AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37066

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37066.t1 MTR_1g053800 90.994 322 26 2 1 321 1 320 0.0 585
MS.gene37066.t1 MTR_7g093010 44.964 278 119 9 52 314 90 348 2.10e-55 184
MS.gene37066.t1 MTR_1g053835 51.852 135 64 1 174 308 254 387 1.05e-34 131
MS.gene37066.t1 MTR_7g093030 51.145 131 64 0 178 308 212 342 1.67e-33 127
MS.gene37066.t1 MTR_1g080330 45.775 142 76 1 168 308 165 306 6.73e-33 125
MS.gene37066.t1 MTR_7g098250 44.667 150 75 3 159 306 248 391 3.34e-32 125
MS.gene37066.t1 MTR_2g027860 47.445 137 63 2 178 308 49 182 1.06e-30 118
MS.gene37066.t1 MTR_7g115530 43.939 132 73 1 178 308 218 349 8.10e-28 112
MS.gene37066.t1 MTR_7g088070 40.299 134 80 0 175 308 47 180 9.78e-28 109
MS.gene37066.t1 MTR_7g088070 39.437 142 74 2 175 308 47 184 8.27e-26 104
MS.gene37066.t1 MTR_6g444980 38.608 158 85 2 167 316 24 177 8.68e-25 103
MS.gene37066.t1 MTR_6g444980 40.845 142 72 2 167 300 24 161 6.62e-24 100
MS.gene37066.t1 MTR_2g086450 38.596 171 84 6 166 315 33 203 6.72e-24 100
MS.gene37066.t1 MTR_1g080330 43.220 118 66 1 168 284 165 282 7.86e-24 99.0
MS.gene37066.t1 MTR_6g032990 37.580 157 73 3 173 308 37 189 6.61e-23 98.2
MS.gene37066.t1 MTR_5g027440 36.306 157 81 3 178 318 25 178 1.34e-20 90.1
MS.gene37066.t1 MTR_4g081710 37.931 145 74 3 173 303 12 154 2.55e-20 90.1
MS.gene37066.t1 MTR_5g041350 37.584 149 74 3 171 303 8 153 2.94e-20 90.1
MS.gene37066.t1 MTR_7g115530 42.202 109 62 1 178 285 218 326 5.83e-20 89.0
MS.gene37066.t1 MTR_7g068600 39.716 141 81 2 174 310 9 149 9.99e-20 86.7
MS.gene37066.t1 MTR_2g027800 37.313 134 75 2 173 302 34 162 1.20e-18 85.1
MS.gene37066.t1 MTR_7g093030 51.220 82 40 0 178 259 212 293 3.42e-18 83.6
MS.gene37066.t1 MTR_7g115530 55.882 68 30 0 178 245 218 285 4.03e-18 84.3
MS.gene37066.t1 MTR_2g027800 34.965 143 80 3 173 302 34 176 1.74e-17 82.0
MS.gene37066.t1 MTR_7g115530 62.500 56 21 0 178 233 218 273 1.99e-17 81.6
MS.gene37066.t1 MTR_1g090670 33.103 145 94 3 165 308 31 173 3.87e-16 77.8
MS.gene37066.t1 MTR_7g115530 63.462 52 19 0 178 229 218 269 2.36e-15 75.1
MS.gene37066.t1 MTR_1g053830 50.746 67 33 0 180 246 20 86 1.38e-14 68.9
MS.gene37066.t1 MTR_8g086410 51.429 70 26 1 180 249 111 172 1.55e-14 74.3
MS.gene37066.t1 MTR_8g086410 51.429 70 26 1 180 249 113 174 1.57e-14 74.3
MS.gene37066.t1 MTR_7g115530 60.377 53 21 0 178 230 218 270 1.61e-14 73.2
MS.gene37066.t1 MTR_0223s0040 62.264 53 20 0 180 232 20 72 2.30e-14 72.4
MS.gene37066.t1 MTR_2g016220 52.857 70 25 1 180 249 91 152 3.03e-14 73.2
MS.gene37066.t1 MTR_2g016220 52.857 70 25 1 180 249 91 152 3.05e-14 73.2
MS.gene37066.t1 MTR_2g084230 51.429 70 26 1 180 249 99 160 5.85e-14 72.4
MS.gene37066.t1 MTR_2g084230 51.429 70 26 1 180 249 99 160 6.10e-14 71.6
MS.gene37066.t1 MTR_8g086410 47.143 70 36 1 180 249 113 181 8.53e-14 72.0
MS.gene37066.t1 MTR_1g112370 60.377 53 21 0 180 232 24 76 1.31e-13 70.9
MS.gene37066.t1 MTR_3g450310 59.259 54 22 0 180 233 21 74 1.39e-13 68.9
MS.gene37066.t1 MTR_8g066310 45.783 83 40 1 156 233 146 228 1.68e-13 70.9
MS.gene37066.t1 MTR_2g023580 58.491 53 22 0 180 232 57 109 3.30e-13 68.6
MS.gene37066.t1 MTR_7g069660 56.604 53 23 0 180 232 73 125 7.22e-13 68.9
MS.gene37066.t1 MTR_6g071625 50.847 59 29 0 180 238 204 262 1.07e-12 68.6
MS.gene37066.t1 MTR_1g021520 47.368 76 36 2 180 254 128 200 1.34e-12 67.4
MS.gene37066.t1 MTR_3g064840 38.532 109 51 3 180 288 276 368 1.97e-12 68.2
MS.gene37066.t1 MTR_2g016220 50.000 74 25 2 180 249 91 156 2.58e-12 67.4
MS.gene37066.t1 MTR_5g029470 48.333 60 31 0 180 239 221 280 7.65e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37066.t1 AT5G06800 42.941 170 94 1 139 308 156 322 8.40e-35 130
MS.gene37066.t1 AT5G06800 42.286 175 93 2 139 308 156 327 1.99e-34 129
MS.gene37066.t1 AT4G28610 46.528 144 72 2 165 306 215 355 1.15e-31 122
MS.gene37066.t1 AT2G01060 48.175 137 61 2 178 308 15 147 1.29e-30 117
MS.gene37066.t1 AT5G29000 43.165 139 75 2 174 308 227 365 5.95e-29 115
MS.gene37066.t1 AT5G29000 43.165 139 75 2 174 308 184 322 6.54e-29 114
MS.gene37066.t1 AT5G29000 43.165 139 75 2 174 308 184 322 6.54e-29 114
MS.gene37066.t1 AT3G04450 40.426 141 80 1 178 314 238 378 7.31e-28 112
MS.gene37066.t1 AT3G04450 40.426 141 80 1 178 314 198 338 7.36e-28 112
MS.gene37066.t1 AT3G04450 40.426 141 80 1 178 314 198 338 7.36e-28 112
MS.gene37066.t1 AT3G13040 39.744 156 78 2 164 308 227 377 3.79e-24 102
MS.gene37066.t1 AT3G13040 39.744 156 78 2 164 308 227 377 3.79e-24 102
MS.gene37066.t1 AT3G13040 39.744 156 78 2 164 308 227 377 3.79e-24 102
MS.gene37066.t1 AT4G13640 37.736 159 82 3 173 316 32 188 6.67e-24 99.4
MS.gene37066.t1 AT1G79430 38.854 157 84 2 167 315 23 175 1.80e-23 99.4
MS.gene37066.t1 AT2G20400 41.985 131 74 1 178 306 231 361 5.80e-23 98.6
MS.gene37066.t1 AT5G18240 37.179 156 82 2 166 305 33 188 7.55e-23 98.2
MS.gene37066.t1 AT5G18240 37.179 156 82 2 166 305 33 188 7.55e-23 98.2
MS.gene37066.t1 AT3G04030 34.940 166 74 2 165 305 32 188 1.29e-22 97.4
MS.gene37066.t1 AT2G20400 42.748 131 72 2 178 306 231 360 1.34e-22 97.4
MS.gene37066.t1 AT3G24120 39.490 157 82 2 173 316 36 192 2.13e-22 95.5
MS.gene37066.t1 AT4G13640 37.037 162 82 4 173 316 32 191 6.44e-22 94.0
MS.gene37066.t1 AT3G24120 38.750 160 82 3 173 316 36 195 2.16e-20 89.7
MS.gene37066.t1 AT5G18240 36.538 156 81 3 166 305 33 186 2.52e-20 90.9
MS.gene37066.t1 AT3G12730 35.135 148 87 2 167 305 12 159 3.13e-20 88.2
MS.gene37066.t1 AT3G04030 33.735 166 75 3 165 305 32 187 1.16e-19 89.0
MS.gene37066.t1 AT1G69580 36.913 149 76 2 173 303 29 177 7.22e-19 86.3
MS.gene37066.t1 AT5G18240 33.974 156 81 3 166 305 33 182 3.82e-18 84.7
MS.gene37066.t1 AT5G18240 33.974 156 81 3 166 305 33 182 3.82e-18 84.7
MS.gene37066.t1 AT1G69580 35.065 154 73 2 173 303 29 178 6.44e-18 83.6
MS.gene37066.t1 AT3G04030 31.928 166 73 3 165 305 32 182 6.81e-18 84.0
MS.gene37066.t1 AT5G45580 31.902 163 86 3 168 308 13 172 1.56e-17 81.6
MS.gene37066.t1 AT5G45580 32.680 153 78 3 178 308 7 156 3.83e-17 80.1
MS.gene37066.t1 AT5G29000 58.333 60 25 0 174 233 227 286 7.09e-17 80.1
MS.gene37066.t1 AT5G06800 44.681 94 49 1 138 231 155 245 2.93e-16 78.2
MS.gene37066.t1 AT3G04450 55.172 58 26 0 178 235 238 295 5.40e-16 77.8
MS.gene37066.t1 AT2G42660 58.491 53 22 0 180 232 52 104 1.47e-14 72.8
MS.gene37066.t1 AT4G04605 60.377 53 21 0 180 232 16 68 2.93e-14 71.2
MS.gene37066.t1 AT2G40260 60.377 53 21 0 180 232 84 136 4.00e-14 72.8
MS.gene37066.t1 AT5G42630 43.478 92 46 3 160 249 86 173 4.01e-14 71.6
MS.gene37066.t1 AT5G42630 43.478 92 46 3 160 249 86 173 4.24e-14 70.9
MS.gene37066.t1 AT1G14600 61.111 54 21 0 180 233 25 78 6.75e-14 70.9
MS.gene37066.t1 AT2G01060 45.098 102 46 2 213 308 1 98 8.27e-14 70.5
MS.gene37066.t1 AT4G04580 58.491 53 22 0 180 232 16 68 8.79e-14 68.6
MS.gene37066.t1 AT5G16560 41.111 90 53 0 180 269 221 310 9.77e-14 71.6
MS.gene37066.t1 AT2G38300 46.753 77 33 1 180 256 56 124 2.37e-13 70.1
MS.gene37066.t1 AT2G02060 58.491 53 22 0 180 232 31 83 1.04e-12 67.4
MS.gene37066.t1 AT2G02060 58.491 53 22 0 180 232 31 83 1.04e-12 67.4
MS.gene37066.t1 AT4G17695 50.000 70 32 2 172 238 155 224 1.38e-12 67.8
MS.gene37066.t1 AT1G32240 36.190 105 61 2 180 278 215 319 1.99e-12 67.8
MS.gene37066.t1 AT1G32240 36.190 105 61 2 180 278 215 319 2.01e-12 67.8
MS.gene37066.t1 AT2G06020 48.276 58 30 0 174 231 82 139 2.20e-12 67.0
MS.gene37066.t1 AT4G04555 58.824 51 21 0 182 232 18 68 8.47e-12 61.6
MS.gene37066.t1 AT4G37180 45.333 75 40 1 163 237 196 269 1.40e-11 65.1
MS.gene37066.t1 AT4G37180 45.333 75 40 1 163 237 203 276 1.76e-11 64.7

Find 55 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAACAACCTGCTTCAAAT+TGG 0.204478 1.4:+39854389 MS.gene37066:CDS
CAGCCTTGACAATACATAAA+AGG 0.269493 1.4:-39854327 None:intergenic
ATGAGTTTGACATACCTATT+TGG 0.292306 1.4:+39854268 MS.gene37066:CDS
GTGATTCTTCTATTTGCATT+TGG 0.336254 1.4:-39856827 None:intergenic
ATTTCATGGGACCTCTGATC+TGG 0.337576 1.4:-39854519 None:intergenic
TATCTTAAAATCAGAGTTGC+TGG 0.350060 1.4:-39855510 None:intergenic
TCATGTCAAAAGTCATTTGC+AGG 0.353917 1.4:+39855767 MS.gene37066:CDS
TTCCCTCTTGTTCTAATAAG+AGG 0.359635 1.4:+39854475 MS.gene37066:CDS
CTAAAATGTACATAAATCAT+TGG 0.360570 1.4:+39854121 None:intergenic
ATTATATAAACTTACTTTCT+GGG 0.366001 1.4:-39855613 None:intergenic
GAAAATTCATTGCTCCAAAT+AGG 0.375788 1.4:-39854282 None:intergenic
CCAACAACCTGCTTCAAATT+GGG 0.382315 1.4:+39854390 MS.gene37066:CDS
CCCAATTTGAAGCAGGTTGT+TGG 0.384228 1.4:-39854390 None:intergenic
GGTTGTTGGTTGATCTTGTT+TGG 0.395689 1.4:-39854376 None:intergenic
TAAAATGTACATAAATCATT+GGG 0.405097 1.4:+39854122 None:intergenic
TTGATCTTGAAAATTCATAA+AGG 0.413952 1.4:-39854232 None:intergenic
ACAAAAGAAAAGAACCAAAC+AGG 0.442809 1.4:+39857096 MS.gene37066:CDS
ATCATTTGTAGTATAGCCTT+AGG 0.450838 1.4:-39855720 None:intergenic
TAAGGCTATACTACAAATGA+TGG 0.452684 1.4:+39855722 MS.gene37066:CDS
TTTCATGGGACCTCTGATCT+GGG 0.460927 1.4:-39854518 None:intergenic
TTCAGAACCCAATTTGAAGC+AGG 0.463730 1.4:-39854397 None:intergenic
TACTTTCTGGGCCACCAAGC+TGG 0.476548 1.4:-39855601 None:intergenic
ATTATTATTGTCAACCAGCT+TGG 0.483051 1.4:+39855587 MS.gene37066:CDS
TAAAGGTTTCATTTGCTACA+AGG 0.488944 1.4:-39854215 None:intergenic
TTGTTCTAATAAGAGGGTGC+TGG 0.513933 1.4:+39854482 MS.gene37066:CDS
TCCCTCTTGTTCTAATAAGA+GGG 0.514383 1.4:+39854476 MS.gene37066:CDS
GATGCAGCGAAATTTGCAAC+TGG 0.519384 1.4:+39857030 MS.gene37066:intron
ATTATTGTCAACCAGCTTGG+TGG 0.523219 1.4:+39855590 MS.gene37066:CDS
CACACGCACAAATGTTTGAA+AGG 0.526179 1.4:+39856058 MS.gene37066:CDS
AAAGGTTTCATTTGCTACAA+GGG 0.527456 1.4:-39854214 None:intergenic
TTTCTGAAAGGAAATTTCAT+GGG 0.532722 1.4:-39854532 None:intergenic
AGGGTAAAAGAAGATTAAGA+TGG 0.538823 1.4:+39855540 MS.gene37066:CDS
GAGGAAGGGCAGATAACAGA+TGG 0.546455 1.4:+39856672 MS.gene37066:CDS
GAGCAATAAACGACAATTCG+AGG 0.546674 1.4:+39856875 MS.gene37066:CDS
CACCCTCTTATTAGAACAAG+AGG 0.558672 1.4:-39854478 None:intergenic
AAAGGTGAATCTGTTGTTGT+TGG 0.559028 1.4:-39854309 None:intergenic
TTAATCAGAGGCAAAGCCTA+AGG 0.566249 1.4:+39855704 MS.gene37066:intron
AACTACAACTTGAGGTTGAG+AGG 0.574473 1.4:+39856853 MS.gene37066:CDS
AGAGGACTAACTATGCTACA+AGG 0.576073 1.4:-39854168 None:intergenic
GTTTCAGGAATATCTGAGGA+AGG 0.576924 1.4:+39856657 MS.gene37066:intron
AATTTCCTTTCAGAAAACTC+AGG 0.594791 1.4:+39854539 MS.gene37066:CDS
GACAAACAATCTTCACCTTG+TGG 0.605075 1.4:+39855470 MS.gene37066:CDS
AAGGCTATACTACAAATGAT+GGG 0.610409 1.4:+39855723 MS.gene37066:CDS
GCATCTGATATGTCCTCTAG+AGG 0.615226 1.4:-39854186 None:intergenic
TAGCATAGTTAGTCCTCTAG+AGG 0.629733 1.4:+39854173 MS.gene37066:CDS
TTTCAGGAATATCTGAGGAA+GGG 0.636689 1.4:+39856658 MS.gene37066:intron
TTGTGTTTCAGGAATATCTG+AGG 0.637900 1.4:+39856653 MS.gene37066:intron
ATCACGACAACTACAACTTG+AGG 0.638258 1.4:+39856845 MS.gene37066:CDS
TTCATGGGACCTCTGATCTG+GGG 0.643024 1.4:-39854517 None:intergenic
GAGGACTAACTATGCTACAA+GGG 0.646307 1.4:-39854167 None:intergenic
TATACTACAAATGATGGGTG+TGG 0.647886 1.4:+39855728 MS.gene37066:CDS
GTATGTCAAACTCATGCTGA+TGG 0.650055 1.4:-39854260 None:intergenic
AGGAAGGGCAGATAACAGAT+GGG 0.654049 1.4:+39856673 MS.gene37066:CDS
ACCCTCTTATTAGAACAAGA+GGG 0.671675 1.4:-39854477 None:intergenic
GTTGCAAAATCAAAGCCACA+AGG 0.709667 1.4:-39855485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAGATTCA+TGG - chr1.4:39856385-39856404 None:intergenic 10.0%
!! AAATTAATTAAAAATAGAGA+AGG + chr1.4:39856301-39856320 MS.gene37066:intron 10.0%
!! AATAAATTAATATATATTGG+AGG - chr1.4:39856635-39856654 None:intergenic 10.0%
!! AATTAATTAAAAATAGAGAA+GGG + chr1.4:39856302-39856321 MS.gene37066:intron 10.0%
!! CACAATAAATTAATATATAT+TGG - chr1.4:39856638-39856657 None:intergenic 10.0%
!!! ATATTTTATATCAAGAATTA+AGG + chr1.4:39856965-39856984 MS.gene37066:intron 10.0%
!!! GTTTATATAATTCATTTTAT+AGG + chr1.4:39855625-39855644 MS.gene37066:intron 10.0%
!!! TTTTTTTTTTCAAATATTAG+TGG + chr1.4:39856404-39856423 MS.gene37066:intron 10.0%
!! AATTATATAAACTTACTTTC+TGG - chr1.4:39855617-39855636 None:intergenic 15.0%
!! ATATTAATTTATTGTGTTTC+AGG + chr1.4:39856642-39856661 MS.gene37066:intron 15.0%
!! ATTATATAAACTTACTTTCT+GGG - chr1.4:39855616-39855635 None:intergenic 15.0%
!! TAATAATTTATTAGCGATAT+GGG + chr1.4:39855959-39855978 MS.gene37066:intron 15.0%
!! TTAAGTATAATTATATGTGT+CGG - chr1.4:39855928-39855947 None:intergenic 15.0%
!! TTAATAATTTATTAGCGATA+TGG + chr1.4:39855958-39855977 MS.gene37066:intron 15.0%
!! TTGAAATTGAAAACTAAATT+TGG - chr1.4:39856158-39856177 None:intergenic 15.0%
!!! ATTATTGATTACAATGAATT+TGG + chr1.4:39855007-39855026 MS.gene37066:intron 15.0%
!!! TTATTGATTACAATGAATTT+GGG + chr1.4:39855008-39855027 MS.gene37066:intron 15.0%
!! AATTTGAAGAGTAATAAGAA+AGG - chr1.4:39854588-39854607 None:intergenic 20.0%
!! ATCTTAAATATGTATGATGA+CGG + chr1.4:39855235-39855254 MS.gene37066:intron 20.0%
!! ATTATTTACATCAAGAATTC+AGG + chr1.4:39856911-39856930 MS.gene37066:intron 20.0%
!! TAGAAATTCATGAATAAGTA+GGG + chr1.4:39856428-39856447 MS.gene37066:intron 20.0%
!! TTAATATATATTGGAGGTTA+AGG - chr1.4:39856629-39856648 None:intergenic 20.0%
!! TTATAATTTACCCTCAAAAT+AGG - chr1.4:39855835-39855854 None:intergenic 20.0%
!! TTGATCTTGAAAATTCATAA+AGG - chr1.4:39854235-39854254 None:intergenic 20.0%
!!! ATTACTCTTCAAATTTTTGT+TGG + chr1.4:39854593-39854612 MS.gene37066:intron 20.0%
!!! GAAACAATTATTTGTTAAAG+AGG - chr1.4:39854842-39854861 None:intergenic 20.0%
!!! TAATAAAATGATACCCTTTT+AGG - chr1.4:39855062-39855081 None:intergenic 20.0%
!!! TCATCATACATATTTAAGAT+TGG - chr1.4:39855235-39855254 None:intergenic 20.0%
!!! TGATTATTTATTTTGTCTTG+TGG + chr1.4:39857004-39857023 MS.gene37066:intron 20.0%
!!! TTTAATCATTGATTAATCAG+AGG + chr1.4:39855692-39855711 MS.gene37066:intron 20.0%
! AAACTTCTAAATGGATCATT+GGG - chr1.4:39855369-39855388 None:intergenic 25.0%
! AACCATTGTTAATTAGTCAA+TGG + chr1.4:39854620-39854639 MS.gene37066:intron 25.0%
! AACCTTAAAATTATGTGCAT+TGG + chr1.4:39856740-39856759 MS.gene37066:intron 25.0%
! AAGCAATATGTATACCTAAA+AGG + chr1.4:39855045-39855064 MS.gene37066:intron 25.0%
! ACTATGATAAGACATTTATG+AGG - chr1.4:39855430-39855449 None:intergenic 25.0%
! AGCAATATGTATACCTAAAA+GGG + chr1.4:39855046-39855065 MS.gene37066:intron 25.0%
! ATTAGTCAATGGATGAATTT+AGG + chr1.4:39854631-39854650 MS.gene37066:intron 25.0%
! CAAGAGTCATATAGAAATTA+AGG - chr1.4:39854959-39854978 None:intergenic 25.0%
! CATAAATGTCTTATCATAGT+GGG + chr1.4:39855430-39855449 MS.gene37066:intron 25.0%
! CTAGAAATTCATGAATAAGT+AGG + chr1.4:39856427-39856446 MS.gene37066:intron 25.0%
! GGTTAAAATGCATTTCATAA+AGG - chr1.4:39856137-39856156 None:intergenic 25.0%
! GTTACATAGAAACTTCTAAA+TGG - chr1.4:39855378-39855397 None:intergenic 25.0%
! TAATTGACTACAGTTTCAAT+AGG + chr1.4:39854983-39855002 MS.gene37066:intron 25.0%
! TCATAAATGTCTTATCATAG+TGG + chr1.4:39855429-39855448 MS.gene37066:intron 25.0%
! TGTTAGTAGAATAATGAACA+AGG - chr1.4:39856106-39856125 None:intergenic 25.0%
! TTAGGTATACATATTGCTTT+GGG - chr1.4:39855044-39855063 None:intergenic 25.0%
! TTATATAAACCATACACTTC+TGG + chr1.4:39856588-39856607 MS.gene37066:intron 25.0%
! TTTAGGTATACATATTGCTT+TGG - chr1.4:39855045-39855064 None:intergenic 25.0%
!! ATCCATTGACTAATTAACAA+TGG - chr1.4:39854625-39854644 None:intergenic 25.0%
!! TTTCTGAAAGGAAATTTCAT+GGG - chr1.4:39854535-39854554 None:intergenic 25.0%
!!! AACAATGCATTTTAATATGC+AGG - chr1.4:39856476-39856495 None:intergenic 25.0%
!!! CGGTTCAATTAATTTTAAGT+CGG + chr1.4:39855204-39855223 MS.gene37066:intron 25.0%
!!! GATTTTAAGATAACTGCAAA+GGG + chr1.4:39855521-39855540 MS.gene37066:CDS 25.0%
!!! GTAGTTTTTGAAGGAATAAA+AGG + chr1.4:39855333-39855352 MS.gene37066:intron 25.0%
!!! TGATTTTAAGATAACTGCAA+AGG + chr1.4:39855520-39855539 MS.gene37066:CDS 25.0%
!!! TTTTCTGAAAGGAAATTTCA+TGG - chr1.4:39854536-39854555 None:intergenic 25.0%
AACTTCTAACACACAAGTTA+AGG - chr1.4:39854723-39854742 None:intergenic 30.0%
AAGGCTATACTACAAATGAT+GGG + chr1.4:39855723-39855742 MS.gene37066:CDS 30.0%
AATTTCCTTTCAGAAAACTC+AGG + chr1.4:39854539-39854558 MS.gene37066:CDS 30.0%
ACAAAAGAAAAGAACCAAAC+AGG + chr1.4:39857096-39857115 MS.gene37066:CDS 30.0%
AGGGTAAAAGAAGATTAAGA+TGG + chr1.4:39855540-39855559 MS.gene37066:CDS 30.0%
ATCATTTGTAGTATAGCCTT+AGG - chr1.4:39855723-39855742 None:intergenic 30.0%
ATGAGTTTGACATACCTATT+TGG + chr1.4:39854268-39854287 MS.gene37066:CDS 30.0%
ATTATTATTGTCAACCAGCT+TGG + chr1.4:39855587-39855606 MS.gene37066:CDS 30.0%
GAAAATTCATTGCTCCAAAT+AGG - chr1.4:39854285-39854304 None:intergenic 30.0%
GAAACTTCTAAATGGATCAT+TGG - chr1.4:39855370-39855389 None:intergenic 30.0%
GATTAAATTAAGCACTAGCT+AGG - chr1.4:39855679-39855698 None:intergenic 30.0%
GTAATTCACTTTAATCTGTG+TGG + chr1.4:39856080-39856099 MS.gene37066:intron 30.0%
GTGATTCTTCTATTTGCATT+TGG - chr1.4:39856830-39856849 None:intergenic 30.0%
TAAGGCTATACTACAAATGA+TGG + chr1.4:39855722-39855741 MS.gene37066:CDS 30.0%
TATCTTAAAATCAGAGTTGC+TGG - chr1.4:39855513-39855532 None:intergenic 30.0%
! AAAGGTTTCATTTGCTACAA+GGG - chr1.4:39854217-39854236 None:intergenic 30.0%
! ATATGCATGTACCTATTTTG+AGG + chr1.4:39855821-39855840 MS.gene37066:intron 30.0%
! ATGACTTTTGACATGAGAAA+TGG - chr1.4:39855763-39855782 None:intergenic 30.0%
! TAAAGGTTTCATTTGCTACA+AGG - chr1.4:39854218-39854237 None:intergenic 30.0%
! TCACCTTTTATGTATTGTCA+AGG + chr1.4:39854324-39854343 MS.gene37066:CDS 30.0%
! TGCCAATGCACATAATTTTA+AGG - chr1.4:39856745-39856764 None:intergenic 30.0%
! TTCTTTGTTTGTCAAAGTTG+CGG + chr1.4:39854925-39854944 MS.gene37066:intron 30.0%
! TTTAGAGCAATTTCAACAGT+GGG - chr1.4:39855121-39855140 None:intergenic 30.0%
!! TATGCATGTACCTATTTTGA+GGG + chr1.4:39855822-39855841 MS.gene37066:intron 30.0%
!! TTGTTGGACATCTTTTAAAG+CGG + chr1.4:39855184-39855203 MS.gene37066:intron 30.0%
!!! CTATCGTTTGATTTTTTCAG+CGG - chr1.4:39855268-39855287 None:intergenic 30.0%
!!! TTTTAGAGCAATTTCAACAG+TGG - chr1.4:39855122-39855141 None:intergenic 30.0%
AAACTATTCCAGCAATCGTT+CGG + chr1.4:39855139-39855158 MS.gene37066:intron 35.0%
AAAGGATGGAGTACGAAATT+TGG - chr1.4:39856803-39856822 None:intergenic 35.0%
AAAGGTGAATCTGTTGTTGT+TGG - chr1.4:39854312-39854331 None:intergenic 35.0%
AAGGTTTGCAAAGTAGAGAA+GGG + chr1.4:39856338-39856357 MS.gene37066:intron 35.0%
ACAAACAAAGAACTCGAGTT+TGG - chr1.4:39854917-39854936 None:intergenic 35.0%
ACCCTCTTATTAGAACAAGA+GGG - chr1.4:39854480-39854499 None:intergenic 35.0%
AGACATTTATGAGGCAGTTT+AGG - chr1.4:39855421-39855440 None:intergenic 35.0%
CAGCCTTGACAATACATAAA+AGG - chr1.4:39854330-39854349 None:intergenic 35.0%
CTATTTGCATTTGGCTACAA+AGG - chr1.4:39856821-39856840 None:intergenic 35.0%
TAGAGAAGGGAAAATGTGAA+AGG + chr1.4:39856315-39856334 MS.gene37066:intron 35.0%
TATAAAGCACAAACACTCCT+CGG + chr1.4:39855850-39855869 MS.gene37066:intron 35.0%
TATGTTGAGTGAATTCAGTG+TGG - chr1.4:39856264-39856283 None:intergenic 35.0%
TCATGTCAAAAGTCATTTGC+AGG + chr1.4:39855767-39855786 MS.gene37066:CDS 35.0%
TCCCTCTTGTTCTAATAAGA+GGG + chr1.4:39854476-39854495 MS.gene37066:CDS 35.0%
TCTATATCATGTTCCGCATT+GGG - chr1.4:39854684-39854703 None:intergenic 35.0%
TTCCCTCTTGTTCTAATAAG+AGG + chr1.4:39854475-39854494 MS.gene37066:CDS 35.0%
TTCTATATCATGTTCCGCAT+TGG - chr1.4:39854685-39854704 None:intergenic 35.0%
TTGTGTTTCAGGAATATCTG+AGG + chr1.4:39856653-39856672 MS.gene37066:intron 35.0%
TTTCAGGAATATCTGAGGAA+GGG + chr1.4:39856658-39856677 MS.gene37066:intron 35.0%
! AATCACCTGAGTTTTCTGAA+AGG - chr1.4:39854547-39854566 None:intergenic 35.0%
! AGCTCTGATGTTAGATATGT+CGG + chr1.4:39854802-39854821 MS.gene37066:intron 35.0%
! CATATTGCTTTGGGTACAAA+TGG - chr1.4:39855035-39855054 None:intergenic 35.0%
! GTGAGAAGAGTAGTTTTTGA+AGG + chr1.4:39855324-39855343 MS.gene37066:intron 35.0%
! TACTTACTACCAGAAGTGTA+TGG - chr1.4:39856600-39856619 None:intergenic 35.0%
!! TATACTACAAATGATGGGTG+TGG + chr1.4:39855728-39855747 MS.gene37066:CDS 35.0%
AACTACAACTTGAGGTTGAG+AGG + chr1.4:39856853-39856872 MS.gene37066:CDS 40.0%
ACCAACAACCTGCTTCAAAT+TGG + chr1.4:39854389-39854408 MS.gene37066:CDS 40.0%
AGAGGACTAACTATGCTACA+AGG - chr1.4:39854171-39854190 None:intergenic 40.0%
ATCACGACAACTACAACTTG+AGG + chr1.4:39856845-39856864 MS.gene37066:CDS 40.0%
ATTATTGTCAACCAGCTTGG+TGG + chr1.4:39855590-39855609 MS.gene37066:CDS 40.0%
CACACGCACAAATGTTTGAA+AGG + chr1.4:39856058-39856077 MS.gene37066:CDS 40.0%
CACCCTCTTATTAGAACAAG+AGG - chr1.4:39854481-39854500 None:intergenic 40.0%
CCAACAACCTGCTTCAAATT+GGG + chr1.4:39854390-39854409 MS.gene37066:CDS 40.0%
CCAATTGAACTATACTCGAG+AGG + chr1.4:39856525-39856544 MS.gene37066:intron 40.0%
CCTCTCGAGTATAGTTCAAT+TGG - chr1.4:39856528-39856547 None:intergenic 40.0%
CGCATTGGGATACATCTATA+CGG - chr1.4:39854670-39854689 None:intergenic 40.0%
CGTATAGATGTATCCCAATG+CGG + chr1.4:39854668-39854687 MS.gene37066:intron 40.0%
GAAGGGAAAATGTGAAAGGA+AGG + chr1.4:39856319-39856338 MS.gene37066:intron 40.0%
GAAGGTTTGCAAAGTAGAGA+AGG + chr1.4:39856337-39856356 MS.gene37066:intron 40.0%
GACAAACAATCTTCACCTTG+TGG + chr1.4:39855470-39855489 MS.gene37066:CDS 40.0%
GAGCAATAAACGACAATTCG+AGG + chr1.4:39856875-39856894 MS.gene37066:CDS 40.0%
GAGGACTAACTATGCTACAA+GGG - chr1.4:39854170-39854189 None:intergenic 40.0%
GTATGTCAAACTCATGCTGA+TGG - chr1.4:39854263-39854282 None:intergenic 40.0%
GTTGCAAAATCAAAGCCACA+AGG - chr1.4:39855488-39855507 None:intergenic 40.0%
GTTTCAGGAATATCTGAGGA+AGG + chr1.4:39856657-39856676 MS.gene37066:intron 40.0%
TAGCATAGTTAGTCCTCTAG+AGG + chr1.4:39854173-39854192 MS.gene37066:CDS 40.0%
TAGCGGGAGTTAATTGTTGT+TGG + chr1.4:39855168-39855187 MS.gene37066:intron 40.0%
TTAATCAGAGGCAAAGCCTA+AGG + chr1.4:39855704-39855723 MS.gene37066:intron 40.0%
TTGCATTTGGCTACAAAGGA+TGG - chr1.4:39856817-39856836 None:intergenic 40.0%
TTGTTTGTCAAAGTTGCGGT+GGG + chr1.4:39854929-39854948 MS.gene37066:intron 40.0%
TTTGTTTGTCAAAGTTGCGG+TGG + chr1.4:39854928-39854947 MS.gene37066:intron 40.0%
! GAGTTTGGTTTCACTCGATA+AGG - chr1.4:39854902-39854921 None:intergenic 40.0%
! GGTTGTTGGTTGATCTTGTT+TGG - chr1.4:39854379-39854398 None:intergenic 40.0%
! TTCAGAACCCAATTTGAAGC+AGG - chr1.4:39854400-39854419 None:intergenic 40.0%
! TTGTTCTAATAAGAGGGTGC+TGG + chr1.4:39854482-39854501 MS.gene37066:CDS 40.0%
! TTTGGTTTCACTCGATAAGG+AGG - chr1.4:39854899-39854918 None:intergenic 40.0%
!! ATCGGTCATTTTCTCCTGTT+TGG - chr1.4:39857113-39857132 None:intergenic 40.0%
!! TGCATTTTAATATGCAGGCG+CGG - chr1.4:39856471-39856490 None:intergenic 40.0%
AGGAAGGGCAGATAACAGAT+GGG + chr1.4:39856673-39856692 MS.gene37066:CDS 45.0%
ATTTCATGGGACCTCTGATC+TGG - chr1.4:39854522-39854541 None:intergenic 45.0%
GATGCAGCGAAATTTGCAAC+TGG + chr1.4:39857030-39857049 MS.gene37066:intron 45.0%
GATTAGACGTGTCCTGGTAT+CGG + chr1.4:39855872-39855891 MS.gene37066:intron 45.0%
GCATCTGATATGTCCTCTAG+AGG - chr1.4:39854189-39854208 None:intergenic 45.0%
TAGAAAAGCCGAACGATTGC+TGG - chr1.4:39855150-39855169 None:intergenic 45.0%
TTCACTCGATAAGGAGGTTG+AGG - chr1.4:39854893-39854912 None:intergenic 45.0%
TTTCATGGGACCTCTGATCT+GGG - chr1.4:39854521-39854540 None:intergenic 45.0%
! CAATCGTTCGGCTTTTCTAG+CGG + chr1.4:39855151-39855170 MS.gene37066:intron 45.0%
! CCCAATTTGAAGCAGGTTGT+TGG - chr1.4:39854393-39854412 None:intergenic 45.0%
! GTGATTTTTCCCCAGATCAG+AGG + chr1.4:39854508-39854527 MS.gene37066:CDS 45.0%
!! AATCGTTCGGCTTTTCTAGC+GGG + chr1.4:39855152-39855171 MS.gene37066:intron 45.0%
!! CATTTTAATATGCAGGCGCG+GGG - chr1.4:39856469-39856488 None:intergenic 45.0%
!! GCATTTTAATATGCAGGCGC+GGG - chr1.4:39856470-39856489 None:intergenic 45.0%
!! TATATGTGTCGGTGTCGTGT+CGG - chr1.4:39855917-39855936 None:intergenic 45.0%
!! TATTATATCATAAAAATAAA+TGG + chr1.4:39854776-39854795 MS.gene37066:intron 5.0%
GAGGAAGGGCAGATAACAGA+TGG + chr1.4:39856672-39856691 MS.gene37066:CDS 50.0%
TACGACATGTGTCCGATACC+AGG - chr1.4:39855887-39855906 None:intergenic 50.0%
TTCATGGGACCTCTGATCTG+GGG - chr1.4:39854520-39854539 None:intergenic 50.0%
ACCAGGACACGTCTAATCCG+AGG - chr1.4:39855870-39855889 None:intergenic 55.0%
TACTTTCTGGGCCACCAAGC+TGG - chr1.4:39855604-39855623 None:intergenic 55.0%
! TCCTCGGATTAGACGTGTCC+TGG + chr1.4:39855866-39855885 MS.gene37066:intron 55.0%
CAGGCGCGGGGTTCAAACTT+TGG - chr1.4:39856457-39856476 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 39854126 39857147 39854126 ID=MS.gene37066
chr1.4 mRNA 39854126 39857147 39854126 ID=MS.gene37066.t1;Parent=MS.gene37066
chr1.4 exon 39854126 39854560 39854126 ID=MS.gene37066.t1.exon1;Parent=MS.gene37066.t1
chr1.4 CDS 39854126 39854560 39854126 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39855449 39855620 39855449 ID=MS.gene37066.t1.exon2;Parent=MS.gene37066.t1
chr1.4 CDS 39855449 39855620 39855449 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39855712 39855788 39855712 ID=MS.gene37066.t1.exon3;Parent=MS.gene37066.t1
chr1.4 CDS 39855712 39855788 39855712 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39856040 39856079 39856040 ID=MS.gene37066.t1.exon4;Parent=MS.gene37066.t1
chr1.4 CDS 39856040 39856079 39856040 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39856664 39856718 39856664 ID=MS.gene37066.t1.exon5;Parent=MS.gene37066.t1
chr1.4 CDS 39856664 39856718 39856664 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39856827 39856896 39856827 ID=MS.gene37066.t1.exon6;Parent=MS.gene37066.t1
chr1.4 CDS 39856827 39856896 39856827 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
chr1.4 exon 39857031 39857147 39857031 ID=MS.gene37066.t1.exon7;Parent=MS.gene37066.t1
chr1.4 CDS 39857031 39857147 39857031 ID=cds.MS.gene37066.t1;Parent=MS.gene37066.t1
Gene Sequence

>MS.gene37066

ATGTACATAAATCATTGGGATTATGAACATCAAAGCTATGACCCTTGTAGCATAGTTAGTCCTCTAGAGGACATATCAGATGCTATGACCCTTGTAGCAAATGAAACCTTTATGAATTTTCAAGATCAACAACACCATCAGCATGAGTTTGACATACCTATTTGGAGCAATGAATTTTCTATGCCAACAACAACAGATTCACCTTTTATGTATTGTCAAGGCTGTAAAGATAACTACACTGAAAATATCACCAAACAAGATCAACCAACAACCTGCTTCAAATTGGGTTCTGAATCTTTAACGTCTAGCAGTGAAGATAGTATTTCTTCTTGTGAAAAGTGTAGTGAGTTTCCCTCTTGTTCTAATAAGAGGGTGCTGGAGAGTGATTTTTCCCCAGATCAGAGGTCCCATGAAATTTCCTTTCAGAAAACTCAGGTGATTTTTTTATTTTTTTAACTACCTTTCTTATTACTCTTCAAATTTTTGTTGGATCAAACCATTGTTAATTAGTCAATGGATGAATTTAGGAACACATTTGTCACCGTATAGATGTATCCCAATGCGGAACATGATATAGAATTTCTATTTTTGTTTCCTTAACTTGTGTGTTAGAAGTTAGAACACTTGTTAGTAATAAGACTAAGTTGTTATATTATATCATAAAAATAAATGGTAGAGCTCTGATGTTAGATATGTCGGCATAATTTGTCGAACCTCTTTAACAAATAATTGTTTCATTATTTTTTGACAAATATCACTTCACTCCTCAACCTCCTTATCGAGTGAAACCAAACTCGAGTTCTTTGTTTGTCAAAGTTGCGGTGGGAGTTCCTTAATTTCTATATGACTCTTGAAATTAATTGACTACAGTTTCAATAGGAATTATTGATTACAATGAATTTGGGTCCATTTGTACCCAAAGCAATATGTATACCTAAAAGGGTATCATTTTATTAGTTTTCTTTTAAAATAATTTTTGAGAAATTACTCTTCCCACTGTTGAAATTGCTCTAAAACTATTCCAGCAATCGTTCGGCTTTTCTAGCGGGAGTTAATTGTTGTTGGACATCTTTTAAAGCGGTTCAATTAATTTTAAGTCGGCTTAACCAATCTTAAATATGTATGATGACGGTTCACTGCCGCTGAAAAAATCAAACGATAGTTCTGTTGAAAAAATAGTTTTTGAACAATTTTAAAAGTGAGAAGAGTAGTTTTTGAAGGAATAAAAGGAAATTTTGATCCCAATGATCCATTTAGAAGTTTCTATGTAACAAACATTCATTTTATTTTATCCTAAACTGCCTCATAAATGTCTTATCATAGTGGGAATCATGTACAAAGCAAGACAAACAATCTTCACCTTGTGGCTTTGATTTTGCAACATCCAGCAACTCTGATTTTAAGATAACTGCAAAGGGTAAAAGAAGATTAAGATGGACAAAAGAATTGAATGAGTCATTTATTATTATTGTCAACCAGCTTGGTGGCCCAGAAAGTAAGTTTATATAATTCATTTTATAGGTAACAAAATAGTTTGAAATTGCATGTTACCTAGCTAGTGCTTAATTTAATCATTGATTAATCAGAGGCAAAGCCTAAGGCTATACTACAAATGATGGGTGTGGATGTGTTGACCATTTCTCATGTCAAAAGTCATTTGCAGGTCAGTTTATTTTCATGAGACAATTGTTATATATATGCATGTACCTATTTTGAGGGTAAATTATAAAGCACAAACACTCCTCGGATTAGACGTGTCCTGGTATCGGACACATGTCGTATCTAACACCGACACGACACCGACACATATAATTATACTTAATTATGTGATTTTAATAATTTATTAGCGATATGGGCGAGTTACTGCAAACTACTAAGTTGCTTTCTAATATTTCTATTATAATTTTCATGCAGAAATATCGTTCTACATTACACACGCACAAATGTTTGAAAGGTAATTCACTTTAATCTGTGTGGCCTTGTTCATTATTCTACTAACAATAATAGTCCTTTATGAAATGCATTTTAACCAAATTTAGTTTTCAATTTCAATTCTGCGATTTGATTTTCAGTTGAGTTTCAATTATGAATTAAGTTTCTGTTTTTATTTTTGAGTTAAGTCACTATTTACTTCACCACACTGAATTCACTCAACATAAGAAAATATTGACAAAAAAATTAATTAAAAATAGAGAAGGGAAAATGTGAAAGGAAGGTTTGCAAAGTAGAGAAGGGAAAATTGATATACTGTAGACACCATGAATCTTTTTTTTTTTTTTTTTTTTTTTCAAATATTAGTGGCTAGAAATTCATGAATAAGTAGGGAGTCCAAAGTTTGAACCCCGCGCCTGCATATTAAAATGCATTGTTTTTCTTATTAATAAAATGAATTGTTCTTACCAATTGAACTATACTCGAGAGGACAAACTATATTTACTTATATAAAAAAAAACAAATTTTACTTATATAAACCATACACTTCTGGTAGTAAGTACTATCACCTTAACCTCCAATATATATTAATTTATTGTGTTTCAGGAATATCTGAGGAAGGGCAGATAACAGATGGGATTAATGAGCTTCAAGTTAAAATGTAACATCATTTAGCTATGTTAACCTTAAAATTATGTGCATTGGCACTACAATGTCGATGCAAAATGTATAAACGTGTTTACCAAATTTCGTACTCCATCCTTTGTAGCCAAATGCAAATAGAAGAATCACGACAACTACAACTTGAGGTTGAGAGGAGCAATAAACGACAATTCGAGGTATATATACTCTAATTATTTACATCAAGAATTCAGGAAACACTGTATGTTTTTAATTATTCTACATTATATTTTATATCAAGAATTAAGGAAATATTATATGTTTTTGATTATTTATTTTGTCTTGTGGTCAGATGCAGCGAAATTTGCAACTGGTGATTGAACAGCAGAAGAAGCAGCTCAAGTTAATGTTAGATCAACAAAAGAAAAGAACCAAACAGGAGAAAATGACCGATTTAAGACGAAAATGA
Protein sequence

>MS.gene37066.t1

MYINHWDYEHQSYDPCSIVSPLEDISDAMTLVANETFMNFQDQQHHQHEFDIPIWSNEFSMPTTTDSPFMYCQGCKDNYTENITKQDQPTTCFKLGSESLTSSSEDSISSCEKCSEFPSCSNKRVLESDFSPDQRSHEISFQKTQWESCTKQDKQSSPCGFDFATSSNSDFKITAKGKRRLRWTKELNESFIIIVNQLGGPEKAKPKAILQMMGVDVLTISHVKSHLQKYRSTLHTHKCLKGISEEGQITDGINELQVKIQMQIEESRQLQLEVERSNKRQFEMQRNLQLVIEQQKKQLKLMLDQQKKRTKQEKMTDLRRK