Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34994.t1 | PNX80761.1 | 57.1 | 217 | 77 | 6 | 51 | 260 | 17 | 224 | 7.20E-55 | 224.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34994.t1 | A0A2K3LQE0 | 57.1 | 217 | 77 | 6 | 51 | 260 | 17 | 224 | 5.2e-55 | 224.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene34994.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34994.t1 | MTR_3g080590 | 59.200 | 125 | 47 | 3 | 7 | 130 | 31 | 152 | 5.14e-34 | 120 |
| MS.gene34994.t1 | MTR_7g446520 | 37.778 | 180 | 101 | 3 | 83 | 261 | 87 | 256 | 9.60e-29 | 111 |
| MS.gene34994.t1 | MTR_7g446510 | 45.045 | 111 | 54 | 2 | 157 | 261 | 155 | 264 | 1.85e-20 | 89.4 |
| MS.gene34994.t1 | MTR_7g446480 | 44.144 | 111 | 55 | 2 | 157 | 261 | 140 | 249 | 5.54e-20 | 87.8 |
| MS.gene34994.t1 | MTR_7g028855 | 39.041 | 146 | 78 | 5 | 83 | 218 | 119 | 263 | 1.89e-19 | 87.4 |
| MS.gene34994.t1 | MTR_1g070355 | 26.786 | 168 | 112 | 4 | 80 | 237 | 70 | 236 | 8.62e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTACTTGGGTTTATTAAAA+AGG | 0.237474 | 4.4:+18327432 | MS.gene34994:CDS |
| TCCCTCAATTTACGATAATT+AGG | 0.249777 | 4.4:-18327316 | None:intergenic |
| TAATAAACCCAAGTACTTCA+TGG | 0.324697 | 4.4:-18327426 | None:intergenic |
| AAACGTGCAAGAGCTAATTC+AGG | 0.401835 | 4.4:-18327280 | None:intergenic |
| AAAGGACTTGCAAAATTTCA+AGG | 0.409145 | 4.4:+18327450 | MS.gene34994:CDS |
| TTCGAATGGTTGTCCTAGAT+TGG | 0.412474 | 4.4:-18327189 | None:intergenic |
| CACTATGCATTTGTAACCAT+TGG | 0.416660 | 4.4:-18326964 | None:intergenic |
| TGAAGAATGACAACCAATCT+AGG | 0.416853 | 4.4:+18327176 | MS.gene34994:CDS |
| AAACATTACATAAGACTTTG+TGG | 0.418547 | 4.4:-18327041 | None:intergenic |
| TTTCACTTCATTCTTTCTTA+CGG | 0.439411 | 4.4:-18327083 | None:intergenic |
| TTCCTAATTATCGTAAATTG+AGG | 0.443566 | 4.4:+18327314 | MS.gene34994:CDS |
| AAGGGTGGCCATGAAGTACT+TGG | 0.450370 | 4.4:+18327418 | MS.gene34994:CDS |
| CGCACAACAATTATTTCGAA+TGG | 0.477990 | 4.4:-18327203 | None:intergenic |
| CAATTAGTATGCAATACATA+TGG | 0.480421 | 4.4:+18327133 | MS.gene34994:CDS |
| GTCACAACATGTCATATCTT+CGG | 0.481439 | 4.4:+18327379 | MS.gene34994:CDS |
| TGACAGATGATGATGATGTA+AGG | 0.507309 | 4.4:+18326990 | MS.gene34994:CDS |
| GTCTACGACTTCGTAAGTAT+TGG | 0.517520 | 4.4:+18327238 | MS.gene34994:CDS |
| AACGTGCAAGAGCTAATTCA+GGG | 0.539372 | 4.4:-18327279 | None:intergenic |
| AATACATATGGTGTTAATGA+TGG | 0.546002 | 4.4:+18327145 | MS.gene34994:CDS |
| CGTACTGATAGAGATGTAGA+TGG | 0.548996 | 4.4:+18326887 | MS.gene34994:CDS |
| GTGGAATTCAACCATGGTAA+TGG | 0.562978 | 4.4:+18326911 | MS.gene34994:CDS |
| TCCTAATTATCGTAAATTGA+GGG | 0.567960 | 4.4:+18327315 | MS.gene34994:CDS |
| ATTTCAAGGATCATCAAGCA+AGG | 0.575712 | 4.4:+18327464 | MS.gene34994:CDS |
| AGGGTGGCCATGAAGTACTT+GGG | 0.595891 | 4.4:+18327419 | MS.gene34994:CDS |
| GCTTCAGTATCACTATCTAT+CGG | 0.596147 | 4.4:-18326767 | None:intergenic |
| GCTTCAGTATCACTATCTAT+CGG | 0.596147 | 4.4:-18326836 | None:intergenic |
| AATGGTGTTTGCAGTGAACA+AGG | 0.604546 | 4.4:+18326929 | MS.gene34994:CDS |
| TGATAGAGATGTAGATGGTG+TGG | 0.606468 | 4.4:+18326892 | MS.gene34994:CDS |
| GCTTTACCTTGGAAGTAACT+CGG | 0.617155 | 4.4:-18327520 | None:intergenic |
| CACTGCAAACACCATTACCA+TGG | 0.617401 | 4.4:-18326922 | None:intergenic |
| GCTACTCCGAGTTACTTCCA+AGG | 0.624648 | 4.4:+18327514 | MS.gene34994:CDS |
| TTTCAAGGATCATCAAGCAA+GGG | 0.627896 | 4.4:+18327465 | MS.gene34994:CDS |
| GACAGATGATGATGATGTAA+GGG | 0.635954 | 4.4:+18326991 | MS.gene34994:CDS |
| GCTGATAAACAAGTTGCTAG+TGG | 0.641318 | 4.4:+18327340 | MS.gene34994:CDS |
| AAGGGAAAGAGAAACTCCAA+TGG | 0.674525 | 4.4:+18326948 | MS.gene34994:CDS |
| GGAAATGAAATTCAACACAG+TGG | 0.694737 | 4.4:+18327012 | MS.gene34994:CDS |
| ATGGTGTTTGCAGTGAACAA+GGG | 0.699949 | 4.4:+18326930 | MS.gene34994:CDS |
| GCTAGTGGTCTACATTCACA+AGG | 0.709355 | 4.4:+18327355 | MS.gene34994:CDS |
| GATGGTGTGGAATTCAACCA+TGG | 0.744654 | 4.4:+18326905 | MS.gene34994:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTTACATTTACAAATGA+TGG | + | chr4.4:18327568-18327587 | MS.gene34994:intron | 20.0% |
| !! | ATTCTGATGTATCAAATAAT+AGG | + | chr4.4:18328047-18328066 | MS.gene34994:intron | 20.0% |
| !!! | GGTTTTAAATTTGTTTTCAA+AGG | + | chr4.4:18327793-18327812 | MS.gene34994:intron | 20.0% |
| ! | AAACAATATAGACATACATG+TGG | + | chr4.4:18327772-18327791 | MS.gene34994:intron | 25.0% |
| ! | AAACATTACATAAGACTTTG+TGG | - | chr4.4:18327044-18327063 | None:intergenic | 25.0% |
| ! | AATTATGCAAGAAATCCAAA+AGG | - | chr4.4:18328213-18328232 | None:intergenic | 25.0% |
| ! | ACTTATAGAAATAACAGGTA+TGG | + | chr4.4:18327673-18327692 | MS.gene34994:intron | 25.0% |
| ! | AGTACTTGGGTTTATTAAAA+AGG | + | chr4.4:18327432-18327451 | MS.gene34994:CDS | 25.0% |
| ! | CAATTAGTATGCAATACATA+TGG | + | chr4.4:18327133-18327152 | MS.gene34994:CDS | 25.0% |
| ! | TCACTATGATGTAAAAACAA+TGG | - | chr4.4:18328372-18328391 | None:intergenic | 25.0% |
| ! | TCCTAATTATCGTAAATTGA+GGG | + | chr4.4:18327315-18327334 | MS.gene34994:CDS | 25.0% |
| ! | TGATGTATCAAATAATAGGT+GGG | + | chr4.4:18328051-18328070 | MS.gene34994:intron | 25.0% |
| ! | TTATATCCACTAAAAATGCA+CGG | - | chr4.4:18327705-18327724 | None:intergenic | 25.0% |
| ! | TTCCTAATTATCGTAAATTG+AGG | + | chr4.4:18327314-18327333 | MS.gene34994:CDS | 25.0% |
| ! | TTTCACTTCATTCTTTCTTA+CGG | - | chr4.4:18327086-18327105 | None:intergenic | 25.0% |
| !! | AATACATATGGTGTTAATGA+TGG | + | chr4.4:18327145-18327164 | MS.gene34994:CDS | 25.0% |
| !! | CAAAAATAATTGTTTCTCCA+TGG | - | chr4.4:18327731-18327750 | None:intergenic | 25.0% |
| !! | CATTCAAATTCAAACTTTTC+GGG | - | chr4.4:18328010-18328029 | None:intergenic | 25.0% |
| !! | CCATTCAAATTCAAACTTTT+CGG | - | chr4.4:18328011-18328030 | None:intergenic | 25.0% |
| !! | TTTTGGATGTTTGTCATAAA+TGG | - | chr4.4:18327819-18327838 | None:intergenic | 25.0% |
| !!! | ATACCTGTTATTTCTATAAG+TGG | - | chr4.4:18327674-18327693 | None:intergenic | 25.0% |
| AAAGGACTTGCAAAATTTCA+AGG | + | chr4.4:18327450-18327469 | MS.gene34994:CDS | 30.0% | |
| AAGTTGTGTTTCCATATACA+AGG | + | chr4.4:18327882-18327901 | MS.gene34994:intron | 30.0% | |
| ACACCACTTATAGAAATAAC+AGG | + | chr4.4:18327668-18327687 | MS.gene34994:intron | 30.0% | |
| AGAACGACATTAATCTATGA+AGG | + | chr4.4:18328145-18328164 | MS.gene34994:intron | 30.0% | |
| ATCTGCCCAAAAATATTGTT+TGG | - | chr4.4:18327598-18327617 | None:intergenic | 30.0% | |
| ATTATTTCGATGCAAATTCC+AGG | - | chr4.4:18327921-18327940 | None:intergenic | 30.0% | |
| CCGAAAAGTTTGAATTTGAA+TGG | + | chr4.4:18328008-18328027 | MS.gene34994:intron | 30.0% | |
| CTGATGTATCAAATAATAGG+TGG | + | chr4.4:18328050-18328069 | MS.gene34994:intron | 30.0% | |
| GTAAAAACAATGGAGACAAA+GGG | - | chr4.4:18328362-18328381 | None:intergenic | 30.0% | |
| GTTTGAATTTGAATGGAAGA+TGG | + | chr4.4:18328015-18328034 | MS.gene34994:intron | 30.0% | |
| TAATAAACCCAAGTACTTCA+TGG | - | chr4.4:18327429-18327448 | None:intergenic | 30.0% | |
| TATATCCACTAAAAATGCAC+GGG | - | chr4.4:18327704-18327723 | None:intergenic | 30.0% | |
| TCCCTCAATTTACGATAATT+AGG | - | chr4.4:18327319-18327338 | None:intergenic | 30.0% | |
| TCTGCCCAAAAATATTGTTT+GGG | - | chr4.4:18327597-18327616 | None:intergenic | 30.0% | |
| TCTTGCCTATACCAAAATAT+GGG | + | chr4.4:18328328-18328347 | MS.gene34994:intron | 30.0% | |
| TGTAAAAACAATGGAGACAA+AGG | - | chr4.4:18328363-18328382 | None:intergenic | 30.0% | |
| TTATGACAAACATCCAAAAG+CGG | + | chr4.4:18327820-18327839 | MS.gene34994:intron | 30.0% | |
| ! | AAATAATTGTTTCTCCATGG+AGG | - | chr4.4:18327728-18327747 | None:intergenic | 30.0% |
| ! | AAATGAAACTTATCGGTTGA+CGG | + | chr4.4:18328075-18328094 | MS.gene34994:intron | 30.0% |
| !!! | CATATCTTCGGTTTTTTGAT+GGG | + | chr4.4:18327391-18327410 | MS.gene34994:CDS | 30.0% |
| !!! | CCAAAAGGTTGTTTTTATGT+CGG | - | chr4.4:18328198-18328217 | None:intergenic | 30.0% |
| !!! | TCATATCTTCGGTTTTTTGA+TGG | + | chr4.4:18327390-18327409 | MS.gene34994:CDS | 30.0% |
| AATCAGTATCAGCTTTACCT+TGG | - | chr4.4:18327534-18327553 | None:intergenic | 35.0% | |
| ATTTCAAGGATCATCAAGCA+AGG | + | chr4.4:18327464-18327483 | MS.gene34994:CDS | 35.0% | |
| CACTATGCATTTGTAACCAT+TGG | - | chr4.4:18326967-18326986 | None:intergenic | 35.0% | |
| CGCACAACAATTATTTCGAA+TGG | - | chr4.4:18327206-18327225 | None:intergenic | 35.0% | |
| CTTGCCTATACCAAAATATG+GGG | + | chr4.4:18328329-18328348 | MS.gene34994:intron | 35.0% | |
| GACATTAATCTATGAAGGAG+TGG | + | chr4.4:18328150-18328169 | MS.gene34994:intron | 35.0% | |
| GCTTCAGTATCACTATCTAT+CGG | - | chr4.4:18326770-18326789 | None:intergenic | 35.0% | |
| GGAAATGAAATTCAACACAG+TGG | + | chr4.4:18327012-18327031 | MS.gene34994:CDS | 35.0% | |
| GGTGGGTAAATGAAACTTAT+CGG | + | chr4.4:18328068-18328087 | MS.gene34994:intron | 35.0% | |
| GTCACAACATGTCATATCTT+CGG | + | chr4.4:18327379-18327398 | MS.gene34994:CDS | 35.0% | |
| GTCTTGCCTATACCAAAATA+TGG | + | chr4.4:18328327-18328346 | MS.gene34994:intron | 35.0% | |
| TGAAGAATGACAACCAATCT+AGG | + | chr4.4:18327176-18327195 | MS.gene34994:CDS | 35.0% | |
| TTTCAAGGATCATCAAGCAA+GGG | + | chr4.4:18327465-18327484 | MS.gene34994:CDS | 35.0% | |
| ! | AATGAAACTTATCGGTTGAC+GGG | + | chr4.4:18328076-18328095 | MS.gene34994:intron | 35.0% |
| ! | CCGACATAAAAACAACCTTT+TGG | + | chr4.4:18328195-18328214 | MS.gene34994:intron | 35.0% |
| ! | GACAGATGATGATGATGTAA+GGG | + | chr4.4:18326991-18327010 | MS.gene34994:CDS | 35.0% |
| ! | GACGCCCAAACAATATTTTT+GGG | + | chr4.4:18327590-18327609 | MS.gene34994:intron | 35.0% |
| ! | TGACAGATGATGATGATGTA+AGG | + | chr4.4:18326990-18327009 | MS.gene34994:CDS | 35.0% |
| ! | TTTAGTGGATATAACCTCCA+TGG | + | chr4.4:18327711-18327730 | MS.gene34994:intron | 35.0% |
| !! | AAAATCCCGTGCATTTTTAG+TGG | + | chr4.4:18327696-18327715 | MS.gene34994:intron | 35.0% |
| !!! | AACACCCCATATTTTGGTAT+AGG | - | chr4.4:18328336-18328355 | None:intergenic | 35.0% |
| !!! | ATGCGAGAACATTTTTTGTG+TGG | + | chr4.4:18328118-18328137 | MS.gene34994:intron | 35.0% |
| !!! | GGTTTTTTGATGGGTCAAAA+GGG | + | chr4.4:18327400-18327419 | MS.gene34994:CDS | 35.0% |
| !!! | TATTTTTGGGCAGATTCTAC+TGG | + | chr4.4:18327603-18327622 | MS.gene34994:intron | 35.0% |
| !!! | TCAAACTTTTCGGGTGTATA+AGG | - | chr4.4:18328001-18328020 | None:intergenic | 35.0% |
| AAAACAATGGAGACAAAGGG+TGG | - | chr4.4:18328359-18328378 | None:intergenic | 40.0% | |
| AAACGTGCAAGAGCTAATTC+AGG | - | chr4.4:18327283-18327302 | None:intergenic | 40.0% | |
| AACGTGCAAGAGCTAATTCA+GGG | - | chr4.4:18327282-18327301 | None:intergenic | 40.0% | |
| AAGGGAAAGAGAAACTCCAA+TGG | + | chr4.4:18326948-18326967 | MS.gene34994:CDS | 40.0% | |
| AATGGTGTTTGCAGTGAACA+AGG | + | chr4.4:18326929-18326948 | MS.gene34994:CDS | 40.0% | |
| ATGGTGTTTGCAGTGAACAA+GGG | + | chr4.4:18326930-18326949 | MS.gene34994:CDS | 40.0% | |
| GCTGATAAACAAGTTGCTAG+TGG | + | chr4.4:18327340-18327359 | MS.gene34994:CDS | 40.0% | |
| GCTTTACCTTGGAAGTAACT+CGG | - | chr4.4:18327523-18327542 | None:intergenic | 40.0% | |
| GTCTACGACTTCGTAAGTAT+TGG | + | chr4.4:18327238-18327257 | MS.gene34994:CDS | 40.0% | |
| GTGGAATTCAACCATGGTAA+TGG | + | chr4.4:18326911-18326930 | MS.gene34994:CDS | 40.0% | |
| TGATAGAGATGTAGATGGTG+TGG | + | chr4.4:18326892-18326911 | MS.gene34994:CDS | 40.0% | |
| TTCGAATGGTTGTCCTAGAT+TGG | - | chr4.4:18327192-18327211 | None:intergenic | 40.0% | |
| ! | CGTACTGATAGAGATGTAGA+TGG | + | chr4.4:18326887-18326906 | MS.gene34994:CDS | 40.0% |
| ! | GGACGCCCAAACAATATTTT+TGG | + | chr4.4:18327589-18327608 | MS.gene34994:intron | 40.0% |
| !! | TTTTTGATGGGTCAAAAGGG+TGG | + | chr4.4:18327403-18327422 | MS.gene34994:CDS | 40.0% |
| !!! | CGGTTTTTTGATGGGTCAAA+AGG | + | chr4.4:18327399-18327418 | MS.gene34994:CDS | 40.0% |
| CAAAGGCGATGCCTTGTATA+TGG | - | chr4.4:18327896-18327915 | None:intergenic | 45.0% | |
| CACTGCAAACACCATTACCA+TGG | - | chr4.4:18326925-18326944 | None:intergenic | 45.0% | |
| CCAAAAGCGGTGATTACTGA+TGG | + | chr4.4:18327833-18327852 | MS.gene34994:intron | 45.0% | |
| GATGGTGTGGAATTCAACCA+TGG | + | chr4.4:18326905-18326924 | MS.gene34994:CDS | 45.0% | |
| GCTAGTGGTCTACATTCACA+AGG | + | chr4.4:18327355-18327374 | MS.gene34994:CDS | 45.0% | |
| GGCAAGACAGTTGTCAACAT+AGG | - | chr4.4:18328315-18328334 | None:intergenic | 45.0% | |
| TTTGGGCAGATTCTACTGGT+AGG | + | chr4.4:18327607-18327626 | MS.gene34994:intron | 45.0% | |
| ! | CCATCAGTAATCACCGCTTT+TGG | - | chr4.4:18327836-18327855 | None:intergenic | 45.0% |
| ! | TTATCGGTTGACGGGAATGT+GGG | + | chr4.4:18328084-18328103 | MS.gene34994:intron | 45.0% |
| GATGCAAATTCCAGGCGCAA+AGG | - | chr4.4:18327913-18327932 | None:intergenic | 50.0% | |
| GCTACTCCGAGTTACTTCCA+AGG | + | chr4.4:18327514-18327533 | MS.gene34994:CDS | 50.0% | |
| ! | AAGGGTGGCCATGAAGTACT+TGG | + | chr4.4:18327418-18327437 | MS.gene34994:CDS | 50.0% |
| ! | AGGGTGGCCATGAAGTACTT+GGG | + | chr4.4:18327419-18327438 | MS.gene34994:CDS | 50.0% |
| ! | CTTATCGGTTGACGGGAATG+TGG | + | chr4.4:18328083-18328102 | MS.gene34994:intron | 50.0% |
| ! | GGGTGGAACACCCCATATTT+TGG | - | chr4.4:18328342-18328361 | None:intergenic | 50.0% |
| !! | CAAGGCATCGCCTTTGCGCC+TGG | + | chr4.4:18327900-18327919 | MS.gene34994:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18326755 | 18328396 | 18326755 | ID=MS.gene34994 |
| chr4.4 | mRNA | 18326755 | 18328396 | 18326755 | ID=MS.gene34994.t1;Parent=MS.gene34994 |
| chr4.4 | exon | 18326755 | 18327535 | 18326755 | ID=MS.gene34994.t1.exon1;Parent=MS.gene34994.t1 |
| chr4.4 | CDS | 18326755 | 18327535 | 18326755 | ID=cds.MS.gene34994.t1;Parent=MS.gene34994.t1 |
| chr4.4 | exon | 18328389 | 18328396 | 18328389 | ID=MS.gene34994.t1.exon2;Parent=MS.gene34994.t1 |
| chr4.4 | CDS | 18328389 | 18328396 | 18328389 | ID=cds.MS.gene34994.t1;Parent=MS.gene34994.t1 |
| Gene Sequence |
| Protein sequence |
