Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36534.t1 | XP_013450702.1 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 4.50E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36534.t1 | O23659 | 35.4 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.4e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36534.t1 | A0A072UG20 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.3e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36534.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36534.t1 | MTR_6g004120 | 70.229 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.51e-65 | 195 |
| MS.gene36534.t1 | MTR_1g040600 | 45.695 | 151 | 55 | 4 | 3 | 128 | 5 | 153 | 4.83e-32 | 111 |
| MS.gene36534.t1 | MTR_1g045700 | 50.893 | 112 | 51 | 3 | 23 | 130 | 111 | 222 | 9.43e-30 | 107 |
| MS.gene36534.t1 | MTR_1g046880 | 38.889 | 162 | 62 | 5 | 5 | 129 | 9 | 170 | 1.78e-26 | 97.8 |
| MS.gene36534.t1 | MTR_2g058520 | 44.348 | 115 | 56 | 2 | 23 | 129 | 62 | 176 | 1.65e-25 | 95.5 |
| MS.gene36534.t1 | MTR_1g046720 | 43.966 | 116 | 58 | 3 | 21 | 129 | 2 | 117 | 2.52e-25 | 93.2 |
| MS.gene36534.t1 | MTR_1g046550 | 45.763 | 118 | 58 | 4 | 18 | 129 | 145 | 262 | 3.31e-25 | 96.7 |
| MS.gene36534.t1 | MTR_1g045740 | 41.322 | 121 | 63 | 3 | 22 | 134 | 144 | 264 | 3.61e-24 | 94.4 |
| MS.gene36534.t1 | MTR_1g045720 | 43.966 | 116 | 57 | 3 | 22 | 129 | 144 | 259 | 6.93e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36534.t1 | AT1G78640 | 41.228 | 114 | 58 | 2 | 25 | 129 | 154 | 267 | 1.50e-19 | 82.0 |
| MS.gene36534.t1 | AT2G33720 | 35.398 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.03e-16 | 74.3 |
| MS.gene36534.t1 | AT1G78635 | 37.705 | 122 | 65 | 4 | 26 | 139 | 121 | 239 | 5.51e-15 | 69.3 |
| MS.gene36534.t1 | AT4G02870 | 32.759 | 116 | 62 | 4 | 26 | 131 | 191 | 300 | 3.40e-11 | 59.7 |
Find 22 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATAAGATTGGTCTTTAT+TGG | 0.198682 | 7.1:+7504003 | MS.gene36534:CDS |
| TAGGAATTCGAGCTGAAATA+TGG | 0.273058 | 7.1:+7503868 | MS.gene36534:CDS |
| GGATAAGATTGGTCTTTATT+GGG | 0.344915 | 7.1:+7504004 | MS.gene36534:CDS |
| CTCAAATTCAAGAGATTGTC+TGG | 0.348648 | 7.1:+7503918 | MS.gene36534:CDS |
| GAACAGTAAAATTGAAGAAT+CGG | 0.427975 | 7.1:-7504037 | None:intergenic |
| GACACACTCCTAGAGATAAA+TGG | 0.428020 | 7.1:-7503707 | None:intergenic |
| GTTTGTGACCATTTATCTCT+AGG | 0.448904 | 7.1:+7503699 | MS.gene36534:CDS |
| TTGGGTGGTAATGTTGAGAT+AGG | 0.471139 | 7.1:+7503849 | MS.gene36534:CDS |
| AGGAATTCGAGCTGAAATAT+GGG | 0.489554 | 7.1:+7503869 | MS.gene36534:CDS |
| GTACAAGCAACGATGACTCT+TGG | 0.507283 | 7.1:+7503733 | MS.gene36534:CDS |
| TTATGGCTTATAGCAACAGT+AGG | 0.507490 | 7.1:+7503676 | None:intergenic |
| CTTGAATTTGAGAACGTGCA+TGG | 0.514512 | 7.1:-7503907 | None:intergenic |
| GAGTCATCGTTGCTTGTACA+AGG | 0.516173 | 7.1:-7503729 | None:intergenic |
| TTAAAGCAAGGGGATAAGAT+TGG | 0.521643 | 7.1:+7503993 | MS.gene36534:CDS |
| GTGTTGACAATGAGTGATAC+CGG | 0.535346 | 7.1:+7503768 | MS.gene36534:CDS |
| CCAACAAAAGTCTTCTCATA+CGG | 0.561709 | 7.1:-7503791 | None:intergenic |
| AAAGAAGAGGCTTAAAGCAA+GGG | 0.562567 | 7.1:+7503982 | MS.gene36534:CDS |
| AAAAGAAGAGGCTTAAAGCA+AGG | 0.562720 | 7.1:+7503981 | MS.gene36534:CDS |
| TTTGTGTTGTCTGTGTTGGG+TGG | 0.569410 | 7.1:+7503834 | MS.gene36534:CDS |
| TATGGCTTATAGCAACAGTA+GGG | 0.575545 | 7.1:+7503677 | None:intergenic |
| CAAAAGTCTTCTCATACGGC+CGG | 0.580656 | 7.1:-7503787 | None:intergenic |
| AAGAAGAGGCTTAAAGCAAG+GGG | 0.637026 | 7.1:+7503983 | MS.gene36534:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTGGAATAATAAAAT+TGG | - | chr7.1:7504069-7504088 | None:intergenic | 10.0% |
| ! | GAACAGTAAAATTGAAGAAT+CGG | - | chr7.1:7504040-7504059 | None:intergenic | 25.0% |
| !! | ACTTTTGTTGGGTAAAAAAT+TGG | + | chr7.1:7503803-7503822 | MS.gene36534:CDS | 25.0% |
| GACAAGTTTGTAAAAAGAAG+AGG | + | chr7.1:7503969-7503988 | MS.gene36534:CDS | 30.0% | |
| ! | ATATATTTTCACGGATGGTT+GGG | + | chr7.1:7503944-7503963 | MS.gene36534:CDS | 30.0% |
| ! | CTTCAATTTTACTGTTCTTG+AGG | + | chr7.1:7504043-7504062 | MS.gene36534:CDS | 30.0% |
| ! | GTCTGGATCATATATTTTCA+CGG | + | chr7.1:7503935-7503954 | MS.gene36534:CDS | 30.0% |
| !! | GGATAAGATTGGTCTTTATT+GGG | + | chr7.1:7504004-7504023 | MS.gene36534:CDS | 30.0% |
| AAAAGAAGAGGCTTAAAGCA+AGG | + | chr7.1:7503981-7504000 | MS.gene36534:CDS | 35.0% | |
| AAAGAAGAGGCTTAAAGCAA+GGG | + | chr7.1:7503982-7504001 | MS.gene36534:CDS | 35.0% | |
| AGGAATTCGAGCTGAAATAT+GGG | + | chr7.1:7503869-7503888 | MS.gene36534:CDS | 35.0% | |
| CCAACAAAAGTCTTCTCATA+CGG | - | chr7.1:7503794-7503813 | None:intergenic | 35.0% | |
| CTCAAATTCAAGAGATTGTC+TGG | + | chr7.1:7503918-7503937 | MS.gene36534:CDS | 35.0% | |
| GTTTGTGACCATTTATCTCT+AGG | + | chr7.1:7503699-7503718 | MS.gene36534:CDS | 35.0% | |
| TAGGAATTCGAGCTGAAATA+TGG | + | chr7.1:7503868-7503887 | MS.gene36534:CDS | 35.0% | |
| TTAAAGCAAGGGGATAAGAT+TGG | + | chr7.1:7503993-7504012 | MS.gene36534:CDS | 35.0% | |
| ! | CATATATTTTCACGGATGGT+TGG | + | chr7.1:7503943-7503962 | MS.gene36534:CDS | 35.0% |
| ! | CGTATGAGAAGACTTTTGTT+GGG | + | chr7.1:7503792-7503811 | MS.gene36534:CDS | 35.0% |
| ! | GATTTTGTGTTGTCTGTGTT+GGG | + | chr7.1:7503831-7503850 | MS.gene36534:CDS | 35.0% |
| ! | GGATCATATATTTTCACGGA+TGG | + | chr7.1:7503939-7503958 | MS.gene36534:CDS | 35.0% |
| !! | GGGATAAGATTGGTCTTTAT+TGG | + | chr7.1:7504003-7504022 | MS.gene36534:CDS | 35.0% |
| AAGAAGAGGCTTAAAGCAAG+GGG | + | chr7.1:7503983-7504002 | MS.gene36534:CDS | 40.0% | |
| CTTGAATTTGAGAACGTGCA+TGG | - | chr7.1:7503910-7503929 | None:intergenic | 40.0% | |
| GACACACTCCTAGAGATAAA+TGG | - | chr7.1:7503710-7503729 | None:intergenic | 40.0% | |
| GTTGGGTAAAAAATTGGCTG+AGG | + | chr7.1:7503809-7503828 | MS.gene36534:CDS | 40.0% | |
| ! | CCGTATGAGAAGACTTTTGT+TGG | + | chr7.1:7503791-7503810 | MS.gene36534:CDS | 40.0% |
| ! | GGATTTTGTGTTGTCTGTGT+TGG | + | chr7.1:7503830-7503849 | MS.gene36534:CDS | 40.0% |
| ! | GTGTTGACAATGAGTGATAC+CGG | + | chr7.1:7503768-7503787 | MS.gene36534:CDS | 40.0% |
| ! | TTGGGTGGTAATGTTGAGAT+AGG | + | chr7.1:7503849-7503868 | MS.gene36534:CDS | 40.0% |
| CAAAAGTCTTCTCATACGGC+CGG | - | chr7.1:7503790-7503809 | None:intergenic | 45.0% | |
| GAGTCATCGTTGCTTGTACA+AGG | - | chr7.1:7503732-7503751 | None:intergenic | 45.0% | |
| GTACAAGCAACGATGACTCT+TGG | + | chr7.1:7503733-7503752 | MS.gene36534:CDS | 45.0% | |
| TTTGTGTTGTCTGTGTTGGG+TGG | + | chr7.1:7503834-7503853 | MS.gene36534:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7503678 | 7504097 | 7503678 | ID=MS.gene36534 |
| chr7.1 | mRNA | 7503678 | 7504097 | 7503678 | ID=MS.gene36534.t1;Parent=MS.gene36534 |
| chr7.1 | exon | 7503678 | 7504097 | 7503678 | ID=MS.gene36534.t1.exon1;Parent=MS.gene36534.t1 |
| chr7.1 | CDS | 7503678 | 7504097 | 7503678 | ID=cds.MS.gene36534.t1;Parent=MS.gene36534.t1 |
| Gene Sequence |
| Protein sequence |
