Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39102.t1 | XP_003625077.2 | 96.5 | 650 | 23 | 0 | 1 | 650 | 1 | 650 | 0.00E+00 | 1249.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39102.t1 | Q6ZW49 | 30.5 | 190 | 121 | 3 | 463 | 643 | 881 | 1068 | 1.4e-15 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39102.t1 | Q2HRG1 | 96.5 | 650 | 23 | 0 | 1 | 650 | 1 | 650 | 0.0e+00 | 1249.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene39102.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39102.t1 | MTR_7g090680 | 96.462 | 650 | 23 | 0 | 1 | 650 | 1 | 650 | 0.0 | 1310 |
| MS.gene39102.t1 | MTR_2g020440 | 25.000 | 196 | 140 | 2 | 449 | 639 | 930 | 1123 | 4.82e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39102.t1 | AT2G41450 | 47.139 | 664 | 282 | 11 | 1 | 647 | 1 | 612 | 0.0 | 573 |
| MS.gene39102.t1 | AT2G41450 | 49.073 | 593 | 244 | 8 | 68 | 647 | 2 | 549 | 0.0 | 545 |
| MS.gene39102.t1 | AT4G03130 | 27.317 | 205 | 131 | 7 | 449 | 644 | 566 | 761 | 3.15e-13 | 73.6 |
| MS.gene39102.t1 | AT4G03130 | 27.317 | 205 | 131 | 7 | 449 | 644 | 562 | 757 | 3.30e-13 | 73.6 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTCCCCATTCTTTGTTTA+AGG | 0.153944 | 7.4:-18123332 | MS.gene39102:CDS |
| ACAAAGAATCTGAAGGATTT+TGG | 0.192159 | 7.4:-18126909 | MS.gene39102:CDS |
| TTCTGTACTGCTCTCTGTTC+CGG | 0.211542 | 7.4:-18124955 | MS.gene39102:CDS |
| TCCAGTTTGGCTGGAGATTC+AGG | 0.213380 | 7.4:-18128126 | MS.gene39102:CDS |
| CTGATAAGGACTCTGCAAAT+TGG | 0.274898 | 7.4:+18122076 | None:intergenic |
| CAAACAATTCCTTCTTTAGA+TGG | 0.275506 | 7.4:+18126190 | None:intergenic |
| ATGAATTATGCTGCAAATAC+TGG | 0.277020 | 7.4:-18128339 | MS.gene39102:CDS |
| GTAATATCATCATCATCTTT+AGG | 0.278251 | 7.4:+18128528 | None:intergenic |
| TTTGACTTGTGTCTGTTCTA+TGG | 0.278979 | 7.4:+18126043 | None:intergenic |
| CGTAAAGCATTATGCTTTCC+TGG | 0.295086 | 7.4:-18126234 | MS.gene39102:CDS |
| GTGTCATTGCAGCAGTTCTT+AGG | 0.300365 | 7.4:+18125785 | None:intergenic |
| GTTGATATCCTGCAGGGTTT+TGG | 0.322827 | 7.4:-18127003 | MS.gene39102:intron |
| AATCTCACTCCTGAAGAAAA+AGG | 0.331775 | 7.4:-18128670 | MS.gene39102:intron |
| CTAAAGCATTGTTCAGTTGA+TGG | 0.343270 | 7.4:+18125428 | None:intergenic |
| TCTGTACTGCTCTCTGTTCC+GGG | 0.344750 | 7.4:-18124954 | MS.gene39102:intron |
| TGTCCCCATTCTTTGTTTAA+GGG | 0.350626 | 7.4:-18123331 | MS.gene39102:CDS |
| GATTAAGAGTTCGTTGGAAC+TGG | 0.354030 | 7.4:+18128846 | None:intergenic |
| TTCGTTGGAACTGGAATTGC+AGG | 0.354681 | 7.4:+18128855 | None:intergenic |
| TCCTGAATCTCCAGCCAAAC+TGG | 0.360192 | 7.4:+18128125 | None:intergenic |
| AGCGGAAATATCAAAGTATC+TGG | 0.362853 | 7.4:-18128811 | MS.gene39102:intron |
| CCCAATGCTTTGGTAAAAGT+TGG | 0.376995 | 7.4:-18126015 | MS.gene39102:CDS |
| CTTGAGAGATGCGTGACTAA+TGG | 0.377037 | 7.4:-18128300 | MS.gene39102:CDS |
| CACTACCTGAAGGTATGATT+TGG | 0.384533 | 7.4:+18128506 | None:intergenic |
| TTCTTGTATTAGGTTATTAG+TGG | 0.388331 | 7.4:-18122255 | MS.gene39102:intron |
| TTGAGAGATGCGTGACTAAT+GGG | 0.393773 | 7.4:-18128299 | MS.gene39102:intron |
| TTATTTGTTGAAGTATAAAT+CGG | 0.396914 | 7.4:-18123386 | MS.gene39102:CDS |
| TCTGCTATTGTTAGGTCTGC+TGG | 0.410098 | 7.4:-18123259 | MS.gene39102:CDS |
| TATAACCCTTAAACAAAGAA+TGG | 0.415431 | 7.4:+18123326 | None:intergenic |
| ACTACTTCTGAAATCCAGTT+TGG | 0.417176 | 7.4:-18128139 | MS.gene39102:CDS |
| AAACTCTGTCTGCTATTGTT+AGG | 0.420715 | 7.4:-18123267 | MS.gene39102:CDS |
| TGATAAGGACTCTGCAAATT+GGG | 0.421166 | 7.4:+18122077 | None:intergenic |
| TGGCTGCAAAGAAAGGTATA+AGG | 0.425240 | 7.4:-18122161 | MS.gene39102:CDS |
| ACTGTCGATCAGATTCTAGC+TGG | 0.443148 | 7.4:-18125586 | MS.gene39102:CDS |
| TTCAACAATTGCAGGTGTAA+AGG | 0.444936 | 7.4:+18126116 | None:intergenic |
| ACCTCACATTCTGAATGATA+AGG | 0.445474 | 7.4:-18123410 | MS.gene39102:CDS |
| TTGACTTGTGTCTGTTCTAT+GGG | 0.445981 | 7.4:+18126044 | None:intergenic |
| TTTCGACAAGGCAGATTTGT+TGG | 0.448692 | 7.4:-18123517 | MS.gene39102:intron |
| ATAACCCTTAAACAAAGAAT+GGG | 0.449312 | 7.4:+18123327 | None:intergenic |
| TCTGCATATTCTGTGTCTGC+AGG | 0.453750 | 7.4:+18127578 | None:intergenic |
| CGAGGATGAAGAGGCTTCCC+AGG | 0.455925 | 7.4:+18125646 | None:intergenic |
| TTTGCAGCATAATTCATTCC+AGG | 0.455995 | 7.4:+18128345 | None:intergenic |
| TCAAAGTAAACCCAATGCTT+TGG | 0.461826 | 7.4:-18126025 | MS.gene39102:CDS |
| CTTCTGAAATCCAGTTTGGC+TGG | 0.468826 | 7.4:-18128135 | MS.gene39102:CDS |
| TTGTTAACTACACAGTGCTT+GGG | 0.469784 | 7.4:-18123569 | MS.gene39102:intron |
| CTGATCGACAGTCAACAAAC+TGG | 0.471082 | 7.4:+18125597 | None:intergenic |
| TTTGATGACAATGCTAACTC+TGG | 0.474813 | 7.4:-18126171 | MS.gene39102:CDS |
| TCGGAGCTTCTAAATGCATA+TGG | 0.479623 | 7.4:+18125465 | None:intergenic |
| AAGAGAGATTAAGAGTTCGT+TGG | 0.488220 | 7.4:+18128840 | None:intergenic |
| AGATATTGTTTCTAGAGCTC+CGG | 0.491665 | 7.4:-18126978 | MS.gene39102:CDS |
| AGATTCACCGATGAAGATTG+AGG | 0.494644 | 7.4:+18129003 | None:intergenic |
| GGCATGTTAACAATCTGATA+AGG | 0.498089 | 7.4:+18122062 | None:intergenic |
| CATGATTCTCCATCTAAAGA+AGG | 0.505386 | 7.4:-18126199 | MS.gene39102:CDS |
| CTGTCGATCAGATTCTAGCT+GGG | 0.506118 | 7.4:-18125585 | MS.gene39102:CDS |
| AGACATTGAAGAAGCAATGA+TGG | 0.507527 | 7.4:-18122181 | MS.gene39102:CDS |
| GAATTCTGATGAGGCTGTGA+GGG | 0.507711 | 7.4:+18126138 | None:intergenic |
| CTTGGGTGGTCTCATCGACT+TGG | 0.511542 | 7.4:-18123552 | MS.gene39102:CDS |
| GCAATGATGGCTGCAAAGAA+AGG | 0.515441 | 7.4:-18122168 | MS.gene39102:CDS |
| TATTACCAAATCATACCTTC+AGG | 0.525549 | 7.4:-18128511 | MS.gene39102:intron |
| GGTGAAAGTGCTGGCTTCAA+CGG | 0.530210 | 7.4:+18128876 | None:intergenic |
| CGGAAGAGACTTCAAAATGT+TGG | 0.539655 | 7.4:-18126958 | MS.gene39102:CDS |
| GTTGGATCTTCACGAGGACG+TGG | 0.542047 | 7.4:-18125997 | MS.gene39102:CDS |
| GGATTCCGCAGAACAAGGAT+TGG | 0.544036 | 7.4:+18125538 | None:intergenic |
| ATAGCACAGGTGGATACAAA+AGG | 0.544150 | 7.4:-18126291 | MS.gene39102:CDS |
| TGACTTCAACGAGGATGAAG+AGG | 0.545463 | 7.4:+18125637 | None:intergenic |
| GGTGGAACTATTGCCTCTGA+TGG | 0.550900 | 7.4:-18125027 | MS.gene39102:CDS |
| CGAGGACGTGGAAAACTCAA+TGG | 0.557908 | 7.4:-18125985 | MS.gene39102:intron |
| TAATTGAAGTATCACAATGA+GGG | 0.558462 | 7.4:+18125516 | None:intergenic |
| AATGATGACAGACAAGCAAA+TGG | 0.562041 | 7.4:-18125743 | MS.gene39102:CDS |
| AAACATTCCTCAATCTTCAT+CGG | 0.570166 | 7.4:-18129010 | MS.gene39102:intron |
| GGAATTGCAGGTGAAAGTGC+TGG | 0.574962 | 7.4:+18128867 | None:intergenic |
| ATACAAAAGGTAGAGCCCGC+AGG | 0.578945 | 7.4:-18126278 | MS.gene39102:CDS |
| TGAGGGGATTCCGCAGAACA+AGG | 0.583621 | 7.4:+18125533 | None:intergenic |
| CTGCATATTCTGTGTCTGCA+GGG | 0.585523 | 7.4:+18127579 | None:intergenic |
| ATGTACATGACAGAGCTACC+TGG | 0.586083 | 7.4:-18128363 | MS.gene39102:CDS |
| TAAGGACTTTCGACAGTGAG+TGG | 0.588593 | 7.4:-18122143 | MS.gene39102:CDS |
| TTAACTACACAGTGCTTGGG+TGG | 0.593202 | 7.4:-18123566 | MS.gene39102:intron |
| TGTGGTTGAGCTTCCATCAG+AGG | 0.594722 | 7.4:+18125014 | None:intergenic |
| AGGATTGGCCCTAACAGCCT+CGG | 0.598648 | 7.4:+18125553 | None:intergenic |
| TCCTTATCATTCAGAATGTG+AGG | 0.599269 | 7.4:+18123409 | None:intergenic |
| CGTTGAAGTCAAAGAGAGTG+AGG | 0.602805 | 7.4:-18125625 | MS.gene39102:CDS |
| TCAACCACACATGTTGTCAC+AGG | 0.605648 | 7.4:-18125000 | MS.gene39102:CDS |
| TAAGAAAACACAGCTTACAA+AGG | 0.606148 | 7.4:-18125375 | MS.gene39102:intron |
| TGTCGATCAGATTCTAGCTG+GGG | 0.610722 | 7.4:-18125584 | MS.gene39102:CDS |
| TTGATGACAATGCTAACTCT+GGG | 0.616144 | 7.4:-18126170 | MS.gene39102:CDS |
| CATGAGACAAGAGCTCGACA+TGG | 0.620599 | 7.4:-18122106 | MS.gene39102:CDS |
| TTGAAGTCAAAGAGAGTGAG+GGG | 0.624778 | 7.4:-18125623 | MS.gene39102:CDS |
| GTAATTGAAGTATCACAATG+AGG | 0.625076 | 7.4:+18125515 | None:intergenic |
| TAGGGCCAATCCTTGTTCTG+CGG | 0.625365 | 7.4:-18125543 | MS.gene39102:CDS |
| AATTGAAGTATCACAATGAG+GGG | 0.625488 | 7.4:+18125517 | None:intergenic |
| TGGGGAGACAAAGAATCTGA+AGG | 0.626446 | 7.4:-18126916 | MS.gene39102:CDS |
| CAGAGGCAATAGTTCCACCT+AGG | 0.629274 | 7.4:+18125031 | None:intergenic |
| GTTGAAGTCAAAGAGAGTGA+GGG | 0.634453 | 7.4:-18125624 | MS.gene39102:CDS |
| CAATGCAATCTATCAACGGA+AGG | 0.636176 | 7.4:-18127537 | MS.gene39102:intron |
| CTGTCAATGCAATCTATCAA+CGG | 0.636846 | 7.4:-18127541 | MS.gene39102:CDS |
| ATTGCATTGACAGCTGCAAG+TGG | 0.637300 | 7.4:+18127551 | None:intergenic |
| ATCATGAGAGTCGAACCACC+AGG | 0.638186 | 7.4:+18126216 | None:intergenic |
| GGAATTCTGATGAGGCTGTG+AGG | 0.653417 | 7.4:+18126137 | None:intergenic |
| TTGCATTGACAGCTGCAAGT+GGG | 0.654261 | 7.4:+18127552 | None:intergenic |
| AATTCTGATGAGGCTGTGAG+GGG | 0.660055 | 7.4:+18126139 | None:intergenic |
| TTTGGCTGGAGATTCAGGAA+AGG | 0.669858 | 7.4:-18128121 | MS.gene39102:intron |
| ATATAGGTGATTGAAGACCT+AGG | 0.671284 | 7.4:-18125048 | MS.gene39102:intron |
| AAATGGTGCAGATTGTACCC+AGG | 0.675885 | 7.4:-18125726 | MS.gene39102:CDS |
| AAAGCATTATGCTTTCCTGG+TGG | 0.677104 | 7.4:-18126231 | MS.gene39102:CDS |
| GTAAAAGTTGGATCTTCACG+AGG | 0.678966 | 7.4:-18126003 | MS.gene39102:CDS |
| TTAATCTCTCTTCCTCGTAG+CGG | 0.679130 | 7.4:-18128829 | MS.gene39102:CDS |
| TTGTCCTGTGACAACATGTG+TGG | 0.679152 | 7.4:+18124996 | None:intergenic |
| TAAGGACTCTGCAAATTGGG+AGG | 0.680636 | 7.4:+18122080 | None:intergenic |
| GCTATTGTTAGGTCTGCTGG+TGG | 0.684582 | 7.4:-18123256 | MS.gene39102:CDS |
| TAACCCTTAAACAAAGAATG+GGG | 0.687115 | 7.4:+18123328 | None:intergenic |
| CACTCTCTTTGACTTCAACG+AGG | 0.710470 | 7.4:+18125628 | None:intergenic |
| GGTGTAAAGGAATTCTGATG+AGG | 0.724005 | 7.4:+18126129 | None:intergenic |
| ACTTTGATATTTCCGCTACG+AGG | 0.724866 | 7.4:+18128817 | None:intergenic |
| TAGGTGATTGAAGACCTAGG+TGG | 0.771557 | 7.4:-18125045 | MS.gene39102:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 18122068 | 18129047 | 18122068 | ID=MS.gene39102 |
| chr7.4 | mRNA | 18122068 | 18129047 | 18122068 | ID=MS.gene39102.t1;Parent=MS.gene39102 |
| chr7.4 | exon | 18129011 | 18129047 | 18129011 | ID=MS.gene39102.t1.exon1;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18129011 | 18129047 | 18129011 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18128812 | 18128913 | 18128812 | ID=MS.gene39102.t1.exon2;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18128812 | 18128913 | 18128812 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18128671 | 18128724 | 18128671 | ID=MS.gene39102.t1.exon3;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18128671 | 18128724 | 18128671 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18128512 | 18128573 | 18128512 | ID=MS.gene39102.t1.exon4;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18128512 | 18128573 | 18128512 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18128300 | 18128397 | 18128300 | ID=MS.gene39102.t1.exon5;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18128300 | 18128397 | 18128300 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18128122 | 18128173 | 18128122 | ID=MS.gene39102.t1.exon6;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18128122 | 18128173 | 18128122 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18127538 | 18127630 | 18127538 | ID=MS.gene39102.t1.exon7;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18127538 | 18127630 | 18127538 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18126900 | 18127010 | 18126900 | ID=MS.gene39102.t1.exon8;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18126900 | 18127010 | 18126900 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18125986 | 18126325 | 18125986 | ID=MS.gene39102.t1.exon9;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18125986 | 18126325 | 18125986 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18125376 | 18125824 | 18125376 | ID=MS.gene39102.t1.exon10;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18125376 | 18125824 | 18125376 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18124955 | 18125064 | 18124955 | ID=MS.gene39102.t1.exon11;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18124955 | 18125064 | 18124955 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18123518 | 18123576 | 18123518 | ID=MS.gene39102.t1.exon12;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18123518 | 18123576 | 18123518 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18123252 | 18123439 | 18123252 | ID=MS.gene39102.t1.exon13;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18123252 | 18123439 | 18123252 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| chr7.4 | exon | 18122068 | 18122265 | 18122068 | ID=MS.gene39102.t1.exon14;Parent=MS.gene39102.t1 |
| chr7.4 | CDS | 18122068 | 18122265 | 18122068 | ID=cds.MS.gene39102.t1;Parent=MS.gene39102.t1 |
| Gene Sequence |
| Protein sequence |
