Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34823.t1 | XP_003628592.1 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 1.30E-245 | 858.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34823.t1 | Q9AR19 | 66.3 | 403 | 130 | 4 | 46 | 446 | 170 | 568 | 6.6e-158 | 558.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34823.t1 | G7LEM3 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 9.3e-246 | 858.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene34823.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34823.t1 | MTR_8g062330 | 92.617 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 0.0 | 860 |
| MS.gene34823.t1 | MTR_7g096040 | 75.556 | 405 | 93 | 5 | 43 | 446 | 143 | 542 | 0.0 | 644 |
| MS.gene34823.t1 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 344 | 434 | 85 | 176 | 6.70e-13 | 70.9 |
| MS.gene34823.t1 | MTR_8g005100 | 42.857 | 84 | 46 | 2 | 358 | 441 | 98 | 179 | 2.90e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34823.t1 | AT3G54610 | 66.917 | 399 | 121 | 5 | 50 | 446 | 179 | 568 | 0.0 | 574 |
| MS.gene34823.t1 | AT2G34900 | 34.906 | 106 | 65 | 3 | 331 | 434 | 104 | 207 | 7.34e-12 | 67.4 |
| MS.gene34823.t1 | AT2G34900 | 34.694 | 98 | 61 | 2 | 344 | 439 | 6 | 102 | 8.98e-12 | 65.9 |
| MS.gene34823.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene34823.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene34823.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 4.46e-11 | 65.9 |
| MS.gene34823.t1 | AT3G01770 | 27.317 | 205 | 115 | 7 | 251 | 438 | 43 | 230 | 5.07e-11 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTCTATAATGGTTATA+AGG | + | 57430:28266-28285 | MS.gene34823:CDS | 20.0% |
| !! | GTTATATAAAGGATTATGAT+GGG | + | 57430:28548-28567 | MS.gene34823:CDS | 20.0% |
| !! | TAAGTCTATAATGGTTATAA+GGG | + | 57430:28267-28286 | MS.gene34823:CDS | 20.0% |
| !! | TGAAATCATAAAAAATCCAA+TGG | + | 57430:28966-28985 | MS.gene34823:CDS | 20.0% |
| !! | TTATAACCATTATAGACTTA+TGG | - | 57430:28267-28286 | None:intergenic | 20.0% |
| !! | TTATATAAAGGATTATGATG+GGG | + | 57430:28549-28568 | MS.gene34823:CDS | 20.0% |
| !! | TTTATGATTTCATAATAACC+AGG | - | 57430:28958-28977 | None:intergenic | 20.0% |
| !!! | ATAGTTGATAAATCAGTATA+AGG | - | 57430:28610-28629 | None:intergenic | 20.0% |
| !!! | CATTTGTAATATATGGTTTT+TGG | - | 57430:29102-29121 | None:intergenic | 20.0% |
| !!! | TAAACTCAGTTAAATGTTTT+AGG | - | 57430:28858-28877 | None:intergenic | 20.0% |
| ! | ATGTCATCAACAATTCTATT+AGG | - | 57430:28745-28764 | None:intergenic | 25.0% |
| ! | GGTTATATAAAGGATTATGA+TGG | + | 57430:28547-28566 | MS.gene34823:CDS | 25.0% |
| ! | TATATAAAGGATTATGATGG+GGG | + | 57430:28550-28569 | MS.gene34823:CDS | 25.0% |
| ! | TATGCTGATAATAATGCTAT+TGG | + | 57430:28466-28485 | MS.gene34823:CDS | 25.0% |
| ! | TGTAGCACATTTGTAATATA+TGG | - | 57430:29109-29128 | None:intergenic | 25.0% |
| !! | TTCAGTGAATATATTTGTTG+CGG | - | 57430:28041-28060 | None:intergenic | 25.0% |
| !!! | AATGTTTTAGGTTTGTAACA+TGG | - | 57430:28846-28865 | None:intergenic | 25.0% |
| !!! | TTGTAATATATGGTTTTTGG+AGG | - | 57430:29099-29118 | None:intergenic | 25.0% |
| AAATCAGTATAAGGAAGCTT+TGG | - | 57430:28601-28620 | None:intergenic | 30.0% | |
| AGTGATGATCAACAAGTAAA+AGG | + | 57430:28370-28389 | MS.gene34823:CDS | 30.0% | |
| ATTTACAAGAGAGATTCACT+TGG | + | 57430:28507-28526 | MS.gene34823:CDS | 30.0% | |
| ATTTATCAACTATGATCCGT+CGG | + | 57430:28617-28636 | MS.gene34823:CDS | 30.0% | |
| GATCAACAAGTAAAAGGTTA+TGG | + | 57430:28376-28395 | MS.gene34823:CDS | 30.0% | |
| GTTTATGCGTTCAATTCTAA+AGG | + | 57430:28873-28892 | MS.gene34823:CDS | 30.0% | |
| TAGGAACCATAAGTCTATAA+TGG | + | 57430:28258-28277 | MS.gene34823:CDS | 30.0% | |
| TGTCACAATATTGTTTATCC+TGG | + | 57430:28682-28701 | MS.gene34823:CDS | 30.0% | |
| ! | ATTGGCTATTTCATCAAACA+AGG | + | 57430:28484-28503 | MS.gene34823:CDS | 30.0% |
| !! | TATGGTTTTTGGAGGATTAT+AGG | - | 57430:29091-29110 | None:intergenic | 30.0% |
| ACTGAAAAATTGCAGACAAG+AGG | + | 57430:28055-28074 | MS.gene34823:CDS | 35.0% | |
| ATCGTCCATATGCAAATCAA+AGG | + | 57430:28320-28339 | MS.gene34823:CDS | 35.0% | |
| ATCTAAAGACAATGAGTGAG+AGG | + | 57430:28989-29008 | MS.gene34823:CDS | 35.0% | |
| ATGCTATCAACGAAACCATA+TGG | - | 57430:28171-28190 | None:intergenic | 35.0% | |
| CTCATTGTCTTTAGATCCAT+TGG | - | 57430:28985-29004 | None:intergenic | 35.0% | |
| CTTAAGAAAGAGGAAGAATC+TGG | + | 57430:28103-28122 | MS.gene34823:CDS | 35.0% | |
| TCGATGGGAAGGTTATATAA+AGG | + | 57430:28537-28556 | MS.gene34823:CDS | 35.0% | |
| TGCATTCTCAAACATTCTTC+TGG | - | 57430:29064-29083 | None:intergenic | 35.0% | |
| !! | TGTCACTTTGCTTTGAAAGT+GGG | - | 57430:29142-29161 | None:intergenic | 35.0% |
| !! | TTGTCACTTTGCTTTGAAAG+TGG | - | 57430:29143-29162 | None:intergenic | 35.0% |
| CTCAAACATTCTTCTGGCAT+CGG | - | 57430:29058-29077 | None:intergenic | 40.0% | |
| CTCTTCTGAACATCAATTCC+AGG | - | 57430:28703-28722 | None:intergenic | 40.0% | |
| GAAAAATTGCAGACAAGAGG+TGG | + | 57430:28058-28077 | MS.gene34823:CDS | 40.0% | |
| GGAAGAGACTCTTAAGAAAG+AGG | + | 57430:28093-28112 | MS.gene34823:CDS | 40.0% | |
| GGTTCCTATCCATAACAAGT+CGG | - | 57430:28246-28265 | None:intergenic | 40.0% | |
| TTCCTCCTTAACCACAAAGT+CGG | - | 57430:28017-28036 | None:intergenic | 40.0% | |
| TTTCTTAAGAGTCTCTTCCC+TGG | - | 57430:28092-28111 | None:intergenic | 40.0% | |
| TTTGAATGCGTGTGCAATGA+TGG | + | 57430:28136-28155 | MS.gene34823:CDS | 40.0% | |
| !! | AGAATTGTTGATGACATCCC+TGG | + | 57430:28748-28767 | MS.gene34823:CDS | 40.0% |
| !! | GCGAACCTTTGATTTGCATA+TGG | - | 57430:28328-28347 | None:intergenic | 40.0% |
| !! | GTACATTGTCCGACTTGTTA+TGG | + | 57430:28234-28253 | MS.gene34823:CDS | 40.0% |
| !! | TGATGGTGTTGATGACCATA+TGG | + | 57430:28153-28172 | MS.gene34823:CDS | 40.0% |
| !! | TTGTCCGACTTGTTATGGAT+AGG | + | 57430:28239-28258 | MS.gene34823:CDS | 40.0% |
| !!! | ACTGAATTTTGAAGCCAGAC+TGG | - | 57430:29173-29192 | None:intergenic | 40.0% |
| AAAGACAATGAGTGAGAGGC+TGG | + | 57430:28993-29012 | MS.gene34823:CDS | 45.0% | |
| AAGAAGTGGTGAAGGCAGAA+GGG | - | 57430:27871-27890 | None:intergenic | 45.0% | |
| AAGTCGGACAATGTACTCCT+TGG | - | 57430:28230-28249 | None:intergenic | 45.0% | |
| AATGTACTCCTTGGGCATGT+TGG | - | 57430:28221-28240 | None:intergenic | 45.0% | |
| AGTCGGACAATGTACTCCTT+GGG | - | 57430:28229-28248 | None:intergenic | 45.0% | |
| ATCCAGCCTCTTTCAAACCA+GGG | - | 57430:28768-28787 | None:intergenic | 45.0% | |
| ATGTACTCCTTGGGCATGTT+GGG | - | 57430:28220-28239 | None:intergenic | 45.0% | |
| CAAGCGCAAACATGTATCTG+AGG | + | 57430:27898-27917 | MS.gene34823:CDS | 45.0% | |
| CAGATACATGTTTGCGCTTG+TGG | - | 57430:27898-27917 | None:intergenic | 45.0% | |
| CAGTATGCACGTGATGTAGA+TGG | + | 57430:28424-28443 | MS.gene34823:CDS | 45.0% | |
| CTGATATGTACGGTCATTCG+CGG | + | 57430:28794-28813 | MS.gene34823:CDS | 45.0% | |
| GCATCAACAGGTTCCTTGAA+TGG | - | 57430:28925-28944 | None:intergenic | 45.0% | |
| TACATGTTTGCGCTTGTGGA+TGG | - | 57430:27894-27913 | None:intergenic | 45.0% | |
| TCACTTGGAGAGAGATCGAT+GGG | + | 57430:28522-28541 | MS.gene34823:CDS | 45.0% | |
| TCCTCCTTAACCACAAAGTC+GGG | - | 57430:28016-28035 | None:intergenic | 45.0% | |
| TCTTCGATGCACAACTTCAC+CGG | + | 57430:27953-27972 | MS.gene34823:CDS | 45.0% | |
| TTCACTTGGAGAGAGATCGA+TGG | + | 57430:28521-28540 | MS.gene34823:CDS | 45.0% | |
| ! | GGATGGACTCCTGATATGTA+CGG | + | 57430:28784-28803 | MS.gene34823:CDS | 45.0% |
| ! | TCATTCGCGGTTTAGAGCTT+TGG | + | 57430:28807-28826 | MS.gene34823:CDS | 45.0% |
| ! | TGACATCCCTGGTTTGAAAG+AGG | + | 57430:28759-28778 | MS.gene34823:CDS | 45.0% |
| ! | TTATGATGGGGGAACTCTCA+TGG | + | 57430:28561-28580 | MS.gene34823:CDS | 45.0% |
| !!! | GAATTTTGAAGCCAGACTGG+AGG | - | 57430:29170-29189 | None:intergenic | 45.0% |
| AATGAGTGAGAGGCTGGAGT+CGG | + | 57430:28999-29018 | MS.gene34823:CDS | 50.0% | |
| ACAACTTCCCAACATGCCCA+AGG | + | 57430:28210-28229 | MS.gene34823:CDS | 50.0% | |
| ATAGTGCCTGTCTTTGCCGA+CGG | - | 57430:28636-28655 | None:intergenic | 50.0% | |
| CAGAAGAGATCAGAAGCTGC+AGG | + | 57430:28715-28734 | MS.gene34823:CDS | 50.0% | |
| CATCCAGCCTCTTTCAAACC+AGG | - | 57430:28769-28788 | None:intergenic | 50.0% | |
| CCTCCTTAACCACAAAGTCG+GGG | - | 57430:28015-28034 | None:intergenic | 50.0% | |
| CCTTGAATGGCCAAGCATCA+GGG | - | 57430:28912-28931 | None:intergenic | 50.0% | |
| CGCGAATGACCGTACATATC+AGG | - | 57430:28796-28815 | None:intergenic | 50.0% | |
| GAAGAAGTGGTGAAGGCAGA+AGG | - | 57430:27872-27891 | None:intergenic | 50.0% | |
| GCATCGAAGAGTCGCAAGAA+GGG | - | 57430:27943-27962 | None:intergenic | 50.0% | |
| GGAACACTCTGTGCATCAAC+AGG | - | 57430:28937-28956 | None:intergenic | 50.0% | |
| GGCTGAACTGTTGACAGAAC+CGG | - | 57430:27975-27994 | None:intergenic | 50.0% | |
| GTGGATGGAAGAAGTGGTGA+AGG | - | 57430:27879-27898 | None:intergenic | 50.0% | |
| TATGATCCGTCGGCAAAGAC+AGG | + | 57430:28627-28646 | MS.gene34823:CDS | 50.0% | |
| TCCTTGAATGGCCAAGCATC+AGG | - | 57430:28913-28932 | None:intergenic | 50.0% | |
| TGCATCGAAGAGTCGCAAGA+AGG | - | 57430:27944-27963 | None:intergenic | 50.0% | |
| TGCATGATCACCCTGATGCT+TGG | + | 57430:28899-28918 | MS.gene34823:CDS | 50.0% | |
| TGTCCCCGACTTTGTGGTTA+AGG | + | 57430:28009-28028 | MS.gene34823:CDS | 50.0% | |
| TTGGAGAGAGATCGATGGGA+AGG | + | 57430:28526-28545 | MS.gene34823:CDS | 50.0% | |
| ! | ATCCCTGGTTTGAAAGAGGC+TGG | + | 57430:28763-28782 | MS.gene34823:CDS | 50.0% |
| ! | CTGGTTTGAAAGAGGCTGGA+TGG | + | 57430:28767-28786 | MS.gene34823:CDS | 50.0% |
| ! | GTTGATGCACAGAGTGTTCC+TGG | + | 57430:28937-28956 | MS.gene34823:CDS | 50.0% |
| !! | TCGCGGTTTAGAGCTTTGGA+TGG | + | 57430:28811-28830 | MS.gene34823:CDS | 50.0% |
| AACAGTTCAGCCGCCACTTC+CGG | + | 57430:27983-28002 | MS.gene34823:CDS | 55.0% | |
| AAGAGGTGGCTACAGTGCCA+GGG | + | 57430:28072-28091 | MS.gene34823:CDS | 55.0% | |
| ACGCCTCCAACAACGCGATT+AGG | - | 57430:28295-28314 | None:intergenic | 55.0% | |
| AGGGAGCCTAATCGCGTTGT+TGG | + | 57430:28286-28305 | MS.gene34823:CDS | 55.0% | |
| CACAAAGTCGGGGACAGAAC+CGG | - | 57430:28005-28024 | None:intergenic | 55.0% | |
| CCCCGACTTTGTGGTTAAGG+AGG | + | 57430:28012-28031 | MS.gene34823:CDS | 55.0% | |
| CCCTGATGCTTGGCCATTCA+AGG | + | 57430:28909-28928 | MS.gene34823:CDS | 55.0% | |
| GAGCCTAATCGCGTTGTTGG+AGG | + | 57430:28289-28308 | MS.gene34823:CDS | 55.0% | |
| GAGTCGCAAGAAGGGTGGAA+TGG | - | 57430:27935-27954 | None:intergenic | 55.0% | |
| GCGCTTGTGGATGGAAGAAG+TGG | - | 57430:27885-27904 | None:intergenic | 55.0% | |
| GTGACAACACTCCTCCAGTC+TGG | + | 57430:29156-29175 | MS.gene34823:CDS | 55.0% | |
| TCGAAGAGTCGCAAGAAGGG+TGG | - | 57430:27940-27959 | None:intergenic | 55.0% | |
| TCGCAAGAAGGGTGGAATGG+AGG | - | 57430:27932-27951 | None:intergenic | 55.0% | |
| CAAGAGGTGGCTACAGTGCC+AGG | + | 57430:28071-28090 | MS.gene34823:CDS | 60.0% | |
| CGGTTCTGTCCCCGACTTTG+TGG | + | 57430:28003-28022 | MS.gene34823:CDS | 60.0% | |
| ! | TGGTGAAGGCAGAAGGGCAC+TGG | - | 57430:27865-27884 | None:intergenic | 60.0% |
| !! | GGTGAAGGCAGAAGGGCACT+GGG | - | 57430:27864-27883 | None:intergenic | 60.0% |
| GGGGACAGAACCGGAAGTGG+CGG | - | 57430:27996-28015 | None:intergenic | 65.0% | |
| GTCGGGGACAGAACCGGAAG+TGG | - | 57430:27999-28018 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57430 | gene | 27854 | 29194 | 27854 | ID=MS.gene34823 |
| 57430 | mRNA | 27854 | 29194 | 27854 | ID=MS.gene34823.t1;Parent=MS.gene34823 |
| 57430 | exon | 27854 | 29194 | 27854 | ID=MS.gene34823.t1.exon1;Parent=MS.gene34823.t1 |
| 57430 | CDS | 27854 | 29194 | 27854 | ID=cds.MS.gene34823.t1;Parent=MS.gene34823.t1 |
| Gene Sequence |
| Protein sequence |
