Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36636.t1 | XP_024630748.1 | 66.9 | 118 | 39 | 0 | 1 | 118 | 1 | 118 | 1.20E-37 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36636.t1 | Q9LKR4 | 34.0 | 103 | 65 | 2 | 37 | 136 | 30 | 132 | 4.6e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36636.t1 | G7I9C3 | 94.6 | 56 | 3 | 0 | 1 | 56 | 1 | 56 | 1.9e-19 | 105.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36636.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36636.t1 | MTR_1g061650 | 79.710 | 69 | 13 | 1 | 1 | 68 | 1 | 69 | 1.31e-31 | 107 |
| MS.gene36636.t1 | MTR_6g025700 | 61.333 | 75 | 29 | 0 | 1 | 75 | 1 | 75 | 1.08e-27 | 98.6 |
| MS.gene36636.t1 | MTR_4g035820 | 51.456 | 103 | 41 | 3 | 21 | 119 | 35 | 132 | 4.74e-23 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36636.t1 | AT5G28530 | 44.118 | 68 | 35 | 2 | 53 | 117 | 46 | 113 | 2.23e-13 | 66.2 |
| MS.gene36636.t1 | AT5G28530 | 44.118 | 68 | 35 | 2 | 53 | 117 | 46 | 113 | 2.27e-13 | 66.2 |
| MS.gene36636.t1 | AT5G28530 | 44.118 | 68 | 35 | 2 | 53 | 117 | 46 | 113 | 2.27e-13 | 66.2 |
| MS.gene36636.t1 | AT5G28530 | 44.118 | 68 | 35 | 2 | 53 | 117 | 46 | 113 | 2.69e-13 | 65.9 |
| MS.gene36636.t1 | AT5G28530 | 44.118 | 68 | 35 | 2 | 53 | 117 | 46 | 113 | 2.96e-13 | 65.9 |
Find 18 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCACATGGTTCATTATT+TGG | 0.125786 | 7.3:-28224746 | None:intergenic |
| AGAAAGCATTGTCCAATAAA+AGG | 0.242503 | 7.3:-28224839 | None:intergenic |
| CTATTTCGTTGTTCTTTAGA+TGG | 0.256140 | 7.3:-28225015 | None:intergenic |
| GATTGAGAACCCACTCCTTT+TGG | 0.334536 | 7.3:-28224900 | None:intergenic |
| CTAAGTGAGGAAGAAGCTTT+CGG | 0.350154 | 7.3:+28224860 | MS.gene36636:CDS |
| GAGAATTATGCCAAAAGGAG+TGG | 0.432756 | 7.3:+28224890 | MS.gene36636:CDS |
| AACGAAATAGACAATCTTCA+AGG | 0.467235 | 7.3:+28225028 | MS.gene36636:CDS |
| TCAAGGTGTGGATGCAAAGC+GGG | 0.486956 | 7.3:+28225045 | MS.gene36636:CDS |
| TGATTTGAGCAACGACGAGC+AGG | 0.506619 | 7.3:+28224787 | MS.gene36636:CDS |
| TTTATGAGAATTATGCCAAA+AGG | 0.510056 | 7.3:+28224885 | MS.gene36636:CDS |
| AGTGGGTTCTCAATCCGAAA+AGG | 0.554777 | 7.3:+28224908 | MS.gene36636:CDS |
| AAAATGGGATGATACATAGG+AGG | 0.572376 | 7.3:+28224945 | MS.gene36636:CDS |
| CTCAATCAATTGTTCTCATG+TGG | 0.580264 | 7.3:+28224459 | MS.gene36636:CDS |
| AAATGGGATGATACATAGGA+GGG | 0.584474 | 7.3:+28224946 | MS.gene36636:CDS |
| TGGACAATGCTTTCTAAGTG+AGG | 0.591427 | 7.3:+28224847 | MS.gene36636:CDS |
| TTCAAGGTGTGGATGCAAAG+CGG | 0.592179 | 7.3:+28225044 | MS.gene36636:CDS |
| AGAATTATGCCAAAAGGAGT+GGG | 0.597719 | 7.3:+28224891 | MS.gene36636:CDS |
| AATAGACAATCTTCAAGGTG+TGG | 0.612896 | 7.3:+28225033 | MS.gene36636:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATGAATTTATTTGTGT+AGG | + | chr7.3:28224687-28224706 | MS.gene36636:intron | 15.0% |
| !!! | AATTCAACAATAATATTTTG+TGG | + | chr7.3:28224621-28224640 | MS.gene36636:intron | 15.0% |
| !! | AATTCGTATATAAGGAATTA+GGG | - | chr7.3:28224605-28224624 | None:intergenic | 20.0% |
| !! | TATTGTTGAATTCGTATATA+AGG | - | chr7.3:28224613-28224632 | None:intergenic | 20.0% |
| !! | TTGAGAACAATATAAGAAAA+AGG | - | chr7.3:28224533-28224552 | None:intergenic | 20.0% |
| !!! | GTCGATTTCTTAAAAAAAAT+GGG | + | chr7.3:28224930-28224949 | MS.gene36636:CDS | 20.0% |
| ! | AAAAAAATGGGATGATACAT+AGG | + | chr7.3:28224942-28224961 | MS.gene36636:CDS | 25.0% |
| ! | ATTCGTATATAAGGAATTAG+GGG | - | chr7.3:28224604-28224623 | None:intergenic | 25.0% |
| ! | GAATTCGTATATAAGGAATT+AGG | - | chr7.3:28224606-28224625 | None:intergenic | 25.0% |
| ! | TGAATTTATTTGTGTAGGTT+AGG | + | chr7.3:28224692-28224711 | MS.gene36636:intron | 25.0% |
| ! | TTATCACATGGTTCATTATT+TGG | - | chr7.3:28224749-28224768 | None:intergenic | 25.0% |
| ! | TTTATGAGAATTATGCCAAA+AGG | + | chr7.3:28224885-28224904 | MS.gene36636:CDS | 25.0% |
| !! | GGTCGATTTCTTAAAAAAAA+TGG | + | chr7.3:28224929-28224948 | MS.gene36636:CDS | 25.0% |
| !! | TCATTTTCTGAATTATCACA+TGG | - | chr7.3:28224761-28224780 | None:intergenic | 25.0% |
| !!! | AACTGTTTTGAACCTTTTAT+TGG | + | chr7.3:28224827-28224846 | MS.gene36636:CDS | 25.0% |
| !!! | TGGATCATGATTTTTTTTTC+TGG | - | chr7.3:28224998-28225017 | None:intergenic | 25.0% |
| !!! | TTTTAAGAAATCGACCTTTT+CGG | - | chr7.3:28224925-28224944 | None:intergenic | 25.0% |
| AACGAAATAGACAATCTTCA+AGG | + | chr7.3:28225028-28225047 | MS.gene36636:CDS | 30.0% | |
| CTATTTCGTTGTTCTTTAGA+TGG | - | chr7.3:28225018-28225037 | None:intergenic | 30.0% | |
| !! | AGAAAGCATTGTCCAATAAA+AGG | - | chr7.3:28224842-28224861 | None:intergenic | 30.0% |
| !!! | TTTTTTCTGGAAATCCTTCT+CGG | - | chr7.3:28224985-28225004 | None:intergenic | 30.0% |
| AAAATGGGATGATACATAGG+AGG | + | chr7.3:28224945-28224964 | MS.gene36636:CDS | 35.0% | |
| AAATGGGATGATACATAGGA+GGG | + | chr7.3:28224946-28224965 | MS.gene36636:CDS | 35.0% | |
| AATAGACAATCTTCAAGGTG+TGG | + | chr7.3:28225033-28225052 | MS.gene36636:CDS | 35.0% | |
| AGAATTATGCCAAAAGGAGT+GGG | + | chr7.3:28224891-28224910 | MS.gene36636:CDS | 35.0% | |
| CTCAATCAATTGTTCTCATG+TGG | + | chr7.3:28224459-28224478 | MS.gene36636:CDS | 35.0% | |
| CTAAGTGAGGAAGAAGCTTT+CGG | + | chr7.3:28224860-28224879 | MS.gene36636:CDS | 40.0% | |
| GAGAATTATGCCAAAAGGAG+TGG | + | chr7.3:28224890-28224909 | MS.gene36636:CDS | 40.0% | |
| TGGACAATGCTTTCTAAGTG+AGG | + | chr7.3:28224847-28224866 | MS.gene36636:CDS | 40.0% | |
| !!! | GATTTTTTCTGTCACCGAGA+AGG | + | chr7.3:28224968-28224987 | MS.gene36636:CDS | 40.0% |
| TTCAAGGTGTGGATGCAAAG+CGG | + | chr7.3:28225044-28225063 | MS.gene36636:CDS | 45.0% | |
| ! | AGTGGGTTCTCAATCCGAAA+AGG | + | chr7.3:28224908-28224927 | MS.gene36636:CDS | 45.0% |
| ! | GATTGAGAACCCACTCCTTT+TGG | - | chr7.3:28224903-28224922 | None:intergenic | 45.0% |
| TCAAGGTGTGGATGCAAAGC+GGG | + | chr7.3:28225045-28225064 | MS.gene36636:CDS | 50.0% | |
| TGATTTGAGCAACGACGAGC+AGG | + | chr7.3:28224787-28224806 | MS.gene36636:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 28224442 | 28225072 | 28224442 | ID=MS.gene36636 |
| chr7.3 | mRNA | 28224442 | 28225072 | 28224442 | ID=MS.gene36636.t1;Parent=MS.gene36636 |
| chr7.3 | exon | 28224442 | 28224494 | 28224442 | ID=MS.gene36636.t1.exon1;Parent=MS.gene36636.t1 |
| chr7.3 | CDS | 28224442 | 28224494 | 28224442 | ID=cds.MS.gene36636.t1;Parent=MS.gene36636.t1 |
| chr7.3 | exon | 28224709 | 28225072 | 28224709 | ID=MS.gene36636.t1.exon2;Parent=MS.gene36636.t1 |
| chr7.3 | CDS | 28224709 | 28225072 | 28224709 | ID=cds.MS.gene36636.t1;Parent=MS.gene36636.t1 |
| Gene Sequence |
| Protein sequence |
