AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38845

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38845.t1 MTR_8g442410 96.082 536 21 0 6 541 1 536 0.0 1056
MS.gene38845.t1 MTR_4g104020 47.135 384 190 7 154 530 143 520 6.60e-104 322
MS.gene38845.t1 MTR_7g027190 41.562 397 195 6 154 530 294 673 5.60e-93 298
MS.gene38845.t1 MTR_3g065980 35.427 398 215 11 154 531 166 541 2.86e-76 251
MS.gene38845.t1 MTR_5g094450 34.625 387 230 7 156 530 157 532 1.07e-66 225
MS.gene38845.t1 MTR_3g089055 34.656 378 228 6 161 530 204 570 3.75e-66 224
MS.gene38845.t1 MTR_3g089055 34.656 378 228 6 161 530 199 565 4.11e-66 224
MS.gene38845.t1 MTR_5g097480 33.854 384 233 8 156 530 173 544 6.18e-65 221
MS.gene38845.t1 MTR_3g056110 32.812 384 237 7 156 530 171 542 2.79e-64 219
MS.gene38845.t1 MTR_1g029420 34.656 378 228 6 161 530 226 592 2.89e-64 220
MS.gene38845.t1 MTR_2g082090 35.806 391 216 9 156 530 208 579 1.39e-63 218
MS.gene38845.t1 MTR_4g133660 32.817 387 234 7 155 529 170 542 5.18e-58 202
MS.gene38845.t1 MTR_4g064150 30.467 407 257 7 140 532 341 735 7.61e-58 205
MS.gene38845.t1 MTR_7g074650 35.501 369 218 8 164 530 442 792 1.45e-57 206
MS.gene38845.t1 MTR_4g102790 32.732 388 241 11 152 529 284 661 3.27e-57 202
MS.gene38845.t1 MTR_4g064160 30.827 399 251 8 148 532 299 686 8.27e-56 199
MS.gene38845.t1 MTR_4g064180 31.510 384 248 6 152 527 246 622 3.53e-55 196
MS.gene38845.t1 MTR_4g064120 32.031 384 246 7 152 527 246 622 3.53e-55 196
MS.gene38845.t1 MTR_2g097410 31.726 394 239 7 154 532 365 743 3.98e-53 192
MS.gene38845.t1 MTR_4g064200 31.250 384 242 7 155 529 279 649 1.52e-52 189
MS.gene38845.t1 MTR_4g076020 35.000 380 213 11 164 538 88 438 4.82e-52 184
MS.gene38845.t1 MTR_2g097467 29.716 387 251 6 154 532 283 656 2.43e-51 186
MS.gene38845.t1 MTR_2g097310 28.916 415 256 11 134 532 249 640 9.07e-51 184
MS.gene38845.t1 MTR_2g097463 30.052 386 249 6 155 532 285 657 2.08e-50 184
MS.gene38845.t1 MTR_2g097473 29.199 387 253 6 154 532 283 656 2.95e-49 180
MS.gene38845.t1 MTR_2g026250 29.870 385 250 7 155 530 223 596 3.40e-49 179
MS.gene38845.t1 MTR_1g069725 33.590 390 219 11 152 529 101 462 9.01e-49 176
MS.gene38845.t1 MTR_7g062120 29.397 398 261 6 144 532 356 742 1.73e-48 179
MS.gene38845.t1 MTR_2g097390 29.442 394 236 10 155 529 270 640 6.76e-48 176
MS.gene38845.t1 MTR_2g097350 29.873 395 233 12 155 529 269 639 9.36e-48 176
MS.gene38845.t1 MTR_2g034250 31.362 389 250 10 153 530 203 585 1.28e-45 169
MS.gene38845.t1 MTR_2g034280 31.472 394 240 11 153 530 196 575 5.45e-45 167
MS.gene38845.t1 MTR_3g072710 33.007 409 230 15 152 533 112 503 1.26e-44 165
MS.gene38845.t1 MTR_3g022830 28.505 428 231 11 161 530 26 436 5.09e-44 162
MS.gene38845.t1 MTR_2g097380 28.496 379 216 9 165 532 229 563 1.43e-43 163
MS.gene38845.t1 MTR_5g058860 29.423 520 301 19 44 533 17 500 1.66e-43 162
MS.gene38845.t1 MTR_5g015490 28.272 382 256 7 161 530 115 490 2.72e-41 155
MS.gene38845.t1 MTR_2g034260 29.825 399 242 11 153 530 203 584 1.81e-40 154
MS.gene38845.t1 MTR_7g057230 31.858 339 206 9 200 529 334 656 7.53e-40 154
MS.gene38845.t1 MTR_4g097080 28.158 380 253 11 161 530 134 503 1.06e-39 151
MS.gene38845.t1 MTR_1g096030 29.369 412 230 17 154 530 74 459 1.89e-38 147
MS.gene38845.t1 MTR_1g086970 28.744 414 253 14 139 530 85 478 2.86e-38 147
MS.gene38845.t1 MTR_4g076140 45.270 148 79 1 385 530 322 469 1.78e-37 144
MS.gene38845.t1 MTR_4g076140 34.222 225 126 7 153 372 41 248 1.01e-26 113
MS.gene38845.t1 MTR_5g009080 43.421 152 84 1 382 531 328 479 1.20e-36 142
MS.gene38845.t1 MTR_5g009080 32.456 228 126 7 153 372 49 256 7.12e-26 111
MS.gene38845.t1 MTR_4g077760 28.831 385 244 10 157 530 190 555 8.56e-35 138
MS.gene38845.t1 MTR_6g047750 30.848 389 232 14 159 530 294 662 3.89e-34 137
MS.gene38845.t1 MTR_4g095500 27.404 416 247 12 161 529 60 467 7.42e-34 134
MS.gene38845.t1 MTR_5g015950 26.859 417 250 14 161 529 36 445 1.15e-32 130
MS.gene38845.t1 MTR_3g053270 26.859 417 250 14 161 529 36 445 1.15e-32 130
MS.gene38845.t1 MTR_2g099110 28.462 390 246 10 161 530 75 451 1.17e-31 127
MS.gene38845.t1 MTR_2g089100 27.446 419 255 9 146 529 38 442 3.98e-31 126
MS.gene38845.t1 MTR_7g109580 25.773 388 265 12 152 530 181 554 2.98e-27 115
MS.gene38845.t1 MTR_8g093070 26.329 395 256 13 157 540 136 506 1.24e-26 113
MS.gene38845.t1 MTR_0092s0100 24.352 386 254 10 157 530 372 731 8.62e-22 100
MS.gene38845.t1 MTR_0092s0100 24.352 386 254 10 157 530 369 728 8.89e-22 99.8
MS.gene38845.t1 MTR_4g026485 22.922 397 263 12 147 530 258 624 1.41e-17 86.7
MS.gene38845.t1 MTR_8g020840 22.362 398 262 13 161 531 172 549 1.42e-16 83.2
MS.gene38845.t1 MTR_3g022580 35.385 130 74 4 409 530 56 183 1.44e-15 75.5
MS.gene38845.t1 MTR_8g077940 23.469 392 255 15 156 530 178 541 1.09e-14 77.0
MS.gene38845.t1 MTR_3g022005 37.634 93 55 2 440 530 34 125 1.01e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38845.t1 AT1G66350 38.120 383 208 11 154 531 149 507 1.34e-77 253
MS.gene38845.t1 AT3G03450 37.662 385 220 10 154 532 177 547 5.81e-76 250
MS.gene38845.t1 AT1G14920 37.726 387 214 11 154 533 166 532 2.15e-74 246
MS.gene38845.t1 AT2G01570 36.667 390 220 11 154 536 218 587 2.48e-73 244
MS.gene38845.t1 AT5G17490 31.568 491 291 12 69 533 48 519 7.22e-68 228
MS.gene38845.t1 AT2G04890 34.115 384 225 7 156 530 49 413 1.93e-63 213
MS.gene38845.t1 AT5G48150 33.420 383 237 6 156 530 118 490 2.33e-63 215
MS.gene38845.t1 AT5G48150 33.420 383 237 6 156 530 118 490 2.33e-63 215
MS.gene38845.t1 AT5G48150 33.420 383 237 6 156 530 118 490 2.33e-63 215
MS.gene38845.t1 AT5G48150 33.420 383 237 6 156 530 118 490 2.33e-63 215
MS.gene38845.t1 AT3G54220 37.197 371 210 8 164 529 297 649 2.47e-61 214
MS.gene38845.t1 AT1G50600 33.594 384 234 7 156 530 155 526 1.40e-60 209
MS.gene38845.t1 AT1G21450 32.895 380 237 5 159 530 224 593 2.47e-60 209
MS.gene38845.t1 AT1G50600 33.594 384 234 7 156 530 226 597 7.72e-60 208
MS.gene38845.t1 AT1G50600 33.594 384 234 7 156 530 230 601 8.92e-60 208
MS.gene38845.t1 AT5G41920 35.000 380 228 9 154 530 37 400 1.85e-57 197
MS.gene38845.t1 AT1G07530 28.571 434 288 8 110 533 347 768 9.09e-56 200
MS.gene38845.t1 AT2G29060 31.592 402 252 7 140 529 264 654 8.73e-55 196
MS.gene38845.t1 AT2G29060 31.592 402 252 7 140 529 301 691 1.93e-54 195
MS.gene38845.t1 AT4G17230 32.824 393 239 8 149 530 147 525 6.93e-54 191
MS.gene38845.t1 AT4G17230 32.824 393 239 8 149 530 147 525 6.93e-54 191
MS.gene38845.t1 AT2G29065 27.216 485 320 12 65 529 156 627 8.71e-51 184
MS.gene38845.t1 AT1G07520 27.074 458 292 9 85 529 100 528 2.38e-49 178
MS.gene38845.t1 AT1G07520 27.074 458 292 9 85 529 100 528 2.38e-49 178
MS.gene38845.t1 AT5G59450 28.406 433 283 10 111 532 184 600 5.21e-49 179
MS.gene38845.t1 AT1G07520 27.074 458 292 9 85 529 265 693 1.21e-48 179
MS.gene38845.t1 AT1G07520 27.074 458 292 9 85 529 264 692 1.27e-48 179
MS.gene38845.t1 AT1G55580 32.086 374 217 10 191 530 75 445 3.80e-47 171
MS.gene38845.t1 AT3G46600 29.008 393 252 8 152 532 204 581 5.67e-47 173
MS.gene38845.t1 AT3G46600 29.008 393 252 8 152 532 172 549 1.24e-46 171
MS.gene38845.t1 AT3G46600 29.091 385 246 8 161 533 5 374 1.40e-46 167
MS.gene38845.t1 AT1G63100 30.447 358 233 7 151 503 243 589 1.64e-46 172
MS.gene38845.t1 AT1G63100 30.447 358 233 7 151 503 268 614 1.76e-46 172
MS.gene38845.t1 AT2G37650 28.753 393 258 7 150 533 325 704 1.88e-45 170
MS.gene38845.t1 AT2G37650 28.753 393 258 7 150 533 337 716 1.91e-45 171
MS.gene38845.t1 AT4G37650 27.561 410 271 12 141 530 126 529 6.83e-42 158
MS.gene38845.t1 AT5G66770 29.534 386 242 10 157 530 217 584 1.06e-39 152
MS.gene38845.t1 AT3G50650 30.055 366 223 9 180 530 195 542 8.11e-39 149
MS.gene38845.t1 AT5G52510 31.548 336 205 8 217 530 308 640 5.12e-38 148
MS.gene38845.t1 AT4G08250 29.231 390 252 12 152 529 99 476 6.31e-38 146
MS.gene38845.t1 AT1G50420 43.137 153 85 1 380 530 327 479 7.17e-35 137
MS.gene38845.t1 AT1G50420 32.444 225 138 4 153 375 50 262 8.45e-29 120
MS.gene38845.t1 AT3G49950 27.708 397 239 15 161 529 31 407 5.12e-31 125
MS.gene38845.t1 AT3G60630 26.710 307 201 10 238 530 326 622 8.48e-22 99.8
MS.gene38845.t1 AT4G00150 26.292 445 286 16 99 533 145 557 1.00e-18 90.1
MS.gene38845.t1 AT2G45160 24.731 465 282 19 99 530 210 639 1.42e-18 89.7
MS.gene38845.t1 AT4G36710 23.018 391 267 12 152 530 117 485 1.73e-17 85.9
MS.gene38845.t1 AT3G13840 23.881 402 256 15 150 530 138 510 5.88e-17 84.3

Find 101 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAAATGAACGGCAATTT+TGG 0.207673 8.4:-52238905 MS.gene38845:CDS
CTCAATACACATAATTGATT+TGG 0.221010 8.4:-52238489 MS.gene38845:CDS
CACAATGGACCTTTCTTTCT+TGG 0.232793 8.4:-52238086 MS.gene38845:CDS
GCAGGGCAAAACCTTCTAAT+AGG 0.248061 8.4:+52239241 None:intergenic
ATCCTAATTGGACTATTTCT+TGG 0.252505 8.4:+52238002 None:intergenic
TGATCTTGTTCCATTCTTGC+AGG 0.253249 8.4:+52239223 None:intergenic
TTCCTTTCCAACCAAGTAAA+AGG 0.254662 8.4:+52237763 None:intergenic
TTTGTTACTTAGTAAAATTT+TGG 0.269956 8.4:-52238771 MS.gene38845:CDS
TTCAATGGCCTTCGCTAATA+AGG 0.276663 8.4:-52238451 MS.gene38845:CDS
ACAACACCTTATATTACTTT+TGG 0.300835 8.4:-52238560 MS.gene38845:CDS
GATCAATGGAGAAGACAATT+AGG 0.324260 8.4:-52237891 MS.gene38845:CDS
TCATGTCTCTCCATTCTATC+AGG 0.340101 8.4:+52237921 None:intergenic
ATGATGTTTCTTTGATTACT+AGG 0.342089 8.4:-52238619 MS.gene38845:CDS
GACACTTTCATTCATATAGA+AGG 0.344185 8.4:+52239276 None:intergenic
TTTGGCTTTGTCAAGTTCTT+CGG 0.351298 8.4:-52238753 MS.gene38845:CDS
ATGAAACCAAAAGTAATATA+AGG 0.362911 8.4:+52238554 None:intergenic
TTGGCTTTGTCAAGTTCTTC+GGG 0.364499 8.4:-52238752 MS.gene38845:CDS
CCTCCAAAGCTCAGGATAAC+AGG 0.367635 8.4:-52238404 MS.gene38845:CDS
TCAATACACATAATTGATTT+GGG 0.373143 8.4:-52238488 MS.gene38845:CDS
CCTGTTATCCTGAGCTTTGG+AGG 0.384703 8.4:+52238404 None:intergenic
ACCTTGATTTCCCTATTAGA+AGG 0.391553 8.4:-52239252 MS.gene38845:CDS
TTGGTGAATGATGATGTCTT+TGG 0.396171 8.4:-52239004 MS.gene38845:CDS
CCTGAGCTTTGGAGGCCCCT+CGG 0.396208 8.4:+52238412 None:intergenic
TGACTTGGTCCATACGCTAC+TGG 0.405114 8.4:-52238837 MS.gene38845:CDS
TGCCAAGAAATAGTCCAATT+AGG 0.408355 8.4:-52238004 MS.gene38845:CDS
CTGAGCTTTGGAGGCCCCTC+GGG 0.412847 8.4:+52238413 None:intergenic
TCGACAACTGTGATTGCAAT+TGG 0.413117 8.4:+52238122 None:intergenic
ATATACATTGTCATGTGAAA+AGG 0.427162 8.4:-52237790 MS.gene38845:CDS
CAGGGCAAAACCTTCTAATA+GGG 0.438270 8.4:+52239242 None:intergenic
AAGTAATATAAGGTGTTGTT+TGG 0.439313 8.4:+52238564 None:intergenic
AATCCTGTTATCCTGAGCTT+TGG 0.439914 8.4:+52238401 None:intergenic
GTAAGAAGAGAAGGCGTTGC+CGG 0.440003 8.4:+52238272 None:intergenic
TGTAGAGGAAGCACTCTCAT+TGG 0.457377 8.4:-52238327 MS.gene38845:CDS
GACATGAAAGAGTAGATCAA+TGG 0.459154 8.4:-52237905 MS.gene38845:CDS
ATGGACCTTTCTTTCTTGGT+AGG 0.464231 8.4:-52238082 MS.gene38845:CDS
GATCTTGTTCCATTCTTGCA+GGG 0.465440 8.4:+52239224 None:intergenic
GCTATATGCCAAGGATCAAA+AGG 0.466005 8.4:-52238518 MS.gene38845:CDS
GAAAGGAAGGTCTATTATGA+TGG 0.467802 8.4:-52237748 MS.gene38845:CDS
ACCTTCTAATAGGGAAATCA+AGG 0.468486 8.4:+52239251 None:intergenic
GTAGAGGAAGCACTCTCATT+GGG 0.469254 8.4:-52238326 MS.gene38845:CDS
CTGGCTTGTGCCGAGGCCGT+TGG 0.479503 8.4:-52238818 MS.gene38845:CDS
TGATGTTTCTTTGATTACTA+GGG 0.480820 8.4:-52238618 MS.gene38845:CDS
TCTTCATCCTCCATTGTGGT+TGG 0.481380 8.4:+52238974 None:intergenic
GCCTTGGTTCATACCGAGAC+TGG 0.481981 8.4:+52238862 None:intergenic
GCCCCTCGGGCCTCGAAGCA+AGG 0.482725 8.4:+52238426 None:intergenic
GATTACTAGGGAGAATAAGT+TGG 0.489189 8.4:-52238606 MS.gene38845:CDS
CCGAGGGGCCTCCAAAGCTC+AGG 0.495752 8.4:-52238412 MS.gene38845:CDS
CATCATCAAGAACTGTCTCT+TGG 0.496418 8.4:+52239182 None:intergenic
TTGCGACGGCATTGAAGTGT+AGG 0.497490 8.4:-52238700 MS.gene38845:CDS
GTCTCTGCAACCAACGGCCT+CGG 0.503923 8.4:+52238808 None:intergenic
AGAAGACAATTAGGAAGAGC+AGG 0.503981 8.4:-52237882 MS.gene38845:CDS
CTGATATTATGCGAAATTCA+AGG 0.506362 8.4:+52238297 None:intergenic
CCGAGACTGGCTTGTGATTG+TGG 0.515572 8.4:+52238875 None:intergenic
CTTTCTGTTTATGATTGTGA+TGG 0.518538 8.4:-52237813 MS.gene38845:CDS
CTATTATGATGGCTTCTGCT+TGG 0.519258 8.4:-52237737 MS.gene38845:CDS
CGGCACAAGCCAGTAGCGTA+TGG 0.524974 8.4:+52238828 None:intergenic
TAATAAGGAACCTTGCTTCG+AGG 0.526138 8.4:-52238436 MS.gene38845:CDS
ATTCACCTTCTCTCAAGTTG+AGG 0.528790 8.4:+52238240 None:intergenic
TATGGACCAAGTCAAGGCCT+TGG 0.530054 8.4:+52238846 None:intergenic
AACCTTGCTTCGAGGCCCGA+GGG 0.530262 8.4:-52238428 MS.gene38845:CDS
ACACATAATTGATTTGGGAA+TGG 0.530660 8.4:-52238483 MS.gene38845:CDS
AAGTCATCAACTTCTGAAGA+AGG 0.531009 8.4:+52239115 None:intergenic
AGAAACCTACCAAGAAAGAA+AGG 0.532154 8.4:+52238077 None:intergenic
TTGTGAGGGTCCTGATAGAA+TGG 0.538774 8.4:-52237931 MS.gene38845:CDS
TATACATTGTCATGTGAAAA+GGG 0.539789 8.4:-52237789 MS.gene38845:CDS
TTGCAACATTGTAGCTTGTG+AGG 0.544201 8.4:-52237946 MS.gene38845:CDS
CAAGAACTGTCTCTTGGTCA+TGG 0.547218 8.4:+52239188 None:intergenic
AAATTCTTCATCCTCCATTG+TGG 0.547730 8.4:+52238970 None:intergenic
TGCAACATTGTAGCTTGTGA+GGG 0.548434 8.4:-52237945 MS.gene38845:CDS
GAATGGAGCATACTCTTCAA+TGG 0.549472 8.4:-52238466 MS.gene38845:CDS
ATTTCGCATAATATCAGAGC+CGG 0.550299 8.4:-52238291 MS.gene38845:CDS
TGTTGATAGCTTCATCAACA+TGG 0.554822 8.4:-52239092 MS.gene38845:CDS
GAACCTTGCTTCGAGGCCCG+AGG 0.559070 8.4:-52238429 MS.gene38845:CDS
TTCTCGATTGTAAGAAGAGA+AGG 0.560917 8.4:+52238263 None:intergenic
TAGCATGAATTTGCTTGTAG+AGG 0.564272 8.4:-52238342 MS.gene38845:CDS
TACGCTACTGGCTTGTGCCG+AGG 0.566964 8.4:-52238825 MS.gene38845:CDS
TGGTTGCAGAGACAACAATC+AGG 0.574071 8.4:-52238798 MS.gene38845:CDS
GACGAAGAAGTTGAAATGAA+CGG 0.577431 8.4:-52238914 MS.gene38845:CDS
GCTTGACTTGTACACTTGAG+TGG 0.583171 8.4:+52237846 None:intergenic
GCAAGGTTCCTTATTAGCGA+AGG 0.585586 8.4:+52238443 None:intergenic
TATGAACCAAGGCCTTGACT+TGG 0.587577 8.4:-52238852 MS.gene38845:CDS
CCACAATCACAAGCCAGTCT+CGG 0.590657 8.4:-52238875 MS.gene38845:CDS
AGGTAATAAACAAGGAGTGG+TGG 0.593777 8.4:+52239135 None:intergenic
CTATTTGAAAATACATTATG+CGG 0.595538 8.4:+52238665 None:intergenic
CAATGCCGTCGCAAAACAGT+AGG 0.603505 8.4:+52238709 None:intergenic
GATGGCTTCTGCTTGGCAAG+TGG 0.608972 8.4:-52237730 MS.gene38845:CDS
GAGAACCTCAACTTGAGAGA+AGG 0.614360 8.4:-52238245 MS.gene38845:CDS
GCAAATGAAGCTATATGCCA+AGG 0.622841 8.4:-52238527 MS.gene38845:CDS
ATTGTTGTCTCTGCAACCAA+CGG 0.634384 8.4:+52238802 None:intergenic
GCCAGTCTCGGTATGAACCA+AGG 0.638041 8.4:-52238863 MS.gene38845:CDS
GTAGCGTATGGACCAAGTCA+AGG 0.641162 8.4:+52238840 None:intergenic
CAATGGAAGACTTAAACATG+TGG 0.648915 8.4:-52239303 MS.gene38845:CDS
ACCTTGCTTCGAGGCCCGAG+GGG 0.649136 8.4:-52238427 MS.gene38845:CDS
AGAAGGTAATAAACAAGGAG+TGG 0.651035 8.4:+52239132 None:intergenic
GAGCAAGACACGAACCACAA+TGG 0.663179 8.4:-52238101 MS.gene38845:CDS
TGGATATGATCCAACCACAA+TGG 0.666133 8.4:-52238984 MS.gene38845:CDS
AAATATGTGAAAGAAAGTAG+AGG 0.666936 8.4:-52238185 MS.gene38845:CDS
TCTGAAGAAGGTAATAAACA+AGG 0.670330 8.4:+52239127 None:intergenic
ATATGATCCAACCACAATGG+AGG 0.672820 8.4:-52238981 MS.gene38845:CDS
AGAATGGAACAAGATCAAGA+AGG 0.680580 8.4:-52239217 MS.gene38845:CDS
CAAGAAAGAAAGGTCCATTG+TGG 0.695513 8.4:+52238087 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTACTTAGTAAAATTT+TGG - chr8.4:52238228-52238247 MS.gene38845:CDS 15.0%
!! ATGAAACCAAAAGTAATATA+AGG + chr8.4:52238448-52238467 None:intergenic 20.0%
!! CTATTTGAAAATACATTATG+CGG + chr8.4:52238337-52238356 None:intergenic 20.0%
!!! AAATTTTGATCATCACTTTT+TGG - chr8.4:52237976-52237995 MS.gene38845:CDS 20.0%
!!! TCAATACACATAATTGATTT+GGG - chr8.4:52238511-52238530 MS.gene38845:CDS 20.0%
!!! TTATATTACTTTTGGTTTCA+TGG - chr8.4:52238447-52238466 MS.gene38845:CDS 20.0%
! AAATATGTGAAAGAAAGTAG+AGG - chr8.4:52238814-52238833 MS.gene38845:CDS 25.0%
! ATATACATTGTCATGTGAAA+AGG - chr8.4:52239209-52239228 MS.gene38845:CDS 25.0%
! ATGATGTTTCTTTGATTACT+AGG - chr8.4:52238380-52238399 MS.gene38845:CDS 25.0%
! CTCAATACACATAATTGATT+TGG - chr8.4:52238510-52238529 MS.gene38845:CDS 25.0%
! TATACATTGTCATGTGAAAA+GGG - chr8.4:52239210-52239229 MS.gene38845:CDS 25.0%
! TGATGTTTCTTTGATTACTA+GGG - chr8.4:52238381-52238400 MS.gene38845:CDS 25.0%
!! ACAACACCTTATATTACTTT+TGG - chr8.4:52238439-52238458 MS.gene38845:CDS 25.0%
!!! AAGTAATATAAGGTGTTGTT+TGG + chr8.4:52238438-52238457 None:intergenic 25.0%
CTGATATTATGCGAAATTCA+AGG + chr8.4:52238705-52238724 None:intergenic 30.0%
CTTTCTGTTTATGATTGTGA+TGG - chr8.4:52239186-52239205 MS.gene38845:CDS 30.0%
GACACTTTCATTCATATAGA+AGG + chr8.4:52237726-52237745 None:intergenic 30.0%
TCTGAAGAAGGTAATAAACA+AGG + chr8.4:52237875-52237894 None:intergenic 30.0%
! ATCCTAATTGGACTATTTCT+TGG + chr8.4:52239000-52239019 None:intergenic 30.0%
! CTCTCTATTTTCATCCTAAT+TGG + chr8.4:52239012-52239031 None:intergenic 30.0%
!! ACACATAATTGATTTGGGAA+TGG - chr8.4:52238516-52238535 MS.gene38845:CDS 30.0%
AAATTCTTCATCCTCCATTG+TGG + chr8.4:52238032-52238051 None:intergenic 35.0%
ACCTTCTAATAGGGAAATCA+AGG + chr8.4:52237751-52237770 None:intergenic 35.0%
ACCTTGATTTCCCTATTAGA+AGG - chr8.4:52237747-52237766 MS.gene38845:CDS 35.0%
AGAAACCTACCAAGAAAGAA+AGG + chr8.4:52238925-52238944 None:intergenic 35.0%
AGAAGGTAATAAACAAGGAG+TGG + chr8.4:52237870-52237889 None:intergenic 35.0%
AGAATGGAACAAGATCAAGA+AGG - chr8.4:52237782-52237801 MS.gene38845:CDS 35.0%
ATTTCGCATAATATCAGAGC+CGG - chr8.4:52238708-52238727 MS.gene38845:CDS 35.0%
GAAAGGAAGGTCTATTATGA+TGG - chr8.4:52239251-52239270 MS.gene38845:CDS 35.0%
GACATGAAAGAGTAGATCAA+TGG - chr8.4:52239094-52239113 MS.gene38845:CDS 35.0%
GACGAAGAAGTTGAAATGAA+CGG - chr8.4:52238085-52238104 MS.gene38845:CDS 35.0%
GATCAATGGAGAAGACAATT+AGG - chr8.4:52239108-52239127 MS.gene38845:CDS 35.0%
GATTACTAGGGAGAATAAGT+TGG - chr8.4:52238393-52238412 MS.gene38845:CDS 35.0%
TGCCAAGAAATAGTCCAATT+AGG - chr8.4:52238995-52239014 MS.gene38845:CDS 35.0%
TTCCTTTCCAACCAAGTAAA+AGG + chr8.4:52239239-52239258 None:intergenic 35.0%
TTCTCGATTGTAAGAAGAGA+AGG + chr8.4:52238739-52238758 None:intergenic 35.0%
! AAGTCATCAACTTCTGAAGA+AGG + chr8.4:52237887-52237906 None:intergenic 35.0%
! GTTGAAATGAACGGCAATTT+TGG - chr8.4:52238094-52238113 MS.gene38845:CDS 35.0%
! TAGCATGAATTTGCTTGTAG+AGG - chr8.4:52238657-52238676 MS.gene38845:CDS 35.0%
! TTGGTGAATGATGATGTCTT+TGG - chr8.4:52237995-52238014 MS.gene38845:CDS 35.0%
! TTTTACTTGGTTGGAAAGGA+AGG - chr8.4:52239238-52239257 MS.gene38845:CDS 35.0%
!! AGCTTTTTCCTTTTGATCCT+TGG + chr8.4:52238492-52238511 None:intergenic 35.0%
!! TGTTGATAGCTTCATCAACA+TGG - chr8.4:52237907-52237926 MS.gene38845:CDS 35.0%
!! TTTGGCTTTGTCAAGTTCTT+CGG - chr8.4:52238246-52238265 MS.gene38845:CDS 35.0%
AATCCTGTTATCCTGAGCTT+TGG + chr8.4:52238601-52238620 None:intergenic 40.0%
AGAAGACAATTAGGAAGAGC+AGG - chr8.4:52239117-52239136 MS.gene38845:CDS 40.0%
AGGTAATAAACAAGGAGTGG+TGG + chr8.4:52237867-52237886 None:intergenic 40.0%
ATATGATCCAACCACAATGG+AGG - chr8.4:52238018-52238037 MS.gene38845:CDS 40.0%
ATGGACCTTTCTTTCTTGGT+AGG - chr8.4:52238917-52238936 MS.gene38845:CDS 40.0%
ATTGTTGTCTCTGCAACCAA+CGG + chr8.4:52238200-52238219 None:intergenic 40.0%
CAAGAAAGAAAGGTCCATTG+TGG + chr8.4:52238915-52238934 None:intergenic 40.0%
CACAATGGACCTTTCTTTCT+TGG - chr8.4:52238913-52238932 MS.gene38845:CDS 40.0%
CAGGGCAAAACCTTCTAATA+GGG + chr8.4:52237760-52237779 None:intergenic 40.0%
CATCATCAAGAACTGTCTCT+TGG + chr8.4:52237820-52237839 None:intergenic 40.0%
CTATTATGATGGCTTCTGCT+TGG - chr8.4:52239262-52239281 MS.gene38845:CDS 40.0%
GAATGGAGCATACTCTTCAA+TGG - chr8.4:52238533-52238552 MS.gene38845:CDS 40.0%
GATCTTGTTCCATTCTTGCA+GGG + chr8.4:52237778-52237797 None:intergenic 40.0%
GCAAATGAAGCTATATGCCA+AGG - chr8.4:52238472-52238491 MS.gene38845:CDS 40.0%
GCTATATGCCAAGGATCAAA+AGG - chr8.4:52238481-52238500 MS.gene38845:CDS 40.0%
TAATAAGGAACCTTGCTTCG+AGG - chr8.4:52238563-52238582 MS.gene38845:CDS 40.0%
TCATGTCTCTCCATTCTATC+AGG + chr8.4:52239081-52239100 None:intergenic 40.0%
TCGACAACTGTGATTGCAAT+TGG + chr8.4:52238880-52238899 None:intergenic 40.0%
TGATCTTGTTCCATTCTTGC+AGG + chr8.4:52237779-52237798 None:intergenic 40.0%
TGGATATGATCCAACCACAA+TGG - chr8.4:52238015-52238034 MS.gene38845:CDS 40.0%
TTCAATGGCCTTCGCTAATA+AGG - chr8.4:52238548-52238567 MS.gene38845:CDS 40.0%
! ATTCACCTTCTCTCAAGTTG+AGG + chr8.4:52238762-52238781 None:intergenic 40.0%
! GAAAAGGGTTGCCTTTTACT+TGG - chr8.4:52239225-52239244 MS.gene38845:CDS 40.0%
! TGCAACATTGTAGCTTGTGA+GGG - chr8.4:52239054-52239073 MS.gene38845:CDS 40.0%
! TGCCTTTTACTTGGTTGGAA+AGG - chr8.4:52239234-52239253 MS.gene38845:CDS 40.0%
! TTGCAACATTGTAGCTTGTG+AGG - chr8.4:52239053-52239072 MS.gene38845:CDS 40.0%
!! TTGGCTTTGTCAAGTTCTTC+GGG - chr8.4:52238247-52238266 MS.gene38845:CDS 40.0%
CAAGAACTGTCTCTTGGTCA+TGG + chr8.4:52237814-52237833 None:intergenic 45.0%
GAGAACCTCAACTTGAGAGA+AGG - chr8.4:52238754-52238773 MS.gene38845:CDS 45.0%
GCAAGGTTCCTTATTAGCGA+AGG + chr8.4:52238559-52238578 None:intergenic 45.0%
GCAGGGCAAAACCTTCTAAT+AGG + chr8.4:52237761-52237780 None:intergenic 45.0%
TATGAACCAAGGCCTTGACT+TGG - chr8.4:52238147-52238166 MS.gene38845:CDS 45.0%
TCTTCATCCTCCATTGTGGT+TGG + chr8.4:52238028-52238047 None:intergenic 45.0%
TGGTTGCAGAGACAACAATC+AGG - chr8.4:52238201-52238220 MS.gene38845:CDS 45.0%
TTGTGAGGGTCCTGATAGAA+TGG - chr8.4:52239068-52239087 MS.gene38845:CDS 45.0%
! AAGGTTTTGCCCTGCAAGAA+TGG - chr8.4:52237766-52237785 MS.gene38845:CDS 45.0%
! AGGGTTGCCTTTTACTTGGT+TGG - chr8.4:52239229-52239248 MS.gene38845:CDS 45.0%
! AGTCTCCTACTGTTTTGCGA+CGG - chr8.4:52238285-52238304 MS.gene38845:CDS 45.0%
!! GCTTGACTTGTACACTTGAG+TGG + chr8.4:52239156-52239175 None:intergenic 45.0%
!! GTAGAGGAAGCACTCTCATT+GGG - chr8.4:52238673-52238692 MS.gene38845:CDS 45.0%
!! TGTAGAGGAAGCACTCTCAT+TGG - chr8.4:52238672-52238691 MS.gene38845:CDS 45.0%
CAATGCCGTCGCAAAACAGT+AGG + chr8.4:52238293-52238312 None:intergenic 50.0%
CCACAATCACAAGCCAGTCT+CGG - chr8.4:52238124-52238143 MS.gene38845:CDS 50.0%
CCTCCAAAGCTCAGGATAAC+AGG - chr8.4:52238595-52238614 MS.gene38845:CDS 50.0%
GAGCAAGACACGAACCACAA+TGG - chr8.4:52238898-52238917 MS.gene38845:CDS 50.0%
GTAGCGTATGGACCAAGTCA+AGG + chr8.4:52238162-52238181 None:intergenic 50.0%
TATGGACCAAGTCAAGGCCT+TGG + chr8.4:52238156-52238175 None:intergenic 50.0%
! CCTGTTATCCTGAGCTTTGG+AGG + chr8.4:52238598-52238617 None:intergenic 50.0%
! TTGCGACGGCATTGAAGTGT+AGG - chr8.4:52238299-52238318 MS.gene38845:CDS 50.0%
!! GTAAGAAGAGAAGGCGTTGC+CGG + chr8.4:52238730-52238749 None:intergenic 50.0%
!! TGACTTGGTCCATACGCTAC+TGG - chr8.4:52238162-52238181 MS.gene38845:CDS 50.0%
GATGGCTTCTGCTTGGCAAG+TGG - chr8.4:52239269-52239288 MS.gene38845:CDS 55.0%
GCCAGTCTCGGTATGAACCA+AGG - chr8.4:52238136-52238155 MS.gene38845:CDS 55.0%
GCCTTGGTTCATACCGAGAC+TGG + chr8.4:52238140-52238159 None:intergenic 55.0%
!! CCGAGACTGGCTTGTGATTG+TGG + chr8.4:52238127-52238146 None:intergenic 55.0%
AACCTTGCTTCGAGGCCCGA+GGG - chr8.4:52238571-52238590 MS.gene38845:CDS 60.0%
CGGCACAAGCCAGTAGCGTA+TGG + chr8.4:52238174-52238193 None:intergenic 60.0%
GTCTCTGCAACCAACGGCCT+CGG + chr8.4:52238194-52238213 None:intergenic 60.0%
!! TACGCTACTGGCTTGTGCCG+AGG - chr8.4:52238174-52238193 MS.gene38845:CDS 60.0%
ACCTTGCTTCGAGGCCCGAG+GGG - chr8.4:52238572-52238591 MS.gene38845:CDS 65.0%
GAACCTTGCTTCGAGGCCCG+AGG - chr8.4:52238570-52238589 MS.gene38845:CDS 65.0%
!! CCTGAGCTTTGGAGGCCCCT+CGG + chr8.4:52238590-52238609 None:intergenic 65.0%
!! CTGAGCTTTGGAGGCCCCTC+GGG + chr8.4:52238589-52238608 None:intergenic 65.0%
CCGAGGGGCCTCCAAAGCTC+AGG - chr8.4:52238587-52238606 MS.gene38845:CDS 70.0%
! CTGGCTTGTGCCGAGGCCGT+TGG - chr8.4:52238181-52238200 MS.gene38845:CDS 70.0%
GCCCCTCGGGCCTCGAAGCA+AGG + chr8.4:52238576-52238595 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 52237698 52239323 52237698 ID=MS.gene38845
chr8.4 mRNA 52237698 52239323 52237698 ID=MS.gene38845.t1;Parent=MS.gene38845
chr8.4 exon 52237698 52239323 52237698 ID=MS.gene38845.t1.exon1;Parent=MS.gene38845.t1
chr8.4 CDS 52237698 52239323 52237698 ID=cds.MS.gene38845.t1;Parent=MS.gene38845.t1
Gene Sequence

>MS.gene38845

ATGGAAGACTTAAACATGTGGCTTTCCTTCTATATGAATGAAAGTGTCAACCTTGATTTCCCTATTAGAAGGTTTTGCCCTGCAAGAATGGAACAAGATCAAGAAGGAGATTGCCATGACCAAGAGACAGTTCTTGATGATGTTGAATTGTGTACTTTTGATACTACCACCACTCCTTGTTTATTACCTTCTTCAGAAGTTGATGACTTTGTTGATAGCTTCATCAACATGGATCAATATGAAGATCAAGAGTCTCAAGAGAAGAATCATCAAAGTCAAAATTTTGATCATCACTTTTTGGTGAATGATGATGTCTTTGGATATGATCCAACCACAATGGAGGATGAAGAATTTGAGATCTGCGAAAATACAACAATCACAAATGAAGACGAAGAAGTTGAAATGAACGGCAATTTTGGTGCTATTCCACAATCACAAGCCAGTCTCGGTATGAACCAAGGCCTTGACTTGGTCCATACGCTACTGGCTTGTGCCGAGGCCGTTGGTTGCAGAGACAACAATCAGGCAAATTTGTTACTTAGTAAAATTTTGGCTTTGTCAAGTTCTTCGGGAGATTCATTACAAAGAGTCTCCTACTGTTTTGCGACGGCATTGAAGTGTAGGTTATCACTTTATCCGCATAATGTATTTTCAAATAGTACACTCACAACAAGTACTTCTAATGATGTTTCTTTGATTACTAGGGAGAATAAGTTGGAAGCTTTTCAACTTCTTTACCAAACAACACCTTATATTACTTTTGGTTTCATGGCTGCAAATGAAGCTATATGCCAAGGATCAAAAGGAAAAAGCTCAATACACATAATTGATTTGGGAATGGAGCATACTCTTCAATGGCCTTCGCTAATAAGGAACCTTGCTTCGAGGCCCGAGGGGCCTCCAAAGCTCAGGATAACAGGATTCAGTACGAATGAAGAAAACAACGCAAAACTTCGCGCTAGCATGAATTTGCTTGTAGAGGAAGCACTCTCATTGGGAATAGTCCTTGAATTTCGCATAATATCAGAGCCGGCAACGCCTTCTCTTCTTACAATCGAGAACCTCAACTTGAGAGAAGGTGAATCTTTATTCGTTAATAGCATCTTGAAATTGCACAAATATGTGAAAGAAAGTAGAGGCTATCTAAAATCAATTCTTCAGTCAATCAAGAAGCTAAGTCCAATTGCAATCACAGTTGTCGAGCAAGACACGAACCACAATGGACCTTTCTTTCTTGGTAGGTTTCTAGAATCACTTCATTATTACTCAGCAATATTTGATTCTCTTGAAGCAAGTATGCCAAGAAATAGTCCAATTAGGATGAAAATAGAGAGAGTACATTTTGCAGAAGAAATTTGCAACATTGTAGCTTGTGAGGGTCCTGATAGAATGGAGAGACATGAAAGAGTAGATCAATGGAGAAGACAATTAGGAAGAGCAGGATTTCAAGTAATGCCACTCAAGTGTACAAGTCAAGCTAGAATGATGCTTTCTGTTTATGATTGTGATGGATATACATTGTCATGTGAAAAGGGTTGCCTTTTACTTGGTTGGAAAGGAAGGTCTATTATGATGGCTTCTGCTTGGCAAGTGGCAAGTTTGTCATATACTGATAGAGCCTCTTAA
Protein sequence

>MS.gene38845.t1

MEDLNMWLSFYMNESVNLDFPIRRFCPARMEQDQEGDCHDQETVLDDVELCTFDTTTTPCLLPSSEVDDFVDSFINMDQYEDQESQEKNHQSQNFDHHFLVNDDVFGYDPTTMEDEEFEICENTTITNEDEEVEMNGNFGAIPQSQASLGMNQGLDLVHTLLACAEAVGCRDNNQANLLLSKILALSSSSGDSLQRVSYCFATALKCRLSLYPHNVFSNSTLTTSTSNDVSLITRENKLEAFQLLYQTTPYITFGFMAANEAICQGSKGKSSIHIIDLGMEHTLQWPSLIRNLASRPEGPPKLRITGFSTNEENNAKLRASMNLLVEEALSLGIVLEFRIISEPATPSLLTIENLNLREGESLFVNSILKLHKYVKESRGYLKSILQSIKKLSPIAITVVEQDTNHNGPFFLGRFLESLHYYSAIFDSLEASMPRNSPIRMKIERVHFAEEICNIVACEGPDRMERHERVDQWRRQLGRAGFQVMPLKCTSQARMMLSVYDCDGYTLSCEKGCLLLGWKGRSIMMASAWQVASLSYTDRAS