AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34481

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34481.t1 MTR_1g100653 88.889 216 14 1 12 227 89 294 9.43e-139 391
MS.gene34481.t1 MTR_7g076740 42.291 227 92 8 12 216 90 299 4.17e-37 132
MS.gene34481.t1 MTR_8g031360 37.555 229 93 9 12 213 91 296 9.41e-35 126
MS.gene34481.t1 MTR_7g011170 49.485 97 37 3 12 97 89 184 4.52e-19 84.7
MS.gene34481.t1 MTR_6g012690 54.217 83 30 3 12 86 89 171 1.85e-16 77.8
MS.gene34481.t1 MTR_4g082290 51.190 84 33 1 12 87 89 172 2.42e-16 77.8
MS.gene34481.t1 MTR_4g082230 51.190 84 33 1 12 87 89 172 2.56e-16 77.8
MS.gene34481.t1 MTR_1g076150 69.444 36 11 0 7 42 32 67 6.89e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34481.t1 AT3G61250 42.326 215 85 8 12 213 89 277 1.54e-33 123
MS.gene34481.t1 AT3G01140 53.012 83 33 1 12 88 89 171 7.86e-19 84.3
MS.gene34481.t1 AT3G01140 53.012 83 33 1 12 88 132 214 9.33e-19 84.7
MS.gene34481.t1 AT5G15310 51.807 83 34 1 12 88 10 92 1.99e-18 82.0
MS.gene34481.t1 AT5G15310 51.807 83 34 1 12 88 89 171 1.57e-17 80.5
MS.gene34481.t1 AT5G15310 51.807 83 34 1 12 88 89 171 1.64e-17 80.5
MS.gene34481.t1 AT5G15310 51.807 83 34 1 12 88 89 171 2.83e-17 78.6
MS.gene34481.t1 AT5G55020 65.854 41 14 0 12 52 103 143 4.82e-11 62.4

Find 61 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGACACGTTTCTTTAAA+TGG 0.094066 1.2:+75449927 None:intergenic
GCTTTGGTGTCGACACTTTC+AGG 0.182498 1.2:+75449570 None:intergenic
AAGCTTCAGTTTGTAGTTAC+TGG 0.214754 1.2:+75449514 None:intergenic
ATCACTATCATCATCGAAAT+TGG 0.245454 1.2:-75449642 MS.gene34481:CDS
GAGTCCACTTTGCTTAATTC+AGG 0.272638 1.2:-75449779 MS.gene34481:CDS
TGAAGATGTTGACGTGATTC+AGG 0.278646 1.2:-75449546 MS.gene34481:CDS
GATAGATCTGGTTCAGATTC+AGG 0.282629 1.2:-75449485 MS.gene34481:CDS
CTGCGTATGCCTGATGAAAT+AGG 0.293386 1.2:-75449416 MS.gene34481:CDS
ATCTCATTGTCTGTTCTTCC+TGG 0.294103 1.2:+75449968 None:intergenic
CTGATTACTTTCTTCAACTT+TGG 0.298456 1.2:-75449736 MS.gene34481:CDS
CAACCCTTGCACTTTCCCAC+TGG 0.302795 1.2:+75449825 None:intergenic
GCCTGATGAAATAGGATTCC+TGG 0.344813 1.2:-75449408 MS.gene34481:CDS
ACATGCTGATACGGGTCTTC+GGG 0.364216 1.2:+75450873 None:intergenic
GACATGCTGATACGGGTCTT+CGG 0.368775 1.2:+75450872 None:intergenic
AATTTCTTGAATCTCCTTGA+TGG 0.383750 1.2:-75449374 MS.gene34481:intron
CCGGACACGTTTCTTTAAAT+GGG 0.388694 1.2:+75449928 None:intergenic
ACAATGAGATTAAGAACTAT+TGG 0.389201 1.2:-75449955 MS.gene34481:CDS
TTAGACTGGGCATTCCTATC+AGG 0.389920 1.2:+75449872 None:intergenic
AAGACCCGTATCAGCATGTC+TGG 0.394376 1.2:-75450869 MS.gene34481:CDS
GTCCATGTTTAGAGGGGAAA+AGG 0.407013 1.2:-75449086 MS.gene34481:CDS
AACCCTTGCACTTTCCCACT+GGG 0.410798 1.2:+75449826 None:intergenic
CAACTTTGGCATTCTGATGT+TGG 0.417033 1.2:-75449722 MS.gene34481:CDS
TGGGCCATGTGGCGTGTAGA+AGG 0.420239 1.2:+75449845 None:intergenic
CAAAGTTGAAGAAAGTAATC+AGG 0.443367 1.2:+75449737 None:intergenic
ATGTTCAGATGTCTCATTGC+AGG 0.446382 1.2:-75449615 MS.gene34481:CDS
ATCCATGTCTCCTTCCTGAT+AGG 0.449112 1.2:-75449886 MS.gene34481:CDS
CCCAGGAATCCTATTTCATC+AGG 0.453232 1.2:+75449407 None:intergenic
AACGTGTCCGGCAGTCACTA+AGG 0.458737 1.2:-75449916 MS.gene34481:CDS
ACGTCAACATCTTCAAGCTT+TGG 0.459604 1.2:+75449554 None:intergenic
CAAACCCAGACATGCTGATA+CGG 0.459905 1.2:+75450864 None:intergenic
GTGTAGAAGGAGAATTAGAC+TGG 0.475010 1.2:+75449858 None:intergenic
GAAAAGGGTGGACAGAGAAA+TGG 0.476368 1.2:-75449070 MS.gene34481:CDS
AAGCTTGAAGATGATAGATC+TGG 0.524702 1.2:-75449497 MS.gene34481:CDS
CCTGATGAAATAGGATTCCT+GGG 0.529222 1.2:-75449407 MS.gene34481:CDS
AGCAGAGACGAGGCTGTCGT+TGG 0.533698 1.2:-75449801 MS.gene34481:CDS
CCCATTTAAAGAAACGTGTC+CGG 0.543989 1.2:-75449928 MS.gene34481:CDS
CACTTTCCCACTGGGCCATG+TGG 0.548011 1.2:+75449834 None:intergenic
GCTATAGCAGCTCAACTACC+AGG 0.548333 1.2:-75449986 MS.gene34481:CDS
TCCATGTTTAGAGGGGAAAA+GGG 0.552840 1.2:-75449085 MS.gene34481:CDS
TGGCCCAGTGGGAAAGTGCA+AGG 0.553498 1.2:-75449829 MS.gene34481:CDS
AAGGTGAGGTCCATGTTTAG+AGG 0.558450 1.2:-75449094 MS.gene34481:CDS
AGGTGAGGTCCATGTTTAGA+GGG 0.560803 1.2:-75449093 MS.gene34481:CDS
CTTAGTCACATCTACCATCA+AGG 0.561878 1.2:+75449360 None:intergenic
AGAAATTGTCAGTATGTCCC+AGG 0.567136 1.2:+75449390 None:intergenic
AAACCCAGACATGCTGATAC+GGG 0.568045 1.2:+75450865 None:intergenic
TTCCTATCAGGAAGGAGACA+TGG 0.573818 1.2:+75449884 None:intergenic
GTTGGAGAGAAATTTCGAGC+AGG 0.578155 1.2:-75449704 MS.gene34481:CDS
ACGTGTCCGGCAGTCACTAA+GGG 0.585904 1.2:-75449915 MS.gene34481:CDS
TTCTCCTTCTACACGCCACA+TGG 0.594285 1.2:-75449849 MS.gene34481:CDS
TACACGCCACATGGCCCAGT+GGG 0.601016 1.2:-75449840 MS.gene34481:CDS
GGCCCAGTGGGAAAGTGCAA+GGG 0.612508 1.2:-75449828 MS.gene34481:CDS
CAAGGGTTGAAGCAGAGACG+AGG 0.615393 1.2:-75449811 MS.gene34481:CDS
AGAGCCTGAATTAAGCAAAG+TGG 0.615810 1.2:+75449775 None:intergenic
TTTCATCAGGCATACGCAGT+AGG 0.616891 1.2:+75449420 None:intergenic
ATGTTTAGAGGGGAAAAGGG+TGG 0.662181 1.2:-75449082 MS.gene34481:CDS
ACTGGGCATTCCTATCAGGA+AGG 0.663389 1.2:+75449876 None:intergenic
AGACCCGTATCAGCATGTCT+GGG 0.665685 1.2:-75450868 MS.gene34481:intron
TGACTTCCAAAGGTAAGGTG+AGG 0.668297 1.2:-75449108 MS.gene34481:intron
GGTGAGGTCCATGTTTAGAG+GGG 0.695996 1.2:-75449092 MS.gene34481:CDS
TGTAGAAGGAGAATTAGACT+GGG 0.703666 1.2:+75449859 None:intergenic
CTACACGCCACATGGCCCAG+TGG 0.712167 1.2:-75449841 MS.gene34481:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGAAGATATCACAAAA+AGG + chr1.2:75450792-75450811 None:intergenic 20.0%
!!! CAAAACAAAACTTTTACAAT+AGG + chr1.2:75449255-75449274 None:intergenic 20.0%
! ACAATGAGATTAAGAACTAT+TGG - chr1.2:75449989-75450008 MS.gene34481:CDS 25.0%
! TAATAATAACCTATTGAGAG+AGG + chr1.2:75449181-75449200 None:intergenic 25.0%
! TATGTATGTTTACTACAAGT+AGG - chr1.2:75449650-75449669 MS.gene34481:CDS 25.0%
! TTATATTGATCTGACACTTA+TGG - chr1.2:75449526-75449545 MS.gene34481:CDS 25.0%
!! AGTTATACTTTTCCTTATCT+CGG + chr1.2:75449463-75449482 None:intergenic 25.0%
AATCTTGTCTTAAATAGACC+CGG - chr1.2:75449379-75449398 MS.gene34481:CDS 30.0%
AATTGACCTAGCCAAAATTT+AGG - chr1.2:75449133-75449152 MS.gene34481:intron 30.0%
AATTTCTTGAATCTCCTTGA+TGG - chr1.2:75450570-75450589 MS.gene34481:intron 30.0%
ATCACTATCATCATCGAAAT+TGG - chr1.2:75450302-75450321 MS.gene34481:intron 30.0%
ATGTTAACTCTTTGCATACA+TGG + chr1.2:75449579-75449598 None:intergenic 30.0%
ATTGACCTAGCCAAAATTTA+GGG - chr1.2:75449134-75449153 MS.gene34481:intron 30.0%
CAAAATTTAGGGACATGTTA+GGG - chr1.2:75449145-75449164 MS.gene34481:intron 30.0%
CAAAGTTGAAGAAAGTAATC+AGG + chr1.2:75450210-75450229 None:intergenic 30.0%
CAGTGTCAAGAAAAATTTGT+TGG - chr1.2:75449880-75449899 MS.gene34481:CDS 30.0%
CTGATTACTTTCTTCAACTT+TGG - chr1.2:75450208-75450227 MS.gene34481:intron 30.0%
GATAACTATGCTATCTTTCT+AGG - chr1.2:75449929-75449948 MS.gene34481:CDS 30.0%
GGACAATTGCAACAAATTTA+AGG + chr1.2:75449340-75449359 None:intergenic 30.0%
TAACTAACATAGTGCATGTT+TGG + chr1.2:75450709-75450728 None:intergenic 30.0%
TGTGAAATGTATAACAGTTG+AGG - chr1.2:75449217-75449236 MS.gene34481:intron 30.0%
TGTTTGGTATCACAAAATCA+CGG - chr1.2:75450611-75450630 MS.gene34481:intron 30.0%
! TAGCTTTTGCAAAATCATGA+TGG + chr1.2:75450641-75450660 None:intergenic 30.0%
!! TTGAGATTGAGTTGGTTTAA+AGG + chr1.2:75449361-75449380 None:intergenic 30.0%
AACTATGCTATCTTTCTAGG+TGG - chr1.2:75449932-75449951 MS.gene34481:CDS 35.0%
AAGCTTCAGTTTGTAGTTAC+TGG + chr1.2:75450433-75450452 None:intergenic 35.0%
AAGCTTGAAGATGATAGATC+TGG - chr1.2:75450447-75450466 MS.gene34481:intron 35.0%
ACTATGCTATCTTTCTAGGT+GGG - chr1.2:75449933-75449952 MS.gene34481:CDS 35.0%
AGACAAGATTGAGATTGAGT+TGG + chr1.2:75449369-75449388 None:intergenic 35.0%
AGTTTGACCTAGTAATCTCA+AGG + chr1.2:75449505-75449524 None:intergenic 35.0%
ATAGTGCATGTTTGGTATCA+CGG + chr1.2:75450701-75450720 None:intergenic 35.0%
CCAAAATTTAGGGACATGTT+AGG - chr1.2:75449144-75449163 MS.gene34481:intron 35.0%
GAAATGTATAACAGTTGAGG+TGG - chr1.2:75449220-75449239 MS.gene34481:intron 35.0%
GATGTGACTAAGTACATGTT+TGG - chr1.2:75450595-75450614 MS.gene34481:intron 35.0%
GCAACACATTTAGTTACTGT+AGG + chr1.2:75449557-75449576 None:intergenic 35.0%
TATGAGTGGAATGAACCTTT+AGG + chr1.2:75449839-75449858 None:intergenic 35.0%
TCAACTCATCTTAGTCCTAA+AGG - chr1.2:75449821-75449840 MS.gene34481:CDS 35.0%
TCTAAAGAATGACCGAGATA+AGG - chr1.2:75449448-75449467 MS.gene34481:CDS 35.0%
TGTAGAAGGAGAATTAGACT+GGG + chr1.2:75450088-75450107 None:intergenic 35.0%
TTAGTCTCTTCAAGAGAGAA+AGG - chr1.2:75450726-75450745 MS.gene34481:intron 35.0%
TTTACTAAGCAGGTTATGAG+TGG + chr1.2:75449853-75449872 None:intergenic 35.0%
! CCTAACATGTCCCTAAATTT+TGG + chr1.2:75449147-75449166 None:intergenic 35.0%
! GAAGTATTACCTCTCTCAAT+AGG - chr1.2:75449169-75449188 MS.gene34481:intron 35.0%
!!! TTGTTTTGAGTTTGAATGCG+TGG - chr1.2:75449267-75449286 MS.gene34481:intron 35.0%
ACGTCAACATCTTCAAGCTT+TGG + chr1.2:75450393-75450412 None:intergenic 40.0%
AGAAATTGTCAGTATGTCCC+AGG + chr1.2:75450557-75450576 None:intergenic 40.0%
AGAGCCTGAATTAAGCAAAG+TGG + chr1.2:75450172-75450191 None:intergenic 40.0%
ATCTCATTGTCTGTTCTTCC+TGG + chr1.2:75449979-75449998 None:intergenic 40.0%
ATGTTCAGATGTCTCATTGC+AGG - chr1.2:75450329-75450348 MS.gene34481:intron 40.0%
ATTCAACTTCATGCCATGCT+TGG - chr1.2:75449773-75449792 MS.gene34481:CDS 40.0%
CAACTTTGGCATTCTGATGT+TGG - chr1.2:75450222-75450241 MS.gene34481:intron 40.0%
CCCATTTAAAGAAACGTGTC+CGG - chr1.2:75450016-75450035 MS.gene34481:intron 40.0%
CCGGACACGTTTCTTTAAAT+GGG + chr1.2:75450019-75450038 None:intergenic 40.0%
CCTGATGAAATAGGATTCCT+GGG - chr1.2:75450537-75450556 MS.gene34481:intron 40.0%
CTGCAATGCATTTACTAAGC+AGG + chr1.2:75449863-75449882 None:intergenic 40.0%
CTTAGTCACATCTACCATCA+AGG + chr1.2:75450587-75450606 None:intergenic 40.0%
GACAGTGTATCAGAGAAAGA+TGG - chr1.2:75449601-75449620 MS.gene34481:CDS 40.0%
GAGTCCACTTTGCTTAATTC+AGG - chr1.2:75450165-75450184 MS.gene34481:intron 40.0%
GTGTAGAAGGAGAATTAGAC+TGG + chr1.2:75450089-75450108 None:intergenic 40.0%
TCCATGTTTAGAGGGGAAAA+GGG - chr1.2:75450859-75450878 MS.gene34481:intron 40.0%
TGAAGATGTTGACGTGATTC+AGG - chr1.2:75450398-75450417 MS.gene34481:intron 40.0%
TGACCTAGTAATCTCAAGGT+AGG + chr1.2:75449501-75449520 None:intergenic 40.0%
TTTGGAAGTCAGCTATATGG+TGG + chr1.2:75450827-75450846 None:intergenic 40.0%
! ACCATATAGCTGACTTCCAA+AGG - chr1.2:75450826-75450845 MS.gene34481:intron 40.0%
! ACCTTTGGAAGTCAGCTATA+TGG + chr1.2:75450830-75450849 None:intergenic 40.0%
! ATAGCTGACTTCCAAAGGTA+AGG - chr1.2:75450831-75450850 MS.gene34481:intron 40.0%
! GATAGATCTGGTTCAGATTC+AGG - chr1.2:75450459-75450478 MS.gene34481:intron 40.0%
!! GAGTTGATAATAGAGCAGTG+AGG + chr1.2:75449808-75449827 None:intergenic 40.0%
!! GCTACAAAGTGTTGCTTTGT+AGG - chr1.2:75450657-75450676 MS.gene34481:intron 40.0%
!!! CATGTCCCTAAATTTTGGCT+AGG + chr1.2:75449142-75449161 None:intergenic 40.0%
AAACCCAGACATGCTGATAC+GGG + chr1.2:75449082-75449101 None:intergenic 45.0%
AAGGTGAGGTCCATGTTTAG+AGG - chr1.2:75450850-75450869 MS.gene34481:intron 45.0%
AGGTGAGGTCCATGTTTAGA+GGG - chr1.2:75450851-75450870 MS.gene34481:intron 45.0%
ATCCATGTCTCCTTCCTGAT+AGG - chr1.2:75450058-75450077 MS.gene34481:intron 45.0%
ATGTTTAGAGGGGAAAAGGG+TGG - chr1.2:75450862-75450881 MS.gene34481:intron 45.0%
CAAACCCAGACATGCTGATA+CGG + chr1.2:75449083-75449102 None:intergenic 45.0%
CAAGTAGGTCTCCTAAGATG+CGG - chr1.2:75449665-75449684 MS.gene34481:CDS 45.0%
CCCAGGAATCCTATTTCATC+AGG + chr1.2:75450540-75450559 None:intergenic 45.0%
CTGCGTATGCCTGATGAAAT+AGG - chr1.2:75450528-75450547 MS.gene34481:intron 45.0%
GAAAAGGGTGGACAGAGAAA+TGG - chr1.2:75450874-75450893 MS.gene34481:CDS 45.0%
GATAGTTTATCCACCGAAGC+CGG + chr1.2:75449700-75449719 None:intergenic 45.0%
GCCGGACACGTTTCTTTAAA+TGG + chr1.2:75450020-75450039 None:intergenic 45.0%
GCCTGATGAAATAGGATTCC+TGG - chr1.2:75450536-75450555 MS.gene34481:intron 45.0%
GCTCCTACCTTGAGATTACT+AGG - chr1.2:75449495-75449514 MS.gene34481:CDS 45.0%
GTCCATGTTTAGAGGGGAAA+AGG - chr1.2:75450858-75450877 MS.gene34481:intron 45.0%
GTTGGAGAGAAATTTCGAGC+AGG - chr1.2:75450240-75450259 MS.gene34481:intron 45.0%
TAAGATGCGGCAAAAGTTGC+CGG - chr1.2:75449678-75449697 MS.gene34481:CDS 45.0%
TCGGGTCTAGCAATGAGTTA+GGG - chr1.2:75449104-75449123 MS.gene34481:intron 45.0%
TGACTTCCAAAGGTAAGGTG+AGG - chr1.2:75450836-75450855 MS.gene34481:intron 45.0%
TGGATAAACTATCTCCGTCC+TGG - chr1.2:75449707-75449726 MS.gene34481:CDS 45.0%
TTAGACTGGGCATTCCTATC+AGG + chr1.2:75450075-75450094 None:intergenic 45.0%
TTCCTATCAGGAAGGAGACA+TGG + chr1.2:75450063-75450082 None:intergenic 45.0%
TTCGGGTCTAGCAATGAGTT+AGG - chr1.2:75449103-75449122 MS.gene34481:intron 45.0%
TTTCATCAGGCATACGCAGT+AGG + chr1.2:75450527-75450546 None:intergenic 45.0%
! ACCCTTTTCCCCTCTAAACA+TGG + chr1.2:75450863-75450882 None:intergenic 45.0%
! ATCGGGTTTGTTCGTGTCTA+GGG - chr1.2:75449411-75449430 MS.gene34481:CDS 45.0%
! GTTTTTCCCTTAGTGACTGC+CGG + chr1.2:75450038-75450057 None:intergenic 45.0%
! TCAGCATGTCTGGGTTTGTT+CGG - chr1.2:75449085-75449104 MS.gene34481:CDS 45.0%
AACCCTTGCACTTTCCCACT+GGG + chr1.2:75450121-75450140 None:intergenic 50.0%
AAGACCCGTATCAGCATGTC+TGG - chr1.2:75449075-75449094 MS.gene34481:CDS 50.0%
ACATGCTGATACGGGTCTTC+GGG + chr1.2:75449074-75449093 None:intergenic 50.0%
ACTGGGCATTCCTATCAGGA+AGG + chr1.2:75450071-75450090 None:intergenic 50.0%
AGACCCGTATCAGCATGTCT+GGG - chr1.2:75449076-75449095 MS.gene34481:CDS 50.0%
CATGGACCTCACCTTACCTT+TGG + chr1.2:75450845-75450864 None:intergenic 50.0%
CTCAAGGTAGGAGCGAACAA+GGG + chr1.2:75449489-75449508 None:intergenic 50.0%
GACATGCTGATACGGGTCTT+CGG + chr1.2:75449075-75449094 None:intergenic 50.0%
GCTATAGCAGCTCAACTACC+AGG - chr1.2:75449958-75449977 MS.gene34481:CDS 50.0%
GGTGAGGTCCATGTTTAGAG+GGG - chr1.2:75450852-75450871 MS.gene34481:intron 50.0%
TCTCAAGGTAGGAGCGAACA+AGG + chr1.2:75449490-75449509 None:intergenic 50.0%
TGTCTAGGGACGACGAGTTT+GGG - chr1.2:75449425-75449444 MS.gene34481:CDS 50.0%
TTCATGCCATGCTTGGCAAC+AGG - chr1.2:75449780-75449799 MS.gene34481:CDS 50.0%
TTCTCCTTCTACACGCCACA+TGG - chr1.2:75450095-75450114 MS.gene34481:intron 50.0%
! CAGCATGTCTGGGTTTGTTC+GGG - chr1.2:75449086-75449105 MS.gene34481:CDS 50.0%
! CATCGGGTTTGTTCGTGTCT+AGG - chr1.2:75449410-75449429 MS.gene34481:CDS 50.0%
! GGCAACTTTTGCCGCATCTT+AGG + chr1.2:75449679-75449698 None:intergenic 50.0%
!! GCTTTGGTGTCGACACTTTC+AGG + chr1.2:75450377-75450396 None:intergenic 50.0%
AAACCCGATGCTCTACGACC+GGG + chr1.2:75449400-75449419 None:intergenic 55.0%
AACGTGTCCGGCAGTCACTA+AGG - chr1.2:75450028-75450047 MS.gene34481:intron 55.0%
AATGAGCCACGCTTGATGCC+AGG + chr1.2:75449728-75449747 None:intergenic 55.0%
ACGTGTCCGGCAGTCACTAA+GGG - chr1.2:75450029-75450048 MS.gene34481:intron 55.0%
CAAACCCGATGCTCTACGAC+CGG + chr1.2:75449401-75449420 None:intergenic 55.0%
CAACCCTTGCACTTTCCCAC+TGG + chr1.2:75450122-75450141 None:intergenic 55.0%
CAAGGGTTGAAGCAGAGACG+AGG - chr1.2:75450133-75450152 MS.gene34481:intron 55.0%
GTGTCTAGGGACGACGAGTT+TGG - chr1.2:75449424-75449443 MS.gene34481:CDS 55.0%
TAGACCCGGTCGTAGAGCAT+CGG - chr1.2:75449393-75449412 MS.gene34481:CDS 55.0%
! GAGGTACCTGTTGCCAAGCA+TGG + chr1.2:75449789-75449808 None:intergenic 55.0%
AGCAGAGACGAGGCTGTCGT+TGG - chr1.2:75450143-75450162 MS.gene34481:intron 60.0%
AGCCACGCTTGATGCCAGGA+CGG + chr1.2:75449724-75449743 None:intergenic 60.0%
CACTTTCCCACTGGGCCATG+TGG + chr1.2:75450113-75450132 None:intergenic 60.0%
TACACGCCACATGGCCCAGT+GGG - chr1.2:75450104-75450123 MS.gene34481:intron 60.0%
TGGGCCATGTGGCGTGTAGA+AGG + chr1.2:75450102-75450121 None:intergenic 60.0%
! AGACCCGGTCGTAGAGCATC+GGG - chr1.2:75449394-75449413 MS.gene34481:CDS 60.0%
! GGCCCAGTGGGAAAGTGCAA+GGG - chr1.2:75450116-75450135 MS.gene34481:intron 60.0%
! TGGCCCAGTGGGAAAGTGCA+AGG - chr1.2:75450115-75450134 MS.gene34481:intron 60.0%
!! GCAAAAGTTGCCGGCTTCGG+TGG - chr1.2:75449687-75449706 MS.gene34481:CDS 60.0%
!! GCGGCAAAAGTTGCCGGCTT+CGG - chr1.2:75449684-75449703 MS.gene34481:CDS 60.0%
CTACACGCCACATGGCCCAG+TGG - chr1.2:75450103-75450122 MS.gene34481:intron 65.0%
CTCCGTCCTGGCATCAAGCG+TGG - chr1.2:75449719-75449738 MS.gene34481:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 75449066 75450900 75449066 ID=MS.gene34481
chr1.2 mRNA 75449066 75450900 75449066 ID=MS.gene34481.t1;Parent=MS.gene34481
chr1.2 exon 75450869 75450900 75450869 ID=MS.gene34481.t1.exon1;Parent=MS.gene34481.t1
chr1.2 CDS 75450869 75450900 75450869 ID=cds.MS.gene34481.t1;Parent=MS.gene34481.t1
chr1.2 exon 75449375 75450015 75449375 ID=MS.gene34481.t1.exon2;Parent=MS.gene34481.t1
chr1.2 CDS 75449375 75450015 75449375 ID=cds.MS.gene34481.t1;Parent=MS.gene34481.t1
chr1.2 exon 75449066 75449118 75449066 ID=MS.gene34481.t1.exon3;Parent=MS.gene34481.t1
chr1.2 CDS 75449066 75449118 75449066 ID=cds.MS.gene34481.t1;Parent=MS.gene34481.t1
Gene Sequence

>MS.gene34481

ATGAACCCGAAGACCCGTATCAGCATGTCTGGGTTTGTTCGGGTCTAGCAATGAGTTAGGGTCTTAGAATTGACCTAGCCAAAATTTAGGGACATGTTAGGGTGAAGTATTACCTCTCTCAATAGGTTATTATTATAAACAAAAAAATCATTGTGAAATGTATAACAGTTGAGGTGGAAATTAAGTCCTATTGTAAAAGTTTTGTTTTGAGTTTGAATGCGTGGTAGAATATAAAAAACAAATCTAACTTTTAGTTATAATTGTGTGATTACCTTAAATTTGTTGCAATTGTCCTTTAAACCAACTCAATCTCAATCTTGTCTTAAATAGACCCGGTCGTAGAGCATCGGGTTTGTTCGTGTCTAGGGACGACGAGTTTGGGTCTAAAGAATGACCGAGATAAGGAAAAGTATAACTCGTCCCTTGTTCGCTCCTACCTTGAGATTACTAGGTCAAACTTTTATATTGATCTGACACTTATGGCATTGCCTACAGTAACTAAATGTGTTGCCATGTATGCAAAGAGTTAACATAAGACAGTGTATCAGAGAAAGATGGATTCTTACAACTTATTAATATAATTTTATGTATGTTTACTACAAGTAGGTCTCCTAAGATGCGGCAAAAGTTGCCGGCTTCGGTGGATAAACTATCTCCGTCCTGGCATCAAGCGTGGCTCATTTACTAATGAAGAAGAAACTACTATTATTCAACTTCATGCCATGCTTGGCAACAGGTACCTCACTGCTCTATTATCAACTCATCTTAGTCCTAAAGGTTCATTCCACTCATAACCTGCTTAGTAAATGCATTGCAGTGTCAAGAAAAATTTGTTGGATCTAACATTCACTTTGCATGTAACTGATAACTATGCTATCTTTCTAGGTGGGCTGCTATAGCAGCTCAACTACCAGGAAGAACAGACAATGAGATTAAGAACTATTGGAACACCCATTTAAAGAAACGTGTCCGGCAGTCACTAAGGGAAAAACATCCATGTCTCCTTCCTGATAGGAATGCCCAGTCTAATTCTCCTTCTACACGCCACATGGCCCAGTGGGAAAGTGCAAGGGTTGAAGCAGAGACGAGGCTGTCGTTGGAGTCCACTTTGCTTAATTCAGGCTCTACGACTAAAACATATCCTGATTACTTTCTTCAACTTTGGCATTCTGATGTTGGAGAGAAATTTCGAGCAGGAGTAGTGTGTCAAAGCATTGTCTCACAAGCATCATCATCATCACTATCATCATCGAAATTGGAGTCATGTTCAGATGTCTCATTGCAGGTGAAAAACAACGAAACACAAAGCCTGAAAGTGTCGACACCAAAGCTTGAAGATGTTGACGTGATTCAGGAACAAAAAGCCAGTAACTACAAACTGAAGCTTGAAGATGATAGATCTGGTTCAGATTCAGGAAATTATGAGTTTCTTGATACTTCTGATTCTGCACTGAAGCACCTACTGCGTATGCCTGATGAAATAGGATTCCTGGGACATACTGACAATTTCTTGAATCTCCTTGATGGTAGATGTGACTAAGTACATGTTTGGTATCACAAAATCACGGTGAGCCATCATGATTTTGCAAAAGCTACAAAGTGTTGCTTTGTAGGCTTTTTAGCATTTGCAAACCGTGATACCAAACATGCACTATGTTAGTTAGTCTCTTCAAGAGAGAAAGGCAAATACTCTGATATATGATAGAATAGTTAGTTTTCTCAGCCTTTTTGTGATATCTTCATTTTTGAAAAATGCATCCACCATATAGCTGACTTCCAAAGGTAAGGTGAGGTCCATGTTTAGAGGGGAAAAGGGTGGACAGAGAAATGGCTAG
Protein sequence

>MS.gene34481.t1

MNPKTRISMSGWAAIAAQLPGRTDNEIKNYWNTHLKKRVRQSLREKHPCLLPDRNAQSNSPSTRHMAQWESARVEAETRLSLESTLLNSGSTTKTYPDYFLQLWHSDVGEKFRAGVVCQSIVSQASSSSLSSSKLESCSDVSLQVKNNETQSLKVSTPKLEDVDVIQEQKASNYKLKLEDDRSGSDSGNYEFLDTSDSALKHLLRMPDEIGFLGHTDNFLNLLDGKVRSMFRGEKGGQRNG