Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38243.t1 | AET00865.1 | 83.3 | 323 | 26 | 3 | 1 | 295 | 1 | 323 | 3.20E-144 | 521.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38243.t1 | Q680K8 | 39.2 | 329 | 153 | 7 | 4 | 294 | 4 | 323 | 2.7e-51 | 203.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38243.t1 | G7K4U5 | 83.3 | 323 | 26 | 3 | 1 | 295 | 1 | 323 | 2.3e-144 | 521.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene38243.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38243.t1 | MTR_5g096790 | 82.663 | 323 | 28 | 2 | 1 | 295 | 1 | 323 | 0.0 | 533 |
| MS.gene38243.t1 | MTR_5g096700 | 41.590 | 327 | 144 | 9 | 6 | 294 | 8 | 325 | 1.25e-71 | 224 |
| MS.gene38243.t1 | MTR_3g061090 | 42.169 | 332 | 146 | 8 | 1 | 294 | 1 | 324 | 1.44e-68 | 216 |
| MS.gene38243.t1 | MTR_5g096700 | 44.697 | 264 | 115 | 5 | 58 | 294 | 7 | 266 | 2.69e-65 | 206 |
| MS.gene38243.t1 | MTR_3g061090 | 44.314 | 255 | 112 | 4 | 67 | 294 | 1 | 252 | 3.81e-58 | 187 |
| MS.gene38243.t1 | MTR_3g088560 | 32.622 | 328 | 171 | 10 | 7 | 294 | 4 | 321 | 3.35e-44 | 153 |
| MS.gene38243.t1 | MTR_1g029850 | 31.402 | 328 | 177 | 10 | 7 | 294 | 5 | 324 | 2.17e-43 | 151 |
| MS.gene38243.t1 | MTR_3g088560 | 35.652 | 230 | 117 | 4 | 95 | 295 | 25 | 252 | 1.05e-36 | 132 |
| MS.gene38243.t1 | MTR_3g088560 | 35.808 | 229 | 116 | 4 | 95 | 294 | 40 | 266 | 2.58e-36 | 131 |
| MS.gene38243.t1 | MTR_3g088560 | 37.696 | 191 | 116 | 2 | 106 | 295 | 12 | 200 | 7.00e-36 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38243.t1 | AT1G55760 | 39.157 | 332 | 155 | 7 | 1 | 294 | 1 | 323 | 2.77e-65 | 207 |
| MS.gene38243.t1 | AT1G21780 | 35.276 | 326 | 163 | 10 | 7 | 294 | 5 | 320 | 5.05e-49 | 166 |
| MS.gene38243.t1 | AT1G21780 | 35.276 | 326 | 163 | 10 | 7 | 294 | 5 | 320 | 5.05e-49 | 166 |
Find 44 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCAATATCTAGTGGAATT+TGG | 0.233852 | 5.3:-78205709 | MS.gene38243:CDS |
| TTGGTAAAATACATGAAATC+TGG | 0.300649 | 5.3:-78205690 | MS.gene38243:CDS |
| GGTTTGCAGTCTTGAGATCT+AGG | 0.312817 | 5.3:+78206344 | None:intergenic |
| TCAAGATATCTAAATGGAAT+TGG | 0.352470 | 5.3:-78207284 | MS.gene38243:intron |
| GCGAAACTGATGCATTTAAC+TGG | 0.358814 | 5.3:-78206420 | MS.gene38243:CDS |
| AAGCTTTCAAGATATCTAAA+TGG | 0.359355 | 5.3:-78207290 | MS.gene38243:CDS |
| TATGTTTGCACAAGATTCTA+AGG | 0.372257 | 5.3:-78205995 | MS.gene38243:CDS |
| CCCACGGATTCCACTTTGAA+TGG | 0.379615 | 5.3:+78207384 | None:intergenic |
| GCATATAATTTGATAGTAAC+CGG | 0.381945 | 5.3:+78207007 | None:intergenic |
| TTTAGATATCTTGAAAGCTT+CGG | 0.402228 | 5.3:+78207293 | None:intergenic |
| GAATCATAGTTTCGAGCTAT+TGG | 0.408610 | 5.3:-78205872 | MS.gene38243:CDS |
| ATGAATGCTTGACAAACTTC+AGG | 0.409567 | 5.3:+78205934 | None:intergenic |
| ATCTAGCTGTTCTTGCTGCC+CGG | 0.416939 | 5.3:-78206035 | MS.gene38243:CDS |
| CGGAATTTGAAAGATGCAAT+TGG | 0.422404 | 5.3:+78206962 | None:intergenic |
| CCGTGGGTTACGTCGCTCAA+TGG | 0.441645 | 5.3:-78207368 | MS.gene38243:CDS |
| TGCAATTGGTGGATTATTTG+TGG | 0.455591 | 5.3:+78206976 | None:intergenic |
| GGAATAACCTTCGCAGAGAC+TGG | 0.460912 | 5.3:-78206914 | MS.gene38243:intron |
| TTCCGATAGAGTCAAGAATA+CGG | 0.466943 | 5.3:+78206942 | None:intergenic |
| TTCCGTATTCTTGACTCTAT+CGG | 0.469882 | 5.3:-78206944 | MS.gene38243:CDS |
| TGGTAAAATACATGAAATCT+GGG | 0.484453 | 5.3:-78205689 | MS.gene38243:CDS |
| TTGGTTGCTGCTGAGAAATA+CGG | 0.484832 | 5.3:-78205853 | MS.gene38243:CDS |
| AATTTGAAAGATGCAATTGG+TGG | 0.524532 | 5.3:+78206965 | None:intergenic |
| ACACAGGATGTTGTAACATC+AGG | 0.530431 | 5.3:+78206379 | None:intergenic |
| TTAACTAAGGAAGAAATTCA+CGG | 0.535488 | 5.3:+78205611 | None:intergenic |
| GTTAAATGCATCAGTTTCGC+TGG | 0.535589 | 5.3:+78206423 | None:intergenic |
| TATATTCAAACATAGGTGAC+CGG | 0.536701 | 5.3:+78206016 | None:intergenic |
| AGCGCAACTAATCTTCAAGT+AGG | 0.555426 | 5.3:+78205729 | None:intergenic |
| TCTTGAAAGCTTCGGATGTG+TGG | 0.556978 | 5.3:+78207301 | None:intergenic |
| TCCATTCAAAGTGGAATCCG+TGG | 0.560598 | 5.3:-78207385 | MS.gene38243:CDS |
| GCAAACATATATTCAAACAT+AGG | 0.563128 | 5.3:+78206009 | None:intergenic |
| CGAAACTGATGCATTTAACT+GGG | 0.567854 | 5.3:-78206419 | MS.gene38243:CDS |
| TCAAGCTCGTATCGTATCAT+CGG | 0.569700 | 5.3:-78206076 | MS.gene38243:CDS |
| CGGATGTGTGGATAAAGCTG+TGG | 0.593373 | 5.3:+78207313 | None:intergenic |
| GCGCAACTAATCTTCAAGTA+GGG | 0.602481 | 5.3:+78205730 | None:intergenic |
| TAGGAATTCAACTTCAACAC+AGG | 0.603549 | 5.3:+78206363 | None:intergenic |
| CCATTGAGCGACGTAACCCA+CGG | 0.604930 | 5.3:+78207368 | None:intergenic |
| CGGCGTTAATGATTTGAGAG+AGG | 0.605267 | 5.3:-78205833 | MS.gene38243:CDS |
| TAGTTGCGCTCAATATCTAG+TGG | 0.608180 | 5.3:-78205716 | MS.gene38243:CDS |
| GGATGTGTGGATAAAGCTGT+GGG | 0.609946 | 5.3:+78207314 | None:intergenic |
| CCATTCAAAGTGGAATCCGT+GGG | 0.610952 | 5.3:-78207384 | MS.gene38243:CDS |
| ATTGATAGAAATATGCAGCA+TGG | 0.618155 | 5.3:+78205755 | None:intergenic |
| ATATTCAAACATAGGTGACC+GGG | 0.654376 | 5.3:+78206017 | None:intergenic |
| GCTTGATTGTAGCGTCTGTG+TGG | 0.704769 | 5.3:+78206093 | None:intergenic |
| GATGTGTGGATAAAGCTGTG+GGG | 0.847745 | 5.3:+78207315 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTCAATTATATCATTA+AGG | - | chr5.3:78206184-78206203 | MS.gene38243:intron | 10.0% |
| !!! | ATAATTTTAGTATTGATATT+AGG | - | chr5.3:78206775-78206794 | MS.gene38243:intron | 10.0% |
| !! | ATTATAAGTATATATGTTTG+TGG | + | chr5.3:78206328-78206347 | None:intergenic | 15.0% |
| !!! | TGTTTAAATTTTTTGAATGA+AGG | - | chr5.3:78206831-78206850 | MS.gene38243:intron | 15.0% |
| !! | ATGCTTATATCTATATGAAT+AGG | - | chr5.3:78206388-78206407 | MS.gene38243:CDS | 20.0% |
| !! | ATTGAATAAAACAAGACATA+TGG | - | chr5.3:78206488-78206507 | MS.gene38243:intron | 20.0% |
| !! | CTCTAATCATAAAAATCATA+TGG | - | chr5.3:78205826-78205845 | MS.gene38243:CDS | 20.0% |
| !! | TGCTTATATCTATATGAATA+GGG | - | chr5.3:78206389-78206408 | MS.gene38243:CDS | 20.0% |
| !!! | TTTCATAGACAAAGATTTTT+AGG | + | chr5.3:78206451-78206470 | None:intergenic | 20.0% |
| ! | AACAAGACATATGGTTAAAT+AGG | - | chr5.3:78206497-78206516 | MS.gene38243:intron | 25.0% |
| ! | AAGCTTTCAAGATATCTAAA+TGG | - | chr5.3:78205721-78205740 | MS.gene38243:CDS | 25.0% |
| ! | ACAGAAAAAATATACTTGAG+AGG | - | chr5.3:78207231-78207250 | MS.gene38243:intron | 25.0% |
| ! | ATCATAAAAATCATATGGTC+TGG | - | chr5.3:78205831-78205850 | MS.gene38243:CDS | 25.0% |
| ! | CTTTCAGAATGTAACAAATT+AGG | + | chr5.3:78206305-78206324 | None:intergenic | 25.0% |
| ! | GCAAACATATATTCAAACAT+AGG | + | chr5.3:78207005-78207024 | None:intergenic | 25.0% |
| ! | TCAAGATATCTAAATGGAAT+TGG | - | chr5.3:78205727-78205746 | MS.gene38243:CDS | 25.0% |
| ! | TGGTAAAATACATGAAATCT+GGG | - | chr5.3:78207322-78207341 | MS.gene38243:CDS | 25.0% |
| ! | TTACTTATAGAACTATGAGT+TGG | - | chr5.3:78206152-78206171 | MS.gene38243:intron | 25.0% |
| ! | TTGGTAAAATACATGAAATC+TGG | - | chr5.3:78207321-78207340 | MS.gene38243:CDS | 25.0% |
| ! | TTTAGATATCTTGAAAGCTT+CGG | + | chr5.3:78205721-78205740 | None:intergenic | 25.0% |
| !! | GCATATAATTTGATAGTAAC+CGG | + | chr5.3:78206007-78206026 | None:intergenic | 25.0% |
| !!! | GTCTATGAAAATGTTGTTTT+TGG | - | chr5.3:78206461-78206480 | MS.gene38243:intron | 25.0% |
| AATTTGAAAGATGCAATTGG+TGG | + | chr5.3:78206049-78206068 | None:intergenic | 30.0% | |
| ATAGAGAAACTAACTCAACT+AGG | - | chr5.3:78206854-78206873 | MS.gene38243:intron | 30.0% | |
| ATTGATAGAAATATGCAGCA+TGG | + | chr5.3:78207259-78207278 | None:intergenic | 30.0% | |
| GATTAAATGCACGTACATTT+GGG | + | chr5.3:78206691-78206710 | None:intergenic | 30.0% | |
| TACCAACGTTATGATGAAAT+CGG | + | chr5.3:78205860-78205879 | None:intergenic | 30.0% | |
| TATATTCAAACATAGGTGAC+CGG | + | chr5.3:78206998-78207017 | None:intergenic | 30.0% | |
| TATGTTTGCACAAGATTCTA+AGG | - | chr5.3:78207016-78207035 | MS.gene38243:intron | 30.0% | |
| TGATTAAATGCACGTACATT+TGG | + | chr5.3:78206692-78206711 | None:intergenic | 30.0% | |
| TTCTCTTAACATTCGAACAA+GGG | + | chr5.3:78206893-78206912 | None:intergenic | 30.0% | |
| TTTCTCTTAACATTCGAACA+AGG | + | chr5.3:78206894-78206913 | None:intergenic | 30.0% | |
| ! | TTTTCTTGCCTTCCTAAAAT+TGG | - | chr5.3:78207349-78207368 | MS.gene38243:CDS | 30.0% |
| !!! | TTTTTTACTTCCCAACACAA+AGG | - | chr5.3:78205948-78205967 | MS.gene38243:CDS | 30.0% |
| AATGATATGTGCCTTTGTGT+TGG | + | chr5.3:78205962-78205981 | None:intergenic | 35.0% | |
| ATATTCAAACATAGGTGACC+GGG | + | chr5.3:78206997-78207016 | None:intergenic | 35.0% | |
| ATCCGATTTCATCATAACGT+TGG | - | chr5.3:78205855-78205874 | MS.gene38243:CDS | 35.0% | |
| ATGAATGCTTGACAAACTTC+AGG | + | chr5.3:78207080-78207099 | None:intergenic | 35.0% | |
| ATGATATGTGCCTTTGTGTT+GGG | + | chr5.3:78205961-78205980 | None:intergenic | 35.0% | |
| CGAAACTGATGCATTTAACT+GGG | - | chr5.3:78206592-78206611 | MS.gene38243:intron | 35.0% | |
| CGGAATTTGAAAGATGCAAT+TGG | + | chr5.3:78206052-78206071 | None:intergenic | 35.0% | |
| CTTGACTCTATCGGAAAAAA+TGG | - | chr5.3:78206076-78206095 | MS.gene38243:CDS | 35.0% | |
| GAATCATAGTTTCGAGCTAT+TGG | - | chr5.3:78207139-78207158 | MS.gene38243:intron | 35.0% | |
| GCTCAATATCTAGTGGAATT+TGG | - | chr5.3:78207302-78207321 | MS.gene38243:CDS | 35.0% | |
| TAGGAATTCAACTTCAACAC+AGG | + | chr5.3:78206651-78206670 | None:intergenic | 35.0% | |
| TATGAATAGGGACTATGAGT+TGG | - | chr5.3:78206401-78206420 | MS.gene38243:CDS | 35.0% | |
| TTCCGATAGAGTCAAGAATA+CGG | + | chr5.3:78206072-78206091 | None:intergenic | 35.0% | |
| TTCCGTATTCTTGACTCTAT+CGG | - | chr5.3:78206067-78206086 | MS.gene38243:CDS | 35.0% | |
| ! | CATCATCATCTGCCAATTTT+AGG | + | chr5.3:78207364-78207383 | None:intergenic | 35.0% |
| ! | CTTTTTCTTCTGCATCATTG+TGG | + | chr5.3:78206556-78206575 | None:intergenic | 35.0% |
| ! | GTTTTACTCTCCATTCAAAG+TGG | - | chr5.3:78205617-78205636 | MS.gene38243:CDS | 35.0% |
| ! | TAATTTGATAGTAACCGGAC+TGG | + | chr5.3:78206002-78206021 | None:intergenic | 35.0% |
| !! | ATCATCTGCCAATTTTAGGA+AGG | + | chr5.3:78207360-78207379 | None:intergenic | 35.0% |
| !! | TGCAATTGGTGGATTATTTG+TGG | + | chr5.3:78206038-78206057 | None:intergenic | 35.0% |
| AAGGCACATATCATTCTCGA+GGG | - | chr5.3:78205967-78205986 | MS.gene38243:CDS | 40.0% | |
| ACACAGGATGTTGTAACATC+AGG | + | chr5.3:78206635-78206654 | None:intergenic | 40.0% | |
| AGCGCAACTAATCTTCAAGT+AGG | + | chr5.3:78207285-78207304 | None:intergenic | 40.0% | |
| GCGAAACTGATGCATTTAAC+TGG | - | chr5.3:78206591-78206610 | MS.gene38243:intron | 40.0% | |
| GCGCAACTAATCTTCAAGTA+GGG | + | chr5.3:78207284-78207303 | None:intergenic | 40.0% | |
| GTTAAATGCATCAGTTTCGC+TGG | + | chr5.3:78206591-78206610 | None:intergenic | 40.0% | |
| TAGTTGCGCTCAATATCTAG+TGG | - | chr5.3:78207295-78207314 | MS.gene38243:CDS | 40.0% | |
| TCAAGCTCGTATCGTATCAT+CGG | - | chr5.3:78206935-78206954 | MS.gene38243:CDS | 40.0% | |
| TCTGCATCATTGTGGAAAAG+AGG | + | chr5.3:78206548-78206567 | None:intergenic | 40.0% | |
| TTGGTTGCTGCTGAGAAATA+CGG | - | chr5.3:78207158-78207177 | MS.gene38243:intron | 40.0% | |
| ! | AAAGGCACATATCATTCTCG+AGG | - | chr5.3:78205966-78205985 | MS.gene38243:CDS | 40.0% |
| !!! | TTTTTTACCAGTCTCTGCGA+AGG | + | chr5.3:78206107-78206126 | None:intergenic | 40.0% |
| CCATTCAAAGTGGAATCCGT+GGG | - | chr5.3:78205627-78205646 | MS.gene38243:CDS | 45.0% | |
| CGGCGTTAATGATTTGAGAG+AGG | - | chr5.3:78207178-78207197 | MS.gene38243:intron | 45.0% | |
| GATGTGTGGATAAAGCTGTG+GGG | + | chr5.3:78205699-78205718 | None:intergenic | 45.0% | |
| GGATGTGTGGATAAAGCTGT+GGG | + | chr5.3:78205700-78205719 | None:intergenic | 45.0% | |
| GGTTTGCAGTCTTGAGATCT+AGG | + | chr5.3:78206670-78206689 | None:intergenic | 45.0% | |
| TCCATTCAAAGTGGAATCCG+TGG | - | chr5.3:78205626-78205645 | MS.gene38243:CDS | 45.0% | |
| TCTTGAAAGCTTCGGATGTG+TGG | + | chr5.3:78205713-78205732 | None:intergenic | 45.0% | |
| ! | GATAGTAACCGGACTGGAAA+GGG | + | chr5.3:78205996-78206015 | None:intergenic | 45.0% |
| ! | TGATAGTAACCGGACTGGAA+AGG | + | chr5.3:78205997-78206016 | None:intergenic | 45.0% |
| ATCTAGCTGTTCTTGCTGCC+CGG | - | chr5.3:78206976-78206995 | MS.gene38243:CDS | 50.0% | |
| CCCACGGATTCCACTTTGAA+TGG | + | chr5.3:78205630-78205649 | None:intergenic | 50.0% | |
| CGGATGTGTGGATAAAGCTG+TGG | + | chr5.3:78205701-78205720 | None:intergenic | 50.0% | |
| GCTTGATTGTAGCGTCTGTG+TGG | + | chr5.3:78206921-78206940 | None:intergenic | 50.0% | |
| GGAATAACCTTCGCAGAGAC+TGG | - | chr5.3:78206097-78206116 | MS.gene38243:CDS | 50.0% | |
| CCATTGAGCGACGTAACCCA+CGG | + | chr5.3:78205646-78205665 | None:intergenic | 55.0% | |
| GAGGGATTCCCTTTCCAGTC+CGG | - | chr5.3:78205985-78206004 | MS.gene38243:CDS | 55.0% | |
| ! | CCGTGGGTTACGTCGCTCAA+TGG | - | chr5.3:78205643-78205662 | MS.gene38243:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 78205615 | 78207418 | 78205615 | ID=MS.gene38243 |
| chr5.3 | mRNA | 78205615 | 78207418 | 78205615 | ID=MS.gene38243.t1;Parent=MS.gene38243 |
| chr5.3 | exon | 78207285 | 78207418 | 78207285 | ID=MS.gene38243.t1.exon1;Parent=MS.gene38243.t1 |
| chr5.3 | CDS | 78207285 | 78207418 | 78207285 | ID=cds.MS.gene38243.t1;Parent=MS.gene38243.t1 |
| chr5.3 | exon | 78206915 | 78207030 | 78206915 | ID=MS.gene38243.t1.exon2;Parent=MS.gene38243.t1 |
| chr5.3 | CDS | 78206915 | 78207030 | 78206915 | ID=cds.MS.gene38243.t1;Parent=MS.gene38243.t1 |
| chr5.3 | exon | 78206339 | 78206466 | 78206339 | ID=MS.gene38243.t1.exon3;Parent=MS.gene38243.t1 |
| chr5.3 | CDS | 78206339 | 78206466 | 78206339 | ID=cds.MS.gene38243.t1;Parent=MS.gene38243.t1 |
| chr5.3 | exon | 78205615 | 78206124 | 78205615 | ID=MS.gene38243.t1.exon4;Parent=MS.gene38243.t1 |
| chr5.3 | CDS | 78205615 | 78206124 | 78205615 | ID=cds.MS.gene38243.t1;Parent=MS.gene38243.t1 |
| Gene Sequence |
| Protein sequence |
