AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36133

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36133.t1 MTR_8g442410 95.522 536 24 0 6 541 1 536 0.0 1048
MS.gene36133.t1 MTR_4g104020 47.135 384 190 7 154 530 143 520 1.38e-103 321
MS.gene36133.t1 MTR_7g027190 41.814 397 194 6 154 530 294 673 1.33e-93 300
MS.gene36133.t1 MTR_3g065980 35.678 398 214 11 154 531 166 541 1.34e-76 252
MS.gene36133.t1 MTR_5g094450 34.625 387 230 7 156 530 157 532 4.15e-66 224
MS.gene36133.t1 MTR_3g089055 34.656 378 228 6 161 530 204 570 9.74e-66 223
MS.gene36133.t1 MTR_3g089055 34.656 378 228 6 161 530 199 565 1.08e-65 223
MS.gene36133.t1 MTR_5g097480 33.854 384 233 8 156 530 173 544 2.36e-64 219
MS.gene36133.t1 MTR_1g029420 34.392 378 229 6 161 530 226 592 9.88e-64 219
MS.gene36133.t1 MTR_2g082090 36.061 391 215 9 156 530 208 579 1.22e-63 218
MS.gene36133.t1 MTR_3g056110 33.073 384 236 7 156 530 171 542 2.25e-63 216
MS.gene36133.t1 MTR_4g064150 30.900 411 250 8 140 532 341 735 6.50e-59 208
MS.gene36133.t1 MTR_7g074650 36.043 369 216 8 164 530 442 792 1.68e-58 208
MS.gene36133.t1 MTR_4g133660 33.162 389 230 8 155 529 170 542 9.61e-58 201
MS.gene36133.t1 MTR_4g064160 31.078 399 250 8 148 532 299 686 1.60e-57 204
MS.gene36133.t1 MTR_4g102790 32.990 388 240 11 152 529 284 661 1.86e-57 203
MS.gene36133.t1 MTR_4g064120 32.821 390 235 10 152 527 246 622 1.85e-56 200
MS.gene36133.t1 MTR_4g064180 31.771 384 247 6 152 527 246 622 2.78e-56 199
MS.gene36133.t1 MTR_2g097410 31.250 384 248 5 154 529 365 740 2.27e-54 196
MS.gene36133.t1 MTR_4g064200 31.510 384 241 7 155 529 279 649 1.06e-53 192
MS.gene36133.t1 MTR_2g097467 29.948 384 248 6 154 529 283 653 1.94e-52 189
MS.gene36133.t1 MTR_4g076020 35.263 380 212 11 164 538 88 438 3.73e-52 184
MS.gene36133.t1 MTR_2g097310 28.676 408 266 10 134 532 249 640 9.54e-52 187
MS.gene36133.t1 MTR_2g097463 30.311 386 248 6 155 532 285 657 1.83e-51 186
MS.gene36133.t1 MTR_2g097473 29.457 387 252 6 154 532 283 656 2.66e-50 183
MS.gene36133.t1 MTR_2g097390 29.949 394 234 10 155 529 270 640 3.19e-50 183
MS.gene36133.t1 MTR_2g097350 30.127 395 232 12 155 529 269 639 1.34e-49 181
MS.gene36133.t1 MTR_7g062120 29.648 398 260 6 144 532 356 742 1.48e-49 182
MS.gene36133.t1 MTR_2g026250 30.130 385 249 7 155 530 223 596 3.14e-49 179
MS.gene36133.t1 MTR_1g069725 33.590 390 219 11 152 529 101 462 3.25e-49 177
MS.gene36133.t1 MTR_2g097380 28.184 369 224 8 165 529 229 560 5.53e-45 167
MS.gene36133.t1 MTR_2g034250 31.105 389 251 10 153 530 203 585 6.82e-45 167
MS.gene36133.t1 MTR_2g034280 31.472 394 240 11 153 530 196 575 9.23e-45 167
MS.gene36133.t1 MTR_3g022830 28.738 428 230 11 161 530 26 436 1.15e-44 164
MS.gene36133.t1 MTR_3g072710 33.007 409 230 15 152 533 112 503 1.04e-43 162
MS.gene36133.t1 MTR_5g015490 28.534 382 255 7 161 530 115 490 3.08e-42 158
MS.gene36133.t1 MTR_4g097080 28.684 380 251 11 161 530 134 503 3.91e-41 155
MS.gene36133.t1 MTR_5g058860 30.941 404 241 13 153 533 112 500 4.41e-41 155
MS.gene36133.t1 MTR_2g034260 29.825 399 242 11 153 530 203 584 2.63e-40 154
MS.gene36133.t1 MTR_7g057230 31.361 338 209 7 200 529 334 656 1.62e-39 153
MS.gene36133.t1 MTR_4g076140 45.270 148 79 1 385 530 322 469 2.09e-37 144
MS.gene36133.t1 MTR_4g076140 34.667 225 125 7 153 372 41 248 6.74e-28 117
MS.gene36133.t1 MTR_1g086970 28.155 412 258 13 139 530 85 478 2.11e-37 144
MS.gene36133.t1 MTR_1g096030 29.369 412 230 17 154 530 74 459 2.52e-37 144
MS.gene36133.t1 MTR_5g009080 43.421 152 84 1 382 531 328 479 1.24e-36 142
MS.gene36133.t1 MTR_5g009080 32.895 228 125 7 153 372 49 256 4.78e-27 114
MS.gene36133.t1 MTR_4g095500 27.404 416 247 11 161 529 60 467 1.37e-33 133
MS.gene36133.t1 MTR_6g047750 29.897 388 237 12 159 530 294 662 1.41e-33 135
MS.gene36133.t1 MTR_4g077760 28.571 385 245 10 157 530 190 555 6.11e-33 132
MS.gene36133.t1 MTR_2g089100 27.685 419 254 9 146 529 38 442 4.36e-32 129
MS.gene36133.t1 MTR_2g099110 28.462 390 246 10 161 530 75 451 1.38e-31 127
MS.gene36133.t1 MTR_5g015950 26.777 422 244 16 161 529 36 445 3.67e-31 126
MS.gene36133.t1 MTR_3g053270 26.777 422 244 16 161 529 36 445 3.67e-31 126
MS.gene36133.t1 MTR_7g109580 26.154 390 261 12 152 530 181 554 1.97e-27 116
MS.gene36133.t1 MTR_8g093070 26.329 395 256 13 157 540 136 506 1.08e-25 110
MS.gene36133.t1 MTR_0092s0100 24.870 386 252 10 157 530 372 731 1.46e-22 102
MS.gene36133.t1 MTR_0092s0100 24.870 386 252 10 157 530 369 728 1.52e-22 102
MS.gene36133.t1 MTR_4g026485 23.426 397 261 12 147 530 258 624 1.28e-18 89.7
MS.gene36133.t1 MTR_8g020840 22.362 398 262 13 161 531 172 549 8.47e-16 80.9
MS.gene36133.t1 MTR_3g022580 35.385 130 74 4 409 530 56 183 1.40e-15 75.5
MS.gene36133.t1 MTR_8g077940 23.724 392 254 15 156 530 178 541 3.92e-15 78.6
MS.gene36133.t1 MTR_3g022005 37.634 93 55 2 440 530 34 125 1.17e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36133.t1 AT1G66350 38.120 383 208 11 154 531 149 507 4.16e-77 252
MS.gene36133.t1 AT3G03450 37.403 385 221 10 154 532 177 547 1.12e-75 249
MS.gene36133.t1 AT1G14920 37.726 387 214 11 154 533 166 532 3.60e-73 242
MS.gene36133.t1 AT2G01570 36.410 390 221 11 154 536 218 587 1.54e-72 242
MS.gene36133.t1 AT5G17490 31.161 491 293 12 69 533 48 519 4.64e-67 226
MS.gene36133.t1 AT2G04890 34.375 384 224 7 156 530 49 413 1.22e-63 214
MS.gene36133.t1 AT3G54220 37.736 371 208 8 164 529 297 649 4.30e-62 216
MS.gene36133.t1 AT5G48150 33.159 383 238 6 156 530 118 490 1.33e-61 211
MS.gene36133.t1 AT5G48150 33.159 383 238 6 156 530 118 490 1.33e-61 211
MS.gene36133.t1 AT5G48150 33.159 383 238 6 156 530 118 490 1.33e-61 211
MS.gene36133.t1 AT5G48150 33.159 383 238 6 156 530 118 490 1.33e-61 211
MS.gene36133.t1 AT1G21450 32.895 380 237 5 159 530 224 593 2.01e-60 210
MS.gene36133.t1 AT1G50600 33.333 384 235 7 156 530 155 526 3.75e-59 205
MS.gene36133.t1 AT1G50600 33.333 384 235 7 156 530 226 597 1.46e-58 205
MS.gene36133.t1 AT1G50600 33.333 384 235 7 156 530 230 601 1.91e-58 205
MS.gene36133.t1 AT5G41920 35.000 380 228 9 154 530 37 400 9.03e-58 198
MS.gene36133.t1 AT1G07530 28.802 434 287 8 110 533 347 768 2.80e-57 204
MS.gene36133.t1 AT4G17230 33.503 394 235 8 149 530 147 525 1.98e-53 189
MS.gene36133.t1 AT4G17230 33.503 394 235 8 149 530 147 525 1.98e-53 189
MS.gene36133.t1 AT2G29060 31.095 402 254 7 140 529 264 654 2.43e-53 192
MS.gene36133.t1 AT2G29060 31.095 402 254 7 140 529 301 691 5.94e-53 191
MS.gene36133.t1 AT2G29065 27.423 485 319 12 65 529 156 627 5.53e-52 187
MS.gene36133.t1 AT1G07520 27.293 458 291 9 85 529 100 528 1.34e-50 182
MS.gene36133.t1 AT1G07520 27.293 458 291 9 85 529 100 528 1.34e-50 182
MS.gene36133.t1 AT1G07520 27.293 458 291 9 85 529 265 693 9.67e-50 182
MS.gene36133.t1 AT1G07520 27.293 458 291 9 85 529 264 692 1.02e-49 182
MS.gene36133.t1 AT5G59450 28.176 433 284 10 111 532 184 600 3.68e-48 177
MS.gene36133.t1 AT1G63100 31.006 358 231 7 151 503 268 614 2.99e-47 175
MS.gene36133.t1 AT1G63100 31.006 358 231 7 151 503 243 589 3.41e-47 174
MS.gene36133.t1 AT1G55580 32.181 376 218 10 189 530 73 445 3.65e-47 171
MS.gene36133.t1 AT3G46600 29.008 393 252 8 152 532 204 581 9.25e-47 172
MS.gene36133.t1 AT2G37650 28.571 392 260 6 150 533 325 704 1.78e-46 173
MS.gene36133.t1 AT3G46600 29.008 393 252 8 152 532 172 549 2.13e-46 171
MS.gene36133.t1 AT3G46600 29.091 385 246 8 161 533 5 374 2.21e-46 167
MS.gene36133.t1 AT2G37650 28.571 392 260 6 150 533 337 716 2.38e-46 173
MS.gene36133.t1 AT4G37650 28.049 410 269 11 141 530 126 529 1.62e-42 159
MS.gene36133.t1 AT4G08250 29.668 391 249 14 152 529 99 476 1.74e-38 147
MS.gene36133.t1 AT5G66770 29.534 386 242 10 157 530 217 584 8.87e-38 147
MS.gene36133.t1 AT3G50650 29.888 358 218 9 188 530 203 542 4.74e-37 144
MS.gene36133.t1 AT5G52510 31.548 336 205 8 217 530 308 640 6.83e-37 145
MS.gene36133.t1 AT1G50420 43.137 153 85 1 380 530 327 479 9.41e-35 137
MS.gene36133.t1 AT1G50420 32.889 225 137 4 153 375 50 262 7.37e-30 123
MS.gene36133.t1 AT3G49950 28.212 397 237 15 161 529 31 407 3.44e-32 128
MS.gene36133.t1 AT3G60630 26.710 307 201 10 238 530 326 622 1.53e-21 99.0
MS.gene36133.t1 AT4G00150 27.887 355 218 12 186 533 234 557 3.10e-19 91.7
MS.gene36133.t1 AT4G36710 23.018 391 267 12 152 530 117 485 7.62e-18 87.0
MS.gene36133.t1 AT2G45160 24.225 516 317 21 54 530 159 639 1.10e-17 87.0
MS.gene36133.t1 AT3G13840 23.383 402 258 15 150 530 138 510 2.66e-15 79.0

Find 101 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAAATGAACGGCAATTT+TGG 0.207673 8.3:-51827877 MS.gene36133:CDS
TCAATACATATAATTGATTT+AGG 0.211372 8.3:-51827460 MS.gene36133:CDS
ATCCTAATTGGACTATTTCT+TGG 0.252505 8.3:+51826974 None:intergenic
TTCCTTTCCAACCAAGTAAA+AGG 0.254662 8.3:+51826735 None:intergenic
TGATCTTGCTCCATTCTTGC+AGG 0.255135 8.3:+51828195 None:intergenic
CACAATGGACCTTTCTTTCT+TGG 0.260406 8.3:-51827058 MS.gene36133:CDS
TTTGTTACTTGGTAAAATTT+TGG 0.264110 8.3:-51827743 MS.gene36133:CDS
TTCAATGGCCTTCACTTATA+AGG 0.274914 8.3:-51827423 MS.gene36133:CDS
ACAACACCTTACATTACTTT+TGG 0.300835 8.3:-51827532 MS.gene36133:CDS
CCATTTCCTATTGTCTTAAG+AGG 0.321187 8.3:+51826655 None:intergenic
GATCAATGGAGAAGACAATT+AGG 0.324260 8.3:-51826863 MS.gene36133:CDS
TCATGTCTCTCCATTCTATC+AGG 0.340101 8.3:+51826893 None:intergenic
ATGATGTTTCTTTGATTACT+AGG 0.342089 8.3:-51827591 MS.gene36133:CDS
GACACTTTCATTCATATAGA+AGG 0.344185 8.3:+51828248 None:intergenic
TTGGCTTTGTCAAGTCCTTC+AGG 0.354507 8.3:-51827724 MS.gene36133:CDS
CCTCCAAAGCTCAGGATAAC+AGG 0.367635 8.3:-51827376 MS.gene36133:CDS
CCTGTTATCCTGAGCTTTGG+AGG 0.384703 8.3:+51827376 None:intergenic
GCAGGGCAAAACCTTCTGAT+AGG 0.390949 8.3:+51828213 None:intergenic
CTGAGCTTTGGAGGCCCCTC+AGG 0.392034 8.3:+51827385 None:intergenic
TTGGTGAATGATGATGTCTT+TGG 0.396171 8.3:-51827976 MS.gene36133:CDS
TGACTTGGTCCATACGCTAC+TGG 0.405114 8.3:-51827809 MS.gene36133:CDS
ATATACTTTGTCATGTGAAA+AGG 0.405685 8.3:-51826762 MS.gene36133:CDS
TGCCAAGAAATAGTCCAATT+AGG 0.408355 8.3:-51826976 MS.gene36133:CDS
TCTACAACTGTGAGTGCAAT+TGG 0.437872 8.3:+51827094 None:intergenic
AATCCTGTTATCCTGAGCTT+TGG 0.439914 8.3:+51827373 None:intergenic
AAGTAATGTAAGGTGTTGTT+TGG 0.443376 8.3:+51827536 None:intergenic
AATCAGGCGAATTTGTTACT+TGG 0.447598 8.3:-51827754 MS.gene36133:CDS
TGTAGAAGAAGCACTCTCAT+TGG 0.455081 8.3:-51827299 MS.gene36133:CDS
GACATGAAAGAGTAGATCAA+TGG 0.459154 8.3:-51826877 MS.gene36133:CDS
ATGGACCTTTCTTTCTTGGT+AGG 0.464231 8.3:-51827054 MS.gene36133:CDS
GCTATATGCCAAGGATCAAA+AGG 0.466005 8.3:-51827490 MS.gene36133:CDS
CTATTTGAAAATACATTATG+TGG 0.466093 8.3:+51827637 None:intergenic
GTAGAAGAAGCACTCTCATT+GGG 0.466957 8.3:-51827298 MS.gene36133:CDS
GAAAGGAAGGTCTATTATGA+TGG 0.467802 8.3:-51826720 MS.gene36133:CDS
ACCTTCTGATAGGGAAATCA+AGG 0.468486 8.3:+51828223 None:intergenic
GATCTTGCTCCATTCTTGCA+GGG 0.471751 8.3:+51828196 None:intergenic
GTAAGAAGAGAAGGAGTTGC+CGG 0.477608 8.3:+51827244 None:intergenic
CTGGCTTGTGCCGAGGCCGT+TGG 0.479503 8.3:-51827790 MS.gene36133:CDS
TGATGTTTCTTTGATTACTA+GGG 0.480820 8.3:-51827590 MS.gene36133:CDS
TCTTCATCCTCCATTGTGGT+TGG 0.481380 8.3:+51827946 None:intergenic
GCCTTGGTTCATACCGAGAC+TGG 0.481981 8.3:+51827834 None:intergenic
CACATGTTTAAGTCTTCCAT+TGG 0.485091 8.3:+51828276 None:intergenic
ATGAAACCAAAAGTAATGTA+AGG 0.486942 8.3:+51827526 None:intergenic
GATTACTAGGGAGAATAAGT+TGG 0.489189 8.3:-51827578 MS.gene36133:CDS
CATCATCAAGAACTGTCTCT+TGG 0.496418 8.3:+51828154 None:intergenic
TTGCCACAGCATTGAAGTGT+AGG 0.499786 8.3:-51827672 MS.gene36133:CDS
TGCAATATTTGATTCTCTTG+AGG 0.503760 8.3:-51827005 MS.gene36133:CDS
GTCTCTGCAACCAACGGCCT+CGG 0.503923 8.3:+51827780 None:intergenic
AGAAGACAATTAGGAAGAGC+AGG 0.503981 8.3:-51826854 MS.gene36133:CDS
CTGATATTATGCGAAATTCA+AGG 0.506362 8.3:+51827269 None:intergenic
TTATAAGGAACCTCGCTTCG+AGG 0.508266 8.3:-51827408 MS.gene36133:CDS
CCGAGACTGGCTTGTGATTG+TGG 0.515572 8.3:+51827847 None:intergenic
GCCCCTCAGGCCTCGAAGCG+AGG 0.518387 8.3:+51827398 None:intergenic
CTTTCTGTTTATGATTGTGA+TGG 0.518538 8.3:-51826785 MS.gene36133:CDS
CTATTATGATGGCTTCTGCT+TGG 0.519258 8.3:-51826709 MS.gene36133:CDS
TATACTTTGTCATGTGAAAA+GGG 0.523287 8.3:-51826761 MS.gene36133:CDS
CGGCACAAGCCAGTAGCGTA+TGG 0.524974 8.3:+51827800 None:intergenic
CTGAGGGGCCTCCAAAGCTC+AGG 0.525835 8.3:-51827384 MS.gene36133:CDS
GAATGGAGCATACTCTTCAA+TGG 0.527179 8.3:-51827438 MS.gene36133:CDS
TATGGACCAAGTCAAGGCCT+TGG 0.530054 8.3:+51827818 None:intergenic
AAGTCATCAACTTCTGAAGA+AGG 0.531009 8.3:+51828087 None:intergenic
AGAAACCTACCAAGAAAGAA+AGG 0.532154 8.3:+51827049 None:intergenic
AACCTCGCTTCGAGGCCTGA+GGG 0.533889 8.3:-51827400 MS.gene36133:CDS
ATTCACCTTCTCTCAAGTTG+AGG 0.536949 8.3:+51827212 None:intergenic
TTGTGAGGGTCCTGATAGAA+TGG 0.538774 8.3:-51826903 MS.gene36133:CDS
TTGCAACATTGTAGCTTGTG+AGG 0.544201 8.3:-51826918 MS.gene36133:CDS
ACCTTGATTTCCCTATCAGA+AGG 0.546601 8.3:-51828224 MS.gene36133:CDS
CAAGAACTGTCTCTTGGTCA+TGG 0.547218 8.3:+51828160 None:intergenic
AAATTCTTCATCCTCCATTG+TGG 0.547730 8.3:+51827942 None:intergenic
TGCAACATTGTAGCTTGTGA+GGG 0.548434 8.3:-51826917 MS.gene36133:CDS
CAATGCTGTGGCAAAGCAGT+AGG 0.548768 8.3:+51827681 None:intergenic
ATTTCGCATAATATCAGAGC+CGG 0.550299 8.3:-51827263 MS.gene36133:CDS
CAGGGCAAAACCTTCTGATA+GGG 0.552869 8.3:+51828214 None:intergenic
TGTTGATAGCTTCATCAACA+TGG 0.554822 8.3:-51828064 MS.gene36133:CDS
AGAAGGTAATAAACAAGGAC+TGG 0.559389 8.3:+51828104 None:intergenic
ACATATAATTGATTTAGGAA+TGG 0.566467 8.3:-51827455 MS.gene36133:CDS
TACGCTACTGGCTTGTGCCG+AGG 0.566964 8.3:-51827797 MS.gene36133:CDS
GAACCTCGCTTCGAGGCCTG+AGG 0.569227 8.3:-51827401 MS.gene36133:CDS
TGGTTGCAGAGACAACAATC+AGG 0.574071 8.3:-51827770 MS.gene36133:CDS
GACGAAGAAGTTGAAATGAA+CGG 0.577431 8.3:-51827886 MS.gene36133:CDS
GCTTGACTTGTACACTTGAG+TGG 0.583171 8.3:+51826818 None:intergenic
TATGAACCAAGGCCTTGACT+TGG 0.587577 8.3:-51827824 MS.gene36133:CDS
CCACAATCACAAGCCAGTCT+CGG 0.590657 8.3:-51827847 MS.gene36133:CDS
ACCTCGCTTCGAGGCCTGAG+GGG 0.592685 8.3:-51827399 MS.gene36133:CDS
GAAAACCTCAACTTGAGAGA+AGG 0.599776 8.3:-51827217 MS.gene36133:CDS
GATGGCTTCTGCTTGGCAAG+TGG 0.608972 8.3:-51826702 MS.gene36133:CDS
GCGAGGTTCCTTATAAGTGA+AGG 0.612978 8.3:+51827415 None:intergenic
GCAAATGAAGCTATATGCCA+AGG 0.622841 8.3:-51827499 MS.gene36133:CDS
ATTGTTGTCTCTGCAACCAA+CGG 0.634384 8.3:+51827774 None:intergenic
GCCAGTCTCGGTATGAACCA+AGG 0.638041 8.3:-51827835 MS.gene36133:CDS
CAATGGAAGACTTAAACATG+TGG 0.638349 8.3:-51828275 MS.gene36133:CDS
GTAGCGTATGGACCAAGTCA+AGG 0.641162 8.3:+51827812 None:intergenic
CTTTGTAACGAATCTCCTGA+AGG 0.642692 8.3:+51827709 None:intergenic
TAACCTACACTTCAATGCTG+TGG 0.647062 8.3:+51827669 None:intergenic
TGGATATGATCCAACCACAA+TGG 0.666133 8.3:-51827956 MS.gene36133:CDS
AAATATGTGAAAGAAAGTAG+AGG 0.666936 8.3:-51827157 MS.gene36133:CDS
CAAGAAAGAAAGGTCCATTG+TGG 0.670675 8.3:+51827059 None:intergenic
AGAATGGAGCAAGATCAAGA+AGG 0.671700 8.3:-51828189 MS.gene36133:CDS
ATATGATCCAACCACAATGG+AGG 0.672820 8.3:-51827953 MS.gene36133:CDS
GAGCAAGACACAAACCACAA+TGG 0.673924 8.3:-51827073 MS.gene36133:CDS
TCTGAAGAAGGTAATAAACA+AGG 0.680577 8.3:+51828099 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATACATATAATTGATTT+AGG - chr8.3:51827483-51827502 MS.gene36133:CDS 15.0%
!! CTATTTGAAAATACATTATG+TGG + chr8.3:51827309-51827328 None:intergenic 20.0%
!!! ACATATAATTGATTTAGGAA+TGG - chr8.3:51827488-51827507 MS.gene36133:CDS 20.0%
!!! TTTGTTACTTGGTAAAATTT+TGG - chr8.3:51827200-51827219 MS.gene36133:CDS 20.0%
! AAATATGTGAAAGAAAGTAG+AGG - chr8.3:51827786-51827805 MS.gene36133:CDS 25.0%
! ATATACTTTGTCATGTGAAA+AGG - chr8.3:51828181-51828200 MS.gene36133:CDS 25.0%
! ATGAAACCAAAAGTAATGTA+AGG + chr8.3:51827420-51827439 None:intergenic 25.0%
! ATGATGTTTCTTTGATTACT+AGG - chr8.3:51827352-51827371 MS.gene36133:CDS 25.0%
! TATACTTTGTCATGTGAAAA+GGG - chr8.3:51828182-51828201 MS.gene36133:CDS 25.0%
! TGATGTTTCTTTGATTACTA+GGG - chr8.3:51827353-51827372 MS.gene36133:CDS 25.0%
!!! AAGTTTTGATCATCACTTTT+TGG - chr8.3:51826948-51826967 MS.gene36133:CDS 25.0%
!!! TTACATTACTTTTGGTTTCA+TGG - chr8.3:51827419-51827438 MS.gene36133:CDS 25.0%
CTGATATTATGCGAAATTCA+AGG + chr8.3:51827677-51827696 None:intergenic 30.0%
CTTTCTGTTTATGATTGTGA+TGG - chr8.3:51828158-51828177 MS.gene36133:CDS 30.0%
GACACTTTCATTCATATAGA+AGG + chr8.3:51826698-51826717 None:intergenic 30.0%
TCTGAAGAAGGTAATAAACA+AGG + chr8.3:51826847-51826866 None:intergenic 30.0%
TGCAATATTTGATTCTCTTG+AGG - chr8.3:51827938-51827957 MS.gene36133:CDS 30.0%
! ACAACACCTTACATTACTTT+TGG - chr8.3:51827411-51827430 MS.gene36133:CDS 30.0%
! ATCCTAATTGGACTATTTCT+TGG + chr8.3:51827972-51827991 None:intergenic 30.0%
! CTCTCTATTTTCATCCTAAT+TGG + chr8.3:51827984-51828003 None:intergenic 30.0%
! TTTTCGATTGTAAGAAGAGA+AGG + chr8.3:51827711-51827730 None:intergenic 30.0%
!! AAGTAATGTAAGGTGTTGTT+TGG + chr8.3:51827410-51827429 None:intergenic 30.0%
AAATTCTTCATCCTCCATTG+TGG + chr8.3:51827004-51827023 None:intergenic 35.0%
AGAAACCTACCAAGAAAGAA+AGG + chr8.3:51827897-51827916 None:intergenic 35.0%
AGAAGGTAATAAACAAGGAC+TGG + chr8.3:51826842-51826861 None:intergenic 35.0%
ATTTCGCATAATATCAGAGC+CGG - chr8.3:51827680-51827699 MS.gene36133:CDS 35.0%
GAAAGGAAGGTCTATTATGA+TGG - chr8.3:51828223-51828242 MS.gene36133:CDS 35.0%
GACATGAAAGAGTAGATCAA+TGG - chr8.3:51828066-51828085 MS.gene36133:CDS 35.0%
GACGAAGAAGTTGAAATGAA+CGG - chr8.3:51827057-51827076 MS.gene36133:CDS 35.0%
GATCAATGGAGAAGACAATT+AGG - chr8.3:51828080-51828099 MS.gene36133:CDS 35.0%
GATTACTAGGGAGAATAAGT+TGG - chr8.3:51827365-51827384 MS.gene36133:CDS 35.0%
TGCCAAGAAATAGTCCAATT+AGG - chr8.3:51827967-51827986 MS.gene36133:CDS 35.0%
TTCAATGGCCTTCACTTATA+AGG - chr8.3:51827520-51827539 MS.gene36133:CDS 35.0%
TTCCTTTCCAACCAAGTAAA+AGG + chr8.3:51828211-51828230 None:intergenic 35.0%
! AAGTCATCAACTTCTGAAGA+AGG + chr8.3:51826859-51826878 None:intergenic 35.0%
! GTTGAAATGAACGGCAATTT+TGG - chr8.3:51827066-51827085 MS.gene36133:CDS 35.0%
! TTGGTGAATGATGATGTCTT+TGG - chr8.3:51826967-51826986 MS.gene36133:CDS 35.0%
! TTTTACTTGGTTGGAAAGGA+AGG - chr8.3:51828210-51828229 MS.gene36133:CDS 35.0%
!! AATCAGGCGAATTTGTTACT+TGG - chr8.3:51827189-51827208 MS.gene36133:CDS 35.0%
!! AGCTTTTTCCTTTTGATCCT+TGG + chr8.3:51827464-51827483 None:intergenic 35.0%
!! TGTTGATAGCTTCATCAACA+TGG - chr8.3:51826879-51826898 MS.gene36133:CDS 35.0%
AATCCTGTTATCCTGAGCTT+TGG + chr8.3:51827573-51827592 None:intergenic 40.0%
ACCTTCTGATAGGGAAATCA+AGG + chr8.3:51826723-51826742 None:intergenic 40.0%
ACCTTGATTTCCCTATCAGA+AGG - chr8.3:51826719-51826738 MS.gene36133:CDS 40.0%
AGAAGACAATTAGGAAGAGC+AGG - chr8.3:51828089-51828108 MS.gene36133:CDS 40.0%
AGAATGGAGCAAGATCAAGA+AGG - chr8.3:51826754-51826773 MS.gene36133:CDS 40.0%
ATATGATCCAACCACAATGG+AGG - chr8.3:51826990-51827009 MS.gene36133:CDS 40.0%
ATGGACCTTTCTTTCTTGGT+AGG - chr8.3:51827889-51827908 MS.gene36133:CDS 40.0%
ATTGTTGTCTCTGCAACCAA+CGG + chr8.3:51827172-51827191 None:intergenic 40.0%
CAAGAAAGAAAGGTCCATTG+TGG + chr8.3:51827887-51827906 None:intergenic 40.0%
CACAATGGACCTTTCTTTCT+TGG - chr8.3:51827885-51827904 MS.gene36133:CDS 40.0%
CATCATCAAGAACTGTCTCT+TGG + chr8.3:51826792-51826811 None:intergenic 40.0%
CTATTATGATGGCTTCTGCT+TGG - chr8.3:51828234-51828253 MS.gene36133:CDS 40.0%
CTTTGTAACGAATCTCCTGA+AGG + chr8.3:51827237-51827256 None:intergenic 40.0%
GAAAACCTCAACTTGAGAGA+AGG - chr8.3:51827726-51827745 MS.gene36133:CDS 40.0%
GAATGGAGCATACTCTTCAA+TGG - chr8.3:51827505-51827524 MS.gene36133:CDS 40.0%
GCAAATGAAGCTATATGCCA+AGG - chr8.3:51827444-51827463 MS.gene36133:CDS 40.0%
GCTATATGCCAAGGATCAAA+AGG - chr8.3:51827453-51827472 MS.gene36133:CDS 40.0%
TAACCTACACTTCAATGCTG+TGG + chr8.3:51827277-51827296 None:intergenic 40.0%
TCATGTCTCTCCATTCTATC+AGG + chr8.3:51828053-51828072 None:intergenic 40.0%
TCTACAACTGTGAGTGCAAT+TGG + chr8.3:51827852-51827871 None:intergenic 40.0%
TGGATATGATCCAACCACAA+TGG - chr8.3:51826987-51827006 MS.gene36133:CDS 40.0%
! ATTCACCTTCTCTCAAGTTG+AGG + chr8.3:51827734-51827753 None:intergenic 40.0%
! GAAAAGGGTTGCCTTTTACT+TGG - chr8.3:51828197-51828216 MS.gene36133:CDS 40.0%
! TGCAACATTGTAGCTTGTGA+GGG - chr8.3:51828026-51828045 MS.gene36133:CDS 40.0%
! TGCCTTTTACTTGGTTGGAA+AGG - chr8.3:51828206-51828225 MS.gene36133:CDS 40.0%
! TTGCAACATTGTAGCTTGTG+AGG - chr8.3:51828025-51828044 MS.gene36133:CDS 40.0%
!! GTAGAAGAAGCACTCTCATT+GGG - chr8.3:51827645-51827664 MS.gene36133:CDS 40.0%
!! TGTAGAAGAAGCACTCTCAT+TGG - chr8.3:51827644-51827663 MS.gene36133:CDS 40.0%
CAAGAACTGTCTCTTGGTCA+TGG + chr8.3:51826786-51826805 None:intergenic 45.0%
CAGGGCAAAACCTTCTGATA+GGG + chr8.3:51826732-51826751 None:intergenic 45.0%
GAGCAAGACACAAACCACAA+TGG - chr8.3:51827870-51827889 MS.gene36133:CDS 45.0%
GATCTTGCTCCATTCTTGCA+GGG + chr8.3:51826750-51826769 None:intergenic 45.0%
GCGAGGTTCCTTATAAGTGA+AGG + chr8.3:51827531-51827550 None:intergenic 45.0%
GTAAGAAGAGAAGGAGTTGC+CGG + chr8.3:51827702-51827721 None:intergenic 45.0%
TATGAACCAAGGCCTTGACT+TGG - chr8.3:51827119-51827138 MS.gene36133:CDS 45.0%
TCTTCATCCTCCATTGTGGT+TGG + chr8.3:51827000-51827019 None:intergenic 45.0%
TGATCTTGCTCCATTCTTGC+AGG + chr8.3:51826751-51826770 None:intergenic 45.0%
TGGTTGCAGAGACAACAATC+AGG - chr8.3:51827173-51827192 MS.gene36133:CDS 45.0%
TTATAAGGAACCTCGCTTCG+AGG - chr8.3:51827535-51827554 MS.gene36133:CDS 45.0%
TTGTGAGGGTCCTGATAGAA+TGG - chr8.3:51828040-51828059 MS.gene36133:CDS 45.0%
! AAGGTTTTGCCCTGCAAGAA+TGG - chr8.3:51826738-51826757 MS.gene36133:CDS 45.0%
! AGGGTTGCCTTTTACTTGGT+TGG - chr8.3:51828201-51828220 MS.gene36133:CDS 45.0%
! TTGCCACAGCATTGAAGTGT+AGG - chr8.3:51827271-51827290 MS.gene36133:CDS 45.0%
!! GCTTGACTTGTACACTTGAG+TGG + chr8.3:51828128-51828147 None:intergenic 45.0%
!! TTGGCTTTGTCAAGTCCTTC+AGG - chr8.3:51827219-51827238 MS.gene36133:CDS 45.0%
CAATGCTGTGGCAAAGCAGT+AGG + chr8.3:51827265-51827284 None:intergenic 50.0%
CCACAATCACAAGCCAGTCT+CGG - chr8.3:51827096-51827115 MS.gene36133:CDS 50.0%
CCTCCAAAGCTCAGGATAAC+AGG - chr8.3:51827567-51827586 MS.gene36133:CDS 50.0%
GCAGGGCAAAACCTTCTGAT+AGG + chr8.3:51826733-51826752 None:intergenic 50.0%
GTAGCGTATGGACCAAGTCA+AGG + chr8.3:51827134-51827153 None:intergenic 50.0%
TATGGACCAAGTCAAGGCCT+TGG + chr8.3:51827128-51827147 None:intergenic 50.0%
! CCTGTTATCCTGAGCTTTGG+AGG + chr8.3:51827570-51827589 None:intergenic 50.0%
!! TGACTTGGTCCATACGCTAC+TGG - chr8.3:51827134-51827153 MS.gene36133:CDS 50.0%
GATGGCTTCTGCTTGGCAAG+TGG - chr8.3:51828241-51828260 MS.gene36133:CDS 55.0%
GCCAGTCTCGGTATGAACCA+AGG - chr8.3:51827108-51827127 MS.gene36133:CDS 55.0%
GCCTTGGTTCATACCGAGAC+TGG + chr8.3:51827112-51827131 None:intergenic 55.0%
!! CCGAGACTGGCTTGTGATTG+TGG + chr8.3:51827099-51827118 None:intergenic 55.0%
AACCTCGCTTCGAGGCCTGA+GGG - chr8.3:51827543-51827562 MS.gene36133:CDS 60.0%
CGGCACAAGCCAGTAGCGTA+TGG + chr8.3:51827146-51827165 None:intergenic 60.0%
GTCTCTGCAACCAACGGCCT+CGG + chr8.3:51827166-51827185 None:intergenic 60.0%
!! TACGCTACTGGCTTGTGCCG+AGG - chr8.3:51827146-51827165 MS.gene36133:CDS 60.0%
ACCTCGCTTCGAGGCCTGAG+GGG - chr8.3:51827544-51827563 MS.gene36133:CDS 65.0%
CTGAGGGGCCTCCAAAGCTC+AGG - chr8.3:51827559-51827578 MS.gene36133:CDS 65.0%
GAACCTCGCTTCGAGGCCTG+AGG - chr8.3:51827542-51827561 MS.gene36133:CDS 65.0%
!! CTGAGCTTTGGAGGCCCCTC+AGG + chr8.3:51827561-51827580 None:intergenic 65.0%
! CTGGCTTGTGCCGAGGCCGT+TGG - chr8.3:51827153-51827172 MS.gene36133:CDS 70.0%
GCCCCTCAGGCCTCGAAGCG+AGG + chr8.3:51827548-51827567 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 51826670 51828295 51826670 ID=MS.gene36133
chr8.3 mRNA 51826670 51828295 51826670 ID=MS.gene36133.t1;Parent=MS.gene36133
chr8.3 exon 51826670 51828295 51826670 ID=MS.gene36133.t1.exon1;Parent=MS.gene36133.t1
chr8.3 CDS 51826670 51828295 51826670 ID=cds.MS.gene36133.t1;Parent=MS.gene36133.t1
Gene Sequence

>MS.gene36133

ATGGAAGACTTAAACATGTGGCTTTCCTTCTATATGAATGAAAGTGTCAACCTTGATTTCCCTATCAGAAGGTTTTGCCCTGCAAGAATGGAGCAAGATCAAGAAGGAGATTGCCATGACCAAGAGACAGTTCTTGATGATGTTGAATTGTGTACTTTTGATACTTCAACCAGTCCTTGTTTATTACCTTCTTCAGAAGTTGATGACTTTGTTGATAGCTTCATCAACATGGATCAATATGAAGATCAAGAGTCTCAAGAGAAGAATCATCAAAGTCAAAGTTTTGATCATCACTTTTTGGTGAATGATGATGTCTTTGGATATGATCCAACCACAATGGAGGATGAAGAATTTGAGATCTGCGAAAATACAACAATCACAAATGAAGACGAAGAAGTTGAAATGAACGGCAATTTTGGTGCTATTCCACAATCACAAGCCAGTCTCGGTATGAACCAAGGCCTTGACTTGGTCCATACGCTACTGGCTTGTGCCGAGGCCGTTGGTTGCAGAGACAACAATCAGGCGAATTTGTTACTTGGTAAAATTTTGGCTTTGTCAAGTCCTTCAGGAGATTCGTTACAAAGAGTCTCCTACTGCTTTGCCACAGCATTGAAGTGTAGGTTATCACTTTATCCACATAATGTATTTTCAAATAGTACACTCACAACAAGTACTTCTAATGATGTTTCTTTGATTACTAGGGAGAATAAGTTGGAAGCTTTTCAACTTCTTTACCAAACAACACCTTACATTACTTTTGGTTTCATGGCTGCAAATGAAGCTATATGCCAAGGATCAAAAGGAAAAAGCTCAATACATATAATTGATTTAGGAATGGAGCATACTCTTCAATGGCCTTCACTTATAAGGAACCTCGCTTCGAGGCCTGAGGGGCCTCCAAAGCTCAGGATAACAGGATTCAGTACGAGTGAAGAAAACAACGCAAAACTTCGTGCTAGCATGTATTTGCTTGTAGAAGAAGCACTCTCATTGGGAATAGTCCTTGAATTTCGCATAATATCAGAGCCGGCAACTCCTTCTCTTCTTACAATCGAAAACCTCAACTTGAGAGAAGGTGAATCTTTATTTGTTAATAGCATCTTGAAATTGCACAAATATGTGAAAGAAAGTAGAGGCTATCTAAAATCAATTCTTCAGTCAATCAAGAAGCTAAGTCCAATTGCACTCACAGTTGTAGAGCAAGACACAAACCACAATGGACCTTTCTTTCTTGGTAGGTTTCTAGAATCACTTCACTATTACTCTGCAATATTTGATTCTCTTGAGGCAAGTATGCCAAGAAATAGTCCAATTAGGATGAAAATAGAGAGAGTACATTTTGCAGAAGAAATTTGCAACATTGTAGCTTGTGAGGGTCCTGATAGAATGGAGAGACATGAAAGAGTAGATCAATGGAGAAGACAATTAGGAAGAGCAGGATTTCAACTAATGCCACTCAAGTGTACAAGTCAAGCTAGAATGATGCTTTCTGTTTATGATTGTGATGGATATACTTTGTCATGTGAAAAGGGTTGCCTTTTACTTGGTTGGAAAGGAAGGTCTATTATGATGGCTTCTGCTTGGCAAGTGGCAAGTTTGTCATATACTGATAGAGCCTCTTAA
Protein sequence

>MS.gene36133.t1

MEDLNMWLSFYMNESVNLDFPIRRFCPARMEQDQEGDCHDQETVLDDVELCTFDTSTSPCLLPSSEVDDFVDSFINMDQYEDQESQEKNHQSQSFDHHFLVNDDVFGYDPTTMEDEEFEICENTTITNEDEEVEMNGNFGAIPQSQASLGMNQGLDLVHTLLACAEAVGCRDNNQANLLLGKILALSSPSGDSLQRVSYCFATALKCRLSLYPHNVFSNSTLTTSTSNDVSLITRENKLEAFQLLYQTTPYITFGFMAANEAICQGSKGKSSIHIIDLGMEHTLQWPSLIRNLASRPEGPPKLRITGFSTSEENNAKLRASMYLLVEEALSLGIVLEFRIISEPATPSLLTIENLNLREGESLFVNSILKLHKYVKESRGYLKSILQSIKKLSPIALTVVEQDTNHNGPFFLGRFLESLHYYSAIFDSLEASMPRNSPIRMKIERVHFAEEICNIVACEGPDRMERHERVDQWRRQLGRAGFQLMPLKCTSQARMMLSVYDCDGYTLSCEKGCLLLGWKGRSIMMASAWQVASLSYTDRAS