Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37597.t1 | XP_003618052.1 | 74.2 | 248 | 60 | 2 | 1 | 248 | 27 | 270 | 2.30E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37597.t1 | G7K0M6 | 74.2 | 248 | 60 | 2 | 1 | 248 | 27 | 270 | 1.7e-78 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37597.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37597.t1 | MTR_5g098470 | 74.597 | 248 | 59 | 2 | 1 | 248 | 27 | 270 | 3.92e-128 | 365 |
| MS.gene37597.t1 | MTR_1g046810 | 28.763 | 299 | 137 | 14 | 36 | 288 | 37 | 305 | 9.42e-14 | 70.5 |
| MS.gene37597.t1 | MTR_6g081160 | 28.481 | 158 | 95 | 4 | 40 | 187 | 30 | 179 | 3.81e-12 | 65.5 |
| MS.gene37597.t1 | MTR_1g052185 | 30.476 | 210 | 112 | 6 | 19 | 218 | 9 | 194 | 5.20e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37597.t1 | AT5G56200 | 46.429 | 84 | 41 | 1 | 38 | 117 | 77 | 160 | 6.18e-14 | 72.0 |
Find 45 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGACGATGATTCAAAATTT+TGG | 0.153043 | 5.3:+71350497 | MS.gene37597:CDS |
| TCAACATGATGATGACTATT+TGG | 0.187915 | 5.3:-71350130 | None:intergenic |
| ATACCCCTCCATTCCCTTTC+AGG | 0.219356 | 5.3:-71350385 | None:intergenic |
| GTGACCATATAAGGCCTTAT+TGG | 0.260984 | 5.3:-71350350 | None:intergenic |
| CTTCTTCTCTATGGATTTCA+TGG | 0.270549 | 5.3:-71350056 | None:intergenic |
| TTTAGGTGTGGAAAAGCATT+AGG | 0.279524 | 5.3:+71350202 | MS.gene37597:CDS |
| CCATCCAATAAGGCCTTATA+TGG | 0.323281 | 5.3:+71350346 | MS.gene37597:CDS |
| AGATCTCATGTGACCATATA+AGG | 0.346698 | 5.3:-71350359 | None:intergenic |
| TGCAATTCTTCTAATGATAG+TGG | 0.351010 | 5.3:+71350289 | MS.gene37597:CDS |
| CACTCGTTCAAATCAAAATC+TGG | 0.351225 | 5.3:-71350901 | None:intergenic |
| GATCCCATCTTCAAGCTCTA+AGG | 0.358194 | 5.3:+71350236 | MS.gene37597:CDS |
| AAAGAATTTCCCATCCAATA+AGG | 0.359792 | 5.3:+71350336 | MS.gene37597:CDS |
| GAGGGGTATGTATCCTAATA+AGG | 0.367738 | 5.3:+71350399 | MS.gene37597:CDS |
| TTAGGTGTGGAAAAGCATTA+GGG | 0.371642 | 5.3:+71350203 | MS.gene37597:CDS |
| CCTAATAAGGATAAAACTAT+TGG | 0.385116 | 5.3:+71350412 | MS.gene37597:CDS |
| CATCATGTTGAAGGATCAAC+AGG | 0.420630 | 5.3:+71350142 | MS.gene37597:CDS |
| CTTTCCTTAGAGCTTGAAGA+TGG | 0.421663 | 5.3:-71350240 | None:intergenic |
| CCTGGTACAAGTGGTCTTGT+TGG | 0.433511 | 5.3:+71350673 | MS.gene37597:CDS |
| GCAAAGAGATGAATTGTAAT+GGG | 0.433880 | 5.3:+71350833 | MS.gene37597:CDS |
| AATAGTCATCATCATGTTGA+AGG | 0.464152 | 5.3:+71350133 | MS.gene37597:CDS |
| TACCCCTCCATTCCCTTTCA+GGG | 0.465209 | 5.3:-71350384 | None:intergenic |
| CCACCAATATGGTTTAAAAC+CGG | 0.477481 | 5.3:+71350520 | MS.gene37597:CDS |
| CCATATAAGGCCTTATTGGA+TGG | 0.492046 | 5.3:-71350346 | None:intergenic |
| CATGAGATCTCACCCTGAAA+GGG | 0.502988 | 5.3:+71350372 | MS.gene37597:CDS |
| TGCCATCATAAGTTCCACTT+TGG | 0.505420 | 5.3:-71350813 | None:intergenic |
| GGCAAAGAGATGAATTGTAA+TGG | 0.511018 | 5.3:+71350832 | MS.gene37597:CDS |
| TTCGGATGACGAGGATAAGA+AGG | 0.520435 | 5.3:+71350639 | MS.gene37597:CDS |
| ACATGAGATCTCACCCTGAA+AGG | 0.521597 | 5.3:+71350371 | MS.gene37597:CDS |
| TGGTTTAAAACCGGCAAAAG+AGG | 0.523461 | 5.3:+71350529 | MS.gene37597:CDS |
| TGACAACTTCAATGCAGATT+CGG | 0.527965 | 5.3:+71350621 | MS.gene37597:CDS |
| AGAATGACAATAACAAAAGA+AGG | 0.532053 | 5.3:+71350719 | MS.gene37597:CDS |
| TACCAAAGTGGAACTTATGA+TGG | 0.540351 | 5.3:+71350811 | MS.gene37597:CDS |
| CCAACAAGACCACTTGTACC+AGG | 0.547377 | 5.3:-71350673 | None:intergenic |
| TTTCCTTAGAGCTTGAAGAT+GGG | 0.556262 | 5.3:-71350239 | None:intergenic |
| TCTTCAAGCTCTAAGGAAAG+AGG | 0.559146 | 5.3:+71350243 | MS.gene37597:CDS |
| CATATAAGGCCTTATTGGAT+GGG | 0.559243 | 5.3:-71350345 | None:intergenic |
| GATCTCACCCTGAAAGGGAA+TGG | 0.563550 | 5.3:+71350377 | MS.gene37597:CDS |
| TCACCCTGAAAGGGAATGGA+GGG | 0.580204 | 5.3:+71350381 | MS.gene37597:CDS |
| CTCACCCTGAAAGGGAATGG+AGG | 0.583418 | 5.3:+71350380 | MS.gene37597:CDS |
| CTTCAAGCTCTAAGGAAAGA+GGG | 0.588989 | 5.3:+71350244 | MS.gene37597:CDS |
| CAATGCAGATTCGGATGACG+AGG | 0.631053 | 5.3:+71350630 | MS.gene37597:CDS |
| AGGTGTGGAAAAGCATTAGG+GGG | 0.631959 | 5.3:+71350205 | MS.gene37597:CDS |
| TAGGTGTGGAAAAGCATTAG+GGG | 0.641738 | 5.3:+71350204 | MS.gene37597:CDS |
| CACCCTGAAAGGGAATGGAG+GGG | 0.655548 | 5.3:+71350382 | MS.gene37597:CDS |
| AGAGGGTGAGTTGAAAACAA+AGG | 0.686503 | 5.3:+71350261 | MS.gene37597:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGTAACAAAATTTTT+AGG | + | chr5.3:71350185-71350204 | MS.gene37597:CDS | 10.0% |
| !! | ATCTAAAAACAAAATCTTGT+TGG | + | chr5.3:71350777-71350796 | MS.gene37597:CDS | 20.0% |
| !!! | TTGTTTTTAGATATTGAACA+TGG | - | chr5.3:71350769-71350788 | None:intergenic | 20.0% |
| ! | AGAATGACAATAACAAAAGA+AGG | + | chr5.3:71350719-71350738 | MS.gene37597:CDS | 25.0% |
| ! | CCTAATAAGGATAAAACTAT+TGG | + | chr5.3:71350412-71350431 | MS.gene37597:CDS | 25.0% |
| !! | CAAATCTTTTGAATTGATTG+CGG | - | chr5.3:71350473-71350492 | None:intergenic | 25.0% |
| !! | TAAACTTTTCTTCTTCTCTA+TGG | - | chr5.3:71350068-71350087 | None:intergenic | 25.0% |
| !!! | CCAATAGTTTTATCCTTATT+AGG | - | chr5.3:71350415-71350434 | None:intergenic | 25.0% |
| !!! | TGTAACAAAATTTTTAGGTG+TGG | + | chr5.3:71350190-71350209 | MS.gene37597:CDS | 25.0% |
| AAAGAATTTCCCATCCAATA+AGG | + | chr5.3:71350336-71350355 | MS.gene37597:CDS | 30.0% | |
| AATAGTCATCATCATGTTGA+AGG | + | chr5.3:71350133-71350152 | MS.gene37597:CDS | 30.0% | |
| GCAAAGAGATGAATTGTAAT+GGG | + | chr5.3:71350833-71350852 | MS.gene37597:CDS | 30.0% | |
| TAACAAAAGAAGGAAAAAGC+TGG | + | chr5.3:71350729-71350748 | MS.gene37597:CDS | 30.0% | |
| TGCAATTCTTCTAATGATAG+TGG | + | chr5.3:71350289-71350308 | MS.gene37597:CDS | 30.0% | |
| ! | AAGAAGGAGAATATTTTACC+TGG | + | chr5.3:71350655-71350674 | MS.gene37597:CDS | 30.0% |
| ! | AATATTTTACCTGGTACAAG+TGG | + | chr5.3:71350664-71350683 | MS.gene37597:CDS | 30.0% |
| ! | TCAACATGATGATGACTATT+TGG | - | chr5.3:71350133-71350152 | None:intergenic | 30.0% |
| ! | TCGACGATGATTCAAAATTT+TGG | + | chr5.3:71350497-71350516 | MS.gene37597:CDS | 30.0% |
| !!! | GTAATGGGTATTTTGAGATT+GGG | + | chr5.3:71350848-71350867 | MS.gene37597:CDS | 30.0% |
| !!! | TAATGGGTATTTTGAGATTG+GGG | + | chr5.3:71350849-71350868 | MS.gene37597:CDS | 30.0% |
| !!! | TGTAATGGGTATTTTGAGAT+TGG | + | chr5.3:71350847-71350866 | MS.gene37597:CDS | 30.0% |
| AGATCTCATGTGACCATATA+AGG | - | chr5.3:71350362-71350381 | None:intergenic | 35.0% | |
| CACTCGTTCAAATCAAAATC+TGG | - | chr5.3:71350904-71350923 | None:intergenic | 35.0% | |
| GAAAAAGATTGCTACCAAAG+TGG | + | chr5.3:71350799-71350818 | MS.gene37597:CDS | 35.0% | |
| GGCAAAGAGATGAATTGTAA+TGG | + | chr5.3:71350832-71350851 | MS.gene37597:CDS | 35.0% | |
| TACCAAAGTGGAACTTATGA+TGG | + | chr5.3:71350811-71350830 | MS.gene37597:CDS | 35.0% | |
| TGACAACTTCAATGCAGATT+CGG | + | chr5.3:71350621-71350640 | MS.gene37597:CDS | 35.0% | |
| TTTCCTTAGAGCTTGAAGAT+GGG | - | chr5.3:71350242-71350261 | None:intergenic | 35.0% | |
| ! | CCACCAATATGGTTTAAAAC+CGG | + | chr5.3:71350520-71350539 | MS.gene37597:CDS | 35.0% |
| !! | CAAAATTTTGGCCACCAATA+TGG | + | chr5.3:71350509-71350528 | MS.gene37597:CDS | 35.0% |
| !! | CATATAAGGCCTTATTGGAT+GGG | - | chr5.3:71350348-71350367 | None:intergenic | 35.0% |
| !! | TTAGGTGTGGAAAAGCATTA+GGG | + | chr5.3:71350203-71350222 | MS.gene37597:CDS | 35.0% |
| !! | TTTAGGTGTGGAAAAGCATT+AGG | + | chr5.3:71350202-71350221 | MS.gene37597:CDS | 35.0% |
| !!! | TTGCCGGTTTTAAACCATAT+TGG | - | chr5.3:71350526-71350545 | None:intergenic | 35.0% |
| CATCATGTTGAAGGATCAAC+AGG | + | chr5.3:71350142-71350161 | MS.gene37597:CDS | 40.0% | |
| CTTTCCTTAGAGCTTGAAGA+TGG | - | chr5.3:71350243-71350262 | None:intergenic | 40.0% | |
| TGCCATCATAAGTTCCACTT+TGG | - | chr5.3:71350816-71350835 | None:intergenic | 40.0% | |
| ! | AGAGGGTGAGTTGAAAACAA+AGG | + | chr5.3:71350261-71350280 | MS.gene37597:CDS | 40.0% |
| ! | CCATCCAATAAGGCCTTATA+TGG | + | chr5.3:71350346-71350365 | MS.gene37597:CDS | 40.0% |
| ! | GAGGGGTATGTATCCTAATA+AGG | + | chr5.3:71350399-71350418 | MS.gene37597:CDS | 40.0% |
| ! | GTGACCATATAAGGCCTTAT+TGG | - | chr5.3:71350353-71350372 | None:intergenic | 40.0% |
| ! | TGGTTTAAAACCGGCAAAAG+AGG | + | chr5.3:71350529-71350548 | MS.gene37597:CDS | 40.0% |
| !! | CCATATAAGGCCTTATTGGA+TGG | - | chr5.3:71350349-71350368 | None:intergenic | 40.0% |
| !! | CTTCAAGCTCTAAGGAAAGA+GGG | + | chr5.3:71350244-71350263 | MS.gene37597:CDS | 40.0% |
| !! | TAGGTGTGGAAAAGCATTAG+GGG | + | chr5.3:71350204-71350223 | MS.gene37597:CDS | 40.0% |
| !! | TCTTCAAGCTCTAAGGAAAG+AGG | + | chr5.3:71350243-71350262 | MS.gene37597:CDS | 40.0% |
| !!! | CCGGTTTTAAACCATATTGG+TGG | - | chr5.3:71350523-71350542 | None:intergenic | 40.0% |
| ACATGAGATCTCACCCTGAA+AGG | + | chr5.3:71350371-71350390 | MS.gene37597:CDS | 45.0% | |
| CATGAGATCTCACCCTGAAA+GGG | + | chr5.3:71350372-71350391 | MS.gene37597:CDS | 45.0% | |
| GATCCCATCTTCAAGCTCTA+AGG | + | chr5.3:71350236-71350255 | MS.gene37597:CDS | 45.0% | |
| TTCGGATGACGAGGATAAGA+AGG | + | chr5.3:71350639-71350658 | MS.gene37597:CDS | 45.0% | |
| !! | AGGTGTGGAAAAGCATTAGG+GGG | + | chr5.3:71350205-71350224 | MS.gene37597:CDS | 45.0% |
| ATACCCCTCCATTCCCTTTC+AGG | - | chr5.3:71350388-71350407 | None:intergenic | 50.0% | |
| CAATGCAGATTCGGATGACG+AGG | + | chr5.3:71350630-71350649 | MS.gene37597:CDS | 50.0% | |
| CCAACAAGACCACTTGTACC+AGG | - | chr5.3:71350676-71350695 | None:intergenic | 50.0% | |
| GATCTCACCCTGAAAGGGAA+TGG | + | chr5.3:71350377-71350396 | MS.gene37597:CDS | 50.0% | |
| TACCCCTCCATTCCCTTTCA+GGG | - | chr5.3:71350387-71350406 | None:intergenic | 50.0% | |
| TCACCCTGAAAGGGAATGGA+GGG | + | chr5.3:71350381-71350400 | MS.gene37597:CDS | 50.0% | |
| ! | GCTAGAACGTCCTCTTTTGC+CGG | - | chr5.3:71350542-71350561 | None:intergenic | 50.0% |
| !! | CCTGGTACAAGTGGTCTTGT+TGG | + | chr5.3:71350673-71350692 | MS.gene37597:CDS | 50.0% |
| CACCCTGAAAGGGAATGGAG+GGG | + | chr5.3:71350382-71350401 | MS.gene37597:CDS | 55.0% | |
| CTCACCCTGAAAGGGAATGG+AGG | + | chr5.3:71350380-71350399 | MS.gene37597:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 71350058 | 71350924 | 71350058 | ID=MS.gene37597 |
| chr5.3 | mRNA | 71350058 | 71350924 | 71350058 | ID=MS.gene37597.t1;Parent=MS.gene37597 |
| chr5.3 | exon | 71350058 | 71350924 | 71350058 | ID=MS.gene37597.t1.exon1;Parent=MS.gene37597.t1 |
| chr5.3 | CDS | 71350058 | 71350924 | 71350058 | ID=cds.MS.gene37597.t1;Parent=MS.gene37597.t1 |
| Gene Sequence |
| Protein sequence |
