Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34758.t1 | XP_013445809.1 | 81 | 279 | 50 | 1 | 1 | 279 | 1 | 276 | 4.60E-100 | 374.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34758.t1 | Q9LFQ3 | 40.8 | 103 | 58 | 2 | 111 | 211 | 328 | 429 | 3.1e-12 | 73.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34758.t1 | A0A072U246 | 81.0 | 279 | 50 | 1 | 1 | 279 | 1 | 276 | 3.3e-100 | 374.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene34758.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34758.t1 | MTR_8g469180 | 83.871 | 279 | 42 | 1 | 1 | 279 | 1 | 276 | 2.40e-169 | 470 |
| MS.gene34758.t1 | MTR_8g062700 | 55.172 | 87 | 39 | 0 | 193 | 279 | 332 | 418 | 3.80e-23 | 99.4 |
| MS.gene34758.t1 | MTR_8g062700 | 34.392 | 189 | 92 | 3 | 36 | 192 | 227 | 415 | 5.34e-23 | 99.0 |
| MS.gene34758.t1 | MTR_8g062700 | 64.384 | 73 | 26 | 0 | 193 | 265 | 332 | 404 | 3.74e-22 | 96.7 |
| MS.gene34758.t1 | MTR_8g062700 | 33.862 | 189 | 92 | 4 | 36 | 192 | 227 | 414 | 8.03e-21 | 92.8 |
| MS.gene34758.t1 | MTR_5g036670 | 50.000 | 86 | 41 | 1 | 195 | 278 | 320 | 405 | 5.19e-19 | 87.4 |
| MS.gene34758.t1 | MTR_5g036670 | 50.000 | 78 | 36 | 2 | 118 | 192 | 327 | 404 | 8.63e-14 | 72.0 |
| MS.gene34758.t1 | MTR_6g008970 | 46.237 | 93 | 50 | 0 | 187 | 279 | 332 | 424 | 6.67e-19 | 87.0 |
| MS.gene34758.t1 | MTR_6g008970 | 47.561 | 82 | 43 | 0 | 111 | 192 | 340 | 421 | 2.35e-17 | 82.4 |
| MS.gene34758.t1 | MTR_6g008995 | 38.255 | 149 | 54 | 3 | 79 | 189 | 197 | 345 | 2.50e-18 | 84.7 |
| MS.gene34758.t1 | MTR_6g008995 | 51.351 | 74 | 36 | 0 | 198 | 271 | 270 | 343 | 2.19e-17 | 82.0 |
| MS.gene34758.t1 | MTR_6g008995 | 38.255 | 149 | 54 | 3 | 79 | 189 | 140 | 288 | 2.80e-18 | 84.3 |
| MS.gene34758.t1 | MTR_6g008995 | 51.351 | 74 | 36 | 0 | 198 | 271 | 213 | 286 | 2.71e-17 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34758.t1 | AT5G15020 | 40.566 | 106 | 56 | 1 | 164 | 269 | 304 | 402 | 2.07e-16 | 79.7 |
| MS.gene34758.t1 | AT5G15020 | 42.045 | 88 | 49 | 1 | 111 | 196 | 328 | 415 | 3.46e-14 | 73.2 |
| MS.gene34758.t1 | AT5G15020 | 40.566 | 106 | 56 | 1 | 164 | 269 | 304 | 402 | 2.25e-16 | 79.7 |
| MS.gene34758.t1 | AT5G15020 | 42.045 | 88 | 49 | 1 | 111 | 196 | 328 | 415 | 3.53e-14 | 73.2 |
| MS.gene34758.t1 | AT3G01320 | 44.706 | 85 | 47 | 0 | 180 | 264 | 317 | 401 | 1.01e-15 | 77.8 |
| MS.gene34758.t1 | AT3G01320 | 40.385 | 104 | 58 | 3 | 111 | 211 | 332 | 434 | 5.26e-14 | 72.4 |
| MS.gene34758.t1 | AT3G01320 | 52.174 | 69 | 33 | 0 | 196 | 264 | 321 | 389 | 1.36e-15 | 77.4 |
| MS.gene34758.t1 | AT3G01320 | 38.393 | 112 | 65 | 3 | 103 | 211 | 312 | 422 | 4.74e-14 | 72.8 |
| MS.gene34758.t1 | AT1G24190 | 46.341 | 82 | 43 | 1 | 198 | 279 | 287 | 367 | 3.05e-15 | 76.3 |
| MS.gene34758.t1 | AT1G24190 | 41.026 | 78 | 45 | 1 | 115 | 192 | 288 | 364 | 1.00e-12 | 68.6 |
| MS.gene34758.t1 | AT1G24190 | 46.341 | 82 | 43 | 1 | 198 | 279 | 287 | 367 | 3.10e-15 | 76.3 |
| MS.gene34758.t1 | AT1G24190 | 41.026 | 78 | 45 | 1 | 115 | 192 | 288 | 364 | 9.91e-13 | 68.6 |
| MS.gene34758.t1 | AT1G24190 | 50.685 | 73 | 35 | 1 | 198 | 270 | 287 | 358 | 3.77e-15 | 75.9 |
| MS.gene34758.t1 | AT1G24190 | 41.772 | 79 | 45 | 1 | 115 | 193 | 288 | 365 | 3.30e-13 | 70.1 |
| MS.gene34758.t1 | AT1G70060 | 47.945 | 73 | 37 | 1 | 198 | 270 | 303 | 374 | 2.25e-14 | 73.6 |
| MS.gene34758.t1 | AT1G70060 | 47.945 | 73 | 37 | 1 | 198 | 270 | 303 | 374 | 2.25e-14 | 73.6 |
Find 55 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGGTGGATGATTTAT+TGG | 0.144756 | 8.3:-41187743 | MS.gene34758:intron |
| CCGAGAGGAATTACAATTAT+TGG | 0.178616 | 8.3:-41188186 | MS.gene34758:intron |
| ATCAAACCTTTGTTAATTTC+TGG | 0.213353 | 8.3:+41194852 | None:intergenic |
| ATCAAACCTTTGTTAAATTC+TGG | 0.224271 | 8.3:+41194960 | None:intergenic |
| GTCAGCAGCTTCATTATCTT+TGG | 0.238299 | 8.3:+41196650 | None:intergenic |
| GGGAAAATATACCGATCTTA+TGG | 0.254179 | 8.3:-41187722 | MS.gene34758:CDS |
| CTTCAAAGCTTTAACAAGTA+TGG | 0.279212 | 8.3:+41187657 | None:intergenic |
| AAACAATCCTGAAAATTATC+AGG | 0.286860 | 8.3:-41188252 | MS.gene34758:CDS |
| AGTAGACAGCGTGGGGCGTT+TGG | 0.314226 | 8.3:+41196614 | None:intergenic |
| CAACAGGTGGATGATTTATT+GGG | 0.325941 | 8.3:-41187742 | MS.gene34758:intron |
| GTAGACAGCGTGGGGCGTTT+GGG | 0.348314 | 8.3:+41196615 | None:intergenic |
| CATACTTGTTAAAGCTTTGA+AGG | 0.355544 | 8.3:-41187656 | MS.gene34758:CDS |
| TTGCAAATCGTTGGCCTTCT+CGG | 0.356912 | 8.3:+41195016 | None:intergenic |
| CCTTTGTTAATTTCTGGATC+AGG | 0.378179 | 8.3:+41194858 | None:intergenic |
| GGAAAATATACCGATCTTAT+GGG | 0.409782 | 8.3:-41187721 | MS.gene34758:CDS |
| ACCTCTCCGTGACTTTGTTA+AGG | 0.425146 | 8.3:+41194663 | None:intergenic |
| CTTTCAGATAGGCGATTTGT+TGG | 0.441089 | 8.3:-41189278 | MS.gene34758:intron |
| TTTATTAGGAATTTGTTTGT+CGG | 0.457283 | 8.3:-41195047 | MS.gene34758:intron |
| TTCCCATTCTGTAGCAATGT+AGG | 0.471794 | 8.3:+41194603 | None:intergenic |
| GTCCCTACATTGCTACAGAA+TGG | 0.480728 | 8.3:-41194606 | MS.gene34758:CDS |
| TTCTTGAAACAGAAATGAAA+AGG | 0.481600 | 8.3:-41196674 | None:intergenic |
| GCGATCACCTTTCGTATCAA+TGG | 0.487977 | 8.3:+41194899 | None:intergenic |
| TCCCTACATTGCTACAGAAT+GGG | 0.488103 | 8.3:-41194605 | MS.gene34758:CDS |
| TTTCAGATAGGCGATTTGTT+GGG | 0.503365 | 8.3:-41189277 | MS.gene34758:intron |
| CATGACACAAAGTGAAAAGA+AGG | 0.508610 | 8.3:-41187683 | MS.gene34758:CDS |
| CCAATAATTGTAATTCCTCT+CGG | 0.508805 | 8.3:+41188186 | None:intergenic |
| AACAAAGTCACGGAGAGGTT+AGG | 0.509807 | 8.3:-41194659 | MS.gene34758:CDS |
| CAATAATTGTAATTCCTCTC+GGG | 0.513671 | 8.3:+41188187 | None:intergenic |
| AAGGTAAGAAAATGCTTCAC+TGG | 0.513839 | 8.3:+41194682 | None:intergenic |
| TCCCATTCTGTAGCAATGTA+GGG | 0.526266 | 8.3:+41194604 | None:intergenic |
| AGGTTTGATGAGTGCAAATG+AGG | 0.530693 | 8.3:-41194838 | MS.gene34758:CDS |
| TTTGGTGTCTCAGGTATGTA+CGG | 0.531859 | 8.3:-41188323 | MS.gene34758:intron |
| ATGATCACCTTGCAAATCGT+TGG | 0.554360 | 8.3:+41195007 | None:intergenic |
| ATACTTGTTAAAGCTTTGAA+GGG | 0.554714 | 8.3:-41187655 | MS.gene34758:CDS |
| ATTAAATTCGCCCATAAGAT+CGG | 0.555210 | 8.3:+41187711 | None:intergenic |
| TTCTTACCTTAACAAAGTCA+CGG | 0.564749 | 8.3:-41194669 | MS.gene34758:CDS |
| TTGACACAATGTGAATCCAA+TGG | 0.569616 | 8.3:-41189217 | MS.gene34758:intron |
| GTGTCTCAGGTATGTACGGT+TGG | 0.570587 | 8.3:-41188319 | MS.gene34758:intron |
| CTTGATCCAGAATTTAACAA+AGG | 0.573154 | 8.3:-41194966 | MS.gene34758:CDS |
| GGGAAAACATTCTGATCTTG+TGG | 0.578356 | 8.3:-41189257 | MS.gene34758:CDS |
| TCTTGTGTAGTAGACAGCGT+GGG | 0.582235 | 8.3:+41196606 | None:intergenic |
| AAACATTCTGATCTTGTGGA+GGG | 0.596716 | 8.3:-41189253 | MS.gene34758:CDS |
| CAAAGGTTTGATAAGTGCAG+AGG | 0.606415 | 8.3:-41194949 | MS.gene34758:CDS |
| CCTGATCCAGAAATTAACAA+AGG | 0.616646 | 8.3:-41194858 | MS.gene34758:CDS |
| AAGACAAGAACTACAAACAA+TGG | 0.616882 | 8.3:-41194573 | MS.gene34758:intron |
| AAAACATTCTGATCTTGTGG+AGG | 0.627867 | 8.3:-41189254 | MS.gene34758:CDS |
| GTCTTGTGTAGTAGACAGCG+TGG | 0.639850 | 8.3:+41196605 | None:intergenic |
| GATCAGGATGTTCGACAATG+AGG | 0.651290 | 8.3:+41194874 | None:intergenic |
| TGTCGGACTGAGTACCGAGA+AGG | 0.658623 | 8.3:-41195030 | MS.gene34758:CDS |
| GAGAAGGCCAACGATTTGCA+AGG | 0.661652 | 8.3:-41195014 | MS.gene34758:CDS |
| ACCTTAACAAAGTCACGGAG+AGG | 0.667295 | 8.3:-41194664 | MS.gene34758:CDS |
| CTTGTGTAGTAGACAGCGTG+GGG | 0.673923 | 8.3:+41196607 | None:intergenic |
| CAGCAAAATAATTACCCGAG+AGG | 0.690212 | 8.3:-41188201 | MS.gene34758:CDS |
| GACATGACCATTGATACGAA+AGG | 0.732031 | 8.3:-41194906 | MS.gene34758:CDS |
| GGAGAGGTTAGGAATACACG+AGG | 0.778238 | 8.3:-41194648 | MS.gene34758:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 41187642 | 41196682 | 41187642 | ID=MS.gene34758 |
| chr8.3 | mRNA | 41187642 | 41196682 | 41187642 | ID=MS.gene34758.t1;Parent=MS.gene34758 |
| chr8.3 | exon | 41196603 | 41196682 | 41196603 | ID=MS.gene34758.t1.exon1;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41196603 | 41196682 | 41196603 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41194808 | 41195061 | 41194808 | ID=MS.gene34758.t1.exon2;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41194808 | 41195061 | 41194808 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41194574 | 41194710 | 41194574 | ID=MS.gene34758.t1.exon3;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41194574 | 41194710 | 41194574 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41189218 | 41189293 | 41189218 | ID=MS.gene34758.t1.exon4;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41189218 | 41189293 | 41189218 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41189102 | 41189131 | 41189102 | ID=MS.gene34758.t1.exon5;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41189102 | 41189131 | 41189102 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41188187 | 41188332 | 41188187 | ID=MS.gene34758.t1.exon6;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41188187 | 41188332 | 41188187 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| chr8.3 | exon | 41187642 | 41187758 | 41187642 | ID=MS.gene34758.t1.exon7;Parent=MS.gene34758.t1 |
| chr8.3 | CDS | 41187642 | 41187758 | 41187642 | ID=cds.MS.gene34758.t1;Parent=MS.gene34758.t1 |
| Gene Sequence |
| Protein sequence |
