Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37210.t1 | XP_013451230.1 | 92 | 88 | 7 | 0 | 1 | 88 | 1 | 88 | 1.90E-36 | 161.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37210.t1 | Q5N6V8 | 35.8 | 81 | 51 | 1 | 9 | 88 | 6 | 86 | 5.5e-08 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37210.t1 | A0A072UHJ5 | 92.0 | 88 | 7 | 0 | 1 | 88 | 1 | 88 | 1.4e-36 | 161.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37210.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37210.t1 | MTR_6g016850 | 92.045 | 88 | 7 | 0 | 1 | 88 | 1 | 88 | 4.01e-54 | 166 |
| MS.gene37210.t1 | MTR_8g105600 | 88.636 | 88 | 10 | 0 | 1 | 88 | 1 | 88 | 1.88e-47 | 159 |
| MS.gene37210.t1 | MTR_4g021790 | 51.685 | 89 | 42 | 1 | 1 | 88 | 1 | 89 | 2.14e-25 | 95.5 |
| MS.gene37210.t1 | MTR_4g021845 | 45.349 | 86 | 47 | 0 | 3 | 88 | 4 | 89 | 4.30e-22 | 85.9 |
| MS.gene37210.t1 | MTR_4g021760 | 48.750 | 80 | 41 | 0 | 9 | 88 | 11 | 90 | 4.40e-22 | 87.4 |
| MS.gene37210.t1 | MTR_5g055260 | 47.500 | 80 | 42 | 0 | 9 | 88 | 10 | 89 | 1.08e-21 | 85.5 |
| MS.gene37210.t1 | MTR_8g093040 | 51.163 | 86 | 41 | 1 | 4 | 88 | 9 | 94 | 2.19e-21 | 84.7 |
| MS.gene37210.t1 | MTR_4g021855 | 46.250 | 80 | 43 | 0 | 9 | 88 | 10 | 89 | 1.12e-20 | 83.6 |
| MS.gene37210.t1 | MTR_6g045327 | 50.000 | 82 | 40 | 1 | 8 | 88 | 13 | 94 | 1.10e-18 | 79.0 |
| MS.gene37210.t1 | MTR_1g032570 | 39.759 | 83 | 49 | 1 | 7 | 88 | 9 | 91 | 7.58e-15 | 68.2 |
| MS.gene37210.t1 | MTR_3g086100 | 37.805 | 82 | 50 | 1 | 8 | 88 | 12 | 93 | 8.87e-14 | 65.1 |
| MS.gene37210.t1 | MTR_4g121020 | 36.145 | 83 | 52 | 1 | 7 | 88 | 29 | 111 | 2.62e-12 | 60.8 |
| MS.gene37210.t1 | MTR_4g121020 | 36.145 | 83 | 52 | 1 | 7 | 88 | 29 | 111 | 2.62e-12 | 60.8 |
| MS.gene37210.t1 | MTR_4g131600 | 38.554 | 83 | 50 | 1 | 5 | 86 | 16 | 98 | 3.28e-12 | 60.8 |
| MS.gene37210.t1 | MTR_8g079940 | 35.802 | 81 | 51 | 1 | 9 | 88 | 25 | 105 | 1.05e-11 | 59.3 |
| MS.gene37210.t1 | MTR_7g117705 | 34.524 | 84 | 53 | 1 | 7 | 88 | 3 | 86 | 2.52e-11 | 58.2 |
| MS.gene37210.t1 | MTR_2g034960 | 36.145 | 83 | 52 | 1 | 7 | 88 | 27 | 109 | 3.73e-11 | 57.8 |
| MS.gene37210.t1 | MTR_1g013180 | 34.940 | 83 | 53 | 1 | 7 | 88 | 154 | 236 | 4.93e-11 | 57.4 |
| MS.gene37210.t1 | MTR_1g013170 | 36.585 | 82 | 51 | 1 | 8 | 88 | 4 | 85 | 5.02e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37210.t1 | AT1G67710 | 36.364 | 77 | 48 | 1 | 13 | 88 | 11 | 87 | 8.11e-12 | 59.7 |
| MS.gene37210.t1 | AT2G01760 | 35.714 | 84 | 52 | 2 | 7 | 88 | 5 | 88 | 5.72e-11 | 57.0 |
Find 0 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGTTAATATAATTCAATTA+AGG | + | 42583:1445-1464 | MS.gene37210:intergenic | 15.0% |
| ! | AAAAAGATTGAGACTTTCAT+AGG | + | 42583:1465-1484 | MS.gene37210:intergenic | 25.0% |
| ! | AATCACAAGAACTTTAACAT+TGG | + | 42583:1352-1371 | MS.gene37210:intergenic | 25.0% |
| AAATCAAATGAATCCATGTC+CGG | + | 42583:1601-1620 | MS.gene37209:intergenic | 30.0% | |
| CAATTAAGGATTACAAACCT+TGG | + | 42583:1431-1450 | MS.gene37210:intergenic | 30.0% | |
| TCAATGCTATTGAGAAAACA+TGG | - | 42583:1391-1410 | MS.gene37210:CDS | 30.0% | |
| ! | CTTGAATTTGATAGCACAAA+AGG | - | 42583:1540-1559 | MS.gene37210:intron | 30.0% |
| ! | CTTTTGTGCTATCAAATTCA+AGG | + | 42583:1542-1561 | MS.gene37210:intergenic | 30.0% |
| !!! | ACAAAAGGTGCATTTTGATT+TGG | - | 42583:1555-1574 | MS.gene37210:CDS | 30.0% |
| ACTTCTTGAAACTCAAATGC+CGG | - | 42583:1579-1598 | MS.gene37210:CDS | 35.0% | |
| TGGTCTCAATTCAATTACCA+AGG | - | 42583:1411-1430 | MS.gene37210:CDS | 35.0% | |
| GGCTCGAAACTCATTTCCAA+AGG | + | 42583:1331-1350 | MS.gene37210:intergenic | 45.0% | |
| TGAAACTCAAATGCCGGACA+TGG | - | 42583:1585-1604 | MS.gene37210:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42583 | gene | 1318 | 1661 | 1318 | ID=MS.gene37210 |
| 42583 | mRNA | 1318 | 1661 | 1318 | ID=MS.gene37210.t1;Parent=MS.gene37210 |
| 42583 | exon | 1547 | 1661 | 1547 | ID=MS.gene37210.t1.exon1;Parent=MS.gene37210.t1 |
| 42583 | CDS | 1547 | 1661 | 1547 | ID=cds.MS.gene37210.t1;Parent=MS.gene37210.t1 |
| 42583 | exon | 1318 | 1467 | 1318 | ID=MS.gene37210.t1.exon2;Parent=MS.gene37210.t1 |
| 42583 | CDS | 1318 | 1467 | 1318 | ID=cds.MS.gene37210.t1;Parent=MS.gene37210.t1 |
| Gene Sequence |
| Protein sequence |
