Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36985.t1 | KEH17998.1 | 81.7 | 180 | 33 | 0 | 1 | 180 | 17 | 196 | 1.00E-76 | 296.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36985.t1 | A0A072TMD2 | 81.7 | 180 | 33 | 0 | 1 | 180 | 17 | 196 | 7.5e-77 | 296.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36985.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36985.t1 | MTR_8g009730 | 81.667 | 180 | 33 | 0 | 1 | 180 | 17 | 196 | 6.32e-107 | 304 |
| MS.gene36985.t1 | MTR_8g445680 | 31.694 | 183 | 88 | 4 | 3 | 183 | 19 | 166 | 1.51e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCCAAGGATGTGCTTATT+TGG | 0.063042 | 8.3:-75718119 | None:intergenic |
| TCAGTGTGTCATTCAAATTT+TGG | 0.153883 | 8.3:+75717954 | MS.gene36985:CDS |
| CTGACTTAAACTTGAGTATA+TGG | 0.197296 | 8.3:+75718235 | MS.gene36985:CDS |
| ATCCAAGGATGTGCTTATTT+GGG | 0.326625 | 8.3:-75718118 | None:intergenic |
| CATGACACTGACTGTGAATT+TGG | 0.372038 | 8.3:+75717898 | MS.gene36985:CDS |
| AAAGTCTTCATCCTCATTAC+TGG | 0.397078 | 8.3:-75718730 | None:intergenic |
| TCTTGAACACCAATTGGTGC+TGG | 0.399679 | 8.3:+75718092 | MS.gene36985:CDS |
| GGGAATGGTGGCTGATGAAA+AGG | 0.430935 | 8.3:+75718677 | MS.gene36985:CDS |
| TGACTTAAACTTGAGTATAT+GGG | 0.443514 | 8.3:+75718236 | MS.gene36985:CDS |
| TTCATCAGCCACCATTCCCT+TGG | 0.455807 | 8.3:-75718673 | None:intergenic |
| TTTGGGCACCCAGCACCAAT+TGG | 0.466511 | 8.3:-75718101 | None:intergenic |
| TTGGCGAAGTAACTCAGATC+GGG | 0.471484 | 8.3:+75718065 | MS.gene36985:CDS |
| TGCCCAAATAAGCACATCCT+TGG | 0.475102 | 8.3:+75718116 | MS.gene36985:CDS |
| AGCAATTTCAATTTCATCCA+AGG | 0.478006 | 8.3:-75718133 | None:intergenic |
| CATATACTCAAGTTTAAGTC+AGG | 0.479889 | 8.3:-75718234 | None:intergenic |
| CTTGAACACCAATTGGTGCT+GGG | 0.482127 | 8.3:+75718093 | MS.gene36985:CDS |
| CTGAAAATCAGCATGCCTAC+TGG | 0.485199 | 8.3:-75717935 | None:intergenic |
| TGTGCCAACATATGAGAAGA+AGG | 0.488999 | 8.3:+75717875 | None:intergenic |
| TTAATCGCATAATTCTCATC+AGG | 0.493767 | 8.3:-75717987 | None:intergenic |
| TCTCCTCTTTCACAAGTACA+TGG | 0.497829 | 8.3:-75718485 | None:intergenic |
| TCAGGAATTTGAGTGCTAGA+AGG | 0.498016 | 8.3:-75718216 | None:intergenic |
| TCCTTCTGTGAGAAGCGAAT+CGG | 0.511515 | 8.3:-75718037 | None:intergenic |
| TACTGAACACATGGGTGACA+TGG | 0.514848 | 8.3:+75718433 | MS.gene36985:CDS |
| GCCGATTCGCTTCTCACAGA+AGG | 0.517177 | 8.3:+75718036 | MS.gene36985:CDS |
| ATTCCATGTACTTGTGAAAG+AGG | 0.523001 | 8.3:+75718482 | MS.gene36985:CDS |
| GTGGCTGATGAAAAGGGAAA+AGG | 0.537842 | 8.3:+75718684 | MS.gene36985:CDS |
| CTTGGCGAAGTAACTCAGAT+CGG | 0.541406 | 8.3:+75718064 | MS.gene36985:CDS |
| GTCTTCATCCTCATTACTGG+TGG | 0.547436 | 8.3:-75718727 | None:intergenic |
| TGTACACAGTAATCTATCCA+AGG | 0.549486 | 8.3:+75718656 | MS.gene36985:intron |
| TTCTCACAGAAGGAGAAGCT+TGG | 0.549947 | 8.3:+75718046 | MS.gene36985:CDS |
| GTGCTAGAAGGCATGGAAGA+AGG | 0.561567 | 8.3:-75718204 | None:intergenic |
| CAGTAATCTATCCAAGGGAA+TGG | 0.569736 | 8.3:+75718662 | MS.gene36985:intron |
| AATTTGAGTGCTAGAAGGCA+TGG | 0.572126 | 8.3:-75718211 | None:intergenic |
| GCAACAACTTGTCTTTGATG+TGG | 0.579471 | 8.3:-75718015 | None:intergenic |
| TAATCTATCCAAGGGAATGG+TGG | 0.591476 | 8.3:+75718665 | MS.gene36985:CDS |
| ACAAGGACCCACCAGTAATG+AGG | 0.613595 | 8.3:+75718719 | MS.gene36985:CDS |
| CTTGTGAAAGAGGAGAATCA+AGG | 0.617523 | 8.3:+75718492 | MS.gene36985:CDS |
| GGAATGGTGGCTGATGAAAA+GGG | 0.618900 | 8.3:+75718678 | MS.gene36985:CDS |
| CATGGGTGACATGGACATCA+CGG | 0.621521 | 8.3:+75718442 | MS.gene36985:CDS |
| TTAATAGAGTACTGAACACA+TGG | 0.626411 | 8.3:+75718424 | MS.gene36985:intron |
| GTACACAGTAATCTATCCAA+GGG | 0.631300 | 8.3:+75718657 | MS.gene36985:intron |
| TCTTCATCCTCATTACTGGT+GGG | 0.637097 | 8.3:-75718726 | None:intergenic |
| AAAGGAATTCTTAATGAACA+AGG | 0.657272 | 8.3:+75718702 | MS.gene36985:CDS |
| TGGCGAAGTAACTCAGATCG+GGG | 0.662365 | 8.3:+75718066 | MS.gene36985:CDS |
| ATCACGGAACATGACATGCA+AGG | 0.668838 | 8.3:+75718458 | MS.gene36985:CDS |
| TAATAGAGTACTGAACACAT+GGG | 0.740993 | 8.3:+75718425 | MS.gene36985:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATATAAAATGTGTATT+AGG | - | chr8.3:75718632-75718651 | None:intergenic | 10.0% |
| ! | AAAGGAATTCTTAATGAACA+AGG | + | chr8.3:75718702-75718721 | MS.gene36985:CDS | 25.0% |
| ! | TGACTTAAACTTGAGTATAT+GGG | + | chr8.3:75718236-75718255 | MS.gene36985:CDS | 25.0% |
| ! | TTGAAAAGAATGATAGATCT+TGG | - | chr8.3:75718380-75718399 | None:intergenic | 25.0% |
| AGCAATTTCAATTTCATCCA+AGG | - | chr8.3:75718136-75718155 | None:intergenic | 30.0% | |
| ATGTGTTCAGTACTCTATTA+AGG | - | chr8.3:75718425-75718444 | None:intergenic | 30.0% | |
| CATATACTCAAGTTTAAGTC+AGG | - | chr8.3:75718237-75718256 | None:intergenic | 30.0% | |
| CTGACTTAAACTTGAGTATA+TGG | + | chr8.3:75718235-75718254 | MS.gene36985:CDS | 30.0% | |
| TTAATCGCATAATTCTCATC+AGG | - | chr8.3:75717990-75718009 | None:intergenic | 30.0% | |
| !! | AGTGTGTCATTCAAATTTTG+GGG | + | chr8.3:75717956-75717975 | MS.gene36985:CDS | 30.0% |
| !! | CAGTGTGTCATTCAAATTTT+GGG | + | chr8.3:75717955-75717974 | MS.gene36985:CDS | 30.0% |
| !! | TAATAGAGTACTGAACACAT+GGG | + | chr8.3:75718425-75718444 | MS.gene36985:intron | 30.0% |
| !! | TCAGTGTGTCATTCAAATTT+TGG | + | chr8.3:75717954-75717973 | MS.gene36985:CDS | 30.0% |
| !! | TTAATAGAGTACTGAACACA+TGG | + | chr8.3:75718424-75718443 | MS.gene36985:intron | 30.0% |
| AAAGTCTTCATCCTCATTAC+TGG | - | chr8.3:75718733-75718752 | None:intergenic | 35.0% | |
| ATCCAAGGATGTGCTTATTT+GGG | - | chr8.3:75718121-75718140 | None:intergenic | 35.0% | |
| ATTCCATGTACTTGTGAAAG+AGG | + | chr8.3:75718482-75718501 | MS.gene36985:CDS | 35.0% | |
| GTACACAGTAATCTATCCAA+GGG | + | chr8.3:75718657-75718676 | MS.gene36985:intron | 35.0% | |
| TGTACACAGTAATCTATCCA+AGG | + | chr8.3:75718656-75718675 | MS.gene36985:intron | 35.0% | |
| AATTTGAGTGCTAGAAGGCA+TGG | - | chr8.3:75718214-75718233 | None:intergenic | 40.0% | |
| CAGTAATCTATCCAAGGGAA+TGG | + | chr8.3:75718662-75718681 | MS.gene36985:intron | 40.0% | |
| CATCCAAGGATGTGCTTATT+TGG | - | chr8.3:75718122-75718141 | None:intergenic | 40.0% | |
| CTTGTGAAAGAGGAGAATCA+AGG | + | chr8.3:75718492-75718511 | MS.gene36985:CDS | 40.0% | |
| GCAACAACTTGTCTTTGATG+TGG | - | chr8.3:75718018-75718037 | None:intergenic | 40.0% | |
| TAATCTATCCAAGGGAATGG+TGG | + | chr8.3:75718665-75718684 | MS.gene36985:CDS | 40.0% | |
| TCAGGAATTTGAGTGCTAGA+AGG | - | chr8.3:75718219-75718238 | None:intergenic | 40.0% | |
| TCTCCTCTTTCACAAGTACA+TGG | - | chr8.3:75718488-75718507 | None:intergenic | 40.0% | |
| TCTTCATCCTCATTACTGGT+GGG | - | chr8.3:75718729-75718748 | None:intergenic | 40.0% | |
| ! | CATGACACTGACTGTGAATT+TGG | + | chr8.3:75717898-75717917 | MS.gene36985:CDS | 40.0% |
| ! | GGGTTTTCTTGAACACCAAT+TGG | + | chr8.3:75718086-75718105 | MS.gene36985:CDS | 40.0% |
| ! | GTCAATACTTTTCGACCAGT+AGG | + | chr8.3:75717920-75717939 | MS.gene36985:CDS | 40.0% |
| ATCACGGAACATGACATGCA+AGG | + | chr8.3:75718458-75718477 | MS.gene36985:CDS | 45.0% | |
| CTGAAAATCAGCATGCCTAC+TGG | - | chr8.3:75717938-75717957 | None:intergenic | 45.0% | |
| CTTGGCGAAGTAACTCAGAT+CGG | + | chr8.3:75718064-75718083 | MS.gene36985:CDS | 45.0% | |
| GGAATGGTGGCTGATGAAAA+GGG | + | chr8.3:75718678-75718697 | MS.gene36985:CDS | 45.0% | |
| GTCTTCATCCTCATTACTGG+TGG | - | chr8.3:75718730-75718749 | None:intergenic | 45.0% | |
| GTGGCTGATGAAAAGGGAAA+AGG | + | chr8.3:75718684-75718703 | MS.gene36985:CDS | 45.0% | |
| TACTGAACACATGGGTGACA+TGG | + | chr8.3:75718433-75718452 | MS.gene36985:CDS | 45.0% | |
| TCCTTCTGTGAGAAGCGAAT+CGG | - | chr8.3:75718040-75718059 | None:intergenic | 45.0% | |
| TCTTGAACACCAATTGGTGC+TGG | + | chr8.3:75718092-75718111 | MS.gene36985:CDS | 45.0% | |
| TGCCCAAATAAGCACATCCT+TGG | + | chr8.3:75718116-75718135 | MS.gene36985:CDS | 45.0% | |
| TTCTCACAGAAGGAGAAGCT+TGG | + | chr8.3:75718046-75718065 | MS.gene36985:CDS | 45.0% | |
| TTGGCGAAGTAACTCAGATC+GGG | + | chr8.3:75718065-75718084 | MS.gene36985:CDS | 45.0% | |
| ! | CTTGAACACCAATTGGTGCT+GGG | + | chr8.3:75718093-75718112 | MS.gene36985:CDS | 45.0% |
| ACAAGGACCCACCAGTAATG+AGG | + | chr8.3:75718719-75718738 | MS.gene36985:CDS | 50.0% | |
| CATGGGTGACATGGACATCA+CGG | + | chr8.3:75718442-75718461 | MS.gene36985:CDS | 50.0% | |
| GGGAATGGTGGCTGATGAAA+AGG | + | chr8.3:75718677-75718696 | MS.gene36985:CDS | 50.0% | |
| TGGCGAAGTAACTCAGATCG+GGG | + | chr8.3:75718066-75718085 | MS.gene36985:CDS | 50.0% | |
| TTCATCAGCCACCATTCCCT+TGG | - | chr8.3:75718676-75718695 | None:intergenic | 50.0% | |
| ! | GTGCTAGAAGGCATGGAAGA+AGG | - | chr8.3:75718207-75718226 | None:intergenic | 50.0% |
| TTTGGGCACCCAGCACCAAT+TGG | - | chr8.3:75718104-75718123 | None:intergenic | 55.0% | |
| !! | GCCGATTCGCTTCTCACAGA+AGG | + | chr8.3:75718036-75718055 | MS.gene36985:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 75717886 | 75718760 | 75717886 | ID=MS.gene36985 |
| chr8.3 | mRNA | 75717886 | 75718760 | 75717886 | ID=MS.gene36985.t1;Parent=MS.gene36985 |
| chr8.3 | exon | 75717886 | 75718257 | 75717886 | ID=MS.gene36985.t1.exon1;Parent=MS.gene36985.t1 |
| chr8.3 | CDS | 75717886 | 75718257 | 75717886 | ID=cds.MS.gene36985.t1;Parent=MS.gene36985.t1 |
| chr8.3 | exon | 75718431 | 75718513 | 75718431 | ID=MS.gene36985.t1.exon2;Parent=MS.gene36985.t1 |
| chr8.3 | CDS | 75718431 | 75718513 | 75718431 | ID=cds.MS.gene36985.t1;Parent=MS.gene36985.t1 |
| chr8.3 | exon | 75718665 | 75718760 | 75718665 | ID=MS.gene36985.t1.exon3;Parent=MS.gene36985.t1 |
| chr8.3 | CDS | 75718665 | 75718760 | 75718665 | ID=cds.MS.gene36985.t1;Parent=MS.gene36985.t1 |
| Gene Sequence |
| Protein sequence |
