Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37599.t1 | AES92029.1 | 52.8 | 193 | 12 | 1 | 12 | 125 | 31 | 223 | 7.70E-47 | 196.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37599.t1 | G7JQ00 | 52.8 | 193 | 12 | 1 | 12 | 125 | 31 | 223 | 5.5e-47 | 196.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37599.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37599.t1 | MTR_4g123470 | 89.024 | 82 | 9 | 0 | 44 | 125 | 142 | 223 | 5.55e-51 | 161 |
| MS.gene37599.t1 | MTR_4g123470 | 82.857 | 35 | 6 | 0 | 12 | 46 | 31 | 65 | 1.17e-14 | 67.8 |
| MS.gene37599.t1 | MTR_7g113060 | 89.189 | 37 | 4 | 0 | 89 | 125 | 3 | 39 | 5.68e-18 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGTGCATGTAGAAAATC+AGG | 0.323162 | 5.3:+71317525 | MS.gene37599:CDS |
| AGGAATTTGCCGAAAGTATC+AGG | 0.335653 | 5.3:-71318171 | None:intergenic |
| AATCGTGATGTTTCACTCTA+AGG | 0.341127 | 5.3:-71318191 | None:intergenic |
| TAGGAACTACCTGATACTTT+CGG | 0.400617 | 5.3:+71318162 | MS.gene37599:intron |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 5.3:-71318277 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 5.3:-71318276 | None:intergenic |
| GAGCAATGGCACTTGCTAGA+TGG | 0.455163 | 5.3:+71317770 | MS.gene37599:CDS |
| TTGTGCTGCCAATGGTATTG+AGG | 0.463000 | 5.3:+71318317 | MS.gene37599:CDS |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 5.3:+71318282 | MS.gene37599:CDS |
| TAAATATTTGTACGAGCTAC+CGG | 0.476243 | 5.3:+71317748 | MS.gene37599:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 5.3:+71318276 | MS.gene37599:CDS |
| TGTGCTGCCAATGGTATTGA+GGG | 0.501205 | 5.3:+71318318 | MS.gene37599:CDS |
| ACGGAAATGATATTGTCATA+AGG | 0.522697 | 5.3:-71318231 | None:intergenic |
| TCTAGCAAGTGCCATTGCTC+CGG | 0.534587 | 5.3:-71317767 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 5.3:-71318275 | None:intergenic |
| TTTCAAGTGCAAGTGCGTCA+TGG | 0.567746 | 5.3:+71318356 | MS.gene37599:CDS |
| GTCTCCGCCCTCAATACCAT+TGG | 0.581950 | 5.3:-71318325 | None:intergenic |
| GTCTGTATATTTGAGTCTCA+CGG | 0.587084 | 5.3:-71318250 | None:intergenic |
| TGGCACTTGCTAGATGGTGA+TGG | 0.587936 | 5.3:+71317776 | MS.gene37599:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 5.3:+71318286 | MS.gene37599:CDS |
| GGCACTTGCTAGATGGTGAT+GGG | 0.608333 | 5.3:+71317777 | MS.gene37599:CDS |
| TGTACGAGCTACCGGAGCAA+TGG | 0.612121 | 5.3:+71317756 | MS.gene37599:CDS |
| GCTGCCAATGGTATTGAGGG+CGG | 0.739556 | 5.3:+71318321 | MS.gene37599:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTATATATGCTATAATAA+AGG | + | chr5.3:71318102-71318121 | MS.gene37599:intron | 15.0% |
| !!! | CAGATTTTTTTCTTTTTTTT+AGG | + | chr5.3:71318143-71318162 | MS.gene37599:intron | 15.0% |
| !! | AAAAAACAATAAACAACCTT+CGG | - | chr5.3:71318062-71318081 | None:intergenic | 20.0% |
| !! | TTAGACAGTGTATTAAAAAA+AGG | - | chr5.3:71317690-71317709 | None:intergenic | 20.0% |
| !! | TTCTATTTAAATACGATGAA+GGG | + | chr5.3:71317575-71317594 | MS.gene37599:intron | 20.0% |
| !! | TTTCTATTTAAATACGATGA+AGG | + | chr5.3:71317574-71317593 | MS.gene37599:intron | 20.0% |
| !! | ATGTGCATGAAAATAACTTT+TGG | + | chr5.3:71317614-71317633 | MS.gene37599:intron | 25.0% |
| !! | TTTTGATGCTATTCATTTAG+AGG | + | chr5.3:71318080-71318099 | MS.gene37599:intron | 25.0% |
| ACGGAAATGATATTGTCATA+AGG | - | chr5.3:71318234-71318253 | None:intergenic | 30.0% | |
| TAAATATTTGTACGAGCTAC+CGG | + | chr5.3:71317748-71317767 | MS.gene37599:CDS | 30.0% | |
| TAATACACTGTCTAACTTCT+TGG | + | chr5.3:71317695-71317714 | MS.gene37599:intron | 30.0% | |
| ! | GCATGAAAATAACTTTTGGT+TGG | + | chr5.3:71317618-71317637 | MS.gene37599:intron | 30.0% |
| AATCGTGATGTTTCACTCTA+AGG | - | chr5.3:71318194-71318213 | None:intergenic | 35.0% | |
| ATTATTGCTTTCCAGTACCT+TGG | + | chr5.3:71317899-71317918 | MS.gene37599:intron | 35.0% | |
| GTCTGTATATTTGAGTCTCA+CGG | - | chr5.3:71318253-71318272 | None:intergenic | 35.0% | |
| TAGGAACTACCTGATACTTT+CGG | + | chr5.3:71318162-71318181 | MS.gene37599:intron | 35.0% | |
| TATCCAGATAGGAACTTCAA+AGG | - | chr5.3:71318023-71318042 | None:intergenic | 35.0% | |
| TTGAAGTTCCTATCTGGATA+TGG | + | chr5.3:71318023-71318042 | MS.gene37599:intron | 35.0% | |
| ! | TTTGAAATCTACTTCCACCA+AGG | - | chr5.3:71317919-71317938 | None:intergenic | 35.0% |
| ATGTGATGTTCGGAAAAGGT+TGG | + | chr5.3:71318286-71318305 | MS.gene37599:CDS | 40.0% | |
| CACAGTGCATGTAGAAAATC+AGG | + | chr5.3:71317525-71317544 | MS.gene37599:CDS | 40.0% | |
| CCAAATGTGATGTTCGGAAA+AGG | + | chr5.3:71318282-71318301 | MS.gene37599:CDS | 40.0% | |
| CCCATACCAAATGTGATGTT+CGG | + | chr5.3:71318276-71318295 | MS.gene37599:CDS | 40.0% | |
| CCGAACATCACATTTGGTAT+GGG | - | chr5.3:71318279-71318298 | None:intergenic | 40.0% | |
| CGAACATCACATTTGGTATG+GGG | - | chr5.3:71318278-71318297 | None:intergenic | 40.0% | |
| TCCGAACATCACATTTGGTA+TGG | - | chr5.3:71318280-71318299 | None:intergenic | 40.0% | |
| ! | CCTTTTCCGAACATCACATT+TGG | - | chr5.3:71318285-71318304 | None:intergenic | 40.0% |
| !! | AGGAATTTGCCGAAAGTATC+AGG | - | chr5.3:71318174-71318193 | None:intergenic | 40.0% |
| !! | GTACCTTTGAAGTTCCTATC+TGG | + | chr5.3:71318017-71318036 | MS.gene37599:intron | 40.0% |
| ACCCTATCATTCCATGAGTG+CGG | + | chr5.3:71317979-71317998 | MS.gene37599:intron | 45.0% | |
| ATCTACTTCCACCAAGGTAC+TGG | - | chr5.3:71317913-71317932 | None:intergenic | 45.0% | |
| ATTGCTTTCCAGTACCTTGG+TGG | + | chr5.3:71317902-71317921 | MS.gene37599:intron | 45.0% | |
| CTCCAACCATCTGTCAACAA+CGG | - | chr5.3:71317842-71317861 | None:intergenic | 45.0% | |
| TCGTCGATCCATATCCAGAT+AGG | - | chr5.3:71318034-71318053 | None:intergenic | 45.0% | |
| TGATAGGGTGGATAATCAGC+TGG | - | chr5.3:71317968-71317987 | None:intergenic | 45.0% | |
| TTGTGCTGCCAATGGTATTG+AGG | + | chr5.3:71318317-71318336 | MS.gene37599:CDS | 45.0% | |
| ! | ACGAGTTTTTGTGCTGCCAA+TGG | + | chr5.3:71318309-71318328 | MS.gene37599:CDS | 45.0% |
| ! | TGTGCTGCCAATGGTATTGA+GGG | + | chr5.3:71318318-71318337 | MS.gene37599:CDS | 45.0% |
| ! | TTTCAAGTGCAAGTGCGTCA+TGG | + | chr5.3:71318356-71318375 | MS.gene37599:CDS | 45.0% |
| AGCCGTTGTTGACAGATGGT+TGG | + | chr5.3:71317837-71317856 | MS.gene37599:intron | 50.0% | |
| AGTCAGCCGTTGTTGACAGA+TGG | + | chr5.3:71317833-71317852 | MS.gene37599:intron | 50.0% | |
| GCACTCATGGAATGATAGGG+TGG | - | chr5.3:71317980-71317999 | None:intergenic | 50.0% | |
| GCCGCACTCATGGAATGATA+GGG | - | chr5.3:71317983-71318002 | None:intergenic | 50.0% | |
| TGCCGCACTCATGGAATGAT+AGG | - | chr5.3:71317984-71318003 | None:intergenic | 50.0% | |
| TGGATCGACGAAATCACCGA+AGG | + | chr5.3:71318043-71318062 | MS.gene37599:intron | 50.0% | |
| ! | GAGCAATGGCACTTGCTAGA+TGG | + | chr5.3:71317770-71317789 | MS.gene37599:CDS | 50.0% |
| ! | GGCACTTGCTAGATGGTGAT+GGG | + | chr5.3:71317777-71317796 | MS.gene37599:CDS | 50.0% |
| ! | TCTAGCAAGTGCCATTGCTC+CGG | - | chr5.3:71317770-71317789 | None:intergenic | 50.0% |
| ! | TGGCACTTGCTAGATGGTGA+TGG | + | chr5.3:71317776-71317795 | MS.gene37599:CDS | 50.0% |
| GTCTCCGCCCTCAATACCAT+TGG | - | chr5.3:71318328-71318347 | None:intergenic | 55.0% | |
| TGTACGAGCTACCGGAGCAA+TGG | + | chr5.3:71317756-71317775 | MS.gene37599:CDS | 55.0% | |
| ! | GCTGCCAATGGTATTGAGGG+CGG | + | chr5.3:71318321-71318340 | MS.gene37599:CDS | 55.0% |
| ACACGATGCTGCCGCACTCA+TGG | - | chr5.3:71317993-71318012 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 71317513 | 71318404 | 71317513 | ID=MS.gene37599 |
| chr5.3 | mRNA | 71317513 | 71318404 | 71317513 | ID=MS.gene37599.t1;Parent=MS.gene37599 |
| chr5.3 | exon | 71317513 | 71317546 | 71317513 | ID=MS.gene37599.t1.exon1;Parent=MS.gene37599.t1 |
| chr5.3 | CDS | 71317513 | 71317546 | 71317513 | ID=cds.MS.gene37599.t1;Parent=MS.gene37599.t1 |
| chr5.3 | exon | 71317726 | 71317829 | 71317726 | ID=MS.gene37599.t1.exon2;Parent=MS.gene37599.t1 |
| chr5.3 | CDS | 71317726 | 71317829 | 71317726 | ID=cds.MS.gene37599.t1;Parent=MS.gene37599.t1 |
| chr5.3 | exon | 71318165 | 71318404 | 71318165 | ID=MS.gene37599.t1.exon3;Parent=MS.gene37599.t1 |
| chr5.3 | CDS | 71318165 | 71318404 | 71318165 | ID=cds.MS.gene37599.t1;Parent=MS.gene37599.t1 |
| Gene Sequence |
| Protein sequence |
