Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34995.t1 | PNX80761.1 | 59.6 | 161 | 57 | 3 | 67 | 225 | 32 | 186 | 2.40E-44 | 189.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34995.t1 | Q9LKR4 | 34.9 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.0e-11 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34995.t1 | A0A2K3LQE0 | 59.6 | 161 | 57 | 3 | 67 | 225 | 32 | 186 | 1.8e-44 | 189.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene34995.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34995.t1 | MTR_3g080590 | 65.161 | 155 | 32 | 2 | 1 | 133 | 1 | 155 | 5.10e-62 | 192 |
| MS.gene34995.t1 | MTR_7g446520 | 36.646 | 161 | 86 | 3 | 78 | 237 | 83 | 228 | 1.46e-27 | 108 |
| MS.gene34995.t1 | MTR_7g028855 | 40.789 | 152 | 81 | 3 | 80 | 224 | 117 | 266 | 1.88e-27 | 109 |
| MS.gene34995.t1 | MTR_5g076070 | 24.091 | 220 | 146 | 5 | 23 | 237 | 8 | 211 | 2.54e-15 | 73.9 |
| MS.gene34995.t1 | MTR_8g035530 | 41.667 | 72 | 41 | 1 | 72 | 142 | 2 | 73 | 3.74e-13 | 63.5 |
| MS.gene34995.t1 | MTR_1g070355 | 28.148 | 135 | 92 | 3 | 83 | 212 | 74 | 208 | 8.11e-13 | 67.8 |
| MS.gene34995.t1 | MTR_6g022080 | 24.390 | 164 | 118 | 3 | 80 | 238 | 82 | 244 | 1.40e-12 | 65.9 |
| MS.gene34995.t1 | MTR_7g446510 | 40.964 | 83 | 47 | 2 | 156 | 237 | 155 | 236 | 4.11e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34995.t1 | AT5G28530 | 34.884 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.33e-14 | 73.6 |
| MS.gene34995.t1 | AT5G28530 | 34.884 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.39e-14 | 73.2 |
| MS.gene34995.t1 | AT5G28530 | 34.884 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.39e-14 | 73.2 |
| MS.gene34995.t1 | AT5G28530 | 34.884 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.63e-14 | 73.2 |
| MS.gene34995.t1 | AT5G28530 | 34.884 | 129 | 78 | 3 | 89 | 211 | 61 | 189 | 1.83e-14 | 72.8 |
Find 39 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTCAATTTCCGGTAATT+AGG | 0.200610 | 4.4:+18329937 | None:intergenic |
| TATCAACTTCACTCAATTTC+CGG | 0.238375 | 4.4:+18329929 | None:intergenic |
| ACAACTTTGTTTGTAAGAAT+TGG | 0.289038 | 4.4:-18330023 | MS.gene34995:CDS |
| TTCATTTGATTCCTAATTAC+CGG | 0.300050 | 4.4:-18329948 | MS.gene34995:CDS |
| AGAGCTTGGGTTTATCAAAA+AGG | 0.330351 | 4.4:-18329819 | MS.gene34995:CDS |
| GTGAAGATATAGAAGTCAAT+AGG | 0.343295 | 4.4:-18330407 | MS.gene34995:CDS |
| TGATAAACCCAAGCTCTTCA+TGG | 0.346825 | 4.4:+18329825 | None:intergenic |
| TTCACGGATCATCAAGCAAA+AGG | 0.380013 | 4.4:-18329785 | MS.gene34995:CDS |
| TGAAGATATAGAAGTCAATA+GGG | 0.403531 | 4.4:-18330406 | MS.gene34995:CDS |
| ATAAGAATGGCAGCCGATCT+AGG | 0.405495 | 4.4:-18330089 | MS.gene34995:CDS |
| TGTACATAGTGATACAAATA+AGG | 0.406690 | 4.4:-18330472 | MS.gene34995:CDS |
| TGTCAGAGCATGTCATATCT+TGG | 0.421443 | 4.4:-18329875 | MS.gene34995:CDS |
| GTCAGAGCATGTCATATCTT+GGG | 0.459621 | 4.4:-18329874 | MS.gene34995:CDS |
| AAAGGACTTGTATAATTTCA+CGG | 0.466531 | 4.4:-18329801 | MS.gene34995:CDS |
| AACGCGCTAGAGCTAATTCA+TGG | 0.466898 | 4.4:+18329974 | None:intergenic |
| CGCACAACAGTTAGTTCGAA+TGG | 0.478548 | 4.4:+18330062 | None:intergenic |
| TTCGAATGGTTGTCCTAGAT+CGG | 0.479891 | 4.4:+18330076 | None:intergenic |
| AGGGTGGCCATGAAGAGCTT+GGG | 0.480186 | 4.4:-18329832 | MS.gene34995:CDS |
| GTGATGTGACAGATGATGTA+AGG | 0.502812 | 4.4:-18330275 | MS.gene34995:CDS |
| AAGGGTGGCCATGAAGAGCT+TGG | 0.503025 | 4.4:-18329833 | MS.gene34995:CDS |
| CTCTGACACCTTGTGAATGC+AGG | 0.503685 | 4.4:+18329890 | None:intergenic |
| TCAACTAGTATGCAATAAAG+AGG | 0.542921 | 4.4:-18330130 | MS.gene34995:CDS |
| TCACGGATCATCAAGCAAAA+GGG | 0.549571 | 4.4:-18329784 | MS.gene34995:CDS |
| GTGGAATTCAACCATGGTAA+TGG | 0.562978 | 4.4:-18330348 | MS.gene34995:CDS |
| GTTAGTGACCTGCATTCACA+AGG | 0.600956 | 4.4:-18329898 | MS.gene34995:CDS |
| ACTGATGCATTTGTAACCAT+CGG | 0.601265 | 4.4:+18330296 | None:intergenic |
| AATGGTGTTTGCAGTGAGCA+AGG | 0.608644 | 4.4:-18330330 | MS.gene34995:CDS |
| ATGGTGTTTGCAGTGAGCAA+GGG | 0.627698 | 4.4:-18330329 | MS.gene34995:CDS |
| CAACTAGTATGCAATAAAGA+GGG | 0.632289 | 4.4:-18330129 | MS.gene34995:CDS |
| TAATAGAGATGCAGACGGTG+TGG | 0.636745 | 4.4:-18330367 | MS.gene34995:CDS |
| CACTGCAAACACCATTACCA+TGG | 0.645481 | 4.4:+18330337 | None:intergenic |
| CAAGGGAAAGAGAACTCCGA+TGG | 0.649967 | 4.4:-18330312 | MS.gene34995:CDS |
| ACATAGTGATACAAATAAGG+TGG | 0.650304 | 4.4:-18330469 | MS.gene34995:CDS |
| GAAGATATAGAAGTCAATAG+GGG | 0.650650 | 4.4:-18330405 | MS.gene34995:CDS |
| TGATGTGACAGATGATGTAA+GGG | 0.654823 | 4.4:-18330274 | MS.gene34995:CDS |
| GTATCACTATGTACATCAAG+AGG | 0.658850 | 4.4:+18330480 | None:intergenic |
| GACGGTGTGGAATTCAACCA+TGG | 0.691057 | 4.4:-18330354 | MS.gene34995:CDS |
| CGTACTAATAGAGATGCAGA+CGG | 0.718664 | 4.4:-18330372 | MS.gene34995:CDS |
| GGATATAGAATTCAACACAG+AGG | 0.752405 | 4.4:-18330253 | MS.gene34995:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATGAAAAAAATAAGAA+TGG | - | chr4.4:18330185-18330204 | MS.gene34995:CDS | 15.0% |
| !!! | ATATCTTGGGTTTTTTTTTA+TGG | - | chr4.4:18330426-18330445 | MS.gene34995:CDS | 20.0% |
| !!! | TATCTTGGGTTTTTTTTTAT+GGG | - | chr4.4:18330427-18330446 | MS.gene34995:CDS | 20.0% |
| !!! | TTTTTTTTTATGGGTGAAAA+GGG | - | chr4.4:18330436-18330455 | MS.gene34995:CDS | 20.0% |
| ! | AAAGGACTTGTATAATTTCA+CGG | - | chr4.4:18330486-18330505 | MS.gene34995:CDS | 25.0% |
| ! | TGAAGATATAGAAGTCAATA+GGG | - | chr4.4:18329881-18329900 | MS.gene34995:CDS | 25.0% |
| ! | TGTACATAGTGATACAAATA+AGG | - | chr4.4:18329815-18329834 | MS.gene34995:CDS | 25.0% |
| ! | TTCATTTGATTCCTAATTAC+CGG | - | chr4.4:18330339-18330358 | MS.gene34995:CDS | 25.0% |
| !! | ACAACTTTGTTTGTAAGAAT+TGG | - | chr4.4:18330264-18330283 | MS.gene34995:CDS | 25.0% |
| !! | TTTCACTTCATCTTTTCTTA+CGG | + | chr4.4:18330108-18330127 | None:intergenic | 25.0% |
| !!! | GTTTTTTTTTATGGGTGAAA+AGG | - | chr4.4:18330435-18330454 | MS.gene34995:CDS | 25.0% |
| ACATAGTGATACAAATAAGG+TGG | - | chr4.4:18329818-18329837 | MS.gene34995:CDS | 30.0% | |
| CAACTAGTATGCAATAAAGA+GGG | - | chr4.4:18330158-18330177 | MS.gene34995:CDS | 30.0% | |
| GAAGATATAGAAGTCAATAG+GGG | - | chr4.4:18329882-18329901 | MS.gene34995:CDS | 30.0% | |
| GTGAAGATATAGAAGTCAAT+AGG | - | chr4.4:18329880-18329899 | MS.gene34995:CDS | 30.0% | |
| TATCAACTTCACTCAATTTC+CGG | + | chr4.4:18330361-18330380 | None:intergenic | 30.0% | |
| TCAACTAGTATGCAATAAAG+AGG | - | chr4.4:18330157-18330176 | MS.gene34995:CDS | 30.0% | |
| ACTGATGCATTTGTAACCAT+CGG | + | chr4.4:18329994-18330013 | None:intergenic | 35.0% | |
| AGAGCTTGGGTTTATCAAAA+AGG | - | chr4.4:18330468-18330487 | MS.gene34995:CDS | 35.0% | |
| GGATATAGAATTCAACACAG+AGG | - | chr4.4:18330034-18330053 | MS.gene34995:CDS | 35.0% | |
| GTATCACTATGTACATCAAG+AGG | + | chr4.4:18329810-18329829 | None:intergenic | 35.0% | |
| TCACTCAATTTCCGGTAATT+AGG | + | chr4.4:18330353-18330372 | None:intergenic | 35.0% | |
| TGATGTGACAGATGATGTAA+GGG | - | chr4.4:18330013-18330032 | MS.gene34995:CDS | 35.0% | |
| !!! | TTTTTTATGGGTGAAAAGGG+TGG | - | chr4.4:18330439-18330458 | MS.gene34995:CDS | 35.0% |
| CGTACTAATAGAGATGCAGA+CGG | - | chr4.4:18329915-18329934 | MS.gene34995:CDS | 40.0% | |
| GTCAGAGCATGTCATATCTT+GGG | - | chr4.4:18330413-18330432 | MS.gene34995:CDS | 40.0% | |
| GTGATGTGACAGATGATGTA+AGG | - | chr4.4:18330012-18330031 | MS.gene34995:CDS | 40.0% | |
| GTGGAATTCAACCATGGTAA+TGG | - | chr4.4:18329939-18329958 | MS.gene34995:CDS | 40.0% | |
| TCACGGATCATCAAGCAAAA+GGG | - | chr4.4:18330503-18330522 | MS.gene34995:CDS | 40.0% | |
| TGATAAACCCAAGCTCTTCA+TGG | + | chr4.4:18330465-18330484 | None:intergenic | 40.0% | |
| TGTCAGAGCATGTCATATCT+TGG | - | chr4.4:18330412-18330431 | MS.gene34995:CDS | 40.0% | |
| TTCACGGATCATCAAGCAAA+AGG | - | chr4.4:18330502-18330521 | MS.gene34995:CDS | 40.0% | |
| TTCGAATGGTTGTCCTAGAT+CGG | + | chr4.4:18330214-18330233 | None:intergenic | 40.0% | |
| ! | CTTTTCTTACGGCAAATCTG+TGG | + | chr4.4:18330097-18330116 | None:intergenic | 40.0% |
| AACGCGCTAGAGCTAATTCA+TGG | + | chr4.4:18330316-18330335 | None:intergenic | 45.0% | |
| AATGGTGTTTGCAGTGAGCA+AGG | - | chr4.4:18329957-18329976 | MS.gene34995:CDS | 45.0% | |
| ATAAGAATGGCAGCCGATCT+AGG | - | chr4.4:18330198-18330217 | MS.gene34995:CDS | 45.0% | |
| ATGGTGTTTGCAGTGAGCAA+GGG | - | chr4.4:18329958-18329977 | MS.gene34995:CDS | 45.0% | |
| CACTGCAAACACCATTACCA+TGG | + | chr4.4:18329953-18329972 | None:intergenic | 45.0% | |
| CGCACAACAGTTAGTTCGAA+TGG | + | chr4.4:18330228-18330247 | None:intergenic | 45.0% | |
| GTTAGTGACCTGCATTCACA+AGG | - | chr4.4:18330389-18330408 | MS.gene34995:CDS | 45.0% | |
| TAATAGAGATGCAGACGGTG+TGG | - | chr4.4:18329920-18329939 | MS.gene34995:CDS | 45.0% | |
| CAAGGGAAAGAGAACTCCGA+TGG | - | chr4.4:18329975-18329994 | MS.gene34995:CDS | 50.0% | |
| CTCTGACACCTTGTGAATGC+AGG | + | chr4.4:18330400-18330419 | None:intergenic | 50.0% | |
| GACGGTGTGGAATTCAACCA+TGG | - | chr4.4:18329933-18329952 | MS.gene34995:CDS | 50.0% | |
| AAGGGTGGCCATGAAGAGCT+TGG | - | chr4.4:18330454-18330473 | MS.gene34995:CDS | 55.0% | |
| AGGGTGGCCATGAAGAGCTT+GGG | - | chr4.4:18330455-18330474 | MS.gene34995:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18329780 | 18330529 | 18329780 | ID=MS.gene34995 |
| chr4.4 | mRNA | 18329780 | 18330529 | 18329780 | ID=MS.gene34995.t1;Parent=MS.gene34995 |
| chr4.4 | exon | 18329780 | 18330529 | 18329780 | ID=MS.gene34995.t1.exon1;Parent=MS.gene34995.t1 |
| chr4.4 | CDS | 18329780 | 18330529 | 18329780 | ID=cds.MS.gene34995.t1;Parent=MS.gene34995.t1 |
| Gene Sequence |
| Protein sequence |
