AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36583

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36583.t1 MTR_8g015290 82.353 136 23 1 2 136 241 376 6.83e-73 223
MS.gene36583.t1 MTR_8g015340 82.836 134 23 0 1 134 772 905 3.39e-69 224
MS.gene36583.t1 MTR_2g100450 63.542 96 35 0 5 100 546 641 2.61e-32 120
MS.gene36583.t1 MTR_2g100450 63.542 96 35 0 5 100 751 846 4.69e-32 120
MS.gene36583.t1 MTR_8g015010 44.094 127 66 2 8 133 795 917 3.74e-31 117
MS.gene36583.t1 MTR_8g015100 41.406 128 70 2 7 133 442 565 3.22e-30 114
MS.gene36583.t1 MTR_8g015100 41.732 127 69 2 8 133 660 782 4.89e-30 114
MS.gene36583.t1 MTR_8g028115 40.800 125 69 2 7 130 101 221 9.77e-29 105
MS.gene36583.t1 MTR_8g015040 45.536 112 60 1 7 117 796 907 3.78e-28 109
MS.gene36583.t1 MTR_8g015150 47.115 104 54 1 8 110 495 598 1.56e-27 107
MS.gene36583.t1 MTR_8g015150 45.794 107 57 1 8 113 740 846 2.18e-27 107
MS.gene36583.t1 MTR_8g014790 44.144 111 61 1 8 117 747 857 7.11e-27 105
MS.gene36583.t1 MTR_8g028110 42.342 111 63 1 8 117 752 862 8.15e-27 105
MS.gene36583.t1 MTR_8g014700 42.342 111 63 1 8 117 775 885 1.01e-26 105
MS.gene36583.t1 MTR_8g014970 37.879 132 77 2 3 133 506 633 1.04e-26 105
MS.gene36583.t1 MTR_8g014700 42.342 111 63 1 8 117 751 861 1.17e-26 105
MS.gene36583.t1 MTR_8g015190 41.441 111 64 1 8 117 781 891 2.51e-26 103
MS.gene36583.t1 MTR_8g015190 41.441 111 64 1 8 117 771 881 2.96e-26 103
MS.gene36583.t1 MTR_8g014930 40.625 128 71 2 7 133 744 867 3.28e-26 103
MS.gene36583.t1 MTR_8g014930 40.625 128 71 2 7 133 710 833 4.20e-26 103
MS.gene36583.t1 MTR_8g014760 40.541 111 65 1 8 117 742 852 6.90e-26 102
MS.gene36583.t1 MTR_8g014740 38.095 126 70 2 7 130 107 226 1.44e-24 95.1
MS.gene36583.t1 MTR_8g014690 38.583 127 73 2 2 127 742 864 2.12e-24 98.6
MS.gene36583.t1 MTR_5g058090 47.423 97 50 1 6 101 764 860 1.02e-23 96.7
MS.gene36583.t1 MTR_8g014880 42.697 89 51 0 29 117 11 99 1.06e-22 87.0
MS.gene36583.t1 MTR_6g090615 42.857 98 56 0 6 103 761 858 5.35e-21 89.0
MS.gene36583.t1 MTR_8g461120 39.394 99 59 1 7 104 758 856 5.20e-20 85.9
MS.gene36583.t1 MTR_8g461120 39.394 99 59 1 7 104 733 831 5.88e-20 85.9
MS.gene36583.t1 MTR_8g015170 37.838 111 68 1 8 117 298 408 8.09e-20 85.1
MS.gene36583.t1 MTR_7g061220 37.705 122 69 2 2 116 273 394 5.17e-19 83.2
MS.gene36583.t1 MTR_7g100630 37.705 122 69 2 2 116 779 900 5.82e-19 82.8
MS.gene36583.t1 MTR_1g066950 36.937 111 67 1 2 109 773 883 1.10e-18 82.0
MS.gene36583.t1 MTR_8g014780 35.417 96 58 1 35 130 35 126 4.50e-18 75.9
MS.gene36583.t1 MTR_8g015200 36.634 101 63 1 8 107 722 822 1.72e-17 78.6
MS.gene36583.t1 MTR_3g086120 40.860 93 53 1 3 93 783 875 6.48e-16 74.3
MS.gene36583.t1 MTR_4g105370 43.878 98 51 3 5 98 952 1049 6.24e-15 71.2
MS.gene36583.t1 MTR_3g060880 39.048 105 60 3 6 106 957 1061 1.13e-14 70.9
MS.gene36583.t1 MTR_7g009320 39.000 100 58 3 1 97 305 404 3.12e-14 69.3
MS.gene36583.t1 MTR_5g047070 35.780 109 65 1 5 108 450 558 5.53e-14 68.6
MS.gene36583.t1 MTR_7g073290 38.776 98 57 2 5 99 847 944 8.13e-14 68.2
MS.gene36583.t1 MTR_5g047120 35.780 109 65 1 5 108 691 799 9.03e-14 68.2
MS.gene36583.t1 MTR_6g088510 34.746 118 73 2 3 116 272 389 1.09e-13 67.8
MS.gene36583.t1 MTR_7g074610 39.000 100 58 3 1 97 270 369 1.42e-13 67.4
MS.gene36583.t1 MTR_3g462840 37.624 101 59 3 1 97 247 347 1.50e-13 67.4
MS.gene36583.t1 MTR_8g469230 39.000 100 58 3 1 97 314 413 1.62e-13 67.0
MS.gene36583.t1 MTR_4g129010 34.375 96 61 1 4 97 219 314 1.81e-13 67.0
MS.gene36583.t1 MTR_8g008820 39.048 105 57 3 3 102 261 363 2.16e-13 66.6
MS.gene36583.t1 MTR_6g011570 38.614 101 58 3 1 97 250 350 2.31e-13 66.6
MS.gene36583.t1 MTR_8g074920 33.962 106 65 2 3 103 255 360 2.55e-13 66.6
MS.gene36583.t1 MTR_1g015020 35.644 101 61 2 1 97 261 361 3.35e-13 66.2
MS.gene36583.t1 MTR_7g057170 40.385 104 59 3 3 104 792 894 5.23e-13 65.9
MS.gene36583.t1 MTR_7g018890 38.144 97 54 3 3 93 569 665 5.68e-13 65.9
MS.gene36583.t1 MTR_4g063940 29.167 144 96 4 1 141 277 417 8.05e-13 65.1
MS.gene36583.t1 MTR_6g088610 34.951 103 57 3 3 97 469 569 8.78e-13 65.1
MS.gene36583.t1 MTR_0194s0030 33.645 107 66 2 3 104 505 611 1.07e-12 65.1
MS.gene36583.t1 MTR_1g102190 36.364 99 61 1 5 101 225 323 1.89e-12 63.9
MS.gene36583.t1 MTR_2g098910 36.735 98 62 0 4 101 703 800 2.02e-12 64.3
MS.gene36583.t1 MTR_5g030920 33.010 103 67 2 3 103 750 852 3.58e-12 63.5
MS.gene36583.t1 MTR_5g030920 33.010 103 67 2 3 103 773 875 3.84e-12 63.5
MS.gene36583.t1 MTR_5g013610 34.653 101 63 2 3 100 248 348 4.75e-12 62.8
MS.gene36583.t1 MTR_1g100110 33.333 108 70 1 4 109 662 769 4.95e-12 63.2
MS.gene36583.t1 MTR_2g041960 38.000 100 59 3 1 97 258 357 5.05e-12 62.8
MS.gene36583.t1 MTR_6g011330 40.000 100 57 3 1 97 315 414 5.27e-12 62.8
MS.gene36583.t1 MTR_5g037410 40.336 119 60 4 3 116 514 626 5.61e-12 62.8
MS.gene36583.t1 MTR_2g090710 33.654 104 68 1 5 108 873 975 6.20e-12 62.8
MS.gene36583.t1 MTR_8g070880 37.500 104 57 2 2 100 803 903 6.74e-12 62.8
MS.gene36583.t1 MTR_2g074840 34.694 98 62 1 2 97 838 935 1.64e-11 61.6
MS.gene36583.t1 MTR_4g109010 29.524 105 72 1 3 105 710 814 1.81e-11 61.6
MS.gene36583.t1 MTR_8g088410 33.000 100 64 2 1 97 252 351 1.96e-11 61.2
MS.gene36583.t1 MTR_2g073630 37.234 94 57 1 6 97 807 900 2.35e-11 61.2
MS.gene36583.t1 MTR_5g026370 36.735 98 59 2 3 97 272 369 2.38e-11 60.8
MS.gene36583.t1 MTR_8g070910 36.190 105 59 2 1 100 449 550 2.65e-11 60.8
MS.gene36583.t1 MTR_2g073540 36.170 94 58 1 6 97 605 698 2.67e-11 60.8
MS.gene36583.t1 MTR_2g073520 36.170 94 58 1 6 97 711 804 2.80e-11 60.8
MS.gene36583.t1 MTR_4g028090 34.021 97 61 2 6 99 760 856 5.31e-11 60.1
MS.gene36583.t1 MTR_4g052290 36.752 117 69 4 5 117 706 821 7.37e-11 59.7
MS.gene36583.t1 MTR_4g067230 36.000 100 62 1 4 101 698 797 8.80e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36583.t1 AT1G51800 53.030 132 60 2 3 133 761 891 1.85e-36 133
MS.gene36583.t1 AT1G51880 59.048 105 43 0 3 107 741 845 2.61e-36 132
MS.gene36583.t1 AT2G28960 49.630 135 60 2 3 134 617 746 2.68e-36 132
MS.gene36583.t1 AT1G51880 59.048 105 43 0 3 107 759 863 2.70e-36 132
MS.gene36583.t1 AT1G51880 59.048 105 43 0 3 107 767 871 2.70e-36 132
MS.gene36583.t1 AT4G20450 53.030 132 56 1 3 134 767 892 3.95e-36 132
MS.gene36583.t1 AT2G28960 49.630 135 60 2 3 134 749 878 4.17e-36 132
MS.gene36583.t1 AT1G51910 58.095 105 44 0 3 107 746 850 7.16e-36 131
MS.gene36583.t1 AT1G51870 59.048 105 43 0 3 107 758 862 1.20e-35 130
MS.gene36583.t1 AT1G51870 59.048 105 43 0 3 107 706 810 1.26e-35 130
MS.gene36583.t1 AT1G51890 58.095 105 44 0 3 107 473 577 1.59e-35 129
MS.gene36583.t1 AT1G51860 51.145 131 62 2 3 133 759 887 2.32e-35 130
MS.gene36583.t1 AT1G51860 51.145 131 62 2 3 133 615 743 2.42e-35 129
MS.gene36583.t1 AT1G51890 58.095 105 44 0 3 107 767 871 3.14e-35 129
MS.gene36583.t1 AT1G51890 58.095 105 44 0 3 107 681 785 3.14e-35 129
MS.gene36583.t1 AT1G51890 58.095 105 44 0 3 107 745 849 3.37e-35 129
MS.gene36583.t1 AT1G51850 51.145 131 62 2 3 133 754 882 1.55e-34 127
MS.gene36583.t1 AT1G51830 54.198 131 58 2 3 133 562 690 1.55e-34 127
MS.gene36583.t1 AT2G04300 49.254 134 65 2 3 134 717 849 1.58e-34 127
MS.gene36583.t1 AT2G04300 49.254 134 65 2 3 134 758 890 1.59e-34 127
MS.gene36583.t1 AT1G51830 54.198 131 58 2 3 133 569 697 1.69e-34 127
MS.gene36583.t1 AT1G51850 51.145 131 62 2 3 133 734 862 1.87e-34 127
MS.gene36583.t1 AT2G28990 53.704 108 50 0 3 110 753 860 5.94e-34 125
MS.gene36583.t1 AT2G28990 53.704 108 50 0 3 110 753 860 5.94e-34 125
MS.gene36583.t1 AT2G29000 49.242 132 65 2 3 134 741 870 1.74e-33 124
MS.gene36583.t1 AT1G51820 50.382 131 63 2 3 133 492 620 2.29e-33 124
MS.gene36583.t1 AT1G51820 50.382 131 63 2 3 133 754 882 4.12e-33 123
MS.gene36583.t1 AT1G51790 60.000 105 42 0 3 107 803 907 8.41e-33 122
MS.gene36583.t1 AT1G51790 60.000 105 42 0 3 107 751 855 8.76e-33 122
MS.gene36583.t1 AT1G51790 60.000 105 42 0 3 107 751 855 8.76e-33 122
MS.gene36583.t1 AT1G51810 48.855 131 52 1 3 133 626 741 8.84e-33 122
MS.gene36583.t1 AT3G21340 48.889 135 61 3 3 134 768 897 1.32e-32 122
MS.gene36583.t1 AT1G07560 56.731 104 45 0 3 106 743 846 1.99e-32 121
MS.gene36583.t1 AT1G49100 48.120 133 63 2 3 133 757 885 3.75e-32 120
MS.gene36583.t1 AT4G20450 51.667 120 52 1 15 134 764 877 1.54e-31 119
MS.gene36583.t1 AT2G14440 55.670 97 43 0 4 100 740 836 4.63e-31 117
MS.gene36583.t1 AT5G59670 53.465 101 47 0 3 103 740 840 5.25e-31 117
MS.gene36583.t1 AT5G59670 53.465 101 47 0 3 103 740 840 5.25e-31 117
MS.gene36583.t1 AT1G51805 47.328 131 67 2 3 133 753 881 5.91e-31 117
MS.gene36583.t1 AT1G51805 47.328 131 67 2 3 133 729 857 6.00e-31 117
MS.gene36583.t1 AT5G59680 50.909 110 54 0 3 112 756 865 1.28e-30 116
MS.gene36583.t1 AT2G14510 52.525 99 47 0 2 100 745 843 2.04e-30 115
MS.gene36583.t1 AT2G14510 52.525 99 47 0 2 100 737 835 2.16e-30 115
MS.gene36583.t1 AT2G28970 50.943 106 52 0 3 108 620 725 5.06e-30 114
MS.gene36583.t1 AT2G28970 50.943 106 52 0 3 108 655 760 5.07e-30 114
MS.gene36583.t1 AT3G46355 51.485 101 49 0 3 103 160 260 1.01e-29 110
MS.gene36583.t1 AT5G59650 45.455 132 68 1 3 134 763 890 1.57e-29 113
MS.gene36583.t1 AT5G59650 46.154 130 66 1 5 134 757 882 1.68e-29 113
MS.gene36583.t1 AT5G59660 48.333 120 59 1 3 122 639 755 9.99e-29 110
MS.gene36583.t1 AT5G59660 48.333 120 59 1 3 122 661 777 1.23e-28 110
MS.gene36583.t1 AT5G59660 48.333 120 59 1 3 122 664 780 1.33e-28 110
MS.gene36583.t1 AT4G29990 44.361 133 67 2 3 134 748 874 1.51e-28 110
MS.gene36583.t1 AT3G45920 45.714 105 57 0 2 106 56 160 4.51e-28 103
MS.gene36583.t1 AT2G19210 46.281 121 60 2 2 119 749 867 8.54e-28 108
MS.gene36583.t1 AT3G46420 50.495 101 50 0 3 103 707 807 1.12e-27 107
MS.gene36583.t1 AT3G46410 47.525 101 53 0 3 103 160 260 1.23e-27 104
MS.gene36583.t1 AT4G29180 50.476 105 50 1 5 107 757 861 2.01e-27 107
MS.gene36583.t1 AT4G29180 50.476 105 50 1 5 107 755 859 2.09e-27 107
MS.gene36583.t1 AT4G29180 50.476 105 50 1 5 107 754 858 2.11e-27 107
MS.gene36583.t1 AT2G19190 44.361 133 67 2 3 134 748 874 3.06e-27 106
MS.gene36583.t1 AT3G46370 45.833 120 64 1 5 124 665 783 4.95e-27 106
MS.gene36583.t1 AT3G46370 45.833 120 64 1 5 124 665 783 4.95e-27 106
MS.gene36583.t1 AT3G46400 45.802 131 69 2 3 133 752 880 1.21e-26 105
MS.gene36583.t1 AT1G51620 45.536 112 60 1 3 114 148 258 2.72e-26 100
MS.gene36583.t1 AT1G07550 45.736 129 67 2 6 134 737 862 3.37e-26 103
MS.gene36583.t1 AT3G46340 47.170 106 56 0 3 108 762 867 3.79e-26 103
MS.gene36583.t1 AT5G16900 48.113 106 55 0 5 110 565 670 5.04e-26 103
MS.gene36583.t1 AT3G46330 45.714 105 57 0 2 106 718 822 5.50e-26 103
MS.gene36583.t1 AT3G46330 45.714 105 57 0 2 106 742 846 5.69e-26 103
MS.gene36583.t1 AT5G16900 48.113 106 55 0 5 110 751 856 7.59e-26 102
MS.gene36583.t1 AT5G16900 48.113 106 55 0 5 110 751 856 7.59e-26 102
MS.gene36583.t1 AT1G51620 45.536 112 60 1 3 114 222 332 1.47e-25 99.8
MS.gene36583.t1 AT1G05700 47.664 107 54 2 3 107 775 881 4.60e-25 100
MS.gene36583.t1 AT1G05700 47.664 107 54 2 3 107 744 850 4.67e-25 100
MS.gene36583.t1 AT2G19230 44.615 130 67 3 5 132 745 871 3.18e-24 98.2
MS.gene36583.t1 AT2G19230 43.796 137 72 3 5 138 746 880 3.30e-24 98.2
MS.gene36583.t1 AT4G29450 49.038 104 51 1 6 107 756 859 1.76e-23 95.9
MS.gene36583.t1 AT2G37050 38.261 115 67 2 2 112 779 893 2.51e-17 78.2
MS.gene36583.t1 AT2G37050 38.261 115 67 2 2 112 778 892 2.53e-17 78.2
MS.gene36583.t1 AT5G48740 40.187 107 61 2 5 109 785 890 7.39e-15 71.2
MS.gene36583.t1 AT3G28690 34.228 149 76 5 1 127 257 405 1.07e-14 70.5
MS.gene36583.t1 AT3G28690 34.228 149 76 5 1 127 208 356 1.17e-14 70.5
MS.gene36583.t1 AT3G28690 34.228 149 76 5 1 127 308 456 1.28e-14 70.5
MS.gene36583.t1 AT5G56040 38.679 106 61 3 5 106 942 1047 1.48e-14 70.5
MS.gene36583.t1 AT1G52290 35.294 119 70 2 3 114 317 435 2.59e-14 69.7
MS.gene36583.t1 AT1G10620 39.000 100 55 3 3 96 544 643 1.37e-13 67.4
MS.gene36583.t1 AT5G01020 39.000 100 58 3 1 97 248 347 1.39e-13 67.4
MS.gene36583.t1 AT1G67720 32.039 103 68 1 3 103 781 883 1.96e-13 67.0
MS.gene36583.t1 AT3G01300 40.000 100 57 3 1 97 318 417 4.32e-13 65.9
MS.gene36583.t1 AT5G39000 34.815 135 79 3 6 138 701 828 4.68e-13 66.2
MS.gene36583.t1 AT2G23200 35.577 104 65 1 3 104 663 766 5.42e-13 65.9
MS.gene36583.t1 AT2G28590 35.185 108 67 2 2 106 275 382 1.27e-12 64.7
MS.gene36583.t1 AT5G38990 34.074 135 80 3 6 138 708 835 1.64e-12 64.3
MS.gene36583.t1 AT1G70460 37.000 100 57 3 3 96 527 626 1.98e-12 64.3
MS.gene36583.t1 AT1G29730 37.500 96 57 1 5 97 817 912 3.67e-12 63.5
MS.gene36583.t1 AT1G29730 37.500 96 57 1 5 97 849 944 3.79e-12 63.5
MS.gene36583.t1 AT1G23540 33.000 100 61 2 3 96 545 644 4.25e-12 63.2
MS.gene36583.t1 AT5G65530 33.645 107 68 2 8 114 325 428 1.10e-11 62.0
MS.gene36583.t1 AT3G14840 37.895 95 57 1 5 97 850 944 1.24e-11 62.0
MS.gene36583.t1 AT3G59420 34.694 98 64 0 4 101 693 790 1.25e-11 62.0
MS.gene36583.t1 AT1G29720 34.737 95 60 1 5 97 849 943 2.27e-11 61.2
MS.gene36583.t1 AT3G49670 33.333 117 75 2 5 119 869 984 2.54e-11 61.2
MS.gene36583.t1 AT4G28490 34.483 116 67 4 5 113 874 987 4.23e-11 60.5
MS.gene36583.t1 AT1G30570 34.694 98 62 1 5 100 697 794 4.45e-11 60.5
MS.gene36583.t1 AT1G70530 35.000 100 65 0 1 100 496 595 4.89e-11 60.1
MS.gene36583.t1 AT1G60800 34.043 94 59 1 7 97 415 508 5.11e-11 60.1
MS.gene36583.t1 AT3G15890 34.286 105 63 3 3 101 216 320 5.12e-11 60.1
MS.gene36583.t1 AT3G53380 28.846 104 66 1 1 96 548 651 5.68e-11 60.1
MS.gene36583.t1 AT1G07870 36.842 95 57 2 2 93 280 374 6.35e-11 59.7
MS.gene36583.t1 AT3G55550 32.353 102 67 1 3 102 522 623 7.60e-11 59.7
MS.gene36583.t1 AT1G07870 36.842 95 57 2 2 93 280 374 8.02e-11 59.7
MS.gene36583.t1 AT1G60800 34.043 94 59 1 7 97 482 575 8.67e-11 59.3
MS.gene36583.t1 AT2G39360 28.689 122 85 1 1 120 661 782 9.63e-11 59.3
MS.gene36583.t1 AT4G36180 34.314 102 62 2 2 99 1017 1117 9.88e-11 59.3

Find 31 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAAGGTTCTACCTAATT+CGG 0.230389 8.4:+76020781 None:intergenic
AAAAGTTATGTTTACAGTTT+TGG 0.230925 8.4:-76021154 MS.gene36583:CDS
ACTGAACTCGGGCCCGAATT+AGG 0.323312 8.4:-76020794 MS.gene36583:intron
CTCAATCTCACAACTGAACT+CGG 0.402387 8.4:-76020806 MS.gene36583:CDS
TTGAGGGAAACCAACTCAAT+TGG 0.431589 8.4:+76020824 None:intergenic
GAACGAGCTAAAGGAGTGTT+TGG 0.437028 8.4:-76020900 MS.gene36583:CDS
TTCACTACTTCACTCATGAT+TGG 0.447909 8.4:+76020920 None:intergenic
GCATGGAAAGCTGTTGAGAT+AGG 0.461652 8.4:-76020977 MS.gene36583:CDS
GTTAGATCAATGCTTTATGA+TGG 0.461864 8.4:-76021055 MS.gene36583:CDS
TGGCCTATCGGAAGAATTTG+TGG 0.466511 8.4:+76020940 None:intergenic
GCTGCAGAATTAGCCCGAAA+AGG 0.470236 8.4:-76020878 MS.gene36583:CDS
ATAAAGGTTCTACCTAATTC+GGG 0.476466 8.4:+76020782 None:intergenic
CTGCAGAATTAGCCCGAAAA+GGG 0.478543 8.4:-76020877 MS.gene36583:CDS
TGACTTATGTGAGTCTTGTC+AGG 0.481201 8.4:+76021082 None:intergenic
TTCACTCATGATTGGCCTAT+CGG 0.483011 8.4:+76020928 None:intergenic
GCCCGAAAAGGGGCTGGTGG+TGG 0.487052 8.4:-76020866 MS.gene36583:CDS
CGAGTTCAGTTGTGAGATTG+AGG 0.489163 8.4:+76020807 None:intergenic
TTAGATCAATGCTTTATGAT+GGG 0.495603 8.4:-76021054 MS.gene36583:CDS
TGAAGTAGTGAACGAGCTAA+AGG 0.502913 8.4:-76020909 MS.gene36583:CDS
GCAGATGCATGATATCAAAC+AGG 0.503492 8.4:-76021186 None:intergenic
AAATGAAGGTCCAATTGAGT+TGG 0.508594 8.4:-76020834 MS.gene36583:CDS
GAATTAGCCCGAAAAGGGGC+TGG 0.532477 8.4:-76020872 MS.gene36583:CDS
GAGTTCAGTTGTGAGATTGA+GGG 0.550230 8.4:+76020808 None:intergenic
TAGATCAATGCTTTATGATG+GGG 0.586059 8.4:-76021053 MS.gene36583:CDS
TCAATCTCACAACTGAACTC+GGG 0.605365 8.4:-76020805 MS.gene36583:CDS
TGTCCACAAATTCTTCCGAT+AGG 0.607903 8.4:-76020943 MS.gene36583:CDS
CAAGACTCACATAAGTCAGT+GGG 0.631950 8.4:-76021077 MS.gene36583:CDS
TGCAGAATTAGCCCGAAAAG+GGG 0.645278 8.4:-76020876 MS.gene36583:CDS
GAAAGCTGTTGAGATAGGAA+TGG 0.646564 8.4:-76020972 MS.gene36583:CDS
ACAAGACTCACATAAGTCAG+TGG 0.649111 8.4:-76021078 MS.gene36583:CDS
TTAGCCCGAAAAGGGGCTGG+TGG 0.663374 8.4:-76020869 MS.gene36583:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTTTTACTCACTTT+TGG - chr8.4:76021105-76021124 MS.gene36583:CDS 15.0%
!! TTATGAAACAGAAAATGAAA+TGG + chr8.4:76021078-76021097 None:intergenic 20.0%
!!! AAAAGTTATGTTTACAGTTT+TGG - chr8.4:76020643-76020662 MS.gene36583:CDS 20.0%
!!! ATTTTTAGCATCATTCATAA+AGG + chr8.4:76021034-76021053 None:intergenic 20.0%
! TTAGATCAATGCTTTATGAT+GGG - chr8.4:76020743-76020762 MS.gene36583:intron 25.0%
!! ACTGTAAACATAACTTTTCT+CGG + chr8.4:76020641-76020660 None:intergenic 25.0%
!!! ATTTTGATACTAACTATGCA+TGG - chr8.4:76020803-76020822 MS.gene36583:CDS 25.0%
ATAAAGGTTCTACCTAATTC+GGG + chr8.4:76021018-76021037 None:intergenic 30.0%
CATAAAGGTTCTACCTAATT+CGG + chr8.4:76021019-76021038 None:intergenic 30.0%
GGTGGTAAAACTAAAAATGA+AGG - chr8.4:76020949-76020968 MS.gene36583:CDS 30.0%
GTTAGATCAATGCTTTATGA+TGG - chr8.4:76020742-76020761 MS.gene36583:intron 30.0%
TAGATCAATGCTTTATGATG+GGG - chr8.4:76020744-76020763 MS.gene36583:intron 30.0%
AAATGAAGGTCCAATTGAGT+TGG - chr8.4:76020963-76020982 MS.gene36583:CDS 35.0%
TTCACTACTTCACTCATGAT+TGG + chr8.4:76020880-76020899 None:intergenic 35.0%
!! CTTGTCAGGTGTTTTTGTTA+TGG + chr8.4:76020704-76020723 None:intergenic 35.0%
!!! CAGTTTTGGAGTTGTTCTTT+TGG - chr8.4:76020657-76020676 MS.gene36583:CDS 35.0%
ACAAGACTCACATAAGTCAG+TGG - chr8.4:76020719-76020738 MS.gene36583:intron 40.0%
CAAGACTCACATAAGTCAGT+GGG - chr8.4:76020720-76020739 MS.gene36583:intron 40.0%
CTCAATCTCACAACTGAACT+CGG - chr8.4:76020991-76021010 MS.gene36583:CDS 40.0%
GAAAGCTGTTGAGATAGGAA+TGG - chr8.4:76020825-76020844 MS.gene36583:CDS 40.0%
GAGTTCAGTTGTGAGATTGA+GGG + chr8.4:76020992-76021011 None:intergenic 40.0%
TCAATCTCACAACTGAACTC+GGG - chr8.4:76020992-76021011 MS.gene36583:CDS 40.0%
TGAAGTAGTGAACGAGCTAA+AGG - chr8.4:76020888-76020907 MS.gene36583:CDS 40.0%
TGTCCACAAATTCTTCCGAT+AGG - chr8.4:76020854-76020873 MS.gene36583:CDS 40.0%
TTGAGGGAAACCAACTCAAT+TGG + chr8.4:76020976-76020995 None:intergenic 40.0%
! TGACTTATGTGAGTCTTGTC+AGG + chr8.4:76020718-76020737 None:intergenic 40.0%
!! TCAGGTGTTTTTGTTATGGC+AGG + chr8.4:76020700-76020719 None:intergenic 40.0%
!! TTCACTCATGATTGGCCTAT+CGG + chr8.4:76020872-76020891 None:intergenic 40.0%
CGAGTTCAGTTGTGAGATTG+AGG + chr8.4:76020993-76021012 None:intergenic 45.0%
CTGCAGAATTAGCCCGAAAA+GGG - chr8.4:76020920-76020939 MS.gene36583:CDS 45.0%
GCATGGAAAGCTGTTGAGAT+AGG - chr8.4:76020820-76020839 MS.gene36583:CDS 45.0%
TGCAGAATTAGCCCGAAAAG+GGG - chr8.4:76020921-76020940 MS.gene36583:CDS 45.0%
TGGCCTATCGGAAGAATTTG+TGG + chr8.4:76020860-76020879 None:intergenic 45.0%
!! GAACGAGCTAAAGGAGTGTT+TGG - chr8.4:76020897-76020916 MS.gene36583:CDS 45.0%
GCTGCAGAATTAGCCCGAAA+AGG - chr8.4:76020919-76020938 MS.gene36583:CDS 50.0%
ACTGAACTCGGGCCCGAATT+AGG - chr8.4:76021003-76021022 MS.gene36583:CDS 55.0%
GAATTAGCCCGAAAAGGGGC+TGG - chr8.4:76020925-76020944 MS.gene36583:CDS 55.0%
! TACCACCACCAGCCCCTTTT+CGG + chr8.4:76020936-76020955 None:intergenic 55.0%
TTAGCCCGAAAAGGGGCTGG+TGG - chr8.4:76020928-76020947 MS.gene36583:CDS 60.0%
! ACCACCACCAGCCCCTTTTC+GGG + chr8.4:76020935-76020954 None:intergenic 60.0%
! GCCCGAAAAGGGGCTGGTGG+TGG - chr8.4:76020931-76020950 MS.gene36583:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 76020619 76021200 76020619 ID=MS.gene36583
chr8.4 mRNA 76020619 76021200 76020619 ID=MS.gene36583.t1;Parent=MS.gene36583
chr8.4 exon 76020795 76021200 76020795 ID=MS.gene36583.t1.exon1;Parent=MS.gene36583.t1
chr8.4 CDS 76020795 76021200 76020795 ID=cds.MS.gene36583.t1;Parent=MS.gene36583.t1
chr8.4 exon 76020619 76020683 76020619 ID=MS.gene36583.t1.exon2;Parent=MS.gene36583.t1
chr8.4 CDS 76020619 76020683 76020619 ID=cds.MS.gene36583.t1;Parent=MS.gene36583.t1
Gene Sequence

>MS.gene36583

ATGATATCAAACAGGTTAACCGAGAAAAGTTATGTTTACAGTTTTGGAGTTGTTCTTTTGGAGATAATCACTAGTCAACCTGCCATAACAAAAACACCTGACAAGACTCACATAAGTCAGTGGGTTAGATCAATGCTTTATGATGGGGATATAAAGAATATTGTTGACTCAAGATTACTACAAGATTTTGATACTAACTATGCATGGAAAGCTGTTGAGATAGGAATGGCTTGCGTGTCCACAAATTCTTCCGATAGGCCAATCATGAGTGAAGTAGTGAACGAGCTAAAGGAGTGTTTGGCTGCAGAATTAGCCCGAAAAGGGGCTGGTGGTGGTAAAACTAAAAATGAAGGTCCAATTGAGTTGGTTTCCCTCAATCTCACAACTGAACTCGGGCCCGAATTAGGTAGAACCTTTATGAATGATGCTAAAAATAATACTTTTGTTTGTTGTTGTCCATTTCATTTTCTGTTTCATAAGTATCTTATAATATTTTTACTCACTTTTGGTGTAACAGAGTGTGAAGTATTTTGTTGCAGTTCAATATCTATGTTTCTTTTAGTGAGAACTACTGAAGACTAA
Protein sequence

>MS.gene36583.t1

MISNRLTEKSYVYSFGVVLLEIITSQPAITKTPDKTHISQWVRSMLYDGDIKNIVDSRLLQDFDTNYAWKAVEIGMACVSTNSSDRPIMSEVVNELKECLAAELARKGAGGGKTKNEGPIELVSLNLTTELGPELECEVFCCSSISMFLLVRTTED