AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35994

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35994.t1 MTR_6g090505 57.820 422 76 5 1 380 1 362 6.87e-158 454
MS.gene35994.t1 MTR_0558s0020 88.312 231 27 0 1 231 66 296 1.69e-152 438
MS.gene35994.t1 MTR_1g083640 89.706 204 21 0 1 204 81 284 1.52e-135 395
MS.gene35994.t1 MTR_8g446020 86.829 205 27 0 27 231 52 256 3.31e-131 382
MS.gene35994.t1 MTR_2g014100 86.047 172 24 0 1 172 74 245 1.37e-105 317
MS.gene35994.t1 MTR_0337s0040 77.720 193 43 0 4 196 30 222 5.80e-105 314
MS.gene35994.t1 MTR_3g028680 81.595 163 27 1 383 542 1 163 8.04e-91 276
MS.gene35994.t1 MTR_1g055240 88.028 142 17 0 27 168 3 144 1.07e-86 265
MS.gene35994.t1 MTR_0019s0290 82.237 152 27 0 18 169 9 160 9.11e-86 263
MS.gene35994.t1 MTR_2g067090 80.000 160 29 1 386 542 99 258 1.23e-82 259
MS.gene35994.t1 MTR_3g008240 85.507 138 20 0 1 138 66 203 1.26e-79 248
MS.gene35994.t1 MTR_4g040430 84.058 138 18 1 1 138 65 198 4.46e-77 241
MS.gene35994.t1 MTR_4g133495 62.766 188 43 3 341 525 88 251 9.67e-69 223
MS.gene35994.t1 MTR_4g133340 62.766 188 43 3 341 525 88 251 9.67e-69 223
MS.gene35994.t1 MTR_5g071590 78.846 104 22 0 1 104 78 181 5.26e-55 184
MS.gene35994.t1 MTR_0337s0060 52.809 178 25 4 350 524 118 239 6.40e-43 153
MS.gene35994.t1 MTR_6g051425 32.601 273 156 5 21 277 64 324 4.29e-36 137
MS.gene35994.t1 MTR_1281s0010 77.778 63 14 0 1 63 47 109 3.14e-27 106
MS.gene35994.t1 MTR_2g067130 65.116 86 24 2 460 542 46 128 1.90e-25 102
MS.gene35994.t1 MTR_1g070355 24.444 270 189 3 21 289 119 374 6.13e-15 78.2
MS.gene35994.t1 MTR_0337s0050 75.000 36 9 0 195 230 14 49 3.43e-14 68.2
MS.gene35994.t1 MTR_0402s0040 26.667 150 95 3 20 169 71 205 6.94e-13 71.2
MS.gene35994.t1 MTR_4g035820 25.941 239 155 8 16 235 89 324 4.11e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35994.t1 AT4G38180 28.533 368 208 8 20 346 118 471 1.39e-41 160
MS.gene35994.t1 AT3G06250 27.419 248 142 3 135 344 295 542 2.32e-28 120
MS.gene35994.t1 AT3G06250 27.419 248 142 3 135 344 295 542 2.32e-28 120
MS.gene35994.t1 AT3G06250 27.419 248 142 3 135 344 295 542 2.32e-28 120
MS.gene35994.t1 AT5G28530 28.470 281 176 5 21 289 100 367 1.67e-27 117
MS.gene35994.t1 AT5G28530 28.470 281 176 5 21 289 100 367 2.02e-27 117
MS.gene35994.t1 AT5G28530 28.470 281 176 5 21 289 100 367 2.02e-27 117
MS.gene35994.t1 AT2G27110 27.393 303 164 6 86 346 130 418 2.82e-27 117
MS.gene35994.t1 AT2G27110 27.393 303 164 6 86 346 130 418 2.82e-27 117
MS.gene35994.t1 AT4G15090 26.126 333 195 6 59 346 121 447 1.06e-26 115
MS.gene35994.t1 AT4G15090 31.126 151 87 3 356 506 545 678 5.40e-14 75.5
MS.gene35994.t1 AT4G15090 26.126 333 195 6 59 346 121 447 1.06e-26 115
MS.gene35994.t1 AT4G15090 31.126 151 87 3 356 506 545 678 5.40e-14 75.5
MS.gene35994.t1 AT4G15090 26.126 333 195 6 59 346 121 447 1.06e-26 115
MS.gene35994.t1 AT4G15090 31.126 151 87 3 356 506 545 678 5.40e-14 75.5
MS.gene35994.t1 AT4G15090 26.126 333 195 6 59 346 121 447 1.06e-26 115
MS.gene35994.t1 AT4G15090 31.126 151 87 3 356 506 545 678 5.40e-14 75.5
MS.gene35994.t1 AT4G15090 26.126 333 195 6 59 346 121 447 1.06e-26 115
MS.gene35994.t1 AT4G15090 31.126 151 87 3 356 506 545 678 5.40e-14 75.5
MS.gene35994.t1 AT2G27110 28.571 238 130 3 149 346 36 273 2.25e-26 114
MS.gene35994.t1 AT1G10240 24.576 354 209 10 43 345 102 448 2.30e-24 107
MS.gene35994.t1 AT1G10240 24.576 354 209 10 43 345 102 448 2.30e-24 107
MS.gene35994.t1 AT1G76320 27.046 281 173 7 5 281 27 279 1.02e-23 105
MS.gene35994.t1 AT5G18960 25.758 264 151 6 134 357 316 574 1.59e-23 105
MS.gene35994.t1 AT5G18960 25.758 264 151 6 134 357 316 574 1.59e-23 105
MS.gene35994.t1 AT1G76320 27.046 281 173 7 5 281 27 279 1.63e-23 105
MS.gene35994.t1 AT1G76320 27.046 281 173 7 5 281 27 279 1.66e-23 105
MS.gene35994.t1 AT1G76320 27.046 281 173 7 5 281 27 279 1.66e-23 105
MS.gene35994.t1 AT1G80010 34.899 149 95 2 146 293 253 400 9.50e-23 102
MS.gene35994.t1 AT4G38170 26.697 221 109 4 171 346 24 236 1.05e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 31 259 1.48e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.49e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.49e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.49e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.49e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 31 259 1.49e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.50e-22 102
MS.gene35994.t1 AT2G32250 27.336 289 148 6 9 295 72 300 1.50e-22 102
MS.gene35994.t1 AT3G22170 20.833 384 233 7 6 346 101 456 1.55e-19 93.2
MS.gene35994.t1 AT3G22170 20.833 384 233 7 6 346 101 456 1.55e-19 93.2
MS.gene35994.t1 AT1G52520 32.061 131 88 1 163 293 246 375 1.70e-17 86.7
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 163 315 3.45e-16 82.4
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 495 615 1.72e-11 67.4
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 163 315 3.45e-16 82.4
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 495 615 1.72e-11 67.4
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 106 258 3.92e-16 82.0
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 438 558 1.49e-11 67.8
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 177 329 4.00e-16 82.4
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 509 629 1.76e-11 67.4
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 163 315 4.02e-16 82.0
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 495 615 1.71e-11 67.4
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 163 315 4.24e-16 82.0
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 495 615 1.74e-11 67.4
MS.gene35994.t1 AT4G19990 32.026 153 100 2 155 303 163 315 4.24e-16 82.0
MS.gene35994.t1 AT4G19990 34.586 133 73 3 356 486 495 615 1.74e-11 67.4

Find 80 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCGCTTCTTTGACCTAAT+TGG 0.186128 6.2:-24232425 None:intergenic
TACATTTCAGCTGCCTTTCT+TGG 0.197151 6.2:+24231009 MS.gene35994:intron
CAACAAGATTATGCTCTATT+TGG 0.198611 6.2:+24230496 MS.gene35994:CDS
CATCGTAAGAGTGTGTTATT+TGG 0.276792 6.2:+24231042 MS.gene35994:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 6.2:-24231136 None:intergenic
TTCTTTGACCTAATTGGATT+TGG 0.299426 6.2:-24232419 None:intergenic
AATCAGATTTCGACTACAAT+TGG 0.317950 6.2:+24231331 MS.gene35994:intron
TGTTCTTGAGGCAGGGAAAA+AGG 0.322946 6.2:+24232271 MS.gene35994:CDS
ACCAGCCTGGTCTGTGTATA+TGG 0.341000 6.2:-24231154 None:intergenic
GTCTTTAGTGCCAACATTCA+TGG 0.367086 6.2:-24232147 None:intergenic
TATGCATTGGTCTTCAGTTT+GGG 0.370760 6.2:+24231431 MS.gene35994:CDS
CTCAACGGTGTTCTTGAGGC+AGG 0.386173 6.2:+24232263 MS.gene35994:CDS
AACAATAAATATCGGCCATT+AGG 0.388497 6.2:+24230553 MS.gene35994:CDS
GTATGCATTGGTCTTCAGTT+TGG 0.392120 6.2:+24231430 MS.gene35994:CDS
TTCTCATACTCAACAAGAAC+TGG 0.395633 6.2:-24230016 None:intergenic
AAGAAAGACCAAATCCAATT+AGG 0.397771 6.2:+24232411 MS.gene35994:CDS
TTTGGTCTTTCTTTGAACTT+TGG 0.398856 6.2:-24232401 None:intergenic
GAGGAGTTTCTTATTTCTAA+AGG 0.403538 6.2:+24232296 MS.gene35994:CDS
CACGTCGTATGTTTGAGAAC+CGG 0.404458 6.2:+24231979 MS.gene35994:CDS
GATTATGATCTTCAAACCTT+GGG 0.413114 6.2:-24231902 None:intergenic
ATCGAACAAACAATAAATAT+CGG 0.414315 6.2:+24230545 MS.gene35994:CDS
GGGTTGTCACAGTCGACATT+AGG 0.419786 6.2:+24231937 MS.gene35994:CDS
CGATTATGATCTTCAAACCT+TGG 0.425681 6.2:-24231903 None:intergenic
GACCTACTTCAGACTTTAAG+TGG 0.427687 6.2:+24231385 MS.gene35994:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 6.2:+24230193 MS.gene35994:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 6.2:+24230075 MS.gene35994:CDS
CGCAAACACGTGTAGAAAGC+TGG 0.439904 6.2:-24232214 None:intergenic
TGGTCTGTGTATATGGTTTG+AGG 0.447350 6.2:-24231147 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 6.2:+24230058 MS.gene35994:CDS
CGCTTTAAAACATATTGTGC+AGG 0.447633 6.2:-24232080 None:intergenic
CGTTATGCTTGTAACAAAGA+AGG 0.449439 6.2:+24229941 MS.gene35994:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 6.2:+24230223 MS.gene35994:CDS
ATCAGATTTCGACTACAATT+GGG 0.464503 6.2:+24231332 MS.gene35994:intron
CTCAAGTAACGCAAATGCTC+TGG 0.465560 6.2:-24230163 None:intergenic
TGAATGTCTATAAGATATAT+AGG 0.472794 6.2:+24230107 MS.gene35994:CDS
ACGTCGTATGTTTGAGAACC+GGG 0.475165 6.2:+24231980 MS.gene35994:CDS
GAAGTGATGGGTCAAGGAGT+TGG 0.484292 6.2:+24230268 MS.gene35994:CDS
TCTAATGACGACTCGTCAAA+AGG 0.488508 6.2:+24230336 MS.gene35994:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 6.2:-24231022 None:intergenic
CGTCAAAAGGAGATGGTGGC+TGG 0.492100 6.2:+24230349 MS.gene35994:CDS
GAGAACATCTTATGAAGTGA+TGG 0.499875 6.2:+24230255 MS.gene35994:CDS
TGTCTTCTGCAGCATCAACT+TGG 0.502977 6.2:-24230441 None:intergenic
CTCATTCAAATCGTATGCAT+TGG 0.507227 6.2:+24231418 MS.gene35994:CDS
ATCATAATCGACTTGATGAA+AGG 0.507955 6.2:+24231916 MS.gene35994:CDS
TTTAAGTCCGAAAATCGAAA+AGG 0.508080 6.2:-24230307 None:intergenic
ATTGGTCTTCAGTTTGGGTG+AGG 0.509410 6.2:+24231436 MS.gene35994:CDS
GATCTTCAAACCTTGGGGAA+AGG 0.519004 6.2:-24231896 None:intergenic
TGTCTATAAGATATATAGGT+GGG 0.523740 6.2:+24230111 MS.gene35994:CDS
TCATAATCGACTTGATGAAA+GGG 0.526860 6.2:+24231917 MS.gene35994:CDS
ACAAAATAAGAAATCAGATA+AGG 0.530342 6.2:+24229907 MS.gene35994:CDS
CTGAAATCTACCATGAATGT+TGG 0.534154 6.2:+24232137 MS.gene35994:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 6.2:+24229952 MS.gene35994:CDS
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 6.2:+24229953 MS.gene35994:CDS
AAATGCTCTGGTTTATGTAA+TGG 0.557138 6.2:-24230151 None:intergenic
ACTTCAACAACGCACCAGCC+TGG 0.557139 6.2:-24231167 None:intergenic
GTGATGGGTCAAGGAGTTGG+AGG 0.568802 6.2:+24230271 MS.gene35994:CDS
TACACGTGTTTGCGCAGATG+CGG 0.571672 6.2:+24232223 MS.gene35994:CDS
TCTTATGAAGTGATGGGTCA+AGG 0.589029 6.2:+24230262 MS.gene35994:CDS
TCAACGGTGTTCTTGAGGCA+GGG 0.590097 6.2:+24232264 MS.gene35994:CDS
ACCATATACACAGACCAGGC+TGG 0.590176 6.2:+24231153 MS.gene35994:CDS
ATTTCTCAACGGTGTTCTTG+AGG 0.591378 6.2:+24232259 MS.gene35994:CDS
GCTGTCTGTGAACCATCTGT+TGG 0.593238 6.2:+24232371 MS.gene35994:CDS
TGAAGACTTAGAAGGCGTAA+CGG 0.598405 6.2:-24232339 None:intergenic
TTGAAGGAAACCTTTCCCCA+AGG 0.600566 6.2:+24231886 MS.gene35994:CDS
TTGATATCGTTGTGCTTGAG+TGG 0.601464 6.2:-24232171 None:intergenic
AGAACATCTTATGAAGTGAT+GGG 0.601988 6.2:+24230256 MS.gene35994:CDS
GACGACTCGTCAAAAGGAGA+TGG 0.604288 6.2:+24230342 MS.gene35994:CDS
ACGTGTTTGCGCAGATGCGG+AGG 0.605934 6.2:+24232226 MS.gene35994:CDS
GACTCGTCAAAAGGAGATGG+TGG 0.611157 6.2:+24230345 MS.gene35994:CDS
AGCCACTTAAAGTCTGAAGT+AGG 0.614939 6.2:-24231387 None:intergenic
AGAACTGGACTGTCACATGT+TGG 0.620791 6.2:-24230001 None:intergenic
GGGTCAAGGAGTTGGAGGCA+CGG 0.621882 6.2:+24230276 MS.gene35994:CDS
ATTATGATCTTCAAACCTTG+GGG 0.630358 6.2:-24231901 None:intergenic
TTTGGTGCAGTACCAACAGA+TGG 0.635771 6.2:-24232383 None:intergenic
ATGTCTATAAGATATATAGG+TGG 0.643489 6.2:+24230110 MS.gene35994:CDS
GATTTCAGCTTATCGACAGA+CGG 0.644926 6.2:-24232122 None:intergenic
GCATCTGAAATATTTCTCAA+CGG 0.661104 6.2:+24232248 MS.gene35994:CDS
CATGTCGACATAAGAAACCC+CGG 0.663279 6.2:-24231998 None:intergenic
CGTCGTATGTTTGAGAACCG+GGG 0.667385 6.2:+24231981 MS.gene35994:CDS
TCAAACCATATACACAGACC+AGG 0.671057 6.2:+24231149 MS.gene35994:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACGTATTTATTATTATATTT+TGG + chr6.2:24232722-24232741 MS.gene35994:intron 10.0%
!!! TGTTTTTTATGTTATAAGTT+AGG + chr6.2:24232551-24232570 MS.gene35994:intron 15.0%
!! AAACCAAATTAACAATCTAA+TGG - chr6.2:24232755-24232774 None:intergenic 20.0%
!! AAACCATAATTATGGTTTAA+TGG - chr6.2:24232798-24232817 None:intergenic 20.0%
!! AAACCATTAAACCATAATTA+TGG + chr6.2:24232792-24232811 MS.gene35994:intron 20.0%
!! AAACCATTAGATTGTTAATT+TGG + chr6.2:24232749-24232768 MS.gene35994:intron 20.0%
!! AAAGTGATTATGAAAAAGTT+AGG + chr6.2:24232450-24232469 MS.gene35994:intron 20.0%
!! AAGTGATTATGAAAAAGTTA+GGG + chr6.2:24232451-24232470 MS.gene35994:intron 20.0%
!! AATTCTTGATTGTTATGTAT+TGG + chr6.2:24232649-24232668 MS.gene35994:intron 20.0%
!! ACAAAATAAGAAATCAGATA+AGG + chr6.2:24229907-24229926 MS.gene35994:CDS 20.0%
!! ATCGAACAAACAATAAATAT+CGG + chr6.2:24230545-24230564 MS.gene35994:CDS 20.0%
!! TCTTAACTAAACCATAATTA+TGG - chr6.2:24232806-24232825 None:intergenic 20.0%
!! TGAATGTCTATAAGATATAT+AGG + chr6.2:24230107-24230126 MS.gene35994:CDS 20.0%
!!! AATTTAATGTTCTGGTTTAT+TGG + chr6.2:24230727-24230746 MS.gene35994:intron 20.0%
!!! ATGATGAAAAACTGTTTTAA+TGG + chr6.2:24231360-24231379 MS.gene35994:CDS 20.0%
!!! TGAATGTTTATATTGATTGA+AGG + chr6.2:24230699-24230718 MS.gene35994:intron 20.0%
! AAAACACCTTGAAAAACATT+CGG - chr6.2:24231198-24231217 None:intergenic 25.0%
! AGTACAAAATACTAACCTAA+TGG - chr6.2:24230571-24230590 None:intergenic 25.0%
! ATGTCTATAAGATATATAGG+TGG + chr6.2:24230110-24230129 MS.gene35994:CDS 25.0%
! CTTTAGTCATTTCAACATTA+TGG + chr6.2:24231557-24231576 MS.gene35994:intron 25.0%
! TAAAGAAAAACTAGAGGAAA+GGG + chr6.2:24230042-24230061 MS.gene35994:CDS 25.0%
! TAAAGAATCAAACAAGTGAA+TGG - chr6.2:24231542-24231561 None:intergenic 25.0%
! TGAAATTTGAATGAATGTTC+TGG + chr6.2:24230617-24230636 MS.gene35994:intron 25.0%
! TGAATAAATGAAAAGCAAGA+TGG - chr6.2:24231696-24231715 None:intergenic 25.0%
! TGGATATCATAGTAAAAATC+TGG - chr6.2:24230424-24230443 None:intergenic 25.0%
! TGTCTATAAGATATATAGGT+GGG + chr6.2:24230111-24230130 MS.gene35994:CDS 25.0%
! TTCCTTCAAATACTATTATG+AGG + chr6.2:24231731-24231750 MS.gene35994:intron 25.0%
! TTTCGATGAGTTAACTAATT+TGG + chr6.2:24231290-24231309 MS.gene35994:intron 25.0%
! TTTCTATTTACACAACACAA+TGG + chr6.2:24230939-24230958 MS.gene35994:intron 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG + chr6.2:24230719-24230738 MS.gene35994:intron 25.0%
!! ATTTTCAATTCTGAGTTAAG+TGG + chr6.2:24232825-24232844 MS.gene35994:intron 25.0%
!! CAAAGTTTTTACAATTGAGA+AGG + chr6.2:24231791-24231810 MS.gene35994:intron 25.0%
!! CTATACATTTTCACATTTGT+TGG + chr6.2:24232694-24232713 MS.gene35994:intron 25.0%
!! TGAGGCTTTAATAATTTAAG+CGG + chr6.2:24232520-24232539 MS.gene35994:intron 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG + chr6.2:24230980-24230999 MS.gene35994:intron 25.0%
!! TTGGTTTGAATATTATCTTC+TGG + chr6.2:24232668-24232687 MS.gene35994:intron 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr6.2:24230458-24230477 MS.gene35994:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG + chr6.2:24230459-24230478 MS.gene35994:CDS 25.0%
!!! ATGTTGATAATGATAATGAG+AGG + chr6.2:24231832-24231851 MS.gene35994:intron 25.0%
!!! CTTATTTTGTGAATTGTTCA+TGG - chr6.2:24229897-24229916 None:intergenic 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG + chr6.2:24230223-24230242 MS.gene35994:CDS 25.0%
!!! TGTTGATAATGATAATGAGA+GGG + chr6.2:24231833-24231852 MS.gene35994:intron 25.0%
AAACACATATGATCTTTCCA+TGG - chr6.2:24230790-24230809 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr6.2:24229953-24229972 MS.gene35994:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr6.2:24230553-24230572 MS.gene35994:CDS 30.0%
AAGAAAGACCAAATCCAATT+AGG + chr6.2:24232411-24232430 MS.gene35994:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG + chr6.2:24231331-24231350 MS.gene35994:intron 30.0%
AGAACATCTTATGAAGTGAT+GGG + chr6.2:24230256-24230275 MS.gene35994:CDS 30.0%
ATAAGTTAGGAAAGATGATG+AGG + chr6.2:24232564-24232583 MS.gene35994:intron 30.0%
ATCAGATTTCGACTACAATT+GGG + chr6.2:24231332-24231351 MS.gene35994:intron 30.0%
ATGATTGTCAAAACCAAGAA+AGG - chr6.2:24231025-24231044 None:intergenic 30.0%
ATTATGATCTTCAAACCTTG+GGG - chr6.2:24231904-24231923 None:intergenic 30.0%
CAACAAGATTATGCTCTATT+TGG + chr6.2:24230496-24230515 MS.gene35994:CDS 30.0%
CTAAAGAAAAACTAGAGGAA+AGG + chr6.2:24230041-24230060 MS.gene35994:CDS 30.0%
CTCCTCATAATAGTATTTGA+AGG - chr6.2:24231736-24231755 None:intergenic 30.0%
CTGGTATATGTGTGTTAATT+TGG + chr6.2:24230656-24230675 MS.gene35994:intron 30.0%
CTTCAAATACTATTATGAGG+AGG + chr6.2:24231734-24231753 MS.gene35994:intron 30.0%
GAAAGCTAAAGAAAAACTAG+AGG + chr6.2:24230036-24230055 MS.gene35994:CDS 30.0%
GAGAATGCAAAGAAAAATCT+CGG + chr6.2:24231246-24231265 MS.gene35994:intron 30.0%
GATTATGATCTTCAAACCTT+GGG - chr6.2:24231905-24231924 None:intergenic 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr6.2:24229952-24229971 MS.gene35994:CDS 30.0%
TGGTGTATACTTGTTTACTA+CGG - chr6.2:24231676-24231695 None:intergenic 30.0%
TTCTTTGACCTAATTGGATT+TGG - chr6.2:24232422-24232441 None:intergenic 30.0%
TTTAAGTCCGAAAATCGAAA+AGG - chr6.2:24230310-24230329 None:intergenic 30.0%
! AAATGCTCTGGTTTATGTAA+TGG - chr6.2:24230154-24230173 None:intergenic 30.0%
! AGCTGGAATATATTTGAAGT+TGG + chr6.2:24230075-24230094 MS.gene35994:CDS 30.0%
! GAATCTTCCTTTTCGATTTT+CGG + chr6.2:24230300-24230319 MS.gene35994:CDS 30.0%
! GAGGCTTTAATAATTTAAGC+GGG + chr6.2:24232521-24232540 MS.gene35994:intron 30.0%
! GCATCTGAAATATTTCTCAA+CGG + chr6.2:24232248-24232267 MS.gene35994:CDS 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG - chr6.2:24231139-24231158 None:intergenic 30.0%
! TCAATTCCGAATGTTTTTCA+AGG + chr6.2:24231189-24231208 MS.gene35994:CDS 30.0%
! TGAGGCTTTAACTAAAAAAG+CGG + chr6.2:24232582-24232601 MS.gene35994:intron 30.0%
! TTTGGTCTTTCTTTGAACTT+TGG - chr6.2:24232404-24232423 None:intergenic 30.0%
!! ATCATAATCGACTTGATGAA+AGG + chr6.2:24231916-24231935 MS.gene35994:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG + chr6.2:24230631-24230650 MS.gene35994:intron 30.0%
!! GAGGAGTTTCTTATTTCTAA+AGG + chr6.2:24232296-24232315 MS.gene35994:CDS 30.0%
!! TCATAATCGACTTGATGAAA+GGG + chr6.2:24231917-24231936 MS.gene35994:CDS 30.0%
!! TCGACATGTTTTCAAGATTT+TGG + chr6.2:24232013-24232032 MS.gene35994:CDS 30.0%
!!! CTGCACAATATGTTTTAAAG+CGG + chr6.2:24232081-24232100 MS.gene35994:CDS 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG + chr6.2:24231201-24231220 MS.gene35994:intron 30.0%
!!! TTCAAGATTTTGGAGTTCTT+AGG + chr6.2:24232023-24232042 MS.gene35994:CDS 30.0%
AATCAAACAAGTGAATGGTC+TGG - chr6.2:24231537-24231556 None:intergenic 35.0%
ACATTATGGTTCAGAGAATG+AGG + chr6.2:24231571-24231590 MS.gene35994:intron 35.0%
CATTATGGTTCAGAGAATGA+GGG + chr6.2:24231572-24231591 MS.gene35994:intron 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr6.2:24230193-24230212 MS.gene35994:CDS 35.0%
CGATTATGATCTTCAAACCT+TGG - chr6.2:24231906-24231925 None:intergenic 35.0%
CGTTATGCTTGTAACAAAGA+AGG + chr6.2:24229941-24229960 MS.gene35994:CDS 35.0%
CTCATTCAAATCGTATGCAT+TGG + chr6.2:24231418-24231437 MS.gene35994:CDS 35.0%
CTGAAATCTACCATGAATGT+TGG + chr6.2:24232137-24232156 MS.gene35994:CDS 35.0%
GAAGCAAAAGAAAGATGATG+AGG + chr6.2:24232502-24232521 MS.gene35994:intron 35.0%
GACAGAAATTCCTTATAGCT+CGG + chr6.2:24232477-24232496 MS.gene35994:intron 35.0%
GAGAACATCTTATGAAGTGA+TGG + chr6.2:24230255-24230274 MS.gene35994:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG + chr6.2:24229978-24229997 MS.gene35994:CDS 35.0%
TGTACAGAGCACAAATTCTA+AGG - chr6.2:24230766-24230785 None:intergenic 35.0%
TTCTCATACTCAACAAGAAC+TGG - chr6.2:24230019-24230038 None:intergenic 35.0%
! CATCGTAAGAGTGTGTTATT+TGG + chr6.2:24231042-24231061 MS.gene35994:CDS 35.0%
! GGTTTCGAATGTTGATGTTT+TGG + chr6.2:24231854-24231873 MS.gene35994:intron 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr6.2:24231501-24231520 MS.gene35994:intron 35.0%
! TGGTTTAGAATTGAAGGTTC+TGG + chr6.2:24230637-24230656 MS.gene35994:intron 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG + chr6.2:24231214-24231233 MS.gene35994:intron 35.0%
!! ACTGCTTTTGAAGACTTAGA+AGG - chr6.2:24232350-24232369 None:intergenic 35.0%
!! CACAAATTCTAAGGCAAGAT+AGG - chr6.2:24230757-24230776 None:intergenic 35.0%
!! CGCTTTAAAACATATTGTGC+AGG - chr6.2:24232083-24232102 None:intergenic 35.0%
!! TATGCATTGGTCTTCAGTTT+GGG + chr6.2:24231431-24231450 MS.gene35994:CDS 35.0%
!!! AAGATTTTGGAGTTCTTAGG+TGG + chr6.2:24232026-24232045 MS.gene35994:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG + chr6.2:24231085-24231104 MS.gene35994:CDS 35.0%
!!! CACAATATGTTTTAAAGCGG+TGG + chr6.2:24232084-24232103 MS.gene35994:CDS 35.0%
ACCTATGATCTTTGCATAGC+TGG - chr6.2:24230888-24230907 None:intergenic 40.0%
AGTCGCTTCTTTGACCTAAT+TGG - chr6.2:24232428-24232447 None:intergenic 40.0%
ATGAGGAGGACACAACAAAA+GGG + chr6.2:24231748-24231767 MS.gene35994:intron 40.0%
ATTTCTCAACGGTGTTCTTG+AGG + chr6.2:24232259-24232278 MS.gene35994:CDS 40.0%
GAAAAACTAGAGGAAAGGGT+TGG + chr6.2:24230046-24230065 MS.gene35994:CDS 40.0%
GACCTACTTCAGACTTTAAG+TGG + chr6.2:24231385-24231404 MS.gene35994:CDS 40.0%
GATTTCAGCTTATCGACAGA+CGG - chr6.2:24232125-24232144 None:intergenic 40.0%
GTCTTTAGTGCCAACATTCA+TGG - chr6.2:24232150-24232169 None:intergenic 40.0%
TACATTTCAGCTGCCTTTCT+TGG + chr6.2:24231009-24231028 MS.gene35994:intron 40.0%
TATGAGGAGGACACAACAAA+AGG + chr6.2:24231747-24231766 MS.gene35994:intron 40.0%
TCAAACCATATACACAGACC+AGG + chr6.2:24231149-24231168 MS.gene35994:CDS 40.0%
TCCAGCTATGCAAAGATCAT+AGG + chr6.2:24230884-24230903 MS.gene35994:intron 40.0%
TCTAATGACGACTCGTCAAA+AGG + chr6.2:24230336-24230355 MS.gene35994:CDS 40.0%
TGGTCTGTGTATATGGTTTG+AGG - chr6.2:24231150-24231169 None:intergenic 40.0%
TTCCCAATAGTCAGCTTATG+CGG + chr6.2:24231649-24231668 MS.gene35994:intron 40.0%
TTGATATCGTTGTGCTTGAG+TGG - chr6.2:24232174-24232193 None:intergenic 40.0%
TTTGCTTCTTCCGAGCTATA+AGG - chr6.2:24232490-24232509 None:intergenic 40.0%
! AGCCACTTAAAGTCTGAAGT+AGG - chr6.2:24231390-24231409 None:intergenic 40.0%
! GAGGGATAATCATGCTGATT+TGG + chr6.2:24231590-24231609 MS.gene35994:intron 40.0%
! TGAAGACTTAGAAGGCGTAA+CGG - chr6.2:24232342-24232361 None:intergenic 40.0%
!! GTATGCATTGGTCTTCAGTT+TGG + chr6.2:24231430-24231449 MS.gene35994:CDS 40.0%
!! GTTTTGGATGCAGAACTTGA+AGG + chr6.2:24231870-24231889 MS.gene35994:intron 40.0%
!! TCACATGTTGGCCTGTTTTT+AGG - chr6.2:24229992-24230011 None:intergenic 40.0%
!! TCATGCTGATTTGGACTTCA+AGG + chr6.2:24231599-24231618 MS.gene35994:intron 40.0%
!! TCTTATGAAGTGATGGGTCA+AGG + chr6.2:24230262-24230281 MS.gene35994:CDS 40.0%
!!! GCTAGTATTTTTTGGGCAGA+TGG + chr6.2:24230466-24230485 MS.gene35994:CDS 40.0%
ACGTCGTATGTTTGAGAACC+GGG + chr6.2:24231980-24231999 MS.gene35994:CDS 45.0%
AGAACTGGACTGTCACATGT+TGG - chr6.2:24230004-24230023 None:intergenic 45.0%
ATTTGTGCTCTGTACAGCCA+TGG + chr6.2:24230770-24230789 MS.gene35994:intron 45.0%
CACGTCGTATGTTTGAGAAC+CGG + chr6.2:24231979-24231998 MS.gene35994:CDS 45.0%
CATGTCGACATAAGAAACCC+CGG - chr6.2:24232001-24232020 None:intergenic 45.0%
CTCAAGTAACGCAAATGCTC+TGG - chr6.2:24230166-24230185 None:intergenic 45.0%
GACCGCATAAGCTGACTATT+GGG - chr6.2:24231654-24231673 None:intergenic 45.0%
GATCTTCAAACCTTGGGGAA+AGG - chr6.2:24231899-24231918 None:intergenic 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr6.2:24230444-24230463 None:intergenic 45.0%
TGTTCTTGAGGCAGGGAAAA+AGG + chr6.2:24232271-24232290 MS.gene35994:CDS 45.0%
TTGAAGGAAACCTTTCCCCA+AGG + chr6.2:24231886-24231905 MS.gene35994:CDS 45.0%
! GTGAGGTCACATTTTACAGC+TGG + chr6.2:24231453-24231472 MS.gene35994:CDS 45.0%
! TTTGGTGCAGTACCAACAGA+TGG - chr6.2:24232386-24232405 None:intergenic 45.0%
!! ATTGGTCTTCAGTTTGGGTG+AGG + chr6.2:24231436-24231455 MS.gene35994:CDS 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr6.2:24230058-24230077 MS.gene35994:CDS 45.0%
ACCAGCCTGGTCTGTGTATA+TGG - chr6.2:24231157-24231176 None:intergenic 50.0%
ACCATATACACAGACCAGGC+TGG + chr6.2:24231153-24231172 MS.gene35994:CDS 50.0%
CGCAAACACGTGTAGAAAGC+TGG - chr6.2:24232217-24232236 None:intergenic 50.0%
CGTCGTATGTTTGAGAACCG+GGG + chr6.2:24231981-24232000 MS.gene35994:CDS 50.0%
GACGACTCGTCAAAAGGAGA+TGG + chr6.2:24230342-24230361 MS.gene35994:CDS 50.0%
GACTCGTCAAAAGGAGATGG+TGG + chr6.2:24230345-24230364 MS.gene35994:CDS 50.0%
GCTGTCTGTGAACCATCTGT+TGG + chr6.2:24232371-24232390 MS.gene35994:CDS 50.0%
GGACCGCATAAGCTGACTAT+TGG - chr6.2:24231655-24231674 None:intergenic 50.0%
GGGTTGTCACAGTCGACATT+AGG + chr6.2:24231937-24231956 MS.gene35994:CDS 50.0%
GGTCTTTGCTCATGGCACAT+GGG + chr6.2:24231222-24231241 MS.gene35994:intron 50.0%
TACACGTGTTTGCGCAGATG+CGG + chr6.2:24232223-24232242 MS.gene35994:CDS 50.0%
TCAACGGTGTTCTTGAGGCA+GGG + chr6.2:24232264-24232283 MS.gene35994:CDS 50.0%
TGGTCTTTGCTCATGGCACA+TGG + chr6.2:24231221-24231240 MS.gene35994:intron 50.0%
!! GAAGTGATGGGTCAAGGAGT+TGG + chr6.2:24230268-24230287 MS.gene35994:CDS 50.0%
ACTTCAACAACGCACCAGCC+TGG - chr6.2:24231170-24231189 None:intergenic 55.0%
CTCAACGGTGTTCTTGAGGC+AGG + chr6.2:24232263-24232282 MS.gene35994:CDS 55.0%
TGAGGCAGGGAAAAAGGCAG+AGG + chr6.2:24232277-24232296 MS.gene35994:CDS 55.0%
! CGTCAAAAGGAGATGGTGGC+TGG + chr6.2:24230349-24230368 MS.gene35994:CDS 55.0%
!! GTGATGGGTCAAGGAGTTGG+AGG + chr6.2:24230271-24230290 MS.gene35994:CDS 55.0%
ACGTGTTTGCGCAGATGCGG+AGG + chr6.2:24232226-24232245 MS.gene35994:CDS 60.0%
GGGTCAAGGAGTTGGAGGCA+CGG + chr6.2:24230276-24230295 MS.gene35994:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 24229884 24232891 24229884 ID=MS.gene35994
chr6.2 mRNA 24229884 24232891 24229884 ID=MS.gene35994.t1;Parent=MS.gene35994
chr6.2 exon 24229884 24230574 24229884 ID=MS.gene35994.t1.exon1;Parent=MS.gene35994.t1
chr6.2 CDS 24229884 24230574 24229884 ID=cds.MS.gene35994.t1;Parent=MS.gene35994.t1
chr6.2 exon 24231019 24231210 24231019 ID=MS.gene35994.t1.exon2;Parent=MS.gene35994.t1
chr6.2 CDS 24231019 24231210 24231019 ID=cds.MS.gene35994.t1;Parent=MS.gene35994.t1
chr6.2 exon 24231337 24231495 24231337 ID=MS.gene35994.t1.exon3;Parent=MS.gene35994.t1
chr6.2 CDS 24231337 24231495 24231337 ID=cds.MS.gene35994.t1;Parent=MS.gene35994.t1
chr6.2 exon 24231883 24232466 24231883 ID=MS.gene35994.t1.exon4;Parent=MS.gene35994.t1
chr6.2 CDS 24231883 24232466 24231883 ID=cds.MS.gene35994.t1;Parent=MS.gene35994.t1
chr6.2 exon 24232889 24232891 24232889 ID=MS.gene35994.t1.exon5;Parent=MS.gene35994.t1
chr6.2 CDS 24232889 24232891 24232889 ID=cds.MS.gene35994.t1;Parent=MS.gene35994.t1
Gene Sequence

>MS.gene35994

ATGTTGAAGTCCATGAACAATTCACAAAATAAGAAATCAGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACTAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGTGTTGAATGTCTATAAGATATATAGGTGGGAAGTTGAGCACTGTCATCCATTACATAAACCAGAGCATTTGCGTTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACGACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGATTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTATCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGTTCTGGTATATGTGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATTGGTGATCCTATCTTGCCTTAGAATTTGTGCTCTGTACAGCCATGGAAAGATCATATGTGTTTTTTTATCGATGATAAATAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTTACAGTTGTTAAATGAATAGCTTTTTCTATTTACACAACACAATGGTTTTATATTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTACATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATTAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGACCTACTTCAGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAATGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTTGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGATTTGGACTTCAAGGCTGCAAACACTAGAACAAAAAATAATTTTCCCAATAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACATTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTTACAATTGAGAAGGTTGATTATAATTACGACGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAACTTGAAGGAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGCAAAAATTTTACTTGTTCACGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGTTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCTACCATGAATGTTGGCACTAAAGACACCACTCAAGCACAACGATATCAACAAATTTGTGTTGTTACAATCCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAACCATCTGTTGGTACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGTTAGGGCAAGACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGCTTTAATAATTTAAGCGGGCACTGATTGTTTTTTATGTTATAAGTTAGGAAAGATGATGAGGCTTTAACTAAAAAAGCGGTCACTTATGTTATATGTGATAAGTTTGTACTAGATTTTATATCAAATTCTTGATTGTTATGTATTGGTTTGAATATTATCTTCTGGAAACTATACATTTTCACATTTGTTGGTTTTTACGTATTTATTATTATATTTTGGTACAAAACCATTAGATTGTTAATTTGGTTTCATATAGTACACTATCAAAACCATTAAACCATAATTATGGTTTAGTTAAGATTTTCAATTCTGAGTTAAGTGGATATATTACTGAGTTTTGCAATTTGCTTCAACCTATTCAAGTGA
Protein sequence

>MS.gene35994.t1

MLKSMNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKLEERVGCKAGIYLKLDEVLNVYKIYRWEVEHCHPLHKPEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPDFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKMTQLKLEGNLSPRFEDHNRLDERVVTVDIRSKNFTCSRRMFENRGFLCRHVFKILEFLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTQAQRYQQICVVTIQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVCEPSVGTAPKFKERPNPIRSKKRLKSDYEK