Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37482.t1 | RHN70377.1 | 75.8 | 458 | 41 | 3 | 86 | 543 | 85 | 472 | 4.90E-191 | 677.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37482.t1 | F4IXE7 | 31.0 | 455 | 256 | 12 | 105 | 542 | 617 | 1030 | 1.0e-51 | 206.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37482.t1 | A0A396J566 | 75.8 | 458 | 41 | 3 | 86 | 543 | 85 | 472 | 3.6e-191 | 677.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37482.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37482.t1 | MTR_7g109640 | 51.166 | 643 | 177 | 12 | 4 | 536 | 173 | 788 | 0.0 | 559 |
| MS.gene37482.t1 | MTR_3g103120 | 57.319 | 567 | 185 | 10 | 4 | 537 | 292 | 834 | 0.0 | 546 |
| MS.gene37482.t1 | MTR_3g103110 | 48.827 | 682 | 183 | 12 | 3 | 537 | 131 | 793 | 0.0 | 540 |
| MS.gene37482.t1 | MTR_5g024390 | 56.948 | 439 | 157 | 9 | 108 | 535 | 2 | 419 | 1.01e-163 | 472 |
| MS.gene37482.t1 | MTR_5g024500 | 56.264 | 439 | 160 | 9 | 108 | 535 | 2 | 419 | 7.22e-161 | 465 |
| MS.gene37482.t1 | MTR_3g103160 | 84.740 | 308 | 25 | 3 | 1 | 308 | 1 | 286 | 2.86e-142 | 418 |
| MS.gene37482.t1 | MTR_3g103160 | 91.176 | 170 | 15 | 0 | 374 | 543 | 275 | 444 | 3.80e-103 | 318 |
| MS.gene37482.t1 | MTR_5g071340 | 36.481 | 540 | 272 | 15 | 16 | 537 | 600 | 1086 | 6.35e-92 | 305 |
| MS.gene37482.t1 | MTR_3g464550 | 37.030 | 505 | 268 | 12 | 45 | 535 | 540 | 1008 | 4.71e-87 | 291 |
| MS.gene37482.t1 | MTR_2g078040 | 34.234 | 444 | 244 | 10 | 105 | 535 | 758 | 1166 | 1.08e-67 | 238 |
| MS.gene37482.t1 | MTR_1g072130 | 32.584 | 445 | 268 | 12 | 105 | 542 | 808 | 1227 | 1.93e-61 | 219 |
| MS.gene37482.t1 | MTR_1g103830 | 33.976 | 415 | 234 | 7 | 105 | 509 | 602 | 986 | 2.52e-61 | 219 |
| MS.gene37482.t1 | MTR_1g068930 | 26.667 | 435 | 218 | 14 | 143 | 523 | 459 | 846 | 8.12e-39 | 152 |
| MS.gene37482.t1 | MTR_1g068930 | 26.667 | 435 | 218 | 14 | 143 | 523 | 496 | 883 | 1.01e-38 | 152 |
| MS.gene37482.t1 | MTR_7g095140 | 30.921 | 304 | 169 | 9 | 222 | 516 | 13 | 284 | 1.04e-37 | 143 |
| MS.gene37482.t1 | MTR_4g099530 | 27.250 | 400 | 239 | 12 | 137 | 529 | 138 | 492 | 1.41e-32 | 131 |
| MS.gene37482.t1 | MTR_4g056990 | 24.838 | 463 | 252 | 13 | 107 | 520 | 188 | 603 | 8.45e-32 | 130 |
| MS.gene37482.t1 | MTR_1g062330 | 25.495 | 455 | 229 | 15 | 143 | 530 | 386 | 797 | 9.89e-32 | 130 |
| MS.gene37482.t1 | MTR_7g102610 | 25.373 | 469 | 230 | 17 | 135 | 533 | 527 | 945 | 2.76e-30 | 126 |
| MS.gene37482.t1 | MTR_4g050090 | 25.466 | 483 | 229 | 17 | 113 | 519 | 199 | 626 | 8.73e-30 | 124 |
| MS.gene37482.t1 | MTR_4g057290 | 24.249 | 466 | 236 | 13 | 113 | 508 | 94 | 512 | 1.10e-25 | 111 |
| MS.gene37482.t1 | MTR_6g061000 | 50.000 | 80 | 37 | 1 | 198 | 277 | 25 | 101 | 1.08e-17 | 79.0 |
| MS.gene37482.t1 | MTR_1g062330 | 24.507 | 355 | 160 | 14 | 143 | 432 | 386 | 697 | 1.22e-17 | 87.0 |
| MS.gene37482.t1 | MTR_2g084425 | 25.000 | 192 | 135 | 4 | 347 | 535 | 833 | 1018 | 4.46e-15 | 79.0 |
| MS.gene37482.t1 | MTR_2g084425 | 25.513 | 341 | 154 | 12 | 61 | 347 | 416 | 710 | 4.13e-13 | 72.8 |
| MS.gene37482.t1 | MTR_2g084425 | 30.093 | 216 | 107 | 7 | 61 | 268 | 416 | 595 | 2.55e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 314 | 783 | 7.24e-84 | 278 |
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 545 | 1014 | 1.31e-82 | 278 |
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 545 | 1014 | 1.31e-82 | 278 |
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 545 | 1014 | 1.67e-82 | 278 |
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 536 | 1005 | 2.03e-82 | 278 |
| MS.gene37482.t1 | AT5G58610 | 35.729 | 501 | 266 | 11 | 66 | 541 | 536 | 1005 | 2.29e-82 | 278 |
| MS.gene37482.t1 | AT5G58610 | 34.608 | 497 | 251 | 12 | 66 | 541 | 536 | 979 | 4.40e-75 | 258 |
| MS.gene37482.t1 | AT5G58610 | 34.608 | 497 | 251 | 12 | 66 | 541 | 536 | 979 | 7.15e-75 | 257 |
| MS.gene37482.t1 | AT5G58610 | 34.932 | 438 | 229 | 11 | 66 | 478 | 545 | 951 | 1.24e-66 | 233 |
| MS.gene37482.t1 | AT5G58610 | 34.932 | 438 | 229 | 11 | 66 | 478 | 536 | 942 | 1.44e-66 | 233 |
| MS.gene37482.t1 | AT5G36740 | 30.853 | 551 | 331 | 13 | 8 | 541 | 438 | 955 | 1.08e-65 | 231 |
| MS.gene37482.t1 | AT5G36740 | 30.853 | 551 | 331 | 13 | 8 | 541 | 438 | 955 | 1.08e-65 | 231 |
| MS.gene37482.t1 | AT5G36740 | 30.411 | 559 | 333 | 14 | 8 | 541 | 285 | 812 | 9.71e-63 | 222 |
| MS.gene37482.t1 | AT3G14980 | 30.966 | 507 | 279 | 16 | 50 | 535 | 522 | 978 | 5.10e-59 | 212 |
| MS.gene37482.t1 | AT3G14980 | 30.966 | 507 | 279 | 16 | 50 | 535 | 567 | 1023 | 5.50e-59 | 212 |
| MS.gene37482.t1 | AT3G14980 | 30.966 | 507 | 279 | 16 | 50 | 535 | 567 | 1023 | 5.50e-59 | 212 |
| MS.gene37482.t1 | AT3G14980 | 30.966 | 507 | 279 | 16 | 50 | 535 | 567 | 1023 | 5.50e-59 | 212 |
| MS.gene37482.t1 | AT3G14980 | 30.966 | 507 | 279 | 16 | 50 | 535 | 567 | 1023 | 7.79e-59 | 211 |
| MS.gene37482.t1 | AT1G05380 | 32.273 | 440 | 265 | 9 | 105 | 538 | 516 | 928 | 7.80e-59 | 211 |
| MS.gene37482.t1 | AT1G05380 | 32.273 | 440 | 265 | 9 | 105 | 538 | 516 | 928 | 7.80e-59 | 211 |
| MS.gene37482.t1 | AT1G05380 | 32.273 | 440 | 265 | 9 | 105 | 538 | 516 | 928 | 7.80e-59 | 211 |
| MS.gene37482.t1 | AT5G36670 | 28.998 | 569 | 336 | 15 | 6 | 541 | 436 | 969 | 8.18e-58 | 209 |
| MS.gene37482.t1 | AT5G36670 | 28.998 | 569 | 336 | 15 | 6 | 541 | 436 | 969 | 8.18e-58 | 209 |
| MS.gene37482.t1 | AT5G36740 | 31.084 | 489 | 287 | 13 | 6 | 477 | 436 | 891 | 6.66e-57 | 205 |
| MS.gene37482.t1 | AT5G36740 | 31.084 | 489 | 287 | 13 | 6 | 477 | 436 | 891 | 6.66e-57 | 205 |
| MS.gene37482.t1 | AT5G36670 | 31.084 | 489 | 287 | 13 | 6 | 477 | 436 | 891 | 6.66e-57 | 205 |
| MS.gene37482.t1 | AT5G36670 | 28.596 | 577 | 338 | 16 | 6 | 541 | 283 | 826 | 8.17e-55 | 199 |
| MS.gene37482.t1 | AT5G36670 | 28.596 | 577 | 338 | 16 | 6 | 541 | 436 | 979 | 2.58e-54 | 198 |
| MS.gene37482.t1 | AT5G58610 | 33.827 | 405 | 212 | 11 | 66 | 445 | 536 | 909 | 3.28e-54 | 197 |
| MS.gene37482.t1 | AT4G14920 | 28.707 | 526 | 296 | 17 | 14 | 525 | 513 | 973 | 3.50e-54 | 198 |
| MS.gene37482.t1 | AT4G14920 | 28.707 | 526 | 296 | 17 | 14 | 525 | 513 | 973 | 3.50e-54 | 198 |
| MS.gene37482.t1 | AT4G14920 | 28.707 | 526 | 296 | 17 | 14 | 525 | 513 | 973 | 4.07e-54 | 198 |
| MS.gene37482.t1 | AT5G63900 | 36.562 | 320 | 169 | 9 | 222 | 538 | 255 | 543 | 4.54e-54 | 192 |
| MS.gene37482.t1 | AT2G37520 | 27.483 | 433 | 227 | 14 | 143 | 524 | 410 | 806 | 2.61e-38 | 150 |
| MS.gene37482.t1 | AT3G53680 | 27.842 | 431 | 227 | 14 | 143 | 524 | 427 | 822 | 5.03e-38 | 149 |
| MS.gene37482.t1 | AT3G53680 | 27.842 | 431 | 227 | 14 | 143 | 524 | 429 | 824 | 5.54e-38 | 149 |
| MS.gene37482.t1 | AT3G14980 | 29.904 | 418 | 222 | 16 | 50 | 446 | 567 | 934 | 1.45e-37 | 148 |
| MS.gene37482.t1 | AT2G36720 | 24.138 | 464 | 244 | 11 | 143 | 540 | 557 | 978 | 2.63e-35 | 142 |
| MS.gene37482.t1 | AT2G36720 | 24.138 | 464 | 244 | 11 | 143 | 540 | 557 | 978 | 2.63e-35 | 142 |
| MS.gene37482.t1 | AT2G27980 | 25.957 | 470 | 236 | 13 | 143 | 542 | 637 | 1064 | 6.42e-31 | 128 |
Find 99 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATACTTTCTTTGAATTTC+AGG | 0.141944 | 3.4:+90747702 | MS.gene37482:CDS |
| ACATGTTCTGTTTGTTGTTT+TGG | 0.157762 | 3.4:+90746818 | MS.gene37482:CDS |
| AGATCATTCCCAAGCTTATT+TGG | 0.196412 | 3.4:-90747190 | None:intergenic |
| GTGGCAACCATAAGGATTTA+TGG | 0.239122 | 3.4:+90747466 | MS.gene37482:CDS |
| TGGTTTCTATACTGCGATTA+TGG | 0.240807 | 3.4:+90747420 | MS.gene37482:CDS |
| TGCAAATTCTTGTTTGCGTT+TGG | 0.242204 | 3.4:-90746972 | None:intergenic |
| ACCATCCAAAAGTATCTTCT+TGG | 0.296021 | 3.4:+90746649 | MS.gene37482:CDS |
| GTTGATAAAGGATGGAGTTT+TGG | 0.297915 | 3.4:+90746481 | MS.gene37482:CDS |
| GTCTTCCAAGAAGATACTTT+TGG | 0.305890 | 3.4:-90746654 | None:intergenic |
| ACAAGCATGAGCCTCAAATT+TGG | 0.327395 | 3.4:-90746612 | None:intergenic |
| TCGTTCCACTCTGATTGTTT+AGG | 0.327474 | 3.4:+90746875 | MS.gene37482:CDS |
| CTAAAGAGGGAAGGTATAAG+AGG | 0.337184 | 3.4:+90746446 | MS.gene37482:CDS |
| ACAAGAGATACTCCCTCTTT+TGG | 0.339052 | 3.4:-90746546 | None:intergenic |
| CGGAACTTCATTGTTGGACT+TGG | 0.361651 | 3.4:+90746676 | MS.gene37482:CDS |
| GTTTCTAAAGCCTCTAGTTC+CGG | 0.368804 | 3.4:+90746299 | MS.gene37482:CDS |
| GCAAATTCTTGTTTGCGTTT+GGG | 0.380503 | 3.4:-90746971 | None:intergenic |
| CAGACCATCCGGATCTCGTT+TGG | 0.386226 | 3.4:-90746900 | None:intergenic |
| CCAAGTGTCTATAGCATCCT+TGG | 0.387385 | 3.4:-90747623 | None:intergenic |
| GGAACGGATAAATCAACTTC+TGG | 0.396861 | 3.4:+90746272 | MS.gene37482:CDS |
| ACATGTTCCCACAAACTTCA+TGG | 0.397494 | 3.4:-90747138 | None:intergenic |
| ATTCACTCTCACCAAATTTG+AGG | 0.418669 | 3.4:+90746601 | MS.gene37482:CDS |
| TGTGTAAAGGCAACAAGAAT+TGG | 0.422575 | 3.4:+90747055 | MS.gene37482:CDS |
| CATAAGGATTTATGGTCAAA+AGG | 0.428606 | 3.4:+90747474 | MS.gene37482:CDS |
| CAAACGAGATCCGGATGGTC+TGG | 0.433972 | 3.4:+90746901 | MS.gene37482:CDS |
| AGTTGATTAACTTAGGAGTT+GGG | 0.439665 | 3.4:+90747581 | MS.gene37482:CDS |
| GAGACACTTTCTTTGTTAAG+TGG | 0.443344 | 3.4:-90747745 | None:intergenic |
| AGACCATCCGGATCTCGTTT+GGG | 0.445042 | 3.4:-90746899 | None:intergenic |
| AAGAAACGTTTGAACTTTCG+TGG | 0.445092 | 3.4:+90747400 | MS.gene37482:CDS |
| TTGTAATGCTGAAGCAAAAT+CGG | 0.448060 | 3.4:+90746745 | MS.gene37482:CDS |
| TTTAGGTCCCAAACGAGATC+CGG | 0.450042 | 3.4:+90746892 | MS.gene37482:CDS |
| ATGTGACTAGAAACTTCATT+TGG | 0.453838 | 3.4:-90747082 | None:intergenic |
| GGAAGACGGAACTTCATTGT+TGG | 0.455884 | 3.4:+90746670 | MS.gene37482:CDS |
| TCTGAGCTCAGCCAATCAAA+AGG | 0.457877 | 3.4:+90746715 | MS.gene37482:CDS |
| ATTGACAATGTTACCAAAAG+AGG | 0.459673 | 3.4:+90746533 | MS.gene37482:CDS |
| GAAGTGTTCTCAGTTGATAA+AGG | 0.461389 | 3.4:+90746469 | MS.gene37482:CDS |
| CGTAAATTGACTGTGGCTCT+TGG | 0.463118 | 3.4:+90747289 | MS.gene37482:CDS |
| GAGGAACAGTTGATTAACTT+AGG | 0.464976 | 3.4:+90747574 | MS.gene37482:CDS |
| GGTGTGCTGCGTGAGAGTTT+TGG | 0.469338 | 3.4:+90747310 | MS.gene37482:CDS |
| TGTTGTTGTTGTTCCTTCCT+CGG | 0.473116 | 3.4:-90746192 | None:intergenic |
| CTGAGCTCAGCCAATCAAAA+GGG | 0.474959 | 3.4:+90746716 | MS.gene37482:CDS |
| CTAGGAATACCAAATAAGCT+TGG | 0.478097 | 3.4:+90747181 | MS.gene37482:CDS |
| TATACTGCGATTATGGAGAA+AGG | 0.478537 | 3.4:+90747427 | MS.gene37482:CDS |
| CCGTTCCCTCAGATTCTCTC+AGG | 0.480436 | 3.4:-90746256 | None:intergenic |
| TAGGAATACCAAATAAGCTT+GGG | 0.480908 | 3.4:+90747182 | MS.gene37482:CDS |
| ATACTGCGATTATGGAGAAA+GGG | 0.484197 | 3.4:+90747428 | MS.gene37482:CDS |
| ATTTCACTTGGGGTGTGTAA+AGG | 0.484386 | 3.4:+90747042 | MS.gene37482:CDS |
| CAGTTGATTAACTTAGGAGT+TGG | 0.492298 | 3.4:+90747580 | MS.gene37482:CDS |
| TATAATGTGAGTTGTGATGA+TGG | 0.496882 | 3.4:+90747232 | MS.gene37482:CDS |
| AGCTCAGAGCTTTCATTCGA+CGG | 0.500191 | 3.4:-90746700 | None:intergenic |
| TAACTTAGGAGTTGGGAGCT+TGG | 0.501005 | 3.4:+90747588 | MS.gene37482:CDS |
| AGCTTGGGAATGATCTAACT+TGG | 0.501787 | 3.4:+90747197 | MS.gene37482:CDS |
| GGATAAATCAACTTCTGGTA+AGG | 0.504650 | 3.4:+90746277 | MS.gene37482:CDS |
| AAAGAGGGAAAGTACTAAAG+AGG | 0.506275 | 3.4:+90746432 | MS.gene37482:CDS |
| TGAGTTCTGTGGCAACCATA+AGG | 0.512428 | 3.4:+90747458 | MS.gene37482:CDS |
| CGGATGGTCTGGATGGTACA+TGG | 0.516273 | 3.4:+90746912 | MS.gene37482:CDS |
| AAGAGGGAAAGTACTAAAGA+GGG | 0.517147 | 3.4:+90746433 | MS.gene37482:CDS |
| AATGTGCCGTTTGTTGATGA+AGG | 0.520351 | 3.4:+90747546 | MS.gene37482:CDS |
| GTTTCTAATTCAGTGGGTAG+TGG | 0.524886 | 3.4:+90746407 | MS.gene37482:CDS |
| CCAAGGATGCTATAGACACT+TGG | 0.526403 | 3.4:+90747623 | MS.gene37482:CDS |
| TAAAGAGGGAAGGTATAAGA+GGG | 0.542556 | 3.4:+90746447 | MS.gene37482:CDS |
| CATGTACCATCCAGACCATC+CGG | 0.542947 | 3.4:-90746911 | None:intergenic |
| CAATCAGAGTGGAACGAAGA+AGG | 0.546313 | 3.4:-90746869 | None:intergenic |
| ATCAGAAGTTTCTAATTCAG+TGG | 0.547714 | 3.4:+90746400 | MS.gene37482:CDS |
| TCAGTGGGTAGTGGTAAAAG+AGG | 0.549198 | 3.4:+90746416 | MS.gene37482:CDS |
| ATGTGAACGAAAATTTCACT+TGG | 0.549257 | 3.4:+90747030 | MS.gene37482:CDS |
| TGTTCTCAGTTGATAAAGGA+TGG | 0.550925 | 3.4:+90746473 | MS.gene37482:CDS |
| CGAGATCCGGATGGTCTGGA+TGG | 0.554408 | 3.4:+90746905 | MS.gene37482:CDS |
| CAGTGGGTAGTGGTAAAAGA+GGG | 0.562756 | 3.4:+90746417 | MS.gene37482:CDS |
| CAAGCCCCTTCATCAACAAA+CGG | 0.563396 | 3.4:-90747552 | None:intergenic |
| AAAAGTATCTTCTTGGAAGA+CGG | 0.564085 | 3.4:+90746656 | MS.gene37482:CDS |
| TGTGAACGAAAATTTCACTT+GGG | 0.566723 | 3.4:+90747031 | MS.gene37482:CDS |
| AGATCGCCTGAGAGAATCTG+AGG | 0.577144 | 3.4:+90746250 | MS.gene37482:CDS |
| TCAGAAGTTTCTAATTCAGT+GGG | 0.578275 | 3.4:+90746401 | MS.gene37482:CDS |
| CCTGAGAGAATCTGAGGGAA+CGG | 0.579608 | 3.4:+90746256 | MS.gene37482:CDS |
| GGTATCAAGATCAACTTCAA+AGG | 0.591691 | 3.4:+90746349 | MS.gene37482:CDS |
| ATGTGCCGTTTGTTGATGAA+GGG | 0.591926 | 3.4:+90747547 | MS.gene37482:CDS |
| TTTAACAGAACCGGAACTAG+AGG | 0.594122 | 3.4:-90746309 | None:intergenic |
| CGACAGAGAGAATCCGAGGA+AGG | 0.596626 | 3.4:+90746179 | MS.gene37482:CDS |
| TTATGTCTAGAGAAACTACT+AGG | 0.598338 | 3.4:+90747163 | MS.gene37482:CDS |
| AAAGCGACAGAGAGAATCCG+AGG | 0.599141 | 3.4:+90746175 | MS.gene37482:CDS |
| GGGAAAGTACTAAAGAGGGA+AGG | 0.603607 | 3.4:+90746437 | MS.gene37482:CDS |
| TACTGCGATTATGGAGAAAG+GGG | 0.609394 | 3.4:+90747429 | MS.gene37482:CDS |
| CATATTCTACAACAGCAACT+CGG | 0.614325 | 3.4:-90746941 | None:intergenic |
| TGTGCCGTTTGTTGATGAAG+GGG | 0.619488 | 3.4:+90747548 | MS.gene37482:CDS |
| TTGACAATGTTACCAAAAGA+GGG | 0.620789 | 3.4:+90746534 | MS.gene37482:CDS |
| AGTGTCTATAGCATCCTTGG+AGG | 0.622116 | 3.4:-90747620 | None:intergenic |
| TTTGTTGATGAAGGGGCTTG+AGG | 0.622358 | 3.4:+90747555 | MS.gene37482:CDS |
| GTGAACGAAAATTTCACTTG+GGG | 0.624887 | 3.4:+90747032 | MS.gene37482:CDS |
| AAATGAAGTTTCTAGTCACA+TGG | 0.625461 | 3.4:+90747084 | MS.gene37482:CDS |
| AGAGGGAGTATCTCTTGTGA+AGG | 0.627844 | 3.4:+90746551 | MS.gene37482:CDS |
| TGAGCTCAGCCAATCAAAAG+GGG | 0.629047 | 3.4:+90746717 | MS.gene37482:CDS |
| CTGATGAGGAGTTACATCAA+AGG | 0.630815 | 3.4:+90747263 | MS.gene37482:CDS |
| GGTGACTTAATATTATGTGA+TGG | 0.647953 | 3.4:+90746842 | MS.gene37482:CDS |
| GATCGCCTGAGAGAATCTGA+GGG | 0.652065 | 3.4:+90746251 | MS.gene37482:CDS |
| GGTCCCAAACGAGATCCGGA+TGG | 0.671675 | 3.4:+90746896 | MS.gene37482:CDS |
| TGGTAACATTGTCAATACCA+CGG | 0.676702 | 3.4:-90746526 | None:intergenic |
| ACTGCGATTATGGAGAAAGG+GGG | 0.694924 | 3.4:+90747430 | MS.gene37482:CDS |
| AAGGAAGCGTAAATTGACTG+TGG | 0.704717 | 3.4:+90747282 | MS.gene37482:CDS |
| TGGGACCTAAACAATCAGAG+TGG | 0.741486 | 3.4:-90746880 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATTATTGAAAAAAATAAT+TGG | + | chr3.4:90747110-90747129 | MS.gene37482:CDS | 10.0% |
| !!! | ATCAAATCTTTTTTAGATAA+AGG | - | chr3.4:90747349-90747368 | None:intergenic | 15.0% |
| !! | AAAAAAAAATATCGAAGACA+AGG | + | chr3.4:90747523-90747542 | MS.gene37482:CDS | 20.0% |
| !! | TTATACTTTCTTTGAATTTC+AGG | + | chr3.4:90747702-90747721 | MS.gene37482:CDS | 20.0% |
| !!! | TTGTCTTCGATATTTTTTTT+TGG | - | chr3.4:90747524-90747543 | None:intergenic | 20.0% |
| ! | TAAAAAAGCTACACTAACAA+CGG | + | chr3.4:90746328-90746347 | MS.gene37482:CDS | 25.0% |
| !!! | TCTTCGATATTTTTTTTTGG+TGG | - | chr3.4:90747521-90747540 | None:intergenic | 25.0% |
| AGTTGATTAACTTAGGAGTT+GGG | + | chr3.4:90747581-90747600 | MS.gene37482:CDS | 30.0% | |
| ATCAGAAGTTTCTAATTCAG+TGG | + | chr3.4:90746400-90746419 | MS.gene37482:CDS | 30.0% | |
| ATGTGAACGAAAATTTCACT+TGG | + | chr3.4:90747030-90747049 | MS.gene37482:CDS | 30.0% | |
| ATGTGACTAGAAACTTCATT+TGG | - | chr3.4:90747085-90747104 | None:intergenic | 30.0% | |
| ATTGACAATGTTACCAAAAG+AGG | + | chr3.4:90746533-90746552 | MS.gene37482:CDS | 30.0% | |
| CATAAGGATTTATGGTCAAA+AGG | + | chr3.4:90747474-90747493 | MS.gene37482:CDS | 30.0% | |
| GGTGACTTAATATTATGTGA+TGG | + | chr3.4:90746842-90746861 | MS.gene37482:CDS | 30.0% | |
| TAGGAATACCAAATAAGCTT+GGG | + | chr3.4:90747182-90747201 | MS.gene37482:CDS | 30.0% | |
| TATAATGTGAGTTGTGATGA+TGG | + | chr3.4:90747232-90747251 | MS.gene37482:CDS | 30.0% | |
| TCAGAAGTTTCTAATTCAGT+GGG | + | chr3.4:90746401-90746420 | MS.gene37482:CDS | 30.0% | |
| TGTGAACGAAAATTTCACTT+GGG | + | chr3.4:90747031-90747050 | MS.gene37482:CDS | 30.0% | |
| TTATGTCTAGAGAAACTACT+AGG | + | chr3.4:90747163-90747182 | MS.gene37482:CDS | 30.0% | |
| TTGACAATGTTACCAAAAGA+GGG | + | chr3.4:90746534-90746553 | MS.gene37482:CDS | 30.0% | |
| TTGTAATGCTGAAGCAAAAT+CGG | + | chr3.4:90746745-90746764 | MS.gene37482:CDS | 30.0% | |
| ! | CTTTTGACCATAAATCCTTA+TGG | - | chr3.4:90747476-90747495 | None:intergenic | 30.0% |
| !! | AAAAGTATCTTCTTGGAAGA+CGG | + | chr3.4:90746656-90746675 | MS.gene37482:CDS | 30.0% |
| !! | AAATGAAGTTTCTAGTCACA+TGG | + | chr3.4:90747084-90747103 | MS.gene37482:CDS | 30.0% |
| !!! | ACATGTTCTGTTTGTTGTTT+TGG | + | chr3.4:90746818-90746837 | MS.gene37482:CDS | 30.0% |
| !!! | ATGTTCTGTTTGTTGTTTTG+GGG | + | chr3.4:90746820-90746839 | MS.gene37482:CDS | 30.0% |
| !!! | CATGTTCTGTTTGTTGTTTT+GGG | + | chr3.4:90746819-90746838 | MS.gene37482:CDS | 30.0% |
| !!! | TGTAGCTTTTTTAACAGAAC+CGG | - | chr3.4:90746321-90746340 | None:intergenic | 30.0% |
| AAGAAACGTTTGAACTTTCG+TGG | + | chr3.4:90747400-90747419 | MS.gene37482:CDS | 35.0% | |
| AGATCATTCCCAAGCTTATT+TGG | - | chr3.4:90747193-90747212 | None:intergenic | 35.0% | |
| ATACTGCGATTATGGAGAAA+GGG | + | chr3.4:90747428-90747447 | MS.gene37482:CDS | 35.0% | |
| ATTCACTCTCACCAAATTTG+AGG | + | chr3.4:90746601-90746620 | MS.gene37482:CDS | 35.0% | |
| CAGTTGATTAACTTAGGAGT+TGG | + | chr3.4:90747580-90747599 | MS.gene37482:CDS | 35.0% | |
| CATATTCTACAACAGCAACT+CGG | - | chr3.4:90746944-90746963 | None:intergenic | 35.0% | |
| CTAGGAATACCAAATAAGCT+TGG | + | chr3.4:90747181-90747200 | MS.gene37482:CDS | 35.0% | |
| GAGACACTTTCTTTGTTAAG+TGG | - | chr3.4:90747748-90747767 | None:intergenic | 35.0% | |
| GAGGAACAGTTGATTAACTT+AGG | + | chr3.4:90747574-90747593 | MS.gene37482:CDS | 35.0% | |
| GGATAAATCAACTTCTGGTA+AGG | + | chr3.4:90746277-90746296 | MS.gene37482:CDS | 35.0% | |
| GGTATCAAGATCAACTTCAA+AGG | + | chr3.4:90746349-90746368 | MS.gene37482:CDS | 35.0% | |
| GTGAACGAAAATTTCACTTG+GGG | + | chr3.4:90747032-90747051 | MS.gene37482:CDS | 35.0% | |
| TATACTGCGATTATGGAGAA+AGG | + | chr3.4:90747427-90747446 | MS.gene37482:CDS | 35.0% | |
| TGGTAACATTGTCAATACCA+CGG | - | chr3.4:90746529-90746548 | None:intergenic | 35.0% | |
| TGGTTTCTATACTGCGATTA+TGG | + | chr3.4:90747420-90747439 | MS.gene37482:CDS | 35.0% | |
| TGTGTAAAGGCAACAAGAAT+TGG | + | chr3.4:90747055-90747074 | MS.gene37482:CDS | 35.0% | |
| ! | ACAATCATTCCCCTTTTGAT+TGG | - | chr3.4:90746729-90746748 | None:intergenic | 35.0% |
| ! | GCAAATTCTTGTTTGCGTTT+GGG | - | chr3.4:90746974-90746993 | None:intergenic | 35.0% |
| ! | GTCTTCCAAGAAGATACTTT+TGG | - | chr3.4:90746657-90746676 | None:intergenic | 35.0% |
| ! | GTTCAAACGTTTCTTTTCTG+AGG | - | chr3.4:90747395-90747414 | None:intergenic | 35.0% |
| ! | TAAAGAGGGAAGGTATAAGA+GGG | + | chr3.4:90746447-90746466 | MS.gene37482:CDS | 35.0% |
| ! | TCCAAGAAGATACTTTTGGA+TGG | - | chr3.4:90746653-90746672 | None:intergenic | 35.0% |
| ! | TGCAAATTCTTGTTTGCGTT+TGG | - | chr3.4:90746975-90746994 | None:intergenic | 35.0% |
| !! | AAAGAGGGAAAGTACTAAAG+AGG | + | chr3.4:90746432-90746451 | MS.gene37482:CDS | 35.0% |
| !! | AAGAGGGAAAGTACTAAAGA+GGG | + | chr3.4:90746433-90746452 | MS.gene37482:CDS | 35.0% |
| !! | ACCATCCAAAAGTATCTTCT+TGG | + | chr3.4:90746649-90746668 | MS.gene37482:CDS | 35.0% |
| !! | GAAGTGTTCTCAGTTGATAA+AGG | + | chr3.4:90746469-90746488 | MS.gene37482:CDS | 35.0% |
| !! | GTTGATAAAGGATGGAGTTT+TGG | + | chr3.4:90746481-90746500 | MS.gene37482:CDS | 35.0% |
| !! | TGTTCTCAGTTGATAAAGGA+TGG | + | chr3.4:90746473-90746492 | MS.gene37482:CDS | 35.0% |
| !!! | TGTTCTGTTTGTTGTTTTGG+GGG | + | chr3.4:90746821-90746840 | MS.gene37482:CDS | 35.0% |
| ACAAGCATGAGCCTCAAATT+TGG | - | chr3.4:90746615-90746634 | None:intergenic | 40.0% | |
| ACATGTTCCCACAAACTTCA+TGG | - | chr3.4:90747141-90747160 | None:intergenic | 40.0% | |
| AGCTTGGGAATGATCTAACT+TGG | + | chr3.4:90747197-90747216 | MS.gene37482:CDS | 40.0% | |
| ATTTCACTTGGGGTGTGTAA+AGG | + | chr3.4:90747042-90747061 | MS.gene37482:CDS | 40.0% | |
| CTGATGAGGAGTTACATCAA+AGG | + | chr3.4:90747263-90747282 | MS.gene37482:CDS | 40.0% | |
| GGAACGGATAAATCAACTTC+TGG | + | chr3.4:90746272-90746291 | MS.gene37482:CDS | 40.0% | |
| GTGGCAACCATAAGGATTTA+TGG | + | chr3.4:90747466-90747485 | MS.gene37482:CDS | 40.0% | |
| GTTTCTAATTCAGTGGGTAG+TGG | + | chr3.4:90746407-90746426 | MS.gene37482:CDS | 40.0% | |
| TACTGCGATTATGGAGAAAG+GGG | + | chr3.4:90747429-90747448 | MS.gene37482:CDS | 40.0% | |
| TCGTTCCACTCTGATTGTTT+AGG | + | chr3.4:90746875-90746894 | MS.gene37482:CDS | 40.0% | |
| TTTAACAGAACCGGAACTAG+AGG | - | chr3.4:90746312-90746331 | None:intergenic | 40.0% | |
| ! | AAGGAAGCGTAAATTGACTG+TGG | + | chr3.4:90747282-90747301 | MS.gene37482:CDS | 40.0% |
| ! | ACAAGAGATACTCCCTCTTT+TGG | - | chr3.4:90746549-90746568 | None:intergenic | 40.0% |
| ! | CTAAAGAGGGAAGGTATAAG+AGG | + | chr3.4:90746446-90746465 | MS.gene37482:CDS | 40.0% |
| ! | GGTTGCTGAGATTGTTTTCA+TGG | + | chr3.4:90747495-90747514 | MS.gene37482:CDS | 40.0% |
| ! | GGTTTTGCCATGAAGTTTGT+GGG | + | chr3.4:90747131-90747150 | MS.gene37482:CDS | 40.0% |
| ! | TGATGGAGATTTTGCTGATG+AGG | + | chr3.4:90747249-90747268 | MS.gene37482:CDS | 40.0% |
| ! | TGTTGTTGTTGTTCCTTCCT+CGG | - | chr3.4:90746195-90746214 | None:intergenic | 40.0% |
| !! | AATGTGCCGTTTGTTGATGA+AGG | + | chr3.4:90747546-90747565 | MS.gene37482:CDS | 40.0% |
| !! | AGGCGATCTTTTGACTGTAA+TGG | - | chr3.4:90746239-90746258 | None:intergenic | 40.0% |
| !! | ATGTGCCGTTTGTTGATGAA+GGG | + | chr3.4:90747547-90747566 | MS.gene37482:CDS | 40.0% |
| !! | GTTTCTAAAGCCTCTAGTTC+CGG | + | chr3.4:90746299-90746318 | MS.gene37482:CDS | 40.0% |
| !! | TGGTTTTGCCATGAAGTTTG+TGG | + | chr3.4:90747130-90747149 | MS.gene37482:CDS | 40.0% |
| !!! | AAGGATGGAGTTTTGGTTTC+TGG | + | chr3.4:90746488-90746507 | MS.gene37482:CDS | 40.0% |
| !!! | AGGATGGAGTTTTGGTTTCT+GGG | + | chr3.4:90746489-90746508 | MS.gene37482:CDS | 40.0% |
| ACTGCGATTATGGAGAAAGG+GGG | + | chr3.4:90747430-90747449 | MS.gene37482:CDS | 45.0% | |
| AGCTCAGAGCTTTCATTCGA+CGG | - | chr3.4:90746703-90746722 | None:intergenic | 45.0% | |
| AGTGTCTATAGCATCCTTGG+AGG | - | chr3.4:90747623-90747642 | None:intergenic | 45.0% | |
| CAAGCCCCTTCATCAACAAA+CGG | - | chr3.4:90747555-90747574 | None:intergenic | 45.0% | |
| CAATCAGAGTGGAACGAAGA+AGG | - | chr3.4:90746872-90746891 | None:intergenic | 45.0% | |
| CCAAGGATGCTATAGACACT+TGG | + | chr3.4:90747623-90747642 | MS.gene37482:CDS | 45.0% | |
| CCAAGTGTCTATAGCATCCT+TGG | - | chr3.4:90747626-90747645 | None:intergenic | 45.0% | |
| CGGAACTTCATTGTTGGACT+TGG | + | chr3.4:90746676-90746695 | MS.gene37482:CDS | 45.0% | |
| CGTAAATTGACTGTGGCTCT+TGG | + | chr3.4:90747289-90747308 | MS.gene37482:CDS | 45.0% | |
| CTGAGCTCAGCCAATCAAAA+GGG | + | chr3.4:90746716-90746735 | MS.gene37482:CDS | 45.0% | |
| GGAAGACGGAACTTCATTGT+TGG | + | chr3.4:90746670-90746689 | MS.gene37482:CDS | 45.0% | |
| TAACTTAGGAGTTGGGAGCT+TGG | + | chr3.4:90747588-90747607 | MS.gene37482:CDS | 45.0% | |
| TCTGAGCTCAGCCAATCAAA+AGG | + | chr3.4:90746715-90746734 | MS.gene37482:CDS | 45.0% | |
| TGAGCTCAGCCAATCAAAAG+GGG | + | chr3.4:90746717-90746736 | MS.gene37482:CDS | 45.0% | |
| TGAGTTCTGTGGCAACCATA+AGG | + | chr3.4:90747458-90747477 | MS.gene37482:CDS | 45.0% | |
| TGGGACCTAAACAATCAGAG+TGG | - | chr3.4:90746883-90746902 | None:intergenic | 45.0% | |
| TTTAGGTCCCAAACGAGATC+CGG | + | chr3.4:90746892-90746911 | MS.gene37482:CDS | 45.0% | |
| ! | CAGTGGGTAGTGGTAAAAGA+GGG | + | chr3.4:90746417-90746436 | MS.gene37482:CDS | 45.0% |
| ! | TCAGTGGGTAGTGGTAAAAG+AGG | + | chr3.4:90746416-90746435 | MS.gene37482:CDS | 45.0% |
| ! | TTTGTTGATGAAGGGGCTTG+AGG | + | chr3.4:90747555-90747574 | MS.gene37482:CDS | 45.0% |
| !! | AGAGGGAGTATCTCTTGTGA+AGG | + | chr3.4:90746551-90746570 | MS.gene37482:CDS | 45.0% |
| !! | GGGAAAGTACTAAAGAGGGA+AGG | + | chr3.4:90746437-90746456 | MS.gene37482:CDS | 45.0% |
| !! | TGTGCCGTTTGTTGATGAAG+GGG | + | chr3.4:90747548-90747567 | MS.gene37482:CDS | 45.0% |
| !!! | CTTGGTTTTGCATTCCTCCA+AGG | + | chr3.4:90747606-90747625 | MS.gene37482:CDS | 45.0% |
| AAAGCGACAGAGAGAATCCG+AGG | + | chr3.4:90746175-90746194 | MS.gene37482:CDS | 50.0% | |
| AGACCATCCGGATCTCGTTT+GGG | - | chr3.4:90746902-90746921 | None:intergenic | 50.0% | |
| AGATCGCCTGAGAGAATCTG+AGG | + | chr3.4:90746250-90746269 | MS.gene37482:CDS | 50.0% | |
| AGGGGGTAAAGTGAGTTCTG+TGG | + | chr3.4:90747447-90747466 | MS.gene37482:CDS | 50.0% | |
| CATGTACCATCCAGACCATC+CGG | - | chr3.4:90746914-90746933 | None:intergenic | 50.0% | |
| CCTGAGAGAATCTGAGGGAA+CGG | + | chr3.4:90746256-90746275 | MS.gene37482:CDS | 50.0% | |
| GATCGCCTGAGAGAATCTGA+GGG | + | chr3.4:90746251-90746270 | MS.gene37482:CDS | 50.0% | |
| ! | GGGACTATTGTTTTCTGCCG+TGG | + | chr3.4:90746509-90746528 | MS.gene37482:CDS | 50.0% |
| CAAACGAGATCCGGATGGTC+TGG | + | chr3.4:90746901-90746920 | MS.gene37482:CDS | 55.0% | |
| CAGACCATCCGGATCTCGTT+TGG | - | chr3.4:90746903-90746922 | None:intergenic | 55.0% | |
| CCGTTCCCTCAGATTCTCTC+AGG | - | chr3.4:90746259-90746278 | None:intergenic | 55.0% | |
| CGACAGAGAGAATCCGAGGA+AGG | + | chr3.4:90746179-90746198 | MS.gene37482:CDS | 55.0% | |
| CGGATGGTCTGGATGGTACA+TGG | + | chr3.4:90746912-90746931 | MS.gene37482:CDS | 55.0% | |
| ! | GGTGTGCTGCGTGAGAGTTT+TGG | + | chr3.4:90747310-90747329 | MS.gene37482:CDS | 55.0% |
| CGAGATCCGGATGGTCTGGA+TGG | + | chr3.4:90746905-90746924 | MS.gene37482:CDS | 60.0% | |
| GGTCCCAAACGAGATCCGGA+TGG | + | chr3.4:90746896-90746915 | MS.gene37482:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 90746146 | 90747777 | 90746146 | ID=MS.gene37482 |
| chr3.4 | mRNA | 90746146 | 90747777 | 90746146 | ID=MS.gene37482.t1;Parent=MS.gene37482 |
| chr3.4 | exon | 90746146 | 90747777 | 90746146 | ID=MS.gene37482.t1.exon1;Parent=MS.gene37482.t1 |
| chr3.4 | CDS | 90746146 | 90747777 | 90746146 | ID=cds.MS.gene37482.t1;Parent=MS.gene37482.t1 |
| Gene Sequence |
| Protein sequence |
