AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39449

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39449.t1 MTR_4g088900 97.143 210 6 0 1 210 1 210 3.47e-153 423
MS.gene39449.t1 MTR_7g091370 45.143 175 76 4 31 198 20 181 2.24e-40 137
MS.gene39449.t1 MTR_5g010680 38.614 202 87 4 30 198 21 218 2.33e-40 141
MS.gene39449.t1 MTR_1g100777 37.255 204 102 4 17 202 4 199 1.77e-37 132
MS.gene39449.t1 MTR_1g071360 42.135 178 84 4 31 201 30 195 2.46e-36 130
MS.gene39449.t1 MTR_1g071360 42.135 178 84 4 31 201 27 192 3.34e-36 130
MS.gene39449.t1 MTR_5g089170 39.326 178 96 3 30 199 22 195 5.04e-36 127
MS.gene39449.t1 MTR_3g101870 35.981 214 89 3 30 199 21 230 4.68e-35 127
MS.gene39449.t1 MTR_0020s0020 56.122 98 39 1 31 128 31 124 7.54e-35 126
MS.gene39449.t1 MTR_6g043060 56.701 97 38 1 32 128 21 113 1.68e-34 125
MS.gene39449.t1 MTR_1g102860 43.558 163 83 3 31 191 38 193 4.29e-34 124
MS.gene39449.t1 MTR_5g029680 55.556 99 40 1 30 128 29 123 1.41e-33 125
MS.gene39449.t1 MTR_5g017470 52.525 99 43 1 30 128 6 100 1.67e-33 121
MS.gene39449.t1 MTR_2g100670 40.678 177 90 5 31 206 31 193 1.10e-32 120
MS.gene39449.t1 MTR_1g061170 39.665 179 97 5 2 174 14 187 1.33e-32 122
MS.gene39449.t1 MTR_7g095680 54.082 98 41 1 31 128 47 140 4.04e-32 119
MS.gene39449.t1 MTR_2g100670 40.678 177 90 5 31 206 139 301 5.57e-32 120
MS.gene39449.t1 MTR_2g100680 53.061 98 42 1 31 128 29 122 9.89e-32 120
MS.gene39449.t1 MTR_4g077970 55.670 97 39 1 33 129 12 104 2.53e-31 119
MS.gene39449.t1 MTR_8g083100 55.789 95 38 1 34 128 23 113 5.33e-31 118
MS.gene39449.t1 MTR_3g104550 39.181 171 94 4 31 198 44 207 2.71e-30 115
MS.gene39449.t1 MTR_8g087540 39.597 149 75 3 33 181 12 145 3.84e-30 115
MS.gene39449.t1 MTR_2g017890 36.527 167 93 3 33 199 15 168 9.92e-28 108
MS.gene39449.t1 MTR_6g086805 49.485 97 45 1 32 128 10 102 3.40e-27 106
MS.gene39449.t1 MTR_8g105780 40.645 155 82 5 32 185 21 166 9.89e-27 105
MS.gene39449.t1 MTR_4g045757 51.429 70 29 2 33 101 14 79 3.73e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39449.t1 AT5G62020 35.204 196 97 3 30 199 20 211 2.74e-37 131
MS.gene39449.t1 AT4G36990 37.441 211 100 5 22 210 3 203 1.28e-36 129
MS.gene39449.t1 AT2G41690 38.660 194 99 4 21 198 28 217 1.46e-35 125
MS.gene39449.t1 AT4G17750 57.000 100 39 1 30 129 49 144 7.35e-35 129
MS.gene39449.t1 AT1G46264 55.000 100 41 1 30 129 31 126 1.98e-34 125
MS.gene39449.t1 AT4G11660 55.000 100 41 1 30 129 56 151 1.34e-33 124
MS.gene39449.t1 AT4G13980 38.333 180 89 3 34 210 24 184 1.95e-33 124
MS.gene39449.t1 AT5G03720 40.476 168 81 4 22 180 35 192 5.59e-33 122
MS.gene39449.t1 AT5G03720 40.476 168 81 4 22 180 35 192 5.59e-33 122
MS.gene39449.t1 AT5G43840 51.818 110 49 1 31 140 17 122 5.93e-33 120
MS.gene39449.t1 AT3G22830 53.535 99 42 1 31 129 59 153 1.16e-32 122
MS.gene39449.t1 AT1G32330 53.535 99 42 1 30 128 34 128 1.23e-32 122
MS.gene39449.t1 AT1G32330 53.535 99 42 1 30 128 34 128 1.52e-32 122
MS.gene39449.t1 AT5G16820 53.922 102 43 1 27 128 21 118 6.19e-32 120
MS.gene39449.t1 AT5G16820 53.922 102 43 1 27 128 21 118 6.19e-32 120
MS.gene39449.t1 AT2G26150 39.881 168 85 4 31 192 42 199 1.55e-31 117
MS.gene39449.t1 AT2G26150 39.881 168 85 4 31 192 42 199 1.55e-31 117
MS.gene39449.t1 AT3G63350 54.639 97 40 1 31 127 26 118 1.45e-30 114
MS.gene39449.t1 AT3G51910 48.696 115 53 3 30 143 26 135 1.65e-30 113
MS.gene39449.t1 AT3G02990 53.125 96 41 1 33 128 23 114 5.89e-29 112
MS.gene39449.t1 AT3G24520 46.729 107 53 1 32 138 16 118 1.14e-28 110
MS.gene39449.t1 AT5G54070 52.083 96 42 1 32 127 70 161 1.53e-28 109
MS.gene39449.t1 AT4G18880 38.830 188 94 6 21 203 6 177 4.37e-26 103
MS.gene39449.t1 AT5G45710 38.983 177 93 5 33 206 13 177 3.29e-25 100
MS.gene39449.t1 AT5G45710 38.983 177 93 5 33 206 13 177 3.29e-25 100
MS.gene39449.t1 AT1G67970 46.939 98 47 2 32 128 18 111 3.51e-24 98.6
MS.gene39449.t1 AT4G18870 39.130 92 51 2 33 124 146 232 6.57e-15 72.0
MS.gene39449.t1 AT4G18870 35.185 108 61 4 28 133 8 108 1.56e-12 65.5

Find 41 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGTCTCCTAACTTTATTT+TGG 0.243004 4.2:+22136607 None:intergenic
TTGTTGTGCTTGAAATATTT+AGG 0.265731 4.2:+22138013 None:intergenic
ACTCTAACCTCTCCTTCTTT+AGG 0.266225 4.2:+22136706 None:intergenic
AATGGTGCAGGGCACCTTAA+TGG 0.303317 4.2:+22138166 None:intergenic
TCTATTGAAGACCTATGATT+TGG 0.323717 4.2:-22138143 MS.gene39449:CDS
AGAAATTTCAAAAGGGTTGC+AGG 0.342009 4.2:-22136881 MS.gene39449:CDS
GAGTGTCCCTTAAGCCATTA+AGG 0.355422 4.2:-22138180 MS.gene39449:CDS
CCAGCATGAGAAATTTCAAA+AGG 0.385876 4.2:-22136889 MS.gene39449:CDS
CAGGGCACCTTAATGGCTTA+AGG 0.389744 4.2:+22138173 None:intergenic
AGAAAATATCATCTAAAAGA+TGG 0.402907 4.2:-22136917 MS.gene39449:CDS
TCATAGGTCTTCAATAGAAA+TGG 0.435601 4.2:+22138148 None:intergenic
ATAACCGCCAAAATAAAGTT+AGG 0.439240 4.2:-22136614 MS.gene39449:CDS
GGCACTTGAAATGAAGTTGT+TGG 0.448229 4.2:-22136658 MS.gene39449:CDS
GGAGTGTCTCTCTCAGCATA+TGG 0.469453 4.2:-22136637 MS.gene39449:CDS
GAACAAGACTTGAGATATTG+AGG 0.477443 4.2:+22136810 None:intergenic
CAGCATGAGAAATTTCAAAA+GGG 0.481854 4.2:-22136888 MS.gene39449:CDS
ATTGAAGACCTATGATTTGG+TGG 0.487727 4.2:-22138140 MS.gene39449:CDS
CAAGGTAGTGAAGGTGAAGA+TGG 0.491452 4.2:-22138115 MS.gene39449:CDS
GTCTTCAATAGAAATGGTGC+AGG 0.494487 4.2:+22138154 None:intergenic
ACCTTGTTCCACCAAATCAT+AGG 0.508730 4.2:+22138132 None:intergenic
GAAAATATCATCTAAAAGAT+GGG 0.510989 4.2:-22136916 MS.gene39449:CDS
TCATGGAATGAAGAAGGAAA+TGG 0.522950 4.2:-22138079 MS.gene39449:CDS
AGGGCACCTTAATGGCTTAA+GGG 0.526456 4.2:+22138174 None:intergenic
AGATATTGAGGAAACACGCT+TGG 0.529081 4.2:+22136822 None:intergenic
TAGCAATCATGAACTACTCA+TGG 0.534294 4.2:-22136742 MS.gene39449:CDS
GTGAGCTCTGAGAATTCAGC+TGG 0.540598 4.2:+22138040 None:intergenic
AAAGGGTTGCAGGCATATGT+TGG 0.543171 4.2:-22136871 MS.gene39449:CDS
ATTGTGTCATGGAATGAAGA+AGG 0.551158 4.2:-22138085 MS.gene39449:CDS
GGAGAACAAGAACCTAAAGA+AGG 0.564741 4.2:-22136718 MS.gene39449:CDS
ACCTATGATTTGGTGGAACA+AGG 0.572095 4.2:-22138133 MS.gene39449:CDS
AATGTGATGGAGCTAAACTT+TGG 0.582577 4.2:-22138235 MS.gene39449:CDS
ACAAGAACCTAAAGAAGGAG+AGG 0.585621 4.2:-22136713 MS.gene39449:CDS
ATGGCATTAAAATTGTGTCA+TGG 0.592944 4.2:-22138096 MS.gene39449:CDS
AATGCAAATAGCTGAATGCA+AGG 0.596737 4.2:-22136679 MS.gene39449:CDS
TAACATGAGTGGAAATGTGA+TGG 0.602953 4.2:-22138248 None:intergenic
GTGCTTGAAATATTTAGGCA+AGG 0.616604 4.2:+22138018 None:intergenic
ATATGTTGGTTGAAATCAGT+AGG 0.621529 4.2:-22136857 MS.gene39449:CDS
ATATCTCAAGTCTTGTTCAG+AGG 0.622749 4.2:-22136805 MS.gene39449:CDS
TTGGTGGAACAAGGTAGTGA+AGG 0.622819 4.2:-22138124 MS.gene39449:CDS
TCTTCAATAGAAATGGTGCA+GGG 0.670077 4.2:+22138155 None:intergenic
CAATCATGAACTACTCATGG+AGG 0.704767 4.2:-22136739 MS.gene39449:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTCTTTGAATTACTT+GGG - chr4.2:22137267-22137286 MS.gene39449:intron 15.0%
!!! ATGACTAATATTTCTTTTTT+AGG + chr4.2:22137091-22137110 None:intergenic 15.0%
!! AAAATTCTTTGAATTACTTG+GGG - chr4.2:22137268-22137287 MS.gene39449:intron 20.0%
!! ACTAATTAATTACTTCAACA+GGG - chr4.2:22137901-22137920 MS.gene39449:intron 20.0%
!! AGAAAATATCATCTAAAAGA+TGG - chr4.2:22137929-22137948 MS.gene39449:intron 20.0%
!! ATTGTTCTATACTTTCTATT+AGG + chr4.2:22137011-22137030 None:intergenic 20.0%
!! CAAAAATTCTTTGAATTACT+TGG - chr4.2:22137266-22137285 MS.gene39449:intron 20.0%
!! GAAAATATCATCTAAAAGAT+GGG - chr4.2:22137930-22137949 MS.gene39449:intron 20.0%
!!! AAATCTCACTTTATTTTGAA+TGG - chr4.2:22136971-22136990 MS.gene39449:intron 20.0%
!!! AGTAATTCAAAGAATTTTTG+AGG + chr4.2:22137266-22137285 None:intergenic 20.0%
!!! ATTGATATATTGATTCATCA+AGG - chr4.2:22137167-22137186 MS.gene39449:intron 20.0%
!!! TAATAGAAAGTATAGAACAA+TGG - chr4.2:22137010-22137029 MS.gene39449:intron 20.0%
!!! TAATATCAACATTCTTTTGA+AGG + chr4.2:22137128-22137147 None:intergenic 20.0%
!!! TTGATATATTGATTCATCAA+GGG - chr4.2:22137168-22137187 MS.gene39449:intron 20.0%
! AAATTCTTTGAATTACTTGG+GGG - chr4.2:22137269-22137288 MS.gene39449:intron 25.0%
! AGTTAGTGACACAAATTATA+AGG - chr4.2:22137589-22137608 MS.gene39449:intron 25.0%
! ATAGTTAACCTTCATATTAG+TGG + chr4.2:22137048-22137067 None:intergenic 25.0%
! ATGCATATATTAACACAACA+TGG - chr4.2:22137828-22137847 MS.gene39449:intron 25.0%
! CACTAATTAATTACTTCAAC+AGG - chr4.2:22137900-22137919 MS.gene39449:intron 25.0%
! CAGTGAACAAACAAAAAATT+CGG + chr4.2:22137222-22137241 None:intergenic 25.0%
! CTAATTAATTACTTCAACAG+GGG - chr4.2:22137902-22137921 MS.gene39449:intron 25.0%
! TGCTATATTTAACGTTATAC+TGG - chr4.2:22137659-22137678 MS.gene39449:intron 25.0%
! TTGTTGTGCTTGAAATATTT+AGG + chr4.2:22136836-22136855 None:intergenic 25.0%
!! AAAAGACCGAATTTGAAATT+TGG - chr4.2:22137410-22137429 MS.gene39449:intron 25.0%
!! CAATTTTCTTCTGATCAAAA+TGG - chr4.2:22136644-22136663 MS.gene39449:CDS 25.0%
!!! ATAGTCTCCTAACTTTATTT+TGG + chr4.2:22138242-22138261 None:intergenic 25.0%
!!! ATTCTTTTGAAGGAATAAAC+AGG + chr4.2:22137118-22137137 None:intergenic 25.0%
!!! CATTTTGATCAGAAGAAAAT+TGG + chr4.2:22136646-22136665 None:intergenic 25.0%
ATAACCGCCAAAATAAAGTT+AGG - chr4.2:22138232-22138251 MS.gene39449:CDS 30.0%
ATGGCATTAAAATTGTGTCA+TGG - chr4.2:22136750-22136769 MS.gene39449:CDS 30.0%
CAGCATGAGAAATTTCAAAA+GGG - chr4.2:22137958-22137977 MS.gene39449:intron 30.0%
GATGGTCCAAATTTCAAATT+CGG + chr4.2:22137419-22137438 None:intergenic 30.0%
GGAGTAAAAACTCAAAAACT+TGG + chr4.2:22137201-22137220 None:intergenic 30.0%
TCATAGGTCTTCAATAGAAA+TGG + chr4.2:22136701-22136720 None:intergenic 30.0%
TCTATTGAAGACCTATGATT+TGG - chr4.2:22136703-22136722 MS.gene39449:CDS 30.0%
TTAACGTTATACTGGTACTT+GGG - chr4.2:22137667-22137686 MS.gene39449:intron 30.0%
TTTAACGTTATACTGGTACT+TGG - chr4.2:22137666-22137685 MS.gene39449:intron 30.0%
TTTAGAGAGATAATCGATCA+AGG + chr4.2:22136903-22136922 None:intergenic 30.0%
TTTGTTGAATCGAAATTGGA+TGG + chr4.2:22137437-22137456 None:intergenic 30.0%
! ATATGTTGGTTGAAATCAGT+AGG - chr4.2:22137989-22138008 MS.gene39449:CDS 30.0%
! GTCATTTGTTGAATCGAAAT+TGG + chr4.2:22137441-22137460 None:intergenic 30.0%
!! AAAGAATTTTTGAGGAAGCT+AGG + chr4.2:22137258-22137277 None:intergenic 30.0%
!! AAGAATTTTTGAGGAAGCTA+GGG + chr4.2:22137257-22137276 None:intergenic 30.0%
!!! CGAATTTTTTGTTTGTTCAC+TGG - chr4.2:22137220-22137239 MS.gene39449:intron 30.0%
AAAGAGCTACTGCAAAGTAA+TGG + chr4.2:22137501-22137520 None:intergenic 35.0%
AACAAATGACTCTAACTGTG+TGG - chr4.2:22137451-22137470 MS.gene39449:intron 35.0%
AATGCAAATAGCTGAATGCA+AGG - chr4.2:22138167-22138186 MS.gene39449:CDS 35.0%
AATTCATGCAGTCAAGACAT+CGG - chr4.2:22137321-22137340 MS.gene39449:intron 35.0%
AGAAATTTCAAAAGGGTTGC+AGG - chr4.2:22137965-22137984 MS.gene39449:intron 35.0%
ATTACTTTGCAGTAGCTCTT+TGG - chr4.2:22137500-22137519 MS.gene39449:intron 35.0%
ATTGTGTCATGGAATGAAGA+AGG - chr4.2:22136761-22136780 MS.gene39449:CDS 35.0%
CCAGCATGAGAAATTTCAAA+AGG - chr4.2:22137957-22137976 MS.gene39449:intron 35.0%
CGTGCACAAAATGAAGAATT+TGG - chr4.2:22137563-22137582 MS.gene39449:intron 35.0%
GAACAAGACTTGAGATATTG+AGG + chr4.2:22138039-22138058 None:intergenic 35.0%
GTGCTTGAAATATTTAGGCA+AGG + chr4.2:22136831-22136850 None:intergenic 35.0%
TAGCAATCATGAACTACTCA+TGG - chr4.2:22138104-22138123 MS.gene39449:CDS 35.0%
TCATGGAATGAAGAAGGAAA+TGG - chr4.2:22136767-22136786 MS.gene39449:CDS 35.0%
TCTTCAATAGAAATGGTGCA+GGG + chr4.2:22136694-22136713 None:intergenic 35.0%
! AATGTGATGGAGCTAAACTT+TGG - chr4.2:22136611-22136630 MS.gene39449:CDS 35.0%
! AATTCTTCATTTTGTGCACG+TGG + chr4.2:22137563-22137582 None:intergenic 35.0%
! ATTCTTCATTTTGTGCACGT+GGG + chr4.2:22137562-22137581 None:intergenic 35.0%
! ATTGAAGACCTATGATTTGG+TGG - chr4.2:22136706-22136725 MS.gene39449:CDS 35.0%
!! ATATCTCAAGTCTTGTTCAG+AGG - chr4.2:22138041-22138060 MS.gene39449:CDS 35.0%
!! CTACTGCAAAGTAATGGATT+TGG + chr4.2:22137495-22137514 None:intergenic 35.0%
!! TACACGACGATATTGACTTT+GGG + chr4.2:22137635-22137654 None:intergenic 35.0%
!!! AAGGAAATGGTTTTGTTGTG+TGG - chr4.2:22136780-22136799 MS.gene39449:CDS 35.0%
!!! CCTTTTGAAATTTCTCATGC+TGG + chr4.2:22137960-22137979 None:intergenic 35.0%
!!! GTCTCCTAACTTTATTTTGG+CGG + chr4.2:22138239-22138258 None:intergenic 35.0%
ACAAGAACCTAAAGAAGGAG+AGG - chr4.2:22138133-22138152 MS.gene39449:CDS 40.0%
ACCTTGTTCCACCAAATCAT+AGG + chr4.2:22136717-22136736 None:intergenic 40.0%
ACTCTAACCTCTCCTTCTTT+AGG + chr4.2:22138143-22138162 None:intergenic 40.0%
AGATATTGAGGAAACACGCT+TGG + chr4.2:22138027-22138046 None:intergenic 40.0%
CAATCATGAACTACTCATGG+AGG - chr4.2:22138107-22138126 MS.gene39449:CDS 40.0%
CCTTCATATTAGTGGCAGAA+AGG + chr4.2:22137040-22137059 None:intergenic 40.0%
CCTTTCTGCCACTAATATGA+AGG - chr4.2:22137037-22137056 MS.gene39449:intron 40.0%
CTTTGAATTACTTGGGGGAT+TGG - chr4.2:22137274-22137293 MS.gene39449:intron 40.0%
GAGAGATAATCGATCAAGGT+AGG + chr4.2:22136899-22136918 None:intergenic 40.0%
GGAGAACAAGAACCTAAAGA+AGG - chr4.2:22138128-22138147 MS.gene39449:CDS 40.0%
GGCACTTGAAATGAAGTTGT+TGG - chr4.2:22138188-22138207 MS.gene39449:CDS 40.0%
GTCTTCAATAGAAATGGTGC+AGG + chr4.2:22136695-22136714 None:intergenic 40.0%
!! ACCTATGATTTGGTGGAACA+AGG - chr4.2:22136713-22136732 MS.gene39449:CDS 40.0%
!! AGTAATGGATTTGGATCTGC+TGG + chr4.2:22137486-22137505 None:intergenic 40.0%
!! GTAATGGATTTGGATCTGCT+GGG + chr4.2:22137485-22137504 None:intergenic 40.0%
!! GTACACGACGATATTGACTT+TGG + chr4.2:22137636-22137655 None:intergenic 40.0%
AAAGGGTTGCAGGCATATGT+TGG - chr4.2:22137975-22137994 MS.gene39449:intron 45.0%
CAAGGTAGTGAAGGTGAAGA+TGG - chr4.2:22136731-22136750 MS.gene39449:CDS 45.0%
GAGTGTCCCTTAAGCCATTA+AGG - chr4.2:22136666-22136685 MS.gene39449:CDS 45.0%
GTCTTGACTGCATGAATTCG+TGG + chr4.2:22137319-22137338 None:intergenic 45.0%
TTGGTGGAACAAGGTAGTGA+AGG - chr4.2:22136722-22136741 MS.gene39449:CDS 45.0%
! AGGGCACCTTAATGGCTTAA+GGG + chr4.2:22136675-22136694 None:intergenic 45.0%
! AATGGTGCAGGGCACCTTAA+TGG + chr4.2:22136683-22136702 None:intergenic 50.0%
! CAGGGCACCTTAATGGCTTA+AGG + chr4.2:22136676-22136695 None:intergenic 50.0%
! CTTCATTTTGTGCACGTGGG+TGG + chr4.2:22137559-22137578 None:intergenic 50.0%
! GGAGTGTCTCTCTCAGCATA+TGG - chr4.2:22138209-22138228 MS.gene39449:CDS 50.0%
!! GTGAGCTCTGAGAATTCAGC+TGG + chr4.2:22136809-22136828 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22136602 22138266 22136602 ID=MS.gene39449
chr4.2 mRNA 22136602 22138266 22136602 ID=MS.gene39449.t1;Parent=MS.gene39449
chr4.2 exon 22137979 22138266 22137979 ID=MS.gene39449.t1.exon1;Parent=MS.gene39449.t1
chr4.2 CDS 22137979 22138266 22137979 ID=cds.MS.gene39449.t1;Parent=MS.gene39449.t1
chr4.2 exon 22136602 22136946 22136602 ID=MS.gene39449.t1.exon2;Parent=MS.gene39449.t1
chr4.2 CDS 22136602 22136946 22136602 ID=cds.MS.gene39449.t1;Parent=MS.gene39449.t1
Gene Sequence

>MS.gene39449

ATGAGTGGAAATGTGATGGAGCTAAACTTTGGTTCAATAGACCAATTTTCTTCTGATCAAAATGGAGTGTCCCTTAAGCCATTAAGGTGCCCTGCACCATTTCTATTGAAGACCTATGATTTGGTGGAACAAGGTAGTGAAGGTGAAGATGGCATTAAAATTGTGTCATGGAATGAAGAAGGAAATGGTTTTGTTGTGTGGTCACCAGCTGAATTCTCAGAGCTCACCTTGCCTAAATATTTCAAGCACAACAATTTCTCTAGCTTTATTCGACAACTCAATACATATGTAAGTCCTACCTTGATCGATTATCTCTCTAAACATCAAAAGAAAAATGTGATCTATATATTGATAAAAAGTTTAAAACACAAATCTCACTTTATTTTGAATGGTTTAATGAATTAGTCCTAATAGAAAGTATAGAACAATGGATTTCCTTTCTGCCACTAATATGAAGGTTAACTATTTACAAATTTATATTGCATTCCTAAAAAAGAAATATTAGTCATTGTTCCTGTTTATTCCTTCAAAAGAATGTTGATATTATTATGTCATACAAATATATATTGATATATTGATTCATCAAGGGTATCTTGCCAAGTTTTTGAGTTTTTACTCCGAATTTTTTGTTTGTTCACTGGTTTCTGTTCTTCCCTAGCTTCCTCAAAAATTCTTTGAATTACTTGGGGGATTGGATTTTCTCTAGATGACATTCCACGAATTCATGCAGTCAAGACATCGGTTGTTGTTTGATTGAAATCAATTTATTCATATTTTAGTGTATATTTTAATTTTTATTTTAAAAATTAAAAGACCGAATTTGAAATTTGGACCATCCAATTTCGATTCAACAAATGACTCTAACTGTGTGGATTGCTGACCCAGCAGATCCAAATCCATTACTTTGCAGTAGCTCTTTGGAAAAAGAAGTAGATGACAATTAAATTAAGAAATCCACCCACGTGCACAAAATGAAGAATTTGGTCAAGTTAGTGACACAAATTATAAGGTCAAATATCAGTTATTTTAACCCAAAGTCAATATCGTCGTGTACACATGCTATATTTAACGTTATACTGGTACTTGGGAGCACACTTAAATTGAAAATAATAGTAAAGATAATCTTTGCTTGTTATTCAGTTACATATTCAACAATATTGTCTTTGACTGTTATTTTGTTCATTTTTATTAAAAAAATATGAAGTACAATAGCACATAAATCATAAATGCATATATTAACACAACATGGAATGATATTGTAATGTTTCAAAGTATGATATTTGTTTTATGTAATCTCTCACTAATTAATTACTTCAACAGGGGTTTAAGAAAATATCATCTAAAAGATGGGAATTCCAGCATGAGAAATTTCAAAAGGGTTGCAGGCATATGTTGGTTGAAATCAGTAGGAAAAAGTGTGAGCCAAGCGTGTTTCCTCAATATCTCAAGTCTTGTTCAGAGGAAAATGCAGTGACTAATAATTCATCACTTGAAGAAGACAATAGCAATCATGAACTACTCATGGAGGAGAACAAGAACCTAAAGAAGGAGAGGTTAGAGTTGCAAATGCAAATAGCTGAATGCAAGGCACTTGAAATGAAGTTGTTGGAGTGTCTCTCTCAGCATATGGATAACCGCCAAAATAAAGTTAGGAGACTATGTTAA
Protein sequence

>MS.gene39449.t1

MSGNVMELNFGSIDQFSSDQNGVSLKPLRCPAPFLLKTYDLVEQGSEGEDGIKIVSWNEEGNGFVVWSPAEFSELTLPKYFKHNNFSSFIRQLNTYGFKKISSKRWEFQHEKFQKGCRHMLVEISRKKCEPSVFPQYLKSCSEENAVTNNSSLEEDNSNHELLMEENKNLKKERLELQMQIAECKALEMKLLECLSQHMDNRQNKVRRLC