Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36639.t1 | KEH26852.1 | 60.3 | 63 | 25 | 0 | 1 | 63 | 82 | 144 | 8.10E-14 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36639.t1 | A0A072UB72 | 60.3 | 63 | 25 | 0 | 1 | 63 | 82 | 144 | 5.8e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36639.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36639.t1 | MTR_6g477950 | 60.317 | 63 | 25 | 0 | 1 | 63 | 82 | 144 | 1.72e-21 | 85.1 |
| MS.gene36639.t1 | MTR_7g068970 | 64.615 | 65 | 14 | 2 | 1 | 63 | 27 | 84 | 1.04e-19 | 77.4 |
| MS.gene36639.t1 | MTR_6g034285 | 88.889 | 36 | 4 | 0 | 11 | 46 | 365 | 400 | 9.26e-17 | 72.4 |
| MS.gene36639.t1 | MTR_8g069780 | 49.206 | 63 | 32 | 0 | 1 | 63 | 93 | 155 | 8.57e-14 | 62.0 |
| MS.gene36639.t1 | MTR_5g071410 | 48.438 | 64 | 33 | 0 | 1 | 64 | 206 | 269 | 5.74e-13 | 61.6 |
| MS.gene36639.t1 | MTR_7g117585 | 45.312 | 64 | 35 | 0 | 1 | 64 | 83 | 146 | 6.37e-13 | 59.7 |
| MS.gene36639.t1 | MTR_3g117130 | 52.941 | 51 | 24 | 0 | 1 | 51 | 216 | 266 | 8.71e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGTTGGTTCCAGTTTGTT+AGG | 0.284226 | 7.3:+28256887 | MS.gene36639:CDS |
| CGACAAGCTGCTATTCGATT+TGG | 0.289962 | 7.3:+28256930 | MS.gene36639:CDS |
| GACAAGCTGCTATTCGATTT+GGG | 0.344086 | 7.3:+28256931 | MS.gene36639:CDS |
| GCAGCTTGTCGCCCTCTTGT+AGG | 0.397478 | 7.3:-28256918 | None:intergenic |
| TCCTACAATGTTATTTGTTG+AGG | 0.406389 | 7.3:+28256960 | MS.gene36639:CDS |
| GATTGATGTGCTTACCGGTC+AGG | 0.408212 | 7.3:+28256807 | MS.gene36639:CDS |
| TACAATGTTATTTGTTGAGG+TGG | 0.413661 | 7.3:+28256963 | MS.gene36639:CDS |
| CAGTTGATTGATGTGCTTAC+CGG | 0.428124 | 7.3:+28256802 | MS.gene36639:CDS |
| GGTGATTGTCCCTAACAAAC+TGG | 0.445103 | 7.3:-28256897 | None:intergenic |
| AGGTTGGTTCCAGTTTGTTA+GGG | 0.493750 | 7.3:+28256888 | MS.gene36639:CDS |
| TTCAGCCTTGTTACGCTCAA+CGG | 0.503360 | 7.3:-28256851 | None:intergenic |
| ACCTCAACAAATAACATTGT+AGG | 0.513439 | 7.3:-28256961 | None:intergenic |
| TGTTACGCTCAACGGTAATG+AGG | 0.584146 | 7.3:-28256843 | None:intergenic |
| CATTACCGTTGAGCGTAACA+AGG | 0.590910 | 7.3:+28256846 | MS.gene36639:CDS |
| TCAACAAATAACATTGTAGG+AGG | 0.591551 | 7.3:-28256958 | None:intergenic |
| CTGGAACCAACCTTCTCCCA+AGG | 0.599362 | 7.3:-28256878 | None:intergenic |
| GCGGCAATCGTATTCCTGAC+CGG | 0.622702 | 7.3:-28256821 | None:intergenic |
| TAGGGACAATCACCTACAAG+AGG | 0.660602 | 7.3:+28256906 | MS.gene36639:CDS |
| TACGCTCAACGGTAATGAGG+CGG | 0.676660 | 7.3:-28256840 | None:intergenic |
| AGGGACAATCACCTACAAGA+GGG | 0.691250 | 7.3:+28256907 | MS.gene36639:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCTCAACAAATAACATTGT+AGG | - | chr7.3:28256964-28256983 | None:intergenic | 30.0% | |
| TCAACAAATAACATTGTAGG+AGG | - | chr7.3:28256961-28256980 | None:intergenic | 30.0% | |
| ! | TACAATGTTATTTGTTGAGG+TGG | + | chr7.3:28256963-28256982 | MS.gene36639:CDS | 30.0% |
| ! | TCCTACAATGTTATTTGTTG+AGG | + | chr7.3:28256960-28256979 | MS.gene36639:CDS | 30.0% |
| AACAAGGCTGAAAAATCCTT+GGG | + | chr7.3:28256862-28256881 | MS.gene36639:CDS | 35.0% | |
| TAACAAGGCTGAAAAATCCT+TGG | + | chr7.3:28256861-28256880 | MS.gene36639:CDS | 35.0% | |
| AAAAATCCTTGGGAGAAGGT+TGG | + | chr7.3:28256872-28256891 | MS.gene36639:CDS | 40.0% | |
| CAGTTGATTGATGTGCTTAC+CGG | + | chr7.3:28256802-28256821 | MS.gene36639:CDS | 40.0% | |
| GACAAGCTGCTATTCGATTT+GGG | + | chr7.3:28256931-28256950 | MS.gene36639:CDS | 40.0% | |
| ! | AAGGTTGGTTCCAGTTTGTT+AGG | + | chr7.3:28256887-28256906 | MS.gene36639:CDS | 40.0% |
| !! | AGGTTGGTTCCAGTTTGTTA+GGG | + | chr7.3:28256888-28256907 | MS.gene36639:CDS | 40.0% |
| AGGGACAATCACCTACAAGA+GGG | + | chr7.3:28256907-28256926 | MS.gene36639:CDS | 45.0% | |
| CATTACCGTTGAGCGTAACA+AGG | + | chr7.3:28256846-28256865 | MS.gene36639:CDS | 45.0% | |
| CGACAAGCTGCTATTCGATT+TGG | + | chr7.3:28256930-28256949 | MS.gene36639:CDS | 45.0% | |
| GCTGAAAAATCCTTGGGAGA+AGG | + | chr7.3:28256868-28256887 | MS.gene36639:CDS | 45.0% | |
| GGTGATTGTCCCTAACAAAC+TGG | - | chr7.3:28256900-28256919 | None:intergenic | 45.0% | |
| TAGGGACAATCACCTACAAG+AGG | + | chr7.3:28256906-28256925 | MS.gene36639:CDS | 45.0% | |
| TGTTACGCTCAACGGTAATG+AGG | - | chr7.3:28256846-28256865 | None:intergenic | 45.0% | |
| !! | TTCAGCCTTGTTACGCTCAA+CGG | - | chr7.3:28256854-28256873 | None:intergenic | 45.0% |
| GATTGATGTGCTTACCGGTC+AGG | + | chr7.3:28256807-28256826 | MS.gene36639:CDS | 50.0% | |
| TACGCTCAACGGTAATGAGG+CGG | - | chr7.3:28256843-28256862 | None:intergenic | 50.0% | |
| CTGGAACCAACCTTCTCCCA+AGG | - | chr7.3:28256881-28256900 | None:intergenic | 55.0% | |
| GCGGCAATCGTATTCCTGAC+CGG | - | chr7.3:28256824-28256843 | None:intergenic | 55.0% | |
| GCAGCTTGTCGCCCTCTTGT+AGG | - | chr7.3:28256921-28256940 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 28256799 | 28257008 | 28256799 | ID=MS.gene36639 |
| chr7.3 | mRNA | 28256799 | 28257008 | 28256799 | ID=MS.gene36639.t1;Parent=MS.gene36639 |
| chr7.3 | exon | 28256799 | 28257008 | 28256799 | ID=MS.gene36639.t1.exon1;Parent=MS.gene36639.t1 |
| chr7.3 | CDS | 28256799 | 28257008 | 28256799 | ID=cds.MS.gene36639.t1;Parent=MS.gene36639.t1 |
| Gene Sequence |
| Protein sequence |
