AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37951

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37951.t1 MTR_5g095740 96.237 186 4 2 1 185 1 184 1.13e-130 364
MS.gene37951.t1 MTR_3g058980 75.258 194 35 5 1 185 1 190 2.53e-84 247
MS.gene37951.t1 MTR_2g026710 83.051 118 14 1 1 118 1 112 4.34e-67 202
MS.gene37951.t1 MTR_8g091720 74.074 135 31 2 10 140 13 147 3.80e-65 199
MS.gene37951.t1 MTR_1g072790 68.182 132 36 1 1 126 1 132 1.10e-61 189
MS.gene37951.t1 MTR_7g061270 66.906 139 38 2 22 160 25 155 4.85e-61 187
MS.gene37951.t1 MTR_7g100650 66.906 139 38 2 22 160 25 155 8.07e-61 187
MS.gene37951.t1 MTR_8g093920 85.567 97 14 0 22 118 39 135 5.69e-59 184
MS.gene37951.t1 MTR_4g133938 74.167 120 27 2 1 120 1 116 1.08e-58 180
MS.gene37951.t1 MTR_4g133952 66.990 103 34 0 16 118 51 153 4.50e-50 162
MS.gene37951.t1 MTR_1g088860 71.875 96 27 0 22 117 35 130 1.19e-49 159
MS.gene37951.t1 MTR_0392s0020 60.526 114 35 1 4 117 9 112 7.54e-48 152
MS.gene37951.t1 MTR_4g119500 67.010 97 32 0 22 118 21 117 8.00e-48 152
MS.gene37951.t1 MTR_1g039040 61.818 110 42 0 22 131 5 114 3.85e-47 152
MS.gene37951.t1 MTR_1g083070 53.279 122 48 2 23 135 4 125 1.14e-39 131
MS.gene37951.t1 MTR_1g029070 48.246 114 52 1 3 116 39 145 1.63e-29 107
MS.gene37951.t1 MTR_5g095900 47.368 95 47 1 20 111 10 104 1.48e-22 89.7
MS.gene37951.t1 MTR_1g029100 43.421 76 43 0 28 103 7 82 3.47e-19 78.2
MS.gene37951.t1 MTR_1g028480 45.349 86 43 1 53 134 19 104 4.94e-19 78.6
MS.gene37951.t1 MTR_2g056000 34.118 85 56 0 19 103 37 121 1.12e-15 71.6
MS.gene37951.t1 MTR_4g112380 36.250 80 51 0 24 103 12 91 1.23e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37951.t1 AT4G14540 86.777 121 14 1 1 121 1 119 2.31e-74 221
MS.gene37951.t1 AT5G47640 88.889 108 12 0 19 126 23 130 1.94e-67 204
MS.gene37951.t1 AT2G38880 70.732 123 36 0 4 126 2 124 3.66e-60 184
MS.gene37951.t1 AT2G38880 70.732 123 36 0 4 126 2 124 5.24e-60 184
MS.gene37951.t1 AT2G38880 70.732 123 36 0 4 126 2 124 5.24e-60 184
MS.gene37951.t1 AT2G38880 70.732 123 36 0 4 126 2 124 5.24e-60 184
MS.gene37951.t1 AT2G37060 68.462 130 39 1 6 135 15 142 3.99e-59 182
MS.gene37951.t1 AT2G37060 68.462 130 39 1 6 135 15 142 3.99e-59 182
MS.gene37951.t1 AT2G37060 68.462 130 39 1 6 135 15 142 3.99e-59 182
MS.gene37951.t1 AT3G53340 58.824 153 59 2 19 170 25 174 5.58e-59 182
MS.gene37951.t1 AT3G53340 58.824 153 59 2 19 170 25 174 5.58e-59 182
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 2.71e-57 176
MS.gene37951.t1 AT2G38880 69.919 123 35 1 4 126 2 122 4.82e-57 176
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 1.08e-56 176
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 1.08e-56 176
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 1.26e-56 176
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 1.26e-56 176
MS.gene37951.t1 AT2G38880 75.455 110 27 0 4 113 2 111 1.26e-56 176
MS.gene37951.t1 AT3G53340 80.000 95 19 0 19 113 25 119 1.49e-56 174
MS.gene37951.t1 AT3G53340 80.208 96 19 0 19 114 25 120 4.01e-56 174
MS.gene37951.t1 AT3G53340 80.208 96 19 0 19 114 25 120 4.01e-56 174
MS.gene37951.t1 AT2G13570 78.218 101 22 0 22 122 35 135 4.83e-56 176
MS.gene37951.t1 AT5G47670 49.693 163 74 2 6 160 12 174 4.50e-55 173
MS.gene37951.t1 AT5G47670 49.693 163 74 2 6 160 41 203 1.21e-54 173
MS.gene37951.t1 AT5G47670 49.693 163 74 2 6 160 41 203 1.21e-54 173
MS.gene37951.t1 AT2G47810 72.917 96 26 0 22 117 50 145 1.00e-49 158
MS.gene37951.t1 AT1G21970 63.366 101 37 0 22 122 58 158 1.15e-46 153
MS.gene37951.t1 AT1G09030 60.417 96 38 0 23 118 3 98 8.72e-42 137
MS.gene37951.t1 AT5G08190 38.272 81 50 0 23 103 11 91 7.75e-17 74.3
MS.gene37951.t1 AT5G08190 38.272 81 50 0 23 103 11 91 8.18e-17 74.3
MS.gene37951.t1 AT5G23090 37.179 78 49 0 24 101 12 89 2.36e-16 72.8
MS.gene37951.t1 AT5G23090 37.179 78 49 0 24 101 12 89 2.49e-16 72.8
MS.gene37951.t1 AT5G23090 37.179 78 49 0 24 101 12 89 2.49e-16 72.8
MS.gene37951.t1 AT5G23090 37.179 78 49 0 24 101 12 89 2.49e-16 72.8
MS.gene37951.t1 AT5G23090 35.938 64 41 0 40 103 15 78 5.59e-12 60.8

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCGCATTAGTTTCAGCATT+AGG 0.327218 5.1:-76486853 None:intergenic
TTTCTCTCTCTGACACTTAT+CGG 0.341007 5.1:-76486542 None:intergenic
AGTTTCAGCATTAGGATAAC+CGG 0.355328 5.1:-76486845 None:intergenic
AATAATGAAGAAGGCGTTAC+CGG 0.357624 5.1:+76486435 MS.gene37951:CDS
ATTCTGCTCACGTTTGCTAT+TGG 0.361435 5.1:-76486415 None:intergenic
CGGTGATAACTCGCTGTTCC+CGG 0.363693 5.1:-76486371 None:intergenic
CAGGGACACGTGTACGGTTC+TGG 0.366578 5.1:+76486796 MS.gene37951:CDS
AACTCGCTGTTCCCGGCGTT+CGG 0.373131 5.1:-76486364 None:intergenic
GGCTGATTCCGACAATGAAT+CGG 0.393443 5.1:+76486333 MS.gene37951:CDS
GCATCATCAGCATCAGCATC+AGG 0.396407 5.1:+76486777 MS.gene37951:CDS
TTCGAGATCTTCGCGTTCGC+CGG 0.412847 5.1:-76486454 None:intergenic
TCAACGGCGACGATCTACTT+TGG 0.416392 5.1:+76486575 MS.gene37951:CDS
CGGGAGGAGCACCGAACGCC+GGG 0.420650 5.1:+76486353 MS.gene37951:CDS
ACTAATAAGCTTGTTCTACC+TGG 0.429128 5.1:-76486881 None:intergenic
CGTTGAAGATAAATCTTCAA+TGG 0.432633 5.1:-76486634 None:intergenic
CATCAAGTGCCTGGTGGGCC+CGG 0.439513 5.1:+76486826 MS.gene37951:CDS
AGACGCGAAGGAAACAGTTC+AGG 0.459480 5.1:+76486477 MS.gene37951:CDS
CAACGGCGACGATCTACTTT+GGG 0.462526 5.1:+76486576 MS.gene37951:CDS
CTTTGGGCGATGACTACGTT+AGG 0.467469 5.1:+76486592 MS.gene37951:CDS
TGCTCCTCCCGATTCATTGT+CGG 0.482065 5.1:-76486341 None:intergenic
CGTTTGCTATTGGTAAGAAT+CGG 0.499147 5.1:-76486405 None:intergenic
GCTGATTCCGACAATGAATC+GGG 0.501404 5.1:+76486334 MS.gene37951:CDS
ACAGAAGACGAAGAAGAAGA+AGG 0.510989 5.1:-76486715 None:intergenic
ACACTTATCGGAAGCTTCGC+CGG 0.521889 5.1:-76486530 None:intergenic
ATCTTTATCACGTGCAGCAA+CGG 0.524172 5.1:-76486683 None:intergenic
TTAGGATAACCGGGCCCACC+AGG 0.536533 5.1:-76486835 None:intergenic
TATGGTGCTCCTCCTCAAGT+TGG 0.540598 5.1:+76486745 MS.gene37951:CDS
CCTAATGCTGAAACTAATGC+GGG 0.543861 5.1:+76486853 MS.gene37951:CDS
TCGGGAGGAGCACCGAACGC+CGG 0.558380 5.1:+76486352 MS.gene37951:CDS
CGTGAGCAGAATAATGAAGA+AGG 0.569587 5.1:+76486426 MS.gene37951:CDS
CAGCATCAGGGACACGTGTA+CGG 0.574817 5.1:+76486790 MS.gene37951:CDS
AACAGTTCAGGAATGTGTAT+CGG 0.575632 5.1:+76486489 MS.gene37951:CDS
GTTTCAGCATTAGGATAACC+GGG 0.577286 5.1:-76486844 None:intergenic
TCCTAATGCTGAAACTAATG+CGG 0.577317 5.1:+76486852 MS.gene37951:CDS
GAGAAACGAAAGACGATCAA+CGG 0.592030 5.1:+76486559 MS.gene37951:CDS
CTAATAAGCTTGTTCTACCT+GGG 0.593198 5.1:-76486880 None:intergenic
AAACTAATGCGGGTAGACCC+AGG 0.595538 5.1:+76486863 MS.gene37951:CDS
CATCATCAGCATCAGCATCA+GGG 0.606009 5.1:+76486778 MS.gene37951:CDS
GAGTTCATCAGCTTCATCAC+CGG 0.608726 5.1:+76486511 MS.gene37951:CDS
TGCATGATCATTCCAACTTG+AGG 0.615795 5.1:-76486757 None:intergenic
GGACACGTGTACGGTTCTGG+TGG 0.628725 5.1:+76486799 MS.gene37951:CDS
GAAGATCTCGAAAGACGCGA+AGG 0.642019 5.1:+76486465 MS.gene37951:CDS
AAGAATCGGTCTTGTTCTCG+CGG 0.644860 5.1:-76486391 None:intergenic
CAACGATTCAGAGAGATCGA+AGG 0.645752 5.1:+76486652 MS.gene37951:CDS
ATGATCATTCCAACTTGAGG+AGG 0.661433 5.1:-76486754 None:intergenic
GTTGCTGCACGTGATAAAGA+TGG 0.679086 5.1:+76486685 MS.gene37951:CDS
GATTCCGACAATGAATCGGG+AGG 0.690680 5.1:+76486337 MS.gene37951:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CGTTGAAGATAAATCTTCAA+TGG - chr5.1:76486637-76486656 None:intergenic 30.0%
AACAGTTCAGGAATGTGTAT+CGG + chr5.1:76486489-76486508 MS.gene37951:CDS 35.0%
AGTTTCAGCATTAGGATAAC+CGG - chr5.1:76486848-76486867 None:intergenic 35.0%
TCCTAATGCTGAAACTAATG+CGG + chr5.1:76486852-76486871 MS.gene37951:CDS 35.0%
TTTCTCTCTCTGACACTTAT+CGG - chr5.1:76486545-76486564 None:intergenic 35.0%
! TCGTCTTCTGTTTTCGATTA+TGG + chr5.1:76486727-76486746 MS.gene37951:CDS 35.0%
!! AATAATGAAGAAGGCGTTAC+CGG + chr5.1:76486435-76486454 MS.gene37951:CDS 35.0%
!! CGTTTGCTATTGGTAAGAAT+CGG - chr5.1:76486408-76486427 None:intergenic 35.0%
ACAGAAGACGAAGAAGAAGA+AGG - chr5.1:76486718-76486737 None:intergenic 40.0%
ATCTTTATCACGTGCAGCAA+CGG - chr5.1:76486686-76486705 None:intergenic 40.0%
ATGATCATTCCAACTTGAGG+AGG - chr5.1:76486757-76486776 None:intergenic 40.0%
CCTAATGCTGAAACTAATGC+GGG + chr5.1:76486853-76486872 MS.gene37951:CDS 40.0%
CGTGAGCAGAATAATGAAGA+AGG + chr5.1:76486426-76486445 MS.gene37951:CDS 40.0%
GAGAAACGAAAGACGATCAA+CGG + chr5.1:76486559-76486578 MS.gene37951:CDS 40.0%
GTTTCAGCATTAGGATAACC+GGG - chr5.1:76486847-76486866 None:intergenic 40.0%
TGCATGATCATTCCAACTTG+AGG - chr5.1:76486760-76486779 None:intergenic 40.0%
!! ATTCTGCTCACGTTTGCTAT+TGG - chr5.1:76486418-76486437 None:intergenic 40.0%
CAACGATTCAGAGAGATCGA+AGG + chr5.1:76486652-76486671 MS.gene37951:CDS 45.0%
CATCATCAGCATCAGCATCA+GGG + chr5.1:76486778-76486797 MS.gene37951:CDS 45.0%
GAGTTCATCAGCTTCATCAC+CGG + chr5.1:76486511-76486530 MS.gene37951:CDS 45.0%
GTTGCTGCACGTGATAAAGA+TGG + chr5.1:76486685-76486704 MS.gene37951:CDS 45.0%
! CCCGCATTAGTTTCAGCATT+AGG - chr5.1:76486856-76486875 None:intergenic 45.0%
! GCTGATTCCGACAATGAATC+GGG + chr5.1:76486334-76486353 MS.gene37951:CDS 45.0%
! GGCTGATTCCGACAATGAAT+CGG + chr5.1:76486333-76486352 MS.gene37951:CDS 45.0%
! GTTTTCATCAAGTGCCTGGT+GGG + chr5.1:76486821-76486840 MS.gene37951:CDS 45.0%
!! AAGAATCGGTCTTGTTCTCG+CGG - chr5.1:76486394-76486413 None:intergenic 45.0%
AAACTAATGCGGGTAGACCC+AGG + chr5.1:76486863-76486882 MS.gene37951:CDS 50.0%
ACACTTATCGGAAGCTTCGC+CGG - chr5.1:76486533-76486552 None:intergenic 50.0%
AGACGCGAAGGAAACAGTTC+AGG + chr5.1:76486477-76486496 MS.gene37951:CDS 50.0%
CAACGGCGACGATCTACTTT+GGG + chr5.1:76486576-76486595 MS.gene37951:CDS 50.0%
CTTTGGGCGATGACTACGTT+AGG + chr5.1:76486592-76486611 MS.gene37951:CDS 50.0%
GAAGATCTCGAAAGACGCGA+AGG + chr5.1:76486465-76486484 MS.gene37951:CDS 50.0%
GATTCCGACAATGAATCGGG+AGG + chr5.1:76486337-76486356 MS.gene37951:CDS 50.0%
GCATCATCAGCATCAGCATC+AGG + chr5.1:76486777-76486796 MS.gene37951:CDS 50.0%
TATGGTGCTCCTCCTCAAGT+TGG + chr5.1:76486745-76486764 MS.gene37951:CDS 50.0%
TCAACGGCGACGATCTACTT+TGG + chr5.1:76486575-76486594 MS.gene37951:CDS 50.0%
TGCTCCTCCCGATTCATTGT+CGG - chr5.1:76486344-76486363 None:intergenic 50.0%
! GGTGGTTTTCATCAAGTGCC+TGG + chr5.1:76486817-76486836 MS.gene37951:CDS 50.0%
! GGTTTTCATCAAGTGCCTGG+TGG + chr5.1:76486820-76486839 MS.gene37951:CDS 50.0%
CAGCATCAGGGACACGTGTA+CGG + chr5.1:76486790-76486809 MS.gene37951:CDS 55.0%
CGGTGATAACTCGCTGTTCC+CGG - chr5.1:76486374-76486393 None:intergenic 55.0%
TTCGAGATCTTCGCGTTCGC+CGG - chr5.1:76486457-76486476 None:intergenic 55.0%
AACTCGCTGTTCCCGGCGTT+CGG - chr5.1:76486367-76486386 None:intergenic 60.0%
CAGGGACACGTGTACGGTTC+TGG + chr5.1:76486796-76486815 MS.gene37951:CDS 60.0%
TTAGGATAACCGGGCCCACC+AGG - chr5.1:76486838-76486857 None:intergenic 60.0%
! GGACACGTGTACGGTTCTGG+TGG + chr5.1:76486799-76486818 MS.gene37951:CDS 60.0%
! CATCAAGTGCCTGGTGGGCC+CGG + chr5.1:76486826-76486845 MS.gene37951:CDS 65.0%
!! TCGGGAGGAGCACCGAACGC+CGG + chr5.1:76486352-76486371 MS.gene37951:CDS 70.0%
!! CGGGAGGAGCACCGAACGCC+GGG + chr5.1:76486353-76486372 MS.gene37951:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 76486331 76486888 76486331 ID=MS.gene37951
chr5.1 mRNA 76486331 76486888 76486331 ID=MS.gene37951.t1;Parent=MS.gene37951
chr5.1 exon 76486331 76486888 76486331 ID=MS.gene37951.t1.exon1;Parent=MS.gene37951.t1
chr5.1 CDS 76486331 76486888 76486331 ID=cds.MS.gene37951.t1;Parent=MS.gene37951.t1
Gene Sequence

>MS.gene37951

ATGGCTGATTCCGACAATGAATCGGGAGGAGCACCGAACGCCGGGAACAGCGAGTTATCACCGCGAGAACAAGACCGATTCTTACCAATAGCAAACGTGAGCAGAATAATGAAGAAGGCGTTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAGGAAACAGTTCAGGAATGTGTATCGGAGTTCATCAGCTTCATCACCGGCGAAGCTTCCGATAAGTGTCAGAGAGAGAAACGAAAGACGATCAACGGCGACGATCTACTTTGGGCGATGACTACGTTAGGATTTGAAGAGTACGTTGAACCATTGAAGATTTATCTTCAACGATTCAGAGAGATCGAAGGTGAGAAAACCGTTGCTGCACGTGATAAAGATGGTGTTGCTCCTTCTTCTTCTTCGTCTTCTGTTTTCGATTATGGTGCTCCTCCTCAAGTTGGAATGATCATGCATCATCAGCATCAGCATCAGGGACACGTGTACGGTTCTGGTGGTTTTCATCAAGTGCCTGGTGGGCCCGGTTATCCTAATGCTGAAACTAATGCGGGTAGACCCAGGTAG
Protein sequence

>MS.gene37951.t1

MADSDNESGGAPNAGNSELSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKIYLQRFREIEGEKTVAARDKDGVAPSSSSSSVFDYGAPPQVGMIMHHQHQHQGHVYGSGGFHQVPGGPGYPNAETNAGRPR