AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36689

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36689.t1 MTR_8g015290 83.088 136 22 1 2 136 241 376 1.03e-73 225
MS.gene36689.t1 MTR_8g015340 83.582 134 22 0 1 134 772 905 6.05e-70 226
MS.gene36689.t1 MTR_2g100450 63.542 96 35 0 5 100 546 641 4.39e-32 120
MS.gene36689.t1 MTR_2g100450 63.542 96 35 0 5 100 751 846 7.83e-32 119
MS.gene36689.t1 MTR_8g015010 44.094 127 66 2 8 133 795 917 3.02e-31 118
MS.gene36689.t1 MTR_8g015100 41.732 127 69 2 8 133 443 565 2.42e-30 115
MS.gene36689.t1 MTR_8g015100 41.732 127 69 2 8 133 660 782 3.80e-30 115
MS.gene36689.t1 MTR_8g028115 40.800 125 69 2 7 130 101 221 6.93e-29 106
MS.gene36689.t1 MTR_8g015040 45.946 111 59 1 8 117 797 907 2.67e-28 109
MS.gene36689.t1 MTR_8g015150 47.115 104 54 1 8 110 495 598 1.37e-27 107
MS.gene36689.t1 MTR_8g015150 45.794 107 57 1 8 113 740 846 1.67e-27 107
MS.gene36689.t1 MTR_8g014790 44.144 111 61 1 8 117 747 857 6.04e-27 105
MS.gene36689.t1 MTR_8g028110 42.342 111 63 1 8 117 752 862 6.28e-27 105
MS.gene36689.t1 MTR_8g014700 42.342 111 63 1 8 117 775 885 8.42e-27 105
MS.gene36689.t1 MTR_8g014700 42.342 111 63 1 8 117 751 861 9.89e-27 105
MS.gene36689.t1 MTR_8g014970 37.879 132 77 2 3 133 506 633 1.30e-26 104
MS.gene36689.t1 MTR_8g015190 41.441 111 64 1 8 117 781 891 1.79e-26 104
MS.gene36689.t1 MTR_8g015190 41.441 111 64 1 8 117 771 881 2.01e-26 104
MS.gene36689.t1 MTR_8g014930 40.945 127 70 2 8 133 745 867 2.27e-26 104
MS.gene36689.t1 MTR_8g014930 40.945 127 70 2 8 133 711 833 2.79e-26 103
MS.gene36689.t1 MTR_8g014760 40.541 111 65 1 8 117 742 852 5.21e-26 103
MS.gene36689.t1 MTR_8g014740 38.400 125 69 2 8 130 108 226 8.93e-25 95.5
MS.gene36689.t1 MTR_8g014690 38.583 127 73 2 2 127 742 864 1.51e-24 99.0
MS.gene36689.t1 MTR_5g058090 47.423 97 50 1 6 101 764 860 1.15e-23 96.3
MS.gene36689.t1 MTR_8g014880 42.697 89 51 0 29 117 11 99 8.79e-23 87.4
MS.gene36689.t1 MTR_6g090615 42.857 98 56 0 6 103 761 858 4.41e-21 89.0
MS.gene36689.t1 MTR_8g015170 37.838 111 68 1 8 117 298 408 6.70e-20 85.1
MS.gene36689.t1 MTR_8g461120 39.796 98 58 1 8 104 759 856 1.00e-19 85.1
MS.gene36689.t1 MTR_8g461120 39.796 98 58 1 8 104 734 831 1.20e-19 85.1
MS.gene36689.t1 MTR_7g061220 37.705 122 69 2 2 116 273 394 3.04e-19 83.6
MS.gene36689.t1 MTR_7g100630 37.705 122 69 2 2 116 779 900 4.15e-19 83.6
MS.gene36689.t1 MTR_1g066950 36.937 111 67 1 2 109 773 883 8.07e-19 82.4
MS.gene36689.t1 MTR_8g014780 35.417 96 58 1 35 130 35 126 3.09e-18 76.3
MS.gene36689.t1 MTR_8g015200 36.634 101 63 1 8 107 722 822 1.31e-17 79.0
MS.gene36689.t1 MTR_3g086120 38.384 99 59 1 3 99 783 881 5.56e-16 74.3
MS.gene36689.t1 MTR_4g105370 43.878 98 51 3 5 98 952 1049 4.05e-15 72.0
MS.gene36689.t1 MTR_3g060880 39.048 105 60 3 6 106 957 1061 8.25e-15 71.2
MS.gene36689.t1 MTR_7g009320 39.216 102 55 4 1 97 305 404 4.05e-14 68.9
MS.gene36689.t1 MTR_5g047070 35.780 109 65 1 5 108 450 558 4.35e-14 68.9
MS.gene36689.t1 MTR_6g088510 34.746 118 73 2 3 116 272 389 6.24e-14 68.2
MS.gene36689.t1 MTR_5g047120 35.780 109 65 1 5 108 691 799 7.67e-14 68.2
MS.gene36689.t1 MTR_3g462840 37.624 101 59 3 1 97 247 347 1.32e-13 67.4
MS.gene36689.t1 MTR_4g129010 34.375 96 61 1 4 97 219 314 1.49e-13 67.0
MS.gene36689.t1 MTR_7g073290 38.776 98 57 2 5 99 847 944 1.59e-13 67.4
MS.gene36689.t1 MTR_4g063940 29.861 144 95 4 1 141 277 417 1.83e-13 67.0
MS.gene36689.t1 MTR_6g011570 38.614 101 58 3 1 97 250 350 1.98e-13 67.0
MS.gene36689.t1 MTR_8g074920 33.962 106 65 2 3 103 255 360 2.27e-13 66.6
MS.gene36689.t1 MTR_8g008820 39.048 105 57 3 3 102 261 363 2.52e-13 66.6
MS.gene36689.t1 MTR_7g057170 40.385 104 59 3 3 104 792 894 4.57e-13 65.9
MS.gene36689.t1 MTR_7g074610 38.000 100 59 3 1 97 270 369 6.32e-13 65.5
MS.gene36689.t1 MTR_6g088610 34.951 103 57 3 3 97 469 569 6.58e-13 65.5
MS.gene36689.t1 MTR_7g018890 38.144 97 54 3 3 93 569 665 1.09e-12 65.1
MS.gene36689.t1 MTR_2g096950 35.644 101 62 3 1 98 258 358 1.25e-12 64.7
MS.gene36689.t1 MTR_1g015020 34.653 101 62 2 1 97 261 361 1.34e-12 64.3
MS.gene36689.t1 MTR_2g098910 36.735 98 62 0 4 101 703 800 1.36e-12 64.7
MS.gene36689.t1 MTR_1g102190 36.364 99 61 1 5 101 225 323 1.36e-12 64.3
MS.gene36689.t1 MTR_5g030920 33.010 103 67 2 3 103 750 852 3.04e-12 63.5
MS.gene36689.t1 MTR_5g030920 33.010 103 67 2 3 103 773 875 3.11e-12 63.5
MS.gene36689.t1 MTR_5g037410 40.336 119 60 4 3 116 514 626 3.12e-12 63.5
MS.gene36689.t1 MTR_1g100110 33.333 108 70 1 4 109 662 769 3.47e-12 63.5
MS.gene36689.t1 MTR_0194s0030 32.710 107 67 2 3 104 505 611 4.19e-12 63.2
MS.gene36689.t1 MTR_8g070910 37.143 105 58 2 1 100 449 550 5.92e-12 62.8
MS.gene36689.t1 MTR_5g013610 34.951 103 60 3 3 100 248 348 8.77e-12 62.0
MS.gene36689.t1 MTR_6g011330 40.196 102 54 4 1 97 315 414 1.00e-11 62.0
MS.gene36689.t1 MTR_4g109010 29.524 105 72 1 3 105 710 814 1.03e-11 62.0
MS.gene36689.t1 MTR_2g074840 34.694 98 62 1 2 97 838 935 1.10e-11 62.0
MS.gene36689.t1 MTR_2g090710 33.654 104 68 1 5 108 873 975 1.20e-11 62.0
MS.gene36689.t1 MTR_5g026370 36.735 98 59 2 3 97 272 369 1.70e-11 61.2
MS.gene36689.t1 MTR_2g041960 37.000 100 60 3 1 97 258 357 2.31e-11 60.8
MS.gene36689.t1 MTR_8g070880 36.538 104 58 2 2 100 803 903 3.08e-11 60.8
MS.gene36689.t1 MTR_2g005810 40.206 97 53 3 5 98 866 960 3.57e-11 60.5
MS.gene36689.t1 MTR_2g073630 37.234 94 57 1 6 97 807 900 4.39e-11 60.5
MS.gene36689.t1 MTR_2g073540 36.170 94 58 1 6 97 605 698 5.08e-11 60.1
MS.gene36689.t1 MTR_2g073520 36.170 94 58 1 6 97 711 804 5.33e-11 60.1
MS.gene36689.t1 MTR_6g015805 37.624 101 61 1 5 103 714 814 6.34e-11 59.7
MS.gene36689.t1 MTR_4g067230 36.000 100 62 1 4 101 698 797 6.41e-11 59.7
MS.gene36689.t1 MTR_3g113140 31.068 103 66 2 1 99 1029 1130 7.43e-11 59.7
MS.gene36689.t1 MTR_2g075060 35.106 94 59 2 6 97 841 934 7.77e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36689.t1 AT4G20450 53.788 132 55 1 3 134 767 892 9.14e-37 134
MS.gene36689.t1 AT1G51800 53.030 132 60 2 3 133 761 891 1.35e-36 133
MS.gene36689.t1 AT1G51880 59.048 105 43 0 3 107 759 863 2.50e-36 132
MS.gene36689.t1 AT1G51880 59.048 105 43 0 3 107 741 845 2.59e-36 132
MS.gene36689.t1 AT1G51880 59.048 105 43 0 3 107 767 871 2.60e-36 132
MS.gene36689.t1 AT2G28960 49.630 135 60 2 3 134 617 746 2.63e-36 132
MS.gene36689.t1 AT2G28960 49.630 135 60 2 3 134 749 878 3.93e-36 132
MS.gene36689.t1 AT1G51890 59.048 105 43 0 3 107 473 577 4.70e-36 131
MS.gene36689.t1 AT1G51890 59.048 105 43 0 3 107 767 871 8.73e-36 131
MS.gene36689.t1 AT1G51890 59.048 105 43 0 3 107 681 785 9.43e-36 130
MS.gene36689.t1 AT1G51890 59.048 105 43 0 3 107 745 849 9.86e-36 130
MS.gene36689.t1 AT1G51870 59.048 105 43 0 3 107 758 862 1.15e-35 130
MS.gene36689.t1 AT1G51910 58.095 105 44 0 3 107 746 850 1.28e-35 130
MS.gene36689.t1 AT1G51870 59.048 105 43 0 3 107 706 810 1.33e-35 130
MS.gene36689.t1 AT1G51860 51.145 131 62 2 3 133 759 887 1.93e-35 130
MS.gene36689.t1 AT1G51860 51.145 131 62 2 3 133 615 743 1.98e-35 130
MS.gene36689.t1 AT1G51850 51.145 131 62 2 3 133 754 882 1.29e-34 127
MS.gene36689.t1 AT1G51830 54.198 131 58 2 3 133 569 697 1.35e-34 127
MS.gene36689.t1 AT1G51850 51.145 131 62 2 3 133 734 862 1.37e-34 127
MS.gene36689.t1 AT1G51830 54.198 131 58 2 3 133 562 690 1.51e-34 127
MS.gene36689.t1 AT2G04300 49.254 134 65 2 3 134 717 849 1.56e-34 127
MS.gene36689.t1 AT2G04300 49.254 134 65 2 3 134 758 890 1.76e-34 127
MS.gene36689.t1 AT2G28990 53.704 108 50 0 3 110 753 860 5.14e-34 126
MS.gene36689.t1 AT2G28990 53.704 108 50 0 3 110 753 860 5.14e-34 126
MS.gene36689.t1 AT2G29000 49.242 132 65 2 3 134 741 870 1.58e-33 124
MS.gene36689.t1 AT1G51820 50.382 131 63 2 3 133 492 620 1.82e-33 124
MS.gene36689.t1 AT1G51820 50.382 131 63 2 3 133 754 882 3.39e-33 123
MS.gene36689.t1 AT1G51790 60.000 105 42 0 3 107 803 907 6.17e-33 123
MS.gene36689.t1 AT1G51790 60.000 105 42 0 3 107 751 855 6.62e-33 122
MS.gene36689.t1 AT1G51790 60.000 105 42 0 3 107 751 855 6.62e-33 122
MS.gene36689.t1 AT1G51810 48.855 131 52 1 3 133 626 741 8.75e-33 122
MS.gene36689.t1 AT3G21340 48.889 135 61 3 3 134 768 897 1.34e-32 122
MS.gene36689.t1 AT1G07560 56.731 104 45 0 3 106 743 846 1.50e-32 122
MS.gene36689.t1 AT1G49100 48.120 133 63 2 3 133 757 885 3.21e-32 120
MS.gene36689.t1 AT4G20450 52.500 120 51 1 15 134 764 877 3.53e-32 120
MS.gene36689.t1 AT2G14440 56.701 97 42 0 4 100 740 836 1.12e-31 119
MS.gene36689.t1 AT1G51805 47.328 131 67 2 3 133 753 881 4.25e-31 117
MS.gene36689.t1 AT5G59670 53.465 101 47 0 3 103 740 840 4.32e-31 117
MS.gene36689.t1 AT5G59670 53.465 101 47 0 3 103 740 840 4.32e-31 117
MS.gene36689.t1 AT1G51805 47.328 131 67 2 3 133 729 857 4.58e-31 117
MS.gene36689.t1 AT2G14510 53.535 99 46 0 2 100 737 835 5.20e-31 117
MS.gene36689.t1 AT2G14510 53.535 99 46 0 2 100 745 843 5.20e-31 117
MS.gene36689.t1 AT5G59680 50.909 110 54 0 3 112 756 865 1.02e-30 116
MS.gene36689.t1 AT2G28970 50.943 106 52 0 3 108 620 725 3.94e-30 115
MS.gene36689.t1 AT2G28970 50.943 106 52 0 3 108 655 760 4.06e-30 115
MS.gene36689.t1 AT3G46355 51.485 101 49 0 3 103 160 260 6.97e-30 110
MS.gene36689.t1 AT5G59650 45.455 132 68 1 3 134 763 890 1.15e-29 113
MS.gene36689.t1 AT5G59650 46.154 130 66 1 5 134 757 882 1.23e-29 113
MS.gene36689.t1 AT4G29990 45.113 133 66 2 3 134 748 874 3.24e-29 112
MS.gene36689.t1 AT5G59660 48.333 120 59 1 3 122 639 755 7.92e-29 111
MS.gene36689.t1 AT5G59660 48.333 120 59 1 3 122 664 780 9.46e-29 110
MS.gene36689.t1 AT5G59660 48.333 120 59 1 3 122 661 777 9.55e-29 110
MS.gene36689.t1 AT3G45920 45.714 105 57 0 2 106 56 160 3.96e-28 103
MS.gene36689.t1 AT2G19210 46.281 121 60 2 2 119 749 867 5.58e-28 108
MS.gene36689.t1 AT2G19190 45.113 133 66 2 3 134 748 874 6.03e-28 108
MS.gene36689.t1 AT3G46410 47.525 101 53 0 3 103 160 260 8.15e-28 105
MS.gene36689.t1 AT3G46420 50.495 101 50 0 3 103 707 807 8.25e-28 108
MS.gene36689.t1 AT4G29180 50.476 105 50 1 5 107 757 861 1.46e-27 107
MS.gene36689.t1 AT4G29180 50.476 105 50 1 5 107 755 859 1.55e-27 107
MS.gene36689.t1 AT4G29180 50.476 105 50 1 5 107 754 858 1.59e-27 107
MS.gene36689.t1 AT3G46370 45.833 120 64 1 5 124 665 783 4.00e-27 106
MS.gene36689.t1 AT3G46370 45.833 120 64 1 5 124 665 783 4.00e-27 106
MS.gene36689.t1 AT3G46400 45.802 131 69 2 3 133 752 880 8.14e-27 105
MS.gene36689.t1 AT1G07550 46.512 129 66 2 6 134 737 862 8.44e-27 105
MS.gene36689.t1 AT1G51620 45.536 112 60 1 3 114 148 258 2.55e-26 100
MS.gene36689.t1 AT3G46340 47.170 106 56 0 3 108 762 867 2.78e-26 103
MS.gene36689.t1 AT5G16900 48.113 106 55 0 5 110 565 670 3.49e-26 103
MS.gene36689.t1 AT5G16900 48.113 106 55 0 5 110 751 856 5.46e-26 103
MS.gene36689.t1 AT5G16900 48.113 106 55 0 5 110 751 856 5.46e-26 103
MS.gene36689.t1 AT3G46330 45.714 105 57 0 2 106 742 846 5.97e-26 103
MS.gene36689.t1 AT3G46330 45.714 105 57 0 2 106 718 822 6.01e-26 103
MS.gene36689.t1 AT1G51620 45.536 112 60 1 3 114 222 332 1.18e-25 100
MS.gene36689.t1 AT1G05700 47.664 107 54 2 3 107 744 850 4.04e-25 100
MS.gene36689.t1 AT1G05700 47.664 107 54 2 3 107 775 881 4.42e-25 100
MS.gene36689.t1 AT2G19230 44.275 131 68 3 4 132 745 872 2.35e-24 98.6
MS.gene36689.t1 AT2G19230 44.615 130 67 3 5 132 745 871 2.57e-24 98.2
MS.gene36689.t1 AT4G29450 49.038 104 51 1 6 107 756 859 1.52e-23 96.3
MS.gene36689.t1 AT2G37050 39.130 115 66 2 2 112 778 892 5.90e-18 80.1
MS.gene36689.t1 AT2G37050 39.130 115 66 2 2 112 779 893 6.13e-18 80.1
MS.gene36689.t1 AT5G48740 40.187 107 61 2 5 109 785 890 6.27e-15 71.6
MS.gene36689.t1 AT5G56040 38.679 106 61 3 5 106 942 1047 9.32e-15 70.9
MS.gene36689.t1 AT3G28690 33.557 149 77 5 1 127 257 405 5.56e-14 68.6
MS.gene36689.t1 AT3G28690 33.557 149 77 5 1 127 208 356 6.04e-14 68.2
MS.gene36689.t1 AT3G28690 33.557 149 77 5 1 127 308 456 6.67e-14 68.2
MS.gene36689.t1 AT1G52290 34.454 119 71 2 3 114 317 435 8.64e-14 68.2
MS.gene36689.t1 AT1G67720 32.039 103 68 1 3 103 781 883 1.76e-13 67.4
MS.gene36689.t1 AT5G39000 34.815 135 79 3 6 138 701 828 2.60e-13 66.6
MS.gene36689.t1 AT1G10620 39.000 100 55 3 3 96 544 643 2.74e-13 66.6
MS.gene36689.t1 AT2G23200 35.577 104 65 1 3 104 663 766 4.26e-13 66.2
MS.gene36689.t1 AT5G01020 38.000 100 59 3 1 97 248 347 5.62e-13 65.5
MS.gene36689.t1 AT5G38990 34.074 135 80 3 6 138 708 835 1.21e-12 64.7
MS.gene36689.t1 AT1G70460 37.000 100 57 3 3 96 527 626 1.59e-12 64.3
MS.gene36689.t1 AT3G01300 39.000 100 58 3 1 97 318 417 1.94e-12 64.3
MS.gene36689.t1 AT1G29730 37.500 96 57 1 5 97 817 912 2.89e-12 63.9
MS.gene36689.t1 AT1G29730 37.500 96 57 1 5 97 849 944 3.19e-12 63.5
MS.gene36689.t1 AT1G23540 33.000 100 61 2 3 96 545 644 3.47e-12 63.5
MS.gene36689.t1 AT2G28590 34.259 108 68 2 2 106 275 382 4.86e-12 62.8
MS.gene36689.t1 AT5G65530 33.028 109 70 2 6 114 323 428 7.32e-12 62.4
MS.gene36689.t1 AT3G49670 35.043 117 73 3 5 119 869 984 9.28e-12 62.4
MS.gene36689.t1 AT3G59420 34.694 98 64 0 4 101 693 790 1.03e-11 62.0
MS.gene36689.t1 AT3G14840 37.895 95 57 1 5 97 850 944 1.09e-11 62.0
MS.gene36689.t1 AT1G29720 34.737 95 60 1 5 97 849 943 2.02e-11 61.2
MS.gene36689.t1 AT1G07570 34.653 101 63 2 1 98 136 236 3.19e-11 60.1
MS.gene36689.t1 AT4G28490 34.483 116 67 4 5 113 874 987 3.63e-11 60.5
MS.gene36689.t1 AT1G07570 34.653 101 63 2 1 98 252 352 3.81e-11 60.5
MS.gene36689.t1 AT1G07570 34.653 101 63 2 1 98 252 352 3.81e-11 60.5
MS.gene36689.t1 AT1G07570 34.653 101 63 2 1 98 252 352 3.81e-11 60.5
MS.gene36689.t1 AT1G70530 35.000 100 65 0 1 100 496 595 3.85e-11 60.5
MS.gene36689.t1 AT1G07570 34.653 101 63 2 1 98 263 363 4.27e-11 60.1
MS.gene36689.t1 AT3G55550 32.353 102 67 1 3 102 522 623 4.89e-11 60.1
MS.gene36689.t1 AT1G30570 34.694 98 62 1 5 100 697 794 4.94e-11 60.1
MS.gene36689.t1 AT4G36180 34.314 102 62 2 2 99 1017 1117 6.23e-11 60.1
MS.gene36689.t1 AT2G39360 28.689 122 85 1 1 120 661 782 6.37e-11 60.1
MS.gene36689.t1 AT1G69910 37.500 104 56 3 3 97 517 620 6.89e-11 59.7
MS.gene36689.t1 AT1G60800 34.043 94 59 1 7 97 415 508 8.50e-11 59.3
MS.gene36689.t1 AT3G15890 34.286 105 63 3 3 101 216 320 9.14e-11 59.3

Find 31 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAAGGTTCTACCTAATT+CGG 0.230389 8.2:+77924012 None:intergenic
AAAAGTTATGTTTACAGTTT+TGG 0.230925 8.2:-77924385 MS.gene36689:CDS
GTTAGATCAATGCTTTATAA+TGG 0.316368 8.2:-77924286 MS.gene36689:CDS
ACTGAACTCGGGCCCGAATT+AGG 0.323312 8.2:-77924025 MS.gene36689:intron
TTAGATCAATGCTTTATAAT+GGG 0.325390 8.2:-77924285 MS.gene36689:CDS
CTCAATCTCACAACTGAACT+CGG 0.402387 8.2:-77924037 MS.gene36689:CDS
TTGAGGGAAACCAACTCAAT+TGG 0.431589 8.2:+77924055 None:intergenic
GAACGAGCTAAAGGAGTGTT+TGG 0.437028 8.2:-77924131 MS.gene36689:CDS
TTCACTACTTCACTCATGAT+TGG 0.447909 8.2:+77924151 None:intergenic
GCATGGAAAGCTGTTGAGAT+AGG 0.461652 8.2:-77924208 MS.gene36689:CDS
TGGCCTATCGGAAGAATTTG+TGG 0.466511 8.2:+77924171 None:intergenic
GCTGCAGAATTAGCCCGAAA+AGG 0.470236 8.2:-77924109 MS.gene36689:CDS
ATAAAGGTTCTACCTAATTC+GGG 0.476466 8.2:+77924013 None:intergenic
CTGCAGAATTAGCCCGAAAA+GGG 0.478543 8.2:-77924108 MS.gene36689:CDS
TGACTTATGTGAGTCTTGTC+AGG 0.481201 8.2:+77924313 None:intergenic
TTCACTCATGATTGGCCTAT+CGG 0.483011 8.2:+77924159 None:intergenic
GCCCGAAAAGGGGCTGGTGG+TGG 0.487052 8.2:-77924097 MS.gene36689:CDS
CGAGTTCAGTTGTGAGATTG+AGG 0.489163 8.2:+77924038 None:intergenic
TGAAGTAGTGAACGAGCTAA+AGG 0.502913 8.2:-77924140 MS.gene36689:CDS
GCAGATGCATGATATCAAAC+AGG 0.503492 8.2:-77924417 None:intergenic
AAATGAAGGTCCAATTGAGT+TGG 0.508594 8.2:-77924065 MS.gene36689:CDS
GAATTAGCCCGAAAAGGGGC+TGG 0.532477 8.2:-77924103 MS.gene36689:CDS
GAGTTCAGTTGTGAGATTGA+GGG 0.550230 8.2:+77924039 None:intergenic
TAGATCAATGCTTTATAATG+GGG 0.578982 8.2:-77924284 MS.gene36689:CDS
TCAATCTCACAACTGAACTC+GGG 0.605365 8.2:-77924036 MS.gene36689:CDS
TGTCCACAAATTCTTCCGAT+AGG 0.607903 8.2:-77924174 MS.gene36689:CDS
CAAGACTCACATAAGTCAGT+GGG 0.631950 8.2:-77924308 MS.gene36689:CDS
TGCAGAATTAGCCCGAAAAG+GGG 0.645278 8.2:-77924107 MS.gene36689:CDS
GAAAGCTGTTGAGATAGGAA+TGG 0.646564 8.2:-77924203 MS.gene36689:CDS
ACAAGACTCACATAAGTCAG+TGG 0.649111 8.2:-77924309 MS.gene36689:CDS
TTAGCCCGAAAAGGGGCTGG+TGG 0.663374 8.2:-77924100 MS.gene36689:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTTTTACTCACTTT+TGG - chr8.2:77924336-77924355 MS.gene36689:CDS 15.0%
!! TTAGATCAATGCTTTATAAT+GGG - chr8.2:77923974-77923993 MS.gene36689:intron 20.0%
!! TTATGAAACAGAAAATGAAA+TGG + chr8.2:77924309-77924328 None:intergenic 20.0%
!!! AAAAGTTATGTTTACAGTTT+TGG - chr8.2:77923874-77923893 MS.gene36689:CDS 20.0%
!!! ATTTTTAGCATCATTCATAA+AGG + chr8.2:77924265-77924284 None:intergenic 20.0%
! GTTAGATCAATGCTTTATAA+TGG - chr8.2:77923973-77923992 MS.gene36689:intron 25.0%
! TAGATCAATGCTTTATAATG+GGG - chr8.2:77923975-77923994 MS.gene36689:intron 25.0%
!! ACTGTAAACATAACTTTTCT+CGG + chr8.2:77923872-77923891 None:intergenic 25.0%
!!! ATTTTGATACTAACTATGCA+TGG - chr8.2:77924034-77924053 MS.gene36689:CDS 25.0%
ATAAAGGTTCTACCTAATTC+GGG + chr8.2:77924249-77924268 None:intergenic 30.0%
CATAAAGGTTCTACCTAATT+CGG + chr8.2:77924250-77924269 None:intergenic 30.0%
GGTGGTAAAACTAAAAATGA+AGG - chr8.2:77924180-77924199 MS.gene36689:CDS 30.0%
AAATGAAGGTCCAATTGAGT+TGG - chr8.2:77924194-77924213 MS.gene36689:CDS 35.0%
TTCACTACTTCACTCATGAT+TGG + chr8.2:77924111-77924130 None:intergenic 35.0%
!! CTTGTCAGGTGTTTTTGTTA+TGG + chr8.2:77923935-77923954 None:intergenic 35.0%
!!! CAGTTTTGGAGTTGTTCTTT+TGG - chr8.2:77923888-77923907 MS.gene36689:CDS 35.0%
ACAAGACTCACATAAGTCAG+TGG - chr8.2:77923950-77923969 MS.gene36689:intron 40.0%
CAAGACTCACATAAGTCAGT+GGG - chr8.2:77923951-77923970 MS.gene36689:intron 40.0%
CTCAATCTCACAACTGAACT+CGG - chr8.2:77924222-77924241 MS.gene36689:CDS 40.0%
GAAAGCTGTTGAGATAGGAA+TGG - chr8.2:77924056-77924075 MS.gene36689:CDS 40.0%
GAGTTCAGTTGTGAGATTGA+GGG + chr8.2:77924223-77924242 None:intergenic 40.0%
TCAATCTCACAACTGAACTC+GGG - chr8.2:77924223-77924242 MS.gene36689:CDS 40.0%
TGAAGTAGTGAACGAGCTAA+AGG - chr8.2:77924119-77924138 MS.gene36689:CDS 40.0%
TGTCCACAAATTCTTCCGAT+AGG - chr8.2:77924085-77924104 MS.gene36689:CDS 40.0%
TTGAGGGAAACCAACTCAAT+TGG + chr8.2:77924207-77924226 None:intergenic 40.0%
! TGACTTATGTGAGTCTTGTC+AGG + chr8.2:77923949-77923968 None:intergenic 40.0%
!! TCAGGTGTTTTTGTTATGGC+AGG + chr8.2:77923931-77923950 None:intergenic 40.0%
!! TTCACTCATGATTGGCCTAT+CGG + chr8.2:77924103-77924122 None:intergenic 40.0%
CGAGTTCAGTTGTGAGATTG+AGG + chr8.2:77924224-77924243 None:intergenic 45.0%
CTGCAGAATTAGCCCGAAAA+GGG - chr8.2:77924151-77924170 MS.gene36689:CDS 45.0%
GCATGGAAAGCTGTTGAGAT+AGG - chr8.2:77924051-77924070 MS.gene36689:CDS 45.0%
TGCAGAATTAGCCCGAAAAG+GGG - chr8.2:77924152-77924171 MS.gene36689:CDS 45.0%
TGGCCTATCGGAAGAATTTG+TGG + chr8.2:77924091-77924110 None:intergenic 45.0%
!! GAACGAGCTAAAGGAGTGTT+TGG - chr8.2:77924128-77924147 MS.gene36689:CDS 45.0%
GCTGCAGAATTAGCCCGAAA+AGG - chr8.2:77924150-77924169 MS.gene36689:CDS 50.0%
ACTGAACTCGGGCCCGAATT+AGG - chr8.2:77924234-77924253 MS.gene36689:CDS 55.0%
GAATTAGCCCGAAAAGGGGC+TGG - chr8.2:77924156-77924175 MS.gene36689:CDS 55.0%
! TACCACCACCAGCCCCTTTT+CGG + chr8.2:77924167-77924186 None:intergenic 55.0%
TTAGCCCGAAAAGGGGCTGG+TGG - chr8.2:77924159-77924178 MS.gene36689:CDS 60.0%
! ACCACCACCAGCCCCTTTTC+GGG + chr8.2:77924166-77924185 None:intergenic 60.0%
! GCCCGAAAAGGGGCTGGTGG+TGG - chr8.2:77924162-77924181 MS.gene36689:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 77923850 77924431 77923850 ID=MS.gene36689
chr8.2 mRNA 77923850 77924431 77923850 ID=MS.gene36689.t1;Parent=MS.gene36689
chr8.2 exon 77924026 77924431 77924026 ID=MS.gene36689.t1.exon1;Parent=MS.gene36689.t1
chr8.2 CDS 77924026 77924431 77924026 ID=cds.MS.gene36689.t1;Parent=MS.gene36689.t1
chr8.2 exon 77923850 77923914 77923850 ID=MS.gene36689.t1.exon2;Parent=MS.gene36689.t1
chr8.2 CDS 77923850 77923914 77923850 ID=cds.MS.gene36689.t1;Parent=MS.gene36689.t1
Gene Sequence

>MS.gene36689

ATGATATCAAACAGGTTAACCGAGAAAAGTTATGTTTACAGTTTTGGAGTTGTTCTTTTGGAGATAATCACTAGTCAACCTGCCATAACAAAAACACCTGACAAGACTCACATAAGTCAGTGGGTTAGATCAATGCTTTATAATGGGGATATAAAGAATATTGTTGACTCAAGATTACTACAAGATTTTGATACTAACTATGCATGGAAAGCTGTTGAGATAGGAATGGCTTGCGTGTCCACAAATTCTTCCGATAGGCCAATCATGAGTGAAGTAGTGAACGAGCTAAAGGAGTGTTTGGCTGCAGAATTAGCCCGAAAAGGGGCTGGTGGTGGTAAAACTAAAAATGAAGGTCCAATTGAGTTGGTTTCCCTCAATCTCACAACTGAACTCGGGCCCGAATTAGGTAGAACCTTTATGAATGATGCTAAAAATAATACTTTTGTTTGTTGTTGTCCATTTCATTTTCTGTTTCATAAGTATCTTATAATATTTTTACTCACTTTTGGTGTAACAGAGTGTGAAGTATTTTGTTGCAGTTCAATATCTATGTTTCTTTTAGTGAGAACTACTGAAGACTAA
Protein sequence

>MS.gene36689.t1

MISNRLTEKSYVYSFGVVLLEIITSQPAITKTPDKTHISQWVRSMLYNGDIKNIVDSRLLQDFDTNYAWKAVEIGMACVSTNSSDRPIMSEVVNELKECLAAELARKGAGGGKTKNEGPIELVSLNLTTELGPELECEVFCCSSISMFLLVRTTED