Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38130.t1 | XP_003618052.1 | 70.7 | 270 | 44 | 2 | 1 | 235 | 1 | 270 | 1.20E-88 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38130.t1 | G7K0M6 | 70.7 | 270 | 44 | 2 | 1 | 235 | 1 | 270 | 8.4e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene38130.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38130.t1 | MTR_5g098470 | 80.000 | 270 | 19 | 2 | 1 | 235 | 1 | 270 | 2.34e-149 | 417 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATATTATTATCCTTATT+AGG | 0.131951 | 5.1:+79147853 | None:intergenic |
| ATACCCCTCCATTCCCTTTC+AGG | 0.172402 | 5.1:+79147880 | None:intergenic |
| GTGACCATGTAAAGCCTTAT+TGG | 0.177322 | 5.1:+79147915 | None:intergenic |
| ATCAAAAGATAATGTCTTAT+TGG | 0.200998 | 5.1:-79147488 | MS.gene38130:CDS |
| ATCATCAATAACCAATTCTT+TGG | 0.220906 | 5.1:+79147783 | None:intergenic |
| CAATATTATTATCCTTATTA+GGG | 0.247792 | 5.1:+79147854 | None:intergenic |
| CATCAAACTTATTCTTCTTT+TGG | 0.256389 | 5.1:+79148328 | None:intergenic |
| AATAACCAATTCTTTGGAAT+TGG | 0.272420 | 5.1:+79147789 | None:intergenic |
| CGACAACTTCAATGAGGATT+TGG | 0.318999 | 5.1:-79147644 | MS.gene38130:CDS |
| TTTAGGTGTGGAAAAGGATT+AGG | 0.327731 | 5.1:-79148057 | MS.gene38130:intron |
| TGCATCCAATTCCAAAGAAT+TGG | 0.334606 | 5.1:-79147794 | MS.gene38130:CDS |
| CTTTCCCTAGAGCTTGACTA+TGG | 0.341322 | 5.1:+79148019 | None:intergenic |
| TTGATGATGAATCAAAATCT+TGG | 0.371768 | 5.1:-79147768 | MS.gene38130:CDS |
| GAGGGGTATGAACCCTAATA+AGG | 0.375613 | 5.1:-79147866 | MS.gene38130:CDS |
| TACCCCTCCATTCCCTTTCA+GGG | 0.375926 | 5.1:+79147881 | None:intergenic |
| AAAGAACTTCCAATCCAATA+AGG | 0.383910 | 5.1:-79147929 | MS.gene38130:CDS |
| GTTGTCGTGTAAATGTTTAT+CGG | 0.409444 | 5.1:+79147660 | None:intergenic |
| TGATGATGAATCAAAATCTT+GGG | 0.427675 | 5.1:-79147767 | MS.gene38130:CDS |
| CATGTAAAGCCTTATTGGAT+TGG | 0.438633 | 5.1:+79147920 | None:intergenic |
| TTTGGATGATGAGGATAAGA+AGG | 0.451348 | 5.1:-79147626 | MS.gene38130:CDS |
| CAATCCAATAAGGCTTTACA+TGG | 0.457233 | 5.1:-79147919 | MS.gene38130:CDS |
| GCTAATTGCAATTCTAATGA+TGG | 0.457837 | 5.1:-79147976 | MS.gene38130:CDS |
| GCACGAAGATTGTTTAAAAC+CGG | 0.468350 | 5.1:-79147745 | MS.gene38130:CDS |
| CCTAGTACTAGTGGTCTTGT+TGG | 0.468849 | 5.1:-79147592 | MS.gene38130:CDS |
| CAAAAGAAGAATAAGTTTGA+TGG | 0.471977 | 5.1:-79148327 | MS.gene38130:CDS |
| AGAATCCATAGTCAAGCTCT+AGG | 0.477759 | 5.1:-79148024 | MS.gene38130:CDS |
| CATGAGATCTCACCCTGAAA+GGG | 0.502988 | 5.1:-79147893 | MS.gene38130:CDS |
| CCAACAAGACCACTAGTACT+AGG | 0.509402 | 5.1:+79147592 | None:intergenic |
| TTGTTTAAAACCGGTAAAAG+AGG | 0.519380 | 5.1:-79147736 | MS.gene38130:CDS |
| ACATGAGATCTCACCCTGAA+AGG | 0.521597 | 5.1:-79147894 | MS.gene38130:CDS |
| TGATGGTGGTAGGATACATC+AGG | 0.528563 | 5.1:-79148310 | MS.gene38130:intron |
| TAGTCAAGCTCTAGGGAAAG+AGG | 0.530133 | 5.1:-79148016 | MS.gene38130:CDS |
| AGAATGACAATAACAAAAGA+AGG | 0.532053 | 5.1:-79147546 | MS.gene38130:CDS |
| AGGTGTGGAAAAGGATTAGG+TGG | 0.533754 | 5.1:-79148054 | MS.gene38130:CDS |
| CAATGAGGATTTGGATGATG+AGG | 0.536568 | 5.1:-79147635 | MS.gene38130:CDS |
| AGAATAAGTTTGATGGTGGT+AGG | 0.553715 | 5.1:-79148320 | MS.gene38130:CDS |
| GATCTCACCCTGAAAGGGAA+TGG | 0.563550 | 5.1:-79147888 | MS.gene38130:CDS |
| GAATCCATAGTCAAGCTCTA+GGG | 0.567403 | 5.1:-79148023 | MS.gene38130:CDS |
| TCACCCTGAAAGGGAATGGA+GGG | 0.580204 | 5.1:-79147884 | MS.gene38130:CDS |
| CTCACCCTGAAAGGGAATGG+AGG | 0.583418 | 5.1:-79147885 | MS.gene38130:CDS |
| TAACAAAAGAAGGAAGAAGC+TGG | 0.586820 | 5.1:-79147536 | MS.gene38130:CDS |
| GGTAAAAGAGGTCGTTCGAG+TGG | 0.603484 | 5.1:-79147724 | MS.gene38130:CDS |
| AGTCAAGCTCTAGGGAAAGA+GGG | 0.609576 | 5.1:-79148015 | MS.gene38130:CDS |
| TTTACACGACAACTTCAATG+AGG | 0.617038 | 5.1:-79147650 | MS.gene38130:CDS |
| AAGAAGAATAAGTTTGATGG+TGG | 0.622051 | 5.1:-79148324 | MS.gene38130:CDS |
| AATGTTGTACCTAGTACTAG+TGG | 0.625803 | 5.1:-79147601 | MS.gene38130:CDS |
| CACCCTGAAAGGGAATGGAG+GGG | 0.655548 | 5.1:-79147883 | MS.gene38130:CDS |
| AGAGGGTGAGTCGAAAGCAA+AGG | 0.661254 | 5.1:-79147998 | MS.gene38130:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGTAACAAAATTTTT+AGG | - | chr5.1:79147751-79147770 | MS.gene38130:CDS | 10.0% |
| !! | CAATATTATTATCCTTATTA+GGG | + | chr5.1:79147974-79147993 | None:intergenic | 15.0% |
| !! | TCAATATTATTATCCTTATT+AGG | + | chr5.1:79147975-79147994 | None:intergenic | 15.0% |
| !!! | TTTCTTTTAATAGAATTCTA+GGG | - | chr5.1:79147599-79147618 | MS.gene38130:CDS | 15.0% |
| !!! | TTTTCTTTTAATAGAATTCT+AGG | - | chr5.1:79147598-79147617 | MS.gene38130:CDS | 15.0% |
| !! | ATCAAAAGATAATGTCTTAT+TGG | - | chr5.1:79148337-79148356 | MS.gene38130:CDS | 20.0% |
| !! | TAAAGTTTATGAAAAACCAA+AGG | - | chr5.1:79147680-79147699 | MS.gene38130:CDS | 20.0% |
| ! | AGAATGACAATAACAAAAGA+AGG | - | chr5.1:79148279-79148298 | MS.gene38130:intron | 25.0% |
| ! | ATCATCAATAACCAATTCTT+TGG | + | chr5.1:79148045-79148064 | None:intergenic | 25.0% |
| ! | CAAAAGAAGAATAAGTTTGA+TGG | - | chr5.1:79147498-79147517 | MS.gene38130:CDS | 25.0% |
| !! | CATCAAACTTATTCTTCTTT+TGG | + | chr5.1:79147500-79147519 | None:intergenic | 25.0% |
| !! | TAAACTTTTCTTCTTCTCTA+TGG | + | chr5.1:79147640-79147659 | None:intergenic | 25.0% |
| !! | TGATGATGAATCAAAATCTT+GGG | - | chr5.1:79148058-79148077 | MS.gene38130:intron | 25.0% |
| !! | TGGTTTTTCATAAACTTTAC+AGG | + | chr5.1:79147679-79147698 | None:intergenic | 25.0% |
| !! | TTATCTTTTGATGTTGAACA+TGG | + | chr5.1:79148329-79148348 | None:intergenic | 25.0% |
| !! | TTATTCTTCTTTTGGTCATT+GGG | + | chr5.1:79147492-79147511 | None:intergenic | 25.0% |
| !!! | AAAATTTTTAGGTGTGGAAA+AGG | - | chr5.1:79147762-79147781 | MS.gene38130:CDS | 25.0% |
| !!! | AATAACCAATTCTTTGGAAT+TGG | + | chr5.1:79148039-79148058 | None:intergenic | 25.0% |
| !!! | TGTAACAAAATTTTTAGGTG+TGG | - | chr5.1:79147756-79147775 | MS.gene38130:CDS | 25.0% |
| !!! | TTGATGATGAATCAAAATCT+TGG | - | chr5.1:79148057-79148076 | MS.gene38130:intron | 25.0% |
| AAAGAACTTCCAATCCAATA+AGG | - | chr5.1:79147896-79147915 | MS.gene38130:CDS | 30.0% | |
| AAGAAGAATAAGTTTGATGG+TGG | - | chr5.1:79147501-79147520 | MS.gene38130:CDS | 30.0% | |
| GCTAATTGCAATTCTAATGA+TGG | - | chr5.1:79147849-79147868 | MS.gene38130:CDS | 30.0% | |
| GTTGTCGTGTAAATGTTTAT+CGG | + | chr5.1:79148168-79148187 | None:intergenic | 30.0% | |
| ! | CTTATTCTTCTTTTGGTCAT+TGG | + | chr5.1:79147493-79147512 | None:intergenic | 30.0% |
| ! | TTGTTTAAAACCGGTAAAAG+AGG | - | chr5.1:79148089-79148108 | MS.gene38130:intron | 30.0% |
| AAGGATCATCATCAAGATGA+AGG | - | chr5.1:79147699-79147718 | MS.gene38130:CDS | 35.0% | |
| AATGTTGTACCTAGTACTAG+TGG | - | chr5.1:79148224-79148243 | MS.gene38130:intron | 35.0% | |
| CAATCCAATAAGGCTTTACA+TGG | - | chr5.1:79147906-79147925 | MS.gene38130:CDS | 35.0% | |
| TAACAAAAGAAGGAAGAAGC+TGG | - | chr5.1:79148289-79148308 | MS.gene38130:intron | 35.0% | |
| TGCATCCAATTCCAAAGAAT+TGG | - | chr5.1:79148031-79148050 | MS.gene38130:CDS | 35.0% | |
| TTTACACGACAACTTCAATG+AGG | - | chr5.1:79148175-79148194 | MS.gene38130:CDS | 35.0% | |
| TTTAGGTGTGGAAAAGGATT+AGG | - | chr5.1:79147768-79147787 | MS.gene38130:CDS | 35.0% | |
| TTTGGATGATGAGGATAAGA+AGG | - | chr5.1:79148199-79148218 | MS.gene38130:CDS | 35.0% | |
| ! | CTTCTTCTCTATGGTTTTCA+TGG | + | chr5.1:79147631-79147650 | None:intergenic | 35.0% |
| ! | GCACGAAGATTGTTTAAAAC+CGG | - | chr5.1:79148080-79148099 | MS.gene38130:intron | 35.0% |
| !! | AGAATAAGTTTGATGGTGGT+AGG | - | chr5.1:79147505-79147524 | MS.gene38130:CDS | 35.0% |
| !! | CATGTAAAGCCTTATTGGAT+TGG | + | chr5.1:79147908-79147927 | None:intergenic | 35.0% |
| !! | TCATCTTGATGATGATCCTT+TGG | + | chr5.1:79147699-79147718 | None:intergenic | 35.0% |
| AGAATCCATAGTCAAGCTCT+AGG | - | chr5.1:79147801-79147820 | MS.gene38130:CDS | 40.0% | |
| CAATGAGGATTTGGATGATG+AGG | - | chr5.1:79148190-79148209 | MS.gene38130:CDS | 40.0% | |
| CGACAACTTCAATGAGGATT+TGG | - | chr5.1:79148181-79148200 | MS.gene38130:CDS | 40.0% | |
| GAATCCATAGTCAAGCTCTA+GGG | - | chr5.1:79147802-79147821 | MS.gene38130:CDS | 40.0% | |
| ! | GTGACCATGTAAAGCCTTAT+TGG | + | chr5.1:79147913-79147932 | None:intergenic | 40.0% |
| ACATGAGATCTCACCCTGAA+AGG | - | chr5.1:79147931-79147950 | MS.gene38130:CDS | 45.0% | |
| AGGTGTGGAAAAGGATTAGG+TGG | - | chr5.1:79147771-79147790 | MS.gene38130:CDS | 45.0% | |
| CATGAGATCTCACCCTGAAA+GGG | - | chr5.1:79147932-79147951 | MS.gene38130:CDS | 45.0% | |
| CCAACAAGACCACTAGTACT+AGG | + | chr5.1:79148236-79148255 | None:intergenic | 45.0% | |
| CTTTCCCTAGAGCTTGACTA+TGG | + | chr5.1:79147809-79147828 | None:intergenic | 45.0% | |
| ! | ACTCGAACGACCTCTTTTAC+CGG | + | chr5.1:79148102-79148121 | None:intergenic | 45.0% |
| ! | GAGGGGTATGAACCCTAATA+AGG | - | chr5.1:79147959-79147978 | MS.gene38130:CDS | 45.0% |
| !! | AGTCAAGCTCTAGGGAAAGA+GGG | - | chr5.1:79147810-79147829 | MS.gene38130:CDS | 45.0% |
| !! | CCTAGTACTAGTGGTCTTGT+TGG | - | chr5.1:79148233-79148252 | MS.gene38130:intron | 45.0% |
| !! | TAGTCAAGCTCTAGGGAAAG+AGG | - | chr5.1:79147809-79147828 | MS.gene38130:CDS | 45.0% |
| !! | TGATGGTGGTAGGATACATC+AGG | - | chr5.1:79147515-79147534 | MS.gene38130:CDS | 45.0% |
| AGAGGGTGAGTCGAAAGCAA+AGG | - | chr5.1:79147827-79147846 | MS.gene38130:CDS | 50.0% | |
| ATACCCCTCCATTCCCTTTC+AGG | + | chr5.1:79147948-79147967 | None:intergenic | 50.0% | |
| GATCTCACCCTGAAAGGGAA+TGG | - | chr5.1:79147937-79147956 | MS.gene38130:CDS | 50.0% | |
| GGTAAAAGAGGTCGTTCGAG+TGG | - | chr5.1:79148101-79148120 | MS.gene38130:intron | 50.0% | |
| TACCCCTCCATTCCCTTTCA+GGG | + | chr5.1:79147947-79147966 | None:intergenic | 50.0% | |
| TCACCCTGAAAGGGAATGGA+GGG | - | chr5.1:79147941-79147960 | MS.gene38130:CDS | 50.0% | |
| CACCCTGAAAGGGAATGGAG+GGG | - | chr5.1:79147942-79147961 | MS.gene38130:CDS | 55.0% | |
| CTCACCCTGAAAGGGAATGG+AGG | - | chr5.1:79147940-79147959 | MS.gene38130:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 79147483 | 79148364 | 79147483 | ID=MS.gene38130 |
| chr5.1 | mRNA | 79147483 | 79148364 | 79147483 | ID=MS.gene38130.t1;Parent=MS.gene38130 |
| chr5.1 | exon | 79148311 | 79148364 | 79148311 | ID=MS.gene38130.t1.exon1;Parent=MS.gene38130.t1 |
| chr5.1 | CDS | 79148311 | 79148364 | 79148311 | ID=cds.MS.gene38130.t1;Parent=MS.gene38130.t1 |
| chr5.1 | exon | 79148174 | 79148235 | 79148174 | ID=MS.gene38130.t1.exon2;Parent=MS.gene38130.t1 |
| chr5.1 | CDS | 79148174 | 79148235 | 79148174 | ID=cds.MS.gene38130.t1;Parent=MS.gene38130.t1 |
| chr5.1 | exon | 79147483 | 79148074 | 79147483 | ID=MS.gene38130.t1.exon3;Parent=MS.gene38130.t1 |
| chr5.1 | CDS | 79147483 | 79148074 | 79147483 | ID=cds.MS.gene38130.t1;Parent=MS.gene38130.t1 |
| Gene Sequence |
| Protein sequence |
