Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38875.t1 | XP_003593687.1 | 88.3 | 77 | 9 | 0 | 7 | 83 | 54 | 130 | 1.10E-29 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38875.t1 | O82782 | 72.7 | 77 | 20 | 1 | 7 | 83 | 48 | 123 | 5.9e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38875.t1 | G7IFI7 | 88.3 | 77 | 9 | 0 | 7 | 83 | 54 | 130 | 7.9e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38875.t1 | MTR_2g015010 | 87.179 | 78 | 10 | 0 | 7 | 84 | 54 | 131 | 2.90e-41 | 140 |
| MS.gene38875.t1 | MTR_8g040300 | 90.625 | 32 | 3 | 0 | 101 | 132 | 113 | 144 | 1.64e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38875.t1 | AT2G36230 | 71.795 | 78 | 21 | 1 | 7 | 84 | 48 | 124 | 9.79e-31 | 113 |
Find 32 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCCTCTTTCAAATCTTTA+AGG | 0.074825 | 8.4:+51535771 | None:intergenic |
| TTCAAACAAGATGGACTTAA+AGG | 0.177160 | 8.4:-51535686 | MS.gene38875:CDS |
| TCAGGCTCAAAATTGGTAAT+TGG | 0.210433 | 8.4:+51535737 | None:intergenic |
| TAATGACATGCTATGAAATA+TGG | 0.297297 | 8.4:-51534790 | MS.gene38875:CDS |
| ATCCTCTTTCAAATCTTTAA+GGG | 0.339832 | 8.4:+51535772 | None:intergenic |
| AGATTTATCAGGCTCAAAAT+TGG | 0.342018 | 8.4:+51535730 | None:intergenic |
| TACTCAGCTGCAGATTTATC+AGG | 0.375812 | 8.4:+51535719 | None:intergenic |
| ACTTCAGCTCGCCGGTGACA+TGG | 0.438078 | 8.4:+51534323 | None:intergenic |
| GGGATAGTGAAACAAATTGT+TGG | 0.438108 | 8.4:-51535800 | MS.gene38875:CDS |
| AAAACAATTATTTCATCTAT+GGG | 0.471387 | 8.4:+51534378 | None:intergenic |
| GAAGCATTGCATGCTTATCC+TGG | 0.477302 | 8.4:-51535611 | MS.gene38875:intron |
| AAAGATTTGAAAGAGGATGA+TGG | 0.480092 | 8.4:-51535767 | MS.gene38875:CDS |
| GGATAGTGAAACAAATTGTT+GGG | 0.493596 | 8.4:-51535799 | MS.gene38875:CDS |
| GGTGGTCATGTTATCATGCT+TGG | 0.505881 | 8.4:-51535665 | MS.gene38875:CDS |
| ATTATTTCATCTATGGGCAA+TGG | 0.506531 | 8.4:+51534384 | None:intergenic |
| AACCCTTAAAGATTTGAAAG+AGG | 0.519640 | 8.4:-51535774 | MS.gene38875:CDS |
| TTACCTTGTGAATATGATGC+AGG | 0.525476 | 8.4:+51535935 | None:intergenic |
| AAGATTTGAAAGAGGATGAT+GGG | 0.525706 | 8.4:-51535766 | MS.gene38875:CDS |
| CTTCAGCTCGCCGGTGACAT+GGG | 0.527166 | 8.4:+51534324 | None:intergenic |
| AGAGAAGCAGCTTTACTCAA+AGG | 0.547387 | 8.4:+51535635 | None:intergenic |
| GAGCTTCCACTTCAGCTCGC+CGG | 0.548722 | 8.4:+51534315 | None:intergenic |
| AGAACTTTGTGATCTGTATG+AGG | 0.549999 | 8.4:+51534349 | None:intergenic |
| AAATTTGTTGCTTGATCACG+AGG | 0.558311 | 8.4:-51534530 | MS.gene38875:intron |
| AAAGTTCTCTCCCATGTCAC+CGG | 0.559302 | 8.4:-51534334 | MS.gene38875:CDS |
| GATGCAGGGGCGGAATTGAA+CGG | 0.566240 | 8.4:+51535950 | None:intergenic |
| TACCTTGTGAATATGATGCA+GGG | 0.569957 | 8.4:+51535936 | None:intergenic |
| AAACAAGATGGACTTAAAGG+TGG | 0.633622 | 8.4:-51535683 | MS.gene38875:CDS |
| TGATATATCTGTGAATAACA+TGG | 0.639776 | 8.4:+51534560 | None:intergenic |
| ACCTTGTGAATATGATGCAG+GGG | 0.643253 | 8.4:+51535937 | None:intergenic |
| GCCCCTGCATCATATTCACA+AGG | 0.667239 | 8.4:-51535938 | MS.gene38875:intron |
| TTGTGAATATGATGCAGGGG+CGG | 0.669612 | 8.4:+51535940 | None:intergenic |
| AATATGTCAATGTTTCCGTG+AGG | 0.732618 | 8.4:+51534593 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACAATTATTTCATCTA+TGG | + | chr8.4:51535907-51535926 | None:intergenic | 15.0% |
| !! | AAAACAATTATTTCATCTAT+GGG | + | chr8.4:51535906-51535925 | None:intergenic | 15.0% |
| !! | AAACTTACAAAAATCAATAT+AGG | + | chr8.4:51535365-51535384 | None:intergenic | 15.0% |
| !! | CAAATTACTAAAATAAAACA+CGG | + | chr8.4:51534878-51534897 | None:intergenic | 15.0% |
| !! | AAATTATAAGTAGCATAGAA+TGG | + | chr8.4:51535582-51535601 | None:intergenic | 20.0% |
| !! | ATACAAATAATTGCTACAAA+AGG | + | chr8.4:51535613-51535632 | None:intergenic | 20.0% |
| !! | ATACACATATTTCAAACTAA+AGG | - | chr8.4:51535200-51535219 | MS.gene38875:intron | 20.0% |
| !! | TGAACAGAAAAGTAAAAAAA+TGG | + | chr8.4:51534714-51534733 | None:intergenic | 20.0% |
| ! | ACAAAAATCAATATAGGACA+TGG | + | chr8.4:51535359-51535378 | None:intergenic | 25.0% |
| ! | ATCCTCTTTCAAATCTTTAA+GGG | + | chr8.4:51534512-51534531 | None:intergenic | 25.0% |
| ! | TAATGACATGCTATGAAATA+TGG | - | chr8.4:51535491-51535510 | MS.gene38875:intron | 25.0% |
| ! | TGATATATCTGTGAATAACA+TGG | + | chr8.4:51535724-51535743 | None:intergenic | 25.0% |
| ! | TGTAATCAAACTAAAATACC+AGG | + | chr8.4:51534691-51534710 | None:intergenic | 25.0% |
| !! | AAGGTATATCTAAGAAGTAT+TGG | + | chr8.4:51535125-51535144 | None:intergenic | 25.0% |
| !! | AATTTTACATGTGGGTTTTT+AGG | - | chr8.4:51534418-51534437 | MS.gene38875:intron | 25.0% |
| !! | AGAAGTTAATATTGACTGAA+AGG | + | chr8.4:51534373-51534392 | None:intergenic | 25.0% |
| !! | ATTTTACATGTGGGTTTTTA+GGG | - | chr8.4:51534419-51534438 | MS.gene38875:intron | 25.0% |
| !! | GAAGTTAATATTGACTGAAA+GGG | + | chr8.4:51534372-51534391 | None:intergenic | 25.0% |
| !!! | TTTCCACATTGATAATGTTT+TGG | - | chr8.4:51535006-51535025 | MS.gene38875:intron | 25.0% |
| AAAGATTTGAAAGAGGATGA+TGG | - | chr8.4:51534514-51534533 | MS.gene38875:intron | 30.0% | |
| AACATACATCGTGAAAAACA+TGG | + | chr8.4:51535551-51535570 | None:intergenic | 30.0% | |
| AACCCTTAAAGATTTGAAAG+AGG | - | chr8.4:51534507-51534526 | MS.gene38875:intron | 30.0% | |
| AAGATTTGAAAGAGGATGAT+GGG | - | chr8.4:51534515-51534534 | MS.gene38875:intron | 30.0% | |
| AGATTTATCAGGCTCAAAAT+TGG | + | chr8.4:51534554-51534573 | None:intergenic | 30.0% | |
| ATTATTTCATCTATGGGCAA+TGG | + | chr8.4:51535900-51535919 | None:intergenic | 30.0% | |
| CACAAAATATCACATGTGTT+TGG | + | chr8.4:51535237-51535256 | None:intergenic | 30.0% | |
| CATCCTCTTTCAAATCTTTA+AGG | + | chr8.4:51534513-51534532 | None:intergenic | 30.0% | |
| GATACTTTACGACACATATA+TGG | + | chr8.4:51535397-51535416 | None:intergenic | 30.0% | |
| GGATAGTGAAACAAATTGTT+GGG | - | chr8.4:51534482-51534501 | MS.gene38875:intron | 30.0% | |
| GTTCCAAAACATTATCAATG+TGG | + | chr8.4:51535012-51535031 | None:intergenic | 30.0% | |
| TAAGCCTTCAATTGATATTG+TGG | - | chr8.4:51534918-51534937 | MS.gene38875:intron | 30.0% | |
| TAGTCGAAGAGATGTTAAAA+CGG | + | chr8.4:51535843-51535862 | None:intergenic | 30.0% | |
| TCTGTATCATACTATCTATC+TGG | - | chr8.4:51535415-51535434 | MS.gene38875:intron | 30.0% | |
| TTAACTTCAACTCACTATTC+TGG | + | chr8.4:51535038-51535057 | None:intergenic | 30.0% | |
| ! | AAGGTACGAATTTTACATGT+GGG | - | chr8.4:51534410-51534429 | MS.gene38875:intron | 30.0% |
| ! | TGAGTTGAAGTTAACATTGA+AGG | - | chr8.4:51535044-51535063 | MS.gene38875:intron | 30.0% |
| ! | TGGTGTGTTTTGTAAAATTG+CGG | - | chr8.4:51534938-51534957 | MS.gene38875:intron | 30.0% |
| ! | TTAGTACATTTTAAGCCTCA+CGG | - | chr8.4:51535673-51535692 | MS.gene38875:CDS | 30.0% |
| ! | TTCAAACAAGATGGACTTAA+AGG | - | chr8.4:51534595-51534614 | MS.gene38875:CDS | 30.0% |
| !! | AAAGGTACGAATTTTACATG+TGG | - | chr8.4:51534409-51534428 | MS.gene38875:intron | 30.0% |
| !!! | GCAATGAAATTTTAAGACGT+GGG | - | chr8.4:51534441-51534460 | MS.gene38875:intron | 30.0% |
| AATATGTCAATGTTTCCGTG+AGG | + | chr8.4:51535691-51535710 | None:intergenic | 35.0% | |
| ACTATCTATCTGGACTTCTT+TGG | - | chr8.4:51535425-51535444 | MS.gene38875:intron | 35.0% | |
| AGAACTTTGTGATCTGTATG+AGG | + | chr8.4:51535935-51535954 | None:intergenic | 35.0% | |
| CACACCACAATATCAATTGA+AGG | + | chr8.4:51534925-51534944 | None:intergenic | 35.0% | |
| CAGTCAGAAATACATATCGA+AGG | + | chr8.4:51535144-51535163 | None:intergenic | 35.0% | |
| CTTGCCAATTCATGTCAAAA+AGG | - | chr8.4:51534391-51534410 | MS.gene38875:CDS | 35.0% | |
| GATGAGATCATCAGCATAAT+TGG | + | chr8.4:51535819-51535838 | None:intergenic | 35.0% | |
| GGGATAGTGAAACAAATTGT+TGG | - | chr8.4:51534481-51534500 | MS.gene38875:intron | 35.0% | |
| TACCTTGTGAATATGATGCA+GGG | + | chr8.4:51534348-51534367 | None:intergenic | 35.0% | |
| TGCATGGAAGGTTATCTTTA+AGG | - | chr8.4:51535074-51535093 | MS.gene38875:intron | 35.0% | |
| TTACCTTGTGAATATGATGC+AGG | + | chr8.4:51534349-51534368 | None:intergenic | 35.0% | |
| ! | AAACAAGATGGACTTAAAGG+TGG | - | chr8.4:51534598-51534617 | MS.gene38875:CDS | 35.0% |
| ! | CGTACCTTTTTGACATGAAT+TGG | + | chr8.4:51534398-51534417 | None:intergenic | 35.0% |
| ! | GCTACTCTTTTCAAACAAGA+TGG | - | chr8.4:51534586-51534605 | MS.gene38875:CDS | 35.0% |
| ! | TCAGGCTCAAAATTGGTAAT+TGG | + | chr8.4:51534547-51534566 | None:intergenic | 35.0% |
| ! | TGTGTTTTGTAAAATTGCGG+TGG | - | chr8.4:51534941-51534960 | MS.gene38875:intron | 35.0% |
| ! | TTTTCTGTTCATGCTTATCC+TGG | - | chr8.4:51534723-51534742 | MS.gene38875:intron | 35.0% |
| !! | AAATTTGTTGCTTGATCACG+AGG | - | chr8.4:51535751-51535770 | MS.gene38875:CDS | 35.0% |
| !! | TGGGTCATTGTGTTTTTGTA+GGG | - | chr8.4:51534460-51534479 | MS.gene38875:intron | 35.0% |
| !!! | GGCAATGAAATTTTAAGACG+TGG | - | chr8.4:51534440-51534459 | MS.gene38875:intron | 35.0% |
| ACAACTTACTTTGTTGCAGG+TGG | - | chr8.4:51534828-51534847 | MS.gene38875:intron | 40.0% | |
| ACCTTGTGAATATGATGCAG+GGG | + | chr8.4:51534347-51534366 | None:intergenic | 40.0% | |
| AGAGAAGCAGCTTTACTCAA+AGG | + | chr8.4:51534649-51534668 | None:intergenic | 40.0% | |
| CAACTTACTTTGTTGCAGGT+GGG | - | chr8.4:51534829-51534848 | MS.gene38875:intron | 40.0% | |
| GCGCAAGATGCAATACATTT+CGG | - | chr8.4:51534753-51534772 | MS.gene38875:intron | 40.0% | |
| TACTCAGCTGCAGATTTATC+AGG | + | chr8.4:51534565-51534584 | None:intergenic | 40.0% | |
| TGCACAACTTACTTTGTTGC+AGG | - | chr8.4:51534825-51534844 | MS.gene38875:intron | 40.0% | |
| ! | AAGTTGTGCAGAAAGTGTAG+TGG | + | chr8.4:51534815-51534834 | None:intergenic | 40.0% |
| !! | GGGTCATTGTGTTTTTGTAG+GGG | - | chr8.4:51534461-51534480 | MS.gene38875:intron | 40.0% |
| !! | GTGGGTCATTGTGTTTTTGT+AGG | - | chr8.4:51534459-51534478 | MS.gene38875:intron | 40.0% |
| AAAGTTCTCTCCCATGTCAC+CGG | - | chr8.4:51535947-51535966 | MS.gene38875:CDS | 45.0% | |
| AAGTAGCTTTCATGTCAGCG+TGG | + | chr8.4:51534971-51534990 | None:intergenic | 45.0% | |
| CATTGAAGGCTGTAGTTGCA+TGG | - | chr8.4:51535058-51535077 | MS.gene38875:intron | 45.0% | |
| CATTTCGGAGCTAGAACACA+AGG | - | chr8.4:51534768-51534787 | MS.gene38875:intron | 45.0% | |
| GGTGGTCATGTTATCATGCT+TGG | - | chr8.4:51534616-51534635 | MS.gene38875:intron | 45.0% | |
| TTGTGAATATGATGCAGGGG+CGG | + | chr8.4:51534344-51534363 | None:intergenic | 45.0% | |
| !! | GAAGCATTGCATGCTTATCC+TGG | - | chr8.4:51534670-51534689 | MS.gene38875:intron | 45.0% |
| GAAGGCTGTAGTTGCATGGA+AGG | - | chr8.4:51535062-51535081 | MS.gene38875:intron | 50.0% | |
| GCATCTTGCGCTCAAATACC+AGG | + | chr8.4:51534744-51534763 | None:intergenic | 50.0% | |
| GCCCCTGCATCATATTCACA+AGG | - | chr8.4:51534343-51534362 | MS.gene38875:CDS | 50.0% | |
| GATGCAGGGGCGGAATTGAA+CGG | + | chr8.4:51534334-51534353 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 51534326 | 51535977 | 51534326 | ID=MS.gene38875 |
| chr8.4 | mRNA | 51534326 | 51535977 | 51534326 | ID=MS.gene38875.t1;Parent=MS.gene38875 |
| chr8.4 | exon | 51535939 | 51535977 | 51535939 | ID=MS.gene38875.t1.exon1;Parent=MS.gene38875.t1 |
| chr8.4 | CDS | 51535939 | 51535977 | 51535939 | ID=cds.MS.gene38875.t1;Parent=MS.gene38875.t1 |
| chr8.4 | exon | 51535612 | 51535822 | 51535612 | ID=MS.gene38875.t1.exon2;Parent=MS.gene38875.t1 |
| chr8.4 | CDS | 51535612 | 51535822 | 51535612 | ID=cds.MS.gene38875.t1;Parent=MS.gene38875.t1 |
| chr8.4 | exon | 51534778 | 51534827 | 51534778 | ID=MS.gene38875.t1.exon3;Parent=MS.gene38875.t1 |
| chr8.4 | CDS | 51534778 | 51534827 | 51534778 | ID=cds.MS.gene38875.t1;Parent=MS.gene38875.t1 |
| chr8.4 | exon | 51534531 | 51534626 | 51534531 | ID=MS.gene38875.t1.exon4;Parent=MS.gene38875.t1 |
| chr8.4 | CDS | 51534531 | 51534626 | 51534531 | ID=cds.MS.gene38875.t1;Parent=MS.gene38875.t1 |
| chr8.4 | exon | 51534326 | 51534415 | 51534326 | ID=MS.gene38875.t1.exon5;Parent=MS.gene38875.t1 |
| chr8.4 | CDS | 51534326 | 51534415 | 51534326 | ID=cds.MS.gene38875.t1;Parent=MS.gene38875.t1 |
| Gene Sequence |
| Protein sequence |
