AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34460.t1 MTR_6g051425 28.477 302 190 7 65 344 19 316 1.91e-32 129
MS.gene34460.t1 MTR_5g015480 45.455 110 59 1 54 162 47 156 8.44e-23 99.4
MS.gene34460.t1 MTR_0558s0020 24.528 318 189 8 16 305 1 295 1.51e-22 99.4
MS.gene34460.t1 MTR_0402s0040 31.088 193 129 3 54 245 17 206 5.33e-21 97.8
MS.gene34460.t1 MTR_6g017200 38.760 129 74 2 31 158 30 154 1.40e-20 89.7
MS.gene34460.t1 MTR_2g070590 40.909 110 64 1 54 162 8 117 4.40e-20 87.0
MS.gene34460.t1 MTR_4g067290 35.032 157 90 5 27 180 27 174 5.40e-19 87.4
MS.gene34460.t1 MTR_4g067290 34.437 151 90 4 27 177 27 168 7.51e-19 87.0
MS.gene34460.t1 MTR_4g067290 35.032 157 90 5 27 180 47 194 8.74e-19 87.4
MS.gene34460.t1 MTR_1g057920 44.318 88 48 1 65 151 36 123 1.08e-18 83.2
MS.gene34460.t1 MTR_4g067290 34.437 151 90 4 27 177 47 188 1.31e-18 87.0
MS.gene34460.t1 MTR_4g067290 34.437 151 90 4 27 177 45 186 1.34e-18 87.0
MS.gene34460.t1 MTR_4g067290 35.135 148 84 4 43 180 89 234 1.62e-18 87.4
MS.gene34460.t1 MTR_8g446020 25.926 189 127 2 123 305 74 255 3.10e-18 85.9
MS.gene34460.t1 MTR_5g039760 28.571 196 112 3 65 259 12 180 1.18e-17 82.4
MS.gene34460.t1 MTR_2g098920 37.755 98 60 1 65 162 82 178 4.66e-16 79.7
MS.gene34460.t1 MTR_1g070355 26.250 240 159 7 69 300 80 309 1.20e-15 81.6
MS.gene34460.t1 MTR_6g090505 23.853 218 139 4 108 305 20 230 1.32e-15 80.1
MS.gene34460.t1 MTR_7g113010 37.288 118 59 2 57 173 451 554 4.08e-14 76.6
MS.gene34460.t1 MTR_7g061980 36.607 112 68 3 65 175 12 121 7.12e-14 69.3
MS.gene34460.t1 MTR_1g083640 21.941 237 146 6 68 276 56 281 2.80e-12 68.9
MS.gene34460.t1 MTR_3g006370 29.870 154 93 2 66 204 53 206 2.80e-12 67.8
MS.gene34460.t1 MTR_0137s0040 54.167 48 22 0 69 116 42 89 3.82e-12 64.3
MS.gene34460.t1 MTR_2g098880 36.111 108 61 2 65 167 63 167 5.70e-12 67.4
MS.gene34460.t1 MTR_2g098880 36.111 108 61 2 65 167 75 179 7.81e-12 67.0
MS.gene34460.t1 MTR_1g086270 32.500 80 54 0 360 439 1 80 2.87e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34460.t1 AT4G38180 31.962 632 406 6 13 623 6 634 4.51e-103 338
MS.gene34460.t1 AT4G15090 26.984 819 532 14 66 877 54 813 5.44e-90 303
MS.gene34460.t1 AT4G15090 26.984 819 532 14 66 877 54 813 5.44e-90 303
MS.gene34460.t1 AT4G15090 26.984 819 532 14 66 877 54 813 5.44e-90 303
MS.gene34460.t1 AT4G15090 26.984 819 532 14 66 877 54 813 5.44e-90 303
MS.gene34460.t1 AT4G15090 26.984 819 532 14 66 877 54 813 5.44e-90 303
MS.gene34460.t1 AT3G06250 30.498 623 375 10 66 685 193 760 4.14e-88 297
MS.gene34460.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.53e-12 70.1
MS.gene34460.t1 AT3G06250 30.498 623 375 10 66 685 193 760 4.14e-88 297
MS.gene34460.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.53e-12 70.1
MS.gene34460.t1 AT3G06250 30.498 623 375 10 66 685 193 760 4.14e-88 297
MS.gene34460.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.53e-12 70.1
MS.gene34460.t1 AT5G18960 29.841 630 373 13 66 685 214 784 1.02e-83 286
MS.gene34460.t1 AT5G18960 30.464 151 84 3 13 163 1 130 1.94e-12 71.6
MS.gene34460.t1 AT5G18960 29.841 630 373 13 66 685 214 784 1.02e-83 286
MS.gene34460.t1 AT5G18960 30.464 151 84 3 13 163 1 130 1.94e-12 71.6
MS.gene34460.t1 AT3G22170 26.630 721 456 9 2 698 16 687 2.50e-82 283
MS.gene34460.t1 AT3G22170 26.630 721 456 9 2 698 16 687 2.50e-82 283
MS.gene34460.t1 AT2G27110 30.941 627 393 13 65 689 51 639 2.37e-80 278
MS.gene34460.t1 AT2G27110 30.941 627 393 13 65 689 51 639 2.37e-80 278
MS.gene34460.t1 AT1G76320 28.943 615 413 6 69 680 3 596 3.27e-79 272
MS.gene34460.t1 AT1G76320 28.943 615 413 6 69 680 3 596 3.27e-79 272
MS.gene34460.t1 AT1G76320 28.943 615 413 6 69 680 3 596 4.43e-79 271
MS.gene34460.t1 AT1G76320 29.268 615 411 7 69 680 3 596 6.32e-79 270
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 2.47e-76 265
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 2.73e-76 265
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 2.73e-76 265
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 3.16e-76 265
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 3.16e-76 265
MS.gene34460.t1 AT2G32250 27.597 645 408 10 66 704 41 632 3.16e-76 265
MS.gene34460.t1 AT2G32250 27.570 642 406 10 69 704 3 591 3.53e-76 264
MS.gene34460.t1 AT2G32250 27.570 642 406 10 69 704 3 591 3.89e-76 264
MS.gene34460.t1 AT2G27110 32.389 494 314 8 197 689 20 494 2.48e-73 255
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 24 637 7.65e-71 249
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 24 637 8.02e-71 249
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 24 637 8.02e-71 249
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 38 651 1.24e-70 249
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 24 637 1.59e-70 249
MS.gene34460.t1 AT4G19990 28.659 656 408 11 66 703 24 637 1.59e-70 249
MS.gene34460.t1 AT4G38170 28.252 492 323 6 230 719 10 473 1.76e-70 243
MS.gene34460.t1 AT1G52520 27.889 649 405 18 64 685 86 698 8.96e-65 231
MS.gene34460.t1 AT4G19990 28.065 620 386 11 102 703 3 580 1.00e-62 225
MS.gene34460.t1 AT1G80010 26.976 582 383 14 66 623 98 661 6.26e-58 212
MS.gene34460.t1 AT1G10240 24.077 623 436 14 60 666 45 646 1.65e-46 178
MS.gene34460.t1 AT1G10240 24.077 623 436 14 60 666 45 646 1.65e-46 178
MS.gene34460.t1 AT5G28530 23.934 610 378 15 60 622 50 620 7.75e-41 161
MS.gene34460.t1 AT5G28530 23.732 611 374 17 60 622 50 616 1.50e-39 157
MS.gene34460.t1 AT5G28530 23.732 611 374 17 60 622 50 616 1.50e-39 157
MS.gene34460.t1 AT5G28530 22.590 332 210 6 312 622 252 557 1.29e-20 97.8
MS.gene34460.t1 AT5G28530 22.222 333 206 8 312 622 252 553 2.49e-19 93.6
MS.gene34460.t1 AT3G07500 42.593 108 61 1 65 172 35 141 3.54e-18 84.7
MS.gene34460.t1 AT3G59470 39.796 98 58 1 65 162 72 168 8.69e-18 84.3
MS.gene34460.t1 AT3G59470 39.796 98 58 1 65 162 72 168 8.69e-18 84.3
MS.gene34460.t1 AT3G59470 39.796 98 58 1 65 162 84 180 9.67e-18 84.7
MS.gene34460.t1 AT2G43280 29.221 154 94 3 66 204 25 178 1.45e-13 70.9
MS.gene34460.t1 AT4G12850 32.653 98 63 1 65 162 13 107 3.71e-13 68.2
MS.gene34460.t1 AT4G12850 32.323 99 64 1 65 163 13 108 5.63e-12 64.7
MS.gene34460.t1 AT4G12850 32.653 98 63 1 65 162 13 107 1.41e-11 64.7

Find 174 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCGGACTGCAACAATTT+TGG 0.148412 1.2:+75678437 None:intergenic
GGTTGACTCATATCATAATT+TGG 0.153971 1.2:-75677966 MS.gene34460:CDS
AGGATTCTGAATGAGGATTT+TGG 0.176822 1.2:+75675528 None:intergenic
CAGCTTGCTACAACCATCTT+AGG 0.224643 1.2:+75676615 None:intergenic
CATGTATACCGGTCTGTTTC+AGG 0.224714 1.2:+75677425 None:intergenic
CGGACTCATGGTATGTGTTT+TGG 0.233173 1.2:+75675711 None:intergenic
AGACCATCGAATCATTTAAT+TGG 0.243258 1.2:-75677547 MS.gene34460:CDS
AGTATGACGAATACAAAATT+AGG 0.255620 1.2:-75677920 MS.gene34460:CDS
ACTTAAAGCTGAACTAGATT+TGG 0.264524 1.2:+75677940 None:intergenic
GATTATGTTGTGCTTCAAAA+TGG 0.267872 1.2:+75678068 None:intergenic
GTGGTAGATTACGACCATTT+TGG 0.280325 1.2:-75677692 MS.gene34460:CDS
ATGCAAGGTTGGGTAATTTC+AGG 0.281403 1.2:-75676668 MS.gene34460:CDS
TTAGAAAAGATTGGATTAAT+AGG 0.287407 1.2:-75678261 MS.gene34460:CDS
ACTTTCATTGGCGCAATGTC+TGG 0.289475 1.2:-75677515 MS.gene34460:CDS
CTTACCTGCAAAGGGAGATT+TGG 0.310345 1.2:+75676189 None:intergenic
CCTCCACTATCCTTTCAAAA+TGG 0.316329 1.2:+75677075 None:intergenic
TTCTGTGAGGGAAAGAGATA+TGG 0.326936 1.2:-75677846 MS.gene34460:CDS
AGTAAATTCTTCCTCGTCCT+TGG 0.329251 1.2:+75677304 None:intergenic
AAGATAATTGTCATAATTCA+TGG 0.332546 1.2:+75677871 None:intergenic
ACTTGGAACATCCAATAGTT+TGG 0.334138 1.2:-75678473 MS.gene34460:CDS
GTAAATTCTTCCTCGTCCTT+GGG 0.340043 1.2:+75677305 None:intergenic
CACATTCAAGTCCAAACTAT+TGG 0.341769 1.2:+75678462 None:intergenic
CATGCATTGAACCACTTCAT+TGG 0.347987 1.2:+75675938 None:intergenic
CCGAACGTTCTTCACGCTAA+TGG 0.356305 1.2:-75678421 MS.gene34460:CDS
AGCATCTTGTTCTGTGATAA+TGG 0.360975 1.2:+75677478 None:intergenic
AACAACATGTTAGAATTGAT+TGG 0.361206 1.2:+75678031 None:intergenic
TTTCTGGGCTGATGACAATA+TGG 0.363592 1.2:-75677714 MS.gene34460:CDS
GTTGACTCATATCATAATTT+GGG 0.366463 1.2:-75677965 MS.gene34460:CDS
TACATGGTATGGTGGTTTCT+AGG 0.366745 1.2:-75678231 MS.gene34460:CDS
TCAACCAAATCTCCCTTTGC+AGG 0.367806 1.2:-75676193 MS.gene34460:intron
CTTAAAGCTGAACTAGATTT+GGG 0.370982 1.2:+75677941 None:intergenic
GGCTTTGTCAACACAGTGTT+CGG 0.372860 1.2:-75678361 MS.gene34460:CDS
AATCCTTTGCAAATGATCTA+AGG 0.380215 1.2:-75677343 MS.gene34460:CDS
GGTTCAATGTTAGAAAAGAT+TGG 0.384400 1.2:-75678270 MS.gene34460:CDS
AGGAGAAGCTGGACGTTTGA+TGG 0.385911 1.2:-75677819 MS.gene34460:CDS
CCACAGACTTGTCAAGTTAT+CGG 0.386090 1.2:-75676577 MS.gene34460:CDS
GATGTCAAAGGCTTTCATTT+AGG 0.388977 1.2:-75677164 MS.gene34460:CDS
GTATAGAGAATGGGAGAAAT+GGG 0.389543 1.2:-75677219 MS.gene34460:CDS
GGGATGTCTTCCAAAGCTAA+TGG 0.397500 1.2:-75677012 MS.gene34460:CDS
TGAAATATTTCAAATGCTCT+TGG 0.400052 1.2:+75676948 None:intergenic
TGTATAGAGAATGGGAGAAA+TGG 0.401167 1.2:-75677220 MS.gene34460:CDS
TTTGGTTGAGATGAAACATA+TGG 0.404147 1.2:+75676207 None:intergenic
GGAAGAATTTACTCATGCTT+GGG 0.404564 1.2:-75677294 MS.gene34460:CDS
AGAAGTTTGGCGGTTGGAAC+AGG 0.408816 1.2:+75675871 None:intergenic
TGTTCTGTGATAATGGCCTT+AGG 0.409137 1.2:+75677485 None:intergenic
TGTTGGTTAGAATAGAAGTT+TGG 0.411083 1.2:+75675858 None:intergenic
AACAAACAACATCCCCAAAA+TGG 0.411804 1.2:+75677678 None:intergenic
GAGTATAAAGTGAATACATT+TGG 0.412707 1.2:-75676861 MS.gene34460:CDS
TGGCTGTTTCGAACTTTCAT+TGG 0.414516 1.2:-75677527 MS.gene34460:CDS
TGTATTATCTGAAGAATTGA+AGG 0.418492 1.2:+75676815 None:intergenic
ATCTCAACCATTCATTTCGC+TGG 0.423879 1.2:+75677246 None:intergenic
ACCACATTACCCATTAGCTT+TGG 0.425279 1.2:+75677002 None:intergenic
AACATTGAACCCCATCAATC+TGG 0.425557 1.2:+75678282 None:intergenic
ACAACATGTTAGAATTGATT+GGG 0.429453 1.2:+75678032 None:intergenic
AGTCAGAACTTCGGTATCTA+TGG 0.430265 1.2:+75675489 None:intergenic
TCTTAAACATCTCAGCCATT+TGG 0.431357 1.2:-75677378 MS.gene34460:CDS
ATTCGTCATCTTTAACCAAA+TGG 0.433155 1.2:+75677363 None:intergenic
TGGACATGCGACTGAGGATC+AGG 0.436653 1.2:-75676382 MS.gene34460:CDS
GGAGACGAGAACTGGATGTT+TGG 0.438770 1.2:-75678137 MS.gene34460:CDS
CATCGGAAGGAGACGAGAAC+TGG 0.445746 1.2:-75678145 MS.gene34460:CDS
GAGAAATGGGCTGTCTGCTT+TGG 0.447459 1.2:-75677206 MS.gene34460:CDS
TAACAAGTATGCCAGATTGA+TGG 0.451658 1.2:-75678293 MS.gene34460:CDS
AATGGCTCTCAAACTCAATC+TGG 0.454597 1.2:-75678403 MS.gene34460:CDS
ATAATCAAACTGATTCAGAT+AGG 0.455395 1.2:-75676287 MS.gene34460:CDS
AGGAAGAATTTACTCATGCT+TGG 0.458452 1.2:-75677295 MS.gene34460:CDS
AACAAGTATGCCAGATTGAT+GGG 0.458593 1.2:-75678292 MS.gene34460:CDS
TGATGATCTAACACAAGATC+AGG 0.463119 1.2:+75675906 None:intergenic
TCCTCAGTCGCATGTCCATT+CGG 0.464228 1.2:+75676387 None:intergenic
GTCAACACAGTGTTCGGAAT+CGG 0.464277 1.2:-75678355 MS.gene34460:CDS
GGATGTCTTCCAAAGCTAAT+GGG 0.465360 1.2:-75677011 MS.gene34460:CDS
AGAAGCTAGTGATGAAATGA+AGG 0.466016 1.2:-75677033 MS.gene34460:CDS
ATCTACATGAATACTATTTG+AGG 0.467176 1.2:+75676440 None:intergenic
GAGATGGATGTATAGAGAAT+GGG 0.471472 1.2:-75677228 MS.gene34460:CDS
TGTTGCATCTGATTATTGCT+TGG 0.479286 1.2:+75675686 None:intergenic
TGATAAAAGCTTACCTGCAA+AGG 0.481590 1.2:+75676180 None:intergenic
TGCAAGGTTGGGTAATTTCA+GGG 0.481977 1.2:-75676667 MS.gene34460:CDS
CAGACCGGTATACATGTGTT+TGG 0.482825 1.2:-75677418 MS.gene34460:CDS
CAGACAACAGTATTTGAAGA+AGG 0.483624 1.2:+75676249 None:intergenic
AGCTTGCTACAACCATCTTA+GGG 0.484144 1.2:+75676616 None:intergenic
GAGGCAACTTGACGAAGTTA+TGG 0.484472 1.2:-75676514 MS.gene34460:CDS
AGGGAAAGAGATATGGGTAA+AGG 0.486429 1.2:-75677839 MS.gene34460:CDS
AATTGAACTACATCAAGGTC+AGG 0.492101 1.2:+75677104 None:intergenic
AATTCAAGATAACCCTAAGA+TGG 0.492105 1.2:-75676628 MS.gene34460:CDS
CTCGATATATCTTGAAGAGA+TGG 0.495398 1.2:-75676698 MS.gene34460:CDS
AATTGACAGGACCGCTACAC+TGG 0.496426 1.2:-75676334 MS.gene34460:CDS
AAGCAATAATCAGATGCAAC+AGG 0.498238 1.2:-75675684 MS.gene34460:intron
CTTTCATTGGCGCAATGTCT+GGG 0.499304 1.2:-75677514 MS.gene34460:CDS
ATGGTCACACAGCAAAATAC+AGG 0.502031 1.2:+75675508 None:intergenic
TCTGGTAATGCATCAGCAAA+TGG 0.509074 1.2:-75678385 MS.gene34460:CDS
TAGGAGTAAGGTACATGGTA+TGG 0.509545 1.2:-75678242 MS.gene34460:CDS
ACGATATCCCTCTCTGGAGC+AGG 0.509622 1.2:+75678201 None:intergenic
GATGGTGATCATAACAACTT+AGG 0.510043 1.2:-75678526 MS.gene34460:CDS
GATAAAAGCTTACCTGCAAA+GGG 0.510969 1.2:+75676181 None:intergenic
CCATTAGCGTGAAGAACGTT+CGG 0.511332 1.2:+75678421 None:intergenic
ATTATGTTGTGCTTCAAAAT+GGG 0.511977 1.2:+75678069 None:intergenic
GTCATCAACATCGAGCTGTA+TGG 0.515154 1.2:+75677751 None:intergenic
TGAGATGAAACATATGGAGA+TGG 0.517004 1.2:+75676213 None:intergenic
GAGATGAAACATATGGAGAT+GGG 0.517572 1.2:+75676214 None:intergenic
ATGGACAAAAGATGCAAGGT+TGG 0.517774 1.2:-75676679 MS.gene34460:CDS
TAGAATAGAAGTTTGGCGGT+TGG 0.518493 1.2:+75675865 None:intergenic
AAAATACAGGATTCTGAATG+AGG 0.519697 1.2:+75675521 None:intergenic
ATCATTACTCGTCAACAAGA+TGG 0.520020 1.2:-75678106 MS.gene34460:CDS
TGAGATGGATGTATAGAGAA+TGG 0.522848 1.2:-75677229 MS.gene34460:CDS
GAAAATGTTGATGTTGGACT+TGG 0.524439 1.2:-75678490 MS.gene34460:CDS
TTTCCTACGATATCCCTCTC+TGG 0.525239 1.2:+75678195 None:intergenic
TATTGCTTGGCACGGACTCA+TGG 0.526252 1.2:+75675699 None:intergenic
GGAGAAGCTGGACGTTTGAT+GGG 0.526650 1.2:-75677818 MS.gene34460:CDS
TGGTTAGAATAGAAGTTTGG+CGG 0.528921 1.2:+75675861 None:intergenic
CATGTAGAAAATGTTGATGT+TGG 0.531700 1.2:-75678496 MS.gene34460:CDS
GATATGGGTAAAGGAGAAGC+TGG 0.535036 1.2:-75677830 MS.gene34460:CDS
AGATTGGATTAATAGGAGTA+AGG 0.541743 1.2:-75678254 MS.gene34460:CDS
GATTATAATTCACCGTTGGT+TGG 0.543940 1.2:+75676304 None:intergenic
CAATAGTTTGGACTTGAATG+TGG 0.544732 1.2:-75678461 MS.gene34460:CDS
TGGACAAAAGATGCAAGGTT+GGG 0.547513 1.2:-75676678 MS.gene34460:CDS
TTGCCTTTCTCAACTATCGC+AGG 0.548639 1.2:-75675826 MS.gene34460:intron
CAGCCAATTAAATGATTCGA+TGG 0.552432 1.2:+75677544 None:intergenic
TTGTGTTGCTCAAGCTGTGG+AGG 0.559431 1.2:-75677987 MS.gene34460:CDS
GTTTGATTATAATTCACCGT+TGG 0.560586 1.2:+75676300 None:intergenic
ATAAACTCCAGCGAAATGAA+TGG 0.563219 1.2:-75677253 MS.gene34460:CDS
GTCAACCAGCACAAAAGAGA+TGG 0.564837 1.2:-75676894 MS.gene34460:CDS
CAATTATCTTCATTCTGTGA+GGG 0.569801 1.2:-75677858 MS.gene34460:CDS
TAAATTCTTCCTCGTCCTTG+GGG 0.571062 1.2:+75677306 None:intergenic
TAAGAAATTGAACTACATCA+AGG 0.574535 1.2:+75677099 None:intergenic
AGAATTTACTCATGCTTGGG+GGG 0.575196 1.2:-75677291 MS.gene34460:CDS
AAAGGCAAGGAGAACTCTGT+TGG 0.577643 1.2:+75675841 None:intergenic
GAAGAATTTACTCATGCTTG+GGG 0.577829 1.2:-75677293 MS.gene34460:CDS
TCTGTGAGGGAAAGAGATAT+GGG 0.578639 1.2:-75677845 MS.gene34460:CDS
CACAGTGTTCGGAATCGGTA+CGG 0.578865 1.2:-75678350 MS.gene34460:CDS
TCCAAAGCTAATGGGTAATG+TGG 0.580465 1.2:-75677003 MS.gene34460:CDS
AATCTTGATATTGAAAAGTG+AGG 0.582977 1.2:-75676478 MS.gene34460:CDS
ACAGGACCGCTACACTGGAT+AGG 0.587540 1.2:-75676329 MS.gene34460:CDS
AAATTTACCTGCTCCAGAGA+GGG 0.588352 1.2:-75678208 MS.gene34460:CDS
AAGAGCTTCTGAATCTGTGG+AGG 0.588474 1.2:-75676553 MS.gene34460:CDS
TACTGTGAAGAAACATCGGA+AGG 0.591189 1.2:-75678158 MS.gene34460:CDS
AGCGAAATGAATGGTTGAGA+TGG 0.591609 1.2:-75677244 MS.gene34460:CDS
CCATTCGGAAAGATCACCGC+AGG 0.593497 1.2:+75676402 None:intergenic
GAGTAAGCGAAGAAATTGAC+AGG 0.593964 1.2:-75676347 MS.gene34460:CDS
ACACCTGCGATAGTTGAGAA+AGG 0.595685 1.2:+75675823 None:intergenic
GGCAAGAGCTTCTGAATCTG+TGG 0.597094 1.2:-75676556 MS.gene34460:CDS
GAGTAAGGTACATGGTATGG+TGG 0.599166 1.2:-75678239 MS.gene34460:CDS
TCTGCCAAACACATGTATAC+CGG 0.603544 1.2:+75677414 None:intergenic
CATCTGATTATTGCTTGGCA+CGG 0.603767 1.2:+75675691 None:intergenic
TATATATGACCCCAAGGACG+AGG 0.604602 1.2:-75677315 MS.gene34460:CDS
ACTCCTTAGATCATTTGCAA+AGG 0.605967 1.2:+75677340 None:intergenic
TCCGAATGGACATGCGACTG+AGG 0.607571 1.2:-75676388 MS.gene34460:CDS
ACTGGATAGGTGCCAACCAA+CGG 0.611215 1.2:-75676316 MS.gene34460:CDS
ACAATTATCTTCATTCTGTG+AGG 0.613083 1.2:-75677859 MS.gene34460:CDS
AGATGAAACATATGGAGATG+GGG 0.614637 1.2:+75676215 None:intergenic
TGTATGAAGTTTGAGCATGT+AGG 0.618522 1.2:-75676780 MS.gene34460:CDS
CTCTTCAAGATATATCGAGA+TGG 0.620754 1.2:+75676702 None:intergenic
AAGAATTTACTCATGCTTGG+GGG 0.622092 1.2:-75677292 MS.gene34460:CDS
TCAACAAGATGGAAAATACC+AGG 0.623065 1.2:-75678095 MS.gene34460:CDS
CCTGCGGTGATCTTTCCGAA+TGG 0.627373 1.2:-75676402 MS.gene34460:CDS
GTGCTTCAAAATGGGTAACC+TGG 0.628259 1.2:+75678077 None:intergenic
GATGAAACATATGGAGATGG+GGG 0.631905 1.2:+75676216 None:intergenic
AGAGATGGACAAAAGATGCA+AGG 0.632488 1.2:-75676683 MS.gene34460:CDS
TGCTTCAGAAAGTGAGCCTG+CGG 0.635679 1.2:-75676418 MS.gene34460:CDS
CAACTTGACGAAGTTATGGA+AGG 0.645660 1.2:-75676510 MS.gene34460:CDS
GAAGCTAGTGATGAAATGAA+GGG 0.646836 1.2:-75677032 MS.gene34460:CDS
CCGATAACTTGACAAGTCTG+TGG 0.655057 1.2:+75676577 None:intergenic
GAGTTGTATATATGACCCCA+AGG 0.660099 1.2:-75677321 MS.gene34460:CDS
ATTAATAGGAGTAAGGTACA+TGG 0.662022 1.2:-75678247 MS.gene34460:CDS
TGCGATAGTTGAGAAAGGCA+AGG 0.663759 1.2:+75675828 None:intergenic
AGAGCTTCTGAATCTGTGGA+GGG 0.666121 1.2:-75676552 MS.gene34460:CDS
GAAATTTACCTGCTCCAGAG+AGG 0.668278 1.2:-75678209 MS.gene34460:CDS
CTGGGCTGATGACAATATGG+TGG 0.677851 1.2:-75677711 MS.gene34460:CDS
GGTAATGCATCAGCAAATGG+AGG 0.677979 1.2:-75678382 MS.gene34460:CDS
GTTATGCAGAGTCAGAACTT+CGG 0.680514 1.2:+75675480 None:intergenic
TTGGCACCTATCCAGTGTAG+CGG 0.682153 1.2:+75676323 None:intergenic
ACAAGTATGCCAGATTGATG+GGG 0.683870 1.2:-75678291 MS.gene34460:CDS
AGGGTTATCAGTTTGCTGTG+AGG 0.684882 1.2:-75676533 MS.gene34460:CDS
ATGAAACATATGGAGATGGG+GGG 0.693657 1.2:+75676217 None:intergenic
GCTCCAGAGAGGGATATCGT+AGG 0.703657 1.2:-75678198 MS.gene34460:CDS
AATCATCGAAGCAATCAATG+TGG 0.773569 1.2:-75677453 MS.gene34460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAAAAAATAAAGAGAA+CGG - chr1.2:75678000-75678019 MS.gene34460:CDS 15.0%
!! TCATTTATTTGATTGATATA+TGG - chr1.2:75677850-75677869 MS.gene34460:CDS 15.0%
!! AAGATAATTGTCATAATTCA+TGG + chr1.2:75676142-75676161 None:intergenic 20.0%
!! TATAAAAAAAACCTAATGAC+TGG + chr1.2:75678221-75678240 None:intergenic 20.0%
!! TTAGAAAAGATTGGATTAAT+AGG - chr1.2:75675749-75675768 MS.gene34460:intron 20.0%
!!! AATTTCTTAACCATTTTGAA+AGG - chr1.2:75676925-75676944 MS.gene34460:CDS 20.0%
!!! ATATCATGAATATTTTACTC+AGG - chr1.2:75678266-75678285 MS.gene34460:CDS 20.0%
!!! ATTTTCTCATTTGTTTTACA+GGG - chr1.2:75678032-75678051 MS.gene34460:CDS 20.0%
! AACAACATGTTAGAATTGAT+TGG + chr1.2:75675982-75676001 None:intergenic 25.0%
! AATCTTGATATTGAAAAGTG+AGG - chr1.2:75677532-75677551 MS.gene34460:CDS 25.0%
! ACAACATGTTAGAATTGATT+GGG + chr1.2:75675981-75676000 None:intergenic 25.0%
! ACAGGTAAACATTTACTTAA+TGG - chr1.2:75678344-75678363 MS.gene34460:CDS 25.0%
! AGTATGACGAATACAAAATT+AGG - chr1.2:75676090-75676109 MS.gene34460:intron 25.0%
! ATCTACATGAATACTATTTG+AGG + chr1.2:75677573-75677592 None:intergenic 25.0%
! ATTATGTTGTGCTTCAAAAT+GGG + chr1.2:75675944-75675963 None:intergenic 25.0%
! GAGTATAAAGTGAATACATT+TGG - chr1.2:75677149-75677168 MS.gene34460:CDS 25.0%
! TAAGAAATTGAACTACATCA+AGG + chr1.2:75676914-75676933 None:intergenic 25.0%
! TGAAATATTTCAAATGCTCT+TGG + chr1.2:75677065-75677084 None:intergenic 25.0%
! TGTATTATCTGAAGAATTGA+AGG + chr1.2:75677198-75677217 None:intergenic 25.0%
!! ACAAGATAACAAATCTTTTC+TGG - chr1.2:75676280-75676299 MS.gene34460:CDS 25.0%
!! ATAATCAAACTGATTCAGAT+AGG - chr1.2:75677723-75677742 MS.gene34460:CDS 25.0%
!! ATTTTACTGTGAAGAAACAT+CGG - chr1.2:75675848-75675867 MS.gene34460:CDS 25.0%
!! CAAGATAACAAATCTTTTCT+GGG - chr1.2:75676281-75676300 MS.gene34460:CDS 25.0%
!! GTTGACTCATATCATAATTT+GGG - chr1.2:75676045-75676064 MS.gene34460:intron 25.0%
!! TGTTAATTTTTCCAGTCATT+AGG - chr1.2:75678207-75678226 MS.gene34460:CDS 25.0%
!!! AGATTTTTTGTAGATGTCAA+AGG - chr1.2:75676834-75676853 MS.gene34460:CDS 25.0%
!!! GATTTTCTCATTTGTTTTAC+AGG - chr1.2:75678031-75678050 MS.gene34460:CDS 25.0%
AAAATACAGGATTCTGAATG+AGG + chr1.2:75678492-75678511 None:intergenic 30.0%
AATCCTTTGCAAATGATCTA+AGG - chr1.2:75676667-75676686 MS.gene34460:CDS 30.0%
AATTCAAGATAACCCTAAGA+TGG - chr1.2:75677382-75677401 MS.gene34460:CDS 30.0%
ACAATTATCTTCATTCTGTG+AGG - chr1.2:75676151-75676170 MS.gene34460:intron 30.0%
ACACCTAAAAACTGTACAAA+TGG + chr1.2:75676393-75676412 None:intergenic 30.0%
ACTTAAAGCTGAACTAGATT+TGG + chr1.2:75676073-75676092 None:intergenic 30.0%
AGACCATCGAATCATTTAAT+TGG - chr1.2:75676463-75676482 MS.gene34460:CDS 30.0%
AGATTGGATTAATAGGAGTA+AGG - chr1.2:75675756-75675775 MS.gene34460:intron 30.0%
ATTAATAGGAGTAAGGTACA+TGG - chr1.2:75675763-75675782 MS.gene34460:intron 30.0%
ATTCGTCATCTTTAACCAAA+TGG + chr1.2:75676650-75676669 None:intergenic 30.0%
CAATTATCTTCATTCTGTGA+GGG - chr1.2:75676152-75676171 MS.gene34460:intron 30.0%
CATGTAGAAAATGTTGATGT+TGG - chr1.2:75675514-75675533 MS.gene34460:CDS 30.0%
CTTAAAGCTGAACTAGATTT+GGG + chr1.2:75676072-75676091 None:intergenic 30.0%
GATTATGTTGTGCTTCAAAA+TGG + chr1.2:75675945-75675964 None:intergenic 30.0%
GCATATTTGATATAGAGATC+AGG - chr1.2:75677900-75677919 MS.gene34460:CDS 30.0%
GGTTCAATGTTAGAAAAGAT+TGG - chr1.2:75675740-75675759 MS.gene34460:intron 30.0%
GTTTGATTATAATTCACCGT+TGG + chr1.2:75677713-75677732 None:intergenic 30.0%
TGTATGGCATAAAAAAATGC+TGG + chr1.2:75676246-75676265 None:intergenic 30.0%
TTTGGTTGAGATGAAACATA+TGG + chr1.2:75677806-75677825 None:intergenic 30.0%
! GGTTGACTCATATCATAATT+TGG - chr1.2:75676044-75676063 MS.gene34460:intron 30.0%
! GTTTTATGCTCGTTAACTTT+TGG - chr1.2:75677955-75677974 MS.gene34460:CDS 30.0%
! TATCAAGAACTTTTAGTGCA+TGG + chr1.2:75677268-75677287 None:intergenic 30.0%
! TGTTGGTTAGAATAGAAGTT+TGG + chr1.2:75678155-75678174 None:intergenic 30.0%
!! CTTTGTGTATATTTGCTGTA+GGG - chr1.2:75678444-75678463 MS.gene34460:CDS 30.0%
!! TAACCATTTTGAAAGGATAG+TGG - chr1.2:75676932-75676951 MS.gene34460:CDS 30.0%
!! TGGTATGTGTTTTGGATTAT+AGG + chr1.2:75678294-75678313 None:intergenic 30.0%
!!! GTTTGATGGGTTATTTTCAA+AGG - chr1.2:75676205-75676224 MS.gene34460:CDS 30.0%
!!! TTTGTTTTACAGGGCTTATT+TGG - chr1.2:75678041-75678060 MS.gene34460:CDS 30.0%
AACAAACAACATCCCCAAAA+TGG + chr1.2:75676335-75676354 None:intergenic 35.0%
AACAAGTATGCCAGATTGAT+GGG - chr1.2:75675718-75675737 MS.gene34460:CDS 35.0%
AAGAATTTACTCATGCTTGG+GGG - chr1.2:75676718-75676737 MS.gene34460:CDS 35.0%
AAGCAATAATCAGATGCAAC+AGG - chr1.2:75678326-75678345 MS.gene34460:CDS 35.0%
AATCATCGAAGCAATCAATG+TGG - chr1.2:75676557-75676576 MS.gene34460:CDS 35.0%
AATTGAACTACATCAAGGTC+AGG + chr1.2:75676909-75676928 None:intergenic 35.0%
ACTCCTTAGATCATTTGCAA+AGG + chr1.2:75676673-75676692 None:intergenic 35.0%
ACTTGGAACATCCAATAGTT+TGG - chr1.2:75675537-75675556 MS.gene34460:CDS 35.0%
AGAAGCTAGTGATGAAATGA+AGG - chr1.2:75676977-75676996 MS.gene34460:CDS 35.0%
AGATGAAACATATGGAGATG+GGG + chr1.2:75677798-75677817 None:intergenic 35.0%
AGCATCTTGTTCTGTGATAA+TGG + chr1.2:75676535-75676554 None:intergenic 35.0%
AGGAAGAATTTACTCATGCT+TGG - chr1.2:75676715-75676734 MS.gene34460:CDS 35.0%
ATAAACTCCAGCGAAATGAA+TGG - chr1.2:75676757-75676776 MS.gene34460:CDS 35.0%
ATCATTACTCGTCAACAAGA+TGG - chr1.2:75675904-75675923 MS.gene34460:CDS 35.0%
CAATAGTTTGGACTTGAATG+TGG - chr1.2:75675549-75675568 MS.gene34460:intron 35.0%
CACATTCAAGTCCAAACTAT+TGG + chr1.2:75675551-75675570 None:intergenic 35.0%
CAGACAACAGTATTTGAAGA+AGG + chr1.2:75677764-75677783 None:intergenic 35.0%
CAGCCAATTAAATGATTCGA+TGG + chr1.2:75676469-75676488 None:intergenic 35.0%
CTCGATATATCTTGAAGAGA+TGG - chr1.2:75677312-75677331 MS.gene34460:CDS 35.0%
CTCTTCAAGATATATCGAGA+TGG + chr1.2:75677311-75677330 None:intergenic 35.0%
GAAAATGTTGATGTTGGACT+TGG - chr1.2:75675520-75675539 MS.gene34460:CDS 35.0%
GAAGAATTTACTCATGCTTG+GGG - chr1.2:75676717-75676736 MS.gene34460:CDS 35.0%
GAAGCTAGTGATGAAATGAA+GGG - chr1.2:75676978-75676997 MS.gene34460:CDS 35.0%
GAGATGAAACATATGGAGAT+GGG + chr1.2:75677799-75677818 None:intergenic 35.0%
GAGATGGATGTATAGAGAAT+GGG - chr1.2:75676782-75676801 MS.gene34460:CDS 35.0%
GATAAAAGCTTACCTGCAAA+GGG + chr1.2:75677832-75677851 None:intergenic 35.0%
GATGGTGATCATAACAACTT+AGG - chr1.2:75675484-75675503 MS.gene34460:CDS 35.0%
GATGTCAAAGGCTTTCATTT+AGG - chr1.2:75676846-75676865 MS.gene34460:CDS 35.0%
GATTATAATTCACCGTTGGT+TGG + chr1.2:75677709-75677728 None:intergenic 35.0%
GGAAGAATTTACTCATGCTT+GGG - chr1.2:75676716-75676735 MS.gene34460:CDS 35.0%
GTATAGAGAATGGGAGAAAT+GGG - chr1.2:75676791-75676810 MS.gene34460:CDS 35.0%
TAACAAGTATGCCAGATTGA+TGG - chr1.2:75675717-75675736 MS.gene34460:CDS 35.0%
TCAACAAGATGGAAAATACC+AGG - chr1.2:75675915-75675934 MS.gene34460:CDS 35.0%
TCTTAAACATCTCAGCCATT+TGG - chr1.2:75676632-75676651 MS.gene34460:CDS 35.0%
TGAGATGAAACATATGGAGA+TGG + chr1.2:75677800-75677819 None:intergenic 35.0%
TGAGATGGATGTATAGAGAA+TGG - chr1.2:75676781-75676800 MS.gene34460:CDS 35.0%
TGATAAAAGCTTACCTGCAA+AGG + chr1.2:75677833-75677852 None:intergenic 35.0%
TGATGATCTAACACAAGATC+AGG + chr1.2:75678107-75678126 None:intergenic 35.0%
TGGTTAGAATAGAAGTTTGG+CGG + chr1.2:75678152-75678171 None:intergenic 35.0%
TGTATAGAGAATGGGAGAAA+TGG - chr1.2:75676790-75676809 MS.gene34460:CDS 35.0%
TGTATGAAGTTTGAGCATGT+AGG - chr1.2:75677230-75677249 MS.gene34460:CDS 35.0%
TGTTGCATCTGATTATTGCT+TGG + chr1.2:75678327-75678346 None:intergenic 35.0%
! AATATCCATCTCTTTTGTGC+TGG + chr1.2:75677124-75677143 None:intergenic 35.0%
! AGGATTCTGAATGAGGATTT+TGG + chr1.2:75678485-75678504 None:intergenic 35.0%
! TGGTAGATTACGACCATTTT+GGG - chr1.2:75676319-75676338 MS.gene34460:CDS 35.0%
!! GCTTTGTGTATATTTGCTGT+AGG - chr1.2:75678443-75678462 MS.gene34460:CDS 35.0%
!! TGTATATTTGCTGTAGGGTA+TGG - chr1.2:75678449-75678468 MS.gene34460:CDS 35.0%
!! TTTTGGGGATGTTGTTTGTT+TGG - chr1.2:75676335-75676354 MS.gene34460:CDS 35.0%
!!! TGGTTTTGAAACCAATGAAG+TGG - chr1.2:75678061-75678080 MS.gene34460:CDS 35.0%
AAATTTACCTGCTCCAGAGA+GGG - chr1.2:75675802-75675821 MS.gene34460:intron 40.0%
AACATTGAACCCCATCAATC+TGG + chr1.2:75675731-75675750 None:intergenic 40.0%
AATGGCTCTCAAACTCAATC+TGG - chr1.2:75675607-75675626 MS.gene34460:intron 40.0%
AATGTCTGGGAAAAAGCCTA+AGG - chr1.2:75676509-75676528 MS.gene34460:CDS 40.0%
ACAAGTATGCCAGATTGATG+GGG - chr1.2:75675719-75675738 MS.gene34460:CDS 40.0%
AGAATTTACTCATGCTTGGG+GGG - chr1.2:75676719-75676738 MS.gene34460:CDS 40.0%
AGAGATGGACAAAAGATGCA+AGG - chr1.2:75677327-75677346 MS.gene34460:CDS 40.0%
AGCGAAATGAATGGTTGAGA+TGG - chr1.2:75676766-75676785 MS.gene34460:CDS 40.0%
AGGGAAAGAGATATGGGTAA+AGG - chr1.2:75676171-75676190 MS.gene34460:intron 40.0%
AGTAAATTCTTCCTCGTCCT+TGG + chr1.2:75676709-75676728 None:intergenic 40.0%
AGTCAGAACTTCGGTATCTA+TGG + chr1.2:75678524-75678543 None:intergenic 40.0%
ATCTCAACCATTCATTTCGC+TGG + chr1.2:75676767-75676786 None:intergenic 40.0%
ATGAAACATATGGAGATGGG+GGG + chr1.2:75677796-75677815 None:intergenic 40.0%
ATGCAAGGTTGGGTAATTTC+AGG - chr1.2:75677342-75677361 MS.gene34460:CDS 40.0%
ATGGACAAAAGATGCAAGGT+TGG - chr1.2:75677331-75677350 MS.gene34460:CDS 40.0%
ATGGTCACACAGCAAAATAC+AGG + chr1.2:75678505-75678524 None:intergenic 40.0%
CAACTTGACGAAGTTATGGA+AGG - chr1.2:75677500-75677519 MS.gene34460:CDS 40.0%
CATCTGATTATTGCTTGGCA+CGG + chr1.2:75678322-75678341 None:intergenic 40.0%
CATGCATTGAACCACTTCAT+TGG + chr1.2:75678075-75678094 None:intergenic 40.0%
CCACAGACTTGTCAAGTTAT+CGG - chr1.2:75677433-75677452 MS.gene34460:CDS 40.0%
CCTCCACTATCCTTTCAAAA+TGG + chr1.2:75676938-75676957 None:intergenic 40.0%
CGATAACCCACCAAAAGTTT+GGG + chr1.2:75678393-75678412 None:intergenic 40.0%
GAGTAAGCGAAGAAATTGAC+AGG - chr1.2:75677663-75677682 MS.gene34460:CDS 40.0%
GAGTTGTATATATGACCCCA+AGG - chr1.2:75676689-75676708 MS.gene34460:CDS 40.0%
GATGAAACATATGGAGATGG+GGG + chr1.2:75677797-75677816 None:intergenic 40.0%
GGATGTCTTCCAAAGCTAAT+GGG - chr1.2:75676999-75677018 MS.gene34460:CDS 40.0%
GTAAATTCTTCCTCGTCCTT+GGG + chr1.2:75676708-75676727 None:intergenic 40.0%
TAAATTCTTCCTCGTCCTTG+GGG + chr1.2:75676707-75676726 None:intergenic 40.0%
TACATGGTATGGTGGTTTCT+AGG - chr1.2:75675779-75675798 MS.gene34460:intron 40.0%
TACTGTGAAGAAACATCGGA+AGG - chr1.2:75675852-75675871 MS.gene34460:CDS 40.0%
TAGAATAGAAGTTTGGCGGT+TGG + chr1.2:75678148-75678167 None:intergenic 40.0%
TAGGAGTAAGGTACATGGTA+TGG - chr1.2:75675768-75675787 MS.gene34460:intron 40.0%
TCTGCCAAACACATGTATAC+CGG + chr1.2:75676599-75676618 None:intergenic 40.0%
TCTGTGAGGGAAAGAGATAT+GGG - chr1.2:75676165-75676184 MS.gene34460:intron 40.0%
TGCAAGGTTGGGTAATTTCA+GGG - chr1.2:75677343-75677362 MS.gene34460:CDS 40.0%
TGGACAAAAGATGCAAGGTT+GGG - chr1.2:75677332-75677351 MS.gene34460:CDS 40.0%
TTCTGTGAGGGAAAGAGATA+TGG - chr1.2:75676164-75676183 MS.gene34460:intron 40.0%
TTTCTGGGCTGATGACAATA+TGG - chr1.2:75676296-75676315 MS.gene34460:CDS 40.0%
! ACCACATTACCCATTAGCTT+TGG + chr1.2:75677011-75677030 None:intergenic 40.0%
! CGGCCATTTGTACAGTTTTT+AGG - chr1.2:75676387-75676406 MS.gene34460:CDS 40.0%
! GGTAGATTACGACCATTTTG+GGG - chr1.2:75676320-75676339 MS.gene34460:CDS 40.0%
! GTGGTAGATTACGACCATTT+TGG - chr1.2:75676318-75676337 MS.gene34460:CDS 40.0%
! TCCAAAGCTAATGGGTAATG+TGG - chr1.2:75677007-75677026 MS.gene34460:CDS 40.0%
! TCTGGTAATGCATCAGCAAA+TGG - chr1.2:75675625-75675644 MS.gene34460:intron 40.0%
! TGGCTGTTTCGAACTTTCAT+TGG - chr1.2:75676483-75676502 MS.gene34460:CDS 40.0%
!! AGCTTGCTACAACCATCTTA+GGG + chr1.2:75677397-75677416 None:intergenic 40.0%
!! ATTTTGTGTTGCTCAAGCTG+TGG - chr1.2:75676020-75676039 MS.gene34460:intron 40.0%
!! CCATTTTGAAAGGATAGTGG+AGG - chr1.2:75676935-75676954 MS.gene34460:CDS 40.0%
!! TGGTTTTACCTGAAACAGAC+CGG - chr1.2:75676577-75676596 MS.gene34460:CDS 40.0%
!! TGTTCTGTGATAATGGCCTT+AGG + chr1.2:75676528-75676547 None:intergenic 40.0%
!!! CAAACTTTTGGTGGGTTATC+GGG - chr1.2:75678392-75678411 MS.gene34460:CDS 40.0%
!!! CCAAACTTTTGGTGGGTTAT+CGG - chr1.2:75678391-75678410 MS.gene34460:CDS 40.0%
AAAGGCAAGGAGAACTCTGT+TGG + chr1.2:75678172-75678191 None:intergenic 45.0%
AACCCACCAAAAGTTTGGGA+AGG + chr1.2:75678389-75678408 None:intergenic 45.0%
AAGAGCTTCTGAATCTGTGG+AGG - chr1.2:75677457-75677476 MS.gene34460:CDS 45.0%
ACACCTGCGATAGTTGAGAA+AGG + chr1.2:75678190-75678209 None:intergenic 45.0%
ACCCACCAAAAGTTTGGGAA+GGG + chr1.2:75678388-75678407 None:intergenic 45.0%
ACTTTCATTGGCGCAATGTC+TGG - chr1.2:75676495-75676514 MS.gene34460:CDS 45.0%
AGAGCTTCTGAATCTGTGGA+GGG - chr1.2:75677458-75677477 MS.gene34460:CDS 45.0%
CAGACCGGTATACATGTGTT+TGG - chr1.2:75676592-75676611 MS.gene34460:CDS 45.0%
CATGTATACCGGTCTGTTTC+AGG + chr1.2:75676588-75676607 None:intergenic 45.0%
CCATTAGCGTGAAGAACGTT+CGG + chr1.2:75675592-75675611 None:intergenic 45.0%
CCGATAACCCACCAAAAGTT+TGG + chr1.2:75678394-75678413 None:intergenic 45.0%
CCGATAACTTGACAAGTCTG+TGG + chr1.2:75677436-75677455 None:intergenic 45.0%
CTTACCTGCAAAGGGAGATT+TGG + chr1.2:75677824-75677843 None:intergenic 45.0%
CTTTCATTGGCGCAATGTCT+GGG - chr1.2:75676496-75676515 MS.gene34460:CDS 45.0%
GAAATTTACCTGCTCCAGAG+AGG - chr1.2:75675801-75675820 MS.gene34460:intron 45.0%
GAGGCAACTTGACGAAGTTA+TGG - chr1.2:75677496-75677515 MS.gene34460:CDS 45.0%
GAGTAAGGTACATGGTATGG+TGG - chr1.2:75675771-75675790 MS.gene34460:intron 45.0%
GATATGGGTAAAGGAGAAGC+TGG - chr1.2:75676180-75676199 MS.gene34460:intron 45.0%
GGGATGTCTTCCAAAGCTAA+TGG - chr1.2:75676998-75677017 MS.gene34460:CDS 45.0%
GGTAATGCATCAGCAAATGG+AGG - chr1.2:75675628-75675647 MS.gene34460:intron 45.0%
GTCAACACAGTGTTCGGAAT+CGG - chr1.2:75675655-75675674 MS.gene34460:intron 45.0%
GTCAACCAGCACAAAAGAGA+TGG - chr1.2:75677116-75677135 MS.gene34460:CDS 45.0%
GTCATCAACATCGAGCTGTA+TGG + chr1.2:75676262-75676281 None:intergenic 45.0%
GTGCTTCAAAATGGGTAACC+TGG + chr1.2:75675936-75675955 None:intergenic 45.0%
TATATATGACCCCAAGGACG+AGG - chr1.2:75676695-75676714 MS.gene34460:CDS 45.0%
TCAACCAAATCTCCCTTTGC+AGG - chr1.2:75677817-75677836 MS.gene34460:CDS 45.0%
TGCGATAGTTGAGAAAGGCA+AGG + chr1.2:75678185-75678204 None:intergenic 45.0%
TTGCCTTTCTCAACTATCGC+AGG - chr1.2:75678184-75678203 MS.gene34460:CDS 45.0%
TTTCCTACGATATCCCTCTC+TGG + chr1.2:75675818-75675837 None:intergenic 45.0%
! AAACCCCCTTCCCAAACTTT+TGG - chr1.2:75678380-75678399 MS.gene34460:CDS 45.0%
! CGGACTCATGGTATGTGTTT+TGG + chr1.2:75678302-75678321 None:intergenic 45.0%
! CGTTCGGACTGCAACAATTT+TGG + chr1.2:75675576-75675595 None:intergenic 45.0%
! GCAGAACAGACAGAGCTTTT+CGG - chr1.2:75676367-75676386 MS.gene34460:CDS 45.0%
! GGCTTTGTCAACACAGTGTT+CGG - chr1.2:75675649-75675668 MS.gene34460:intron 45.0%
!! AGGGTTATCAGTTTGCTGTG+AGG - chr1.2:75677477-75677496 MS.gene34460:CDS 45.0%
!! CAGCTTGCTACAACCATCTT+AGG + chr1.2:75677398-75677417 None:intergenic 45.0%
AATTGACAGGACCGCTACAC+TGG - chr1.2:75677676-75677695 MS.gene34460:CDS 50.0%
ACTGGATAGGTGCCAACCAA+CGG - chr1.2:75677694-75677713 MS.gene34460:CDS 50.0%
AGAAGTTTGGCGGTTGGAAC+AGG + chr1.2:75678142-75678161 None:intergenic 50.0%
AGGAGAAGCTGGACGTTTGA+TGG - chr1.2:75676191-75676210 MS.gene34460:intron 50.0%
CACAGTGTTCGGAATCGGTA+CGG - chr1.2:75675660-75675679 MS.gene34460:intron 50.0%
CCACCAAAAGTTTGGGAAGG+GGG + chr1.2:75678386-75678405 None:intergenic 50.0%
CCCACCAAAAGTTTGGGAAG+GGG + chr1.2:75678387-75678406 None:intergenic 50.0%
CCGAACGTTCTTCACGCTAA+TGG - chr1.2:75675589-75675608 MS.gene34460:intron 50.0%
CTGGGCTGATGACAATATGG+TGG - chr1.2:75676299-75676318 MS.gene34460:CDS 50.0%
GGAGAAGCTGGACGTTTGAT+GGG - chr1.2:75676192-75676211 MS.gene34460:intron 50.0%
GGAGACGAGAACTGGATGTT+TGG - chr1.2:75675873-75675892 MS.gene34460:CDS 50.0%
GGCAAGAGCTTCTGAATCTG+TGG - chr1.2:75677454-75677473 MS.gene34460:CDS 50.0%
GGGTTATCGGGTGTTTATGG+TGG - chr1.2:75678404-75678423 MS.gene34460:CDS 50.0%
TATTGCTTGGCACGGACTCA+TGG + chr1.2:75678314-75678333 None:intergenic 50.0%
TCCTCAGTCGCATGTCCATT+CGG + chr1.2:75677626-75677645 None:intergenic 50.0%
TGCTTCAGAAAGTGAGCCTG+CGG - chr1.2:75677592-75677611 MS.gene34460:CDS 50.0%
TTGGCACCTATCCAGTGTAG+CGG + chr1.2:75677690-75677709 None:intergenic 50.0%
! CCCCTTCCCAAACTTTTGGT+GGG - chr1.2:75678384-75678403 MS.gene34460:CDS 50.0%
! TTGTGTTGCTCAAGCTGTGG+AGG - chr1.2:75676023-75676042 MS.gene34460:intron 50.0%
!! GAGAAATGGGCTGTCTGCTT+TGG - chr1.2:75676804-75676823 MS.gene34460:CDS 50.0%
!! GGTGGGTTATCGGGTGTTTA+TGG - chr1.2:75678401-75678420 MS.gene34460:CDS 50.0%
ACAGGACCGCTACACTGGAT+AGG - chr1.2:75677681-75677700 MS.gene34460:CDS 55.0%
CATCGGAAGGAGACGAGAAC+TGG - chr1.2:75675865-75675884 MS.gene34460:CDS 55.0%
CCATTCGGAAAGATCACCGC+AGG + chr1.2:75677611-75677630 None:intergenic 55.0%
CCTGCGGTGATCTTTCCGAA+TGG - chr1.2:75677608-75677627 MS.gene34460:CDS 55.0%
GCTCCAGAGAGGGATATCGT+AGG - chr1.2:75675812-75675831 MS.gene34460:intron 55.0%
TCATGCTTGGGGGGTTATGC+TGG - chr1.2:75676728-75676747 MS.gene34460:CDS 55.0%
TCCGAATGGACATGCGACTG+AGG - chr1.2:75677622-75677641 MS.gene34460:CDS 55.0%
TGGACATGCGACTGAGGATC+AGG - chr1.2:75677628-75677647 MS.gene34460:CDS 55.0%
! CCCCCTTCCCAAACTTTTGG+TGG - chr1.2:75678383-75678402 MS.gene34460:CDS 55.0%
!! ACGATATCCCTCTCTGGAGC+AGG + chr1.2:75675812-75675831 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 75675481 75678551 75675481 ID=MS.gene34460
chr1.2 mRNA 75675481 75678551 75675481 ID=MS.gene34460.t1;Parent=MS.gene34460
chr1.2 exon 75676194 75678551 75676194 ID=MS.gene34460.t1.exon1;Parent=MS.gene34460.t1
chr1.2 CDS 75676194 75678551 75676194 ID=cds.MS.gene34460.t1;Parent=MS.gene34460.t1
chr1.2 exon 75675827 75675979 75675827 ID=MS.gene34460.t1.exon2;Parent=MS.gene34460.t1
chr1.2 CDS 75675827 75675979 75675827 ID=cds.MS.gene34460.t1;Parent=MS.gene34460.t1
chr1.2 exon 75675685 75675744 75675685 ID=MS.gene34460.t1.exon3;Parent=MS.gene34460.t1
chr1.2 CDS 75675685 75675744 75675685 ID=cds.MS.gene34460.t1;Parent=MS.gene34460.t1
chr1.2 exon 75675481 75675567 75675481 ID=MS.gene34460.t1.exon4;Parent=MS.gene34460.t1
chr1.2 CDS 75675481 75675567 75675481 ID=cds.MS.gene34460.t1;Parent=MS.gene34460.t1
Gene Sequence

>MS.gene34460

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATGTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTCGGAATCGGTACGGTATTTGAGTCTGATGAACATGCATATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGTGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACGAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGATGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGGTTGACTCATATCATAATTTGGGACCCAAATCTAGTTCAGCTTTAAGTATGACGAATACAAAATTAGGTGCACGTGATTCTCTTGATCAACTTTCCATGAATTATGACAATTATCTTCATTCTGTGAGGGAAAGAGATATGGGTAAAGGAGAAGCTGGACGTTTGATGGGTTATTTTCAAAGGCAACACTTTGAAAATCCAGCATTTTTTTATGCCATACAGCTCGATGTTGATGACAAGATAACAAATCTTTTCTGGGCTGATGACAATATGGTGGTAGATTACGACCATTTTGGGGATGTTGTTTGTTTGGATACAACTTGCAGAACAGACAGAGCTTTTCGGCCATTTGTACAGTTTTTAGGTGTTAATCATCACAAACAAGTATTAATATTTGCTGCTGCTTTTTTGTATGATGAGACCATCGAATCATTTAATTGGCTGTTTCGAACTTTCATTGGCGCAATGTCTGGGAAAAAGCCTAAGGCCATTATCACAGAACAAGATGCTGCAATCATCGAAGCAATCAATGTGGTTTTACCTGAAACAGACCGGTATACATGTGTTTGGCAGATGTATGAGAACACTCTTAAACATCTCAGCCATTTGGTTAAAGATGACGAATCCTTTGCAAATGATCTAAGGAGTTGTATATATGACCCCAAGGACGAGGAAGAATTTACTCATGCTTGGGGGGTTATGCTGGAGAAATATAAACTCCAGCGAAATGAATGGTTGAGATGGATGTATAGAGAATGGGAGAAATGGGCTGTCTGCTTTGGTCAGAATAGATTTTTTGTAGATGTCAAAGGCTTTCATTTAGGTGAGATGTTGTCTCACAAGCTTAGAAGCTATCTAAATCCTGACCTTGATGTAGTTCAATTTCTTAACCATTTTGAAAGGATAGTGGAGGAGCAACGATATAAAGAAATAGAAGCTAGTGATGAAATGAAGGGATGTCTTCCAAAGCTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGTATAAAGTGAATACATTTGGACATGCTAGACAGTACACTGTCACCTTCAATTCTTCAGATAATACAGTTGTCTGCAGTTGTATGAAGTTTGAGCATGTAGGTTTTCTTTGTAGCCATGCACTAAAAGTTCTTGATAATAGAAACATAAAAGTAGTTCCATCTCGATATATCTTGAAGAGATGGACAAAAGATGCAAGGTTGGGTAATTTCAGGGAGATTAAGCAATTTAAAATTCAAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGGTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACAGGACCGCTACACTGGATAGGTGCCAACCAACGGTGAATTATAATCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCATGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCCTTTGCAGGTAAGCTTTTATCATTTATTTGATTGATATATGGTAGCTTGTATTCTTGTGATCATATAGAGCATATTTGATATAGAGATCAGGAAGTTTAATGCTCTTTCTTTTTTTGCAATGAAGTTTTATGCTCGTTAACTTTTGGAGAATATGCTCTGTATAATTTTGAAATAAAAAATAAAGAGAACGGCATTTGTTGATTTTCTCATTTGTTTTACAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTAGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCTTGCCTTTCTCAACTATCGCAGGTGTTAATTTTTCCAGTCATTAGGTTTTTTTTATATGCAGAATATGTTACTTTGTATAACATATCATGAATATTTTACTCAGGAGCCTATAATCCAAAACACATACCATGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGTAAACATTTACTTAATGGTCATAATTTGAACAAACCCCCTTCCCAAACTTTTGGTGGGTTATCGGGTGTTTATGGTGGAAAACTAATTGCATCTGCTTTGTGTATATTTGCTGTAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATTTTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA
Protein sequence

>MS.gene34460.t1

MDGDHNNLGNVHVENVDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVFGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMVVSRKFTCSREGYRRKDERDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVEVDSYHNLGPKSSSALSMTNTKLGARDSLDQLSMNYDNYLHSVRERDMGKGEAGRLMGYFQRQHFENPAFFYAIQLDVDDKITNLFWADDNMVVDYDHFGDVVCLDTTCRTDRAFRPFVQFLGVNHHKQVLIFAAAFLYDETIESFNWLFRTFIGAMSGKKPKAIITEQDAAIIEAINVVLPETDRYTCVWQMYENTLKHLSHLVKDDESFANDLRSCIYDPKDEEEFTHAWGVMLEKYKLQRNEWLRWMYREWEKWAVCFGQNRFFVDVKGFHLGEMLSHKLRSYLNPDLDVVQFLNHFERIVEEQRYKEIEASDEMKGCLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEYKVNTFGHARQYTVTFNSSDNTVVCSCMKFEHVGFLCSHALKVLDNRNIKVVPSRYILKRWTKDARLGNFREIKQFKIQDNPKMVVASCYKDLCHRLVKLSARASESVEGYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATLDRCQPTVNYNQTDSDRILNVEPSSNTVVCISCPPSPYVSSQPNLPLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYHESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA