Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38766.t1 | XP_003625012.1 | 100 | 153 | 0 | 0 | 1 | 153 | 1 | 153 | 3.10E-82 | 314.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38766.t1 | Q2HW56 | 100.0 | 153 | 0 | 0 | 1 | 153 | 1 | 153 | 7.5e-85 | 314.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38766.t1 | A0A2K3L0G6 | 98.0 | 152 | 2 | 1 | 1 | 152 | 1 | 151 | 1.5e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene38766.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38766.t1 | MTR_7g090020 | 100.000 | 153 | 0 | 0 | 1 | 153 | 1 | 153 | 1.42e-110 | 322 |
| MS.gene38766.t1 | MTR_1g051025 | 88.889 | 153 | 17 | 0 | 1 | 153 | 1 | 153 | 4.34e-96 | 286 |
| MS.gene38766.t1 | MTR_5g090770 | 33.548 | 155 | 80 | 5 | 2 | 153 | 54 | 188 | 1.90e-16 | 75.5 |
| MS.gene38766.t1 | MTR_5g090770 | 34.586 | 133 | 74 | 4 | 24 | 153 | 66 | 188 | 2.67e-16 | 75.1 |
| MS.gene38766.t1 | MTR_2g028950 | 33.557 | 149 | 91 | 4 | 6 | 153 | 44 | 185 | 4.18e-16 | 74.7 |
| MS.gene38766.t1 | MTR_2g028950 | 33.557 | 149 | 91 | 4 | 6 | 153 | 44 | 185 | 5.89e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38766.t1 | AT2G41370 | 79.375 | 160 | 25 | 2 | 2 | 153 | 3 | 162 | 2.71e-87 | 263 |
| MS.gene38766.t1 | AT3G57130 | 78.981 | 157 | 25 | 2 | 2 | 153 | 4 | 157 | 1.00e-85 | 249 |
| MS.gene38766.t1 | AT3G57130 | 78.981 | 157 | 25 | 2 | 2 | 153 | 4 | 157 | 8.61e-84 | 254 |
| MS.gene38766.t1 | AT5G45110 | 31.944 | 144 | 87 | 4 | 6 | 148 | 40 | 173 | 3.29e-12 | 63.5 |
| MS.gene38766.t1 | AT5G45110 | 31.944 | 144 | 87 | 4 | 6 | 148 | 40 | 173 | 5.30e-12 | 62.8 |
Find 39 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGGCCAGACCCACCTTC+TGG | 0.259914 | 7.4:-18813263 | MS.gene38766:CDS |
| CAACCACGGTCACCACAATT+AGG | 0.374903 | 7.4:+18813086 | None:intergenic |
| GAGATCTTAGTGAGTCTTCA+AGG | 0.426128 | 7.4:+18813420 | None:intergenic |
| GGGTTGACTCGGTTTCCTGA+TGG | 0.427587 | 7.4:+18813233 | None:intergenic |
| TCGTAACCAACTGAGTTAAC+TGG | 0.436058 | 7.4:+18813182 | None:intergenic |
| GGTTGACTCGGTTTCCTGAT+GGG | 0.440013 | 7.4:+18813234 | None:intergenic |
| AACACCAGACCTAGCTGAAC+CGG | 0.442219 | 7.4:+18813208 | None:intergenic |
| TTCTTCAGAAAATTCTTCTG+TGG | 0.443436 | 7.4:-18813281 | MS.gene38766:CDS |
| TCTAATCCAGAAGGTGGGTC+TGG | 0.456640 | 7.4:+18813257 | None:intergenic |
| GTTATACCAGTTAACTCAGT+TGG | 0.472537 | 7.4:-18813188 | MS.gene38766:CDS |
| ATTGTGGTGACCGTGGTTGT+TGG | 0.504368 | 7.4:-18813082 | MS.gene38766:CDS |
| TCTTCAGAAAATTCTTCTGT+GGG | 0.516918 | 7.4:-18813280 | MS.gene38766:CDS |
| ACGCTTGTCCGTTGATGAGA+AGG | 0.520055 | 7.4:+18813378 | None:intergenic |
| CCTTCTGGATTAGACCCATC+AGG | 0.521120 | 7.4:-18813248 | MS.gene38766:CDS |
| CCATCCGGTTCAGCTAGGTC+TGG | 0.539454 | 7.4:-18813212 | MS.gene38766:CDS |
| GGAAACCGAGTCAACCCATC+CGG | 0.539988 | 7.4:-18813227 | MS.gene38766:CDS |
| TACTTAAACCTTCTCATCAA+CGG | 0.546164 | 7.4:-18813386 | MS.gene38766:CDS |
| CTTGCAGCCGCTAGATACTT+CGG | 0.551437 | 7.4:-18813017 | MS.gene38766:CDS |
| CTGAACCGGATGGGTTGACT+CGG | 0.552813 | 7.4:+18813222 | None:intergenic |
| GGCCTAGGTTCATGTTTCTG+AGG | 0.555362 | 7.4:+18813107 | None:intergenic |
| ACAGCTTGCATTGCTCACTC+AGG | 0.569799 | 7.4:-18812988 | None:intergenic |
| ATGCTTCAGTTTCTGTACAG+TGG | 0.576559 | 7.4:-18813146 | MS.gene38766:CDS |
| AAGAGCAAGATCAACGGCTG+AGG | 0.588511 | 7.4:+18813046 | None:intergenic |
| CGGTCACCACAATTAGGCCT+AGG | 0.589003 | 7.4:+18813092 | None:intergenic |
| TCAACCCATCCGGTTCAGCT+AGG | 0.592108 | 7.4:-18813217 | MS.gene38766:CDS |
| CTAAGATGCATCTGTGTGCG+TGG | 0.593852 | 7.4:+18813318 | None:intergenic |
| GATGGGTCTAATCCAGAAGG+TGG | 0.598545 | 7.4:+18813251 | None:intergenic |
| AGTTAACTCAGTTGGTTACG+AGG | 0.607152 | 7.4:-18813180 | MS.gene38766:CDS |
| CCAGACCTAGCTGAACCGGA+TGG | 0.611566 | 7.4:+18813212 | None:intergenic |
| AGATCTTAGTGAGTCTTCAA+GGG | 0.612991 | 7.4:+18813421 | None:intergenic |
| CCTGATGGGTCTAATCCAGA+AGG | 0.623455 | 7.4:+18813248 | None:intergenic |
| CAGACCTAGCTGAACCGGAT+GGG | 0.623790 | 7.4:+18813213 | None:intergenic |
| CATGAACCTAGGCCTAATTG+TGG | 0.646688 | 7.4:-18813098 | MS.gene38766:CDS |
| ATGGGTCTAATCCAGAAGGT+GGG | 0.656727 | 7.4:+18813252 | None:intergenic |
| AGTATCAAGAGCAAGATCAA+CGG | 0.660445 | 7.4:+18813040 | None:intergenic |
| TTCAACTCCGAAGTATCTAG+CGG | 0.662542 | 7.4:+18813010 | None:intergenic |
| TTCCTCAGAAACATGAACCT+AGG | 0.668829 | 7.4:-18813109 | MS.gene38766:CDS |
| AATGTGTATGCCAACAACCA+CGG | 0.675672 | 7.4:+18813072 | None:intergenic |
| AGGCCTAATTGTGGTGACCG+TGG | 0.704495 | 7.4:-18813089 | MS.gene38766:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACTTAAACCTTCTCATCAA+CGG | - | chr7.4:18813028-18813047 | MS.gene38766:CDS | 30.0% | |
| TCTTCAGAAAATTCTTCTGT+GGG | - | chr7.4:18813134-18813153 | MS.gene38766:CDS | 30.0% | |
| TTCTTCAGAAAATTCTTCTG+TGG | - | chr7.4:18813133-18813152 | MS.gene38766:CDS | 30.0% | |
| AGATCTTAGTGAGTCTTCAA+GGG | + | chr7.4:18812996-18813015 | None:intergenic | 35.0% | |
| AGTATCAAGAGCAAGATCAA+CGG | + | chr7.4:18813377-18813396 | None:intergenic | 35.0% | |
| GTTATACCAGTTAACTCAGT+TGG | - | chr7.4:18813226-18813245 | MS.gene38766:CDS | 35.0% | |
| AATGTGTATGCCAACAACCA+CGG | + | chr7.4:18813345-18813364 | None:intergenic | 40.0% | |
| AGTTAACTCAGTTGGTTACG+AGG | - | chr7.4:18813234-18813253 | MS.gene38766:CDS | 40.0% | |
| ATGCTTCAGTTTCTGTACAG+TGG | - | chr7.4:18813268-18813287 | MS.gene38766:CDS | 40.0% | |
| GAGATCTTAGTGAGTCTTCA+AGG | + | chr7.4:18812997-18813016 | None:intergenic | 40.0% | |
| TCGTAACCAACTGAGTTAAC+TGG | + | chr7.4:18813235-18813254 | None:intergenic | 40.0% | |
| TTCCTCAGAAACATGAACCT+AGG | - | chr7.4:18813305-18813324 | MS.gene38766:CDS | 40.0% | |
| ! | TTCAACTCCGAAGTATCTAG+CGG | + | chr7.4:18813407-18813426 | None:intergenic | 40.0% |
| ATGGGTCTAATCCAGAAGGT+GGG | + | chr7.4:18813165-18813184 | None:intergenic | 45.0% | |
| CATGAACCTAGGCCTAATTG+TGG | - | chr7.4:18813316-18813335 | MS.gene38766:CDS | 45.0% | |
| AACACCAGACCTAGCTGAAC+CGG | + | chr7.4:18813209-18813228 | None:intergenic | 50.0% | |
| AAGAGCAAGATCAACGGCTG+AGG | + | chr7.4:18813371-18813390 | None:intergenic | 50.0% | |
| ATTGTGGTGACCGTGGTTGT+TGG | - | chr7.4:18813332-18813351 | MS.gene38766:CDS | 50.0% | |
| CAACCACGGTCACCACAATT+AGG | + | chr7.4:18813331-18813350 | None:intergenic | 50.0% | |
| CTAAGATGCATCTGTGTGCG+TGG | + | chr7.4:18813099-18813118 | None:intergenic | 50.0% | |
| CTTGCAGCCGCTAGATACTT+CGG | - | chr7.4:18813397-18813416 | MS.gene38766:CDS | 50.0% | |
| GATGGGTCTAATCCAGAAGG+TGG | + | chr7.4:18813166-18813185 | None:intergenic | 50.0% | |
| TCTAATCCAGAAGGTGGGTC+TGG | + | chr7.4:18813160-18813179 | None:intergenic | 50.0% | |
| ! | ACGCTTGTCCGTTGATGAGA+AGG | + | chr7.4:18813039-18813058 | None:intergenic | 50.0% |
| ! | CCTGATGGGTCTAATCCAGA+AGG | + | chr7.4:18813169-18813188 | None:intergenic | 50.0% |
| ! | CCTTCTGGATTAGACCCATC+AGG | - | chr7.4:18813166-18813185 | MS.gene38766:CDS | 50.0% |
| ! | GGCCTAGGTTCATGTTTCTG+AGG | + | chr7.4:18813310-18813329 | None:intergenic | 50.0% |
| !! | GACGTTGTTTTCAGCGTCGA+AGG | - | chr7.4:18813064-18813083 | MS.gene38766:CDS | 50.0% |
| !! | GGTTGACTCGGTTTCCTGAT+GGG | + | chr7.4:18813183-18813202 | None:intergenic | 50.0% |
| AGGCCTAATTGTGGTGACCG+TGG | - | chr7.4:18813325-18813344 | MS.gene38766:CDS | 55.0% | |
| CAGACCTAGCTGAACCGGAT+GGG | + | chr7.4:18813204-18813223 | None:intergenic | 55.0% | |
| CGGTCACCACAATTAGGCCT+AGG | + | chr7.4:18813325-18813344 | None:intergenic | 55.0% | |
| GGAAACCGAGTCAACCCATC+CGG | - | chr7.4:18813187-18813206 | MS.gene38766:CDS | 55.0% | |
| TCAACCCATCCGGTTCAGCT+AGG | - | chr7.4:18813197-18813216 | MS.gene38766:CDS | 55.0% | |
| !! | CTGAACCGGATGGGTTGACT+CGG | + | chr7.4:18813195-18813214 | None:intergenic | 55.0% |
| !! | GGGTTGACTCGGTTTCCTGA+TGG | + | chr7.4:18813184-18813203 | None:intergenic | 55.0% |
| CCAGACCTAGCTGAACCGGA+TGG | + | chr7.4:18813205-18813224 | None:intergenic | 60.0% | |
| CCATCCGGTTCAGCTAGGTC+TGG | - | chr7.4:18813202-18813221 | MS.gene38766:CDS | 60.0% | |
| TGTGGGCCAGACCCACCTTC+TGG | - | chr7.4:18813151-18813170 | MS.gene38766:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 18812989 | 18813447 | 18812989 | ID=MS.gene38766 |
| chr7.4 | mRNA | 18812989 | 18813447 | 18812989 | ID=MS.gene38766.t1;Parent=MS.gene38766 |
| chr7.4 | exon | 18812989 | 18813447 | 18812989 | ID=MS.gene38766.t1.exon1;Parent=MS.gene38766.t1 |
| chr7.4 | CDS | 18812989 | 18813447 | 18812989 | ID=cds.MS.gene38766.t1;Parent=MS.gene38766.t1 |
| Gene Sequence |
| Protein sequence |
