AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37894

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37894.t1 MTR_5g086040 78.448 116 13 1 113 228 153 256 2.85e-55 181
MS.gene37894.t1 MTR_5g086040 93.333 90 6 0 55 144 1 90 1.50e-42 148
MS.gene37894.t1 MTR_5g086040 78.448 116 13 1 113 228 260 363 2.81e-54 181
MS.gene37894.t1 MTR_5g086040 93.069 101 7 0 44 144 97 197 1.58e-50 171
MS.gene37894.t1 MTR_5g086040 78.448 116 13 1 113 228 412 515 6.74e-53 181
MS.gene37894.t1 MTR_5g086040 93.069 101 7 0 44 144 249 349 3.23e-49 171
MS.gene37894.t1 MTR_5g086040 93.069 101 7 0 44 144 287 387 1.84e-49 171
MS.gene37894.t1 MTR_5g086040 76.699 103 12 1 113 215 450 540 7.61e-43 153
MS.gene37894.t1 MTR_3g080050 54.032 124 42 1 38 146 258 381 8.49e-32 123
MS.gene37894.t1 MTR_3g080050 59.574 94 31 4 140 229 457 547 4.00e-22 95.5
MS.gene37894.t1 MTR_5g086030 73.171 82 22 0 63 144 316 397 1.51e-31 122
MS.gene37894.t1 MTR_5g086030 56.757 111 31 3 113 220 460 556 9.24e-27 108
MS.gene37894.t1 MTR_5g086130 51.145 131 47 1 31 144 247 377 8.17e-30 117
MS.gene37894.t1 MTR_5g086130 60.215 93 29 5 140 228 455 543 2.62e-22 96.3
MS.gene37894.t1 MTR_5g086130 70.370 81 24 0 64 144 299 379 8.38e-30 117
MS.gene37894.t1 MTR_5g086130 60.215 93 29 5 140 228 457 545 2.60e-22 96.3
MS.gene37894.t1 MTR_2g024290 61.053 95 37 0 50 144 281 375 1.17e-28 114
MS.gene37894.t1 MTR_2g024290 57.500 80 27 4 140 215 453 529 5.40e-16 77.4
MS.gene37894.t1 MTR_2g024290 61.053 95 37 0 50 144 281 375 1.66e-28 114
MS.gene37894.t1 MTR_2g024290 56.383 94 33 5 140 229 453 542 4.20e-20 89.7
MS.gene37894.t1 MTR_5g086080 69.231 78 24 0 67 144 126 203 2.32e-28 112
MS.gene37894.t1 MTR_5g086080 51.402 107 33 3 140 229 281 385 2.52e-21 92.8
MS.gene37894.t1 MTR_5g086090 67.949 78 25 0 67 144 286 363 6.13e-28 112
MS.gene37894.t1 MTR_5g086090 51.887 106 32 3 140 228 441 544 1.73e-20 90.9
MS.gene37894.t1 MTR_5g086540 65.385 78 27 0 67 144 254 331 4.74e-27 109
MS.gene37894.t1 MTR_5g086540 51.456 103 34 3 140 228 409 509 1.93e-21 93.6
MS.gene37894.t1 MTR_5g086310 71.831 71 20 0 62 132 239 309 1.65e-25 103
MS.gene37894.t1 MTR_5g086120 51.754 114 48 1 38 144 251 364 4.15e-24 101
MS.gene37894.t1 MTR_5g086120 51.923 104 33 2 140 228 442 543 8.68e-22 94.7
MS.gene37894.t1 MTR_5g086330 60.215 93 29 5 140 228 85 173 2.39e-23 95.5
MS.gene37894.t1 MTR_8g463990 37.427 171 73 5 61 199 18 186 1.56e-21 92.0
MS.gene37894.t1 MTR_5g078590 36.913 149 43 4 9 150 46 150 4.49e-16 74.3
MS.gene37894.t1 MTR_8g470170 33.333 147 79 5 73 202 17 161 2.23e-14 71.2
MS.gene37894.t1 MTR_5g033490 44.000 75 42 0 70 144 292 366 6.91e-14 71.2
MS.gene37894.t1 MTR_8g014500 42.683 82 47 0 69 150 339 420 1.06e-13 70.9
MS.gene37894.t1 MTR_8g014500 42.683 82 47 0 69 150 339 420 1.11e-13 70.9
MS.gene37894.t1 MTR_2g064940 51.351 74 32 2 75 144 31 104 1.13e-13 70.5
MS.gene37894.t1 MTR_8g014500 42.683 82 47 0 69 150 339 420 1.37e-13 70.5
MS.gene37894.t1 MTR_2g064930 50.000 74 33 2 75 144 34 107 3.20e-13 67.4
MS.gene37894.t1 MTR_8g461260 43.137 102 45 3 75 163 34 135 3.37e-13 68.9
MS.gene37894.t1 MTR_2g064930 50.000 74 33 2 75 144 34 107 6.05e-13 67.4
MS.gene37894.t1 MTR_2g064930 50.000 74 33 2 75 144 34 107 7.43e-13 68.2
MS.gene37894.t1 MTR_2g103950 48.649 74 34 2 75 144 28 101 8.24e-13 67.8
MS.gene37894.t1 MTR_1g031780 40.698 86 47 2 63 144 28 113 1.13e-12 67.4
MS.gene37894.t1 MTR_1g098360 55.932 59 25 1 140 198 245 302 1.52e-12 67.0
MS.gene37894.t1 MTR_8g463990 44.048 84 43 2 61 140 18 101 2.66e-12 66.2
MS.gene37894.t1 MTR_5g037410 44.156 77 36 2 75 144 313 389 6.10e-12 65.5
MS.gene37894.t1 MTR_2g013210 45.455 77 38 2 73 145 35 111 7.05e-12 65.1
MS.gene37894.t1 MTR_5g075650 33.333 99 62 2 50 144 579 677 1.10e-11 65.1
MS.gene37894.t1 MTR_7g056680 33.333 126 75 5 31 150 286 408 1.23e-11 64.7
MS.gene37894.t1 MTR_1g102190 45.455 77 38 2 73 145 33 109 1.39e-11 64.3
MS.gene37894.t1 MTR_5g092120 41.176 85 44 2 72 150 55 139 1.42e-11 64.3
MS.gene37894.t1 MTR_0830s0010 32.000 150 70 5 76 195 677 824 1.58e-11 64.7
MS.gene37894.t1 MTR_3g062590 40.244 82 45 2 67 144 602 683 2.10e-11 64.3
MS.gene37894.t1 MTR_2g080100 50.000 70 30 3 74 140 456 523 2.69e-11 63.9
MS.gene37894.t1 MTR_2g080090 50.000 70 30 3 74 140 452 519 2.70e-11 63.9
MS.gene37894.t1 MTR_2g011240 39.130 92 52 2 57 144 473 564 3.69e-11 63.5
MS.gene37894.t1 MTR_1g110130 39.474 76 42 2 75 146 277 352 4.13e-11 63.2
MS.gene37894.t1 MTR_2g011160 29.323 133 74 4 32 144 456 588 4.39e-11 63.2
MS.gene37894.t1 MTR_2g011160 29.323 133 74 4 32 144 462 594 4.74e-11 63.2
MS.gene37894.t1 MTR_0194s0030 44.595 74 37 2 75 144 319 392 4.80e-11 63.2
MS.gene37894.t1 MTR_5g035030 47.368 76 36 3 140 213 208 281 5.04e-11 62.4
MS.gene37894.t1 MTR_8g083240 55.072 69 27 3 140 207 714 779 5.25e-11 63.2
MS.gene37894.t1 MTR_8g067630 44.156 77 38 2 73 144 275 351 5.48e-11 62.8
MS.gene37894.t1 MTR_5g055310 39.474 76 42 2 75 146 300 375 5.85e-11 62.8
MS.gene37894.t1 MTR_8g067930 44.156 77 38 2 73 144 327 403 6.55e-11 62.8
MS.gene37894.t1 MTR_2g100290 45.333 75 37 2 75 145 395 469 6.94e-11 62.4
MS.gene37894.t1 MTR_7g056680 34.167 120 70 5 31 144 447 563 7.97e-11 62.4
MS.gene37894.t1 MTR_1g105650 32.558 129 74 5 24 145 294 416 8.45e-11 62.4
MS.gene37894.t1 MTR_7g056680 34.167 120 70 5 31 144 442 558 8.57e-11 62.4
MS.gene37894.t1 MTR_2g011270 38.372 86 49 2 63 144 482 567 8.83e-11 62.4
MS.gene37894.t1 MTR_1g033000 53.030 66 27 3 140 204 723 785 9.44e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37894.t1 AT3G21630 56.842 95 41 0 50 144 285 379 2.97e-27 110
MS.gene37894.t1 AT3G21630 59.524 84 27 3 140 220 457 536 1.40e-17 82.4
MS.gene37894.t1 AT1G51940 43.902 82 46 0 69 150 323 404 1.91e-15 76.3
MS.gene37894.t1 AT1G18390 39.091 110 50 4 54 146 298 407 2.54e-13 69.7
MS.gene37894.t1 AT1G18390 39.091 110 50 4 54 146 292 401 2.94e-13 69.7
MS.gene37894.t1 AT3G26940 44.872 78 39 2 71 144 58 135 1.01e-12 67.8
MS.gene37894.t1 AT1G55200 48.649 74 34 2 75 144 367 440 1.41e-12 67.8
MS.gene37894.t1 AT1G55200 48.649 74 34 2 75 144 367 440 1.41e-12 67.8
MS.gene37894.t1 AT1G55200 48.649 74 34 2 75 144 367 440 1.41e-12 67.8
MS.gene37894.t1 AT1G55200 48.649 74 34 2 75 144 367 440 1.41e-12 67.8
MS.gene37894.t1 AT1G25390 42.105 76 40 2 75 146 279 354 1.47e-12 67.4
MS.gene37894.t1 AT1G25390 42.105 76 40 2 75 146 279 354 1.47e-12 67.4
MS.gene37894.t1 AT1G26150 43.038 79 41 2 70 144 413 491 2.46e-12 67.0
MS.gene37894.t1 AT4G23220 41.667 84 46 1 65 145 315 398 3.29e-12 66.6
MS.gene37894.t1 AT1G26150 43.038 79 41 2 70 144 413 491 3.45e-12 66.6
MS.gene37894.t1 AT1G20650 40.964 83 45 2 72 150 63 145 3.78e-12 65.9
MS.gene37894.t1 AT3G24540 47.297 74 35 2 75 144 171 244 4.56e-12 65.9
MS.gene37894.t1 AT1G29720 45.946 74 36 2 75 144 661 734 5.56e-12 65.9
MS.gene37894.t1 AT1G06840 38.095 84 48 2 66 145 604 687 6.30e-12 65.9
MS.gene37894.t1 AT1G49730 50.685 73 30 4 73 141 259 329 1.18e-11 64.7
MS.gene37894.t1 AT1G49730 50.685 73 30 4 73 141 315 385 1.21e-11 64.7
MS.gene37894.t1 AT1G49730 50.685 73 30 4 73 141 345 415 1.41e-11 64.3
MS.gene37894.t1 AT1G49730 50.685 73 30 4 73 141 315 385 1.55e-11 64.3
MS.gene37894.t1 AT4G13190 34.579 107 63 3 45 144 27 133 1.56e-11 64.3
MS.gene37894.t1 AT1G49730 50.685 73 30 4 73 141 345 415 1.58e-11 64.7
MS.gene37894.t1 AT1G56145 34.211 114 58 4 35 144 545 645 1.74e-11 64.3
MS.gene37894.t1 AT1G56145 34.211 114 58 4 35 144 648 748 1.84e-11 64.3
MS.gene37894.t1 AT4G13190 34.579 107 63 3 45 144 27 133 1.95e-11 63.5
MS.gene37894.t1 AT1G56145 34.211 114 58 4 35 144 648 748 1.99e-11 64.3
MS.gene37894.t1 AT5G42440 50.000 60 29 1 140 199 222 280 2.88e-11 63.2
MS.gene37894.t1 AT3G24550 44.595 74 37 2 75 144 268 341 3.01e-11 63.5
MS.gene37894.t1 AT5G07280 49.296 71 31 3 140 210 1059 1124 4.00e-11 63.5
MS.gene37894.t1 AT2G19230 40.217 92 42 3 75 155 559 648 4.53e-11 63.2
MS.gene37894.t1 AT2G19230 40.217 92 42 3 75 155 560 649 4.68e-11 63.2
MS.gene37894.t1 AT1G53420 41.892 74 39 2 75 144 612 685 4.83e-11 63.2
MS.gene37894.t1 AT4G21380 44.118 68 34 2 77 140 516 583 7.64e-11 62.4
MS.gene37894.t1 AT1G61400 31.356 118 71 3 31 144 450 561 8.97e-11 62.4
MS.gene37894.t1 AT1G29740 36.190 105 59 3 70 166 660 764 9.89e-11 62.4

Find 50 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACCGCAGGTTGCGGATTT+TGG 0.124929 5.3:+69678376 MS.gene37894:intron
TTCAGCATGGCTAATAAAAT+TGG 0.204108 5.3:+69677460 MS.gene37894:CDS
AAAATTGGTGAAGGTGGTTT+TGG 0.241672 5.3:+69677475 MS.gene37894:CDS
CAATTCAGCACAAAATTCTT+TGG 0.246303 5.3:-69677722 None:intergenic
AATGATGGTCCTCAAATAAA+AGG 0.269847 5.3:+69678697 MS.gene37894:CDS
TTTCACCTTCATCTTCTTGA+TGG 0.317835 5.3:-69677695 None:intergenic
TACCAACATCACAAGCATTA+TGG 0.363482 5.3:+69677384 MS.gene37894:CDS
TTTCACTTTACAGATCAAAA+TGG 0.374956 5.3:+69676911 MS.gene37894:CDS
AAGATGAACTCAGCTCAAAT+TGG 0.382640 5.3:+69677361 MS.gene37894:CDS
AGCCATGCTGAAATTATTTG+TGG 0.438697 5.3:-69677449 None:intergenic
AAAGTAGATGTCTACGCCTT+TGG 0.441237 5.3:+69678631 MS.gene37894:CDS
ACCACAATCGATCTCATACT+TGG 0.441882 5.3:-69679157 None:intergenic
TGGCAGTTTGATGAAGTGTT+TGG 0.445538 5.3:+69678913 MS.gene37894:intron
AATTTGAGCTGAGTTCATCT+TGG 0.446732 5.3:-69677359 None:intergenic
ATTTCCAACATCAGCAAGCT+TGG 0.458175 5.3:-69678404 None:intergenic
ATTACCAAAAGAAGAAGGTA+TGG 0.467761 5.3:+69677311 MS.gene37894:intron
GAATTTATACCTCGGGTGAA+GGG 0.491380 5.3:-69676945 None:intergenic
AGAATTTATACCTCGGGTGA+AGG 0.492510 5.3:-69676946 None:intergenic
CACCTTCATCTTCTTGATGG+CGG 0.496331 5.3:-69677692 None:intergenic
ATAGTTGCGGAGGGTACATT+CGG 0.498489 5.3:+69678442 MS.gene37894:CDS
CACCATAATGCTTGTGATGT+TGG 0.501300 5.3:-69677386 None:intergenic
GCTAATAAAATTGGTGAAGG+TGG 0.507666 5.3:+69677469 MS.gene37894:CDS
TTATCCAAGCTTGCTGATGT+TGG 0.510167 5.3:+69678400 MS.gene37894:CDS
AAGGCGTAGACATCTACTTT+CGG 0.511570 5.3:-69678628 None:intergenic
AATAAAAGGCCTTGTGGCTG+TGG 0.519442 5.3:+69678711 MS.gene37894:CDS
CTATGCAGAGCTGAGAGGCC+AGG 0.530086 5.3:+69677507 MS.gene37894:CDS
GACACTAACTTCTACAACTG+AGG 0.551851 5.3:+69679189 MS.gene37894:CDS
CTCATACTTGGACGTAGTCG+TGG 0.564459 5.3:-69679145 None:intergenic
AATTTATACCTCGGGTGAAG+GGG 0.566025 5.3:-69676944 None:intergenic
AAAGCAGCTGTAATCAATGA+TGG 0.571212 5.3:+69678682 MS.gene37894:CDS
AACTTCGTCAACTATAGTTG+CGG 0.583619 5.3:+69678429 MS.gene37894:CDS
TCCAAGTATGAGATCGATTG+TGG 0.583817 5.3:+69679156 MS.gene37894:CDS
AGCAAAAGCATGTACAATGA+GGG 0.589628 5.3:+69679120 MS.gene37894:CDS
TTCGTCAACTATAGTTGCGG+AGG 0.590388 5.3:+69678432 MS.gene37894:CDS
TAGCAAAAGCATGTACAATG+AGG 0.600715 5.3:+69679119 MS.gene37894:CDS
AGCCGCCATCAAGAAGATGA+AGG 0.602931 5.3:+69677690 MS.gene37894:CDS
TTACCAAAAGAAGAAGGTAT+GGG 0.609042 5.3:+69677312 MS.gene37894:intron
ATGGCTAATAAAATTGGTGA+AGG 0.610021 5.3:+69677466 MS.gene37894:CDS
TCTTGTGCATCACTTGAACC+TGG 0.612664 5.3:+69677756 MS.gene37894:CDS
CAACATCACAAGCATTATGG+TGG 0.619239 5.3:+69677387 MS.gene37894:CDS
GCTATTTGCCCCTTCACCCG+AGG 0.621610 5.3:+69676936 MS.gene37894:CDS
CGAGAATCACCACAGCCACA+AGG 0.639622 5.3:-69678720 None:intergenic
TCCTCAAATAAAAGGCCTTG+TGG 0.641440 5.3:+69678705 MS.gene37894:CDS
TGCCACAAATAATTTCAGCA+TGG 0.643522 5.3:+69677447 MS.gene37894:CDS
CTTATTTACAGATACGCATG+TGG 0.646342 5.3:+69678589 MS.gene37894:intron
GTCTTCTATGCAGAGCTGAG+AGG 0.646680 5.3:+69677502 MS.gene37894:CDS
GAGAATTGATCATACTCTGG+TGG 0.650261 5.3:-69678472 None:intergenic
TATGAGCAAGATCCAACAGA+AGG 0.650959 5.3:+69678937 MS.gene37894:CDS
TTCGTACAGAACAACACCAA+AGG 0.661515 5.3:-69678647 None:intergenic
TCGTCAACTATAGTTGCGGA+GGG 0.681793 5.3:+69678433 MS.gene37894:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTTATGTAAAATTATT+AGG - chr5.3:69678767-69678786 None:intergenic 10.0%
!!! AGTTTTTTTGTTAATTTATT+TGG + chr5.3:69678893-69678912 MS.gene37894:intron 10.0%
!! AAATCACTATTTATATTACT+AGG + chr5.3:69678813-69678832 MS.gene37894:intron 15.0%
!! TAGTAATATAAATAGTGATT+TGG - chr5.3:69678814-69678833 None:intergenic 15.0%
!! AAACTTGATGTAAATTAACA+TGG - chr5.3:69676983-69677002 None:intergenic 20.0%
!! AATAAAATTTCTTACACAAC+TGG - chr5.3:69679027-69679046 None:intergenic 20.0%
!! TAAATATTACCAAAAGAAGA+AGG + chr5.3:69677306-69677325 MS.gene37894:intron 20.0%
!! TAAATTAACATGGAGAATAA+GGG - chr5.3:69676973-69676992 None:intergenic 20.0%
!! TTCTCAAATTCTATATCTTA+AGG - chr5.3:69678523-69678542 None:intergenic 20.0%
!! TTTCAACATTAATAAAGGTT+TGG + chr5.3:69679065-69679084 MS.gene37894:intron 20.0%
!!! AAATAACCAACTTTTTTTGA+AGG - chr5.3:69677088-69677107 None:intergenic 20.0%
!!! AACGTTTTCAACATTAATAA+AGG + chr5.3:69679060-69679079 MS.gene37894:intron 20.0%
!!! TTTAATGTTTGCTATATTCA+TGG + chr5.3:69677865-69677884 MS.gene37894:intron 20.0%
!!! TTTTTACTGTTTTTCTTACT+GGG + chr5.3:69678322-69678341 MS.gene37894:intron 20.0%
!!! TTTTTTACTGTTTTTCTTAC+TGG + chr5.3:69678321-69678340 MS.gene37894:intron 20.0%
! AAAAACTTAACAAGCAAGAA+AGG - chr5.3:69678880-69678899 None:intergenic 25.0%
! ACATGAGATGAAAATAACAT+TGG - chr5.3:69677187-69677206 None:intergenic 25.0%
! ACATTGGCATTATAATGTTA+AGG - chr5.3:69677171-69677190 None:intergenic 25.0%
! ATGGGAGAAAAATTTGATTA+TGG + chr5.3:69677330-69677349 MS.gene37894:CDS 25.0%
! ATTCCTAAAGACTATATAGT+AGG - chr5.3:69677626-69677645 None:intergenic 25.0%
! ATTCCTACTATATAGTCTTT+AGG + chr5.3:69677620-69677639 MS.gene37894:intron 25.0%
! GTAAATTAACATGGAGAATA+AGG - chr5.3:69676974-69676993 None:intergenic 25.0%
! TTACAATGACATATTCACAT+CGG - chr5.3:69678091-69678110 None:intergenic 25.0%
! TTTCACTTTACAGATCAAAA+TGG + chr5.3:69676911-69676930 MS.gene37894:CDS 25.0%
!! AACAGAAGGTATAAAAAATC+TGG + chr5.3:69678951-69678970 MS.gene37894:intron 25.0%
!! TCTCGTTTATTCTTTTCAAT+TGG + chr5.3:69678738-69678757 MS.gene37894:intron 25.0%
!!! AGATTTTTTATACCTTCTGT+TGG - chr5.3:69678952-69678971 None:intergenic 25.0%
!!! ATTTTGATCTGTAAAGTGAA+AGG - chr5.3:69676912-69676931 None:intergenic 25.0%
AAAGAGAATTGATCATACTC+TGG - chr5.3:69678478-69678497 None:intergenic 30.0%
AATGATGGTCCTCAAATAAA+AGG + chr5.3:69678697-69678716 MS.gene37894:CDS 30.0%
AGACACATAAACTAATTACC+AGG - chr5.3:69677777-69677796 None:intergenic 30.0%
ATCTGCACAGTTGAAAAAAA+TGG - chr5.3:69679091-69679110 None:intergenic 30.0%
ATGAATATATTCAAGACCTC+TGG - chr5.3:69678195-69678214 None:intergenic 30.0%
ATGGCTAATAAAATTGGTGA+AGG + chr5.3:69677466-69677485 MS.gene37894:CDS 30.0%
ATTACCAAAAGAAGAAGGTA+TGG + chr5.3:69677311-69677330 MS.gene37894:intron 30.0%
CAATTCAGCACAAAATTCTT+TGG - chr5.3:69677725-69677744 None:intergenic 30.0%
CGTATCTGTAAATAAGGAAA+TGG - chr5.3:69678585-69678604 None:intergenic 30.0%
CTTACTCCTTCAAAAAAAGT+TGG + chr5.3:69677079-69677098 MS.gene37894:CDS 30.0%
GTCTATGAATACATTGACAA+TGG + chr5.3:69678001-69678020 MS.gene37894:intron 30.0%
GTGTTATAATTTACACGAAC+AGG + chr5.3:69677205-69677224 MS.gene37894:intron 30.0%
TAAGGGTAGAATTTATACCT+CGG - chr5.3:69676956-69676975 None:intergenic 30.0%
TCAATTCTCTTTCTCTTCAA+TGG + chr5.3:69678486-69678505 MS.gene37894:intron 30.0%
TGTCAATGTATTCATAGACA+AGG - chr5.3:69678000-69678019 None:intergenic 30.0%
TGTTAAGGATATCTTTACCT+TGG - chr5.3:69677156-69677175 None:intergenic 30.0%
TTAAACAGTCTCACATGATT+AGG - chr5.3:69677850-69677869 None:intergenic 30.0%
TTCAGCATGGCTAATAAAAT+TGG + chr5.3:69677460-69677479 MS.gene37894:CDS 30.0%
! AGAAGGTATAAAAAATCTGG+TGG + chr5.3:69678954-69678973 MS.gene37894:intron 30.0%
! CTTTAGGAATGCTTATATCA+TGG + chr5.3:69677636-69677655 MS.gene37894:intron 30.0%
! TTACCAAAAGAAGAAGGTAT+GGG + chr5.3:69677312-69677331 MS.gene37894:intron 30.0%
! TTTCTGATTTTATATCGCGA+TGG - chr5.3:69678241-69678260 None:intergenic 30.0%
!!! ATTTTTTTCAACTGTGCAGA+TGG + chr5.3:69679090-69679109 MS.gene37894:intron 30.0%
AAAGCAGCTGTAATCAATGA+TGG + chr5.3:69678682-69678701 MS.gene37894:CDS 35.0%
AACTTCGTCAACTATAGTTG+CGG + chr5.3:69678429-69678448 MS.gene37894:CDS 35.0%
AAGATGAACTCAGCTCAAAT+TGG + chr5.3:69677361-69677380 MS.gene37894:CDS 35.0%
AAGGGTAGAATTTATACCTC+GGG - chr5.3:69676955-69676974 None:intergenic 35.0%
AATTTGAGCTGAGTTCATCT+TGG - chr5.3:69677362-69677381 None:intergenic 35.0%
AGCCATGCTGAAATTATTTG+TGG - chr5.3:69677452-69677471 None:intergenic 35.0%
AGTCAAAATCTTCACGATTC+AGG + chr5.3:69678031-69678050 MS.gene37894:intron 35.0%
ATATCGCGATGGATATATAC+AGG - chr5.3:69678230-69678249 None:intergenic 35.0%
CAATGTCTATATCTAAGCCA+AGG + chr5.3:69677136-69677155 MS.gene37894:intron 35.0%
CACATGCGTATCTGTAAATA+AGG - chr5.3:69678591-69678610 None:intergenic 35.0%
CTTATTTACAGATACGCATG+TGG + chr5.3:69678589-69678608 MS.gene37894:intron 35.0%
GCTAATAAAATTGGTGAAGG+TGG + chr5.3:69677469-69677488 MS.gene37894:CDS 35.0%
GCTATTACTGGAATATCTGT+TGG + chr5.3:69677241-69677260 MS.gene37894:intron 35.0%
TAATTTACACGAACAGGTCT+AGG + chr5.3:69677211-69677230 MS.gene37894:intron 35.0%
TACCAACATCACAAGCATTA+TGG + chr5.3:69677384-69677403 MS.gene37894:CDS 35.0%
TGCCACAAATAATTTCAGCA+TGG + chr5.3:69677447-69677466 MS.gene37894:CDS 35.0%
TTGATTGGATATTGTGTCGA+AGG + chr5.3:69677965-69677984 MS.gene37894:intron 35.0%
TTTCACCTTCATCTTCTTGA+TGG - chr5.3:69677698-69677717 None:intergenic 35.0%
! AAAATTGGTGAAGGTGGTTT+TGG + chr5.3:69677475-69677494 MS.gene37894:CDS 35.0%
! AGACAAAAGCTTTTGTGCAA+AGG + chr5.3:69678271-69678290 MS.gene37894:intron 35.0%
! AGCAAAAGCATGTACAATGA+GGG + chr5.3:69679120-69679139 MS.gene37894:CDS 35.0%
! ATTTTCTTTTGCAGCACAGT+AGG + chr5.3:69677047-69677066 MS.gene37894:intron 35.0%
! CTCAATTGATTCAGTTTGCA+AGG + chr5.3:69678999-69679018 MS.gene37894:intron 35.0%
! TAGCAAAAGCATGTACAATG+AGG + chr5.3:69679119-69679138 MS.gene37894:CDS 35.0%
! TATCTGTTGGAGTATTAGCA+AGG + chr5.3:69677254-69677273 MS.gene37894:intron 35.0%
AAAGTAGATGTCTACGCCTT+TGG + chr5.3:69678631-69678650 MS.gene37894:CDS 40.0%
AACCTTTGTCATGGTCTACA+AGG + chr5.3:69678147-69678166 MS.gene37894:intron 40.0%
AAGGCGTAGACATCTACTTT+CGG - chr5.3:69678631-69678650 None:intergenic 40.0%
AATTTATACCTCGGGTGAAG+GGG - chr5.3:69676947-69676966 None:intergenic 40.0%
ACCACAATCGATCTCATACT+TGG - chr5.3:69679160-69679179 None:intergenic 40.0%
ACCATGTAGGTAGGTTTGAT+TGG + chr5.3:69677950-69677969 MS.gene37894:intron 40.0%
ACGTAATTGAGACCATGCAA+AGG - chr5.3:69677541-69677560 None:intergenic 40.0%
AGAATTTATACCTCGGGTGA+AGG - chr5.3:69676949-69676968 None:intergenic 40.0%
ATTTCCAACATCAGCAAGCT+TGG - chr5.3:69678407-69678426 None:intergenic 40.0%
CAACATCACAAGCATTATGG+TGG + chr5.3:69677387-69677406 MS.gene37894:CDS 40.0%
CAACCTATTTGTGACCATGT+AGG + chr5.3:69677937-69677956 MS.gene37894:intron 40.0%
CACCATAATGCTTGTGATGT+TGG - chr5.3:69677389-69677408 None:intergenic 40.0%
CAGAAAGAGAACCTTTGTCA+TGG + chr5.3:69678138-69678157 MS.gene37894:intron 40.0%
CATGACAAAGGTTCTCTTTC+TGG - chr5.3:69678140-69678159 None:intergenic 40.0%
CTACCTACATGGTCACAAAT+AGG - chr5.3:69677943-69677962 None:intergenic 40.0%
CTATTTGTGACCATGTAGGT+AGG + chr5.3:69677941-69677960 MS.gene37894:intron 40.0%
GAATTTATACCTCGGGTGAA+GGG - chr5.3:69676948-69676967 None:intergenic 40.0%
GAGAATTGATCATACTCTGG+TGG - chr5.3:69678475-69678494 None:intergenic 40.0%
GAGTAGTTATCTCCAAGTCT+AGG - chr5.3:69678982-69679001 None:intergenic 40.0%
TATGAGCAAGATCCAACAGA+AGG + chr5.3:69678937-69678956 MS.gene37894:CDS 40.0%
TCCAAGTATGAGATCGATTG+TGG + chr5.3:69679156-69679175 MS.gene37894:CDS 40.0%
TCCAATCAAACCTACCTACA+TGG - chr5.3:69677954-69677973 None:intergenic 40.0%
TCCTCAAATAAAAGGCCTTG+TGG + chr5.3:69678705-69678724 MS.gene37894:CDS 40.0%
TTCGTACAGAACAACACCAA+AGG - chr5.3:69678650-69678669 None:intergenic 40.0%
! TCTCCCATACCTTCTTCTTT+TGG - chr5.3:69677318-69677337 None:intergenic 40.0%
! TGGCAGTTTGATGAAGTGTT+TGG + chr5.3:69678913-69678932 MS.gene37894:intron 40.0%
!! TTATCCAAGCTTGCTGATGT+TGG + chr5.3:69678400-69678419 MS.gene37894:CDS 40.0%
AATCCAAAATCCGCAACCTG+CGG - chr5.3:69678382-69678401 None:intergenic 45.0%
CACCTTCATCTTCTTGATGG+CGG - chr5.3:69677695-69677714 None:intergenic 45.0%
CAGGTCTGTTATCCTTTGCA+TGG + chr5.3:69677526-69677545 MS.gene37894:intron 45.0%
CATGCAAAGGATAACAGACC+TGG - chr5.3:69677528-69677547 None:intergenic 45.0%
CTAGGAACTGTGGCTATTAC+TGG + chr5.3:69677229-69677248 MS.gene37894:intron 45.0%
TCGTCAACTATAGTTGCGGA+GGG + chr5.3:69678433-69678452 MS.gene37894:CDS 45.0%
TCTTGTGCATCACTTGAACC+TGG + chr5.3:69677756-69677775 MS.gene37894:CDS 45.0%
TTCGTCAACTATAGTTGCGG+AGG + chr5.3:69678432-69678451 MS.gene37894:CDS 45.0%
! AATAAAAGGCCTTGTGGCTG+TGG + chr5.3:69678711-69678730 MS.gene37894:CDS 45.0%
! AATCTGGTGGATCCTAGACT+TGG + chr5.3:69678967-69678986 MS.gene37894:intron 45.0%
! AGCCTTGTAGACCATGACAA+AGG - chr5.3:69678152-69678171 None:intergenic 45.0%
! ATAGTTGCGGAGGGTACATT+CGG + chr5.3:69678442-69678461 MS.gene37894:CDS 45.0%
! CATTATTCCTTTTGCACCGC+AGG + chr5.3:69678363-69678382 MS.gene37894:intron 45.0%
!! GCCACAAGGCCTTTTATTTG+AGG - chr5.3:69678709-69678728 None:intergenic 45.0%
ACGAACAGGTCTAGGAACTG+TGG + chr5.3:69677219-69677238 MS.gene37894:intron 50.0%
AGCCGCCATCAAGAAGATGA+AGG + chr5.3:69677690-69677709 MS.gene37894:CDS 50.0%
CTCATACTTGGACGTAGTCG+TGG - chr5.3:69679148-69679167 None:intergenic 50.0%
GTCTTCTATGCAGAGCTGAG+AGG + chr5.3:69677502-69677521 MS.gene37894:CDS 50.0%
TACAAGGCTGCAAATTGCGC+TGG + chr5.3:69678163-69678182 MS.gene37894:intron 50.0%
ATTGCGCTGGATTCAGCCAG+AGG + chr5.3:69678176-69678195 MS.gene37894:intron 55.0%
CGAGAATCACCACAGCCACA+AGG - chr5.3:69678723-69678742 None:intergenic 55.0%
! TCCTTTTGCACCGCAGGTTG+CGG + chr5.3:69678369-69678388 MS.gene37894:intron 55.0%
CTATGCAGAGCTGAGAGGCC+AGG + chr5.3:69677507-69677526 MS.gene37894:CDS 60.0%
GCTATTTGCCCCTTCACCCG+AGG + chr5.3:69676936-69676955 MS.gene37894:CDS 60.0%
TCCGCAACCTGCGGTGCAAA+AGG - chr5.3:69678373-69678392 None:intergenic 60.0%
! GCACCGCAGGTTGCGGATTT+TGG + chr5.3:69678376-69678395 MS.gene37894:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 69676904 69679209 69676904 ID=MS.gene37894
chr5.3 mRNA 69676904 69679209 69676904 ID=MS.gene37894.t1;Parent=MS.gene37894
chr5.3 exon 69676904 69676957 69676904 ID=MS.gene37894.t1.exon1;Parent=MS.gene37894.t1
chr5.3 CDS 69676904 69676957 69676904 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69677061 69677126 69677061 ID=MS.gene37894.t1.exon2;Parent=MS.gene37894.t1
chr5.3 CDS 69677061 69677126 69677061 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69677322 69677528 69677322 ID=MS.gene37894.t1.exon3;Parent=MS.gene37894.t1
chr5.3 CDS 69677322 69677528 69677322 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69677688 69677777 69677688 ID=MS.gene37894.t1.exon4;Parent=MS.gene37894.t1
chr5.3 CDS 69677688 69677777 69677688 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69678385 69678479 69678385 ID=MS.gene37894.t1.exon5;Parent=MS.gene37894.t1
chr5.3 CDS 69678385 69678479 69678385 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69678600 69678732 69678600 ID=MS.gene37894.t1.exon6;Parent=MS.gene37894.t1
chr5.3 CDS 69678600 69678732 69678600 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69678919 69678958 69678919 ID=MS.gene37894.t1.exon7;Parent=MS.gene37894.t1
chr5.3 CDS 69678919 69678958 69678919 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
chr5.3 exon 69679109 69679209 69679109 ID=MS.gene37894.t1.exon8;Parent=MS.gene37894.t1
chr5.3 CDS 69679109 69679209 69679109 ID=cds.MS.gene37894.t1;Parent=MS.gene37894.t1
Gene Sequence

>MS.gene37894

ATGGTCCTTTCACTTTACAGATCAAAATGGAAGCTATTTGCCCCTTCACCCGAGGTATAAATTCTACCCTTATTCTCCATGTTAATTTACATCAAGTTTTAATTTTTTTGACTTTATCTGTAAAAACATTATAATAAATTTTCATTTTCTTTTGCAGCACAGTAGGTTTGATCTTCTTACTCCTTCAAAAAAAGTTGGTTATTTGATTATTGTCTTTGCTGAAGTTTGATATCAATGTCTATATCTAAGCCAAGGTAAAGATATCCTTAACATTATAATGCCAATGTTATTTTCATCTCATGTGTTATAATTTACACGAACAGGTCTAGGAACTGTGGCTATTACTGGAATATCTGTTGGAGTATTAGCAAGGATTTTATTATTGTCATTGTTTGTGTATTTTAAATATTACCAAAAGAAGAAGGTATGGGAGAAAAATTTGATTATGGATGACTCCAAGATGAACTCAGCTCAAATTGGTACCAACATCACAAGCATTATGGTGGAAAAATCAGAAGAATTTTCATACAAAGAACTATCGATTGCCACAAATAATTTCAGCATGGCTAATAAAATTGGTGAAGGTGGTTTTGGAGAAGTCTTCTATGCAGAGCTGAGAGGCCAGGTCTGTTATCCTTTGCATGGTCTCAATTACGTAAAGCTTTCACTTTCTTTCTATTTTATTATCATAATGCATGTAAAACATGTGAGCAATTATTCCTACTATATAGTCTTTAGGAATGCTTATATCATGGTATTAAATAAGTCGTCTTGATTTACGCAGAAAGCCGCCATCAAGAAGATGAAGGTGAAAGCATCCAAAGAATTTTGTGCTGAATTGAAAGTCTTAACTCTTGTGCATCACTTGAACCTGGTAATTAGTTTATGTGTCTATTACTAAATATGATTGCTGTTTAAGAAACTAGAATTTAAAAGAAAAAGTCCTAATCATGTGAGACTGTTTAATGTTTGCTATATTCATGGATCATATTACACTGTTTTATATTATTATCTTTTACTTTCTTAATTTTATCAACCTATTTGTGACCATGTAGGTAGGTTTGATTGGATATTGTGTCGAAGGTTTTCTCTTCCTTGTCTATGAATACATTGACAATGGCAACTTAAGTCAAAATCTTCACGATTCAGGTGAGATAAACTCAACGAAAAATATATATGACGCACCGATGTGAATATGTCATTGTAAAGATTCTGATCAAGTAAAAAATGTTACCAGAAAGAGAACCTTTGTCATGGTCTACAAGGCTGCAAATTGCGCTGGATTCAGCCAGAGGTCTTGAATATATTCATGAACATACAGTACCTGTATATATCCATCGCGATATAAAATCAGAAAATATTTTATTAGACAAAAGCTTTTGTGCAAAGGTAGAAGTCGTATTACAATTTTTTTTATTTTTTTACTGTTTTTCTTACTGGGATTATGATGTGAATTCTTCATTATTCCTTTTGCACCGCAGGTTGCGGATTTTGGATTATCCAAGCTTGCTGATGTTGGAAATTCAACTTCGTCAACTATAGTTGCGGAGGGTACATTCGGTTACATGCCACCAGAGTATGATCAATTCTCTTTCTCTTCAATGGATCGCACTTTGCCTTAAGATATAGAATTTGAGAAAAAATGAGTTCATTTTTCATAAGTGTAAGATTGATGATTCCATTTCCTTATTTACAGATACGCATGTGGCAGTGTCTCTTCTTCTCCGAAAGTAGATGTCTACGCCTTTGGTGTTGTTCTGTACGAACTTACTTCTGCTAAAGCAGCTGTAATCAATGATGGTCCTCAAATAAAAGGCCTTGTGGCTGTGGTGATTCTCGTTTATTCTTTTCAATTGGCATCCTAATAATTTTACATAAAGATAAATGATGTTTGTTAAGACTGTATTCCAAATCACTATTTATATTACTAGGATATTTACTTGCATAAATAATTAAGCATAATGATACATTTTCCTTTCTTGCTTGTTAAGTTTTTTTGTTAATTTATTTGGCAGTTTGATGAAGTGTTTGGCTATGAGCAAGATCCAACAGAAGGTATAAAAAATCTGGTGGATCCTAGACTTGGAGATAACTACTCAATTGATTCAGTTTGCAAGGTACCAGTTGTGTAAGAAATTTTATTTTATTTTGATGATAACGTTTTCAACATTAATAAAGGTTTGGCCATTTTTTTCAACTGTGCAGATGGCACAACTAGCAAAAGCATGTACAATGAGGGATCCACGACTACGTCCAAGTATGAGATCGATTGTGGTTGCTCTTATGACACTAACTTCTACAACTGA
Protein sequence

>MS.gene37894.t1

MVLSLYRSKWKLFAPSPEHSRFDLLTPSKKVGYLIIVFAEKKVWEKNLIMDDSKMNSAQIGTNITSIMVEKSEEFSYKELSIATNNFSMANKIGEGGFGEVFYAELRGQKAAIKKMKVKASKEFCAELKVLTLVHHLNLVADFGLSKLADVGNSTSSTIVAEGTFGYMPPEYACGSVSSSPKVDVYAFGVVLYELTSAKAAVINDGPQIKGLVAVFDEVFGYEQDPTEDGTTSKSMYNEGSTTTSKYEIDCGCSYDTNFYN