AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38062

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38062.t1 MTR_5g097480 98.897 544 6 0 2 545 1 544 0.0 1129
MS.gene38062.t1 MTR_3g056110 57.532 551 218 5 2 545 1 542 0.0 647
MS.gene38062.t1 MTR_5g094450 56.937 555 205 8 2 545 1 532 0.0 619
MS.gene38062.t1 MTR_4g133660 57.350 551 218 8 2 544 1 542 0.0 608
MS.gene38062.t1 MTR_2g082090 63.824 387 137 1 158 544 195 578 0.0 525
MS.gene38062.t1 MTR_2g026250 46.949 590 248 8 13 545 15 596 1.87e-178 516
MS.gene38062.t1 MTR_1g029420 56.216 370 162 0 176 545 223 592 2.93e-159 467
MS.gene38062.t1 MTR_3g089055 54.278 374 171 0 172 545 192 565 5.01e-151 445
MS.gene38062.t1 MTR_3g089055 54.278 374 171 0 172 545 197 570 6.91e-151 444
MS.gene38062.t1 MTR_7g027190 35.603 514 271 9 50 545 202 673 3.32e-87 283
MS.gene38062.t1 MTR_3g065980 36.923 390 209 7 175 545 169 540 2.73e-73 243
MS.gene38062.t1 MTR_7g062120 34.048 373 245 1 174 545 368 740 3.30e-69 237
MS.gene38062.t1 MTR_4g064160 32.796 372 249 1 174 544 312 683 2.97e-65 225
MS.gene38062.t1 MTR_8g442410 33.594 384 234 7 174 545 151 525 7.80e-65 220
MS.gene38062.t1 MTR_4g064150 32.447 376 249 3 174 544 357 732 1.15e-64 224
MS.gene38062.t1 MTR_4g064200 33.245 376 246 4 171 544 277 649 4.68e-64 221
MS.gene38062.t1 MTR_2g097390 29.150 494 310 10 75 544 163 640 1.08e-63 220
MS.gene38062.t1 MTR_7g057230 33.494 415 255 7 141 544 252 656 4.01e-63 219
MS.gene38062.t1 MTR_2g097310 28.279 488 317 9 75 544 165 637 7.68e-62 215
MS.gene38062.t1 MTR_3g022830 31.265 419 228 7 179 545 26 436 1.11e-60 207
MS.gene38062.t1 MTR_6g047750 35.354 396 204 13 176 545 293 662 1.55e-60 212
MS.gene38062.t1 MTR_4g076140 30.787 432 231 7 175 545 45 469 3.22e-60 206
MS.gene38062.t1 MTR_2g097350 31.200 375 249 4 174 544 270 639 6.70e-60 209
MS.gene38062.t1 MTR_4g064120 29.904 418 282 4 130 542 212 623 1.26e-59 208
MS.gene38062.t1 MTR_2g097410 31.117 376 252 4 174 544 367 740 1.47e-59 210
MS.gene38062.t1 MTR_4g104020 32.292 384 238 6 175 545 146 520 1.59e-59 206
MS.gene38062.t1 MTR_2g097467 31.095 402 270 4 144 544 258 653 3.61e-59 208
MS.gene38062.t1 MTR_4g064180 31.903 373 249 3 174 541 250 622 4.15e-59 207
MS.gene38062.t1 MTR_4g097080 30.890 382 243 7 176 545 131 503 4.38e-59 204
MS.gene38062.t1 MTR_5g009080 29.792 433 235 7 175 545 53 478 6.10e-58 201
MS.gene38062.t1 MTR_2g097473 32.976 373 244 5 174 544 285 653 6.38e-58 204
MS.gene38062.t1 MTR_7g074650 34.511 368 220 7 182 545 442 792 9.65e-58 206
MS.gene38062.t1 MTR_2g097463 31.720 372 250 3 174 544 286 654 7.60e-56 199
MS.gene38062.t1 MTR_4g102790 32.461 382 237 10 175 544 289 661 8.78e-52 188
MS.gene38062.t1 MTR_5g015490 29.592 392 253 8 170 545 106 490 9.47e-52 184
MS.gene38062.t1 MTR_1g096030 31.778 450 254 16 128 545 31 459 2.04e-49 177
MS.gene38062.t1 MTR_2g097380 27.197 478 296 11 75 544 127 560 2.15e-49 179
MS.gene38062.t1 MTR_4g077760 32.979 376 237 8 175 545 190 555 5.13e-47 172
MS.gene38062.t1 MTR_4g076020 33.062 369 215 9 182 545 88 429 2.10e-46 168
MS.gene38062.t1 MTR_5g058860 31.008 387 244 10 175 544 116 496 6.51e-44 163
MS.gene38062.t1 MTR_3g072710 29.426 401 250 10 168 544 108 499 5.36e-43 160
MS.gene38062.t1 MTR_2g089100 27.295 403 251 8 175 544 49 442 1.19e-41 155
MS.gene38062.t1 MTR_2g099110 28.974 390 243 9 178 545 74 451 4.98e-40 151
MS.gene38062.t1 MTR_1g069725 30.053 376 240 10 174 544 105 462 6.45e-40 151
MS.gene38062.t1 MTR_2g034250 25.974 385 257 10 179 545 211 585 1.91e-35 140
MS.gene38062.t1 MTR_4g095500 26.794 418 252 9 175 544 56 467 2.62e-34 135
MS.gene38062.t1 MTR_5g015950 25.806 434 238 12 175 544 32 445 3.34e-34 135
MS.gene38062.t1 MTR_3g053270 25.806 434 238 12 175 544 32 445 3.34e-34 135
MS.gene38062.t1 MTR_2g034260 25.882 340 229 8 179 504 211 541 1.97e-30 125
MS.gene38062.t1 MTR_2g034280 24.084 382 273 7 175 545 200 575 2.37e-30 125
MS.gene38062.t1 MTR_4g026485 24.595 370 261 8 179 545 270 624 2.85e-29 122
MS.gene38062.t1 MTR_0092s0100 23.662 355 254 7 194 545 391 731 3.32e-26 113
MS.gene38062.t1 MTR_0092s0100 23.662 355 254 7 194 545 388 728 3.52e-26 113
MS.gene38062.t1 MTR_7g109580 23.770 366 248 10 191 545 209 554 6.70e-26 112
MS.gene38062.t1 MTR_8g093070 28.186 408 249 19 155 544 114 495 9.75e-26 111
MS.gene38062.t1 MTR_1g086970 23.143 350 246 10 209 545 139 478 1.98e-20 94.7
MS.gene38062.t1 MTR_8g020840 25.185 405 243 16 176 545 169 548 5.27e-19 90.9
MS.gene38062.t1 MTR_3g022580 39.286 112 64 2 436 545 74 183 3.52e-18 83.2
MS.gene38062.t1 MTR_3g022005 43.158 95 50 2 453 545 33 125 1.53e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38062.t1 AT5G48150 65.492 539 131 7 13 545 1 490 0.0 700
MS.gene38062.t1 AT5G48150 65.492 539 131 7 13 545 1 490 0.0 700
MS.gene38062.t1 AT5G48150 65.492 539 131 7 13 545 1 490 0.0 700
MS.gene38062.t1 AT5G48150 65.492 539 131 7 13 545 1 490 0.0 700
MS.gene38062.t1 AT1G50600 61.943 494 180 4 57 545 107 597 0.0 618
MS.gene38062.t1 AT1G50600 61.943 494 180 4 57 545 111 601 0.0 618
MS.gene38062.t1 AT1G50600 61.943 494 180 4 57 545 36 526 0.0 616
MS.gene38062.t1 AT2G04890 68.599 414 123 1 132 545 7 413 0.0 595
MS.gene38062.t1 AT4G17230 53.016 547 232 9 2 545 1 525 0.0 559
MS.gene38062.t1 AT4G17230 53.016 547 232 9 2 545 1 525 0.0 559
MS.gene38062.t1 AT1G21450 48.649 444 211 4 119 545 150 593 1.36e-150 445
MS.gene38062.t1 AT3G03450 36.800 375 224 7 175 545 180 545 1.25e-70 236
MS.gene38062.t1 AT1G66350 35.678 398 221 11 159 545 133 506 1.47e-70 235
MS.gene38062.t1 AT1G14920 36.061 391 225 9 164 545 155 529 3.96e-70 234
MS.gene38062.t1 AT2G01570 36.869 396 221 12 161 545 204 581 1.76e-68 231
MS.gene38062.t1 AT1G07530 33.333 375 248 2 172 544 390 764 2.70e-68 235
MS.gene38062.t1 AT5G52510 34.286 385 228 7 176 545 266 640 2.42e-66 227
MS.gene38062.t1 AT5G17490 34.933 375 225 9 175 545 157 516 1.93e-65 222
MS.gene38062.t1 AT2G29060 32.533 375 249 3 174 544 280 654 1.22e-64 223
MS.gene38062.t1 AT5G59450 31.383 376 254 3 174 545 223 598 1.34e-64 221
MS.gene38062.t1 AT2G29060 32.267 375 250 2 174 544 317 691 1.85e-64 223
MS.gene38062.t1 AT2G37650 31.903 373 251 2 174 545 331 701 3.23e-61 214
MS.gene38062.t1 AT3G46600 31.452 372 253 2 175 545 1 371 4.41e-61 206
MS.gene38062.t1 AT2G37650 31.903 373 251 2 174 545 343 713 4.80e-61 214
MS.gene38062.t1 AT1G50420 29.792 433 235 7 175 545 54 479 1.13e-60 208
MS.gene38062.t1 AT3G46600 31.367 373 254 2 174 545 208 579 4.59e-60 209
MS.gene38062.t1 AT3G46600 31.367 373 254 2 174 545 176 547 6.41e-60 207
MS.gene38062.t1 AT3G54220 34.605 367 222 7 182 544 297 649 2.70e-56 200
MS.gene38062.t1 AT2G29065 32.461 382 245 7 174 544 248 627 3.44e-56 199
MS.gene38062.t1 AT1G07520 29.557 406 274 5 149 544 125 528 3.59e-55 194
MS.gene38062.t1 AT1G07520 29.557 406 274 5 149 544 125 528 3.59e-55 194
MS.gene38062.t1 AT1G07520 29.557 406 274 5 149 544 289 692 1.13e-54 196
MS.gene38062.t1 AT1G07520 30.446 381 253 5 174 544 315 693 1.27e-54 196
MS.gene38062.t1 AT5G66770 34.595 370 227 8 182 545 224 584 1.19e-51 186
MS.gene38062.t1 AT5G41920 32.718 379 231 9 174 545 39 400 2.49e-48 173
MS.gene38062.t1 AT3G50650 35.294 357 207 9 201 545 198 542 8.69e-48 174
MS.gene38062.t1 AT4G37650 29.104 402 247 12 171 545 139 529 1.35e-47 174
MS.gene38062.t1 AT1G55580 30.994 342 204 8 227 545 113 445 1.22e-44 164
MS.gene38062.t1 AT1G63100 32.665 349 217 6 175 516 274 611 6.03e-44 166
MS.gene38062.t1 AT1G63100 32.665 349 217 6 175 516 249 586 7.08e-44 165
MS.gene38062.t1 AT4G08250 28.079 406 270 9 148 544 84 476 2.20e-39 150
MS.gene38062.t1 AT3G49950 25.389 386 267 9 175 544 27 407 3.28e-31 125
MS.gene38062.t1 AT2G45160 25.532 376 231 13 192 545 291 639 5.73e-27 115
MS.gene38062.t1 AT3G60630 28.571 301 190 8 257 545 335 622 8.40e-25 108
MS.gene38062.t1 AT3G13840 27.749 382 247 12 176 545 146 510 3.28e-24 106
MS.gene38062.t1 AT4G36710 25.000 380 252 12 179 545 126 485 1.25e-20 95.5
MS.gene38062.t1 AT4G00150 23.098 368 253 11 183 545 212 554 2.76e-20 94.7
MS.gene38062.t1 AT5G67411 33.929 112 72 2 371 482 89 198 1.15e-11 64.7

Find 123 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCAGATGGTGTCGGTTTC+TGG 0.116927 5.1:+77819457 MS.gene38062:CDS
GTTTGCCCTTACTTCAAATT+TGG 0.195361 5.1:+77819625 MS.gene38062:CDS
ATTGGCTTCCTTGAGCAATT+TGG 0.205067 5.1:-77819717 None:intergenic
CAACAAGTGTCACGACTTTC+GGG 0.226734 5.1:-77820074 None:intergenic
CATTTCAAGCGAAGCGAAAT+TGG 0.230694 5.1:-77819311 None:intergenic
CCAATGGATTTCGTTGATTC+AGG 0.238320 5.1:+77819735 MS.gene38062:CDS
TCAACAAGTGTCACGACTTT+CGG 0.277772 5.1:-77820075 None:intergenic
TCCTTACCCTCTGAGTTCTT+TGG 0.281649 5.1:+77820344 MS.gene38062:CDS
CAATTTCGCTTCGCTTGAAA+TGG 0.314726 5.1:+77819312 MS.gene38062:CDS
TCGATATTGCGGCAAGCAAT+AGG 0.317589 5.1:-77818921 None:intergenic
CAAGCACAAGCTTAGAGAAT+TGG 0.336532 5.1:+77819222 MS.gene38062:CDS
CAATGTTGAGCAGCATTGTT+TGG 0.339609 5.1:+77820218 MS.gene38062:CDS
CCACCACACATCCGGATTAC+AGG 0.342966 5.1:+77819781 MS.gene38062:CDS
TAGCATGAATGCGAAATTTA+CGG 0.352242 5.1:-77819977 None:intergenic
GTGTCAGCACTCAGAATCAT+AGG 0.362236 5.1:+77820019 MS.gene38062:CDS
ATCATTGTCTGCAATTGCTT+TGG 0.368256 5.1:-77819401 None:intergenic
AATGGTTGATGGACGAATTA+CGG 0.368703 5.1:+77819437 MS.gene38062:CDS
TGGTGAACCGATTCAAAGAT+TGG 0.393469 5.1:+77819477 MS.gene38062:CDS
CATAACCTTGCGGTTCGGCC+AGG 0.394232 5.1:+77819943 MS.gene38062:CDS
CTTGAAGCTACTTAAATCTT+CGG 0.395656 5.1:-77819203 None:intergenic
CCGGATGTGTGGTGGTCCCC+CGG 0.395807 5.1:-77819773 None:intergenic
CCTCTGAGTTCTTTGGTGAA+TGG 0.405916 5.1:+77820351 MS.gene38062:CDS
CGATATTGCGGCAAGCAATA+GGG 0.411999 5.1:-77818920 None:intergenic
GTTGCACGGTTGTCTGCATC+CGG 0.415709 5.1:+77819523 MS.gene38062:CDS
TGCAGACAATGATTTGCTAA+TGG 0.419025 5.1:+77819411 MS.gene38062:CDS
ATTTGGTTGCTTCTTGTGCT+TGG 0.429491 5.1:+77820463 MS.gene38062:CDS
ATGTAACCAAATTTGAAGTA+AGG 0.431720 5.1:-77819631 None:intergenic
TTTGGTTACATGTCTGCAAA+TGG 0.433675 5.1:+77819643 MS.gene38062:CDS
GTTATGCACGATCCACTCAT+TGG 0.435852 5.1:-77819181 None:intergenic
GCTAATGGCGCAATGGTTGA+TGG 0.437770 5.1:+77819426 MS.gene38062:CDS
TGAGATTCTTGCTGCGACAT+TGG 0.437774 5.1:-77819109 None:intergenic
TTTCACGCTGCAGCTATCTC+TGG 0.438144 5.1:+77819904 MS.gene38062:CDS
AAAGCTTGCTCGGCATTTCA+AGG 0.448893 5.1:+77819870 MS.gene38062:CDS
AGATTTGTAGATTGAACTCC+CGG 0.451937 5.1:-77819542 None:intergenic
GGCGAGCCATAGGTATTGTC+GGG 0.452043 5.1:-77819160 None:intergenic
ACTCCAACGTGCAGTAATAA+AGG 0.452114 5.1:-77819015 None:intergenic
TTGGAGTCGTCCTTGGCAGC+TGG 0.456230 5.1:+77819031 MS.gene38062:CDS
TGGTTGGATGAATAGAGATT+TGG 0.456807 5.1:+77820446 MS.gene38062:CDS
AACTGCATAACCTTGCGGTT+CGG 0.459814 5.1:+77819938 MS.gene38062:CDS
TCAGCTTATGCCCGTGGAGG+TGG 0.460311 5.1:+77819820 MS.gene38062:CDS
ATCCTCTCTTTATGCTCTCT+CGG 0.463692 5.1:-77820195 None:intergenic
AGGTCAAGATTTGCAATGGC+TGG 0.465717 5.1:+77820315 MS.gene38062:CDS
TGGCGAGCCATAGGTATTGT+CGG 0.470434 5.1:-77819161 None:intergenic
GAGAGAAGCTCGGCACTCTC+TGG 0.471288 5.1:-77819580 None:intergenic
TAGGGTTCAATTTGCTGCAA+TGG 0.476650 5.1:-77818902 None:intergenic
TATGCTCCCAATCTTTGAAT+CGG 0.477205 5.1:-77819484 None:intergenic
AGAGATGGAGCTCTATATCT+TGG 0.480538 5.1:+77820426 MS.gene38062:CDS
TGAGTGCTGACACTCTCATC+TGG 0.480842 5.1:-77820009 None:intergenic
AGGCCAAGCAACTCAATTCT+CGG 0.481276 5.1:+77818976 MS.gene38062:CDS
GGTTCGGCCAGGGGAAGCTC+TGG 0.484150 5.1:+77819954 MS.gene38062:CDS
GGTGAACCGATTCAAAGATT+GGG 0.489961 5.1:+77819478 MS.gene38062:CDS
CTGCACGTTGGAGTCGTCCT+TGG 0.491134 5.1:+77819024 MS.gene38062:CDS
CTATCAAGATTATCAGAGTC+AGG 0.491387 5.1:-77819262 None:intergenic
ATAACCTTGCGGTTCGGCCA+GGG 0.491957 5.1:+77819944 MS.gene38062:CDS
GAAATCGATTATATGAACTC+TGG 0.496688 5.1:-77819695 None:intergenic
GAGCCGAGAATTGAGTTGCT+TGG 0.498447 5.1:-77818979 None:intergenic
TAATAAAGGTCAGAAGAAGT+TGG 0.499526 5.1:-77819001 None:intergenic
CATCAGCTGTACAACAATGA+TGG 0.501721 5.1:+77818953 MS.gene38062:CDS
AACAAAATTTCCAGCTGCCA+AGG 0.505944 5.1:-77819041 None:intergenic
CTTCGCTTGAAATGGACAGT+TGG 0.507924 5.1:+77819320 MS.gene38062:CDS
ACCAAAGAACTCAGAGGGTA+AGG 0.509605 5.1:-77820345 None:intergenic
GATCATCAATACCTGTAATC+CGG 0.510867 5.1:-77819792 None:intergenic
GACACGAGCTGCTAGGGAAG+TGG 0.514693 5.1:+77820292 MS.gene38062:CDS
GTGTCACGACTTTCGGGGAT+AGG 0.518113 5.1:-77820068 None:intergenic
TCCACTCATTGGCGAGCCAT+AGG 0.519637 5.1:-77819170 None:intergenic
TGGAACGACACGAGCTGCTA+GGG 0.521228 5.1:+77820286 MS.gene38062:CDS
GTGGAACGACACGAGCTGCT+AGG 0.524981 5.1:+77820285 MS.gene38062:CDS
ATGGAGCTCTATATCTTGGT+TGG 0.526117 5.1:+77820430 MS.gene38062:CDS
TTGCTTTGGCACATGCAGTA+AGG 0.529396 5.1:-77819387 None:intergenic
GTTAACATCATAGCTTGTGA+AGG 0.534917 5.1:+77820252 MS.gene38062:CDS
CGAATTACGGCAGATGGTGT+CGG 0.542149 5.1:+77819450 MS.gene38062:CDS
TGATTTAGATTTCGATATTG+CGG 0.543411 5.1:-77818932 None:intergenic
GCTGCAGCGTGAAACTCAAA+TGG 0.545283 5.1:-77819895 None:intergenic
TGTAACCAAATTTGAAGTAA+GGG 0.545514 5.1:-77819630 None:intergenic
TAACCTTGCGGTTCGGCCAG+GGG 0.545938 5.1:+77819945 MS.gene38062:CDS
ACCTATGGCTCGCCAATGAG+TGG 0.546193 5.1:+77819169 MS.gene38062:CDS
ATCGATTTCCAAATTGCTCA+AGG 0.546444 5.1:+77819709 MS.gene38062:CDS
GCACTCAGAATCATAGGGAT+AGG 0.551891 5.1:+77820025 MS.gene38062:CDS
TTAACATCATAGCTTGTGAA+GGG 0.552742 5.1:+77820253 MS.gene38062:CDS
CCTGAATCAACGAAATCCAT+TGG 0.554910 5.1:-77819735 None:intergenic
GGGGAATGATACTTATCAGA+TGG 0.562828 5.1:-77819139 None:intergenic
TGCGACATTGGACTATCGTT+AGG 0.563587 5.1:-77819097 None:intergenic
TTTACGGCCAGAGCTTCCCC+TGG 0.565827 5.1:-77819961 None:intergenic
GAATTGGAAAATGTAATGCT+TGG 0.570289 5.1:+77819238 MS.gene38062:CDS
CTTCCCCTGGCCGAACCGCA+AGG 0.580073 5.1:-77819948 None:intergenic
TTGCACGGTTGTCTGCATCC+GGG 0.585418 5.1:+77819524 MS.gene38062:CDS
TGTTGGAAGGACTCGTTGCA+CGG 0.585796 5.1:+77819509 MS.gene38062:CDS
TATATGTAGTCCACCTCCAC+GGG 0.589655 5.1:-77819830 None:intergenic
TATAGACTTGAAGAGAGAGA+TGG 0.590094 5.1:+77820411 MS.gene38062:CDS
TTGTGAAGGGGTCGAGAGAG+TGG 0.594827 5.1:+77820266 MS.gene38062:CDS
GACTACATATAGTTGAAAAG+AGG 0.595404 5.1:+77819842 MS.gene38062:CDS
TGTCAGCACTCAGAATCATA+GGG 0.595636 5.1:+77820020 MS.gene38062:CDS
AGAGGTTATCAAAGCTTGCT+CGG 0.596778 5.1:+77819860 MS.gene38062:CDS
AAGATTGGGAGCATACATGT+TGG 0.605912 5.1:+77819492 MS.gene38062:CDS
CTATATGTAGTCCACCTCCA+CGG 0.608482 5.1:-77819831 None:intergenic
TCCATAGTTTCGAGAAAACG+TGG 0.611195 5.1:-77820132 None:intergenic
GTGGAGGTCAAGATTTGCAA+TGG 0.615832 5.1:+77820311 MS.gene38062:CDS
TTGGGAGCATACATGTTGGA+AGG 0.617711 5.1:+77819496 MS.gene38062:CDS
TACGACAGTGCTATCAGCAA+CGG 0.617830 5.1:+77819286 MS.gene38062:CDS
TGACCTTTATTACTGCACGT+TGG 0.620270 5.1:+77819012 MS.gene38062:CDS
GGCGAGAAACTAACAGTCGA+AGG 0.623572 5.1:-77819076 None:intergenic
AAATTGCTCAAGGAAGCCAA+TGG 0.626215 5.1:+77819719 MS.gene38062:CDS
GATGGACGAATTACGGCAGA+TGG 0.627072 5.1:+77819444 MS.gene38062:CDS
TTACATGAACATACTTTACG+AGG 0.627108 5.1:+77819603 MS.gene38062:CDS
ACGAGCTGCTAGGGAAGTGG+AGG 0.634312 5.1:+77820295 MS.gene38062:CDS
CCACGGGCATAAGCTGATGT+CGG 0.634703 5.1:-77819814 None:intergenic
CATTCACCAAAGAACTCAGA+GGG 0.637813 5.1:-77820350 None:intergenic
CCGACATCAGCTTATGCCCG+TGG 0.638695 5.1:+77819814 MS.gene38062:CDS
ATGATTTGCTAATGGCGCAA+TGG 0.646542 5.1:+77819419 MS.gene38062:CDS
TTCCGAGAGAGCATAAAGAG+AGG 0.651166 5.1:+77820193 MS.gene38062:CDS
GCGAGCCATAGGTATTGTCG+GGG 0.657718 5.1:-77819159 None:intergenic
CAGCTGTACAACAATGATGG+AGG 0.659045 5.1:+77818956 MS.gene38062:CDS
TGTTCAACTGCATAACCTTG+CGG 0.660074 5.1:+77819933 MS.gene38062:CDS
ACATCAGCTTATGCCCGTGG+AGG 0.663564 5.1:+77819817 MS.gene38062:CDS
TAACATCATAGCTTGTGAAG+GGG 0.668817 5.1:+77820254 MS.gene38062:CDS
CCTGTAATCCGGATGTGTGG+TGG 0.675147 5.1:-77819781 None:intergenic
GGACAGTTGGAGACAAACAA+TGG 0.681512 5.1:+77819333 MS.gene38062:CDS
AACAAGTGTCACGACTTTCG+GGG 0.682943 5.1:-77820073 None:intergenic
GTTCATGTAAGAGAGAAGCT+CGG 0.686963 5.1:-77819590 None:intergenic
CCATTCACCAAAGAACTCAG+AGG 0.710837 5.1:-77820351 None:intergenic
ATACCTGTAATCCGGATGTG+TGG 0.719840 5.1:-77819784 None:intergenic
CGAGCCATAGGTATTGTCGG+GGG 0.727707 5.1:-77819158 None:intergenic
AGAATCTAACACAAACACTG+CGG 0.784454 5.1:+77820101 MS.gene38062:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTTAGATTTCGATATTG+CGG - chr5.1:77818935-77818954 None:intergenic 25.0%
!! ATGTAACCAAATTTGAAGTA+AGG - chr5.1:77819634-77819653 None:intergenic 25.0%
!! TGTAACCAAATTTGAAGTAA+GGG - chr5.1:77819633-77819652 None:intergenic 25.0%
!!! GTTTTCAAGCAATTTCTTTA+TGG - chr5.1:77820379-77820398 None:intergenic 25.0%
CTTGAAGCTACTTAAATCTT+CGG - chr5.1:77819206-77819225 None:intergenic 30.0%
GAAATCGATTATATGAACTC+TGG - chr5.1:77819698-77819717 None:intergenic 30.0%
GAATTGGAAAATGTAATGCT+TGG + chr5.1:77819238-77819257 MS.gene38062:CDS 30.0%
GACTACATATAGTTGAAAAG+AGG + chr5.1:77819842-77819861 MS.gene38062:CDS 30.0%
TAATAAAGGTCAGAAGAAGT+TGG - chr5.1:77819004-77819023 None:intergenic 30.0%
TAGCATGAATGCGAAATTTA+CGG - chr5.1:77819980-77819999 None:intergenic 30.0%
TTACATGAACATACTTTACG+AGG + chr5.1:77819603-77819622 MS.gene38062:CDS 30.0%
! TTAACATCATAGCTTGTGAA+GGG + chr5.1:77820253-77820272 MS.gene38062:CDS 30.0%
AGAATCTAACACAAACACTG+CGG + chr5.1:77820101-77820120 MS.gene38062:CDS 35.0%
AGATTTGTAGATTGAACTCC+CGG - chr5.1:77819545-77819564 None:intergenic 35.0%
ATCATTGTCTGCAATTGCTT+TGG - chr5.1:77819404-77819423 None:intergenic 35.0%
CTATCAAGATTATCAGAGTC+AGG - chr5.1:77819265-77819284 None:intergenic 35.0%
GATCATCAATACCTGTAATC+CGG - chr5.1:77819795-77819814 None:intergenic 35.0%
GTTTGCCCTTACTTCAAATT+TGG + chr5.1:77819625-77819644 MS.gene38062:CDS 35.0%
TATAGACTTGAAGAGAGAGA+TGG + chr5.1:77820411-77820430 MS.gene38062:CDS 35.0%
TATGCTCCCAATCTTTGAAT+CGG - chr5.1:77819487-77819506 None:intergenic 35.0%
TGGTTGGATGAATAGAGATT+TGG + chr5.1:77820446-77820465 MS.gene38062:CDS 35.0%
TTTGGTTACATGTCTGCAAA+TGG + chr5.1:77819643-77819662 MS.gene38062:CDS 35.0%
! ATCATAGGGATAGGCTTTTA+AGG + chr5.1:77820034-77820053 MS.gene38062:CDS 35.0%
! ATCGATTTCCAAATTGCTCA+AGG + chr5.1:77819709-77819728 MS.gene38062:CDS 35.0%
! ATTTTTCATGGCTTCTGCAA+TGG - chr5.1:77819671-77819690 None:intergenic 35.0%
! GTTAACATCATAGCTTGTGA+AGG + chr5.1:77820252-77820271 MS.gene38062:CDS 35.0%
! TAACATCATAGCTTGTGAAG+GGG + chr5.1:77820254-77820273 MS.gene38062:CDS 35.0%
!! AACTCTGGCTTCATTTTTCA+TGG - chr5.1:77819683-77819702 None:intergenic 35.0%
!! AATGGTTGATGGACGAATTA+CGG + chr5.1:77819437-77819456 MS.gene38062:CDS 35.0%
!! TGCAGACAATGATTTGCTAA+TGG + chr5.1:77819411-77819430 MS.gene38062:CDS 35.0%
AAATTGCTCAAGGAAGCCAA+TGG + chr5.1:77819719-77819738 MS.gene38062:CDS 40.0%
AACAAAATTTCCAGCTGCCA+AGG - chr5.1:77819044-77819063 None:intergenic 40.0%
ACTCCAACGTGCAGTAATAA+AGG - chr5.1:77819018-77819037 None:intergenic 40.0%
AGAGATGGAGCTCTATATCT+TGG + chr5.1:77820426-77820445 MS.gene38062:CDS 40.0%
ATCCTCTCTTTATGCTCTCT+CGG - chr5.1:77820198-77820217 None:intergenic 40.0%
ATGGAGCTCTATATCTTGGT+TGG + chr5.1:77820430-77820449 MS.gene38062:CDS 40.0%
ATTTGGTTGCTTCTTGTGCT+TGG + chr5.1:77820463-77820482 MS.gene38062:CDS 40.0%
CAAGCACAAGCTTAGAGAAT+TGG + chr5.1:77819222-77819241 MS.gene38062:CDS 40.0%
CAATGTTGAGCAGCATTGTT+TGG + chr5.1:77820218-77820237 MS.gene38062:CDS 40.0%
CAATTTCGCTTCGCTTGAAA+TGG + chr5.1:77819312-77819331 MS.gene38062:CDS 40.0%
CATCAGCTGTACAACAATGA+TGG + chr5.1:77818953-77818972 MS.gene38062:CDS 40.0%
CATTCACCAAAGAACTCAGA+GGG - chr5.1:77820353-77820372 None:intergenic 40.0%
CATTTCAAGCGAAGCGAAAT+TGG - chr5.1:77819314-77819333 None:intergenic 40.0%
CCTGAATCAACGAAATCCAT+TGG - chr5.1:77819738-77819757 None:intergenic 40.0%
GGGGAATGATACTTATCAGA+TGG - chr5.1:77819142-77819161 None:intergenic 40.0%
GTTCATGTAAGAGAGAAGCT+CGG - chr5.1:77819593-77819612 None:intergenic 40.0%
TCAACAAGTGTCACGACTTT+CGG - chr5.1:77820078-77820097 None:intergenic 40.0%
TCCATAGTTTCGAGAAAACG+TGG - chr5.1:77820135-77820154 None:intergenic 40.0%
TGACCTTTATTACTGCACGT+TGG + chr5.1:77819012-77819031 MS.gene38062:CDS 40.0%
TGTCAGCACTCAGAATCATA+GGG + chr5.1:77820020-77820039 MS.gene38062:CDS 40.0%
TGTTCAACTGCATAACCTTG+CGG + chr5.1:77819933-77819952 MS.gene38062:CDS 40.0%
! AAGATTGGGAGCATACATGT+TGG + chr5.1:77819492-77819511 MS.gene38062:CDS 40.0%
! AGAGGTTATCAAAGCTTGCT+CGG + chr5.1:77819860-77819879 MS.gene38062:CDS 40.0%
! ATTGGCTTCCTTGAGCAATT+TGG - chr5.1:77819720-77819739 None:intergenic 40.0%
! GGTGAACCGATTCAAAGATT+GGG + chr5.1:77819478-77819497 MS.gene38062:CDS 40.0%
! TCCACGTTTTCTCGAAACTA+TGG + chr5.1:77820131-77820150 MS.gene38062:CDS 40.0%
! TGGTGAACCGATTCAAAGAT+TGG + chr5.1:77819477-77819496 MS.gene38062:CDS 40.0%
!! ATGATTTGCTAATGGCGCAA+TGG + chr5.1:77819419-77819438 MS.gene38062:CDS 40.0%
!! CCAATGGATTTCGTTGATTC+AGG + chr5.1:77819735-77819754 MS.gene38062:CDS 40.0%
!! TAGGGTTCAATTTGCTGCAA+TGG - chr5.1:77818905-77818924 None:intergenic 40.0%
!!! TAGGGATAGGCTTTTAAGGT+TGG + chr5.1:77820038-77820057 MS.gene38062:CDS 40.0%
AACAAGTGTCACGACTTTCG+GGG - chr5.1:77820076-77820095 None:intergenic 45.0%
AACTGCATAACCTTGCGGTT+CGG + chr5.1:77819938-77819957 MS.gene38062:CDS 45.0%
ACCAAAGAACTCAGAGGGTA+AGG - chr5.1:77820348-77820367 None:intergenic 45.0%
AGGCCAAGCAACTCAATTCT+CGG + chr5.1:77818976-77818995 MS.gene38062:CDS 45.0%
AGGTCAAGATTTGCAATGGC+TGG + chr5.1:77820315-77820334 MS.gene38062:CDS 45.0%
ATACCTGTAATCCGGATGTG+TGG - chr5.1:77819787-77819806 None:intergenic 45.0%
CAACAAGTGTCACGACTTTC+GGG - chr5.1:77820077-77820096 None:intergenic 45.0%
CAGCTGTACAACAATGATGG+AGG + chr5.1:77818956-77818975 MS.gene38062:CDS 45.0%
CCATTCACCAAAGAACTCAG+AGG - chr5.1:77820354-77820373 None:intergenic 45.0%
CCTCTGAGTTCTTTGGTGAA+TGG + chr5.1:77820351-77820370 MS.gene38062:CDS 45.0%
CGATATTGCGGCAAGCAATA+GGG - chr5.1:77818923-77818942 None:intergenic 45.0%
CTATATGTAGTCCACCTCCA+CGG - chr5.1:77819834-77819853 None:intergenic 45.0%
CTTCGCTTGAAATGGACAGT+TGG + chr5.1:77819320-77819339 MS.gene38062:CDS 45.0%
GCACTCAGAATCATAGGGAT+AGG + chr5.1:77820025-77820044 MS.gene38062:CDS 45.0%
GGACAGTTGGAGACAAACAA+TGG + chr5.1:77819333-77819352 MS.gene38062:CDS 45.0%
GTGGAGGTCAAGATTTGCAA+TGG + chr5.1:77820311-77820330 MS.gene38062:CDS 45.0%
GTGTCAGCACTCAGAATCAT+AGG + chr5.1:77820019-77820038 MS.gene38062:CDS 45.0%
GTTATGCACGATCCACTCAT+TGG - chr5.1:77819184-77819203 None:intergenic 45.0%
TACGACAGTGCTATCAGCAA+CGG + chr5.1:77819286-77819305 MS.gene38062:CDS 45.0%
TATATGTAGTCCACCTCCAC+GGG - chr5.1:77819833-77819852 None:intergenic 45.0%
TCCTTACCCTCTGAGTTCTT+TGG + chr5.1:77820344-77820363 MS.gene38062:CDS 45.0%
TCGATATTGCGGCAAGCAAT+AGG - chr5.1:77818924-77818943 None:intergenic 45.0%
TGAGATTCTTGCTGCGACAT+TGG - chr5.1:77819112-77819131 None:intergenic 45.0%
TGCGACATTGGACTATCGTT+AGG - chr5.1:77819100-77819119 None:intergenic 45.0%
TTGCTTTGGCACATGCAGTA+AGG - chr5.1:77819390-77819409 None:intergenic 45.0%
! AAAGCTTGCTCGGCATTTCA+AGG + chr5.1:77819870-77819889 MS.gene38062:CDS 45.0%
! TGATTCAGGCTTTTGCAGCT+AGG + chr5.1:77819749-77819768 MS.gene38062:CDS 45.0%
! TTCCGAGAGAGCATAAAGAG+AGG + chr5.1:77820193-77820212 MS.gene38062:CDS 45.0%
! TTGGGAGCATACATGTTGGA+AGG + chr5.1:77819496-77819515 MS.gene38062:CDS 45.0%
CATTCCCCCGACAATACCTA+TGG + chr5.1:77819154-77819173 MS.gene38062:CDS 50.0%
CGAATTACGGCAGATGGTGT+CGG + chr5.1:77819450-77819469 MS.gene38062:CDS 50.0%
GATGGACGAATTACGGCAGA+TGG + chr5.1:77819444-77819463 MS.gene38062:CDS 50.0%
GCTAATGGCGCAATGGTTGA+TGG + chr5.1:77819426-77819445 MS.gene38062:CDS 50.0%
GCTGCAGCGTGAAACTCAAA+TGG - chr5.1:77819898-77819917 None:intergenic 50.0%
GGCGAGAAACTAACAGTCGA+AGG - chr5.1:77819079-77819098 None:intergenic 50.0%
TGTTGGAAGGACTCGTTGCA+CGG + chr5.1:77819509-77819528 MS.gene38062:CDS 50.0%
TTTCACGCTGCAGCTATCTC+TGG + chr5.1:77819904-77819923 MS.gene38062:CDS 50.0%
! GAGCCGAGAATTGAGTTGCT+TGG - chr5.1:77818982-77819001 None:intergenic 50.0%
! TGGCGAGCCATAGGTATTGT+CGG - chr5.1:77819164-77819183 None:intergenic 50.0%
!! TGAGTGCTGACACTCTCATC+TGG - chr5.1:77820012-77820031 None:intergenic 50.0%
ACATCAGCTTATGCCCGTGG+AGG + chr5.1:77819817-77819836 MS.gene38062:CDS 55.0%
ACCTATGGCTCGCCAATGAG+TGG + chr5.1:77819169-77819188 MS.gene38062:CDS 55.0%
CCACCACACATCCGGATTAC+AGG + chr5.1:77819781-77819800 MS.gene38062:CDS 55.0%
CCACGGGCATAAGCTGATGT+CGG - chr5.1:77819817-77819836 None:intergenic 55.0%
CCTGTAATCCGGATGTGTGG+TGG - chr5.1:77819784-77819803 None:intergenic 55.0%
GTGTCACGACTTTCGGGGAT+AGG - chr5.1:77820071-77820090 None:intergenic 55.0%
GTTGCACGGTTGTCTGCATC+CGG + chr5.1:77819523-77819542 MS.gene38062:CDS 55.0%
TCCACTCATTGGCGAGCCAT+AGG - chr5.1:77819173-77819192 None:intergenic 55.0%
TGGAACGACACGAGCTGCTA+GGG + chr5.1:77820286-77820305 MS.gene38062:CDS 55.0%
TTGCACGGTTGTCTGCATCC+GGG + chr5.1:77819524-77819543 MS.gene38062:CDS 55.0%
TTGTGAAGGGGTCGAGAGAG+TGG + chr5.1:77820266-77820285 MS.gene38062:CDS 55.0%
! ATAACCTTGCGGTTCGGCCA+GGG + chr5.1:77819944-77819963 MS.gene38062:CDS 55.0%
! CGAGCCATAGGTATTGTCGG+GGG - chr5.1:77819161-77819180 None:intergenic 55.0%
! GCGAGCCATAGGTATTGTCG+GGG - chr5.1:77819162-77819181 None:intergenic 55.0%
! GGCGAGCCATAGGTATTGTC+GGG - chr5.1:77819163-77819182 None:intergenic 55.0%
ACGAGCTGCTAGGGAAGTGG+AGG + chr5.1:77820295-77820314 MS.gene38062:CDS 60.0%
CCGACATCAGCTTATGCCCG+TGG + chr5.1:77819814-77819833 MS.gene38062:CDS 60.0%
CTGCACGTTGGAGTCGTCCT+TGG + chr5.1:77819024-77819043 MS.gene38062:CDS 60.0%
GACACGAGCTGCTAGGGAAG+TGG + chr5.1:77820292-77820311 MS.gene38062:CDS 60.0%
GAGAGAAGCTCGGCACTCTC+TGG - chr5.1:77819583-77819602 None:intergenic 60.0%
GTGGAACGACACGAGCTGCT+AGG + chr5.1:77820285-77820304 MS.gene38062:CDS 60.0%
TCAGCTTATGCCCGTGGAGG+TGG + chr5.1:77819820-77819839 MS.gene38062:CDS 60.0%
TTGGAGTCGTCCTTGGCAGC+TGG + chr5.1:77819031-77819050 MS.gene38062:CDS 60.0%
TTTACGGCCAGAGCTTCCCC+TGG - chr5.1:77819964-77819983 None:intergenic 60.0%
! AGGCTTTTGCAGCTAGGCCC+GGG + chr5.1:77819755-77819774 MS.gene38062:CDS 60.0%
! CAGGCTTTTGCAGCTAGGCC+CGG + chr5.1:77819754-77819773 MS.gene38062:CDS 60.0%
! CATAACCTTGCGGTTCGGCC+AGG + chr5.1:77819943-77819962 MS.gene38062:CDS 60.0%
! TAACCTTGCGGTTCGGCCAG+GGG + chr5.1:77819945-77819964 MS.gene38062:CDS 60.0%
!! CGGCAGATGGTGTCGGTTTC+TGG + chr5.1:77819457-77819476 MS.gene38062:CDS 60.0%
! GCTTTTGCAGCTAGGCCCGG+GGG + chr5.1:77819757-77819776 MS.gene38062:CDS 65.0%
! GGCTTTTGCAGCTAGGCCCG+GGG + chr5.1:77819756-77819775 MS.gene38062:CDS 65.0%
CCGGATGTGTGGTGGTCCCC+CGG - chr5.1:77819776-77819795 None:intergenic 70.0%
CCGGGGGACCACCACACATC+CGG + chr5.1:77819773-77819792 MS.gene38062:CDS 70.0%
CGGATGTGTGGTGGTCCCCC+GGG - chr5.1:77819775-77819794 None:intergenic 70.0%
CTTCCCCTGGCCGAACCGCA+AGG - chr5.1:77819951-77819970 None:intergenic 70.0%
GGTTCGGCCAGGGGAAGCTC+TGG + chr5.1:77819954-77819973 MS.gene38062:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 77818854 77820491 77818854 ID=MS.gene38062
chr5.1 mRNA 77818854 77820491 77818854 ID=MS.gene38062.t1;Parent=MS.gene38062
chr5.1 exon 77818854 77820491 77818854 ID=MS.gene38062.t1.exon1;Parent=MS.gene38062.t1
chr5.1 CDS 77818854 77820491 77818854 ID=cds.MS.gene38062.t1;Parent=MS.gene38062.t1
Gene Sequence

>MS.gene38062

ATGATGCAAGCATCAGAGCAGCATAGAAGTTCAAGTATGTACTATCAACCATTGCAGCAAATTGAACCCTATTGCTTGCCGCAATATCGAAATCTAAATCATCAGCTGTACAACAATGATGGAGGCCAAGCAACTCAATTCTCGGCTCCAACTTCTTCTGACCTTTATTACTGCACGTTGGAGTCGTCCTTGGCAGCTGGAAATTTTGTTGTTTACAACTCTCCTTCGACTGTTAGTTTCTCGCCTAACGATAGTCCAATGTCGCAGCAAGAATCTCAGTCTTATCCATCTGATAAGTATCATTCCCCCGACAATACCTATGGCTCGCCAATGAGTGGATCGTGCATAACCGAAGATTTAAGTAGCTTCAAGCACAAGCTTAGAGAATTGGAAAATGTAATGCTTGGTCCTGACTCTGATAATCTTGATAGTTACGACAGTGCTATCAGCAACGGAACCAATTTCGCTTCGCTTGAAATGGACAGTTGGAGACAAACAATGGTAGCAATTTCGAGCAAAAATCTAAAGCACATCCTTACTGCATGTGCCAAAGCAATTGCAGACAATGATTTGCTAATGGCGCAATGGTTGATGGACGAATTACGGCAGATGGTGTCGGTTTCTGGTGAACCGATTCAAAGATTGGGAGCATACATGTTGGAAGGACTCGTTGCACGGTTGTCTGCATCCGGGAGTTCAATCTACAAATCTTTAAGATGCAAAGAACCAGAGAGTGCCGAGCTTCTCTCTTACATGAACATACTTTACGAGGTTTGCCCTTACTTCAAATTTGGTTACATGTCTGCAAATGGAGCCATTGCAGAAGCCATGAAAAATGAAGCCAGAGTTCATATAATCGATTTCCAAATTGCTCAAGGAAGCCAATGGATTTCGTTGATTCAGGCTTTTGCAGCTAGGCCCGGGGGACCACCACACATCCGGATTACAGGTATTGATGATCCGACATCAGCTTATGCCCGTGGAGGTGGACTACATATAGTTGAAAAGAGGTTATCAAAGCTTGCTCGGCATTTCAAGGTGCCATTTGAGTTTCACGCTGCAGCTATCTCTGGTTGTGATGTTCAACTGCATAACCTTGCGGTTCGGCCAGGGGAAGCTCTGGCCGTAAATTTCGCATTCATGCTACATCACATGCCAGATGAGAGTGTCAGCACTCAGAATCATAGGGATAGGCTTTTAAGGTTGGTTAAGAGCCTATCCCCGAAAGTCGTGACACTTGTTGAGCAAGAATCTAACACAAACACTGCGGCATTCTTTCCACGTTTTCTCGAAACTATGGACTATTACACAGCAATGTTCGAGTCAATAGACGTGACTCTTCCGAGAGAGCATAAAGAGAGGATCAATGTTGAGCAGCATTGTTTGGCAAGAGATTTAGTTAACATCATAGCTTGTGAAGGGGTCGAGAGAGTGGAACGACACGAGCTGCTAGGGAAGTGGAGGTCAAGATTTGCAATGGCTGGTTTCACTCCTTACCCTCTGAGTTCTTTGGTGAATGGTACCATAAAGAAATTGCTTGAAAACTACAGTGATAGATATAGACTTGAAGAGAGAGATGGAGCTCTATATCTTGGTTGGATGAATAGAGATTTGGTTGCTTCTTGTGCTTGGAAATGA
Protein sequence

>MS.gene38062.t1

MMQASEQHRSSSMYYQPLQQIEPYCLPQYRNLNHQLYNNDGGQATQFSAPTSSDLYYCTLESSLAAGNFVVYNSPSTVSFSPNDSPMSQQESQSYPSDKYHSPDNTYGSPMSGSCITEDLSSFKHKLRELENVMLGPDSDNLDSYDSAISNGTNFASLEMDSWRQTMVAISSKNLKHILTACAKAIADNDLLMAQWLMDELRQMVSVSGEPIQRLGAYMLEGLVARLSASGSSIYKSLRCKEPESAELLSYMNILYEVCPYFKFGYMSANGAIAEAMKNEARVHIIDFQIAQGSQWISLIQAFAARPGGPPHIRITGIDDPTSAYARGGGLHIVEKRLSKLARHFKVPFEFHAAAISGCDVQLHNLAVRPGEALAVNFAFMLHHMPDESVSTQNHRDRLLRLVKSLSPKVVTLVEQESNTNTAAFFPRFLETMDYYTAMFESIDVTLPREHKERINVEQHCLARDLVNIIACEGVERVERHELLGKWRSRFAMAGFTPYPLSSLVNGTIKKLLENYSDRYRLEERDGALYLGWMNRDLVASCAWK