AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38003


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38003.t1 MTR_5g096700 94.545 330 16 1 1 328 1 330 0.0 647
MS.gene38003.t1 MTR_5g096700 94.096 271 16 0 58 328 1 271 0.0 528
MS.gene38003.t1 MTR_3g061090 63.526 329 117 3 1 327 1 328 1.31e-150 426
MS.gene38003.t1 MTR_3g061090 64.981 257 87 3 73 327 1 256 1.94e-115 334
MS.gene38003.t1 MTR_1g029850 44.308 325 176 3 7 327 5 328 1.46e-97 291
MS.gene38003.t1 MTR_3g088560 45.963 322 169 4 5 323 2 321 1.29e-96 289
MS.gene38003.t1 MTR_5g096790 45.619 331 153 10 6 323 6 322 9.84e-89 269
MS.gene38003.t1 MTR_3g088560 46.025 239 125 3 88 323 14 251 5.01e-66 208
MS.gene38003.t1 MTR_3g088560 45.992 237 124 3 90 323 31 266 2.41e-65 207
MS.gene38003.t1 MTR_3g088560 46.465 198 102 3 129 323 3 199 8.22e-53 172
MS.gene38003.t1 MTR_5g005940 29.787 141 96 3 163 302 536 674 6.33e-13 70.1
MS.gene38003.t1 MTR_3g072160 33.537 164 105 3 133 292 33 196 1.29e-12 67.0
MS.gene38003.t1 MTR_3g072160 33.537 164 105 3 133 292 70 233 1.30e-12 67.4
MS.gene38003.t1 MTR_7g094900 29.231 130 90 2 163 292 545 672 2.70e-12 68.2
MS.gene38003.t1 MTR_7g094910 29.231 130 90 2 163 292 526 653 2.75e-12 67.8
MS.gene38003.t1 MTR_7g094490 28.000 150 96 3 153 291 197 345 2.85e-12 67.4
MS.gene38003.t1 MTR_1g072650 27.815 151 97 3 153 292 201 350 9.29e-12 65.9
MS.gene38003.t1 MTR_3g088660 28.289 152 103 3 174 323 94 241 1.07e-11 64.3
MS.gene38003.t1 MTR_5g087500 29.487 156 90 4 153 292 191 342 1.41e-11 65.5
MS.gene38003.t1 MTR_5g087500 29.487 156 90 4 153 292 191 342 1.59e-11 65.1
MS.gene38003.t1 MTR_5g087500 30.065 153 87 4 153 289 191 339 1.64e-11 64.7
MS.gene38003.t1 MTR_7g086500 29.139 151 91 4 153 289 178 326 3.51e-11 64.3
MS.gene38003.t1 MTR_7g086500 29.139 151 91 4 153 289 178 326 3.53e-11 63.9
MS.gene38003.t1 MTR_3g069000 25.000 160 108 2 142 290 37 195 5.05e-11 62.8
MS.gene38003.t1 MTR_1g104870 29.771 131 88 3 163 292 539 666 8.65e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38003.t1 AT1G55760 57.012 328 139 2 1 327 1 327 1.38e-136 390
MS.gene38003.t1 AT1G21780 47.516 322 166 3 7 327 5 324 4.61e-108 318
MS.gene38003.t1 AT1G21780 47.516 322 166 3 7 327 5 324 4.61e-108 318
MS.gene38003.t1 AT1G01640 29.787 188 128 4 142 327 3 188 4.33e-15 73.6
MS.gene38003.t1 AT2G39760 28.947 152 96 2 151 291 182 332 9.46e-14 71.6
MS.gene38003.t1 AT2G39760 28.947 152 96 2 151 291 182 332 1.37e-13 71.2
MS.gene38003.t1 AT2G39760 28.947 152 96 2 151 291 182 332 1.37e-13 71.2
MS.gene38003.t1 AT2G39760 28.947 152 96 2 151 291 182 332 2.40e-13 70.9
MS.gene38003.t1 AT3G56230 29.221 154 106 3 142 292 90 243 4.41e-13 68.9
MS.gene38003.t1 AT1G01640 29.586 169 115 4 161 327 1 167 4.91e-13 67.0
MS.gene38003.t1 AT1G05690 26.415 159 108 4 149 301 40 195 2.13e-11 64.7
MS.gene38003.t1 AT5G19330 28.462 130 91 2 163 292 541 668 4.67e-11 64.3
MS.gene38003.t1 AT3G06190 27.632 152 98 3 151 291 190 340 7.15e-11 63.2
MS.gene38003.t1 AT2G30600 28.671 143 97 3 138 275 326 468 8.33e-11 63.5
MS.gene38003.t1 AT2G30600 28.671 143 97 3 138 275 326 468 8.33e-11 63.5
MS.gene38003.t1 AT2G30600 28.671 143 97 3 138 275 326 468 8.33e-11 63.5
MS.gene38003.t1 AT2G30600 28.671 143 97 3 138 275 326 468 8.33e-11 63.5

Find 44 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAATATCTTGTGAAATT+TGG 0.204904 5.1:+77055426 MS.gene38003:CDS
TGGCAAAATATATGATATTC+AGG 0.211529 5.1:+77055446 MS.gene38003:CDS
ATTGATACAAAGTTGCAATT+TGG 0.261172 5.1:-77055380 None:intergenic
GTGGGGAAGCTTGCTCTATT+TGG 0.265550 5.1:+77054953 MS.gene38003:CDS
CCATTGAACTAGACGATTCA+TGG 0.288711 5.1:-77054182 None:intergenic
CAATTGCATCTTTCATAGTT+AGG 0.300214 5.1:+77054435 MS.gene38003:CDS
GCTTGCTCTATTTGGCCTAA+TGG 0.324580 5.1:+77054961 MS.gene38003:CDS
AGAATTTCTTATACATAGAT+TGG 0.343658 5.1:+77055254 MS.gene38003:CDS
ACTTGTCAGCTGCATGTAAT+AGG 0.350791 5.1:-77055278 None:intergenic
AGCAAAATCTTGACCCAATC+AGG 0.352906 5.1:+77054479 MS.gene38003:CDS
ATAGCTTTCTGTGGGAGATT+GGG 0.358877 5.1:+77054659 MS.gene38003:CDS
GATAGCTTTCTGTGGGAGAT+TGG 0.359309 5.1:+77054658 MS.gene38003:CDS
TCAAAGAAATTCTTGATGCT+TGG 0.368629 5.1:+77055523 MS.gene38003:CDS
AGATAAACTTCTTAGCAATA+AGG 0.369571 5.1:+77054636 MS.gene38003:CDS
GTTAGGATTCTTAGTTATGC+TGG 0.386999 5.1:+77054452 MS.gene38003:CDS
AGGGTTGTGTGTGATTGTTT+TGG 0.394454 5.1:-77054411 None:intergenic
GCCATAAAGAAAATTTATAA+AGG 0.401897 5.1:-77055217 None:intergenic
TCAAAATAGCCATGTGGAAC+TGG 0.403978 5.1:+77054251 MS.gene38003:CDS
TTCTCTTATCATTCGACCTA+GGG 0.404879 5.1:-77055022 None:intergenic
CTAACTATGAAAGATGCAAT+TGG 0.440177 5.1:-77054434 None:intergenic
CCATGAATCGTCTAGTTCAA+TGG 0.453483 5.1:+77054182 MS.gene38003:CDS
TCATTCGACCTAGGGATTCA+AGG 0.458286 5.1:-77055014 None:intergenic
TAGAACTTGAATCACCTGAT+TGG 0.468839 5.1:-77054493 None:intergenic
AGAACTTGAATCACCTGATT+GGG 0.473517 5.1:-77054492 None:intergenic
CTCTGCTGCATGAATCCATT+AGG 0.476375 5.1:-77054976 None:intergenic
TAGCTTTCTGTGGGAGATTG+GGG 0.476737 5.1:+77054660 MS.gene38003:CDS
GAGATTGGGGTTCCACTACC+TGG 0.484202 5.1:+77054673 MS.gene38003:CDS
CTTCTCTTATCATTCGACCT+AGG 0.496175 5.1:-77055023 None:intergenic
GGCAAAATATATGATATTCA+GGG 0.516583 5.1:+77055447 MS.gene38003:CDS
CATTCGACCTAGGGATTCAA+GGG 0.532711 5.1:-77055013 None:intergenic
ATGAGCTCTTATGCTTCTCT+CGG 0.539209 5.1:-77055079 None:intergenic
CAATAAGGATAGCTTTCTGT+GGG 0.562302 5.1:+77054651 MS.gene38003:CDS
ACTATGAAAGATGCAATTGG+AGG 0.571379 5.1:-77054431 None:intergenic
GCAGCGGCCCTTGAATCCCT+AGG 0.588324 5.1:+77055006 MS.gene38003:CDS
GCAATAAGGATAGCTTTCTG+TGG 0.597072 5.1:+77054650 MS.gene38003:CDS
TTTGAAAACTACATGTCCAA+AGG 0.597428 5.1:+77054726 MS.gene38003:CDS
CTATGAAAGATGCAATTGGA+GGG 0.614089 5.1:-77054430 None:intergenic
GATATGAAGGCATTTATCAG+TGG 0.615660 5.1:+77054346 MS.gene38003:intron
TTGACTTTACCAGTTCCACA+TGG 0.646739 5.1:-77054260 None:intergenic
GAAAACATGCTGCGAAACAC+AGG 0.659506 5.1:-77055126 None:intergenic
TTATCAGTGGAAAAGAACTG+TGG 0.666641 5.1:+77054359 MS.gene38003:CDS
AAAAGATTGAATTGACATGT+CGG 0.667795 5.1:-77055190 None:intergenic
GCAGCAGAGACAAAATGCAG+CGG 0.684257 5.1:+77054990 MS.gene38003:CDS
ATCCATTCAAAATAGCCATG+TGG 0.702367 5.1:+77054245 MS.gene38003:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAATATATCCAAATTA+AGG + chr5.1:77054757-77054776 MS.gene38003:intron 10.0%
!! CAAACAAAATAAAAAGTAAA+TGG - chr5.1:77054822-77054841 None:intergenic 15.0%
!! TATTATTCAATACCTTAATT+TGG - chr5.1:77054772-77054791 None:intergenic 15.0%
!!! ACCTTTATAAATTTTCTTTA+TGG + chr5.1:77055216-77055235 MS.gene38003:CDS 15.0%
!!! ATCAAGGAAAATTTTAAATT+AGG - chr5.1:77054922-77054941 None:intergenic 15.0%
!!! CATTTACTTTTTATTTTGTT+TGG + chr5.1:77054820-77054839 MS.gene38003:intron 15.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5.1:77055254-77055273 MS.gene38003:CDS 20.0%
!! ATTATATACTTACATACCTT+TGG - chr5.1:77054745-77054764 None:intergenic 20.0%
!! GCCATAAAGAAAATTTATAA+AGG - chr5.1:77055220-77055239 None:intergenic 20.0%
!!! ATAATCATAGTATTGATATG+AGG + chr5.1:77054873-77054892 MS.gene38003:intron 20.0%
!!! ATGTCAATTATAAATTTTCC+AGG - chr5.1:77054694-77054713 None:intergenic 20.0%
!!! TAATCATAGTATTGATATGA+GGG + chr5.1:77054874-77054893 MS.gene38003:intron 20.0%
! AAAAGATTGAATTGACATGT+CGG - chr5.1:77055193-77055212 None:intergenic 25.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5.1:77054636-77054655 MS.gene38003:CDS 25.0%
! ATGCAATATCTTGTGAAATT+TGG + chr5.1:77055426-77055445 MS.gene38003:CDS 25.0%
! ATTGATACAAAGTTGCAATT+TGG - chr5.1:77055383-77055402 None:intergenic 25.0%
! GGCAAAATATATGATATTCA+GGG + chr5.1:77055447-77055466 MS.gene38003:CDS 25.0%
! TGGCAAAATATATGATATTC+AGG + chr5.1:77055446-77055465 MS.gene38003:CDS 25.0%
!! ATTATAAATTTTCCAGGTAG+TGG - chr5.1:77054688-77054707 None:intergenic 25.0%
!!! ATTTTCCTTGATTTGAATGA+AGG + chr5.1:77054930-77054949 MS.gene38003:intron 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5.1:77054333-77054352 MS.gene38003:intron 25.0%
!!! TGATATTTTTGAGTTAAGAG+AGG + chr5.1:77055302-77055321 MS.gene38003:CDS 25.0%
!!! TTTTCCTTGATTTGAATGAA+GGG + chr5.1:77054931-77054950 MS.gene38003:intron 25.0%
CAATTGCATCTTTCATAGTT+AGG + chr5.1:77054435-77054454 MS.gene38003:CDS 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5.1:77054437-77054456 None:intergenic 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5.1:77055523-77055542 MS.gene38003:CDS 30.0%
TTTGAAAACTACATGTCCAA+AGG + chr5.1:77054726-77054745 MS.gene38003:CDS 30.0%
! CATGTTTTCACATAATGTCA+AGG + chr5.1:77055140-77055159 MS.gene38003:CDS 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5.1:77054618-77054637 None:intergenic 30.0%
!!! TGTTTGGTGTTGATTTTTGA+AGG + chr5.1:77054836-77054855 MS.gene38003:intron 30.0%
ACTATGAAAGATGCAATTGG+AGG - chr5.1:77054434-77054453 None:intergenic 35.0%
AGAACTTGAATCACCTGATT+GGG - chr5.1:77054495-77054514 None:intergenic 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5.1:77054245-77054264 MS.gene38003:CDS 35.0%
CAATAAGGATAGCTTTCTGT+GGG + chr5.1:77054651-77054670 MS.gene38003:CDS 35.0%
CTATGAAAGATGCAATTGGA+GGG - chr5.1:77054433-77054452 None:intergenic 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5.1:77054452-77054471 MS.gene38003:CDS 35.0%
TAGAACTTGAATCACCTGAT+TGG - chr5.1:77054496-77054515 None:intergenic 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5.1:77054359-77054378 MS.gene38003:CDS 35.0%
TTCTCTTATCATTCGACCTA+GGG - chr5.1:77055025-77055044 None:intergenic 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5.1:77054346-77054365 MS.gene38003:intron 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5.1:77054250-77054269 None:intergenic 35.0%
!!! GTGATTGTTTTGGACAATTC+TGG - chr5.1:77054404-77054423 None:intergenic 35.0%
ACTTGTCAGCTGCATGTAAT+AGG - chr5.1:77055281-77055300 None:intergenic 40.0%
AGCAAAATCTTGACCCAATC+AGG + chr5.1:77054479-77054498 MS.gene38003:CDS 40.0%
ATAGCTTTCTGTGGGAGATT+GGG + chr5.1:77054659-77054678 MS.gene38003:CDS 40.0%
ATGAGCTCTTATGCTTCTCT+CGG - chr5.1:77055082-77055101 None:intergenic 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5.1:77054185-77054204 None:intergenic 40.0%
CTTCTCTTATCATTCGACCT+AGG - chr5.1:77055026-77055045 None:intergenic 40.0%
GCAATAAGGATAGCTTTCTG+TGG + chr5.1:77054650-77054669 MS.gene38003:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5.1:77054251-77054270 MS.gene38003:CDS 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5.1:77054263-77054282 None:intergenic 40.0%
! AGGGTTGTGTGTGATTGTTT+TGG - chr5.1:77054414-77054433 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5.1:77054182-77054201 MS.gene38003:CDS 40.0%
! CCTTGATTTGAATGAAGGGT+GGG + chr5.1:77054935-77054954 MS.gene38003:intron 40.0%
! CTTGATTTGAATGAAGGGTG+GGG + chr5.1:77054936-77054955 MS.gene38003:intron 40.0%
! TCCTTGATTTGAATGAAGGG+TGG + chr5.1:77054934-77054953 MS.gene38003:intron 40.0%
CATTCGACCTAGGGATTCAA+GGG - chr5.1:77055016-77055035 None:intergenic 45.0%
CCCACCCTTCATTCAAATCA+AGG - chr5.1:77054938-77054957 None:intergenic 45.0%
CTCTGCTGCATGAATCCATT+AGG - chr5.1:77054979-77054998 None:intergenic 45.0%
GAAAACATGCTGCGAAACAC+AGG - chr5.1:77055129-77055148 None:intergenic 45.0%
GATAGCTTTCTGTGGGAGAT+TGG + chr5.1:77054658-77054677 MS.gene38003:CDS 45.0%
GCTTGCTCTATTTGGCCTAA+TGG + chr5.1:77054961-77054980 MS.gene38003:CDS 45.0%
TAGCTTTCTGTGGGAGATTG+GGG + chr5.1:77054660-77054679 MS.gene38003:CDS 45.0%
TCATTCGACCTAGGGATTCA+AGG - chr5.1:77055017-77055036 None:intergenic 45.0%
GCAGCAGAGACAAAATGCAG+CGG + chr5.1:77054990-77055009 MS.gene38003:CDS 50.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5.1:77054953-77054972 MS.gene38003:CDS 50.0%
GAGATTGGGGTTCCACTACC+TGG + chr5.1:77054673-77054692 MS.gene38003:CDS 55.0%
GCAGCGGCCCTTGAATCCCT+AGG + chr5.1:77055006-77055025 MS.gene38003:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 77054154 77055554 77054154 ID=MS.gene38003
chr5.1 mRNA 77054154 77055554 77054154 ID=MS.gene38003.t1;Parent=MS.gene38003
chr5.1 exon 77054154 77054272 77054154 ID=MS.gene38003.t1.exon1;Parent=MS.gene38003.t1
chr5.1 CDS 77054154 77054272 77054154 ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1 exon 77054355 77054500 77054355 ID=MS.gene38003.t1.exon2;Parent=MS.gene38003.t1
chr5.1 CDS 77054355 77054500 77054355 ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1 exon 77054629 77054747 77054629 ID=MS.gene38003.t1.exon3;Parent=MS.gene38003.t1
chr5.1 CDS 77054629 77054747 77054629 ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1 exon 77054952 77055554 77054952 ID=MS.gene38003.t1.exon4;Parent=MS.gene38003.t1
chr5.1 CDS 77054952 77055554 77054952 ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
Gene Sequence

>MS.gene38003

ATGAATGATTATGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGTAAAGTCAAGTTTACAATTTTAATATTTTTTAGTATTATATTTGTTTACATATTGCTAAGTTTTGTTCTTATGATATGAAGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAATTGTCCAAAACAATCACACACAACCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCTGGAAATAGCAAAATCTTGACCCAATCAGGTGATTCAAGTTCTATGTTTAAAAAAGTATATAATTTAGTTAACACGAATAGTATTTGATTTAACAAGTTAGCTATAGTATTAATATGTGTGTTGATTTTGATCTTTATCTTTTCCACACTGATGTAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTCTGTGGGAGATTGGGGTTCCACTACCTGGAAAATTTATAATTGACATTGAGTTTCTTGATTTGAAAACTACATGTCCAAAGGTATGTAAGTATATAATATATCCAAATTAAGGTATTGAATAATATATGTAAACATTGATACAATTTTTTTTCCATTTACTTTTTATTTTGTTTGGTGTTGATTTTTGAAGGACTAGTCATGAAGCATAATCATAGTATTGATATGAGGGTTATTGACTTACAATTTGTTCACCTAATTTAAAATTTTCCTTGATTTGAATGAAGGGTGGGGAAGCTTGCTCTATTTGGCCTAATGGATTCATGCAGCAGAGACAAAATGCAGCGGCCCTTGAATCCCTAGGTCGAATGATAAGAGAAGATATCTATACAGACATAACAATCAACGTCAATTCCGAGAGAAGCATAAGAGCTCATCGAGCTGTTCTTGCTTCACAATCACCTGTGTTTCGCAGCATGTTTTCACATAATGTCAAGGAGACTGATTTGTCTACGATAAACATCACCGACATGTCAATTCAATCTTTTCAGACCTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCAGCTGACAAGTATGATATTTTTGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCTAAAAAATGTACTTGAGAGACTCCAAATTGCAACTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTATGCAATATCTTGTGAAATTTGGCAAAATATATGATATTCAGGGTGATTTAAGTTCTTTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA

Protein sequence

>MS.gene38003.t1

MNDYAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPELSKTITHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFLWEIGVPLPGKFIIDIEFLDLKTTCPKGGEACSIWPNGFMQQRQNAAALESLGRMIREDIYTDITINVNSERSIRAHRAVLASQSPVFRSMFSHNVKETDLSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIFELREACEKSLQEDIDLKNVLERLQIATLYQLTTLKFSCMQYLVKFGKIYDIQGDLSSFLQTADRDLICEVFKEILDAWKK