AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35774

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35774.t1 MTR_2g089100 99.346 459 2 1 1 459 1 458 0.0 940
MS.gene35774.t1 MTR_5g015950 43.448 435 198 8 46 446 28 448 5.60e-120 358
MS.gene35774.t1 MTR_3g053270 43.448 435 198 8 46 446 28 448 5.60e-120 358
MS.gene35774.t1 MTR_4g095500 41.772 474 219 9 13 446 14 470 3.14e-119 357
MS.gene35774.t1 MTR_4g097080 30.049 406 241 8 51 445 131 504 4.61e-59 202
MS.gene35774.t1 MTR_5g015490 31.618 408 250 7 45 445 106 491 4.01e-58 199
MS.gene35774.t1 MTR_2g099110 28.404 426 249 9 41 445 62 452 4.44e-48 171
MS.gene35774.t1 MTR_1g029420 29.798 396 248 7 51 443 223 591 2.43e-47 172
MS.gene35774.t1 MTR_5g094450 25.342 438 297 7 11 443 119 531 3.20e-44 162
MS.gene35774.t1 MTR_7g027190 28.261 414 245 10 47 443 294 672 5.40e-42 158
MS.gene35774.t1 MTR_5g097480 27.295 403 251 8 50 443 174 543 9.74e-42 156
MS.gene35774.t1 MTR_3g089055 27.047 403 250 8 51 443 196 564 7.94e-41 154
MS.gene35774.t1 MTR_3g089055 27.047 403 250 8 51 443 201 569 8.43e-41 154
MS.gene35774.t1 MTR_2g097410 26.521 411 261 9 46 446 364 743 1.10e-39 152
MS.gene35774.t1 MTR_2g082090 25.622 402 259 7 50 443 209 578 4.23e-39 149
MS.gene35774.t1 MTR_4g064160 27.340 406 254 9 50 446 313 686 5.87e-39 150
MS.gene35774.t1 MTR_3g056110 25.708 424 267 10 32 443 154 541 5.80e-38 145
MS.gene35774.t1 MTR_7g062120 28.293 410 245 10 50 446 369 742 6.59e-38 147
MS.gene35774.t1 MTR_4g064150 26.650 409 257 9 50 446 358 735 9.47e-38 146
MS.gene35774.t1 MTR_4g064200 28.288 403 246 9 53 446 284 652 2.17e-37 145
MS.gene35774.t1 MTR_4g064120 26.290 407 261 9 50 446 251 628 2.47e-36 142
MS.gene35774.t1 MTR_2g097310 27.517 447 253 13 26 446 239 640 5.89e-36 140
MS.gene35774.t1 MTR_4g102790 25.616 406 261 10 53 448 292 666 1.54e-35 140
MS.gene35774.t1 MTR_2g097473 26.368 402 254 9 53 446 289 656 1.67e-35 139
MS.gene35774.t1 MTR_4g064180 26.406 409 258 11 50 446 251 628 1.13e-34 137
MS.gene35774.t1 MTR_2g097467 25.935 401 257 9 53 446 289 656 3.14e-34 135
MS.gene35774.t1 MTR_4g133660 26.667 405 254 11 50 444 172 543 3.30e-34 135
MS.gene35774.t1 MTR_5g009080 24.943 441 274 9 47 443 50 477 5.38e-34 133
MS.gene35774.t1 MTR_2g097463 26.866 402 252 9 53 446 290 657 5.53e-34 135
MS.gene35774.t1 MTR_3g022830 25.708 424 267 10 54 443 26 435 1.25e-33 131
MS.gene35774.t1 MTR_3g065980 26.253 419 242 11 47 443 166 539 2.27e-33 132
MS.gene35774.t1 MTR_2g097350 27.073 410 248 10 50 446 271 642 8.21e-32 129
MS.gene35774.t1 MTR_4g104020 25.829 422 260 11 41 443 132 519 5.66e-31 125
MS.gene35774.t1 MTR_2g026250 26.303 403 256 9 50 443 225 595 8.79e-31 125
MS.gene35774.t1 MTR_8g020840 25.728 412 264 11 46 443 164 547 3.05e-30 123
MS.gene35774.t1 MTR_8g442410 26.116 448 282 11 8 443 114 524 7.43e-30 122
MS.gene35774.t1 MTR_4g076140 23.624 436 285 9 47 443 42 468 8.32e-30 121
MS.gene35774.t1 MTR_2g097390 26.098 410 252 9 50 446 272 643 4.99e-29 120
MS.gene35774.t1 MTR_4g076020 24.000 400 252 10 56 452 87 437 9.23e-29 118
MS.gene35774.t1 MTR_1g069725 24.876 402 257 10 47 444 103 463 4.27e-28 116
MS.gene35774.t1 MTR_4g077760 27.068 399 248 13 52 443 192 554 8.96e-26 110
MS.gene35774.t1 MTR_2g097380 25.000 396 225 11 60 446 231 563 3.30e-23 102
MS.gene35774.t1 MTR_7g074650 21.939 392 265 10 56 445 441 793 1.39e-22 101
MS.gene35774.t1 MTR_3g072710 28.571 322 188 11 134 446 214 502 2.22e-22 100
MS.gene35774.t1 MTR_8g093070 23.892 406 253 14 53 447 139 499 2.45e-19 90.9
MS.gene35774.t1 MTR_1g096030 21.718 419 260 12 53 443 80 458 8.92e-18 85.9
MS.gene35774.t1 MTR_7g057230 21.393 402 264 11 54 443 295 656 1.64e-15 79.3
MS.gene35774.t1 MTR_4g026485 23.573 403 246 14 54 443 270 623 3.29e-15 78.6
MS.gene35774.t1 MTR_5g058860 24.818 411 270 13 47 444 113 497 1.11e-14 76.6
MS.gene35774.t1 MTR_0092s0100 23.558 416 231 15 54 443 376 730 5.04e-14 74.7
MS.gene35774.t1 MTR_0092s0100 23.558 416 231 15 54 443 373 727 5.63e-14 74.7
MS.gene35774.t1 MTR_2g034250 20.414 338 227 8 54 379 211 518 5.99e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35774.t1 AT3G49950 43.891 442 205 11 13 445 2 409 7.20e-118 351
MS.gene35774.t1 AT4G37650 29.078 423 252 12 44 446 137 531 1.04e-51 183
MS.gene35774.t1 AT2G04890 27.960 397 243 9 53 443 53 412 5.00e-44 159
MS.gene35774.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene35774.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene35774.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene35774.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene35774.t1 AT1G21450 27.397 438 276 12 16 443 187 592 2.04e-41 155
MS.gene35774.t1 AT1G50600 27.182 401 254 8 50 443 156 525 3.88e-41 154
MS.gene35774.t1 AT1G50600 27.182 401 254 8 50 443 231 600 8.28e-41 154
MS.gene35774.t1 AT1G50600 27.182 401 254 8 50 443 227 596 1.19e-40 154
MS.gene35774.t1 AT1G50420 26.994 489 297 12 3 443 2 478 3.81e-39 147
MS.gene35774.t1 AT5G67411 39.631 217 106 8 171 381 1 198 2.33e-38 138
MS.gene35774.t1 AT1G07520 27.941 408 250 10 53 446 154 531 5.13e-36 140
MS.gene35774.t1 AT1G07520 27.941 408 250 10 53 446 154 531 5.13e-36 140
MS.gene35774.t1 AT1G07520 27.941 408 250 10 53 446 319 696 1.27e-35 140
MS.gene35774.t1 AT1G07520 27.941 408 250 10 53 446 318 695 1.41e-35 140
MS.gene35774.t1 AT2G29065 27.901 405 255 9 53 446 252 630 1.87e-35 139
MS.gene35774.t1 AT3G03450 26.119 402 258 10 47 443 177 544 3.43e-35 137
MS.gene35774.t1 AT4G17230 26.933 401 259 9 50 445 155 526 6.55e-35 137
MS.gene35774.t1 AT4G17230 26.933 401 259 9 50 445 155 526 6.55e-35 137
MS.gene35774.t1 AT1G07530 25.735 408 263 10 48 446 391 767 6.61e-35 138
MS.gene35774.t1 AT2G37650 26.290 407 253 11 50 445 344 714 2.64e-34 136
MS.gene35774.t1 AT2G37650 26.290 407 253 11 50 445 332 702 2.73e-34 136
MS.gene35774.t1 AT5G17490 26.585 410 253 10 47 449 154 522 6.16e-34 134
MS.gene35774.t1 AT2G01570 25.743 404 254 8 46 443 217 580 1.17e-32 130
MS.gene35774.t1 AT2G29060 25.792 442 279 12 18 445 250 656 3.22e-32 130
MS.gene35774.t1 AT2G29060 25.792 442 279 12 18 445 287 693 3.74e-32 130
MS.gene35774.t1 AT1G14920 26.119 402 255 11 46 443 165 528 4.07e-32 129
MS.gene35774.t1 AT1G63100 27.027 370 232 9 53 417 277 613 2.49e-31 127
MS.gene35774.t1 AT1G63100 27.027 370 232 9 53 417 252 588 2.61e-31 127
MS.gene35774.t1 AT1G66350 24.750 400 255 7 47 443 149 505 1.59e-30 124
MS.gene35774.t1 AT5G59450 25.672 409 262 12 50 447 224 601 1.35e-28 119
MS.gene35774.t1 AT3G46600 25.616 406 262 9 50 447 1 374 2.63e-28 115
MS.gene35774.t1 AT5G41920 24.631 406 259 11 46 443 33 399 2.77e-28 116
MS.gene35774.t1 AT3G46600 25.616 406 262 9 50 447 177 550 1.38e-27 115
MS.gene35774.t1 AT3G46600 25.616 406 262 9 50 447 209 582 2.39e-27 115
MS.gene35774.t1 AT5G66770 27.596 366 225 12 52 412 219 549 9.28e-24 104
MS.gene35774.t1 AT3G54220 23.704 405 265 11 46 445 286 651 1.41e-23 104
MS.gene35774.t1 AT3G13840 24.638 414 257 11 43 443 138 509 3.03e-23 102
MS.gene35774.t1 AT5G52510 22.359 407 273 8 49 443 264 639 3.42e-21 97.1
MS.gene35774.t1 AT1G55580 24.009 429 266 12 50 443 41 444 3.81e-19 90.1
MS.gene35774.t1 AT4G08250 25.648 386 246 12 68 444 124 477 1.88e-16 82.0
MS.gene35774.t1 AT3G50650 22.642 371 241 10 84 443 206 541 4.22e-15 78.2
MS.gene35774.t1 AT4G00150 24.485 437 258 18 18 443 178 553 4.07e-13 72.0

Find 96 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATAACGTAACCAATTT+TGG 0.129129 2.1:+12932952 None:intergenic
CCTGAACCAGAAGCACTTAA+AGG 0.212847 2.1:-12933686 MS.gene35774:CDS
ATCCTAGCATGTTGAATCTT+AGG 0.231235 2.1:-12933001 MS.gene35774:CDS
TGGTTATAAATTGCCAAAAT+TGG 0.234975 2.1:-12932965 MS.gene35774:CDS
AAAACAGTTTCCTCACAAAA+TGG 0.244613 2.1:+12932588 None:intergenic
CCTTTAAGTGCTTCTGGTTC+AGG 0.294156 2.1:+12933686 None:intergenic
TAGGCCTATGGTTCCTCCTT+TGG 0.297810 2.1:-12933192 MS.gene35774:CDS
CAGGAAGAGGAGGAGTGTTT+TGG 0.299955 2.1:+12933705 None:intergenic
CATCAAAGGGTATCCATAAA+TGG 0.302600 2.1:+12932781 None:intergenic
AGACACCCTTTAAGTGCTTC+TGG 0.308751 2.1:+12933680 None:intergenic
CTAAGTGAAGGAGGACCTTC+TGG 0.319718 2.1:+12933233 None:intergenic
GAAACCATGAAGTGAGTCTT+TGG 0.321815 2.1:+12933522 None:intergenic
GTCCTTTGACAACTATCTTT+AGG 0.332169 2.1:+12932741 None:intergenic
CTAACCAAAGACTCACTTCA+TGG 0.347970 2.1:-12933526 MS.gene35774:CDS
TAACCCTCAAATAGTGCTAT+TGG 0.350512 2.1:-12932865 MS.gene35774:CDS
TGATTTGAAAATTTCATTGT+TGG 0.362564 2.1:+12933346 None:intergenic
CTTGGGTCCCTAGTGAAATT+CGG 0.366646 2.1:-12932458 MS.gene35774:CDS
GGGTGTCTTGGAAGCTTAGA+TGG 0.372435 2.1:-12933665 MS.gene35774:CDS
ATCAACATACCCGGCGAGTT+CGG 0.379075 2.1:+12933403 None:intergenic
CCAATAACATTAAACTCAAA+AGG 0.380341 2.1:+12933107 None:intergenic
AACAACGTTGCTTCACCATT+AGG 0.392639 2.1:-12933554 MS.gene35774:CDS
TTAATTCCTTGGCATAGGTT+TGG 0.394976 2.1:-12933380 MS.gene35774:CDS
ACTCCATTGTGCAAGTGCTT+TGG 0.397900 2.1:-12933621 MS.gene35774:CDS
GAGAATATTATAAGCTTTGA+AGG 0.397984 2.1:-12932687 MS.gene35774:CDS
TGGTGTGTGTTGAAGAGTGT+TGG 0.399999 2.1:+12933725 None:intergenic
ATGGTGAAGCAACGTTGTTT+AGG 0.411496 2.1:+12933558 None:intergenic
AGGATAGAGAGATTGGAGTC+AGG 0.412485 2.1:-12932657 MS.gene35774:CDS
TCTTAGGGAAGATGAAGCTT+TGG 0.413076 2.1:-12932985 MS.gene35774:CDS
TTGGGTCCCTAGTGAAATTC+GGG 0.414052 2.1:-12932457 MS.gene35774:CDS
GCATCAAAGGATAGAGAGAT+TGG 0.414417 2.1:-12932664 MS.gene35774:CDS
CATGAAGTAGGTCAACGTTT+AGG 0.420677 2.1:-12933155 MS.gene35774:CDS
CTTTGGTTAGTGTCACCTAA+TGG 0.430482 2.1:+12933539 None:intergenic
GGTGTGTGTTGAAGAGTGTT+GGG 0.432610 2.1:+12933726 None:intergenic
AATCAATTAAGGGATTCAAA+AGG 0.434752 2.1:-12933328 MS.gene35774:CDS
CTGCGTGTTTGCGACCGCTT+GGG 0.440188 2.1:-12932475 MS.gene35774:CDS
ATGGATACCCTTTGATGCAC+TGG 0.441346 2.1:-12932775 MS.gene35774:CDS
CATTAATCTCTAGAGCTTCA+AGG 0.442427 2.1:-12933493 MS.gene35774:CDS
GCTGCGTGTTTGCGACCGCT+TGG 0.447845 2.1:-12932476 MS.gene35774:CDS
AGTGCTTCTGGTTCAGGAAG+AGG 0.451746 2.1:+12933692 None:intergenic
CTGAACCAGAAGCACTTAAA+GGG 0.452138 2.1:-12933685 MS.gene35774:CDS
TCCTAGCATGTTGAATCTTA+GGG 0.452667 2.1:-12933000 MS.gene35774:CDS
GGATAGAGAGATTGGAGTCA+GGG 0.452893 2.1:-12932656 MS.gene35774:CDS
TCAACCAATAGCACTATTTG+AGG 0.464072 2.1:+12932861 None:intergenic
GGTATGTTGATTTAATTCCT+TGG 0.469050 2.1:-12933391 MS.gene35774:CDS
TTATTTATCTGATGATAGAA+AGG 0.472508 2.1:-12932937 MS.gene35774:CDS
AAAGTAGGCCATTGCATACA+AGG 0.474102 2.1:+12933278 None:intergenic
ATCAATTAAGGGATTCAAAA+GGG 0.476229 2.1:-12933327 MS.gene35774:CDS
AAAAGTGTCCAGTGCATCAA+AGG 0.477866 2.1:+12932767 None:intergenic
CCTTAGCTCAACAAGTTATG+TGG 0.481567 2.1:-12933583 MS.gene35774:CDS
AGTGAAATTCGGGATCATAT+CGG 0.488833 2.1:-12932447 MS.gene35774:CDS
CCTTTGAAATTCATTGAAGT+AGG 0.489707 2.1:+12933464 None:intergenic
AAAGCATGTTGGTTCTTACA+TGG 0.498679 2.1:-12932509 MS.gene35774:CDS
GAAGCACTTAAAGGGTGTCT+TGG 0.503731 2.1:-12933677 MS.gene35774:CDS
CAATAACATTAAACTCAAAA+GGG 0.504289 2.1:+12933108 None:intergenic
TTGATTTAATTCCTTGGCAT+AGG 0.515662 2.1:-12933385 MS.gene35774:CDS
CCTACTTCAATGAATTTCAA+AGG 0.532194 2.1:-12933464 MS.gene35774:CDS
CCACATAACTTGTTGAGCTA+AGG 0.533265 2.1:+12933583 None:intergenic
TATTTATCTGATGATAGAAA+GGG 0.534012 2.1:-12932936 MS.gene35774:CDS
GCCAAAGAATCTATAAAAGT+AGG 0.539244 2.1:+12933263 None:intergenic
CAACCAATAGCACTATTTGA+GGG 0.543059 2.1:+12932862 None:intergenic
GAACCATAGGCCTAAAAGAT+GGG 0.544386 2.1:+12933201 None:intergenic
ATGTTGGTTCTTACATGGAA+AGG 0.544446 2.1:-12932504 MS.gene35774:CDS
TAAGCTTTGAAGGGCATCAA+AGG 0.546684 2.1:-12932677 MS.gene35774:CDS
GGAACCATAGGCCTAAAAGA+TGG 0.547678 2.1:+12933200 None:intergenic
ATGTCGGTTACCGAACTCGC+CGG 0.550372 2.1:-12933413 MS.gene35774:CDS
GATGAACATGCTAGTGGATG+GGG 0.551613 2.1:-12932546 MS.gene35774:CDS
ATATGATCCCGAATTTCACT+AGG 0.561630 2.1:+12932450 None:intergenic
AGAATATTATAAGCTTTGAA+GGG 0.562232 2.1:-12932686 MS.gene35774:CDS
AACATATCAATACATGAAGT+AGG 0.562253 2.1:-12933167 MS.gene35774:CDS
AGCTTTCATGTGAACGTGCA+TGG 0.563642 2.1:+12933784 None:intergenic
TGCCTAAAGATAGTTGTCAA+AGG 0.566343 2.1:-12932743 MS.gene35774:CDS
GTAACTACACAATTCAAAGA+AGG 0.569120 2.1:-12933439 MS.gene35774:CDS
TGTCGGTTACCGAACTCGCC+GGG 0.570400 2.1:-12933412 MS.gene35774:CDS
AATAGTGCTATTGGTTGATG+AGG 0.571491 2.1:-12932856 MS.gene35774:CDS
GCATAACACCTTGTATGCAA+TGG 0.571925 2.1:-12933286 MS.gene35774:CDS
AAAGTGTCCAGTGCATCAAA+GGG 0.575835 2.1:+12932768 None:intergenic
TCCCTAAGATTCAACATGCT+AGG 0.588496 2.1:+12932999 None:intergenic
TGTATTGATATGTTAACCAA+AGG 0.588615 2.1:+12933176 None:intergenic
TTAACCAAAGGAGGAACCAT+AGG 0.589947 2.1:+12933188 None:intergenic
ATTGATATGTTAACCAAAGG+AGG 0.601377 2.1:+12933179 None:intergenic
TTGATGAACATGCTAGTGGA+TGG 0.601862 2.1:-12932548 MS.gene35774:CDS
TGATGAACATGCTAGTGGAT+GGG 0.604800 2.1:-12932547 MS.gene35774:CDS
TCTTTGGCTAAATTACCAGA+AGG 0.605851 2.1:-12933248 MS.gene35774:CDS
TTGCTTGATGAACATGCTAG+TGG 0.606672 2.1:-12932552 MS.gene35774:CDS
GCTTCTGGTTCAGGAAGAGG+AGG 0.607512 2.1:+12933695 None:intergenic
CTTAGCTCAACAAGTTATGT+GGG 0.611843 2.1:-12933582 MS.gene35774:CDS
AGGAATTAAATCAACATACC+CGG 0.613372 2.1:+12933394 None:intergenic
GAAAAGAGAAGAAAGCATGT+TGG 0.614602 2.1:-12932520 MS.gene35774:CDS
ACAGAGTTTGAATCTGACAT+AGG 0.622989 2.1:-12932720 MS.gene35774:CDS
AATTCAAAGAAGGTTGATGT+CGG 0.634129 2.1:-12933429 MS.gene35774:CDS
GGGACTGTGATTCTAAGTGA+AGG 0.639863 2.1:+12933221 None:intergenic
TATGATCCCGAATTTCACTA+GGG 0.650286 2.1:+12932451 None:intergenic
ACTGTGATTCTAAGTGAAGG+AGG 0.660820 2.1:+12933224 None:intergenic
AATTCGGGATCATATCGGCA+TGG 0.665545 2.1:-12932442 MS.gene35774:CDS
TTTCCAAAGCACTTGCACAA+TGG 0.673097 2.1:+12933618 None:intergenic
AATTTCACTAGGGACCCAAG+CGG 0.769651 2.1:+12932461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTTTCAAATCAATTAA+GGG - chr2.1:12932869-12932888 MS.gene35774:CDS 15.0%
!!! TGAAATTTTCAAATCAATTA+AGG - chr2.1:12932868-12932887 MS.gene35774:CDS 15.0%
!! CAATAACATTAAACTCAAAA+GGG + chr2.1:12933102-12933121 None:intergenic 20.0%
!! TATTTATCTGATGATAGAAA+GGG - chr2.1:12933271-12933290 MS.gene35774:CDS 20.0%
!! TGATTTGAAAATTTCATTGT+TGG + chr2.1:12932864-12932883 None:intergenic 20.0%
!! TTATTTATCTGATGATAGAA+AGG - chr2.1:12933270-12933289 MS.gene35774:CDS 20.0%
!!! AGAATATTATAAGCTTTGAA+GGG - chr2.1:12933521-12933540 MS.gene35774:CDS 20.0%
!!! AGGTAATTTTGCAAAATTTA+AGG - chr2.1:12933072-12933091 MS.gene35774:CDS 20.0%
!!! ATAAATAACGTAACCAATTT+TGG + chr2.1:12933258-12933277 None:intergenic 20.0%
!!! GCTTTTATGAATTTAGTTAA+AGG - chr2.1:12933316-12933335 MS.gene35774:CDS 20.0%
! AACATATCAATACATGAAGT+AGG - chr2.1:12933040-12933059 MS.gene35774:CDS 25.0%
! AATCAATTAAGGGATTCAAA+AGG - chr2.1:12932879-12932898 MS.gene35774:CDS 25.0%
! ATCAATTAAGGGATTCAAAA+GGG - chr2.1:12932880-12932899 MS.gene35774:CDS 25.0%
! CCAATAACATTAAACTCAAA+AGG + chr2.1:12933103-12933122 None:intergenic 25.0%
! TGGTTATAAATTGCCAAAAT+TGG - chr2.1:12933242-12933261 MS.gene35774:CDS 25.0%
!! GAGAATATTATAAGCTTTGA+AGG - chr2.1:12933520-12933539 MS.gene35774:CDS 25.0%
!! TGTATTGATATGTTAACCAA+AGG + chr2.1:12933034-12933053 None:intergenic 25.0%
!!! CCTTTTGAGTTTAATGTTAT+TGG - chr2.1:12933100-12933119 MS.gene35774:CDS 25.0%
AAAACAGTTTCCTCACAAAA+TGG + chr2.1:12933622-12933641 None:intergenic 30.0%
AGGAATTAAATCAACATACC+CGG + chr2.1:12932816-12932835 None:intergenic 30.0%
ATTGATATGTTAACCAAAGG+AGG + chr2.1:12933031-12933050 None:intergenic 30.0%
CCTACTTCAATGAATTTCAA+AGG - chr2.1:12932743-12932762 MS.gene35774:CDS 30.0%
GCCAAAGAATCTATAAAAGT+AGG + chr2.1:12932947-12932966 None:intergenic 30.0%
GTAACTACACAATTCAAAGA+AGG - chr2.1:12932768-12932787 MS.gene35774:CDS 30.0%
TCACAAAGAATGAAAAATGC+TGG - chr2.1:12933583-12933602 MS.gene35774:CDS 30.0%
TTGATTTAATTCCTTGGCAT+AGG - chr2.1:12932822-12932841 MS.gene35774:CDS 30.0%
! ATTTTGTGAGGAAACTGTTT+TGG - chr2.1:12933621-12933640 MS.gene35774:CDS 30.0%
! CTGCTTCTTTTAACCATTTA+TGG - chr2.1:12933413-12933432 MS.gene35774:CDS 30.0%
!! AATTCAAAGAAGGTTGATGT+CGG - chr2.1:12932778-12932797 MS.gene35774:CDS 30.0%
!! CCTTTGAAATTCATTGAAGT+AGG + chr2.1:12932746-12932765 None:intergenic 30.0%
!! GCCTACTTTTATAGATTCTT+TGG - chr2.1:12932943-12932962 MS.gene35774:CDS 30.0%
!! GGTATGTTGATTTAATTCCT+TGG - chr2.1:12932816-12932835 MS.gene35774:CDS 30.0%
ACAGAGTTTGAATCTGACAT+AGG - chr2.1:12933487-12933506 MS.gene35774:CDS 35.0%
AGTGAAATTCGGGATCATAT+CGG - chr2.1:12933760-12933779 MS.gene35774:CDS 35.0%
ATATGATCCCGAATTTCACT+AGG + chr2.1:12933760-12933779 None:intergenic 35.0%
ATCCTAGCATGTTGAATCTT+AGG - chr2.1:12933206-12933225 MS.gene35774:CDS 35.0%
CAACCAATAGCACTATTTGA+GGG + chr2.1:12933348-12933367 None:intergenic 35.0%
CTTAGCTCAACAAGTTATGT+GGG - chr2.1:12932625-12932644 MS.gene35774:CDS 35.0%
GTCCTTTGACAACTATCTTT+AGG + chr2.1:12933469-12933488 None:intergenic 35.0%
TAACCCTCAAATAGTGCTAT+TGG - chr2.1:12933342-12933361 MS.gene35774:CDS 35.0%
TATGATCCCGAATTTCACTA+GGG + chr2.1:12933759-12933778 None:intergenic 35.0%
TCAACCAATAGCACTATTTG+AGG + chr2.1:12933349-12933368 None:intergenic 35.0%
TCCTAGCATGTTGAATCTTA+GGG - chr2.1:12933207-12933226 MS.gene35774:CDS 35.0%
TCTTTGGCTAAATTACCAGA+AGG - chr2.1:12932959-12932978 MS.gene35774:CDS 35.0%
TGCCTAAAGATAGTTGTCAA+AGG - chr2.1:12933464-12933483 MS.gene35774:CDS 35.0%
TTAATTCCTTGGCATAGGTT+TGG - chr2.1:12932827-12932846 MS.gene35774:CDS 35.0%
! AATAGTGCTATTGGTTGATG+AGG - chr2.1:12933351-12933370 MS.gene35774:CDS 35.0%
! CTTTAGTGTTCCATTTTGTG+AGG - chr2.1:12933609-12933628 MS.gene35774:CDS 35.0%
! GAAAAGAGAAGAAAGCATGT+TGG - chr2.1:12933687-12933706 MS.gene35774:CDS 35.0%
!! AAAGCATGTTGGTTCTTACA+TGG - chr2.1:12933698-12933717 MS.gene35774:CDS 35.0%
!! ATGTTGGTTCTTACATGGAA+AGG - chr2.1:12933703-12933722 MS.gene35774:CDS 35.0%
!! CATCAAAGGGTATCCATAAA+TGG + chr2.1:12933429-12933448 None:intergenic 35.0%
!! CATTAATCTCTAGAGCTTCA+AGG - chr2.1:12932714-12932733 MS.gene35774:CDS 35.0%
!!! GAAACTGTTTTGGAAGTCAA+GGG - chr2.1:12933631-12933650 MS.gene35774:CDS 35.0%
!!! TGTTGGGTTTTGAAAGGAAA+TGG + chr2.1:12932468-12932487 None:intergenic 35.0%
AAAGTAGGCCATTGCATACA+AGG + chr2.1:12932932-12932951 None:intergenic 40.0%
AACAACGTTGCTTCACCATT+AGG - chr2.1:12932653-12932672 MS.gene35774:CDS 40.0%
ACTGTGATTCTAAGTGAAGG+AGG + chr2.1:12932986-12933005 None:intergenic 40.0%
ATGGTGAAGCAACGTTGTTT+AGG + chr2.1:12932652-12932671 None:intergenic 40.0%
CAAAAACCAAACCTATGCCA+AGG + chr2.1:12932836-12932855 None:intergenic 40.0%
CATGAAGTAGGTCAACGTTT+AGG - chr2.1:12933052-12933071 MS.gene35774:CDS 40.0%
CCTTAGCTCAACAAGTTATG+TGG - chr2.1:12932624-12932643 MS.gene35774:CDS 40.0%
CTAACCAAAGACTCACTTCA+TGG - chr2.1:12932681-12932700 MS.gene35774:CDS 40.0%
CTTTGGTTAGTGTCACCTAA+TGG + chr2.1:12932671-12932690 None:intergenic 40.0%
GAAACCATGAAGTGAGTCTT+TGG + chr2.1:12932688-12932707 None:intergenic 40.0%
GAACCATAGGCCTAAAAGAT+GGG + chr2.1:12933009-12933028 None:intergenic 40.0%
GCATAACACCTTGTATGCAA+TGG - chr2.1:12932921-12932940 MS.gene35774:CDS 40.0%
GCATCAAAGGATAGAGAGAT+TGG - chr2.1:12933543-12933562 MS.gene35774:CDS 40.0%
TCCCTAAGATTCAACATGCT+AGG + chr2.1:12933211-12933230 None:intergenic 40.0%
TCTTAGGGAAGATGAAGCTT+TGG - chr2.1:12933222-12933241 MS.gene35774:CDS 40.0%
TTAACCAAAGGAGGAACCAT+AGG + chr2.1:12933022-12933041 None:intergenic 40.0%
TTGATGAACATGCTAGTGGA+TGG - chr2.1:12933659-12933678 MS.gene35774:CDS 40.0%
TTGCTTGATGAACATGCTAG+TGG - chr2.1:12933655-12933674 MS.gene35774:CDS 40.0%
! AAAGTGTCCAGTGCATCAAA+GGG + chr2.1:12933442-12933461 None:intergenic 40.0%
! GAATCACAGTCCCATCTTTT+AGG - chr2.1:12932996-12933015 MS.gene35774:CDS 40.0%
! TGATGAACATGCTAGTGGAT+GGG - chr2.1:12933660-12933679 MS.gene35774:CDS 40.0%
! TTTCCAAAGCACTTGCACAA+TGG + chr2.1:12932592-12932611 None:intergenic 40.0%
!! AAAAGTGTCCAGTGCATCAA+AGG + chr2.1:12933443-12933462 None:intergenic 40.0%
!! CCACATAACTTGTTGAGCTA+AGG + chr2.1:12932627-12932646 None:intergenic 40.0%
!! CTGAACCAGAAGCACTTAAA+GGG - chr2.1:12932522-12932541 MS.gene35774:CDS 40.0%
!! TAAGCTTTGAAGGGCATCAA+AGG - chr2.1:12933530-12933549 MS.gene35774:CDS 40.0%
!!! GAAGAGTGTTGGGTTTTGAA+AGG + chr2.1:12932474-12932493 None:intergenic 40.0%
!!! GGAAACTGTTTTGGAAGTCA+AGG - chr2.1:12933630-12933649 MS.gene35774:CDS 40.0%
AATTCGGGATCATATCGGCA+TGG - chr2.1:12933765-12933784 MS.gene35774:CDS 45.0%
AATTTCACTAGGGACCCAAG+CGG + chr2.1:12933749-12933768 None:intergenic 45.0%
ACTCCATTGTGCAAGTGCTT+TGG - chr2.1:12932586-12932605 MS.gene35774:CDS 45.0%
AGACACCCTTTAAGTGCTTC+TGG + chr2.1:12932530-12932549 None:intergenic 45.0%
AGGATAGAGAGATTGGAGTC+AGG - chr2.1:12933550-12933569 MS.gene35774:CDS 45.0%
ATGGATACCCTTTGATGCAC+TGG - chr2.1:12933432-12933451 MS.gene35774:CDS 45.0%
CTTGGGTCCCTAGTGAAATT+CGG - chr2.1:12933749-12933768 MS.gene35774:CDS 45.0%
GGAACCATAGGCCTAAAAGA+TGG + chr2.1:12933010-12933029 None:intergenic 45.0%
GGATAGAGAGATTGGAGTCA+GGG - chr2.1:12933551-12933570 MS.gene35774:CDS 45.0%
GGGACTGTGATTCTAAGTGA+AGG + chr2.1:12932989-12933008 None:intergenic 45.0%
TTGGGTCCCTAGTGAAATTC+GGG - chr2.1:12933750-12933769 MS.gene35774:CDS 45.0%
! AGTCCCATCTTTTAGGCCTA+TGG - chr2.1:12933003-12933022 MS.gene35774:CDS 45.0%
! GATGAACATGCTAGTGGATG+GGG - chr2.1:12933661-12933680 MS.gene35774:CDS 45.0%
! TGGTGTGTGTTGAAGAGTGT+TGG + chr2.1:12932485-12932504 None:intergenic 45.0%
!! CCTGAACCAGAAGCACTTAA+AGG - chr2.1:12932521-12932540 MS.gene35774:CDS 45.0%
!! CCTTTAAGTGCTTCTGGTTC+AGG + chr2.1:12932524-12932543 None:intergenic 45.0%
!! GAAGCACTTAAAGGGTGTCT+TGG - chr2.1:12932530-12932549 MS.gene35774:CDS 45.0%
!! GGTGTGTGTTGAAGAGTGTT+GGG + chr2.1:12932484-12932503 None:intergenic 45.0%
ATCAACATACCCGGCGAGTT+CGG + chr2.1:12932807-12932826 None:intergenic 50.0%
CTAAGTGAAGGAGGACCTTC+TGG + chr2.1:12932977-12932996 None:intergenic 50.0%
GGGTGTCTTGGAAGCTTAGA+TGG - chr2.1:12932542-12932561 MS.gene35774:CDS 50.0%
TAGGCCTATGGTTCCTCCTT+TGG - chr2.1:12933015-12933034 MS.gene35774:CDS 50.0%
!! AGTGCTTCTGGTTCAGGAAG+AGG + chr2.1:12932518-12932537 None:intergenic 50.0%
!!! CAGGAAGAGGAGGAGTGTTT+TGG + chr2.1:12932505-12932524 None:intergenic 50.0%
! ATGTCGGTTACCGAACTCGC+CGG - chr2.1:12932794-12932813 MS.gene35774:CDS 55.0%
!! GCTTCTGGTTCAGGAAGAGG+AGG + chr2.1:12932515-12932534 None:intergenic 55.0%
CTGCGTGTTTGCGACCGCTT+GGG - chr2.1:12933732-12933751 MS.gene35774:CDS 60.0%
! TGTCGGTTACCGAACTCGCC+GGG - chr2.1:12932795-12932814 MS.gene35774:CDS 60.0%
GCTGCGTGTTTGCGACCGCT+TGG - chr2.1:12933731-12933750 MS.gene35774:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 12932425 12933804 12932425 ID=MS.gene35774
chr2.1 mRNA 12932425 12933804 12932425 ID=MS.gene35774.t1;Parent=MS.gene35774
chr2.1 exon 12932425 12933804 12932425 ID=MS.gene35774.t1.exon1;Parent=MS.gene35774.t1
chr2.1 CDS 12932425 12933804 12932425 ID=cds.MS.gene35774.t1;Parent=MS.gene35774.t1
Gene Sequence

>MS.gene35774

ATGCACGTTCACATGAAAGCTGAGTTAAAAGCAGCAACATCCATTTCCTTTCAAAACCCAACACTCTTCAACACACACCAAAACACTCCTCCTCTTCCTGAACCAGAAGCACTTAAAGGGTGTCTTGGAAGCTTAGATGGAGCATGTATTGAAAAGCTCTTACTCCATTGTGCAAGTGCTTTGGAAAGCAATGATATAACCTTAGCTCAACAAGTTATGTGGGTCCTAAACAACGTTGCTTCACCATTAGGTGACACTAACCAAAGACTCACTTCATGGTTTCTAAGAGCATTAATCTCTAGAGCTTCAAGGATTTGTCCTACTTCAATGAATTTCAAAGGAAGTAACTACACAATTCAAAGAAGGTTGATGTCGGTTACCGAACTCGCCGGGTATGTTGATTTAATTCCTTGGCATAGGTTTGGTTTTTGTGCTTCCAACAATGAAATTTTCAAATCAATTAAGGGATTCAAAAGGGTACATATTTTAGATTTTAGCATAACACCTTGTATGCAATGGCCTACTTTTATAGATTCTTTGGCTAAATTACCAGAAGGTCCTCCTTCACTTAGAATCACAGTCCCATCTTTTAGGCCTATGGTTCCTCCTTTGGTTAACATATCAATACATGAAGTAGGTCAACGTTTAGGTAATTTTGCAAAATTTAAGGATGTCCCTTTTGAGTTTAATGTTATTGGAGATAATGTTTCATTGACTTCTGAAGATTTGAGCAATATTGAATCAACAAATTTTCATTTTGAATCAATGTTGAGTCTCTTGAATCCTAGCATGTTGAATCTTAGGGAAGATGAAGCTTTGGTTATAAATTGCCAAAATTGGTTACGTTATTTATCTGATGATAGAAAGGGTCAAAATATTTCACTTAGAGATGCTTTTATGAATTTAGTTAAAGGACTTAACCCTCAAATAGTGCTATTGGTTGATGAGGATTGTGATCTTAGTGCATCAAGTTTAACATCAAGAATTACTGCTTCTTTTAACCATTTATGGATACCCTTTGATGCACTGGACACTTTTTTGCCTAAAGATAGTTGTCAAAGGACAGAGTTTGAATCTGACATAGGACAAAAAATTGAGAATATTATAAGCTTTGAAGGGCATCAAAGGATAGAGAGATTGGAGTCAGGGATGCAAATGTCACAAAGAATGAAAAATGCTGGTTACTTTAGTGTTCCATTTTGTGAGGAAACTGTTTTGGAAGTCAAGGGTTTGCTTGATGAACATGCTAGTGGATGGGGAATGAAAAGAGAAGAAAGCATGTTGGTTCTTACATGGAAAGGAAATAGCTGCGTGTTTGCGACCGCTTGGGTCCCTAGTGAAATTCGGGATCATATCGGCATGGATGCACGTGTGTCGTAG
Protein sequence

>MS.gene35774.t1

MHVHMKAELKAATSISFQNPTLFNTHQNTPPLPEPEALKGCLGSLDGACIEKLLLHCASALESNDITLAQQVMWVLNNVASPLGDTNQRLTSWFLRALISRASRICPTSMNFKGSNYTIQRRLMSVTELAGYVDLIPWHRFGFCASNNEIFKSIKGFKRVHILDFSITPCMQWPTFIDSLAKLPEGPPSLRITVPSFRPMVPPLVNISIHEVGQRLGNFAKFKDVPFEFNVIGDNVSLTSEDLSNIESTNFHFESMLSLLNPSMLNLREDEALVINCQNWLRYLSDDRKGQNISLRDAFMNLVKGLNPQIVLLVDEDCDLSASSLTSRITASFNHLWIPFDALDTFLPKDSCQRTEFESDIGQKIENIISFEGHQRIERLESGMQMSQRMKNAGYFSVPFCEETVLEVKGLLDEHASGWGMKREESMLVLTWKGNSCVFATAWVPSEIRDHIGMDARVS