AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34000.t1 MTR_1g095850 98.696 230 2 1 1 230 1 229 4.62e-166 457
MS.gene34000.t1 MTR_2g093310 62.911 213 56 6 26 230 1 198 1.08e-82 245
MS.gene34000.t1 MTR_4g060950 67.416 178 45 7 64 230 15 190 5.89e-77 230
MS.gene34000.t1 MTR_2g068760 70.909 110 32 0 71 180 11 120 8.15e-54 170
MS.gene34000.t1 MTR_4g083680 67.890 109 35 0 72 180 14 122 3.23e-51 164
MS.gene34000.t1 MTR_3g071590 71.845 103 29 0 72 174 7 109 1.50e-50 162
MS.gene34000.t1 MTR_5g083230 65.487 113 39 0 72 184 5 117 2.29e-50 162
MS.gene34000.t1 MTR_3g031660 66.055 109 37 0 72 180 6 114 1.31e-49 160
MS.gene34000.t1 MTR_5g083960 54.386 114 52 0 71 184 37 150 2.86e-41 139
MS.gene34000.t1 MTR_3g077240 59.048 105 43 0 72 176 9 113 5.92e-41 139
MS.gene34000.t1 MTR_3g073690 58.824 102 42 0 72 173 8 109 9.25e-41 138
MS.gene34000.t1 MTR_4g105170 59.223 103 42 0 72 174 10 112 2.52e-40 137
MS.gene34000.t1 MTR_6g005070 52.174 115 55 0 72 186 8 122 3.45e-40 135
MS.gene34000.t1 MTR_7g033800 58.252 103 43 0 72 174 10 112 1.28e-39 134
MS.gene34000.t1 MTR_3g094690 52.101 119 56 1 64 181 37 155 1.28e-39 136
MS.gene34000.t1 MTR_5g017950 57.843 102 43 0 72 173 7 108 1.33e-38 136
MS.gene34000.t1 MTR_5g075020 59.459 111 45 0 71 181 41 151 2.15e-38 133
MS.gene34000.t1 MTR_6g005080 54.286 105 48 0 72 176 9 113 3.61e-38 130
MS.gene34000.t1 MTR_5g075020 59.459 111 45 0 71 181 45 155 4.32e-38 132
MS.gene34000.t1 MTR_3g094690 51.261 119 56 2 64 181 37 154 5.08e-37 130
MS.gene34000.t1 MTR_3g452660 53.774 106 49 0 72 177 6 111 1.17e-36 130
MS.gene34000.t1 MTR_5g083010 55.882 102 45 0 72 173 10 111 4.57e-36 125
MS.gene34000.t1 MTR_3g071420 56.566 99 43 0 72 170 13 111 2.24e-35 124
MS.gene34000.t1 MTR_7g028905 45.690 116 61 1 69 182 1 116 4.35e-33 116
MS.gene34000.t1 MTR_8g040900 57.843 102 43 0 72 173 6 107 4.53e-32 117
MS.gene34000.t1 MTR_7g074990 50.000 102 50 1 73 173 20 121 1.96e-31 115
MS.gene34000.t1 MTR_7g096530 51.456 103 49 1 72 173 13 115 2.89e-31 114
MS.gene34000.t1 MTR_8g036105 50.943 106 51 1 72 176 17 122 3.55e-31 114
MS.gene34000.t1 MTR_6g027710 43.697 119 64 2 70 186 37 154 4.28e-31 114
MS.gene34000.t1 MTR_8g079660 48.077 104 54 0 70 173 5 108 2.53e-30 113
MS.gene34000.t1 MTR_1g070220 44.531 128 58 2 48 174 2 117 3.67e-30 111
MS.gene34000.t1 MTR_8g036085 48.544 103 52 1 72 173 22 124 7.58e-30 111
MS.gene34000.t1 MTR_1g070205 51.961 102 46 2 73 171 12 113 1.88e-29 110
MS.gene34000.t1 MTR_8g079620 48.077 104 54 0 70 173 5 108 4.46e-29 109
MS.gene34000.t1 MTR_5g093010 41.905 105 61 0 69 173 1 105 5.80e-29 106
MS.gene34000.t1 MTR_7g075230 50.476 105 49 2 72 173 11 115 1.54e-28 107
MS.gene34000.t1 MTR_7g096610 51.546 97 46 1 72 167 7 103 2.25e-28 107
MS.gene34000.t1 MTR_6g027700 37.671 146 79 4 70 212 4 140 6.45e-27 103
MS.gene34000.t1 MTR_8g079580 46.226 106 56 1 70 174 5 110 7.22e-27 103
MS.gene34000.t1 MTR_5g080470 46.875 96 50 1 72 166 6 101 4.52e-26 100
MS.gene34000.t1 MTR_6g011250 42.609 115 61 2 74 183 10 124 2.45e-25 98.2
MS.gene34000.t1 MTR_6g011200 42.623 122 59 3 74 187 10 128 1.79e-24 97.8
MS.gene34000.t1 MTR_4g107450 43.000 100 57 0 74 173 11 110 1.45e-23 96.3
MS.gene34000.t1 MTR_6g011230 35.762 151 90 4 74 224 12 155 1.10e-21 89.4
MS.gene34000.t1 MTR_4g099230 40.000 115 58 3 74 183 3 111 1.40e-20 85.1
MS.gene34000.t1 MTR_8g017090 39.000 100 61 0 74 173 13 112 1.55e-19 85.5
MS.gene34000.t1 MTR_2g100020 40.860 93 55 0 74 166 20 112 6.44e-19 83.2
MS.gene34000.t1 MTR_5g085390 38.614 101 61 1 74 173 10 110 5.05e-16 74.7
MS.gene34000.t1 MTR_4g088035 32.258 93 63 0 69 161 2 94 1.46e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34000.t1 AT1G07900 68.072 166 44 5 69 230 29 189 2.80e-74 223
MS.gene34000.t1 AT2G28500 63.187 182 47 4 69 230 51 232 2.85e-71 217
MS.gene34000.t1 AT2G30130 68.519 108 34 0 72 179 7 114 3.48e-50 162
MS.gene34000.t1 AT1G16530 65.421 107 36 1 71 176 12 118 2.77e-47 154
MS.gene34000.t1 AT1G31320 67.273 110 36 0 72 181 12 121 2.18e-43 144
MS.gene34000.t1 AT5G63090 60.194 103 41 0 72 174 10 112 4.34e-41 139
MS.gene34000.t1 AT5G63090 60.194 103 41 0 72 174 10 112 4.34e-41 139
MS.gene34000.t1 AT5G63090 60.194 103 41 0 72 174 10 112 4.34e-41 139
MS.gene34000.t1 AT5G63090 60.194 103 41 0 72 174 10 112 4.34e-41 139
MS.gene34000.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.28e-41 142
MS.gene34000.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.28e-41 142
MS.gene34000.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.28e-41 142
MS.gene34000.t1 AT2G30340 52.632 114 54 0 71 184 50 163 9.26e-41 140
MS.gene34000.t1 AT2G30340 52.632 114 54 0 71 184 51 164 9.70e-41 140
MS.gene34000.t1 AT3G27650 58.654 104 43 0 72 175 38 141 2.84e-40 136
MS.gene34000.t1 AT5G66870 57.843 102 43 0 72 173 6 107 1.28e-38 136
MS.gene34000.t1 AT2G40470 49.296 142 68 2 61 200 6 145 4.93e-38 131
MS.gene34000.t1 AT2G40470 49.296 142 68 2 61 200 31 170 9.95e-38 131
MS.gene34000.t1 AT3G26660 56.000 100 44 0 74 173 6 105 1.70e-37 127
MS.gene34000.t1 AT3G26620 56.000 100 44 0 74 173 6 105 1.74e-37 127
MS.gene34000.t1 AT3G11090 54.369 103 47 0 72 174 10 112 7.43e-37 127
MS.gene34000.t1 AT1G65620 59.804 102 41 0 72 173 8 109 2.29e-33 119
MS.gene34000.t1 AT1G65620 59.804 102 41 0 72 173 8 109 2.29e-33 119
MS.gene34000.t1 AT1G65620 59.804 102 41 0 72 173 8 109 2.29e-33 119
MS.gene34000.t1 AT1G65620 59.804 102 41 0 72 173 8 109 2.29e-33 119
MS.gene34000.t1 AT1G65620 59.804 102 41 0 72 173 8 109 2.29e-33 119
MS.gene34000.t1 AT4G00210 52.336 107 50 1 72 177 10 116 2.93e-33 120
MS.gene34000.t1 AT3G03760 48.214 112 57 1 72 182 50 161 4.36e-33 120
MS.gene34000.t1 AT4G00220 50.980 102 49 1 76 176 20 121 1.29e-30 113
MS.gene34000.t1 AT2G31310 48.077 104 53 1 70 172 4 107 2.48e-29 108
MS.gene34000.t1 AT2G45410 54.545 99 44 1 76 173 19 117 3.35e-29 108
MS.gene34000.t1 AT2G42430 46.667 105 55 1 72 175 14 118 4.03e-29 109
MS.gene34000.t1 AT2G42440 54.167 96 43 1 72 166 6 101 5.30e-29 109
MS.gene34000.t1 AT2G45420 49.020 102 51 1 76 176 40 141 3.47e-28 107
MS.gene34000.t1 AT3G58190 49.474 95 47 1 73 166 11 105 1.97e-26 102
MS.gene34000.t1 AT3G50510 45.192 104 57 0 70 173 9 112 4.15e-26 100
MS.gene34000.t1 AT3G50510 45.192 104 57 0 70 173 9 112 4.15e-26 100
MS.gene34000.t1 AT5G06080 44.340 106 58 1 72 176 6 111 3.41e-25 97.8
MS.gene34000.t1 AT1G72980 45.263 95 52 0 72 166 12 106 5.10e-24 95.9
MS.gene34000.t1 AT5G35900 41.000 100 59 0 74 173 6 105 4.46e-23 93.2
MS.gene34000.t1 AT3G47870 41.176 102 60 0 72 173 35 136 3.57e-22 93.2
MS.gene34000.t1 AT3G13850 43.011 93 53 0 74 166 37 129 5.28e-22 91.7
MS.gene34000.t1 AT4G22700 37.624 101 61 2 74 173 6 105 1.14e-15 73.2
MS.gene34000.t1 AT1G06280 38.824 85 49 1 73 154 24 108 1.23e-15 73.2
MS.gene34000.t1 AT2G19820 38.202 89 54 1 72 159 11 99 1.07e-14 68.6
MS.gene34000.t1 AT5G15060 42.857 77 40 2 71 144 15 90 8.93e-12 61.2
MS.gene34000.t1 AT1G36000 33.333 87 58 0 73 159 9 95 4.93e-11 58.9

Find 62 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTGCTTGGGAGCCTCTT+TGG 0.215297 1.4:-74442169 MS.gene34000:CDS
ACCATTGCTCATAGAGTCTT+TGG 0.230237 1.4:-74443397 MS.gene34000:CDS
AGGATAAGGGACCCAGTTTA+TGG 0.252541 1.4:-74442416 MS.gene34000:CDS
GCACCTGCACACCCATAAAC+TGG 0.308339 1.4:+74442404 None:intergenic
GCACAAGGGCTAACAATAAT+TGG 0.330791 1.4:+74443502 None:intergenic
CACTAGCTCAGCTTGTGCTT+TGG 0.331624 1.4:+74442334 None:intergenic
GCATCTGCTCTCTGAGGTTC+AGG 0.338371 1.4:+74442470 None:intergenic
CAACATCATTAAGTTCTTGC+AGG 0.371270 1.4:-74443368 MS.gene34000:intron
GTGCCACTAAATTTGCTTGT+TGG 0.373985 1.4:+74442300 None:intergenic
GGTGGAGGAGATGGTGGTTG+AGG 0.390187 1.4:+74443526 None:intergenic
GGAGGAGAATATTGAGAAGC+TGG 0.397244 1.4:+74443559 None:intergenic
ATGAAGCAAATGCAAGGATA+AGG 0.416793 1.4:-74442430 MS.gene34000:CDS
CCTGCGACGGAGAATCTTGC+AGG 0.417127 1.4:+74443477 None:intergenic
ACTCATATCAACATGAGGTT+GGG 0.426563 1.4:+74442217 None:intergenic
CCTGCAAGATTCTCCGTCGC+AGG 0.451176 1.4:-74443477 MS.gene34000:CDS
GGATAAGGGACCCAGTTTAT+GGG 0.452807 1.4:-74442415 MS.gene34000:CDS
GTGTGAATGGGAACATGAAT+TGG 0.456087 1.4:+74443655 None:intergenic
TTGCCAACAAGCAAATTTAG+TGG 0.460480 1.4:-74442303 MS.gene34000:CDS
TGAAGCAAATGCAAGGATAA+GGG 0.482905 1.4:-74442429 MS.gene34000:CDS
GACCCAGTTTATGGGTGTGC+AGG 0.487417 1.4:-74442407 MS.gene34000:CDS
GTTTGATAGCATGGTGTGAA+TGG 0.487876 1.4:+74443642 None:intergenic
TTTAGTGGCACTAATTTGCA+TGG 0.489022 1.4:-74442288 MS.gene34000:CDS
AGAATCTTGCAGGCAGCACA+AGG 0.497089 1.4:+74443487 None:intergenic
GGATCAGTTGGAGGAAAATA+CGG 0.507188 1.4:+74443430 None:intergenic
ATAATTGGTGGTGGAGGAGA+TGG 0.508722 1.4:+74443517 None:intergenic
GAATATTGAGAAGCTGGTGA+AGG 0.509511 1.4:+74443565 None:intergenic
AACTCATATCAACATGAGGT+TGG 0.513338 1.4:+74442216 None:intergenic
TAGATGAGGAGGAGAAGTAG+TGG 0.517097 1.4:+74443684 None:intergenic
GCTGGTGAAGGGAAAGGTGT+GGG 0.521269 1.4:+74443577 None:intergenic
CTCATATCAACATGAGGTTG+GGG 0.524829 1.4:+74442218 None:intergenic
TGGTGGTTGAGGAGCAGCAG+AGG 0.528834 1.4:+74443537 None:intergenic
AAAGGTGTGGGAGATTGTAA+AGG 0.533363 1.4:+74443589 None:intergenic
TGAGAAGCTGGTGAAGGGAA+AGG 0.534082 1.4:+74443571 None:intergenic
TGTGAATGGGAACATGAATT+GGG 0.535599 1.4:+74443656 None:intergenic
ATTGGGATTGGTAGATGAGG+AGG 0.537299 1.4:+74443673 None:intergenic
AGCTGGTGAAGGGAAAGGTG+TGG 0.541852 1.4:+74443576 None:intergenic
AATTAATTTCATGTCCAAAG+AGG 0.542951 1.4:+74442155 None:intergenic
ATGGGAACATGAATTGGGAT+TGG 0.544335 1.4:+74443661 None:intergenic
TTAATCATCATGTCATGGTC+TGG 0.552049 1.4:+74442245 None:intergenic
GGAGGTGAAGAAGATGAAGA+TGG 0.556111 1.4:+74443610 None:intergenic
GGTGTGGGAGATTGTAAAGG+AGG 0.557869 1.4:+74443592 None:intergenic
CACCTGCACACCCATAAACT+GGG 0.560148 1.4:+74442405 None:intergenic
ATTGGTGGTGGAGGAGATGG+TGG 0.560667 1.4:+74443520 None:intergenic
GGGCTAACAATAATTGGTGG+TGG 0.570890 1.4:+74443508 None:intergenic
TGTAGTAGAGTTTGATAGCA+TGG 0.578297 1.4:+74443633 None:intergenic
TGAATTGGGATTGGTAGATG+AGG 0.583122 1.4:+74443670 None:intergenic
TTTGATAGCATGGTGTGAAT+GGG 0.585620 1.4:+74443643 None:intergenic
TCCAAAGACTCTATGAGCAA+TGG 0.589978 1.4:+74443396 None:intergenic
CTAACAATAATTGGTGGTGG+AGG 0.590976 1.4:+74443511 None:intergenic
AACTTGAGTGGATCAGTTGG+AGG 0.595286 1.4:+74443421 None:intergenic
CTTACTGCATCTGCTCTCTG+AGG 0.598505 1.4:+74442464 None:intergenic
GTGAACTTGAGTGGATCAGT+TGG 0.601167 1.4:+74443418 None:intergenic
AATATTGAGAAGCTGGTGAA+GGG 0.602247 1.4:+74443566 None:intergenic
GCTGATTAATCATCATGTCA+TGG 0.608852 1.4:+74442240 None:intergenic
GGTTGAGGAGCAGCAGAGGG+AGG 0.611544 1.4:+74443541 None:intergenic
GGTGGTTGAGGAGCAGCAGA+GGG 0.614036 1.4:+74443538 None:intergenic
AGCAGATGCAGTAAGCAGCA+TGG 0.619103 1.4:-74442456 MS.gene34000:CDS
TGAGCAATGGTGAACTTGAG+TGG 0.624171 1.4:+74443409 None:intergenic
CAAGGGCTAACAATAATTGG+TGG 0.638801 1.4:+74443505 None:intergenic
TGGTTTATGAAGCAAATGCA+AGG 0.645836 1.4:-74442436 MS.gene34000:CDS
AAGCAACTCATATCAACATG+AGG 0.663119 1.4:+74442212 None:intergenic
GAATCTTGCAGGCAGCACAA+GGG 0.696277 1.4:+74443488 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTTTTTTCAACATAT+TGG - chr1.4:74442588-74442607 MS.gene34000:intron 10.0%
!! AATAAAATGTACTATAAGTT+AGG + chr1.4:74443193-74443212 None:intergenic 15.0%
!! AATAAATTTAGAGAAAATCT+TGG - chr1.4:74443222-74443241 MS.gene34000:intron 15.0%
!! TAGTTATAATAAGTGAAAAA+TGG - chr1.4:74443245-74443264 MS.gene34000:intron 15.0%
!!! TAAAATTCCATTAATTTTTC+AGG - chr1.4:74443368-74443387 MS.gene34000:intron 15.0%
!! ATCACAAGTCAAAAATAATT+AGG - chr1.4:74443053-74443072 MS.gene34000:intron 20.0%
!! CATGAAAAAAATAAAAGCTA+TGG - chr1.4:74442939-74442958 MS.gene34000:intron 20.0%
!! TGTAAAAGATAAAAAGTTAG+TGG + chr1.4:74442921-74442940 None:intergenic 20.0%
!! TGTATGATCTTAAAACAAAT+TGG - chr1.4:74442620-74442639 MS.gene34000:intron 20.0%
!!! TATTTTAGTAATGTGCAAAT+TGG + chr1.4:74442573-74442592 None:intergenic 20.0%
! AAACTTATGTGCATTTGTTT+AGG + chr1.4:74443282-74443301 None:intergenic 25.0%
! AAAGATAAAAAGTTAGTGGT+TGG + chr1.4:74442917-74442936 None:intergenic 25.0%
! AATGAAAAGATTCCAAAATG+TGG + chr1.4:74443082-74443101 None:intergenic 25.0%
! ATGAAAAGATTCCAAAATGT+GGG + chr1.4:74443081-74443100 None:intergenic 25.0%
! ATTAAGCCATGATTTGAAAA+TGG + chr1.4:74443308-74443327 None:intergenic 25.0%
! TTCATTATGTCTAACGATTA+TGG - chr1.4:74443096-74443115 MS.gene34000:intron 25.0%
!! AATTGTCTCATAACAGTTTT+AGG - chr1.4:74442740-74442759 MS.gene34000:intron 25.0%
!! ATTGTCTCATAACAGTTTTA+GGG - chr1.4:74442741-74442760 MS.gene34000:intron 25.0%
!! TTTTGTCCATTTTCAAATCA+TGG - chr1.4:74443299-74443318 MS.gene34000:intron 25.0%
!!! ACATGATGATTTTTTCTTGT+TGG + chr1.4:74442544-74442563 None:intergenic 25.0%
!!! TTTTAGAGGACAACAATTTT+GGG - chr1.4:74443676-74443695 MS.gene34000:CDS 25.0%
!!! TTTTTAGAGGACAACAATTT+TGG - chr1.4:74443675-74443694 MS.gene34000:CDS 25.0%
AAGATATAGTGGTTACACTA+TGG + chr1.4:74443033-74443052 None:intergenic 30.0%
GGTAGTTCCTGAAAAATTAA+TGG + chr1.4:74443378-74443397 None:intergenic 30.0%
TGGATGGAAAAGATAACAAA+AGG - chr1.4:74442793-74442812 MS.gene34000:intron 30.0%
! TGACTTGTGATAAGATATAG+TGG + chr1.4:74443044-74443063 None:intergenic 30.0%
!! AGTTTTTCACTTTGGAAACT+TGG + chr1.4:74443140-74443159 None:intergenic 30.0%
!! TTGTCTCATAACAGTTTTAG+GGG - chr1.4:74442742-74442761 MS.gene34000:intron 30.0%
!! TTTTAGGGGAACAAAATCAT+GGG - chr1.4:74442756-74442775 MS.gene34000:intron 30.0%
!!! TGATATGAGTTGCTTTTTAG+AGG - chr1.4:74443662-74443681 MS.gene34000:CDS 30.0%
AACTCATATCAACATGAGGT+TGG + chr1.4:74443653-74443672 None:intergenic 35.0%
AAGCAACTCATATCAACATG+AGG + chr1.4:74443657-74443676 None:intergenic 35.0%
AATATTGAGAAGCTGGTGAA+GGG + chr1.4:74442303-74442322 None:intergenic 35.0%
ACTCATATCAACATGAGGTT+GGG + chr1.4:74443652-74443671 None:intergenic 35.0%
ATGAAGCAAATGCAAGGATA+AGG - chr1.4:74443436-74443455 MS.gene34000:CDS 35.0%
CAACATCATTAAGTTCTTGC+AGG - chr1.4:74442498-74442517 MS.gene34000:intron 35.0%
GCTGATTAATCATCATGTCA+TGG + chr1.4:74443629-74443648 None:intergenic 35.0%
GTCTAACGATTATGGTCAAT+GGG - chr1.4:74443104-74443123 MS.gene34000:intron 35.0%
TCTAACGATTATGGTCAATG+GGG - chr1.4:74443105-74443124 MS.gene34000:intron 35.0%
TGAAGCAAATGCAAGGATAA+GGG - chr1.4:74443437-74443456 MS.gene34000:CDS 35.0%
TGGTTTATGAAGCAAATGCA+AGG - chr1.4:74443430-74443449 MS.gene34000:CDS 35.0%
TGTCTAACGATTATGGTCAA+TGG - chr1.4:74443103-74443122 MS.gene34000:intron 35.0%
TGTGAATGGGAACATGAATT+GGG + chr1.4:74442213-74442232 None:intergenic 35.0%
TTAATCATCATGTCATGGTC+TGG + chr1.4:74443624-74443643 None:intergenic 35.0%
TTGCCAACAAGCAAATTTAG+TGG - chr1.4:74443563-74443582 MS.gene34000:CDS 35.0%
! AAAGGAGCATACCTAATTGA+TGG - chr1.4:74442811-74442830 MS.gene34000:intron 35.0%
! AAGGAGCATACCTAATTGAT+GGG - chr1.4:74442812-74442831 MS.gene34000:intron 35.0%
! ATAATTAGGTGCCCACATTT+TGG - chr1.4:74443067-74443086 MS.gene34000:intron 35.0%
! TGCACTCAAGTTTTTCACTT+TGG + chr1.4:74443148-74443167 None:intergenic 35.0%
! TGTAGTAGAGTTTGATAGCA+TGG + chr1.4:74442236-74442255 None:intergenic 35.0%
! TTTAGTGGCACTAATTTGCA+TGG - chr1.4:74443578-74443597 MS.gene34000:CDS 35.0%
! TTTGATAGCATGGTGTGAAT+GGG + chr1.4:74442226-74442245 None:intergenic 35.0%
!! ATGCATAGCTTTGATGTGTT+AGG + chr1.4:74442865-74442884 None:intergenic 35.0%
!!! GTTTTAGGGGAACAAAATCA+TGG - chr1.4:74442755-74442774 MS.gene34000:intron 35.0%
ACCATTGCTCATAGAGTCTT+TGG - chr1.4:74442469-74442488 MS.gene34000:CDS 40.0%
ACTGGTCATAGTCACAAACA+AGG + chr1.4:74442665-74442684 None:intergenic 40.0%
ATCATGCATGCCCATCAATT+AGG + chr1.4:74442825-74442844 None:intergenic 40.0%
ATGGGAACATGAATTGGGAT+TGG + chr1.4:74442208-74442227 None:intergenic 40.0%
CAAGGGCTAACAATAATTGG+TGG + chr1.4:74442364-74442383 None:intergenic 40.0%
CTCATATCAACATGAGGTTG+GGG + chr1.4:74443651-74443670 None:intergenic 40.0%
GAATATTGAGAAGCTGGTGA+AGG + chr1.4:74442304-74442323 None:intergenic 40.0%
GCACAAGGGCTAACAATAAT+TGG + chr1.4:74442367-74442386 None:intergenic 40.0%
GGATCAGTTGGAGGAAAATA+CGG + chr1.4:74442439-74442458 None:intergenic 40.0%
GTGTGAATGGGAACATGAAT+TGG + chr1.4:74442214-74442233 None:intergenic 40.0%
TCCAAAGACTCTATGAGCAA+TGG + chr1.4:74442473-74442492 None:intergenic 40.0%
! AAAGGTGTGGGAGATTGTAA+AGG + chr1.4:74442280-74442299 None:intergenic 40.0%
! GGGAACAAAATCATGGGTTT+TGG - chr1.4:74442762-74442781 MS.gene34000:intron 40.0%
! GTGCCACTAAATTTGCTTGT+TGG + chr1.4:74443569-74443588 None:intergenic 40.0%
! GTTTGATAGCATGGTGTGAA+TGG + chr1.4:74442227-74442246 None:intergenic 40.0%
! TGAATTGGGATTGGTAGATG+AGG + chr1.4:74442199-74442218 None:intergenic 40.0%
!! ACAACAATTTTGGGTCTGCT+TGG - chr1.4:74443685-74443704 MS.gene34000:CDS 40.0%
!! CAACAATTTTGGGTCTGCTT+GGG - chr1.4:74443686-74443705 MS.gene34000:CDS 40.0%
!! CATGGGTTTTGGTCAAAAAG+TGG - chr1.4:74442773-74442792 MS.gene34000:intron 40.0%
!! CTAACAATAATTGGTGGTGG+AGG + chr1.4:74442358-74442377 None:intergenic 40.0%
!! GGTTTTGGTCAAAAAGTGGA+TGG - chr1.4:74442777-74442796 MS.gene34000:intron 40.0%
AACTTGAGTGGATCAGTTGG+AGG + chr1.4:74442448-74442467 None:intergenic 45.0%
AAGAGAATGTCATGCCTCGA+AGG + chr1.4:74442697-74442716 None:intergenic 45.0%
AGGATAAGGGACCCAGTTTA+TGG - chr1.4:74443450-74443469 MS.gene34000:CDS 45.0%
ATAGTGGTTACACTATGGCC+AGG + chr1.4:74443028-74443047 None:intergenic 45.0%
GGAGGAGAATATTGAGAAGC+TGG + chr1.4:74442310-74442329 None:intergenic 45.0%
GGAGGTGAAGAAGATGAAGA+TGG + chr1.4:74442259-74442278 None:intergenic 45.0%
GGATAAGGGACCCAGTTTAT+GGG - chr1.4:74443451-74443470 MS.gene34000:CDS 45.0%
GGGCTAACAATAATTGGTGG+TGG + chr1.4:74442361-74442380 None:intergenic 45.0%
GTGAACTTGAGTGGATCAGT+TGG + chr1.4:74442451-74442470 None:intergenic 45.0%
TAGATGAGGAGGAGAAGTAG+TGG + chr1.4:74442185-74442204 None:intergenic 45.0%
TAGTGGTTACACTATGGCCA+GGG + chr1.4:74443027-74443046 None:intergenic 45.0%
TGAGCAATGGTGAACTTGAG+TGG + chr1.4:74442460-74442479 None:intergenic 45.0%
! ACTTTTCAACACACCTGCGA+CGG + chr1.4:74442405-74442424 None:intergenic 45.0%
! ATTGGGATTGGTAGATGAGG+AGG + chr1.4:74442196-74442215 None:intergenic 45.0%
!! ATAATTGGTGGTGGAGGAGA+TGG + chr1.4:74442352-74442371 None:intergenic 45.0%
AGAATCTTGCAGGCAGCACA+AGG + chr1.4:74442382-74442401 None:intergenic 50.0%
AGCAGATGCAGTAAGCAGCA+TGG - chr1.4:74443410-74443429 MS.gene34000:CDS 50.0%
AGTGGTTACACTATGGCCAG+GGG + chr1.4:74443026-74443045 None:intergenic 50.0%
CACCTGCACACCCATAAACT+GGG + chr1.4:74443464-74443483 None:intergenic 50.0%
CACTAGCTCAGCTTGTGCTT+TGG + chr1.4:74443535-74443554 None:intergenic 50.0%
CCAGTTCATTCACACCTTCG+AGG - chr1.4:74442680-74442699 MS.gene34000:intron 50.0%
CTTACTGCATCTGCTCTCTG+AGG + chr1.4:74443405-74443424 None:intergenic 50.0%
CTTTCTCTCTTTAGTCCCCC+TGG - chr1.4:74443007-74443026 MS.gene34000:intron 50.0%
GAATCTTGCAGGCAGCACAA+GGG + chr1.4:74442381-74442400 None:intergenic 50.0%
GGTGTGGGAGATTGTAAAGG+AGG + chr1.4:74442277-74442296 None:intergenic 50.0%
TGAGAAGCTGGTGAAGGGAA+AGG + chr1.4:74442298-74442317 None:intergenic 50.0%
! CCTCGAAGGTGTGAATGAAC+TGG + chr1.4:74442683-74442702 None:intergenic 50.0%
AGCTGGTGAAGGGAAAGGTG+TGG + chr1.4:74442293-74442312 None:intergenic 55.0%
GACCCAGTTTATGGGTGTGC+AGG - chr1.4:74443459-74443478 MS.gene34000:CDS 55.0%
GCACCTGCACACCCATAAAC+TGG + chr1.4:74443465-74443484 None:intergenic 55.0%
GTGGTTACACTATGGCCAGG+GGG + chr1.4:74443025-74443044 None:intergenic 55.0%
! GCTGGTGAAGGGAAAGGTGT+GGG + chr1.4:74442292-74442311 None:intergenic 55.0%
!! ATTGGTGGTGGAGGAGATGG+TGG + chr1.4:74442349-74442368 None:intergenic 55.0%
!! GCATCTGCTCTCTGAGGTTC+AGG + chr1.4:74443399-74443418 None:intergenic 55.0%
CCTGCAAGATTCTCCGTCGC+AGG - chr1.4:74442389-74442408 MS.gene34000:CDS 60.0%
CCTGCGACGGAGAATCTTGC+AGG + chr1.4:74442392-74442411 None:intergenic 60.0%
GGTCTGCTTGGGAGCCTCTT+TGG - chr1.4:74443697-74443716 MS.gene34000:CDS 60.0%
GGTGGTTGAGGAGCAGCAGA+GGG + chr1.4:74442331-74442350 None:intergenic 60.0%
TGGTGGTTGAGGAGCAGCAG+AGG + chr1.4:74442332-74442351 None:intergenic 60.0%
! GGTGGAGGAGATGGTGGTTG+AGG + chr1.4:74442343-74442362 None:intergenic 60.0%
GGTTGAGGAGCAGCAGAGGG+AGG + chr1.4:74442328-74442347 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74442163 74443725 74442163 ID=MS.gene34000
chr1.4 mRNA 74442163 74443725 74442163 ID=MS.gene34000.t1;Parent=MS.gene34000
chr1.4 exon 74443369 74443725 74443369 ID=MS.gene34000.t1.exon1;Parent=MS.gene34000.t1
chr1.4 CDS 74443369 74443725 74443369 ID=cds.MS.gene34000.t1;Parent=MS.gene34000.t1
chr1.4 exon 74442163 74442498 74442163 ID=MS.gene34000.t1.exon2;Parent=MS.gene34000.t1
chr1.4 CDS 74442163 74442498 74442163 ID=cds.MS.gene34000.t1;Parent=MS.gene34000.t1
Gene Sequence

>MS.gene34000

ATGGAACACAAAATGAAAACCACTACTTCTCCTCCTCATCTACCAATCCCAATTCATGTTCCCATTCACACCATGCTATCAAACTCTACTACACCATCTTCATCTTCTTCACCTCCTTTACAATCTCCCACACCTTTCCCTTCACCAGCTTCTCAATATTCTCCTCCCTCTGCTGCTCCTCAACCACCATCTCCTCCACCACCAATTATTGTTAGCCCTTGTGCTGCCTGCAAGATTCTCCGTCGCAGGTGTGTTGAAAAGTGTGTGTTAGCTCCGTATTTTCCTCCAACTGATCCACTCAAGTTCACCATTGCTCATAGAGTCTTTGGAGCTAGCAACATCATTAAGTTCTTGCAGGTAACATACATTCATAATTCACCAACAAGAAAAAATCATCATGTTACAAACCAATTTGCACATTACTAAAATATTTTTTTCAACATATTGGACATTTATATGTATGATCTTAAAACAAATTGGTGCGATTCTATTGTTGTTGCCTTGTTTGTGACTATGACCAGTTCATTCACACCTTCGAGGCATGACATTCTCTTGAAAAACAACAAAAAATTAGTTGAATTGTCTCATAACAGTTTTAGGGGAACAAAATCATGGGTTTTGGTCAAAAAGTGGATGGAAAAGATAACAAAAGGAGCATACCTAATTGATGGGCATGCATGATGTGTTGTTTTCTAACTTCCTAACACATCAAAGCTATGCATAATTCTCTCTTCTTTACATTCTACATCAACCAACCACTAACTTTTTATCTTTTACATGAAAAAAATAAAAGCTATGGACAAGTCAAAATAACATTTTGCATATTGCTTGTTTATCTTAATTTCTTTCTCTCTTTAGTCCCCCTGGCCATAGTGTAACCACTATATCTTATCACAAGTCAAAAATAATTAGGTGCCCACATTTTGGAATCTTTTCATTATGTCTAACGATTATGGTCAATGGGGCATGAAGTACCAAGTTTCCAAAGTGAAAAACTTGAGTGCATATCTTTAGTCTTCGTATTCTTCCTAACTTATAGTACATTTTATTTATAGTTATAATAAATTTAGAGAAAATCTTGGTAGTTATAATAAGTGAAAAATGGTACGAAAAGTACCTAAACAAATGCACATAAGTTTTGTCCATTTTCAAATCATGGCTTAATTTGTAATTAAGAGTTTTAATTAGTTAGTAAATAACAAAAATAAAATTCCATTAATTTTTCAGGAACTACCTGAACCTCAGAGAGCAGATGCAGTAAGCAGCATGGTTTATGAAGCAAATGCAAGGATAAGGGACCCAGTTTATGGGTGTGCAGGTGCAATATGTCAACTTCAAAAACAAGTTAGTGAGTTACAAACACAGCTAGCCAAAGCACAAGCTGAGCTAGTGAACATGCATTGCCAACAAGCAAATTTAGTGGCACTAATTTGCATGGAAATGAGTACTCAGTCATCACCAGACCATGACATGATGATTAATCAGCCCCAACCTCATGTTGATATGAGTTGCTTTTTAGAGGACAACAATTTTGGGTCTGCTTGGGAGCCTCTTTGGACATGA
Protein sequence

>MS.gene34000.t1

MEHKMKTTTSPPHLPIPIHVPIHTMLSNSTTPSSSSSPPLQSPTPFPSPASQYSPPSAAPQPPSPPPPIIVSPCAACKILRRRCVEKCVLAPYFPPTDPLKFTIAHRVFGASNIIKFLQELPEPQRADAVSSMVYEANARIRDPVYGCAGAICQLQKQVSELQTQLAKAQAELVNMHCQQANLVALICMEMSTQSSPDHDMMINQPQPHVDMSCFLEDNNFGSAWEPLWT