AlfalfaGEDB | MS.gene35649

M. sativa cultivar XinJiangDaYe / MS.gene35649

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35649.t1	PNY06273.1	93	43	3	0	1	43	70	112	2.30E-15	90.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35649.t1	Q39008	61.4	57	21	1	1	56	496	552	4.9e-11	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35649.t1	A0A2K3NTC4	93.0	43	3	0	1	43	70	112	1.6e-15	90.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35649.t1	MTR_3g073250	82.222	45	8	0	1	45	446	490	2.97e-21	85.1
MS.gene35649.t1	MTR_3g073250	82.222	45	8	0	1	45	433	477	3.01e-21	85.1
MS.gene35649.t1	MTR_5g059940	66.071	56	19	0	1	56	288	343	7.19e-21	83.6
MS.gene35649.t1	MTR_5g060350	64.286	56	20	0	1	56	484	539	4.78e-20	81.6
MS.gene35649.t1	MTR_0685s0020	66.071	56	19	0	1	56	154	209	8.30e-20	79.3
MS.gene35649.t1	MTR_5g059910	67.857	56	18	0	1	56	294	349	1.60e-19	80.1
MS.gene35649.t1	MTR_5g059880	66.071	56	19	0	1	56	355	410	6.14e-19	78.6
MS.gene35649.t1	MTR_4g094415	74.419	43	11	0	1	43	327	369	4.67e-17	73.2
MS.gene35649.t1	MTR_4g094415	68.889	45	14	0	1	45	327	371	1.32e-15	68.9
MS.gene35649.t1	MTR_3g096230	57.778	45	18	1	1	45	410	453	6.48e-11	55.8
MS.gene35649.t1	MTR_3g096230	57.778	45	18	1	1	45	410	453	6.90e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35649.t1	AT4G08500	61.404	57	21	1	1	56	496	552	1.28e-15	69.3
MS.gene35649.t1	AT4G08480	61.404	57	21	1	1	56	664	720	4.16e-15	67.8
MS.gene35649.t1	AT4G12020	56.897	58	23	1	1	56	1710	1767	1.65e-14	65.9
MS.gene35649.t1	AT4G12020	56.897	58	23	1	1	56	1777	1834	1.73e-14	65.9
MS.gene35649.t1	AT4G12020	56.897	58	23	1	1	56	1785	1842	1.73e-14	65.9
MS.gene35649.t1	AT4G08470	64.444	45	15	1	1	44	466	510	3.99e-13	62.0
MS.gene35649.t1	AT4G08470	64.444	45	15	1	1	44	384	428	4.38e-13	62.0
MS.gene35649.t1	AT4G12020	60.870	46	18	0	11	56	1781	1826	8.96e-13	61.2
MS.gene35649.t1	AT4G12020	60.870	46	18	0	11	56	1773	1818	9.14e-13	61.2

Find 22 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAATTGGAGTATGGAATT+TGG	0.170486	3.3:+63413454	None:intergenic
CCAAATTCCATACTCCAATT+TGG	0.210816	3.3:-63413454	MS.gene35649:CDS
CAAGCTGATGTTTGGAGTTT+GGG	0.235597	3.3:-63413504	MS.gene35649:CDS
TCAAGCTGATGTTTGGAGTT+TGG	0.249389	3.3:-63413505	MS.gene35649:CDS
GGCACATACACATTCCAAAT+TGG	0.357007	3.3:+63413440	None:intergenic
ATGGCCTTCAAGCTGATGTT+TGG	0.373571	3.3:-63413512	MS.gene35649:CDS
ACACATTCCAAATTGGAGTA+TGG	0.390116	3.3:+63413447	None:intergenic
AACTCCAAACATCAGCTTGA+AGG	0.428588	3.3:+63413508	None:intergenic
AAAGGAAAGAACGAAGGTTA+TGG	0.447441	3.3:-63413531	MS.gene35649:CDS
GAGTTTGGGATGCACTGTGC+TGG	0.447799	3.3:-63413490	MS.gene35649:CDS
TGACACCTACAGAAGGTAAT+GGG	0.465640	3.3:+63413397	None:intergenic
ACCTTCTGTAGGTGTCAGTA+AGG	0.468408	3.3:-63413391	MS.gene35649:CDS
ACTGTGCTGGAGATGCTAAC+TGG	0.470469	3.3:-63413477	MS.gene35649:CDS
CTGACACCTACAGAAGGTAA+TGG	0.492606	3.3:+63413396	None:intergenic
TTTCTCCCATTACCTTCTGT+AGG	0.508029	3.3:-63413402	MS.gene35649:CDS
TGGGATATGCAGGTCGTGAA+AGG	0.550867	3.3:-63413549	MS.gene35649:intron
TCTGTAGGTGTCAGTAAGGG+TGG	0.613359	3.3:-63413387	MS.gene35649:CDS
AACATTCTGGATGGCCCCTG+AGG	0.614718	3.3:-63413697	None:intergenic
CCTTCTGTAGGTGTCAGTAA+GGG	0.636753	3.3:-63413390	MS.gene35649:CDS
CCCTTACTGACACCTACAGA+AGG	0.676668	3.3:+63413390	None:intergenic
GTCGTGAAAGGAAAGAACGA+AGG	0.708624	3.3:-63413537	MS.gene35649:CDS
AGATTGAAAGCAAACCTCAG+GGG	0.742082	3.3:+63413683	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTTATTAATTATTAGTC+TGG	-	chr3.3:63413501-63413520	MS.gene35649:CDS	15.0%
!!	GACTAATAATTAATAAACAT+TGG	+	chr3.3:63413501-63413520	None:intergenic	15.0%
!!!	TGTTTATTAATTATTAGTCT+GGG	-	chr3.3:63413502-63413521	MS.gene35649:CDS	15.0%
	GAGAAAAAATAGCAATGACA+TGG	+	chr3.3:63413654-63413673	None:intergenic	30.0%
	TATATCTTGTGTTCATGTTG+AGG	+	chr3.3:63413431-63413450	None:intergenic	30.0%
	AAAGGAAAGAACGAAGGTTA+TGG	-	chr3.3:63413539-63413558	MS.gene35649:CDS	35.0%
	AAGATTGAAAGCAAACCTCA+GGG	+	chr3.3:63413391-63413410	None:intergenic	35.0%
	ACACAAGATATAGCAAAGCT+AGG	-	chr3.3:63413439-63413458	MS.gene35649:CDS	35.0%
	ACACATTCCAAATTGGAGTA+TGG	+	chr3.3:63413626-63413645	None:intergenic	35.0%
	CACAAGATATAGCAAAGCTA+GGG	-	chr3.3:63413440-63413459	MS.gene35649:CDS	35.0%
	CCAAATTCCATACTCCAATT+TGG	-	chr3.3:63413616-63413635	MS.gene35649:intron	35.0%
	CCAAATTGGAGTATGGAATT+TGG	+	chr3.3:63413619-63413638	None:intergenic	35.0%
	TAAGATTGAAAGCAAACCTC+AGG	+	chr3.3:63413392-63413411	None:intergenic	35.0%
!	ATTATTAGTCTGGGATATGC+AGG	-	chr3.3:63413511-63413530	MS.gene35649:CDS	35.0%
	AACTCCAAACATCAGCTTGA+AGG	+	chr3.3:63413565-63413584	None:intergenic	40.0%
	AGATTGAAAGCAAACCTCAG+GGG	+	chr3.3:63413390-63413409	None:intergenic	40.0%
	GGCACATACACATTCCAAAT+TGG	+	chr3.3:63413633-63413652	None:intergenic	40.0%
	TGACACCTACAGAAGGTAAT+GGG	+	chr3.3:63413676-63413695	None:intergenic	40.0%
	TTTCTCCCATTACCTTCTGT+AGG	-	chr3.3:63413668-63413687	MS.gene35649:intron	40.0%
!	CAAGCTGATGTTTGGAGTTT+GGG	-	chr3.3:63413566-63413585	MS.gene35649:intron	40.0%
!	TCAAGCTGATGTTTGGAGTT+TGG	-	chr3.3:63413565-63413584	MS.gene35649:intron	40.0%
	ACCTTCTGTAGGTGTCAGTA+AGG	-	chr3.3:63413679-63413698	MS.gene35649:intron	45.0%
	ATGGCCTTCAAGCTGATGTT+TGG	-	chr3.3:63413558-63413577	MS.gene35649:intron	45.0%
	CCTTCTGTAGGTGTCAGTAA+GGG	-	chr3.3:63413680-63413699	MS.gene35649:intron	45.0%
	CTGACACCTACAGAAGGTAA+TGG	+	chr3.3:63413677-63413696	None:intergenic	45.0%
	GTCGTGAAAGGAAAGAACGA+AGG	-	chr3.3:63413533-63413552	MS.gene35649:CDS	45.0%
	ACTGTGCTGGAGATGCTAAC+TGG	-	chr3.3:63413593-63413612	MS.gene35649:intron	50.0%
	CCCTTACTGACACCTACAGA+AGG	+	chr3.3:63413683-63413702	None:intergenic	50.0%
	TCTGTAGGTGTCAGTAAGGG+TGG	-	chr3.3:63413683-63413702	MS.gene35649:intron	50.0%
	TGGGATATGCAGGTCGTGAA+AGG	-	chr3.3:63413521-63413540	MS.gene35649:CDS	50.0%
!	GAGTTTGGGATGCACTGTGC+TGG	-	chr3.3:63413580-63413599	MS.gene35649:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	63413383	63413709	63413383	ID=MS.gene35649
chr3.3	mRNA	63413383	63413709	63413383	ID=MS.gene35649.t1;Parent=MS.gene35649
chr3.3	exon	63413698	63413709	63413698	ID=MS.gene35649.t1.exon1;Parent=MS.gene35649.t1
chr3.3	CDS	63413698	63413709	63413698	ID=cds.MS.gene35649.t1;Parent=MS.gene35649.t1
chr3.3	exon	63413383	63413559	63413383	ID=MS.gene35649.t1.exon2;Parent=MS.gene35649.t1
chr3.3	CDS	63413383	63413559	63413383	ID=cds.MS.gene35649.t1;Parent=MS.gene35649.t1

Gene Sequence	>MS.gene35649 ATGGCCCCTGAGGTTTGCTTTCAATCTTAACTTTTCAGTATTATACCTCAACATGAACACAAGATATAGCAAAGCTAGGGTTTTTGAGATTGCGTATGAAGAATGAGCTTCTATTCCAATGTTTATTAATTATTAGTCTGGGATATGCAGGTCGTGAAAGGAAAGAACGAAGGTTATGGCCTTCAAGCTGATGTTTGGAGTTTGGGATGCACTGTGCTGGAGATGCTAACTGGCCAAATTCCATACTCCAATTTGGAATGTGTATGTGCCATGTCATTGCTATTTTTTCTCCCATTACCTTCTGTAGGTGTCAGTAAGGGTGGTTAA
Protein sequence	>MS.gene35649.t1 MAPEVVKGKNEGYGLQADVWSLGCTVLEMLTGQIPYSNLECVCAMSLLFFLPLPSVGVSKGG