AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38196

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38196.t1 MTR_5g097480 98.897 544 6 0 1 544 1 544 0.0 1129
MS.gene38196.t1 MTR_3g056110 57.713 551 217 5 1 544 1 542 0.0 650
MS.gene38196.t1 MTR_5g094450 56.757 555 206 8 1 544 1 532 0.0 618
MS.gene38196.t1 MTR_4g133660 57.169 551 219 8 1 543 1 542 0.0 606
MS.gene38196.t1 MTR_2g082090 63.824 387 137 1 157 543 195 578 0.0 526
MS.gene38196.t1 MTR_2g026250 46.780 590 249 8 12 544 15 596 3.54e-178 515
MS.gene38196.t1 MTR_1g029420 56.216 370 162 0 175 544 223 592 2.65e-159 467
MS.gene38196.t1 MTR_3g089055 54.278 374 171 0 171 544 192 565 6.01e-151 444
MS.gene38196.t1 MTR_3g089055 54.278 374 171 0 171 544 197 570 7.05e-151 444
MS.gene38196.t1 MTR_7g027190 40.204 393 205 5 170 544 293 673 3.33e-87 283
MS.gene38196.t1 MTR_3g065980 36.923 390 209 7 174 544 169 540 2.20e-73 243
MS.gene38196.t1 MTR_7g062120 34.048 373 245 1 173 544 368 740 3.65e-69 236
MS.gene38196.t1 MTR_4g064160 32.796 372 249 1 173 543 312 683 2.73e-65 225
MS.gene38196.t1 MTR_8g442410 33.594 384 234 7 173 544 151 525 7.70e-65 220
MS.gene38196.t1 MTR_4g064150 32.447 376 249 3 173 543 357 732 1.25e-64 224
MS.gene38196.t1 MTR_2g097390 29.352 494 309 10 74 543 163 640 4.02e-64 221
MS.gene38196.t1 MTR_4g064200 33.245 376 246 4 170 543 277 649 4.77e-64 221
MS.gene38196.t1 MTR_7g057230 33.494 415 255 7 140 543 252 656 5.49e-63 218
MS.gene38196.t1 MTR_2g097310 28.484 488 316 9 74 543 165 637 2.22e-62 216
MS.gene38196.t1 MTR_3g022830 31.265 419 228 7 178 544 26 436 1.30e-60 206
MS.gene38196.t1 MTR_6g047750 35.354 396 204 13 175 544 293 662 1.62e-60 211
MS.gene38196.t1 MTR_4g076140 30.787 432 231 7 174 544 45 469 3.53e-60 206
MS.gene38196.t1 MTR_4g064120 30.144 418 281 4 129 541 212 623 4.91e-60 209
MS.gene38196.t1 MTR_2g097350 31.200 375 249 4 173 543 270 639 6.16e-60 209
MS.gene38196.t1 MTR_4g104020 32.292 384 238 6 174 544 146 520 1.36e-59 206
MS.gene38196.t1 MTR_2g097410 31.117 376 252 4 173 543 367 740 1.93e-59 210
MS.gene38196.t1 MTR_2g097467 31.095 402 270 4 143 543 258 653 3.00e-59 208
MS.gene38196.t1 MTR_4g064180 30.456 417 279 4 129 540 212 622 3.12e-59 207
MS.gene38196.t1 MTR_4g097080 30.890 382 243 7 175 544 131 503 4.47e-59 204
MS.gene38196.t1 MTR_5g009080 29.792 433 235 7 174 544 53 478 5.56e-58 201
MS.gene38196.t1 MTR_2g097473 32.976 373 244 5 173 543 285 653 6.25e-58 204
MS.gene38196.t1 MTR_7g074650 34.511 368 220 7 181 544 442 792 1.46e-57 206
MS.gene38196.t1 MTR_2g097463 31.720 372 250 3 173 543 286 654 1.04e-55 198
MS.gene38196.t1 MTR_4g102790 32.461 382 237 10 174 543 289 661 8.52e-52 188
MS.gene38196.t1 MTR_5g015490 29.592 392 253 8 169 544 106 490 9.10e-52 184
MS.gene38196.t1 MTR_2g097380 27.406 478 295 11 74 543 127 560 6.41e-50 181
MS.gene38196.t1 MTR_1g096030 31.567 453 251 16 127 544 31 459 1.62e-49 177
MS.gene38196.t1 MTR_4g077760 32.979 376 237 8 174 544 190 555 6.74e-47 172
MS.gene38196.t1 MTR_4g076020 33.062 369 215 9 181 544 88 429 2.20e-46 168
MS.gene38196.t1 MTR_5g058860 31.008 387 244 10 174 543 116 496 6.94e-44 163
MS.gene38196.t1 MTR_3g072710 29.426 401 250 10 167 543 108 499 4.41e-43 160
MS.gene38196.t1 MTR_2g089100 27.295 403 251 8 174 543 49 442 1.25e-41 155
MS.gene38196.t1 MTR_2g099110 28.974 390 243 9 177 544 74 451 3.33e-40 152
MS.gene38196.t1 MTR_1g069725 30.053 376 240 10 173 543 105 462 7.46e-40 151
MS.gene38196.t1 MTR_2g034250 25.974 385 257 10 178 544 211 585 1.84e-35 140
MS.gene38196.t1 MTR_4g095500 26.794 418 252 9 174 543 56 467 2.37e-34 135
MS.gene38196.t1 MTR_5g015950 25.806 434 238 12 174 543 32 445 3.27e-34 135
MS.gene38196.t1 MTR_3g053270 25.806 434 238 12 174 543 32 445 3.27e-34 135
MS.gene38196.t1 MTR_2g034260 25.882 340 229 8 178 503 211 541 2.01e-30 125
MS.gene38196.t1 MTR_2g034280 24.074 378 270 7 178 544 204 575 2.35e-30 125
MS.gene38196.t1 MTR_4g026485 24.595 370 261 8 178 544 270 624 2.31e-29 122
MS.gene38196.t1 MTR_0092s0100 23.662 355 254 7 193 544 388 728 3.45e-26 113
MS.gene38196.t1 MTR_0092s0100 23.662 355 254 7 193 544 391 731 3.63e-26 113
MS.gene38196.t1 MTR_7g109580 23.770 366 248 10 190 544 209 554 6.77e-26 112
MS.gene38196.t1 MTR_8g093070 28.186 408 249 19 154 543 114 495 1.05e-25 110
MS.gene38196.t1 MTR_1g086970 23.143 350 246 10 208 544 139 478 1.79e-20 94.7
MS.gene38196.t1 MTR_8g020840 25.185 405 243 16 175 544 169 548 5.29e-19 90.9
MS.gene38196.t1 MTR_3g022580 39.286 112 64 2 435 544 74 183 3.51e-18 83.2
MS.gene38196.t1 MTR_3g022005 43.158 95 50 2 452 544 33 125 1.54e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38196.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene38196.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene38196.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene38196.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene38196.t1 AT1G50600 61.741 494 181 4 56 544 111 601 0.0 617
MS.gene38196.t1 AT1G50600 61.741 494 181 4 56 544 107 597 0.0 616
MS.gene38196.t1 AT1G50600 61.741 494 181 4 56 544 36 526 0.0 615
MS.gene38196.t1 AT2G04890 68.841 414 122 1 131 544 7 413 0.0 597
MS.gene38196.t1 AT4G17230 52.834 547 233 8 1 544 1 525 0.0 560
MS.gene38196.t1 AT4G17230 52.834 547 233 8 1 544 1 525 0.0 560
MS.gene38196.t1 AT1G21450 48.423 444 212 4 118 544 150 593 7.93e-150 442
MS.gene38196.t1 AT1G66350 35.678 398 221 11 158 544 133 506 1.68e-70 235
MS.gene38196.t1 AT3G03450 36.800 375 224 7 174 544 180 545 1.72e-70 236
MS.gene38196.t1 AT1G14920 36.061 391 225 9 163 544 155 529 3.23e-70 234
MS.gene38196.t1 AT2G01570 36.869 396 221 12 160 544 204 581 1.68e-68 231
MS.gene38196.t1 AT1G07530 33.333 375 248 2 171 543 390 764 3.23e-68 234
MS.gene38196.t1 AT5G52510 34.286 385 228 7 175 544 266 640 2.62e-66 227
MS.gene38196.t1 AT5G17490 34.933 375 225 9 174 544 157 516 1.73e-65 222
MS.gene38196.t1 AT5G59450 31.383 376 254 3 173 544 223 598 1.20e-64 222
MS.gene38196.t1 AT2G29060 32.267 375 250 2 173 543 280 654 1.21e-64 223
MS.gene38196.t1 AT2G29060 32.095 377 252 2 171 543 315 691 2.06e-64 223
MS.gene38196.t1 AT2G37650 31.903 373 251 2 173 544 331 701 2.79e-61 214
MS.gene38196.t1 AT2G37650 31.903 373 251 2 173 544 343 713 3.72e-61 214
MS.gene38196.t1 AT3G46600 31.452 372 253 2 174 544 1 371 4.46e-61 206
MS.gene38196.t1 AT1G50420 29.792 433 235 7 174 544 54 479 8.63e-61 208
MS.gene38196.t1 AT3G46600 31.367 373 254 2 173 544 208 579 4.82e-60 209
MS.gene38196.t1 AT3G46600 31.367 373 254 2 173 544 176 547 6.81e-60 207
MS.gene38196.t1 AT2G29065 32.461 382 245 7 173 543 248 627 3.40e-56 199
MS.gene38196.t1 AT3G54220 34.605 367 222 7 181 543 297 649 3.77e-56 199
MS.gene38196.t1 AT1G07520 29.371 429 288 6 125 543 105 528 2.36e-55 195
MS.gene38196.t1 AT1G07520 29.371 429 288 6 125 543 105 528 2.36e-55 195
MS.gene38196.t1 AT1G07520 29.371 429 288 6 125 543 269 692 9.27e-55 196
MS.gene38196.t1 AT1G07520 29.371 429 288 6 125 543 270 693 9.58e-55 196
MS.gene38196.t1 AT5G66770 34.595 370 227 8 181 544 224 584 1.16e-51 186
MS.gene38196.t1 AT5G41920 32.718 379 231 9 173 544 39 400 3.13e-48 172
MS.gene38196.t1 AT3G50650 35.294 357 207 9 200 544 198 542 1.02e-47 174
MS.gene38196.t1 AT4G37650 29.104 402 247 12 170 544 139 529 1.29e-47 174
MS.gene38196.t1 AT1G55580 30.994 342 204 8 226 544 113 445 1.19e-44 164
MS.gene38196.t1 AT1G63100 32.665 349 217 6 174 515 249 586 5.40e-44 165
MS.gene38196.t1 AT1G63100 32.665 349 217 6 174 515 274 611 8.33e-44 165
MS.gene38196.t1 AT4G08250 27.816 435 280 10 130 543 55 476 8.99e-40 151
MS.gene38196.t1 AT3G49950 25.389 386 267 9 174 543 27 407 3.06e-31 125
MS.gene38196.t1 AT2G45160 25.532 376 231 13 191 544 291 639 5.73e-27 115
MS.gene38196.t1 AT3G60630 28.571 301 190 8 256 544 335 622 9.99e-25 108
MS.gene38196.t1 AT3G13840 27.749 382 247 12 175 544 146 510 3.74e-24 106
MS.gene38196.t1 AT4G36710 25.000 380 252 12 178 544 126 485 1.45e-20 95.1
MS.gene38196.t1 AT4G00150 23.098 368 253 11 182 544 212 554 3.40e-20 94.7
MS.gene38196.t1 AT5G67411 33.929 112 72 2 370 481 89 198 1.21e-11 64.7

Find 121 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCAGATGGTGTCGGTTTC+TGG 0.116927 5.3:+78806359 MS.gene38196:CDS
GTTTGCCCTTACTTCAAATT+TGG 0.195361 5.3:+78806527 MS.gene38196:CDS
ATTGGCTTCCTTGAGCAATT+TGG 0.205067 5.3:-78806619 None:intergenic
CAACAAGTGTCACGACTTTC+GGG 0.226734 5.3:-78806976 None:intergenic
CATTTCAAGCGAAGCGAAAT+TGG 0.230694 5.3:-78806213 None:intergenic
CCAATGGATTTCGTTGATTC+AGG 0.238320 5.3:+78806637 MS.gene38196:CDS
TCAACAAGTGTCACGACTTT+CGG 0.277772 5.3:-78806977 None:intergenic
TCCTTACCCTCTGAGTTCTT+TGG 0.281649 5.3:+78807246 MS.gene38196:CDS
CAATTTCGCTTCGCTTGAAA+TGG 0.314726 5.3:+78806214 MS.gene38196:CDS
CAAGCACAAGCTTAGAGAAT+TGG 0.336532 5.3:+78806124 MS.gene38196:CDS
CAATGTTGAGCAGCATTGTT+TGG 0.339609 5.3:+78807120 MS.gene38196:CDS
CCACCACACATCCGGATTAC+AGG 0.342966 5.3:+78806683 MS.gene38196:CDS
TAGCATGAATGCGAAATTTA+CGG 0.352242 5.3:-78806879 None:intergenic
GTGTCAGCACTCAGAATCAT+AGG 0.362236 5.3:+78806921 MS.gene38196:CDS
ATCATTGTCTGCAATTGCTT+TGG 0.368256 5.3:-78806303 None:intergenic
TCGATATTGCGGCAAGCAAT+AGG 0.368619 5.3:-78805823 None:intergenic
AATGGTTGATGGACGAATTA+CGG 0.368703 5.3:+78806339 MS.gene38196:CDS
TGGTGAACCGATTCAAAGAT+TGG 0.393469 5.3:+78806379 MS.gene38196:CDS
CATAACCTTGCGGTTCGGCC+AGG 0.394232 5.3:+78806845 MS.gene38196:CDS
CTTGAAGCTACTTAAATCTT+CGG 0.395656 5.3:-78806105 None:intergenic
CCGGATGTGTGGTGGTCCCC+CGG 0.395807 5.3:-78806675 None:intergenic
CCTCTGAGTTCTTTGGTGAA+TGG 0.405916 5.3:+78807253 MS.gene38196:CDS
GTTGCACGGTTGTCTGCATC+CGG 0.415709 5.3:+78806425 MS.gene38196:CDS
TGCAGACAATGATTTGCTAA+TGG 0.419025 5.3:+78806313 MS.gene38196:CDS
ATTTGGTTGCTTCTTGTGCT+TGG 0.429491 5.3:+78807365 MS.gene38196:CDS
ATGTAACCAAATTTGAAGTA+AGG 0.431720 5.3:-78806533 None:intergenic
TTTGGTTACATGTCTGCAAA+TGG 0.433675 5.3:+78806545 MS.gene38196:CDS
GTTATGCACGATCCACTCAT+TGG 0.435852 5.3:-78806083 None:intergenic
GCTAATGGCGCAATGGTTGA+TGG 0.437770 5.3:+78806328 MS.gene38196:CDS
TGAGATTCTTGCTGCGACAT+TGG 0.437774 5.3:-78806011 None:intergenic
TTTCACGCTGCAGCTATCTC+TGG 0.438144 5.3:+78806806 MS.gene38196:CDS
AAAGCTTGCTCGGCATTTCA+AGG 0.448893 5.3:+78806772 MS.gene38196:CDS
AGATTTGTAGATTGAACTCC+CGG 0.451937 5.3:-78806444 None:intergenic
GGCGAGCCATAGGTATTGTC+GGG 0.452043 5.3:-78806062 None:intergenic
ACTCCAACGTGCAGTAATAA+AGG 0.452114 5.3:-78805917 None:intergenic
TTGGAGTCGTCCTTGGCAGC+TGG 0.456230 5.3:+78805933 MS.gene38196:CDS
TGGTTGGATGAATAGAGATT+TGG 0.456807 5.3:+78807348 MS.gene38196:CDS
AACTGCATAACCTTGCGGTT+CGG 0.459814 5.3:+78806840 MS.gene38196:CDS
TCAGCTTATGCCCGTGGAGG+TGG 0.460311 5.3:+78806722 MS.gene38196:CDS
ATCCTCTCTTTATGCTCTCT+CGG 0.463692 5.3:-78807097 None:intergenic
AGGTCAAGATTTGCAATGGC+TGG 0.465717 5.3:+78807217 MS.gene38196:CDS
TGGCGAGCCATAGGTATTGT+CGG 0.470434 5.3:-78806063 None:intergenic
GAGAGAAGCTCGGCACTCTC+TGG 0.471288 5.3:-78806482 None:intergenic
TATGCTCCCAATCTTTGAAT+CGG 0.477205 5.3:-78806386 None:intergenic
AGAGATGGAGCTCTATATCT+TGG 0.480538 5.3:+78807328 MS.gene38196:CDS
TAGGCTTCAATTTGCTGCAA+TGG 0.480682 5.3:-78805804 None:intergenic
TGAGTGCTGACACTCTCATC+TGG 0.480842 5.3:-78806911 None:intergenic
AGGCCAAGCAACTCAATTCT+CGG 0.481276 5.3:+78805878 MS.gene38196:CDS
GGTTCGGCCAGGGGAAGCTC+TGG 0.484150 5.3:+78806856 MS.gene38196:CDS
GGTGAACCGATTCAAAGATT+GGG 0.489961 5.3:+78806380 MS.gene38196:CDS
CTGCACGTTGGAGTCGTCCT+TGG 0.491134 5.3:+78805926 MS.gene38196:CDS
ATAACCTTGCGGTTCGGCCA+GGG 0.491957 5.3:+78806846 MS.gene38196:CDS
GAAATCGATTATATGAACTC+TGG 0.496688 5.3:-78806597 None:intergenic
GAGCCGAGAATTGAGTTGCT+TGG 0.498447 5.3:-78805881 None:intergenic
TAATAAAGGTCAGAAGAAGT+TGG 0.499526 5.3:-78805903 None:intergenic
CATCAGCTGTACAACAATGA+TGG 0.501721 5.3:+78805855 MS.gene38196:CDS
AACAAAATTTCCAGCTGCCA+AGG 0.505944 5.3:-78805943 None:intergenic
CTTCGCTTGAAATGGACAGT+TGG 0.507924 5.3:+78806222 MS.gene38196:CDS
ACCAAAGAACTCAGAGGGTA+AGG 0.509605 5.3:-78807247 None:intergenic
GATCATCAATACCTGTAATC+CGG 0.510867 5.3:-78806694 None:intergenic
GACACGAGCTGCTAGGGAAG+TGG 0.514693 5.3:+78807194 MS.gene38196:CDS
GTGTCACGACTTTCGGGGAT+AGG 0.518113 5.3:-78806970 None:intergenic
TCCACTCATTGGCGAGCCAT+AGG 0.519637 5.3:-78806072 None:intergenic
TGGAACGACACGAGCTGCTA+GGG 0.521228 5.3:+78807188 MS.gene38196:CDS
GTGGAACGACACGAGCTGCT+AGG 0.524981 5.3:+78807187 MS.gene38196:CDS
ATGGAGCTCTATATCTTGGT+TGG 0.526117 5.3:+78807332 MS.gene38196:CDS
TTGCTTTGGCACATGCAGTA+AGG 0.529396 5.3:-78806289 None:intergenic
GTTAACATCATAGCTTGTGA+AGG 0.534917 5.3:+78807154 MS.gene38196:CDS
CGAATTACGGCAGATGGTGT+CGG 0.542149 5.3:+78806352 MS.gene38196:CDS
TGATTTAGATTTCGATATTG+CGG 0.543411 5.3:-78805834 None:intergenic
GCTGCAGCGTGAAACTCAAA+TGG 0.545283 5.3:-78806797 None:intergenic
TGTAACCAAATTTGAAGTAA+GGG 0.545514 5.3:-78806532 None:intergenic
TAACCTTGCGGTTCGGCCAG+GGG 0.545938 5.3:+78806847 MS.gene38196:CDS
ACCTATGGCTCGCCAATGAG+TGG 0.546193 5.3:+78806071 MS.gene38196:CDS
ATCGATTTCCAAATTGCTCA+AGG 0.546444 5.3:+78806611 MS.gene38196:CDS
GCACTCAGAATCATAGGGAT+AGG 0.551891 5.3:+78806927 MS.gene38196:CDS
TTAACATCATAGCTTGTGAA+GGG 0.552742 5.3:+78807155 MS.gene38196:CDS
CCTGAATCAACGAAATCCAT+TGG 0.554910 5.3:-78806637 None:intergenic
GGGGAATGATACTTATCAGA+TGG 0.562828 5.3:-78806041 None:intergenic
TGCGACATTGGACTATCGTT+AGG 0.563587 5.3:-78805999 None:intergenic
TTTACGGCCAGAGCTTCCCC+TGG 0.565827 5.3:-78806863 None:intergenic
GAATTGGAAAATGTAATGCT+TGG 0.570289 5.3:+78806140 MS.gene38196:CDS
CTTCCCCTGGCCGAACCGCA+AGG 0.580073 5.3:-78806850 None:intergenic
TTGCACGGTTGTCTGCATCC+GGG 0.585418 5.3:+78806426 MS.gene38196:CDS
TGTTGGAAGGACTCGTTGCA+CGG 0.585796 5.3:+78806411 MS.gene38196:CDS
TATATGTAGTCCACCTCCAC+GGG 0.589655 5.3:-78806732 None:intergenic
TATAGACTTGAAGAGAGAGA+TGG 0.590094 5.3:+78807313 MS.gene38196:CDS
TTGTGAAGGGGTCGAGAGAG+TGG 0.594827 5.3:+78807168 MS.gene38196:CDS
GACTACATATAGTTGAAAAG+AGG 0.595404 5.3:+78806744 MS.gene38196:CDS
TGTCAGCACTCAGAATCATA+GGG 0.595636 5.3:+78806922 MS.gene38196:CDS
AGAGGTTATCAAAGCTTGCT+CGG 0.596778 5.3:+78806762 MS.gene38196:CDS
AAGATTGGGAGCATACATGT+TGG 0.605912 5.3:+78806394 MS.gene38196:CDS
CTATATGTAGTCCACCTCCA+CGG 0.608482 5.3:-78806733 None:intergenic
TCCATAGTTTCGAGAAAACG+TGG 0.611195 5.3:-78807034 None:intergenic
GTGGAGGTCAAGATTTGCAA+TGG 0.615832 5.3:+78807213 MS.gene38196:CDS
TTGGGAGCATACATGTTGGA+AGG 0.617711 5.3:+78806398 MS.gene38196:CDS
TACGACAGTGCTATCAGCAA+CGG 0.617830 5.3:+78806188 MS.gene38196:CDS
TGACCTTTATTACTGCACGT+TGG 0.620270 5.3:+78805914 MS.gene38196:CDS
GGCGAGAAACTAACAGTCGA+AGG 0.623572 5.3:-78805978 None:intergenic
AAATTGCTCAAGGAAGCCAA+TGG 0.626215 5.3:+78806621 MS.gene38196:CDS
GATGGACGAATTACGGCAGA+TGG 0.627072 5.3:+78806346 MS.gene38196:CDS
TTACATGAACATACTTTACG+AGG 0.627108 5.3:+78806505 MS.gene38196:CDS
ACGAGCTGCTAGGGAAGTGG+AGG 0.634312 5.3:+78807197 MS.gene38196:CDS
CCACGGGCATAAGCTGATGT+CGG 0.634703 5.3:-78806716 None:intergenic
CATTCACCAAAGAACTCAGA+GGG 0.637813 5.3:-78807252 None:intergenic
CCGACATCAGCTTATGCCCG+TGG 0.638695 5.3:+78806716 MS.gene38196:CDS
ATGATTTGCTAATGGCGCAA+TGG 0.646542 5.3:+78806321 MS.gene38196:CDS
TTCCGAGAGAGCATAAAGAG+AGG 0.651166 5.3:+78807095 MS.gene38196:CDS
GCGAGCCATAGGTATTGTCG+GGG 0.657718 5.3:-78806061 None:intergenic
CAGCTGTACAACAATGATGG+AGG 0.659045 5.3:+78805858 MS.gene38196:CDS
TGTTCAACTGCATAACCTTG+CGG 0.660074 5.3:+78806835 MS.gene38196:CDS
ACATCAGCTTATGCCCGTGG+AGG 0.663564 5.3:+78806719 MS.gene38196:CDS
TAACATCATAGCTTGTGAAG+GGG 0.668817 5.3:+78807156 MS.gene38196:CDS
CCTGTAATCCGGATGTGTGG+TGG 0.675147 5.3:-78806683 None:intergenic
GGACAGTTGGAGACAAACAA+TGG 0.681512 5.3:+78806235 MS.gene38196:CDS
AACAAGTGTCACGACTTTCG+GGG 0.682943 5.3:-78806975 None:intergenic
GTTCATGTAAGAGAGAAGCT+CGG 0.686963 5.3:-78806492 None:intergenic
CCATTCACCAAAGAACTCAG+AGG 0.710837 5.3:-78807253 None:intergenic
ATACCTGTAATCCGGATGTG+TGG 0.719840 5.3:-78806686 None:intergenic
CGAGCCATAGGTATTGTCGG+GGG 0.727707 5.3:-78806060 None:intergenic
AGAATCTAACACAAACACTG+CGG 0.784454 5.3:+78807003 MS.gene38196:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTTAGATTTCGATATTG+CGG - chr5.3:78805837-78805856 None:intergenic 25.0%
!! ATGTAACCAAATTTGAAGTA+AGG - chr5.3:78806536-78806555 None:intergenic 25.0%
!! TGTAACCAAATTTGAAGTAA+GGG - chr5.3:78806535-78806554 None:intergenic 25.0%
!!! GTTTTCAAGCAATTTCTTTA+TGG - chr5.3:78807281-78807300 None:intergenic 25.0%
CTTGAAGCTACTTAAATCTT+CGG - chr5.3:78806108-78806127 None:intergenic 30.0%
GAAATCGATTATATGAACTC+TGG - chr5.3:78806600-78806619 None:intergenic 30.0%
GAATTGGAAAATGTAATGCT+TGG + chr5.3:78806140-78806159 MS.gene38196:CDS 30.0%
GACTACATATAGTTGAAAAG+AGG + chr5.3:78806744-78806763 MS.gene38196:CDS 30.0%
TAATAAAGGTCAGAAGAAGT+TGG - chr5.3:78805906-78805925 None:intergenic 30.0%
TAGCATGAATGCGAAATTTA+CGG - chr5.3:78806882-78806901 None:intergenic 30.0%
TTACATGAACATACTTTACG+AGG + chr5.3:78806505-78806524 MS.gene38196:CDS 30.0%
! TTAACATCATAGCTTGTGAA+GGG + chr5.3:78807155-78807174 MS.gene38196:CDS 30.0%
AGAATCTAACACAAACACTG+CGG + chr5.3:78807003-78807022 MS.gene38196:CDS 35.0%
AGATTTGTAGATTGAACTCC+CGG - chr5.3:78806447-78806466 None:intergenic 35.0%
ATCATTGTCTGCAATTGCTT+TGG - chr5.3:78806306-78806325 None:intergenic 35.0%
GATCATCAATACCTGTAATC+CGG - chr5.3:78806697-78806716 None:intergenic 35.0%
GTTTGCCCTTACTTCAAATT+TGG + chr5.3:78806527-78806546 MS.gene38196:CDS 35.0%
TATAGACTTGAAGAGAGAGA+TGG + chr5.3:78807313-78807332 MS.gene38196:CDS 35.0%
TATGCTCCCAATCTTTGAAT+CGG - chr5.3:78806389-78806408 None:intergenic 35.0%
TGGTTGGATGAATAGAGATT+TGG + chr5.3:78807348-78807367 MS.gene38196:CDS 35.0%
TTTGGTTACATGTCTGCAAA+TGG + chr5.3:78806545-78806564 MS.gene38196:CDS 35.0%
! ATCATAGGGATAGGCTTTTA+AGG + chr5.3:78806936-78806955 MS.gene38196:CDS 35.0%
! ATCGATTTCCAAATTGCTCA+AGG + chr5.3:78806611-78806630 MS.gene38196:CDS 35.0%
! ATTTTTCATGGCTTCTGCAA+TGG - chr5.3:78806573-78806592 None:intergenic 35.0%
! CTATCAAGATTTTCAGAGTC+AGG - chr5.3:78806167-78806186 None:intergenic 35.0%
! GTTAACATCATAGCTTGTGA+AGG + chr5.3:78807154-78807173 MS.gene38196:CDS 35.0%
! TAACATCATAGCTTGTGAAG+GGG + chr5.3:78807156-78807175 MS.gene38196:CDS 35.0%
!! AACTCTGGCTTCATTTTTCA+TGG - chr5.3:78806585-78806604 None:intergenic 35.0%
!! AATGGTTGATGGACGAATTA+CGG + chr5.3:78806339-78806358 MS.gene38196:CDS 35.0%
!! TGCAGACAATGATTTGCTAA+TGG + chr5.3:78806313-78806332 MS.gene38196:CDS 35.0%
AAATTGCTCAAGGAAGCCAA+TGG + chr5.3:78806621-78806640 MS.gene38196:CDS 40.0%
AACAAAATTTCCAGCTGCCA+AGG - chr5.3:78805946-78805965 None:intergenic 40.0%
ACTCCAACGTGCAGTAATAA+AGG - chr5.3:78805920-78805939 None:intergenic 40.0%
AGAGATGGAGCTCTATATCT+TGG + chr5.3:78807328-78807347 MS.gene38196:CDS 40.0%
ATCCTCTCTTTATGCTCTCT+CGG - chr5.3:78807100-78807119 None:intergenic 40.0%
ATGGAGCTCTATATCTTGGT+TGG + chr5.3:78807332-78807351 MS.gene38196:CDS 40.0%
ATTTGGTTGCTTCTTGTGCT+TGG + chr5.3:78807365-78807384 MS.gene38196:CDS 40.0%
CAAGCACAAGCTTAGAGAAT+TGG + chr5.3:78806124-78806143 MS.gene38196:CDS 40.0%
CAATGTTGAGCAGCATTGTT+TGG + chr5.3:78807120-78807139 MS.gene38196:CDS 40.0%
CAATTTCGCTTCGCTTGAAA+TGG + chr5.3:78806214-78806233 MS.gene38196:CDS 40.0%
CATCAGCTGTACAACAATGA+TGG + chr5.3:78805855-78805874 MS.gene38196:CDS 40.0%
CATTCACCAAAGAACTCAGA+GGG - chr5.3:78807255-78807274 None:intergenic 40.0%
CATTTCAAGCGAAGCGAAAT+TGG - chr5.3:78806216-78806235 None:intergenic 40.0%
CCTGAATCAACGAAATCCAT+TGG - chr5.3:78806640-78806659 None:intergenic 40.0%
GGGGAATGATACTTATCAGA+TGG - chr5.3:78806044-78806063 None:intergenic 40.0%
GTTCATGTAAGAGAGAAGCT+CGG - chr5.3:78806495-78806514 None:intergenic 40.0%
TCAACAAGTGTCACGACTTT+CGG - chr5.3:78806980-78806999 None:intergenic 40.0%
TCCATAGTTTCGAGAAAACG+TGG - chr5.3:78807037-78807056 None:intergenic 40.0%
TGACCTTTATTACTGCACGT+TGG + chr5.3:78805914-78805933 MS.gene38196:CDS 40.0%
TGTCAGCACTCAGAATCATA+GGG + chr5.3:78806922-78806941 MS.gene38196:CDS 40.0%
TGTTCAACTGCATAACCTTG+CGG + chr5.3:78806835-78806854 MS.gene38196:CDS 40.0%
! AAGATTGGGAGCATACATGT+TGG + chr5.3:78806394-78806413 MS.gene38196:CDS 40.0%
! AGAGGTTATCAAAGCTTGCT+CGG + chr5.3:78806762-78806781 MS.gene38196:CDS 40.0%
! ATTGGCTTCCTTGAGCAATT+TGG - chr5.3:78806622-78806641 None:intergenic 40.0%
! GGTGAACCGATTCAAAGATT+GGG + chr5.3:78806380-78806399 MS.gene38196:CDS 40.0%
! TCCACGTTTTCTCGAAACTA+TGG + chr5.3:78807033-78807052 MS.gene38196:CDS 40.0%
! TGGTGAACCGATTCAAAGAT+TGG + chr5.3:78806379-78806398 MS.gene38196:CDS 40.0%
!! ATGATTTGCTAATGGCGCAA+TGG + chr5.3:78806321-78806340 MS.gene38196:CDS 40.0%
!! CCAATGGATTTCGTTGATTC+AGG + chr5.3:78806637-78806656 MS.gene38196:CDS 40.0%
!! TAGGCTTCAATTTGCTGCAA+TGG - chr5.3:78805807-78805826 None:intergenic 40.0%
!!! TAGGGATAGGCTTTTAAGGT+TGG + chr5.3:78806940-78806959 MS.gene38196:CDS 40.0%
AACAAGTGTCACGACTTTCG+GGG - chr5.3:78806978-78806997 None:intergenic 45.0%
AACTGCATAACCTTGCGGTT+CGG + chr5.3:78806840-78806859 MS.gene38196:CDS 45.0%
ACCAAAGAACTCAGAGGGTA+AGG - chr5.3:78807250-78807269 None:intergenic 45.0%
AGGCCAAGCAACTCAATTCT+CGG + chr5.3:78805878-78805897 MS.gene38196:CDS 45.0%
AGGTCAAGATTTGCAATGGC+TGG + chr5.3:78807217-78807236 MS.gene38196:CDS 45.0%
ATACCTGTAATCCGGATGTG+TGG - chr5.3:78806689-78806708 None:intergenic 45.0%
CAACAAGTGTCACGACTTTC+GGG - chr5.3:78806979-78806998 None:intergenic 45.0%
CAGCTGTACAACAATGATGG+AGG + chr5.3:78805858-78805877 MS.gene38196:CDS 45.0%
CCATTCACCAAAGAACTCAG+AGG - chr5.3:78807256-78807275 None:intergenic 45.0%
CCTCTGAGTTCTTTGGTGAA+TGG + chr5.3:78807253-78807272 MS.gene38196:CDS 45.0%
CTATATGTAGTCCACCTCCA+CGG - chr5.3:78806736-78806755 None:intergenic 45.0%
CTTCGCTTGAAATGGACAGT+TGG + chr5.3:78806222-78806241 MS.gene38196:CDS 45.0%
GCACTCAGAATCATAGGGAT+AGG + chr5.3:78806927-78806946 MS.gene38196:CDS 45.0%
GGACAGTTGGAGACAAACAA+TGG + chr5.3:78806235-78806254 MS.gene38196:CDS 45.0%
GTGGAGGTCAAGATTTGCAA+TGG + chr5.3:78807213-78807232 MS.gene38196:CDS 45.0%
GTGTCAGCACTCAGAATCAT+AGG + chr5.3:78806921-78806940 MS.gene38196:CDS 45.0%
GTTATGCACGATCCACTCAT+TGG - chr5.3:78806086-78806105 None:intergenic 45.0%
TACGACAGTGCTATCAGCAA+CGG + chr5.3:78806188-78806207 MS.gene38196:CDS 45.0%
TATATGTAGTCCACCTCCAC+GGG - chr5.3:78806735-78806754 None:intergenic 45.0%
TCCTTACCCTCTGAGTTCTT+TGG + chr5.3:78807246-78807265 MS.gene38196:CDS 45.0%
TCGATATTGCGGCAAGCAAT+AGG - chr5.3:78805826-78805845 None:intergenic 45.0%
TGAGATTCTTGCTGCGACAT+TGG - chr5.3:78806014-78806033 None:intergenic 45.0%
TGCGACATTGGACTATCGTT+AGG - chr5.3:78806002-78806021 None:intergenic 45.0%
TTGCTTTGGCACATGCAGTA+AGG - chr5.3:78806292-78806311 None:intergenic 45.0%
! AAAGCTTGCTCGGCATTTCA+AGG + chr5.3:78806772-78806791 MS.gene38196:CDS 45.0%
! TGATTCAGGCTTTTGCAGCT+AGG + chr5.3:78806651-78806670 MS.gene38196:CDS 45.0%
! TTCCGAGAGAGCATAAAGAG+AGG + chr5.3:78807095-78807114 MS.gene38196:CDS 45.0%
! TTGGGAGCATACATGTTGGA+AGG + chr5.3:78806398-78806417 MS.gene38196:CDS 45.0%
CATTCCCCCGACAATACCTA+TGG + chr5.3:78806056-78806075 MS.gene38196:CDS 50.0%
CGAATTACGGCAGATGGTGT+CGG + chr5.3:78806352-78806371 MS.gene38196:CDS 50.0%
GATGGACGAATTACGGCAGA+TGG + chr5.3:78806346-78806365 MS.gene38196:CDS 50.0%
GCTAATGGCGCAATGGTTGA+TGG + chr5.3:78806328-78806347 MS.gene38196:CDS 50.0%
GCTGCAGCGTGAAACTCAAA+TGG - chr5.3:78806800-78806819 None:intergenic 50.0%
GGCGAGAAACTAACAGTCGA+AGG - chr5.3:78805981-78806000 None:intergenic 50.0%
TGTTGGAAGGACTCGTTGCA+CGG + chr5.3:78806411-78806430 MS.gene38196:CDS 50.0%
TTTCACGCTGCAGCTATCTC+TGG + chr5.3:78806806-78806825 MS.gene38196:CDS 50.0%
! GAGCCGAGAATTGAGTTGCT+TGG - chr5.3:78805884-78805903 None:intergenic 50.0%
! TGGCGAGCCATAGGTATTGT+CGG - chr5.3:78806066-78806085 None:intergenic 50.0%
!! TGAGTGCTGACACTCTCATC+TGG - chr5.3:78806914-78806933 None:intergenic 50.0%
ACATCAGCTTATGCCCGTGG+AGG + chr5.3:78806719-78806738 MS.gene38196:CDS 55.0%
ACCTATGGCTCGCCAATGAG+TGG + chr5.3:78806071-78806090 MS.gene38196:CDS 55.0%
CCACCACACATCCGGATTAC+AGG + chr5.3:78806683-78806702 MS.gene38196:CDS 55.0%
CCACGGGCATAAGCTGATGT+CGG - chr5.3:78806719-78806738 None:intergenic 55.0%
CCTGTAATCCGGATGTGTGG+TGG - chr5.3:78806686-78806705 None:intergenic 55.0%
GTGTCACGACTTTCGGGGAT+AGG - chr5.3:78806973-78806992 None:intergenic 55.0%
GTTGCACGGTTGTCTGCATC+CGG + chr5.3:78806425-78806444 MS.gene38196:CDS 55.0%
TCCACTCATTGGCGAGCCAT+AGG - chr5.3:78806075-78806094 None:intergenic 55.0%
TGGAACGACACGAGCTGCTA+GGG + chr5.3:78807188-78807207 MS.gene38196:CDS 55.0%
TTGCACGGTTGTCTGCATCC+GGG + chr5.3:78806426-78806445 MS.gene38196:CDS 55.0%
TTGTGAAGGGGTCGAGAGAG+TGG + chr5.3:78807168-78807187 MS.gene38196:CDS 55.0%
! ATAACCTTGCGGTTCGGCCA+GGG + chr5.3:78806846-78806865 MS.gene38196:CDS 55.0%
! CGAGCCATAGGTATTGTCGG+GGG - chr5.3:78806063-78806082 None:intergenic 55.0%
! GCGAGCCATAGGTATTGTCG+GGG - chr5.3:78806064-78806083 None:intergenic 55.0%
! GGCGAGCCATAGGTATTGTC+GGG - chr5.3:78806065-78806084 None:intergenic 55.0%
ACGAGCTGCTAGGGAAGTGG+AGG + chr5.3:78807197-78807216 MS.gene38196:CDS 60.0%
CCGACATCAGCTTATGCCCG+TGG + chr5.3:78806716-78806735 MS.gene38196:CDS 60.0%
CTGCACGTTGGAGTCGTCCT+TGG + chr5.3:78805926-78805945 MS.gene38196:CDS 60.0%
GACACGAGCTGCTAGGGAAG+TGG + chr5.3:78807194-78807213 MS.gene38196:CDS 60.0%
GAGAGAAGCTCGGCACTCTC+TGG - chr5.3:78806485-78806504 None:intergenic 60.0%
GTGGAACGACACGAGCTGCT+AGG + chr5.3:78807187-78807206 MS.gene38196:CDS 60.0%
TCAGCTTATGCCCGTGGAGG+TGG + chr5.3:78806722-78806741 MS.gene38196:CDS 60.0%
TTGGAGTCGTCCTTGGCAGC+TGG + chr5.3:78805933-78805952 MS.gene38196:CDS 60.0%
TTTACGGCCAGAGCTTCCCC+TGG - chr5.3:78806866-78806885 None:intergenic 60.0%
! AGGCTTTTGCAGCTAGGCCC+GGG + chr5.3:78806657-78806676 MS.gene38196:CDS 60.0%
! CAGGCTTTTGCAGCTAGGCC+CGG + chr5.3:78806656-78806675 MS.gene38196:CDS 60.0%
! CATAACCTTGCGGTTCGGCC+AGG + chr5.3:78806845-78806864 MS.gene38196:CDS 60.0%
! TAACCTTGCGGTTCGGCCAG+GGG + chr5.3:78806847-78806866 MS.gene38196:CDS 60.0%
!! CGGCAGATGGTGTCGGTTTC+TGG + chr5.3:78806359-78806378 MS.gene38196:CDS 60.0%
! GCTTTTGCAGCTAGGCCCGG+GGG + chr5.3:78806659-78806678 MS.gene38196:CDS 65.0%
! GGCTTTTGCAGCTAGGCCCG+GGG + chr5.3:78806658-78806677 MS.gene38196:CDS 65.0%
CCGGATGTGTGGTGGTCCCC+CGG - chr5.3:78806678-78806697 None:intergenic 70.0%
CCGGGGGACCACCACACATC+CGG + chr5.3:78806675-78806694 MS.gene38196:CDS 70.0%
CGGATGTGTGGTGGTCCCCC+GGG - chr5.3:78806677-78806696 None:intergenic 70.0%
CTTCCCCTGGCCGAACCGCA+AGG - chr5.3:78806853-78806872 None:intergenic 70.0%
GGTTCGGCCAGGGGAAGCTC+TGG + chr5.3:78806856-78806875 MS.gene38196:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 78805759 78807393 78805759 ID=MS.gene38196
chr5.3 mRNA 78805759 78807393 78805759 ID=MS.gene38196.t1;Parent=MS.gene38196
chr5.3 exon 78805759 78807393 78805759 ID=MS.gene38196.t1.exon1;Parent=MS.gene38196.t1
chr5.3 CDS 78805759 78807393 78805759 ID=cds.MS.gene38196.t1;Parent=MS.gene38196.t1
Gene Sequence

>MS.gene38196

ATGCAAGCATCAGAGCAGCATAGAAGTTCAAGTATGTACTATCAACCATTGCAGCAAATTGAAGCCTATTGCTTGCCGCAATATCGAAATCTAAATCATCAGCTGTACAACAATGATGGAGGCCAAGCAACTCAATTCTCGGCTCCAACTTCTTCTGACCTTTATTACTGCACGTTGGAGTCGTCCTTGGCAGCTGGAAATTTTGTTGTTTACAACTCTCCTTCGACTGTTAGTTTCTCGCCTAACGATAGTCCAATGTCGCAGCAAGAATCTCAGTCTTATCCATCTGATAAGTATCATTCCCCCGACAATACCTATGGCTCGCCAATGAGTGGATCGTGCATAACCGAAGATTTAAGTAGCTTCAAGCACAAGCTTAGAGAATTGGAAAATGTAATGCTTGGTCCTGACTCTGAAAATCTTGATAGTTACGACAGTGCTATCAGCAACGGAACCAATTTCGCTTCGCTTGAAATGGACAGTTGGAGACAAACAATGGTAGCAATTTCGAGCAAAAATCTAAAGCACATCCTTACTGCATGTGCCAAAGCAATTGCAGACAATGATTTGCTAATGGCGCAATGGTTGATGGACGAATTACGGCAGATGGTGTCGGTTTCTGGTGAACCGATTCAAAGATTGGGAGCATACATGTTGGAAGGACTCGTTGCACGGTTGTCTGCATCCGGGAGTTCAATCTACAAATCTTTAAGATGCAAAGAACCAGAGAGTGCCGAGCTTCTCTCTTACATGAACATACTTTACGAGGTTTGCCCTTACTTCAAATTTGGTTACATGTCTGCAAATGGAGCCATTGCAGAAGCCATGAAAAATGAAGCCAGAGTTCATATAATCGATTTCCAAATTGCTCAAGGAAGCCAATGGATTTCGTTGATTCAGGCTTTTGCAGCTAGGCCCGGGGGACCACCACACATCCGGATTACAGGTATTGATGATCCGACATCAGCTTATGCCCGTGGAGGTGGACTACATATAGTTGAAAAGAGGTTATCAAAGCTTGCTCGGCATTTCAAGGTGCCATTTGAGTTTCACGCTGCAGCTATCTCTGGTTGTGATGTTCAACTGCATAACCTTGCGGTTCGGCCAGGGGAAGCTCTGGCCGTAAATTTCGCATTCATGCTACATCACATGCCAGATGAGAGTGTCAGCACTCAGAATCATAGGGATAGGCTTTTAAGGTTGGTTAAGAGCCTATCCCCGAAAGTCGTGACACTTGTTGAGCAAGAATCTAACACAAACACTGCGGCATTCTTTCCACGTTTTCTCGAAACTATGGACTATTACACAGCAATGTTCGAGTCAATAGACGTGACTCTTCCGAGAGAGCATAAAGAGAGGATCAATGTTGAGCAGCATTGTTTGGCAAGAGATTTAGTTAACATCATAGCTTGTGAAGGGGTCGAGAGAGTGGAACGACACGAGCTGCTAGGGAAGTGGAGGTCAAGATTTGCAATGGCTGGTTTCACTCCTTACCCTCTGAGTTCTTTGGTGAATGGTACCATAAAGAAATTGCTTGAAAACTACAGTGATAGATATAGACTTGAAGAGAGAGATGGAGCTCTATATCTTGGTTGGATGAATAGAGATTTGGTTGCTTCTTGTGCTTGGAAATGA
Protein sequence

>MS.gene38196.t1

MQASEQHRSSSMYYQPLQQIEAYCLPQYRNLNHQLYNNDGGQATQFSAPTSSDLYYCTLESSLAAGNFVVYNSPSTVSFSPNDSPMSQQESQSYPSDKYHSPDNTYGSPMSGSCITEDLSSFKHKLRELENVMLGPDSENLDSYDSAISNGTNFASLEMDSWRQTMVAISSKNLKHILTACAKAIADNDLLMAQWLMDELRQMVSVSGEPIQRLGAYMLEGLVARLSASGSSIYKSLRCKEPESAELLSYMNILYEVCPYFKFGYMSANGAIAEAMKNEARVHIIDFQIAQGSQWISLIQAFAARPGGPPHIRITGIDDPTSAYARGGGLHIVEKRLSKLARHFKVPFEFHAAAISGCDVQLHNLAVRPGEALAVNFAFMLHHMPDESVSTQNHRDRLLRLVKSLSPKVVTLVEQESNTNTAAFFPRFLETMDYYTAMFESIDVTLPREHKERINVEQHCLARDLVNIIACEGVERVERHELLGKWRSRFAMAGFTPYPLSSLVNGTIKKLLENYSDRYRLEERDGALYLGWMNRDLVASCAWK