AlfalfaGEDB | MS.gene38003

M. sativa cultivar XinJiangDaYe / MS.gene38003

KEGG: K10523
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00651.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38003.t1	XP_003617898.1	94.5	330	16	1	1	328	1	330	1.60E-176	628.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38003.t1	Q680K8	57.0	328	139	2	1	327	1	327	7.4e-106	385.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38003.t1	G7K4T7	94.5	330	16	1	1	328	1	330	1.1e-176	628.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene38003.t1	TR	TRAF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38003.t1	MTR_5g096700	94.545	330	16	1	1	328	1	330	0.0	647
MS.gene38003.t1	MTR_5g096700	94.096	271	16	0	58	328	1	271	0.0	528
MS.gene38003.t1	MTR_3g061090	63.526	329	117	3	1	327	1	328	1.31e-150	426
MS.gene38003.t1	MTR_3g061090	64.981	257	87	3	73	327	1	256	1.94e-115	334
MS.gene38003.t1	MTR_1g029850	44.308	325	176	3	7	327	5	328	1.46e-97	291
MS.gene38003.t1	MTR_3g088560	45.963	322	169	4	5	323	2	321	1.29e-96	289
MS.gene38003.t1	MTR_5g096790	45.619	331	153	10	6	323	6	322	9.84e-89	269
MS.gene38003.t1	MTR_3g088560	46.025	239	125	3	88	323	14	251	5.01e-66	208
MS.gene38003.t1	MTR_3g088560	45.992	237	124	3	90	323	31	266	2.41e-65	207
MS.gene38003.t1	MTR_3g088560	46.465	198	102	3	129	323	3	199	8.22e-53	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38003.t1	AT1G55760	57.012	328	139	2	1	327	1	327	1.38e-136	390
MS.gene38003.t1	AT1G21780	47.516	322	166	3	7	327	5	324	4.61e-108	318
MS.gene38003.t1	AT1G21780	47.516	322	166	3	7	327	5	324	4.61e-108	318
MS.gene38003.t1	AT1G01640	29.787	188	128	4	142	327	3	188	4.33e-15	73.6
MS.gene38003.t1	AT2G39760	28.947	152	96	2	151	291	182	332	9.46e-14	71.6
MS.gene38003.t1	AT2G39760	28.947	152	96	2	151	291	182	332	1.37e-13	71.2
MS.gene38003.t1	AT2G39760	28.947	152	96	2	151	291	182	332	1.37e-13	71.2
MS.gene38003.t1	AT2G39760	28.947	152	96	2	151	291	182	332	2.40e-13	70.9
MS.gene38003.t1	AT3G56230	29.221	154	106	3	142	292	90	243	4.41e-13	68.9
MS.gene38003.t1	AT1G01640	29.586	169	115	4	161	327	1	167	4.91e-13	67.0

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Find 44 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATTGAATTGACATGT+CGG	0.667795	5.1:-77055190	None:intergenic
ACTATGAAAGATGCAATTGG+AGG	0.571379	5.1:-77054431	None:intergenic
ACTTGTCAGCTGCATGTAAT+AGG	0.350791	5.1:-77055278	None:intergenic
AGAACTTGAATCACCTGATT+GGG	0.473517	5.1:-77054492	None:intergenic
AGAATTTCTTATACATAGAT+TGG	0.343658	5.1:+77055254	MS.gene38003:CDS
AGATAAACTTCTTAGCAATA+AGG	0.369571	5.1:+77054636	MS.gene38003:CDS
AGCAAAATCTTGACCCAATC+AGG	0.352906	5.1:+77054479	MS.gene38003:CDS
AGGGTTGTGTGTGATTGTTT+TGG	0.394454	5.1:-77054411	None:intergenic
ATAGCTTTCTGTGGGAGATT+GGG	0.358877	5.1:+77054659	MS.gene38003:CDS
ATCCATTCAAAATAGCCATG+TGG	0.702367	5.1:+77054245	MS.gene38003:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATTGCATCTTTCATAGTT+AGG	+	chr5.1:77054435-77054454	MS.gene38003:CDS	30.0%
CTAACTATGAAAGATGCAAT+TGG	-	chr5.1:77054437-77054456	None:intergenic	30.0%
TCAAAGAAATTCTTGATGCT+TGG	+	chr5.1:77055523-77055542	MS.gene38003:CDS	30.0%
TTTGAAAACTACATGTCCAA+AGG	+	chr5.1:77054726-77054745	MS.gene38003:CDS	30.0%
ACTATGAAAGATGCAATTGG+AGG	-	chr5.1:77054434-77054453	None:intergenic	35.0%
AGAACTTGAATCACCTGATT+GGG	-	chr5.1:77054495-77054514	None:intergenic	35.0%
ATCCATTCAAAATAGCCATG+TGG	+	chr5.1:77054245-77054264	MS.gene38003:CDS	35.0%
CAATAAGGATAGCTTTCTGT+GGG	+	chr5.1:77054651-77054670	MS.gene38003:CDS	35.0%
CTATGAAAGATGCAATTGGA+GGG	-	chr5.1:77054433-77054452	None:intergenic	35.0%
GTTAGGATTCTTAGTTATGC+TGG	+	chr5.1:77054452-77054471	MS.gene38003:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	77054154	77055554	77054154	ID=MS.gene38003
chr5.1	mRNA	77054154	77055554	77054154	ID=MS.gene38003.t1;Parent=MS.gene38003
chr5.1	exon	77054154	77054272	77054154	ID=MS.gene38003.t1.exon1;Parent=MS.gene38003.t1
chr5.1	CDS	77054154	77054272	77054154	ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1	exon	77054355	77054500	77054355	ID=MS.gene38003.t1.exon2;Parent=MS.gene38003.t1
chr5.1	CDS	77054355	77054500	77054355	ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1	exon	77054629	77054747	77054629	ID=MS.gene38003.t1.exon3;Parent=MS.gene38003.t1
chr5.1	CDS	77054629	77054747	77054629	ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1
chr5.1	exon	77054952	77055554	77054952	ID=MS.gene38003.t1.exon4;Parent=MS.gene38003.t1
chr5.1	CDS	77054952	77055554	77054952	ID=cds.MS.gene38003.t1;Parent=MS.gene38003.t1

Gene Sequence

>MS.gene38003

ATGAATGATTATGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGTAAAGTCAAGTTTACAATTTTAATATTTTTTAGTATTATATTTGTTTACATATTGCTAAGTTTTGTTCTTATGATATGAAGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAATTGTCCAAAACAATCACACACAACCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCTGGAAATAGCAAAATCTTGACCCAATCAGGTGATTCAAGTTCTATGTTTAAAAAAGTATATAATTTAGTTAACACGAATAGTATTTGATTTAACAAGTTAGCTATAGTATTAATATGTGTGTTGATTTTGATCTTTATCTTTTCCACACTGATGTAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTCTGTGGGAGATTGGGGTTCCACTACCTGGAAAATTTATAATTGACATTGAGTTTCTTGATTTGAAAACTACATGTCCAAAGGTATGTAAGTATATAATATATCCAAATTAAGGTATTGAATAATATATGTAAACATTGATACAATTTTTTTTCCATTTACTTTTTATTTTGTTTGGTGTTGATTTTTGAAGGACTAGTCATGAAGCATAATCATAGTATTGATATGAGGGTTATTGACTTACAATTTGTTCACCTAATTTAAAATTTTCCTTGATTTGAATGAAGGGTGGGGAAGCTTGCTCTATTTGGCCTAATGGATTCATGCAGCAGAGACAAAATGCAGCGGCCCTTGAATCCCTAGGTCGAATGATAAGAGAAGATATCTATACAGACATAACAATCAACGTCAATTCCGAGAGAAGCATAAGAGCTCATCGAGCTGTTCTTGCTTCACAATCACCTGTGTTTCGCAGCATGTTTTCACATAATGTCAAGGAGACTGATTTGTCTACGATAAACATCACCGACATGTCAATTCAATCTTTTCAGACCTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCAGCTGACAAGTATGATATTTTTGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCTAAAAAATGTACTTGAGAGACTCCAAATTGCAACTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTATGCAATATCTTGTGAAATTTGGCAAAATATATGATATTCAGGGTGATTTAAGTTCTTTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA

Protein sequence

>MS.gene38003.t1

MNDYAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPELSKTITHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFLWEIGVPLPGKFIIDIEFLDLKTTCPKGGEACSIWPNGFMQQRQNAAALESLGRMIREDIYTDITINVNSERSIRAHRAVLASQSPVFRSMFSHNVKETDLSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIFELREACEKSLQEDIDLKNVLERLQIATLYQLTTLKFSCMQYLVKFGKIYDIQGDLSSFLQTADRDLICEVFKEILDAWKK