AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38065

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38065.t1 MTR_5g097500 81.994 311 23 2 1 278 1 311 0.0 513
MS.gene38065.t1 MTR_5g097490 50.915 328 106 4 1 273 1 328 2.92e-104 309
MS.gene38065.t1 MTR_4g021780 73.737 99 25 1 71 169 15 112 1.87e-49 159
MS.gene38065.t1 MTR_4g013260 33.778 225 113 3 73 261 176 400 1.41e-37 139
MS.gene38065.t1 MTR_7g071120 33.740 246 117 5 72 271 158 403 1.85e-37 138
MS.gene38065.t1 MTR_3g056100 30.104 289 153 3 23 264 39 325 3.40e-36 134
MS.gene38065.t1 MTR_3g056100 30.104 289 153 3 23 264 183 469 1.99e-35 134
MS.gene38065.t1 MTR_5g094430 32.083 240 117 3 80 273 67 306 4.81e-35 130
MS.gene38065.t1 MTR_5g094430 43.478 69 36 2 189 256 58 124 3.57e-11 63.5
MS.gene38065.t1 MTR_5g094430 32.083 240 117 3 80 273 89 328 5.69e-35 130
MS.gene38065.t1 MTR_5g094430 43.478 69 36 2 189 256 80 146 3.39e-11 63.5
MS.gene38065.t1 MTR_5g094430 32.083 240 117 3 80 273 201 440 1.22e-34 131
MS.gene38065.t1 MTR_5g094430 43.478 69 36 2 189 256 192 258 4.50e-11 63.2
MS.gene38065.t1 MTR_3g056100 28.808 302 153 4 23 264 39 338 1.67e-34 129
MS.gene38065.t1 MTR_3g056100 28.808 302 153 4 23 264 183 482 7.60e-34 129
MS.gene38065.t1 MTR_5g074400 31.707 246 109 3 80 266 244 489 2.31e-33 128
MS.gene38065.t1 MTR_3g031220 31.780 236 112 5 80 266 199 434 9.27e-33 125
MS.gene38065.t1 MTR_4g082580 28.417 278 184 5 15 277 81 358 1.44e-32 123
MS.gene38065.t1 MTR_7g100510 28.920 287 132 7 27 257 151 421 4.73e-30 117
MS.gene38065.t1 MTR_3g009470 35.246 122 68 2 153 263 440 561 2.66e-19 87.8
MS.gene38065.t1 MTR_3g009470 50.847 59 29 0 79 137 280 338 1.77e-14 73.6
MS.gene38065.t1 MTR_7g080020 43.333 90 51 0 182 271 478 567 4.79e-19 87.0
MS.gene38065.t1 MTR_7g080020 47.945 73 35 1 79 148 280 352 2.29e-15 76.3
MS.gene38065.t1 MTR_5g042920 41.379 87 51 0 182 268 305 391 7.95e-19 85.9
MS.gene38065.t1 MTR_5g042920 40.000 85 49 1 55 137 127 211 1.55e-12 67.4
MS.gene38065.t1 MTR_5g042920 38.043 92 54 2 187 276 139 229 1.32e-11 64.7
MS.gene38065.t1 MTR_7g080000 48.000 75 39 0 185 259 420 494 1.00e-18 86.3
MS.gene38065.t1 MTR_7g080000 46.774 62 33 0 79 140 234 295 5.10e-14 72.4
MS.gene38065.t1 MTR_7g011820 40.230 87 52 0 182 268 395 481 2.92e-18 84.7
MS.gene38065.t1 MTR_7g011820 50.847 59 29 0 80 138 229 287 3.34e-14 72.8
MS.gene38065.t1 MTR_7g011820 46.774 62 30 2 196 256 227 286 3.73e-11 63.5
MS.gene38065.t1 MTR_3g031220 29.670 182 79 5 80 212 199 380 2.49e-17 81.3
MS.gene38065.t1 MTR_1g015140 49.275 69 35 0 185 253 90 158 5.71e-16 73.9
MS.gene38065.t1 MTR_3g104750 47.826 69 36 0 185 253 92 160 1.11e-15 73.2
MS.gene38065.t1 MTR_4g130900 45.333 75 41 0 185 259 347 421 2.50e-15 75.9
MS.gene38065.t1 MTR_8g092010 43.836 73 41 0 185 257 96 168 4.49e-15 72.0
MS.gene38065.t1 MTR_8g096730 35.870 92 51 2 186 271 59 148 1.19e-14 70.1
MS.gene38065.t1 MTR_3g009470 50.847 59 29 0 79 137 280 338 2.27e-14 73.2
MS.gene38065.t1 MTR_7g079010 36.264 91 58 0 163 253 107 197 2.78e-14 70.5
MS.gene38065.t1 MTR_7g079010 41.176 68 37 2 69 135 133 198 5.30e-11 61.2
MS.gene38065.t1 MTR_7g109800 44.737 76 42 0 182 257 130 205 2.98e-14 71.6
MS.gene38065.t1 MTR_1g077780 37.333 75 47 0 182 256 47 121 3.49e-14 68.6
MS.gene38065.t1 MTR_4g094588 47.222 72 38 0 185 256 166 237 8.73e-14 70.9
MS.gene38065.t1 MTR_8g007270 43.243 74 42 0 182 255 139 212 1.02e-13 69.3
MS.gene38065.t1 MTR_2g033820 44.000 75 42 0 182 256 188 262 1.10e-13 70.9
MS.gene38065.t1 MTR_2g033820 41.935 62 35 1 80 140 204 265 5.79e-11 62.8
MS.gene38065.t1 MTR_7g028415 44.118 68 38 0 186 253 82 149 1.94e-13 67.4
MS.gene38065.t1 MTR_7g028710 44.118 68 38 0 186 253 82 149 1.94e-13 67.4
MS.gene38065.t1 MTR_3g095040 42.466 73 42 0 185 257 106 178 3.59e-13 67.4
MS.gene38065.t1 MTR_1g086790 44.000 75 42 0 182 256 143 217 3.84e-13 68.9
MS.gene38065.t1 MTR_5g043880 47.826 69 36 0 185 253 112 180 4.25e-13 68.2
MS.gene38065.t1 MTR_5g074200 33.333 141 86 1 1 141 150 282 5.38e-13 68.6
MS.gene38065.t1 MTR_2g105060 36.905 84 52 1 174 256 71 154 1.05e-12 66.6
MS.gene38065.t1 MTR_2g105060 42.254 71 39 2 69 137 84 154 9.99e-11 60.8
MS.gene38065.t1 MTR_5g091390 44.444 72 40 0 185 256 145 216 1.36e-12 67.0
MS.gene38065.t1 MTR_2g105060 36.905 84 52 1 174 256 146 229 1.81e-12 66.6
MS.gene38065.t1 MTR_3g095080 41.892 74 43 0 182 255 129 202 2.10e-12 65.5
MS.gene38065.t1 MTR_4g007060 39.773 88 44 3 188 266 119 206 2.14e-12 66.2
MS.gene38065.t1 MTR_4g007060 39.773 88 44 3 188 266 153 240 2.45e-12 66.2
MS.gene38065.t1 MTR_3g106060 44.444 72 37 2 185 253 124 195 3.05e-12 65.9
MS.gene38065.t1 MTR_3g095080 41.892 74 43 0 182 255 153 226 3.15e-12 65.1
MS.gene38065.t1 MTR_1g013760 47.887 71 35 2 185 253 127 197 6.72e-12 64.7
MS.gene38065.t1 MTR_4g122530 39.726 73 44 0 185 257 196 268 7.00e-12 65.5
MS.gene38065.t1 MTR_7g110720 36.709 79 48 2 187 263 179 257 1.00e-11 65.1
MS.gene38065.t1 MTR_7g110720 36.709 79 48 2 187 263 206 284 1.21e-11 64.7
MS.gene38065.t1 MTR_4g094412 41.538 65 37 1 195 258 232 296 1.99e-11 64.3
MS.gene38065.t1 MTR_4g094412 41.538 65 37 1 195 258 241 305 2.01e-11 64.3
MS.gene38065.t1 MTR_1g099600 37.234 94 53 2 191 278 179 272 2.21e-11 63.5
MS.gene38065.t1 MTR_3g053330 25.294 170 111 4 108 276 177 331 2.30e-11 63.9
MS.gene38065.t1 MTR_4g049790 37.333 75 46 1 185 258 300 374 2.63e-11 63.9
MS.gene38065.t1 MTR_8g092140 45.161 62 34 0 194 255 157 218 2.82e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38065.t1 AT2G04880 30.816 331 162 5 1 277 46 363 2.05e-43 154
MS.gene38065.t1 AT5G07100 36.098 205 119 5 61 259 5 203 2.75e-35 126
MS.gene38065.t1 AT5G07100 36.098 205 119 5 61 259 5 203 2.75e-35 126
MS.gene38065.t1 AT5G07100 36.098 205 119 5 61 259 5 203 2.75e-35 126
MS.gene38065.t1 AT5G07100 36.098 205 119 5 61 259 5 203 2.75e-35 126
MS.gene38065.t1 AT5G07100 36.098 205 119 5 61 259 98 296 1.59e-34 127
MS.gene38065.t1 AT3G01080 35.354 198 107 3 80 256 168 365 8.87e-34 127
MS.gene38065.t1 AT3G01080 35.354 198 107 3 80 256 206 403 1.44e-33 127
MS.gene38065.t1 AT2G03340 31.933 238 115 3 80 270 251 488 8.92e-33 125
MS.gene38065.t1 AT2G03340 42.857 84 47 1 188 271 241 323 6.07e-15 74.7
MS.gene38065.t1 AT2G30250 33.484 221 103 4 80 256 167 387 8.91e-32 121
MS.gene38065.t1 AT4G30935 28.159 277 145 6 27 257 123 391 1.36e-25 105
MS.gene38065.t1 AT2G38470 29.218 243 110 5 79 260 184 425 1.41e-23 100
MS.gene38065.t1 AT2G04880 36.486 148 84 2 140 277 240 387 1.85e-23 99.8
MS.gene38065.t1 AT2G04880 34.965 143 80 2 1 143 46 175 3.20e-18 84.3
MS.gene38065.t1 AT2G04880 44.118 68 37 1 194 261 108 174 5.82e-13 68.9
MS.gene38065.t1 AT5G56270 47.436 78 41 0 182 259 472 549 8.84e-20 89.4
MS.gene38065.t1 AT5G56270 45.763 59 32 0 79 137 273 331 1.19e-11 65.1
MS.gene38065.t1 AT1G13960 45.333 75 41 0 182 256 241 315 1.74e-18 84.3
MS.gene38065.t1 AT1G13960 46.575 73 38 1 184 256 63 134 9.09e-15 73.9
MS.gene38065.t1 AT1G13960 50.820 61 30 0 80 140 77 137 2.35e-13 69.7
MS.gene38065.t1 AT1G13960 45.333 75 41 0 182 256 394 468 2.15e-18 85.1
MS.gene38065.t1 AT1G13960 46.575 73 38 1 184 256 216 287 8.20e-15 74.3
MS.gene38065.t1 AT1G13960 50.820 61 30 0 80 140 230 290 1.28e-13 70.9
MS.gene38065.t1 AT4G26440 45.122 82 45 0 182 263 357 438 3.91e-17 81.6
MS.gene38065.t1 AT4G26440 50.000 58 29 0 80 137 179 236 1.04e-12 68.2
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 190 267 8.32e-17 79.7
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 18 94 3.21e-15 75.5
MS.gene38065.t1 AT4G26640 44.444 63 32 2 195 256 33 93 7.46e-11 62.4
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 190 267 8.32e-17 79.7
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 18 94 3.21e-15 75.5
MS.gene38065.t1 AT4G26640 44.444 63 32 2 195 256 33 93 7.46e-11 62.4
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 190 267 8.32e-17 79.7
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 18 94 3.21e-15 75.5
MS.gene38065.t1 AT4G26640 44.444 63 32 2 195 256 33 93 7.46e-11 62.4
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 190 267 8.32e-17 79.7
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 18 94 3.21e-15 75.5
MS.gene38065.t1 AT4G26640 44.444 63 32 2 195 256 33 93 7.46e-11 62.4
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 326 403 9.86e-17 80.1
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 154 230 1.71e-15 76.6
MS.gene38065.t1 AT4G26640 42.647 68 36 2 190 256 164 229 6.69e-11 62.8
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 294 371 1.06e-16 80.1
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 122 198 2.32e-15 76.3
MS.gene38065.t1 AT4G26640 42.647 68 36 2 190 256 132 197 7.28e-11 62.8
MS.gene38065.t1 AT4G26640 44.872 78 43 0 182 259 366 443 1.09e-16 80.1
MS.gene38065.t1 AT4G26640 44.156 77 42 1 63 138 194 270 2.06e-15 76.3
MS.gene38065.t1 AT4G26640 42.647 68 36 2 190 256 204 269 7.26e-11 62.8
MS.gene38065.t1 AT5G13080 45.588 68 37 0 186 253 56 123 3.51e-15 71.6
MS.gene38065.t1 AT5G64810 43.662 71 40 0 185 255 98 168 5.75e-15 72.0
MS.gene38065.t1 AT5G26170 46.377 69 37 0 185 253 101 169 8.74e-15 71.2
MS.gene38065.t1 AT3G01080 52.542 59 28 0 80 138 206 264 1.48e-14 72.8
MS.gene38065.t1 AT1G55600 45.833 72 39 0 185 256 295 366 3.35e-14 72.8
MS.gene38065.t1 AT2G47260 43.421 76 43 0 182 257 159 234 4.98e-14 71.6
MS.gene38065.t1 AT2G21900 40.789 76 43 1 183 256 93 168 7.00e-14 69.3
MS.gene38065.t1 AT5G49520 44.737 76 42 0 182 257 206 281 7.55e-14 71.2
MS.gene38065.t1 AT2G44745 43.243 74 42 0 182 255 130 203 2.17e-13 68.2
MS.gene38065.t1 AT2G44745 43.662 71 39 1 77 146 143 213 2.35e-11 62.4
MS.gene38065.t1 AT1G69310 45.714 70 38 0 185 254 135 204 3.07e-13 68.9
MS.gene38065.t1 AT1G69310 45.714 70 38 0 185 254 135 204 3.07e-13 68.9
MS.gene38065.t1 AT1G69310 45.714 70 38 0 185 254 135 204 3.07e-13 68.9
MS.gene38065.t1 AT3G62340 48.387 62 32 0 196 257 117 178 5.66e-13 67.8
MS.gene38065.t1 AT5G41570 43.478 69 39 0 185 253 86 154 7.10e-13 65.9
MS.gene38065.t1 AT5G41570 45.000 60 32 1 77 135 96 155 5.58e-11 60.8
MS.gene38065.t1 AT2G25000 37.931 87 50 2 184 266 90 176 7.34e-13 67.0
MS.gene38065.t1 AT2G25000 37.931 87 50 2 184 266 90 176 7.34e-13 67.0
MS.gene38065.t1 AT2G25000 37.931 87 50 2 184 266 112 198 9.42e-13 67.0
MS.gene38065.t1 AT1G64000 43.478 69 39 0 185 253 102 170 9.89e-13 65.9
MS.gene38065.t1 AT1G64000 45.000 60 32 1 77 135 112 171 2.22e-11 62.0
MS.gene38065.t1 AT2G25000 37.931 87 50 2 184 266 133 219 1.25e-12 67.0
MS.gene38065.t1 AT1G29860 42.667 75 43 0 182 256 121 195 1.62e-12 66.6
MS.gene38065.t1 AT4G18170 45.333 75 41 0 182 256 157 231 1.78e-12 67.0
MS.gene38065.t1 AT3G01970 42.647 68 39 0 186 253 54 121 2.29e-12 63.9
MS.gene38065.t1 AT2G37260 50.000 58 29 0 80 137 166 223 2.62e-12 67.0
MS.gene38065.t1 AT2G37260 39.706 68 41 0 186 253 338 405 9.95e-12 65.1
MS.gene38065.t1 AT4G39410 43.243 74 42 0 182 255 208 281 3.75e-12 65.9
MS.gene38065.t1 AT2G46130 41.791 67 39 0 187 253 20 86 4.22e-12 62.0
MS.gene38065.t1 AT4G23550 41.333 75 43 1 186 259 129 203 4.41e-12 65.5
MS.gene38065.t1 AT2G37260 50.000 58 29 0 80 137 86 143 5.22e-12 65.9
MS.gene38065.t1 AT2G37260 39.706 68 41 0 186 253 258 325 7.67e-12 65.1
MS.gene38065.t1 AT2G37260 50.000 58 29 0 80 137 84 141 5.63e-12 65.5
MS.gene38065.t1 AT2G37260 39.706 68 41 0 186 253 256 323 7.97e-12 65.1
MS.gene38065.t1 AT5G46350 48.438 64 33 0 196 259 182 245 5.81e-12 65.5
MS.gene38065.t1 AT4G01720 43.662 71 39 1 187 256 229 299 6.78e-12 65.9
MS.gene38065.t1 AT4G31800 38.667 75 45 1 183 256 97 171 1.54e-11 63.5
MS.gene38065.t1 AT1G68150 31.111 90 61 1 187 275 225 314 2.03e-11 63.9
MS.gene38065.t1 AT4G31800 38.667 75 45 1 183 256 162 236 2.45e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.16e-11 64.3
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 5.10e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.17e-11 64.3
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 5.55e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.17e-11 64.3
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 5.55e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.25e-11 64.3
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 5.05e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 388 459 3.43e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 364 459 6.22e-11 63.2
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.47e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 5.75e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.50e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 6.02e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.55e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 6.27e-11 63.2
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.55e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 6.04e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.55e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 6.04e-11 63.5
MS.gene38065.t1 AT4G12020 41.096 73 42 1 184 256 455 526 3.96e-11 63.9
MS.gene38065.t1 AT4G12020 35.417 96 53 1 51 137 431 526 8.87e-11 62.8
MS.gene38065.t1 AT4G04450 34.066 91 59 1 187 276 188 278 9.64e-11 62.4

Find 52 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATAATGCGTATTAA+TGG 0.253581 5.1:-77842054 MS.gene38065:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5.1:-77842136 MS.gene38065:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5.1:+77842122 None:intergenic
ACTTCCACACCGGTCAAATA+TGG 0.326583 5.1:+77842623 None:intergenic
TGTTCTTCCCGTTGTTGAAC+AGG 0.349804 5.1:-77842657 MS.gene38065:CDS
AATTCATGTGAAGGAGTATT+AGG 0.352642 5.1:+77842941 None:intergenic
ACAATGCTTGATTGAGGATT+AGG 0.367320 5.1:+77842695 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5.1:+77842150 None:intergenic
ACTAGAGTTGGCGTGTTAAC+TGG 0.387351 5.1:-77842495 MS.gene38065:CDS
TGAATGTGTGCATTTGTAAT+AGG 0.403229 5.1:+77842799 None:intergenic
AAATTTCATTGGTCAAATGA+TGG 0.419177 5.1:-77842758 MS.gene38065:CDS
GCTTATCGCTGGCGCAAATA+CGG 0.420523 5.1:-77842420 MS.gene38065:CDS
TACTTTCGATGCTCCTATCC+AGG 0.428745 5.1:-77842204 MS.gene38065:CDS
TCGCTCAATCATACGTGAAA+TGG 0.436400 5.1:-77842891 MS.gene38065:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5.1:-77842135 MS.gene38065:CDS
CGTGAAATGGTAAGAAAAGA+TGG 0.461858 5.1:-77842878 MS.gene38065:CDS
ACGGGAAGAACATGATCAAC+TGG 0.472828 5.1:+77842668 None:intergenic
TGTGGTCCCTGTTCAACAAC+GGG 0.491578 5.1:+77842650 None:intergenic
TTGACCGGTGTGGAAGTTCA+AGG 0.496310 5.1:-77842617 MS.gene38065:intron
AATTTCATTGGTCAAATGAT+GGG 0.502281 5.1:-77842757 MS.gene38065:CDS
TTTCACTGTGCATCCTGGAT+AGG 0.505699 5.1:+77842191 None:intergenic
AGAAACTAATGATGTAGCTA+TGG 0.532105 5.1:-77843005 MS.gene38065:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5.1:+77842151 None:intergenic
TAAGAAAAGATGGCTATAAG+TGG 0.538492 5.1:-77842868 MS.gene38065:CDS
AATACTGATGCTACTAGAGT+TGG 0.539414 5.1:-77842507 MS.gene38065:CDS
TGACGGACACCCCTACCAAT+CGG 0.549476 5.1:+77842093 None:intergenic
GTTCTTCCCGTTGTTGAACA+GGG 0.552964 5.1:-77842656 MS.gene38065:CDS
TCATGAACCACCGATTGGTA+GGG 0.553339 5.1:-77842103 MS.gene38065:CDS
CCACATCAACCATATTTGAC+CGG 0.557825 5.1:-77842632 MS.gene38065:CDS
ATCATGAACCACCGATTGGT+AGG 0.562327 5.1:-77842104 MS.gene38065:CDS
ACTCGATGTTGTACTTCAGA+AGG 0.565037 5.1:+77843058 None:intergenic
CATGAACCACCGATTGGTAG+GGG 0.570838 5.1:-77842102 MS.gene38065:CDS
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5.1:-77842993 MS.gene38065:CDS
TATTGATTAGATTGTATCAG+AGG 0.571636 5.1:+77842917 None:intergenic
TTGTAAATGATGCTTATCGC+TGG 0.587731 5.1:-77842431 MS.gene38065:CDS
CATGATCATGAACCACCGAT+TGG 0.591424 5.1:-77842108 MS.gene38065:CDS
ATCAGAGGTAATTCATGTGA+AGG 0.594434 5.1:+77842932 None:intergenic
CCGGTCAAATATGGTTGATG+TGG 0.605641 5.1:+77842632 None:intergenic
ATGTGGTCCCTGTTCAACAA+CGG 0.606165 5.1:+77842649 None:intergenic
GGTGGTACAATGCTTGATTG+AGG 0.614087 5.1:+77842689 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5.1:+77842152 None:intergenic
ACATCGAGTGAATCCTCAGA+GGG 0.633099 5.1:-77843044 MS.gene38065:CDS
GACAATGTGATCTCCCTCTG+AGG 0.634875 5.1:+77843031 None:intergenic
CACATATGCAACACTCGCAG+AGG 0.656505 5.1:-77842964 MS.gene38065:CDS
TATTTGTAACAGAGTCATGA+CGG 0.659620 5.1:+77842076 None:intergenic
AGTGTAACTGAAATATTCGA+CGG 0.667161 5.1:+77842733 None:intergenic
ATCACCTTGAACTTCCACAC+CGG 0.673408 5.1:+77842613 None:intergenic
CACTTGAAGTCTGAACAACA+AGG 0.680480 5.1:+77842463 None:intergenic
TCAACCATATTTGACCGGTG+TGG 0.682556 5.1:-77842627 MS.gene38065:CDS
GGAAGAACATGATCAACTGG+TGG 0.704738 5.1:+77842671 None:intergenic
CGGACACCCCTACCAATCGG+TGG 0.708398 5.1:+77842096 None:intergenic
AACATCGAGTGAATCCTCAG+AGG 0.727570 5.1:-77843045 MS.gene38065:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG + chr5.1:77842598-77842617 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG - chr5.1:77842755-77842774 MS.gene38065:CDS 20.0%
! AAATTTCATTGGTCAAATGA+TGG - chr5.1:77842383-77842402 MS.gene38065:intron 25.0%
! AATGTAACAACTACCTATAA+GGG - chr5.1:77843006-77843025 MS.gene38065:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG - chr5.1:77842384-77842403 MS.gene38065:intron 25.0%
! ACTCAAATAATGCGTATTAA+TGG - chr5.1:77843087-77843106 MS.gene38065:CDS 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG + chr5.1:77842786-77842805 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG - chr5.1:77842372-77842391 MS.gene38065:intron 25.0%
! TAATGTAACAACTACCTATA+AGG - chr5.1:77843005-77843024 MS.gene38065:CDS 25.0%
! TTACATTAGTCACATTTAGA+GGG + chr5.1:77842993-77843012 None:intergenic 25.0%
!! TATTGATTAGATTGTATCAG+AGG + chr5.1:77842227-77842246 None:intergenic 25.0%
AGAAACTAATGATGTAGCTA+TGG - chr5.1:77842136-77842155 MS.gene38065:CDS 30.0%
AGTGTAACTGAAATATTCGA+CGG + chr5.1:77842411-77842430 None:intergenic 30.0%
GGCTATAAGTGGAAAAAATA+TGG - chr5.1:77842284-77842303 MS.gene38065:intron 30.0%
GTTACATTAGTCACATTTAG+AGG + chr5.1:77842994-77843013 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG - chr5.1:77842273-77842292 MS.gene38065:intron 30.0%
TACATTAGTCACATTTAGAG+GGG + chr5.1:77842992-77843011 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG - chr5.1:77842739-77842758 MS.gene38065:CDS 30.0%
TATTTGTAACAGAGTCATGA+CGG + chr5.1:77843068-77843087 None:intergenic 30.0%
TGAATGTGTGCATTTGTAAT+AGG + chr5.1:77842345-77842364 None:intergenic 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG + chr5.1:77842924-77842943 None:intergenic 30.0%
!! AATTCATGTGAAGGAGTATT+AGG + chr5.1:77842203-77842222 None:intergenic 30.0%
AATACTGATGCTACTAGAGT+TGG - chr5.1:77842634-77842653 MS.gene38065:CDS 35.0%
ACAATGCTTGATTGAGGATT+AGG + chr5.1:77842449-77842468 None:intergenic 35.0%
ATCAGAGGTAATTCATGTGA+AGG + chr5.1:77842212-77842231 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG + chr5.1:77843022-77843041 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG - chr5.1:77842263-77842282 MS.gene38065:intron 35.0%
TTGTAAATGATGCTTATCGC+TGG - chr5.1:77842710-77842729 MS.gene38065:CDS 35.0%
! AGTAATTTCTGGAAGATTGG+AGG + chr5.1:77842921-77842940 None:intergenic 35.0%
! GTAATTTCTGGAAGATTGGA+GGG + chr5.1:77842920-77842939 None:intergenic 35.0%
ACTCGATGTTGTACTTCAGA+AGG + chr5.1:77842086-77842105 None:intergenic 40.0%
CACTTGAAGTCTGAACAACA+AGG + chr5.1:77842681-77842700 None:intergenic 40.0%
CCACATCAACCATATTTGAC+CGG - chr5.1:77842509-77842528 MS.gene38065:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG - chr5.1:77842732-77842751 MS.gene38065:CDS 40.0%
TCGCTCAATCATACGTGAAA+TGG - chr5.1:77842250-77842269 MS.gene38065:intron 40.0%
!! GGAGCATCGAAAGTAATTTC+TGG + chr5.1:77842932-77842951 None:intergenic 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG + chr5.1:77842958-77842977 None:intergenic 40.0%
AACATCGAGTGAATCCTCAG+AGG - chr5.1:77842096-77842115 MS.gene38065:CDS 45.0%
ACATCGAGTGAATCCTCAGA+GGG - chr5.1:77842097-77842116 MS.gene38065:CDS 45.0%
ACGGGAAGAACATGATCAAC+TGG + chr5.1:77842476-77842495 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG - chr5.1:77842646-77842665 MS.gene38065:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG + chr5.1:77842521-77842540 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG + chr5.1:77842531-77842550 None:intergenic 45.0%
ATGTGGTCCCTGTTCAACAA+CGG + chr5.1:77842495-77842514 None:intergenic 45.0%
CATGATCATGAACCACCGAT+TGG - chr5.1:77843033-77843052 MS.gene38065:CDS 45.0%
GGAAGAACATGATCAACTGG+TGG + chr5.1:77842473-77842492 None:intergenic 45.0%
GGTGGTACAATGCTTGATTG+AGG + chr5.1:77842455-77842474 None:intergenic 45.0%
GTTCTTCCCGTTGTTGAACA+GGG - chr5.1:77842485-77842504 MS.gene38065:CDS 45.0%
TACTTTCGATGCTCCTATCC+AGG - chr5.1:77842937-77842956 MS.gene38065:CDS 45.0%
TCAACCATATTTGACCGGTG+TGG - chr5.1:77842514-77842533 MS.gene38065:CDS 45.0%
TGTTCTTCCCGTTGTTGAAC+AGG - chr5.1:77842484-77842503 MS.gene38065:CDS 45.0%
TTTCACTGTGCATCCTGGAT+AGG + chr5.1:77842953-77842972 None:intergenic 45.0%
! ATCATGAACCACCGATTGGT+AGG - chr5.1:77843037-77843056 MS.gene38065:CDS 45.0%
! CCGGTCAAATATGGTTGATG+TGG + chr5.1:77842512-77842531 None:intergenic 45.0%
! TCATGAACCACCGATTGGTA+GGG - chr5.1:77843038-77843057 MS.gene38065:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG - chr5.1:77842148-77842167 MS.gene38065:CDS 45.0%
CACATATGCAACACTCGCAG+AGG - chr5.1:77842177-77842196 MS.gene38065:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG + chr5.1:77842113-77842132 None:intergenic 50.0%
GCTTATCGCTGGCGCAAATA+CGG - chr5.1:77842721-77842740 MS.gene38065:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+GGG + chr5.1:77842494-77842513 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG - chr5.1:77842524-77842543 MS.gene38065:intron 50.0%
! CATGAACCACCGATTGGTAG+GGG - chr5.1:77843039-77843058 MS.gene38065:CDS 50.0%
! TGACGGACACCCCTACCAAT+CGG + chr5.1:77843051-77843070 None:intergenic 55.0%
CGGACACCCCTACCAATCGG+TGG + chr5.1:77843048-77843067 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 77842044 77843119 77842044 ID=MS.gene38065
chr5.1 mRNA 77842044 77843119 77842044 ID=MS.gene38065.t1;Parent=MS.gene38065
chr5.1 exon 77842618 77843119 77842618 ID=MS.gene38065.t1.exon1;Parent=MS.gene38065.t1
chr5.1 CDS 77842618 77843119 77842618 ID=cds.MS.gene38065.t1;Parent=MS.gene38065.t1
chr5.1 exon 77842387 77842534 77842387 ID=MS.gene38065.t1.exon2;Parent=MS.gene38065.t1
chr5.1 CDS 77842387 77842534 77842387 ID=cds.MS.gene38065.t1;Parent=MS.gene38065.t1
chr5.1 exon 77842044 77842230 77842044 ID=MS.gene38065.t1.exon3;Parent=MS.gene38065.t1
chr5.1 CDS 77842044 77842230 77842044 ID=cds.MS.gene38065.t1;Parent=MS.gene38065.t1
Gene Sequence

>MS.gene38065

ATGGTTTTGTCACTACAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACAACATCGAGTGAATCCTCAGAGGGAGATCACATTGTCACGAGAAACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATATGCAACACTCGCAGAGGCCTAATACTCCTTCACATGAATTACCTCTGATACAATCTAATCAATACACTCGCTCAATCATACGTGAAATGGTAAGAAAAGATGGCTATAAGTGGAAAAAATATGGTGAGAAAAACATTAAGAAGAATGAACATAAAAGAGCCTATTACAAATGCACACATTCAGACTGTCGTGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACCGTCGAATATTTCAGTTACACTAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACCAGTTGATCATGTTCTTCCCGTTGTTGAACAGGGACCACATCAACCATATTTGACCGGTGTGGAAGTTCAAGGTGATAAGTACTCTTTGTTAGTATTTTAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATACTGATGCTACTAGAGTTGGCGTGTTAACTGGTGAACTTCACCTTGTTGTTCAGACTTCAAGTGCAAATGAAGTTGTAAATGATGCTTATCGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGGTAAATCCTAGCTTTTATTTTGAACTCATACATTGTAGATCATTTCAATATGCATTTTTTTATTGCTTTTTCTCTATGTGCTTTAAGCTTTTGCAAATACACAAATTCATGAAATGATTTTATCTAATTGATTGCATTTTCCCTCCAATCTTCCAGAAATTACTTTCGATGCTCCTATCCAGGATGCACAGTGAAAAAGTATGTTGAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGATTGGTAGGGGTGTCCGTCATGACTCTGTTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>MS.gene38065.t1

MVLSLQNVDHNDSPSEVQHRVNPQREITLSRETNDVAMACDLVLSHMQHSQRPNTPSHELPLIQSNQYTRSIIREMVRKDGYKWKKYGEKNIKKNEHKRAYYKCTHSDCRAKKKFHWSNDGTVEYFSYTNQHNHPNPQSSIVPPVDHVLPVVEQGPHQPYLTGVEVQDNNTDATRVGVLTGELHLVVQTSSANEVVNDAYRWRKYGRKMVNGKTIQRNYFRCSYPGCTVKKYVEKSPLNVTNVTTTYKGQHDHEPPIGRGVRHDSVTNTQIMRINGEP