AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34922

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34922.t1 MTR_2g019190 58.947 95 28 3 14 99 41 133 1.64e-30 105
MS.gene34922.t1 MTR_8g107300 58.621 87 23 3 16 98 4 81 2.08e-26 93.6
MS.gene34922.t1 MTR_8g107300 57.303 89 25 3 14 98 37 116 3.56e-26 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34922.t1 AT1G30135 43.617 94 48 3 9 99 37 128 1.60e-14 64.7

Find 19 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTGATGCCACTGAGATTC+AGG 0.303605 4.1:-16182461 MS.gene34922:intron
GAATCCGATTCTTCCTCATT+TGG 0.352446 4.1:+16180399 None:intergenic
TTCTCCAAATGAGGAAGAAT+CGG 0.418344 4.1:-16180403 MS.gene34922:CDS
TCTTCTCATTTATCTTAAGA+TGG 0.423225 4.1:+16180360 None:intergenic
TCATTTATCTTAAGATGGTA+AGG 0.430837 4.1:+16180365 None:intergenic
GAAATGGTGAGGACACCAAC+TGG 0.450322 4.1:-16180512 MS.gene34922:CDS
AAGATCATAAGCAGCAGTAC+AGG 0.470194 4.1:-16182519 MS.gene34922:CDS
TGCTGCTTATGATCTTCTTG+TGG 0.500195 4.1:+16182526 None:intergenic
TGCAGAGGTTTCTCCAAATG+AGG 0.508298 4.1:-16180412 MS.gene34922:CDS
CCGTTGACCATTGTCTATGA+TGG 0.521959 4.1:-16182496 MS.gene34922:CDS
CATTTATCTTAAGATGGTAA+GGG 0.531668 4.1:+16180366 None:intergenic
TGCATGGAAAGAGAAGTACT+AGG 0.548727 4.1:+16180443 None:intergenic
TGCAAGCATTAAAATGGACC+TGG 0.550717 4.1:+16180541 None:intergenic
GCATGGAAAGAGAAGTACTA+GGG 0.573020 4.1:+16180444 None:intergenic
ATTTATCTTAAGATGGTAAG+GGG 0.589050 4.1:+16180367 None:intergenic
AATCTTGCCATCATAGACAA+TGG 0.643141 4.1:+16182489 None:intergenic
CCATCATAGACAATGGTCAA+CGG 0.652322 4.1:+16182496 None:intergenic
GGCTCTGATGATGATCCAGT+TGG 0.655026 4.1:+16180497 None:intergenic
TGAACACACCTGAATCTCAG+TGG 0.730872 4.1:+16182453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAATTTTAATATATTA+AGG + chr4.1:16183554-16183573 None:intergenic 0.0%
!!! TTTTTTTATTTTTTAAAATA+TGG - chr4.1:16182454-16182473 MS.gene34922:intron 0.0%
!! ATAATAGTTTGAATTAAAAT+TGG - chr4.1:16183682-16183701 MS.gene34922:intron 10.0%
!!! AAGTAAAAAAACAATTTTTT+AGG + chr4.1:16182278-16182297 None:intergenic 10.0%
!! AAATAAAACAAAAACCTAAT+TGG + chr4.1:16181685-16181704 None:intergenic 15.0%
!! CATTGAATAAATAATGTATA+TGG - chr4.1:16182214-16182233 MS.gene34922:intron 15.0%
!! GAAAAAAAAATCAAAAAACA+TGG + chr4.1:16183498-16183517 None:intergenic 15.0%
!!! ATATACGTTTTTTATTTTTC+TGG + chr4.1:16182325-16182344 None:intergenic 15.0%
!!! ATTAGGTTTTTGTTTTATTT+TGG - chr4.1:16181685-16181704 MS.gene34922:intron 15.0%
!!! ATTTTCTAAGAAATTAAAGA+TGG + chr4.1:16180489-16180508 None:intergenic 15.0%
!!! CTATTTATTAATTTTTTCTG+CGG - chr4.1:16183046-16183065 MS.gene34922:intron 15.0%
!!! TATGCAATTTTTGTTTTTTT+AGG + chr4.1:16182031-16182050 None:intergenic 15.0%
!!! TCTTTAATTTCTTAGAAAAT+TGG - chr4.1:16180489-16180508 MS.gene34922:CDS 15.0%
!!! TTTTTACTTATAAATGTGAA+CGG - chr4.1:16182289-16182308 MS.gene34922:intron 15.0%
!! AGCTACAAATTTAGTAATAA+AGG - chr4.1:16183263-16183282 MS.gene34922:intron 20.0%
!! ATAATATTGGCAAAATTACT+TGG + chr4.1:16180396-16180415 None:intergenic 20.0%
!! ATATCTATGCTCTATATATA+TGG + chr4.1:16181149-16181168 None:intergenic 20.0%
!! ATTAAAAGAGAGAAGATAAA+TGG + chr4.1:16182611-16182630 None:intergenic 20.0%
!! CAATGATATTAGTACTTAAT+AGG + chr4.1:16183095-16183114 None:intergenic 20.0%
!! CATAGAATAATTAATGTATC+TGG + chr4.1:16182253-16182272 None:intergenic 20.0%
!! GATATAAATAAATCAGTAAG+CGG - chr4.1:16183350-16183369 MS.gene34922:intron 20.0%
!! GCTACAAATTTAGTAATAAA+GGG - chr4.1:16183264-16183283 MS.gene34922:intron 20.0%
!! TAAAATTGGAATTAAGTAGT+GGG - chr4.1:16183696-16183715 MS.gene34922:intron 20.0%
!! TAATATTGGCAAAATTACTT+GGG + chr4.1:16180395-16180414 None:intergenic 20.0%
!! TATAAATGCAAACTACAAAT+TGG - chr4.1:16183296-16183315 MS.gene34922:intron 20.0%
!! TGGTGCATATATTATATATA+TGG + chr4.1:16180469-16180488 None:intergenic 20.0%
!! TTAAAATTGGAATTAAGTAG+TGG - chr4.1:16183695-16183714 MS.gene34922:intron 20.0%
!!! AATTTGCTTATAAATGTGAA+CGG + chr4.1:16181968-16181987 None:intergenic 20.0%
!!! ACTTTATTTCACATATAACA+AGG + chr4.1:16181632-16181651 None:intergenic 20.0%
!!! ATTTGTAGTTTGCATTTATA+AGG + chr4.1:16183296-16183315 None:intergenic 20.0%
!!! TAAGCAAAACACAATTTTTA+AGG - chr4.1:16182189-16182208 MS.gene34922:intron 20.0%
!!! TCAAACTATTATTGATGATA+AGG + chr4.1:16183674-16183693 None:intergenic 20.0%
!!! TCTTTTCATCATATAATCTT+TGG + chr4.1:16183230-16183249 None:intergenic 20.0%
!!! TTTTTTTGCAAGCATTAAAA+TGG + chr4.1:16183759-16183778 None:intergenic 20.0%
! AAAATTGGAATTAAGTAGTG+GGG - chr4.1:16183697-16183716 MS.gene34922:intron 25.0%
! AAAGTTAACATGTACTACTT+GGG - chr4.1:16181647-16181666 MS.gene34922:intron 25.0%
! AAATTGCATATAAATGTGAC+CGG - chr4.1:16182041-16182060 MS.gene34922:intron 25.0%
! AATGCTTGCAAAAAAAGAAA+TGG - chr4.1:16183763-16183782 MS.gene34922:intron 25.0%
! ACAAGGAAAATGAAAATATG+GGG + chr4.1:16181352-16181371 None:intergenic 25.0%
! ATGCATTATTGTACATGATA+TGG + chr4.1:16182149-16182168 None:intergenic 25.0%
! ATGTGAATTGTATATATTGC+AGG - chr4.1:16183727-16183746 MS.gene34922:intron 25.0%
! ATTTATCTTAAGATGGTAAG+GGG + chr4.1:16183927-16183946 None:intergenic 25.0%
! CACAAGGAAAATGAAAATAT+GGG + chr4.1:16181353-16181372 None:intergenic 25.0%
! CATTTATCTTAAGATGGTAA+GGG + chr4.1:16183928-16183947 None:intergenic 25.0%
! CTATACAAGTTAAAAACTGA+TGG + chr4.1:16183827-16183846 None:intergenic 25.0%
! GTGCATGTATCATAAAAATT+CGG + chr4.1:16180447-16180466 None:intergenic 25.0%
! TAAAGTTAACATGTACTACT+TGG - chr4.1:16181646-16181665 MS.gene34922:intron 25.0%
! TCATCTGATCATAATAAAGT+TGG - chr4.1:16180658-16180677 MS.gene34922:intron 25.0%
! TCATTTATCTTAAGATGGTA+AGG + chr4.1:16183929-16183948 None:intergenic 25.0%
! TCTTCTCATTTATCTTAAGA+TGG + chr4.1:16183934-16183953 None:intergenic 25.0%
! TGCATGTATCATAAAAATTC+GGG + chr4.1:16180446-16180465 None:intergenic 25.0%
! TGTGTACAATTTATTACGTA+TGG + chr4.1:16182977-16182996 None:intergenic 25.0%
! TTAAACATAAAAATGGTCCT+AGG - chr4.1:16181114-16181133 MS.gene34922:intron 25.0%
! TTGAGAGTTAGTATTTAATG+AGG + chr4.1:16181013-16181032 None:intergenic 25.0%
! TTTATATCTTTCGTTTGTCT+TGG + chr4.1:16183338-16183357 None:intergenic 25.0%
!! ACAACATAACACTTCATTTT+TGG + chr4.1:16183165-16183184 None:intergenic 25.0%
!! ATTTGTTGACCTCAAAATTT+GGG - chr4.1:16183437-16183456 MS.gene34922:intron 25.0%
!! TATTAATTCTGGTGCTAAAT+AGG + chr4.1:16182867-16182886 None:intergenic 25.0%
!! TTTTCAAAAAATGCATATGC+AGG - chr4.1:16182369-16182388 MS.gene34922:intron 25.0%
!!! GATCATAATAAAGTTGGTAT+AGG - chr4.1:16180664-16180683 MS.gene34922:intron 25.0%
AAAGAAGAAGAAGAAGAAGA+AGG - chr4.1:16181735-16181754 MS.gene34922:intron 30.0%
AATAGCATCATCATCTAGTT+AGG - chr4.1:16183574-16183593 MS.gene34922:intron 30.0%
ACAAGAATTAAAGAGAGAGA+GGG + chr4.1:16182951-16182970 None:intergenic 30.0%
ACATTGCATTTATATCTACC+TGG + chr4.1:16180625-16180644 None:intergenic 30.0%
AGAGATTCAGAAATTACTCA+AGG - chr4.1:16182678-16182697 MS.gene34922:intron 30.0%
ATACACGTAATTAAAGCACA+AGG + chr4.1:16181369-16181388 None:intergenic 30.0%
CAAGGAAAATGAAAATATGG+GGG + chr4.1:16181351-16181370 None:intergenic 30.0%
CAATTCTTATCACAACTATG+TGG - chr4.1:16180933-16180952 MS.gene34922:intron 30.0%
GAAGAAGAAGAATTTGAACT+TGG - chr4.1:16181535-16181554 MS.gene34922:intron 30.0%
GATAATAAGTCATTGATGCA+CGG + chr4.1:16181054-16181073 None:intergenic 30.0%
GCACAAGGAAAATGAAAATA+TGG + chr4.1:16181354-16181373 None:intergenic 30.0%
TCTCTCTCTTTAATTCTTGT+GGG - chr4.1:16182951-16182970 MS.gene34922:intron 30.0%
TTGAATGAAAGTTTGGTTGA+GGG + chr4.1:16182574-16182593 None:intergenic 30.0%
TTGCAAAAAAAGAAATGGTG+AGG - chr4.1:16183768-16183787 MS.gene34922:intron 30.0%
! AAGCATTTTCTTTCCACAAT+GGG + chr4.1:16182896-16182915 None:intergenic 30.0%
! AATAGTTAGAGCATTCACAA+TGG - chr4.1:16182830-16182849 MS.gene34922:intron 30.0%
! ATGAAGTGTTATGTTGTAGA+TGG - chr4.1:16183168-16183187 MS.gene34922:intron 30.0%
! CATTTGTTGACCTCAAAATT+TGG - chr4.1:16183436-16183455 MS.gene34922:intron 30.0%
! CGGATTACTCAAAAAACTTT+TGG + chr4.1:16182503-16182522 None:intergenic 30.0%
! GTTTTGCTTATAAATGTGAC+CGG + chr4.1:16182179-16182198 None:intergenic 30.0%
! TAAGGTGAGTTGAAAAAGAA+TGG + chr4.1:16183536-16183555 None:intergenic 30.0%
! TATAAGAAGAAAAGTGTGAG+TGG + chr4.1:16181411-16181430 None:intergenic 30.0%
! TTAAAAACTGATGGTGAAGA+TGG + chr4.1:16183818-16183837 None:intergenic 30.0%
!!! ATATATGGTTTTGTCAACCT+AGG + chr4.1:16181134-16181153 None:intergenic 30.0%
!!! CAAATTTTGAGGTCAACAAA+TGG + chr4.1:16183438-16183457 None:intergenic 30.0%
!!! GTTTTTTGAGTAATCCGTTT+TGG - chr4.1:16182506-16182525 MS.gene34922:CDS 30.0%
!!! TTCATTTTTGGTGCTTAAGT+GGG + chr4.1:16183153-16183172 None:intergenic 30.0%
AATCTTGCCATCATAGACAA+TGG + chr4.1:16181805-16181824 None:intergenic 35.0%
AATTGTATATATTGCAGGCC+AGG - chr4.1:16183732-16183751 MS.gene34922:intron 35.0%
ACGATTCTCTCCATTATGTT+CGG - chr4.1:16180735-16180754 MS.gene34922:intron 35.0%
AGCAAACACAATACTGAGTT+TGG - chr4.1:16180877-16180896 MS.gene34922:intron 35.0%
AGGAGATGAGAATAAGTTTC+GGG - chr4.1:16180525-16180544 MS.gene34922:CDS 35.0%
AGTTTAAAAACTACTCCCTC+CGG + chr4.1:16182063-16182082 None:intergenic 35.0%
CAAATATCAGAAATGGAAGG+AGG + chr4.1:16181575-16181594 None:intergenic 35.0%
CACAAGAATTAAAGAGAGAG+AGG + chr4.1:16182952-16182971 None:intergenic 35.0%
CATTGTGGAAAGAAAATGCT+TGG - chr4.1:16182895-16182914 MS.gene34922:intron 35.0%
CTCTAAATCAACTACCTACA+AGG + chr4.1:16182545-16182564 None:intergenic 35.0%
CTCTCTCTCTTTAATTCTTG+TGG - chr4.1:16182950-16182969 MS.gene34922:intron 35.0%
CTTGAATGAAAGTTTGGTTG+AGG + chr4.1:16182575-16182594 None:intergenic 35.0%
GAGGAGATGAGAATAAGTTT+CGG - chr4.1:16180524-16180543 MS.gene34922:CDS 35.0%
GGACAAATATCAGAAATGGA+AGG + chr4.1:16181578-16181597 None:intergenic 35.0%
GGGGGTGAAAATTAAAAAGT+GGG + chr4.1:16181333-16181352 None:intergenic 35.0%
GGGGTGAAAATTAAAAAGTG+GGG + chr4.1:16181332-16181351 None:intergenic 35.0%
GGGTGAAAATTAAAAAGTGG+GGG + chr4.1:16181331-16181350 None:intergenic 35.0%
TCTAACTCAACCGAACATAA+TGG + chr4.1:16180748-16180767 None:intergenic 35.0%
TCTCTTGAGTGATCACAATA+TGG + chr4.1:16183649-16183668 None:intergenic 35.0%
TCTTTCTTCCAAGAGAGTTA+TGG + chr4.1:16181514-16181533 None:intergenic 35.0%
TGAGAGCTTGAATGAAAGTT+TGG + chr4.1:16182581-16182600 None:intergenic 35.0%
TGGGGGTGAAAATTAAAAAG+TGG + chr4.1:16181334-16181353 None:intergenic 35.0%
TTCTCCAAATGAGGAAGAAT+CGG - chr4.1:16183888-16183907 MS.gene34922:intron 35.0%
TTGTCTTAGACTCTTAGTTG+AGG + chr4.1:16180566-16180585 None:intergenic 35.0%
! AGCATTTTCTTTCCACAATG+GGG + chr4.1:16182895-16182914 None:intergenic 35.0%
! ATAGTTGTGCCCAAATTTTG+AGG + chr4.1:16183449-16183468 None:intergenic 35.0%
! CAAGCATTTTCTTTCCACAA+TGG + chr4.1:16182897-16182916 None:intergenic 35.0%
! CTTGTAGGTAGTTGATTTAG+AGG - chr4.1:16182543-16182562 MS.gene34922:intron 35.0%
!! CAATAAACAGTGAAGTGTTG+TGG + chr4.1:16181486-16181505 None:intergenic 35.0%
!! CACATGTAGTGATTTTGAAG+CGG - chr4.1:16181169-16181188 MS.gene34922:intron 35.0%
!!! CTTCATTTTTGGTGCTTAAG+TGG + chr4.1:16183154-16183173 None:intergenic 35.0%
!!! TACCACTGTTTTAGAACCAA+GGG + chr4.1:16180839-16180858 None:intergenic 35.0%
!!! TTAATTTTTTCTGCGGTGCT+AGG - chr4.1:16183053-16183072 MS.gene34922:intron 35.0%
AAAGTGGGGGAGAGAAATTA+AGG + chr4.1:16181318-16181337 None:intergenic 40.0%
AAGATCATAAGCAGCAGTAC+AGG - chr4.1:16181772-16181791 MS.gene34922:intron 40.0%
AATAAGTCATTGATGCACGG+CGG + chr4.1:16181051-16181070 None:intergenic 40.0%
ACAAATGGATGCAGTGCATT+TGG + chr4.1:16183423-16183442 None:intergenic 40.0%
ACAATAATGCATACTCCCTC+CGG - chr4.1:16182157-16182176 MS.gene34922:intron 40.0%
ACAGTGGTAGACACTTTCAA+TGG - chr4.1:16180850-16180869 MS.gene34922:intron 40.0%
ACTATGTGGTTGAGTTTAGC+CGG - chr4.1:16180947-16180966 MS.gene34922:intron 40.0%
AGATGAAGAAGATGCTTACG+TGG + chr4.1:16181600-16181619 None:intergenic 40.0%
AGCATCATCATCTAGTTAGG+AGG - chr4.1:16183577-16183596 MS.gene34922:intron 40.0%
ATTAATAAGCACCCCCATTG+TGG - chr4.1:16182880-16182899 MS.gene34922:intron 40.0%
CCAACTTCACTTCATTCACA+AGG + chr4.1:16183392-16183411 None:intergenic 40.0%
CCATCATAGACAATGGTCAA+CGG + chr4.1:16181798-16181817 None:intergenic 40.0%
CCTTGTGAATGAAGTGAAGT+TGG - chr4.1:16183389-16183408 MS.gene34922:intron 40.0%
CGTGGGACAAATATCAGAAA+TGG + chr4.1:16181582-16181601 None:intergenic 40.0%
GAATCCGATTCTTCCTCATT+TGG + chr4.1:16183895-16183914 None:intergenic 40.0%
GAGAACAGATCTCTCCAAAA+CGG + chr4.1:16182523-16182542 None:intergenic 40.0%
GATGAAGAAGATGCTTACGT+GGG + chr4.1:16181599-16181618 None:intergenic 40.0%
GATGATATGGATTTCTCCCT+TGG - chr4.1:16180820-16180839 MS.gene34922:intron 40.0%
GCATTTATATCTACCTGGTG+CGG + chr4.1:16180620-16180639 None:intergenic 40.0%
GGAAATACGTGGTTCCAATT+AGG - chr4.1:16181668-16181687 MS.gene34922:intron 40.0%
TCAAGGAGAGTTTGATCAAG+AGG - chr4.1:16182695-16182714 MS.gene34922:intron 40.0%
TCTTAGTTGAGGTTGCTCTT+CGG + chr4.1:16180555-16180574 None:intergenic 40.0%
TGCAAGCATTAAAATGGACC+TGG + chr4.1:16183753-16183772 None:intergenic 40.0%
TGCATATAAATGTGACCGGA+GGG - chr4.1:16182045-16182064 MS.gene34922:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG + chr4.1:16182175-16182194 None:intergenic 40.0%
TGTACTACTTGGGAAATACG+TGG - chr4.1:16181657-16181676 MS.gene34922:intron 40.0%
TTGCATATAAATGTGACCGG+AGG - chr4.1:16182044-16182063 MS.gene34922:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr4.1:16182176-16182195 None:intergenic 40.0%
TTGTCACTCCATAACTCTCT+TGG - chr4.1:16181503-16181522 MS.gene34922:intron 40.0%
! AGTTAGAGCATTCACAATGG+TGG - chr4.1:16182833-16182852 MS.gene34922:intron 40.0%
! GCATTTTCTTTCCACAATGG+GGG + chr4.1:16182894-16182913 None:intergenic 40.0%
! GTTAGAGCATTCACAATGGT+GGG - chr4.1:16182834-16182853 MS.gene34922:intron 40.0%
! TGAGTTGGGCTTTGATGATA+TGG - chr4.1:16180807-16180826 MS.gene34922:intron 40.0%
! TGCTGCTTATGATCTTCTTG+TGG + chr4.1:16181768-16181787 None:intergenic 40.0%
! TTGGTGCTTACAGAGAAATG+TGG - chr4.1:16182914-16182933 MS.gene34922:intron 40.0%
!! AAACAGTGAAGTGTTGTGGA+CGG + chr4.1:16181482-16181501 None:intergenic 40.0%
!! ATGGGGGTGCTTATTAATTC+TGG + chr4.1:16182878-16182897 None:intergenic 40.0%
!! GCATGGAAAGAGAAGTACTA+GGG + chr4.1:16183850-16183869 None:intergenic 40.0%
!! TGCATGGAAAGAGAAGTACT+AGG + chr4.1:16183851-16183870 None:intergenic 40.0%
!!! AACTTTTGGTGACGTTCAAG+AGG + chr4.1:16182489-16182508 None:intergenic 40.0%
!!! CTACCACTGTTTTAGAACCA+AGG + chr4.1:16180840-16180859 None:intergenic 40.0%
ACCATACACACGTTCATCCA+TGG - chr4.1:16180587-16180606 MS.gene34922:intron 45.0%
ATAAGTCATTGATGCACGGC+GGG + chr4.1:16181050-16181069 None:intergenic 45.0%
CCATGCAAAAATCGTTGCAG+AGG - chr4.1:16183864-16183883 MS.gene34922:intron 45.0%
CCGTTGACCATTGTCTATGA+TGG - chr4.1:16181795-16181814 MS.gene34922:intron 45.0%
CGATCTCATTTGTTTGCCTC+CGG + chr4.1:16180969-16180988 None:intergenic 45.0%
GAGAGATCTGTTCTCCTTGT+AGG - chr4.1:16182528-16182547 MS.gene34922:CDS 45.0%
GTGGTAGACACTTTCAATGG+TGG - chr4.1:16180853-16180872 MS.gene34922:intron 45.0%
GTGTGCATTCGTGATTCAGA+TGG - chr4.1:16180688-16180707 MS.gene34922:intron 45.0%
TGAACACACCTGAATCTCAG+TGG + chr4.1:16181841-16181860 None:intergenic 45.0%
TGCAGAGGTTTCTCCAAATG+AGG - chr4.1:16183879-16183898 MS.gene34922:intron 45.0%
TGCAGGTCTTACTACACATG+TGG - chr4.1:16182386-16182405 MS.gene34922:intron 45.0%
! AGAATCGTGTTGTTCTCGCA+AGG + chr4.1:16180723-16180742 None:intergenic 45.0%
! CCTCTGCAACGATTTTTGCA+TGG + chr4.1:16183867-16183886 None:intergenic 45.0%
!! CTCCCTTGGTTCTAAAACAG+TGG - chr4.1:16180834-16180853 MS.gene34922:intron 45.0%
!!! TTTTTTCTGCGGTGCTAGGT+TGG - chr4.1:16183057-16183076 MS.gene34922:intron 45.0%
!! TTAAGAAAAAAATATAATAT+TGG + chr4.1:16180409-16180428 None:intergenic 5.0%
!!! AATTTTTTTAAACATAAAAA+TGG - chr4.1:16181107-16181126 MS.gene34922:intron 5.0%
!!! TGTTTAAAAAAATTTTTTTA+AGG + chr4.1:16181101-16181120 None:intergenic 5.0%
AGAAAATTGGAAGCTCGCGC+AGG - chr4.1:16180502-16180521 MS.gene34922:CDS 50.0%
ATGTGGTTGAGTTTAGCCGG+AGG - chr4.1:16180950-16180969 MS.gene34922:intron 50.0%
GAAATGGTGAGGACACCAAC+TGG - chr4.1:16183779-16183798 MS.gene34922:intron 50.0%
GACTGATGCCACTGAGATTC+AGG - chr4.1:16181830-16181849 MS.gene34922:intron 50.0%
GCCATGGATGAACGTGTGTA+TGG + chr4.1:16180591-16180610 None:intergenic 50.0%
! CGGTGCTAGGTTGGAGAAAT+TGG - chr4.1:16183066-16183085 MS.gene34922:intron 50.0%
! GGCTCTGATGATGATCCAGT+TGG + chr4.1:16183797-16183816 None:intergenic 50.0%
! TTCTCAAGCGTTGCAGTGAG+CGG - chr4.1:16180783-16180802 MS.gene34922:intron 50.0%
AAATTGGAAGCTCGCGCAGG+AGG - chr4.1:16180505-16180524 MS.gene34922:CDS 55.0%
TGACGACGCTCAGCATACTG+CGG + chr4.1:16181213-16181232 None:intergenic 55.0%
TTGCAGTGAGCGGCTGAGTT+GGG - chr4.1:16180793-16180812 MS.gene34922:intron 55.0%
ACGACGCTCAGCATACTGCG+GGG + chr4.1:16181211-16181230 None:intergenic 60.0%
CCATGGCAGATAACCGCACC+AGG - chr4.1:16180604-16180623 MS.gene34922:intron 60.0%
CCTGGTGCGGTTATCTGCCA+TGG + chr4.1:16180607-16180626 None:intergenic 60.0%
GACGACGCTCAGCATACTGC+GGG + chr4.1:16181212-16181231 None:intergenic 60.0%
GTTGCAGTGAGCGGCTGAGT+TGG - chr4.1:16180792-16180811 MS.gene34922:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 16180355 16183958 16180355 ID=MS.gene34922
chr4.1 mRNA 16180355 16183958 16180355 ID=MS.gene34922.t1;Parent=MS.gene34922
chr4.1 exon 16183938 16183958 16183938 ID=MS.gene34922.t1.exon1;Parent=MS.gene34922.t1
chr4.1 CDS 16183938 16183958 16183938 ID=cds.MS.gene34922.t1;Parent=MS.gene34922.t1
chr4.1 exon 16182462 16182551 16182462 ID=MS.gene34922.t1.exon2;Parent=MS.gene34922.t1
chr4.1 CDS 16182462 16182551 16182462 ID=cds.MS.gene34922.t1;Parent=MS.gene34922.t1
chr4.1 exon 16180355 16180564 16180355 ID=MS.gene34922.t1.exon3;Parent=MS.gene34922.t1
chr4.1 CDS 16180355 16180564 16180355 ID=cds.MS.gene34922.t1;Parent=MS.gene34922.t1
Gene Sequence

>MS.gene34922

ATGATGATCAAATCATTTCATGTAAGTTATGAGACTACCCAAGTAATTTTGCCAATATTATATTTTTTTCTTAAAAACTAATTTGAGTCCCGAATTTTTATGATACATGCACCATATATATAATATATGCACCATCTTTAATTTCTTAGAAAATTGGAAGCTCGCGCAGGAGGAGATGAGAATAAGTTTCGGGACAACCGAAGAGCAACCTCAACTAAGAGTCTAAGACAAAACCATACACACGTTCATCCATGGCAGATAACCGCACCAGGTAGATATAAATGCAATGTTGATGCTTCTTTGTCATCTGATCATAATAAAGTTGGTATAGGAGTGTGCATTCGTGATTCAGATGGCAGCTTTGTCCTTGCGAGAACAACACGATTCTCTCCATTATGTTCGGTTGAGTTAGAAGAAACATTGAGTCTTTCTCAAGCGTTGCAGTGAGCGGCTGAGTTGGGCTTTGATGATATGGATTTCTCCCTTGGTTCTAAAACAGTGGTAGACACTTTCAATGGTGGAAGCAAACACAATACTGAGTTTGGTAGTATTATTCAGCATTGTAGACATGCTTTTAACAATTCTTATCACAACTATGTGGTTGAGTTTAGCCGGAGGCAAACAAATGAGATCGCTCATGAATTAACACATACAACCTCATTAAATACTAACTCTCAAATGTTTATTAACGTCCCGCCGTGCATCAATGACTTATTATCTAATGAAATGCTATGAATTTATTTCCTTAAAAAAATTTTTTTAAACATAAAAATGGTCCTAGGTTGACAAAACCATATATATAGAGCATAGATATCACATGTAGTGATTTTGAAGCGGCGCTATTAAAGTCTGTCCCCGCAGTATGCTGAGCGTCGTCATTAATAAATCACATACTCGTGAAACATGTGCTCAAATTAAATGAGAAAATAAATACAACACTTCTAACTAACACTTCGTTTTCCTTAATTTCTCTCCCCCACTTTTTAATTTTCACCCCCATATTTTCATTTTCCTTGTGCTTTAATTACGTGTATTGCAAGTGCTTATAATATTCCACTCACACTTTTCTTCTTATATATAGTGTAGTATTAGTGATGTATCACTAACGCTTCTTAATACTTTCTCCGTCCACAACACTTCACTGTTTATTGTCACTCCATAACTCTCTTGGAAGAAAGATGAAGAAGAAGAATTTGAACTTGGAACTTCGTCTTTTTCCTCCTTCCATTTCTGATATTTGTCCCACGTAAGCATCTTCTTCATCTCTTTTTTTTCCTTGTTATATGTGAAATAAAGTTAACATGTACTACTTGGGAAATACGTGGTTCCAATTAGGTTTTTGTTTTATTTTGGTTATTTTTGTTTGTTCAATGAAGAATGAAAGAAGAAGAAGAAGAAGAAGGTGAGAGTCCACAAGAAGATCATAAGCAGCAGTACAGGCCGTTGACCATTGTCTATGATGGCAAGATTTCTGTGACTGATGCCACTGAGATTCAGGTGTGTTCATGATTGTTCAATTTTCTGATTTTGAAAATAAAATAACTGAGATCTCTTCTTTTTTGTTTTTACTTTAATAATGTATTGTATTATTTGCTAATCTTATACTCACTCCGTTCACATTTATAAGCAAATTTTACTTTTTCAGATTCTAGATACATTACTTATTTAATGAACCTAAAAAAACAAAAATTGCATATAAATGTGACCGGAGGGAGTAGTTTTTAAACTACTATATATCTCTACAAATTATCTATGTGAACATGAAAAAAAGAATAACTAATGATTAATAAACCATATCATGTACAATAATGCATACTCCCTCCGGTCACATTTATAAGCAAAACACAATTTTTAAGGTTCATTGAATAAATAATGTATATGGTGTATAAATGTGACCAGATACATTAATTATTCTATGAACCTAAAAAATTGTTTTTTTACTTATAAATGTGAACGGAGAAAGTACACCAGAAAAATAAAAAACGTATATAGCTAGCTTTGTATTTATTTTCTCTTTTCAAAAAATGCATATGCAGGTCTTACTACACATGTGGTTAAGAATTCATACTTAGATTTTCATCTCTCAAAATATGTTTTTATTTTTTTATTTTTTAAAATATGGTTGTTTAATCCTCTTGAACGTCACCAAAAGTTTTTTGAGTAATCCGTTTTGGAGAGATCTGTTCTCCTTGTAGGTAGTTGATTTAGAGGTGATTCCCTCAACCAAACTTTCATTCAAGCTCTCATTCATCTCCATTTATCTTCTCTCTTTTAATTAAGAGAGAAATAAAGAGATTGATTTTATAAAGAAAGAAAGAAATAAAGAGATTCAGAAATTACTCAAGGAGAGTTTGATCAAGAGGATCAACTATTGTTGACTCTTTTATCATCATGAAACAAATTTTTATGAAGCATGCATTTAGATTTTCTATACAATTAAAGAGATTAATTATATATAACTAAAATTTTAAATTAAATAGTTAGAGCATTCACAATGGTGGGTTCTTCACCTATTTAGCACCAGAATTAATAAGCACCCCCATTGTGGAAAGAAAATGCTTGGTGCTTACAGAGAAATGTGGCTCTATAATCACCCTCTCTCTCTTTAATTCTTGTGGGCCATACGTAATAAATTGTACACAAAACAATAAACTTTGTTGAAATACAATGACATTGAGTTATTAATATGATCTATTTATTAATTTTTTCTGCGGTGCTAGGTTGGAGAAATTGGATGCCTATTAAGTACTAATATCATTGAAAATTAAAAAAATAGATGATGTGTACATGCATGACCCACTTAAGCACCAAAAATGAAGTGTTATGTTGTAGATGGTCATGTAAAGTTAATTTTTACTACTAATAATCAAGACCAAAGATTATATGATGAAAAGACAAGTGAGAGAAAAGCTACAAATTTAGTAATAAAGGGAAAATTCCTTATAAATGCAAACTACAAATTGGTGATAGTTAAGCAAGACCAAGACAAACGAAAGATATAAATAAATCAGTAAGCGGTCTACATCTGAACTGTCCTTGTGAATGAAGTGAAGTTGGCTAAATTTCCAAATGCACTGCATCCATTTGTTGACCTCAAAATTTGGGCACAACTATATATATACACACATTTCTATATATATCCATGTTTTTTGATTTTTTTTTCTTTCTTTCTCAAGAGCCATTCTTTTTCAACTCACCTTAATATATTAAAATTAAAATAATAGCATCATCATCTAGTTAGGAGGCACGTAGCTAGACACTATTTTATTATAGCTCTTCAATTTTCACGTGCCATATTGTGATCACTCAAGAGATTCCTTATCATCAATAATAGTTTGAATTAAAATTGGAATTAAGTAGTGGGGTAATATAATGTGAATTGTATATATTGCAGGCCAGGTCCATTTTAATGCTTGCAAAAAAAGAAATGGTGAGGACACCAACTGGATCATCATCAGAGCCATCTTCACCATCAGTTTTTAACTTGTATAGCCCTAGTACTTCTCTTTCCATGCAAAAATCGTTGCAGAGGTTTCTCCAAATGAGGAAGAATCGGATTCAAGAAGCATCCCCTTACCATCTTAAGATAAATGAGAAGAATTAA
Protein sequence

>MS.gene34922.t1

MMIKSFHSPQEDHKQQYRPLTIVYDGKISVTDATEIQARSILMLAKKEMVRTPTGSSSEPSSPSVFNLYSPSTSLSMQKSLQRFLQMRKNRIQEASPYHLKINEKN