AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38194

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38194.t1 MTR_5g097500 80.707 311 27 2 1 278 1 311 0.0 503
MS.gene38194.t1 MTR_5g097490 49.695 328 110 4 1 273 1 328 6.00e-101 300
MS.gene38194.t1 MTR_4g021780 72.727 99 26 1 71 169 15 112 4.96e-49 158
MS.gene38194.t1 MTR_7g071120 33.061 245 118 5 73 271 159 403 2.06e-35 132
MS.gene38194.t1 MTR_4g013260 35.242 227 109 6 72 261 175 400 3.59e-35 132
MS.gene38194.t1 MTR_3g056100 29.412 289 155 3 23 264 39 325 8.86e-34 127
MS.gene38194.t1 MTR_5g094430 31.250 240 119 3 80 273 67 306 3.45e-33 125
MS.gene38194.t1 MTR_3g056100 29.412 289 155 3 23 264 183 469 4.24e-33 127
MS.gene38194.t1 MTR_5g094430 31.250 240 119 3 80 273 89 328 4.91e-33 125
MS.gene38194.t1 MTR_5g094430 31.250 240 119 3 80 273 201 440 7.37e-33 126
MS.gene38194.t1 MTR_3g056100 28.146 302 155 4 23 264 39 338 3.52e-32 123
MS.gene38194.t1 MTR_5g074400 30.894 246 111 3 80 266 244 489 1.52e-31 123
MS.gene38194.t1 MTR_3g056100 28.146 302 155 4 23 264 183 482 1.54e-31 123
MS.gene38194.t1 MTR_3g031220 30.932 236 114 5 80 266 199 434 4.23e-31 121
MS.gene38194.t1 MTR_4g082580 32.394 213 129 5 80 277 146 358 4.48e-30 116
MS.gene38194.t1 MTR_7g100510 28.421 285 136 7 27 257 151 421 3.19e-28 112
MS.gene38194.t1 MTR_3g009470 33.607 122 70 2 153 263 440 561 7.61e-18 83.6
MS.gene38194.t1 MTR_3g009470 50.847 59 29 0 79 137 280 338 2.69e-14 73.2
MS.gene38194.t1 MTR_7g080020 41.111 90 53 0 182 271 478 567 2.05e-17 82.4
MS.gene38194.t1 MTR_7g080020 47.945 73 35 1 79 148 280 352 3.55e-15 75.9
MS.gene38194.t1 MTR_5g042920 39.080 87 53 0 182 268 305 391 3.52e-17 81.3
MS.gene38194.t1 MTR_5g042920 50.000 60 28 1 80 137 152 211 2.54e-12 67.0
MS.gene38194.t1 MTR_5g042920 36.957 92 55 2 187 276 139 229 5.09e-11 63.2
MS.gene38194.t1 MTR_7g080000 45.333 75 41 0 185 259 420 494 3.71e-17 81.6
MS.gene38194.t1 MTR_7g080000 46.774 62 33 0 79 140 234 295 1.36e-13 70.9
MS.gene38194.t1 MTR_7g011820 37.931 87 54 0 182 268 395 481 1.25e-16 79.7
MS.gene38194.t1 MTR_7g011820 50.847 59 29 0 80 138 229 287 4.59e-14 72.4
MS.gene38194.t1 MTR_3g031220 29.121 182 80 5 80 212 199 380 4.30e-16 77.8
MS.gene38194.t1 MTR_3g009470 50.847 59 29 0 79 137 280 338 3.48e-14 72.4
MS.gene38194.t1 MTR_8g096730 35.870 92 51 2 186 271 59 148 4.51e-14 68.6
MS.gene38194.t1 MTR_1g015140 44.928 69 38 0 185 253 90 158 4.59e-14 68.9
MS.gene38194.t1 MTR_1g077780 35.366 82 53 0 182 263 47 128 7.38e-14 67.8
MS.gene38194.t1 MTR_3g104750 43.478 69 39 0 185 253 92 160 7.84e-14 68.2
MS.gene38194.t1 MTR_8g092010 40.541 74 44 0 185 258 96 169 9.10e-14 68.6
MS.gene38194.t1 MTR_4g130900 42.667 75 43 0 185 259 347 421 1.17e-13 70.9
MS.gene38194.t1 MTR_7g079010 36.264 91 58 0 163 253 107 197 2.17e-13 68.2
MS.gene38194.t1 MTR_5g074200 35.200 125 73 1 17 141 166 282 2.46e-13 69.3
MS.gene38194.t1 MTR_7g109800 40.909 88 49 1 182 266 130 217 6.72e-13 67.8
MS.gene38194.t1 MTR_7g028415 43.243 74 40 1 186 259 82 153 1.21e-12 65.1
MS.gene38194.t1 MTR_7g028710 43.243 74 40 1 186 259 82 153 1.21e-12 65.1
MS.gene38194.t1 MTR_4g094588 45.455 77 41 1 185 260 166 242 1.62e-12 67.0
MS.gene38194.t1 MTR_8g007270 41.892 74 43 0 182 255 139 212 1.96e-12 65.5
MS.gene38194.t1 MTR_2g105060 36.905 84 52 1 174 256 71 154 3.01e-12 65.5
MS.gene38194.t1 MTR_2g105060 42.254 71 39 2 69 137 84 154 6.23e-11 61.6
MS.gene38194.t1 MTR_2g105060 36.905 84 52 1 174 256 146 229 4.59e-12 65.5
MS.gene38194.t1 MTR_2g033820 40.260 77 46 0 182 258 188 264 5.03e-12 65.9
MS.gene38194.t1 MTR_3g106060 44.444 72 37 2 185 253 124 195 7.63e-12 64.7
MS.gene38194.t1 MTR_3g095040 40.278 72 43 0 185 256 106 177 8.71e-12 63.5
MS.gene38194.t1 MTR_5g043880 38.889 90 52 1 185 271 112 201 1.70e-11 63.5
MS.gene38194.t1 MTR_1g013760 47.887 71 35 2 185 253 127 197 1.79e-11 63.5
MS.gene38194.t1 MTR_1g086790 40.741 81 47 1 182 261 143 223 1.93e-11 63.9
MS.gene38194.t1 MTR_7g110720 37.975 79 47 2 187 263 179 257 2.35e-11 63.9
MS.gene38194.t1 MTR_7g110720 37.975 79 47 2 187 263 206 284 2.57e-11 63.9
MS.gene38194.t1 MTR_4g007060 38.636 88 45 3 188 266 119 206 3.61e-11 62.8
MS.gene38194.t1 MTR_3g095080 40.541 74 44 0 182 255 129 202 3.78e-11 62.0
MS.gene38194.t1 MTR_4g007060 38.636 88 45 3 188 266 153 240 3.85e-11 62.8
MS.gene38194.t1 MTR_3g053330 25.882 170 110 4 108 276 177 331 3.88e-11 63.5
MS.gene38194.t1 MTR_3g095080 40.541 74 44 0 182 255 153 226 5.52e-11 61.6
MS.gene38194.t1 MTR_5g091390 41.558 77 44 1 185 260 145 221 6.89e-11 62.0
MS.gene38194.t1 MTR_4g049790 37.333 75 46 1 185 258 300 374 7.43e-11 62.8
MS.gene38194.t1 MTR_1g099600 35.106 94 55 2 191 278 179 272 8.73e-11 61.6
MS.gene38194.t1 MTR_1g078260 34.884 86 55 1 185 269 235 320 9.41e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38194.t1 AT2G04880 29.607 331 166 5 1 277 46 363 2.12e-40 145
MS.gene38194.t1 AT5G07100 36.757 185 106 4 80 259 25 203 5.04e-33 120
MS.gene38194.t1 AT5G07100 44.118 68 38 0 191 258 18 85 1.07e-12 66.2
MS.gene38194.t1 AT5G07100 36.757 185 106 4 80 259 25 203 5.04e-33 120
MS.gene38194.t1 AT5G07100 44.118 68 38 0 191 258 18 85 1.07e-12 66.2
MS.gene38194.t1 AT5G07100 36.757 185 106 4 80 259 25 203 5.04e-33 120
MS.gene38194.t1 AT5G07100 44.118 68 38 0 191 258 18 85 1.07e-12 66.2
MS.gene38194.t1 AT5G07100 36.757 185 106 4 80 259 25 203 5.04e-33 120
MS.gene38194.t1 AT5G07100 44.118 68 38 0 191 258 18 85 1.07e-12 66.2
MS.gene38194.t1 AT5G07100 36.757 185 106 4 80 259 118 296 3.41e-32 120
MS.gene38194.t1 AT5G07100 44.118 68 38 0 191 258 111 178 2.65e-12 66.2
MS.gene38194.t1 AT3G01080 34.343 198 109 3 80 256 168 365 1.09e-31 121
MS.gene38194.t1 AT3G01080 34.343 198 109 3 80 256 206 403 1.56e-31 122
MS.gene38194.t1 AT2G03340 31.933 238 115 4 80 270 251 488 7.15e-31 120
MS.gene38194.t1 AT2G03340 41.667 84 48 1 188 271 241 323 2.88e-14 72.8
MS.gene38194.t1 AT2G30250 32.287 223 107 4 80 258 167 389 4.74e-30 117
MS.gene38194.t1 AT4G30935 26.882 279 150 6 27 259 123 393 1.32e-23 100
MS.gene38194.t1 AT2G04880 33.784 148 88 2 140 277 240 387 1.13e-20 91.7
MS.gene38194.t1 AT2G04880 34.965 143 80 2 1 143 46 175 3.20e-18 84.3
MS.gene38194.t1 AT2G04880 42.647 68 38 1 194 261 108 174 2.68e-12 67.0
MS.gene38194.t1 AT5G56270 44.872 78 43 0 182 259 472 549 2.89e-18 85.1
MS.gene38194.t1 AT5G56270 45.763 59 32 0 79 137 273 331 1.85e-11 64.7
MS.gene38194.t1 AT1G13960 42.667 75 43 0 182 256 241 315 9.07e-17 79.7
MS.gene38194.t1 AT1G13960 45.205 73 39 1 184 256 63 134 5.13e-14 71.6
MS.gene38194.t1 AT1G13960 50.820 61 30 0 80 140 77 137 2.92e-13 69.3
MS.gene38194.t1 AT1G13960 42.667 75 43 0 182 256 394 468 1.05e-16 80.1
MS.gene38194.t1 AT1G13960 45.205 73 39 1 184 256 216 287 4.55e-14 72.4
MS.gene38194.t1 AT1G13960 50.820 61 30 0 80 140 230 290 1.73e-13 70.5
MS.gene38194.t1 AT2G38470 43.038 79 45 0 182 260 347 425 1.69e-16 79.3
MS.gene38194.t1 AT2G38470 48.333 60 31 0 79 138 184 243 1.09e-12 68.2
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 190 267 8.37e-16 77.0
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 18 94 1.83e-15 75.9
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 190 267 8.37e-16 77.0
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 18 94 1.83e-15 75.9
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 190 267 8.37e-16 77.0
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 18 94 1.83e-15 75.9
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 190 267 8.37e-16 77.0
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 18 94 1.83e-15 75.9
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 154 230 1.07e-15 77.0
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 326 403 1.18e-15 77.0
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 294 371 1.19e-15 77.0
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 122 198 1.55e-15 76.6
MS.gene38194.t1 AT4G26640 44.156 77 42 1 63 138 194 270 1.22e-15 77.0
MS.gene38194.t1 AT4G26640 43.590 78 44 0 182 259 366 443 1.30e-15 77.0
MS.gene38194.t1 AT4G26440 42.683 82 47 0 182 263 357 438 1.68e-15 76.6
MS.gene38194.t1 AT4G26440 48.276 58 30 0 80 137 179 236 4.25e-12 66.6
MS.gene38194.t1 AT5G13080 44.595 74 39 1 186 259 56 127 2.24e-14 69.3
MS.gene38194.t1 AT3G01080 52.542 59 28 0 80 138 206 264 2.42e-14 72.4
MS.gene38194.t1 AT5G64810 40.845 71 42 0 185 255 98 168 1.24e-13 68.6
MS.gene38194.t1 AT5G26170 43.478 69 39 0 185 253 101 169 2.53e-13 67.0
MS.gene38194.t1 AT2G25000 39.080 87 49 2 184 266 90 176 4.76e-13 67.4
MS.gene38194.t1 AT2G25000 39.080 87 49 2 184 266 90 176 4.76e-13 67.4
MS.gene38194.t1 AT2G25000 39.080 87 49 2 184 266 112 198 5.96e-13 67.4
MS.gene38194.t1 AT2G25000 39.080 87 49 2 184 266 133 219 7.56e-13 67.4
MS.gene38194.t1 AT1G55600 43.056 72 41 0 185 256 295 366 1.37e-12 67.8
MS.gene38194.t1 AT2G21900 38.158 76 45 1 183 256 93 168 1.52e-12 65.5
MS.gene38194.t1 AT5G49520 42.500 80 45 1 182 260 206 285 2.29e-12 67.0
MS.gene38194.t1 AT2G47260 40.260 77 46 0 182 258 159 235 2.51e-12 66.6
MS.gene38194.t1 AT2G37260 50.000 58 29 0 80 137 166 223 3.73e-12 66.2
MS.gene38194.t1 AT2G44745 41.892 74 43 0 182 255 130 203 4.21e-12 64.7
MS.gene38194.t1 AT2G44745 43.662 71 39 1 77 146 143 213 4.02e-11 61.6
MS.gene38194.t1 AT5G41570 43.478 69 39 0 185 253 86 154 4.80e-12 63.5
MS.gene38194.t1 AT2G37260 50.000 58 29 0 80 137 86 143 7.32e-12 65.1
MS.gene38194.t1 AT2G37260 50.000 58 29 0 80 137 84 141 7.89e-12 65.1
MS.gene38194.t1 AT4G23550 38.667 75 45 1 186 259 129 203 8.25e-12 64.7
MS.gene38194.t1 AT3G62340 46.774 62 33 0 196 257 117 178 8.70e-12 64.3
MS.gene38194.t1 AT1G64000 43.478 69 39 0 185 253 102 170 1.04e-11 63.2
MS.gene38194.t1 AT1G64000 45.000 60 32 1 77 135 112 171 4.63e-11 61.2
MS.gene38194.t1 AT4G01720 45.070 71 38 1 187 256 229 299 1.25e-11 65.1
MS.gene38194.t1 AT1G69310 42.857 70 40 0 185 254 135 204 1.50e-11 63.9
MS.gene38194.t1 AT1G69310 42.857 70 40 0 185 254 135 204 1.50e-11 63.9
MS.gene38194.t1 AT1G69310 42.857 70 40 0 185 254 135 204 1.50e-11 63.9
MS.gene38194.t1 AT3G01970 42.647 68 39 0 186 253 54 121 2.38e-11 60.8
MS.gene38194.t1 AT2G46130 41.791 67 39 0 187 253 20 86 3.56e-11 59.3
MS.gene38194.t1 AT4G31800 38.667 75 45 1 183 256 97 171 3.68e-11 62.4
MS.gene38194.t1 AT1G80840 30.345 145 87 4 124 256 64 206 4.03e-11 62.8
MS.gene38194.t1 AT4G31800 38.667 75 45 1 183 256 162 236 4.96e-11 62.4
MS.gene38194.t1 AT5G15130 43.077 65 36 1 195 258 225 289 7.01e-11 62.8
MS.gene38194.t1 AT1G29860 40.000 75 45 0 182 256 121 195 7.02e-11 62.0
MS.gene38194.t1 AT4G18170 41.975 81 45 1 182 262 157 235 7.33e-11 62.0
MS.gene38194.t1 AT5G15130 43.077 65 36 1 195 258 183 247 8.56e-11 62.4

Find 49 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATAATGCGTATTAA+TGG 0.253581 5.3:-78820320 MS.gene38194:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5.3:-78820402 MS.gene38194:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5.3:+78820388 None:intergenic
ACTTCCACACCGGTCAAATA+TGG 0.326583 5.3:+78820887 None:intergenic
AATTCATGTGAAGGAGTATT+AGG 0.352642 5.3:+78821205 None:intergenic
TGTTCTTCCTGTTGTTGAAC+AGG 0.360275 5.3:-78820921 MS.gene38194:CDS
ACAATGCTTGATTGAGGATT+AGG 0.367320 5.3:+78820959 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5.3:+78820416 None:intergenic
ACTAGAGTTGGCGTGTTAAC+TGG 0.387351 5.3:-78820761 MS.gene38194:CDS
TGAATGTGTGCATTTGTAAT+AGG 0.403229 5.3:+78821063 None:intergenic
TTGTAAATGATGCGTATTGC+TGG 0.413208 5.3:-78820697 MS.gene38194:CDS
AAATTTCATTGGTCAAATGA+TGG 0.419177 5.3:-78821022 MS.gene38194:CDS
GCGTATTGCTGGCGCAAATA+CGG 0.423416 5.3:-78820686 MS.gene38194:CDS
TCGCTCAACCATACGTGAAA+TGG 0.426220 5.3:-78821155 MS.gene38194:CDS
TACTTTCGATGCGCCTATCC+AGG 0.436737 5.3:-78820470 MS.gene38194:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5.3:-78820401 MS.gene38194:CDS
ATCATGAACCACCGATTAGT+AGG 0.455805 5.3:-78820370 MS.gene38194:CDS
TGACGAACACCCCTACTAAT+CGG 0.461221 5.3:+78820359 None:intergenic
CGTGAAATGGTAAGAAAAGA+TGG 0.461858 5.3:-78821142 MS.gene38194:CDS
ACAGGAAGAACATGATCAAC+TGG 0.462404 5.3:+78820932 None:intergenic
TGTGGTCCCTGTTCAACAAC+AGG 0.490907 5.3:+78820914 None:intergenic
TTTCACTGTGCATCCTGGAT+AGG 0.494274 5.3:+78820457 None:intergenic
TTGACCGGTGTGGAAGTTCA+AGG 0.496310 5.3:-78820881 MS.gene38194:intron
AATTTCATTGGTCAAATGAT+GGG 0.502281 5.3:-78821021 MS.gene38194:CDS
GTTCTTCCTGTTGTTGAACA+GGG 0.504635 5.3:-78820920 MS.gene38194:CDS
AGAAACTAATGATGTAGCTA+TGG 0.532105 5.3:-78821269 MS.gene38194:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5.3:+78820417 None:intergenic
TAAGAAAAGATGGCTATAAG+TGG 0.538492 5.3:-78821132 MS.gene38194:CDS
TCATGAACCACCGATTAGTA+GGG 0.538550 5.3:-78820369 MS.gene38194:CDS
AATACTGATGCTACTAGAGT+TGG 0.539414 5.3:-78820773 MS.gene38194:CDS
CCACATCAACCATATTTGAC+CGG 0.557825 5.3:-78820896 MS.gene38194:CDS
ACTCGATGTTGTACTTCAGA+AGG 0.565037 5.3:+78821322 None:intergenic
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5.3:-78821257 MS.gene38194:CDS
TATTGATTAGATTGTATCAG+AGG 0.571636 5.3:+78821181 None:intergenic
ATCAGAGGTAATTCATGTGA+AGG 0.594434 5.3:+78821196 None:intergenic
GATGTAACTGAAATATTCGA+CGG 0.605617 5.3:+78820997 None:intergenic
CCGGTCAAATATGGTTGATG+TGG 0.605641 5.3:+78820896 None:intergenic
GGTGGTACAATGCTTGATTG+AGG 0.614087 5.3:+78820953 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5.3:+78820418 None:intergenic
ACATCGAGTGAATCCTCAGA+GGG 0.633099 5.3:-78821308 MS.gene38194:CDS
GACAATGTGATCTCCCTCTG+AGG 0.634875 5.3:+78821295 None:intergenic
CACATATGCAACACTCGCAG+AGG 0.656505 5.3:-78821228 MS.gene38194:CDS
ATCACCTTGAACTTCCACAC+CGG 0.673408 5.3:+78820877 None:intergenic
CACTTGAAGTCTGAACAACA+AGG 0.680480 5.3:+78820729 None:intergenic
CATGAACCACCGATTAGTAG+GGG 0.682009 5.3:-78820368 MS.gene38194:CDS
TCAACCATATTTGACCGGTG+TGG 0.682556 5.3:-78820891 MS.gene38194:CDS
GGAAGAACATGATCAACTGG+TGG 0.702342 5.3:+78820935 None:intergenic
CGAACACCCCTACTAATCGG+TGG 0.705915 5.3:+78820362 None:intergenic
AACATCGAGTGAATCCTCAG+AGG 0.727570 5.3:-78821309 MS.gene38194:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG + chr5.3:78820862-78820881 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG - chr5.3:78821019-78821038 MS.gene38194:CDS 20.0%
! AAATTTCATTGGTCAAATGA+TGG - chr5.3:78820649-78820668 MS.gene38194:intron 25.0%
! AATGTAACAACTACCTATAA+GGG - chr5.3:78821270-78821289 MS.gene38194:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG - chr5.3:78820650-78820669 MS.gene38194:intron 25.0%
! ACTCAAATAATGCGTATTAA+TGG - chr5.3:78821351-78821370 MS.gene38194:CDS 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG + chr5.3:78821050-78821069 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG - chr5.3:78820638-78820657 MS.gene38194:intron 25.0%
! TAATGTAACAACTACCTATA+AGG - chr5.3:78821269-78821288 MS.gene38194:CDS 25.0%
! TTACATTAGTCACATTTAGA+GGG + chr5.3:78821257-78821276 None:intergenic 25.0%
!! TATTGATTAGATTGTATCAG+AGG + chr5.3:78820493-78820512 None:intergenic 25.0%
AGAAACTAATGATGTAGCTA+TGG - chr5.3:78820402-78820421 MS.gene38194:CDS 30.0%
GATGTAACTGAAATATTCGA+CGG + chr5.3:78820677-78820696 None:intergenic 30.0%
GGCTATAAGTGGAAAAAATA+TGG - chr5.3:78820550-78820569 MS.gene38194:intron 30.0%
GTTACATTAGTCACATTTAG+AGG + chr5.3:78821258-78821277 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG - chr5.3:78820539-78820558 MS.gene38194:intron 30.0%
TACATTAGTCACATTTAGAG+GGG + chr5.3:78821256-78821275 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG - chr5.3:78821003-78821022 MS.gene38194:CDS 30.0%
TGAATGTGTGCATTTGTAAT+AGG + chr5.3:78820611-78820630 None:intergenic 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG + chr5.3:78821188-78821207 None:intergenic 30.0%
! TTTTCTTACCATTTCACGTA+TGG + chr5.3:78820527-78820546 None:intergenic 30.0%
!! AATTCATGTGAAGGAGTATT+AGG + chr5.3:78820469-78820488 None:intergenic 30.0%
AATACTGATGCTACTAGAGT+TGG - chr5.3:78820898-78820917 MS.gene38194:CDS 35.0%
ACAATGCTTGATTGAGGATT+AGG + chr5.3:78820715-78820734 None:intergenic 35.0%
ATCAGAGGTAATTCATGTGA+AGG + chr5.3:78820478-78820497 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG + chr5.3:78821286-78821305 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG - chr5.3:78820529-78820548 MS.gene38194:intron 35.0%
TTGTAAATGATGCGTATTGC+TGG - chr5.3:78820974-78820993 MS.gene38194:CDS 35.0%
! AGTAATTTCTGGAAGATTGG+AGG + chr5.3:78821185-78821204 None:intergenic 35.0%
! GTAATTTCTGGAAGATTGGA+GGG + chr5.3:78821184-78821203 None:intergenic 35.0%
ACAGGAAGAACATGATCAAC+TGG + chr5.3:78820742-78820761 None:intergenic 40.0%
ACTCGATGTTGTACTTCAGA+AGG + chr5.3:78820352-78820371 None:intergenic 40.0%
CACTTGAAGTCTGAACAACA+AGG + chr5.3:78820945-78820964 None:intergenic 40.0%
CCACATCAACCATATTTGAC+CGG - chr5.3:78820775-78820794 MS.gene38194:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG - chr5.3:78820996-78821015 MS.gene38194:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG - chr5.3:78820751-78820770 MS.gene38194:CDS 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG - chr5.3:78820750-78820769 MS.gene38194:CDS 40.0%
! ATCATGAACCACCGATTAGT+AGG - chr5.3:78821301-78821320 MS.gene38194:CDS 40.0%
! TCATGAACCACCGATTAGTA+GGG - chr5.3:78821302-78821321 MS.gene38194:CDS 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG + chr5.3:78821222-78821241 None:intergenic 40.0%
AACATCGAGTGAATCCTCAG+AGG - chr5.3:78820362-78820381 MS.gene38194:CDS 45.0%
ACATCGAGTGAATCCTCAGA+GGG - chr5.3:78820363-78820382 MS.gene38194:CDS 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG - chr5.3:78820910-78820929 MS.gene38194:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG + chr5.3:78820787-78820806 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG + chr5.3:78820797-78820816 None:intergenic 45.0%
GGAAGAACATGATCAACTGG+TGG + chr5.3:78820739-78820758 None:intergenic 45.0%
GGCGCATCGAAAGTAATTTC+TGG + chr5.3:78821196-78821215 None:intergenic 45.0%
GGTGGTACAATGCTTGATTG+AGG + chr5.3:78820721-78820740 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG - chr5.3:78820780-78820799 MS.gene38194:CDS 45.0%
TCGCTCAACCATACGTGAAA+TGG - chr5.3:78820516-78820535 MS.gene38194:intron 45.0%
TGACGAACACCCCTACTAAT+CGG + chr5.3:78821315-78821334 None:intergenic 45.0%
TTTCACTGTGCATCCTGGAT+AGG + chr5.3:78821217-78821236 None:intergenic 45.0%
! CATGAACCACCGATTAGTAG+GGG - chr5.3:78821303-78821322 MS.gene38194:CDS 45.0%
! CCGGTCAAATATGGTTGATG+TGG + chr5.3:78820778-78820797 None:intergenic 45.0%
! TGTAGCTATGGCTTGTGACT+TGG - chr5.3:78820414-78820433 MS.gene38194:CDS 45.0%
CACATATGCAACACTCGCAG+AGG - chr5.3:78820443-78820462 MS.gene38194:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG + chr5.3:78820379-78820398 None:intergenic 50.0%
GCGTATTGCTGGCGCAAATA+CGG - chr5.3:78820985-78821004 MS.gene38194:CDS 50.0%
TACTTTCGATGCGCCTATCC+AGG - chr5.3:78821201-78821220 MS.gene38194:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG + chr5.3:78820760-78820779 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG - chr5.3:78820790-78820809 MS.gene38194:intron 50.0%
CGAACACCCCTACTAATCGG+TGG + chr5.3:78821312-78821331 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 78820310 78821383 78820310 ID=MS.gene38194
chr5.3 mRNA 78820310 78821383 78820310 ID=MS.gene38194.t1;Parent=MS.gene38194
chr5.3 exon 78820882 78821383 78820882 ID=MS.gene38194.t1.exon1;Parent=MS.gene38194.t1
chr5.3 CDS 78820882 78821383 78820882 ID=cds.MS.gene38194.t1;Parent=MS.gene38194.t1
chr5.3 exon 78820653 78820800 78820653 ID=MS.gene38194.t1.exon2;Parent=MS.gene38194.t1
chr5.3 CDS 78820653 78820800 78820653 ID=cds.MS.gene38194.t1;Parent=MS.gene38194.t1
chr5.3 exon 78820310 78820496 78820310 ID=MS.gene38194.t1.exon3;Parent=MS.gene38194.t1
chr5.3 CDS 78820310 78820496 78820310 ID=cds.MS.gene38194.t1;Parent=MS.gene38194.t1
Gene Sequence

>MS.gene38194

ATGGTTTTGTCACTACAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACAACATCGAGTGAATCCTCAGAGGGAGATCACATTGTCACGAGAAACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATATGCAACACTCGCAGAGGCCTAATACTCCTTCACATGAATTACCTCTGATACAATCTAATCAATACACTCGCTCAACCATACGTGAAATGGTAAGAAAAGATGGCTATAAGTGGAAAAAATATGGTGAGAAAAACATTAAGAAGAATGAACATAAAAGAGCCTATTACAAATGCACACATTCAGACTGTCGTGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACCGTCGAATATTTCAGTTACATCAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACCAGTTGATCATGTTCTTCCTGTTGTTGAACAGGGACCACATCAACCATATTTGACCGGTGTGGAAGTTCAAGGTGATAAGTACTCTGTTAGTATTTTAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATACTGATGCTACTAGAGTTGGCGTGTTAACTGGTGAACTTCACCTTGTTGTTCAGACTTCAAGTGCAAATGAAGTTGTAAATGATGCGTATTGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGGTAAATCCTAGCTTTTATTTTGAACTCATACATTGTAGATCATTTCAATATGCATTTTTTTATTGCTTTTTCTCTATGTGCTTTAAGCTTTTGCAAATACACAAATTCATGAAATGATTTTATCTAATTGATTGCATTTTCCCTCCAATCTTCCAGAAATTACTTTCGATGCGCCTATCCAGGATGCACAGTGAAAAAGTATGTTCAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGATTAGTAGGGGTGTTCGTCATGACTCTGTTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>MS.gene38194.t1

MVLSLQNVDHNDSPSEVQHRVNPQREITLSRETNDVAMACDLVLSHMQHSQRPNTPSHELPLIQSNQYTRSTIREMVRKDGYKWKKYGEKNIKKNEHKRAYYKCTHSDCRAKKKFHWSNDGTVEYFSYINQHNHPNPQSSIVPPVDHVLPVVEQGPHQPYLTGVEVQDNNTDATRVGVLTGELHLVVQTSSANEVVNDAYCWRKYGRKMVNGKTIQRNYFRCAYPGCTVKKYVQKSPLNVTNVTTTYKGQHDHEPPISRGVRHDSVTNTQIMRINGEP