%
! GACAATACTCTCCTCTAAAA+CGG + chr1.4:74647415-74647434 None:intergenic 35.0%
! TGAATACCATTTTCGAAGAG+CGG - chr1.4:74647037-74647056 MS.gene33984:CDS 35.0%
! TGTTAGATTTTCGTAACCAC+CGG + chr1.4:74646371-74646390 None:intergenic 35.0%
! TTCACATTTTTACTCCATCG+TGG + chr1.4:74647603-74647622 None:intergenic 35.0%
! TTGGTGGTATGTTTGATTCA+TGG + chr1.4:74646108-74646127 None:intergenic 35.0%
! TTTCACAATCTTTTCAGTGG+TGG + chr1.4:74647952-74647971 None:intergenic 35.0%
!! ATTTGTTGTTTCAAAGGTCC+CGG + chr1.4:74645903-74645922 None:intergenic 35.0%
!! ATTTTGATAAACGATCAGGC+AGG - chr1.4:74646304-74646323 MS.gene33984:CDS 35.0%
!! GATAACGGTTGCTTTAAAGA+AGG - chr1.4:74646171-74646190 MS.gene33984:CDS 35.0%
!! GTATTGTGTTGATTTCAACG+GGG - chr1.4:74647458-74647477 MS.gene33984:CDS 35.0%
!! TGTTTGTTGTTTGAGTTCAG+AGG - chr1.4:74647504-74647523 MS.gene33984:CDS 35.0%
!! TTGCTTTAAAGAAGGGTCTA+TGG - chr1.4:74646179-74646198 MS.gene33984:CDS 35.0%
!! TTGTTGGTGTCAATCATCAT+GGG - chr1.4:74646662-74646681 MS.gene33984:CDS 35.0%
!! TTTGTTGGTGTCAATCATCA+TGG - chr1.4:74646661-74646680 MS.gene33984:CDS 35.0%
!!! GGGTTGATGATTTTGTTGTT+GGG - chr1.4:74646968-74646987 MS.gene33984:CDS 35.0%
!!! TTGATGATTTTGTTGTTGGG+TGG - chr1.4:74646971-74646990 MS.gene33984:CDS 35.0%
AAGACCGCAACCTAGTAAAA+TGG + chr1.4:74646692-74646711 None:intergenic 40.0%
AAGATACCAAGTCCTTTACC+TGG - chr1.4:74646722-74646741 MS.gene33984:CDS 40.0%
AATTGTAACCGATCAGTGCA+AGG - chr1.4:74646792-74646811 MS.gene33984:CDS 40.0%
ACATTACTTCCTATGTCCGA+TGG - chr1.4:74648131-74648150 MS.gene33984:CDS 40.0%
ACTAAATCACGGTTGTTTGC+TGG - chr1.4:74646247-74646266 MS.gene33984:CDS 40.0%
ACTTCGACAACCGAAACAAT+TGG + chr1.4:74648002-74648021 None:intergenic 40.0%
AGATTGATATGCATGCACAG+CGG - chr1.4:74646278-74646297 MS.gene33984:CDS 40.0%
AGGTCTGCCTATGAATACTT+TGG - chr1.4:74646577-74646596 MS.gene33984:CDS 40.0%
ATACTTGTCATGGGTGTACT+CGG + chr1.4:74647310-74647329 None:intergenic 40.0%
ATGAGTTTACAGCCACATTG+TGG - chr1.4:74647804-74647823 MS.gene33984:CDS 40.0%
ATGATTGACACCAACAAACG+GGG + chr1.4:74646659-74646678 None:intergenic 40.0%
CAAAAGAAAGACACCTTCTC+GGG - chr1.4:74646422-74646441 MS.gene33984:CDS 40.0%
CAAATGGTCTACCAAGAAGT+TGG - chr1.4:74647776-74647795 MS.gene33984:CDS 40.0%
CATTACTTCCTATGTCCGAT+GGG - chr1.4:74648132-74648151 MS.gene33984:CDS 40.0%
CGTGAATATGCCATGAAACA+AGG - chr1.4:74645998-74646017 MS.gene33984:intron 40.0%
GAACAAAAGTTTCCGTTCCA+TGG - chr1.4:74646336-74646355 MS.gene33984:CDS 40.0%
GAAGTAGTTAACCGTATCAC+CGG + chr1.4:74648168-74648187 None:intergenic 40.0%
GAGAGATGTCAGAAACTTTG+TGG - chr1.4:74646399-74646418 MS.gene33984:CDS 40.0%
GAGCAATCAAGTTTCTTCGT+TGG + chr1.4:74646145-74646164 None:intergenic 40.0%
GATGATTGACACCAACAAAC+GGG + chr1.4:74646660-74646679 None:intergenic 40.0%
GCAAAAGAAAGACACCTTCT+CGG - chr1.4:74646421-74646440 MS.gene33984:CDS 40.0%
GCAAACAACCGTGATTTAGT+TGG + chr1.4:74646247-74646266 None:intergenic 40.0%
GGATGATCGATCAATATAGG+AGG + chr1.4:74645821-74645840 None:intergenic 40.0%
GTACACCCATGACAAGTATA+TGG - chr1.4:74647311-74647330 MS.gene33984:CDS 40.0%
GTTGGATTCGTCTTCAAAGT+TGG + chr1.4:74646127-74646146 None:intergenic 40.0%
TCAAGCACAATCATTCCATG+CGG - chr1.4:74647252-74647271 MS.gene33984:CDS 40.0%
TCGGACATAGGAAGTAATGT+AGG + chr1.4:74648131-74648150 None:intergenic 40.0%
TGATCGGTTACAATTCCCAT+TGG + chr1.4:74646787-74646806 None:intergenic 40.0%
TGCATCAATGAGCAAAGATG+TGG - chr1.4:74648226-74648245 MS.gene33984:CDS 40.0%
TGGTCATCTTAGAGTGAAAG+AGG - chr1.4:74647557-74647576 MS.gene33984:CDS 40.0%
TGTTTCGAACAACCAGGTAA+AGG + chr1.4:74646737-74646756 None:intergenic 40.0%
TTACCTGGTTGTTCGAAACA+TGG - chr1.4:74646737-74646756 MS.gene33984:CDS 40.0%
TTCCCTCATTCTAGCAAAGT+AGG + chr1.4:74646476-74646495 None:intergenic 40.0%
TTCCTAATACTAGGCATAGG+TGG - chr1.4:74646842-74646861 MS.gene33984:CDS 40.0%
TTGTGTGTATGATTCTCTGC+GGG - chr1.4:74646948-74646967 MS.gene33984:CDS 40.0%
TTTCGGTTGTCGAAGTAAGT+TGG - chr1.4:74648006-74648025 MS.gene33984:CDS 40.0%
! ACTCATTTTGCCCAACTTCT+TGG + chr1.4:74647790-74647809 None:intergenic 40.0%
! AGACATGCCCTTTTTGTACT+TGG - chr1.4:74647537-74647556 MS.gene33984:CDS 40.0%
! AGGAATACGAGGCAGATTTT+CGG - chr1.4:74647226-74647245 MS.gene33984:CDS 40.0%
! CATGGGCAATCCATTTTACT+AGG - chr1.4:74646679-74646698 MS.gene33984:CDS 40.0%
! CCCTTACGTGGTACTTTTAA+CGG + chr1.4:74647903-74647922 None:intergenic 40.0%
! GAATACCATTTTCGAAGAGC+GGG - chr1.4:74647038-74647057 MS.gene33984:CDS 40.0%
! GATTGCAAACCCTTGTTTCA+TGG + chr1.4:74646011-74646030 None:intergenic 40.0%
!! ATTCCTTTTCAGCCTTGTCT+TGG + chr1.4:74647212-74647231 None:intergenic 40.0%
!! CCGTTAAAAGTACCACGTAA+GGG - chr1.4:74647900-74647919 MS.gene33984:CDS 40.0%
!! TCCGTTAAAAGTACCACGTA+AGG - chr1.4:74647899-74647918 MS.gene33984:CDS 40.0%
!! TTAGAGGAGAGTATTGTCTG+TGG - chr1.4:74647417-74647436 MS.gene33984:CDS 40.0%
!!! CGGGTTGATGATTTTGTTGT+TGG - chr1.4:74646967-74646986 MS.gene33984:CDS 40.0%
ACTCCAAGACAAGGCTGAAA+AGG - chr1.4:74647206-74647225 MS.gene33984:CDS 45.0%
CAAGCACAATCATTCCATGC+GGG - chr1.4:74647253-74647272 MS.gene33984:CDS 45.0%
CAAGGCTGAAAAGGAATACG+AGG - chr1.4:74647215-74647234 MS.gene33984:CDS 45.0%
CACCACCTATGCCTAGTATT+AGG + chr1.4:74646847-74646866 None:intergenic 45.0%
CATCCTTTATTACCCCCTGA+CGG - chr1.4:74645836-74645855 MS.gene33984:CDS 45.0%
CTATGAGATTGACATGGACG+AGG - chr1.4:74646513-74646532 MS.gene33984:CDS 45.0%
GAACAACCAGGTAAAGGACT+TGG + chr1.4:74646731-74646750 None:intergenic 45.0%
GGATCATAGCTTAAACGCGA+TGG - chr1.4:74647836-74647855 MS.gene33984:CDS 45.0%
GGATTCGTCTTCAAAGTTGG+TGG + chr1.4:74646124-74646143 None:intergenic 45.0%
GGCTATGCAGAATGCAATTC+AGG - chr1.4:74646813-74646832 MS.gene33984:CDS 45.0%
GGTTGTTCGAAACATGGCTA+AGG - chr1.4:74646743-74646762 MS.gene33984:CDS 45.0%
TAAACGATCAGGCAGGTGTA+CGG - chr1.4:74646311-74646330 MS.gene33984:CDS 45.0%
TAGCCATGTTTCGAACAACC+AGG + chr1.4:74646743-74646762 None:intergenic 45.0%
TCATAGCTTAAACGCGATGG+AGG - chr1.4:74647839-74647858 MS.gene33984:CDS 45.0%
TCATCCCAACAGTCAACTAG+TGG - chr1.4:74648188-74648207 MS.gene33984:CDS 45.0%
TCGGTTACAATTCCCATTGG+AGG + chr1.4:74646784-74646803 None:intergenic 45.0%
TCTTTCCCGCTCTTCGAAAA+TGG + chr1.4:74647046-74647065 None:intergenic 45.0%
TTACAGCCACATTGTGGTGT+GGG - chr1.4:74647810-74647829 MS.gene33984:CDS 45.0%
TTTACAGCCACATTGTGGTG+TGG - chr1.4:74647809-74647828 MS.gene33984:CDS 45.0%
TTTCGAAGAGCGGGAAAGAT+GGG - chr1.4:74647047-74647066 MS.gene33984:CDS 45.0%
! CCTCTGTGTTGTTGACATTC+CGG + chr1.4:74647103-74647122 None:intergenic 45.0%
! TCTCGAAGGTGTGACTACAT+TGG - chr1.4:74647371-74647390 MS.gene33984:CDS 45.0%
! TTTTCGAAGAGCGGGAAAGA+TGG - chr1.4:74647046-74647065 MS.gene33984:CDS 45.0%
AAACGATCAGGCAGGTGTAC+GGG - chr1.4:74646312-74646331 MS.gene33984:CDS 50.0%
ACACCAACAAACGGGGCAAA+TGG + chr1.4:74646652-74646671 None:intergenic 50.0%
AGAAAGACACCTTCTCGGGA+AGG - chr1.4:74646426-74646445 MS.gene33984:CDS 50.0%
ATGTCTTCGAGGCCTCCAAT+GGG - chr1.4:74646769-74646788 MS.gene33984:CDS 50.0%
ATTGTGGAGTTTGACCCGCA+TGG + chr1.4:74647270-74647289 None:intergenic 50.0%
CCGGAATGTCAACAACACAG+AGG - chr1.4:74647100-74647119 MS.gene33984:CDS 50.0%
CGGCATCACATACCATGGAA+CGG + chr1.4:74646351-74646370 None:intergenic 50.0%
CGTTCCATGGTATGTGATGC+CGG - chr1.4:74646349-74646368 MS.gene33984:CDS 50.0%
CTAGGCATAGGTGGTGTCTA+TGG - chr1.4:74646851-74646870 MS.gene33984:CDS 50.0%
GACGTCCTCGAACTAATCGT+CGG - chr1.4:74647922-74647941 MS.gene33984:CDS 50.0%
GCTTGAAACCCATCGGACAT+AGG + chr1.4:74648143-74648162 None:intergenic 50.0%
GGCTAAGGTGTATGTCTTCG+AGG - chr1.4:74646758-74646777 MS.gene33984:CDS 50.0%
GTTTCAAGCGTCCGGTGATA+CGG - chr1.4:74648154-74648173 MS.gene33984:CDS 50.0%
TATGTCTTCGAGGCCTCCAA+TGG - chr1.4:74646768-74646787 MS.gene33984:CDS 50.0%
TCTCCGTCAGGGGGTAATAA+AGG + chr1.4:74645842-74645861 None:intergenic 50.0%
! AACTCTAAGAAAGGGGCCGA+TGG - chr1.4:74646040-74646059 MS.gene33984:intron 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG - chr1.4:74646646-74646665 MS.gene33984:CDS 50.0%
! CGTGTTGACGTTTCGGATTC+CGG - chr1.4:74645881-74645900 MS.gene33984:CDS 50.0%
! CTGCATAGCCTTGCACTGAT+CGG + chr1.4:74646803-74646822 None:intergenic 50.0%
! GACAACCGACGATTAGTTCG+AGG + chr1.4:74647930-74647949 None:intergenic 50.0%
! GTGTTGACGTTTCGGATTCC+GGG - chr1.4:74645882-74645901 MS.gene33984:CDS 50.0%
ACCACCGGCATCACATACCA+TGG + chr1.4:74646356-74646375 None:intergenic 55.0%
ACCGGACGCTTGAAACCCAT+CGG + chr1.4:74648150-74648169 None:intergenic 55.0%
AGACACCTTCTCGGGAAGGA+AGG - chr1.4:74646430-74646449 MS.gene33984:CDS 55.0%
AGCCACATTGTGGTGTGGGA+GGG - chr1.4:74647814-74647833 MS.gene33984:CDS 55.0%
CATCTCCTTCCTTCCCGAGA+AGG + chr1.4:74646438-74646457 None:intergenic 55.0%
CGACAACGCACTCCAAGACA+AGG - chr1.4:74647197-74647216 MS.gene33984:CDS 55.0%
CTTCTCGGGAAGGAAGGAGA+TGG - chr1.4:74646436-74646455 MS.gene33984:CDS 55.0%
TCCATGGTATGTGATGCCGG+TGG - chr1.4:74646352-74646371 MS.gene33984:CDS 55.0%
TCTGGGCTGATGCACGTAGT+AGG - chr1.4:74646557-74646576 MS.gene33984:CDS 55.0%
! ATCAGCTAGCTCTCCGTCAG+GGG + chr1.4:74645852-74645871 None:intergenic 55.0%
! CATCAGCTAGCTCTCCGTCA+GGG + chr1.4:74645853-74645872 None:intergenic 55.0%
! TCCGATGGGTTTCAAGCGTC+CGG - chr1.4:74648146-74648165 MS.gene33984:CDS 55.0%
! TGCGGCTCGTGTTGACGTTT+CGG - chr1.4:74645874-74645893 MS.gene33984:CDS 55.0%
CAGCCACATTGTGGTGTGGG+AGG - chr1.4:74647813-74647832 MS.gene33984:CDS 60.0%
CCTGACGGAGAGCTAGCTGA+TGG - chr1.4:74645851-74645870 MS.gene33984:CDS 60.0%
CGGGAAGGAAGGAGATGGCA+GGG - chr1.4:74646441-74646460 MS.gene33984:CDS 60.0%
GCCACATTGTGGTGTGGGAG+GGG - chr1.4:74647815-74647834 MS.gene33984:CDS 60.0%
GTTCGAGGACGTCCCTTACG+TGG + chr1.4:74647915-74647934 None:intergenic 60.0%
TCCCCTCCCACACCACAATG+TGG + chr1.4:74647819-74647838 None:intergenic 60.0%
TCGGGAAGGAAGGAGATGGC+AGG - chr1.4:74646440-74646459 MS.gene33984:CDS 60.0%
! CCATCAGCTAGCTCTCCGTC+AGG + chr1.4:74645854-74645873 None:intergenic 60.0%
! CGGAGAGCTAGCTGATGGTG+CGG - chr1.4:74645856-74645875 MS.gene33984:CDS 60.0%
! TCAGCTAGCTCTCCGTCAGG+GGG + chr1.4:74645851-74645870 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74645806 74648265 74645806 ID=MS.gene33984
chr1.4 mRNA 74645806 74648265 74645806 ID=MS.gene33984.t1;Parent=MS.gene33984
chr1.4 exon 74646052 74648265 74646052 ID=MS.gene33984.t1.exon1;Parent=MS.gene33984.t1
chr1.4 CDS 74646052 74648265 74646052 ID=cds.MS.gene33984.t1;Parent=MS.gene33984.t1
chr1.4 exon 74645806 74645943 74645806 ID=MS.gene33984.t1.exon2;Parent=MS.gene33984.t1
chr1.4 CDS 74645806 74645943 74645806 ID=cds.MS.gene33984.t1;Parent=MS.gene33984.t1
Gene Sequence

>MS.gene33984

ATGGCTGATGGTCCTCCTATATTGATCGATCATCCTTTATTACCCCCTGACGGAGAGCTAGCTGATGGTGCGGCTCGTGTTGACGTTTCGGATTCCGGGACCTTTGAAACAACAAATGTTGTCGCAACAGATAAATTCACGCCTACTATCGGTATGTCTTTTAAATCGGTTGCAGATTGTAAGTCTTTTTACCGTGAATATGCCATGAAACAAGGGTTTGCAATCAAAACTAGAAACTCTAAGAAAGGGGCCGATGGCATGTTAAAATATTTCATTCTAACTTGTGCAAGAGAAGGTCACCATGAATCAAACATACCACCAACTTTGAAGACGAATCCAACGAAGAAACTTGATTGCTCAGCTAAGATAACGGTTGCTTTAAAGAAGGGTCTATGGACTATTTTGTCTTTTAATTCATGTCATAATCATGAGATAAGTCCAACTAAATCACGGTTGTTTGCTGGCAATAAGAAGATTGATATGCATGCACAGCGGACAATTTTGATAAACGATCAGGCAGGTGTACGGGTGAACAAAAGTTTCCGTTCCATGGTATGTGATGCCGGTGGTTACGAAAATCTAACATTTGTGGAGAGAGATGTCAGAAACTTTGTGGCAAAAGAAAGACACCTTCTCGGGAAGGAAGGAGATGGCAGGGCACTTTTATCCTACTTTGCTAGAATGAGGGAATGCAACAAAGACTTTTTCTATGAGATTGACATGGACGAGGACTTTCATGTGCAGAATATTTTCTGGGCTGATGCACGTAGTAGGTCTGCCTATGAATACTTTGGTGATGTTGTCACATTCGATACCACGTACTTGACAAACAAGTATGACATGCCATTTGCCCCGTTTGTTGGTGTCAATCATCATGGGCAATCCATTTTACTAGGTTGCGGTCTTTTATCATCTGAAGATACCAAGTCCTTTACCTGGTTGTTCGAAACATGGCTAAGGTGTATGTCTTCGAGGCCTCCAATGGGAATTGTAACCGATCAGTGCAAGGCTATGCAGAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGAAAAATTGCCGAAAAATTGAAAGGATACGCACAATACAAAGGAATAAAAAATGATATGAAATATTGTGTGTATGATTCTCTGCGGGTTGATGATTTTGTTGTTGGGTGGACAACATTCATTGAAAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAAAATGATTTCTGGGCCGGAATGTCAACAACACAGAGGAGTGAAAGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTAAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCAGATTTTCGGTCTTCAAGCACAATCATTCCATGCGGGTCAAACTCCACAATCGAGAGACAATTTCAAGCCGAGTACACCCATGACAAGTATATGGAGATACAAGTAGAATTTCGTGGGAAATGCAACTGTTATCTCGAAGGTGTGACTACATTGGAATTTACATCTACATACACCGTTTTAGAGGAGAGTATTGTCTGTGGTAAACCGAACGAAACTAAGTATTGTGTTGATTTCAACGGGGAAGACAATAATGTTACGTGCACTTGTTTGTTGTTTGAGTTCAGAGGCATTTTGTGTAGACATGCCCTTTTTGTACTTGGTCATCTTAGAGTGAAAGAGGTTCCGTCTAAATTTATCTTGCCACGATGGAGTAAAAATGTGAAGAGAAAATATGCACACATGAAGACAAGTTACAATGCCAAAAAACTACAACCACACATTGAAAGATTTGAAGGTCTTTGCAAGACATTTTATGAAATTGCAGAATGTGCGGCTGAGACACCTAGTCAAACCTCTGATTTGTATGAAGAAATGATCAAATGGTCTACCAAGAAGTTGGGCAAAATGAGTTTACAGCCACATTGTGGTGTGGGAGGGGATCATAGCTTAAACGCGATGGAGGACTCATTATCACAGCAACCTGATCAACCAATTCTTAATCCGTTAAAAGTACCACGTAAGGGACGTCCTCGAACTAATCGTCGGTTGTCCACCACTGAAAAGATTGTGAAAAAAAGAAGCAAAATGCAAAGAAAAAAACCAATTGTTTCGGTTGTCGAAGTAAGTTGGTGTTAACCTATTATTATTGTGATTACATTTGTTTTTTTAATAATAATACAATGTTAATTATGTTAAATGTAATTAATATTTCACTCTGTAATACTACAGCCTACATTACTTCCTATGTCCGATGGGTTTCAAGCGTCCGGTGATACGGTTAACTACTTCTCATCCCAACAGTCAACTAGTGGTTTTATGTCATTACTTGCATCAATGAGCAAAGATGTGGACGCAGCTGAATTTTAA
Protein sequence

>MS.gene33984.t1

MADGPPILIDHPLLPPDGELADGAARVDVSDSGTFETTNVVATDKFTPTIGMSFKSVADCKSFYREYAMKQGFAIKTRNSKKGADGMLKYFILTCAREGHHESNIPPTLKTNPTKKLDCSAKITVALKKGLWTILSFNSCHNHEISPTKSRLFAGNKKIDMHAQRTILINDQAGVRVNKSFRSMVCDAGGYENLTFVERDVRNFVAKERHLLGKEGDGRALLSYFARMRECNKDFFYEIDMDEDFHVQNIFWADARSRSAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSILLGCGLLSSEDTKSFTWLFETWLRCMSSRPPMGIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKIAEKLKGYAQYKGIKNDMKYCVYDSLRVDDFVVGWTTFIEKYGLGENDWLNTIFEERERWVPCYLKNDFWAGMSTTQRSESMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPCGSNSTIERQFQAEYTHDKYMEIQVEFRGKCNCYLEGVTTLEFTSTYTVLEESIVCGKPNETKYCVDFNGEDNNVTCTCLLFEFRGILCRHALFVLGHLRVKEVPSKFILPRWSKNVKRKYAHMKTSYNAKKLQPHIERFEGLCKTFYEIAECAAETPSQTSDLYEEMIKWSTKKLGKMSLQPHCGVGGDHSLNAMEDSLSQQPDQPILNPLKVPRKGRPRTNRRLSTTEKIVKKRSKMQRKKPIVSVVEPTLLPMSDGFQASGDTVNYFSSQQSTSGFMSLLASMSKDVDAAEF