AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39454

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39454.t1 MTR_4g088850 97.802 91 2 0 1 91 43 133 4.15e-62 184
MS.gene39454.t1 MTR_4g088850 97.143 70 2 0 1 70 43 112 4.34e-45 142
MS.gene39454.t1 MTR_4g098500 56.338 71 31 0 1 71 47 117 9.35e-25 90.1
MS.gene39454.t1 MTR_4g098500 56.338 71 31 0 1 71 73 143 2.78e-24 89.7
MS.gene39454.t1 MTR_2g047838 43.529 85 48 0 1 85 47 131 3.08e-24 90.1
MS.gene39454.t1 MTR_8g089310 51.316 76 37 0 1 76 44 119 4.93e-23 87.0
MS.gene39454.t1 MTR_8g089310 51.316 76 37 0 1 76 57 132 6.49e-23 87.0
MS.gene39454.t1 MTR_8g089310 51.316 76 37 0 1 76 91 166 1.42e-22 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39454.t1 AT4G21610 75.309 81 20 0 1 81 61 141 7.97e-41 132
MS.gene39454.t1 AT4G21610 75.362 69 17 0 1 69 61 129 3.54e-32 109
MS.gene39454.t1 AT1G32540 54.930 71 32 0 1 71 74 144 4.70e-24 89.4
MS.gene39454.t1 AT1G32540 54.930 71 32 0 1 71 74 144 4.70e-24 89.4
MS.gene39454.t1 AT1G32540 54.930 71 32 0 1 71 107 177 4.99e-24 90.1
MS.gene39454.t1 AT1G32540 57.576 66 28 0 1 66 74 139 2.61e-22 85.1
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 46 131 1.85e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 46 131 1.85e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 46 131 1.85e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 46 131 1.85e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 46 131 1.85e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 51 136 2.08e-21 83.6
MS.gene39454.t1 AT4G20380 46.512 86 37 1 1 77 51 136 2.08e-21 83.6
MS.gene39454.t1 AT4G20380 47.436 78 32 1 1 69 46 123 2.04e-19 76.6
MS.gene39454.t1 AT4G20380 37.662 77 45 1 1 74 5 81 4.70e-13 60.5
MS.gene39454.t1 AT4G20380 36.607 112 36 1 1 77 46 157 3.56e-18 75.5
MS.gene39454.t1 AT4G20380 42.424 66 35 1 1 63 5 70 1.54e-12 60.5

Find 32 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCGGTTTGTGACAGAAAT+CGG 0.319178 4.2:-22194441 MS.gene39454:CDS
AGGCCGCTAACAACCAGATT+TGG 0.328376 4.2:+22193903 None:intergenic
GCATGAACATTTGACATGTT+TGG 0.343047 4.2:+22196697 None:intergenic
TTCCTTCGTCACAGCTGATC+AGG 0.397948 4.2:-22194541 MS.gene39454:intron
GGCCGCTAACAACCAGATTT+GGG 0.413926 4.2:+22193904 None:intergenic
AAACCAACTCCTCCCAAATC+TGG 0.417656 4.2:-22193916 MS.gene39454:CDS
GCCGGTTTGTGACAGAAATC+GGG 0.420532 4.2:-22194440 MS.gene39454:CDS
CTAATACAATGTTGACTGTC+TGG 0.430906 4.2:+22196672 None:intergenic
CATACGGTGCTCCACAAGTT+AGG 0.437213 4.2:-22194473 MS.gene39454:CDS
CAACCAGATTTGGGAGGAGT+TGG 0.440236 4.2:+22193913 None:intergenic
CCCCGATTTCTGTCACAAAC+CGG 0.464843 4.2:+22194439 None:intergenic
ACAGTCAACATTGTATTAGA+AGG 0.509196 4.2:-22196668 MS.gene39454:intron
CATTTGACATGTTTGGCACC+TGG 0.517148 4.2:+22196704 None:intergenic
CGCCTGCTTTCGTATCCACC+AGG 0.522323 4.2:-22196722 MS.gene39454:CDS
AAGTTAGGTGTTCATCATGC+CGG 0.527912 4.2:-22194458 MS.gene39454:CDS
CTAACTTGTGGAGCACCGTA+TGG 0.535574 4.2:+22194474 None:intergenic
CAGACAACAAGCGGCCTCCA+TGG 0.537061 4.2:-22193954 MS.gene39454:CDS
CACCTGGTGGATACGAAAGC+AGG 0.543193 4.2:+22196720 None:intergenic
CTCCCAAATCTGGTTGTTAG+CGG 0.560296 4.2:-22193906 None:intergenic
TTGTGCAGGCAGACAACAAG+CGG 0.571247 4.2:-22193963 MS.gene39454:intron
TAACTTGTGGAGCACCGTAT+GGG 0.583052 4.2:+22194475 None:intergenic
TTACTTTGTTGTACAGACCA+TGG 0.588700 4.2:+22193937 None:intergenic
TTCGTCACAGCTGATCAGGT+TGG 0.596338 4.2:-22194537 MS.gene39454:intron
CAGGTTGGACAAGTTAAGTG+TGG 0.602861 4.2:-22194522 MS.gene39454:CDS
CATGATGAACACCTAACTTG+TGG 0.604697 4.2:+22194462 None:intergenic
AGGTTGGACAAGTTAAGTGT+GGG 0.628125 4.2:-22194521 MS.gene39454:CDS
TTGACATGTTTGGCACCTGG+TGG 0.642814 4.2:+22196707 None:intergenic
GTATTGCTAATGTACCCATA+CGG 0.650598 4.2:-22194489 MS.gene39454:CDS
CCGGTTTGTGACAGAAATCG+GGG 0.660382 4.2:-22194439 MS.gene39454:intron
CGCTAACAACCAGATTTGGG+AGG 0.672253 4.2:+22193907 None:intergenic
CTGGTGGATACGAAAGCAGG+CGG 0.684251 4.2:+22196723 None:intergenic
CTTTGTTGTACAGACCATGG+AGG 0.723748 4.2:+22193940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTGATTTTATATTGT+TGG - chr4.2:22196619-22196638 MS.gene39454:intron 10.0%
!! AAAATGTCTAATTGAAAATT+TGG + chr4.2:22195932-22195951 None:intergenic 15.0%
!!! TTTTGACTTAAAAAATTAGA+AGG + chr4.2:22196284-22196303 None:intergenic 15.0%
!! ACATATTACATATTGTTCTA+AGG - chr4.2:22196338-22196357 MS.gene39454:intron 20.0%
!! AGTAACATTTCTAACAAAAA+AGG - chr4.2:22195823-22195842 MS.gene39454:intron 20.0%
!! ATATTCAACATTGTATTAGA+AGG - chr4.2:22195600-22195619 MS.gene39454:intron 20.0%
!! GAAAATTTCATTAACATAAC+AGG + chr4.2:22195534-22195553 None:intergenic 20.0%
!! TTCATAAACAATTACATGAT+TGG - chr4.2:22195722-22195741 MS.gene39454:intron 20.0%
!! TTCATATTTGAAAATTGTGT+CGG + chr4.2:22195036-22195055 None:intergenic 20.0%
!!! ATAATGACCAAATATTTTCT+TGG - chr4.2:22194877-22194896 MS.gene39454:intron 20.0%
!!! TTCAATTAGACATTTTAGTA+TGG - chr4.2:22195936-22195955 MS.gene39454:intron 20.0%
! AACTTAAAATGCAAGTTTCA+TGG + chr4.2:22195670-22195689 None:intergenic 25.0%
! ATCAAAAGTTGAAGATTACA+TGG - chr4.2:22195108-22195127 MS.gene39454:intron 25.0%
! ATTTGTCCAAAGTACAAAAA+AGG + chr4.2:22194554-22194573 None:intergenic 25.0%
! CTTTAAATTATCCAAGTGTA+GGG - chr4.2:22196509-22196528 MS.gene39454:intron 25.0%
! TACATAAATCAACTTGCAAT+TGG + chr4.2:22196319-22196338 None:intergenic 25.0%
! TACTTGTACAAAATATGACA+TGG - chr4.2:22196047-22196066 MS.gene39454:intron 25.0%
! TCAAAAGTTGAAGATTACAT+GGG - chr4.2:22195109-22195128 MS.gene39454:intron 25.0%
! TCATCAAATTCAAATGTGAT+TGG + chr4.2:22195894-22195913 None:intergenic 25.0%
! TTTAAATTATCCAAGTGTAG+GGG - chr4.2:22196510-22196529 MS.gene39454:intron 25.0%
! TTTGTCCAAAGTACAAAAAA+GGG + chr4.2:22194553-22194572 None:intergenic 25.0%
!! ATGAAATTTTCAATGCTCTA+CGG - chr4.2:22194358-22194377 MS.gene39454:intron 25.0%
!!! TTTCTAAAAGCTAATTTTCG+AGG + chr4.2:22194097-22194116 None:intergenic 25.0%
AACAACTATTTCAACATCGA+TGG - chr4.2:22194729-22194748 MS.gene39454:intron 30.0%
AACTTCCTGGTCATTATAAA+AGG - chr4.2:22195846-22195865 MS.gene39454:intron 30.0%
ACAGTCAACATTGTATTAGA+AGG - chr4.2:22193984-22194003 MS.gene39454:intron 30.0%
ACTTCCTGGTCATTATAAAA+GGG - chr4.2:22195847-22195866 MS.gene39454:intron 30.0%
AGACATGTTCATGAGAAAAA+AGG + chr4.2:22194018-22194037 None:intergenic 30.0%
AGCTAACATGTATAAGATCA+TGG - chr4.2:22195385-22195404 MS.gene39454:intron 30.0%
CAGGAAATTTGATTACAATC+AGG + chr4.2:22195515-22195534 None:intergenic 30.0%
CCTACACTTGGATAATTTAA+AGG + chr4.2:22196511-22196530 None:intergenic 30.0%
CCTTTAAATTATCCAAGTGT+AGG - chr4.2:22196508-22196527 MS.gene39454:intron 30.0%
CTCAAGTTTATATCTCACAT+TGG - chr4.2:22194481-22194500 MS.gene39454:CDS 30.0%
TAGTTGATCTACGAAGATTT+GGG + chr4.2:22194265-22194284 None:intergenic 30.0%
TCTTCAGATTGAATGAATAG+AGG + chr4.2:22196588-22196607 None:intergenic 30.0%
TGAGGATATCAGTGTTTAAA+TGG + chr4.2:22196663-22196682 None:intergenic 30.0%
TGCAGCTTCAATTTCATTAT+GGG + chr4.2:22195147-22195166 None:intergenic 30.0%
TGCGATTCAGTTCAATAAAT+CGG + chr4.2:22194338-22194357 None:intergenic 30.0%
TTAAATTATCCAAGTGTAGG+GGG - chr4.2:22196511-22196530 MS.gene39454:intron 30.0%
TTGCAGCTTCAATTTCATTA+TGG + chr4.2:22195148-22195167 None:intergenic 30.0%
TTGTCCAAAGTACAAAAAAG+GGG + chr4.2:22194552-22194571 None:intergenic 30.0%
! AAACTATCCTCTTTTGTCAA+TGG + chr4.2:22195362-22195381 None:intergenic 30.0%
! ATTCAAATGTGATTGGTCAT+TGG + chr4.2:22195887-22195906 None:intergenic 30.0%
! TGAAATTTTCAATGCTCTAC+GGG - chr4.2:22194359-22194378 MS.gene39454:intron 30.0%
!! AGTTCAGGTTTTTATACATC+TGG - chr4.2:22195686-22195705 MS.gene39454:intron 30.0%
!!! AGTCTAGTTTTCTCTTTGTA+CGG - chr4.2:22196446-22196465 MS.gene39454:intron 30.0%
!!! GAAACTTGCATTTTAAGTTC+AGG - chr4.2:22195671-22195690 MS.gene39454:intron 30.0%
AAAGAGTGGTTAAGTTCCAT+TGG - chr4.2:22194424-22194443 MS.gene39454:intron 35.0%
AAAGTTGAAGATTACATGGG+AGG - chr4.2:22195112-22195131 MS.gene39454:intron 35.0%
AACAACTCGTTGATCATAGT+TGG + chr4.2:22195084-22195103 None:intergenic 35.0%
AACTATGATCAACGAGTTGT+TGG - chr4.2:22195083-22195102 MS.gene39454:intron 35.0%
AAGTTTGACACTTGATGTAG+CGG - chr4.2:22196378-22196397 MS.gene39454:intron 35.0%
AATGATTGACCAAGAACTCA+TGG - chr4.2:22195301-22195320 MS.gene39454:intron 35.0%
ACAATCAGGCGTTAAACAAA+AGG + chr4.2:22195501-22195520 None:intergenic 35.0%
ACAGGCTACTCCTAAAATTA+GGG + chr4.2:22194946-22194965 None:intergenic 35.0%
ACATAGCGTATATGCATTCT+TGG + chr4.2:22195971-22195990 None:intergenic 35.0%
ACATTGTTTGGATATTGCTG+TGG + chr4.2:22195002-22195021 None:intergenic 35.0%
ACTACGCTCATGTAATTAGA+AGG - chr4.2:22195417-22195436 MS.gene39454:intron 35.0%
AGAAGATGAGAACTATAACC+AGG - chr4.2:22194905-22194924 MS.gene39454:intron 35.0%
CATTGTTTGGATATTGCTGT+GGG + chr4.2:22195001-22195020 None:intergenic 35.0%
CGATTCAGTTCAATAAATCG+GGG + chr4.2:22194336-22194355 None:intergenic 35.0%
CTAACAAAAAAGGAACTTCC+TGG - chr4.2:22195833-22195852 MS.gene39454:intron 35.0%
CTACAACAGATGAACCATAT+CGG - chr4.2:22194282-22194301 MS.gene39454:intron 35.0%
CTGGGAACCAAGAAAATATT+TGG + chr4.2:22194887-22194906 None:intergenic 35.0%
GACAAATTTGCAGTTGAGAT+AGG - chr4.2:22194567-22194586 MS.gene39454:intron 35.0%
GCAAATGATTGGAATGAGAA+TGG + chr4.2:22194679-22194698 None:intergenic 35.0%
GCATGAACATTTGACATGTT+TGG + chr4.2:22193958-22193977 None:intergenic 35.0%
GCGATTCAGTTCAATAAATC+GGG + chr4.2:22194337-22194356 None:intergenic 35.0%
GGTTATAGTTCTCATCTTCT+GGG + chr4.2:22194905-22194924 None:intergenic 35.0%
GTAGTTGATCTACGAAGATT+TGG + chr4.2:22194266-22194285 None:intergenic 35.0%
GTATTGCTAATGTACCCATA+CGG - chr4.2:22196163-22196182 MS.gene39454:intron 35.0%
GTGAGATATAAACTTGAGAG+AGG + chr4.2:22194479-22194498 None:intergenic 35.0%
TACAGGCTACTCCTAAAATT+AGG + chr4.2:22194947-22194966 None:intergenic 35.0%
TATCATCTTGATCTTCGTCT+TGG + chr4.2:22195333-22195352 None:intergenic 35.0%
TCAATAAATCGGGGATTGTT+GGG + chr4.2:22194327-22194346 None:intergenic 35.0%
TCAGATTGAATGAATAGAGG+CGG + chr4.2:22196585-22196604 None:intergenic 35.0%
TGAAATAGTTGTTACCGTCT+AGG + chr4.2:22194722-22194741 None:intergenic 35.0%
TGGTTATAGTTCTCATCTTC+TGG + chr4.2:22194906-22194925 None:intergenic 35.0%
TTCAATAAATCGGGGATTGT+TGG + chr4.2:22194328-22194347 None:intergenic 35.0%
TTTATTTGAGAATCTCCAGC+TGG - chr4.2:22194069-22194088 MS.gene39454:intron 35.0%
! CATGGCATTATTCTTTTGCA+TGG - chr4.2:22196065-22196084 MS.gene39454:intron 35.0%
! CTAATACAATGTTGACTGTC+TGG + chr4.2:22193983-22194002 None:intergenic 35.0%
! GTATCCCTTTTATAATGACC+AGG + chr4.2:22195854-22195873 None:intergenic 35.0%
! TATCCTCATGATTTTTGTGC+AGG - chr4.2:22196675-22196694 MS.gene39454:CDS 35.0%
! TTACTTTGTTGTACAGACCA+TGG + chr4.2:22196718-22196737 None:intergenic 35.0%
!! TTGTATACTCAGCTTTAAGC+TGG + chr4.2:22195199-22195218 None:intergenic 35.0%
AAGTTAGGTGTTCATCATGC+CGG - chr4.2:22196194-22196213 MS.gene39454:intron 40.0%
AATAGTTGTTACCGTCTAGG+TGG + chr4.2:22194719-22194738 None:intergenic 40.0%
ACCTATTCCAATGACGTTCT+AGG - chr4.2:22194828-22194847 MS.gene39454:intron 40.0%
AGCACACAACTACCAATAAC+AGG + chr4.2:22195757-22195776 None:intergenic 40.0%
AGGTTGGACAAGTTAAGTGT+GGG - chr4.2:22196131-22196150 MS.gene39454:intron 40.0%
ATAGTTGGAGTAGAACTGAG+CGG + chr4.2:22195069-22195088 None:intergenic 40.0%
ATCTACGAAGATTTGGGTAG+TGG + chr4.2:22194259-22194278 None:intergenic 40.0%
CAGATTGAATGAATAGAGGC+GGG + chr4.2:22196584-22196603 None:intergenic 40.0%
CATGATGAACACCTAACTTG+TGG + chr4.2:22196193-22196212 None:intergenic 40.0%
CCACTTTGACATAAAGTGGA+GGG + chr4.2:22194394-22194413 None:intergenic 40.0%
CCTCCACTTTATGTCAAAGT+GGG - chr4.2:22194392-22194411 MS.gene39454:intron 40.0%
GAATGGTGAATGATCCGATA+TGG + chr4.2:22194299-22194318 None:intergenic 40.0%
TATAACCCTGATACCATGTC+AGG - chr4.2:22194153-22194172 MS.gene39454:intron 40.0%
TCCTAGAACGTCATTGGAAT+AGG + chr4.2:22194832-22194851 None:intergenic 40.0%
TGTCCCACTTTGACATAAAG+TGG + chr4.2:22194398-22194417 None:intergenic 40.0%
TGTTTATGCCTCAAACAGAG+AGG - chr4.2:22194512-22194531 MS.gene39454:CDS 40.0%
! ACATAAAGTGGAGGGTGTAA+AGG + chr4.2:22194386-22194405 None:intergenic 40.0%
! ATCAACCTGGCCCTAATTTT+AGG - chr4.2:22194933-22194952 MS.gene39454:intron 40.0%
! CTATCCTCTTTTGTCAATGG+CGG + chr4.2:22195359-22195378 None:intergenic 40.0%
! TATCCTCTTTTGTCAATGGC+GGG + chr4.2:22195358-22195377 None:intergenic 40.0%
! TTGAAAATTGTGTCGGCGTT+AGG + chr4.2:22195029-22195048 None:intergenic 40.0%
!! ATTCCAATGACGTTCTAGGA+CGG - chr4.2:22194832-22194851 MS.gene39454:intron 40.0%
!! CTTGAGAGCATTGAGCAATA+GGG + chr4.2:22194625-22194644 None:intergenic 40.0%
!! GTGGGACATTTTGAAAAGAG+TGG - chr4.2:22194410-22194429 MS.gene39454:intron 40.0%
!! TCATCAGCCATTTGCTGTTT+GGG + chr4.2:22194806-22194825 None:intergenic 40.0%
!! TTCATCAGCCATTTGCTGTT+TGG + chr4.2:22194807-22194826 None:intergenic 40.0%
!!! GCTTCCCCTTTTTTGTACTT+TGG - chr4.2:22194545-22194564 MS.gene39454:intron 40.0%
AAACCAACTCCTCCCAAATC+TGG - chr4.2:22196736-22196755 MS.gene39454:CDS 45.0%
AATGGGGTGGTGCAAATGAT+TGG + chr4.2:22194690-22194709 None:intergenic 45.0%
ACAGCTTATGAGTTGCAGTG+CGG + chr4.2:22195457-22195476 None:intergenic 45.0%
ATCATCAATCCCCCTACACT+TGG + chr4.2:22196523-22196542 None:intergenic 45.0%
ATCGGATCATTCACCATTCG+TGG - chr4.2:22194300-22194319 MS.gene39454:intron 45.0%
ATCTTCATCCAGTCCTGACA+TGG + chr4.2:22194169-22194188 None:intergenic 45.0%
ATTGAATGAATAGAGGCGGG+TGG + chr4.2:22196581-22196600 None:intergenic 45.0%
CATTTGACATGTTTGGCACC+TGG + chr4.2:22193951-22193970 None:intergenic 45.0%
CCCACTTTGACATAAAGTGG+AGG + chr4.2:22194395-22194414 None:intergenic 45.0%
CCCTCCACTTTATGTCAAAG+TGG - chr4.2:22194391-22194410 MS.gene39454:intron 45.0%
CGCTGAAGAGTTCAGTATTG+AGG + chr4.2:22194761-22194780 None:intergenic 45.0%
CGTCTAGGTGGTTCCAAAAT+GGG + chr4.2:22194707-22194726 None:intergenic 45.0%
CTACTCCTAAAATTAGGGCC+AGG + chr4.2:22194941-22194960 None:intergenic 45.0%
CTGCCTGCACAAAAATCATG+AGG + chr4.2:22196681-22196700 None:intergenic 45.0%
GCGTATATGCATTCTTGGAG+AGG + chr4.2:22195966-22195985 None:intergenic 45.0%
GTAGTGGTCCTTGAAAAGGA+CGG + chr4.2:22194243-22194262 None:intergenic 45.0%
GTCTAGGTGGTTCCAAAATG+GGG + chr4.2:22194706-22194725 None:intergenic 45.0%
GTGGGATATCATCATCGCTT+GGG + chr4.2:22194983-22195002 None:intergenic 45.0%
TAACCAGGATGACATCAACC+TGG - chr4.2:22194920-22194939 MS.gene39454:intron 45.0%
TAGTTGGAGTAGAACTGAGC+GGG + chr4.2:22195068-22195087 None:intergenic 45.0%
TGACTGACCCAAACAGCAAA+TGG - chr4.2:22194796-22194815 MS.gene39454:intron 45.0%
TGCCAGTCAAGTAGCCAAAA+TGG + chr4.2:22194858-22194877 None:intergenic 45.0%
TGGACGAACAGTCCTGTTAT+TGG - chr4.2:22195742-22195761 MS.gene39454:intron 45.0%
TGGGATATCATCATCGCTTG+GGG + chr4.2:22194982-22195001 None:intergenic 45.0%
TGTGGGATATCATCATCGCT+TGG + chr4.2:22194984-22195003 None:intergenic 45.0%
TTGAATGAATAGAGGCGGGT+GGG + chr4.2:22196580-22196599 None:intergenic 45.0%
TTGGTCAATCATTTCGCCAG+TGG + chr4.2:22195294-22195313 None:intergenic 45.0%
! ATAACTGGGGTGTTACCTTC+AGG + chr4.2:22194198-22194217 None:intergenic 45.0%
! ATTGGTAGTTGTGTGCTTGC+TGG - chr4.2:22195760-22195779 MS.gene39454:intron 45.0%
! CAGGTTGGACAAGTTAAGTG+TGG - chr4.2:22196130-22196149 MS.gene39454:intron 45.0%
! CTTTGTTGTACAGACCATGG+AGG + chr4.2:22196715-22196734 None:intergenic 45.0%
! TAACTGGGGTGTTACCTTCA+GGG + chr4.2:22194197-22194216 None:intergenic 45.0%
! TCATTTGCACCACCCCATTT+TGG - chr4.2:22194691-22194710 MS.gene39454:intron 45.0%
! TGGCTGGAGCTTTGATAACT+GGG + chr4.2:22194212-22194231 None:intergenic 45.0%
! TTGGGTAGTGGTCCTTGAAA+AGG + chr4.2:22194247-22194266 None:intergenic 45.0%
!! CTAATTTTCGAGGAACCAGC+TGG + chr4.2:22194087-22194106 None:intergenic 45.0%
!! GCTTGAGAGCATTGAGCAAT+AGG + chr4.2:22194626-22194645 None:intergenic 45.0%
!! TAACTTGTGGAGCACCGTAT+GGG + chr4.2:22196180-22196199 None:intergenic 45.0%
!! TGCCGGTTTGTGACAGAAAT+CGG - chr4.2:22196211-22196230 MS.gene39454:intron 45.0%
!!! CCATTTTGGAACCACCTAGA+CGG - chr4.2:22194705-22194724 MS.gene39454:intron 45.0%
AACCTGATCAGCTGTGACGA+AGG + chr4.2:22196116-22196135 None:intergenic 50.0%
AGACCCGCCATTGACAAAAG+AGG - chr4.2:22195352-22195371 MS.gene39454:intron 50.0%
CAACCAGATTTGGGAGGAGT+TGG + chr4.2:22196742-22196761 None:intergenic 50.0%
CAACTCAAGACCTGCTGCAA+TGG - chr4.2:22195226-22195245 MS.gene39454:intron 50.0%
CAGCTTATGAGTTGCAGTGC+GGG + chr4.2:22195456-22195475 None:intergenic 50.0%
CATACGGTGCTCCACAAGTT+AGG - chr4.2:22196179-22196198 MS.gene39454:intron 50.0%
CCAGTCCTGACATGGTATCA+GGG + chr4.2:22194161-22194180 None:intergenic 50.0%
CCCCGATTTCTGTCACAAAC+CGG + chr4.2:22196216-22196235 None:intergenic 50.0%
CCGTCTAGGTGGTTCCAAAA+TGG + chr4.2:22194708-22194727 None:intergenic 50.0%
GATGAAGATACGACCCCTGA+AGG - chr4.2:22194180-22194199 MS.gene39454:intron 50.0%
GCGTTAGGCACGACATTGTT+TGG + chr4.2:22195014-22195033 None:intergenic 50.0%
GGAATGAGAATGGAGCTGTC+AGG + chr4.2:22194669-22194688 None:intergenic 50.0%
GTATTTAGCCTCCTCAGCAG+TGG + chr4.2:22194457-22194476 None:intergenic 50.0%
TAGGTGGTTCCAAAATGGGG+TGG + chr4.2:22194703-22194722 None:intergenic 50.0%
TATCATCATCGCTTGGGGCT+AGG + chr4.2:22194977-22194996 None:intergenic 50.0%
TCAATGCTCTCAAGCCGACT+CGG - chr4.2:22194631-22194650 MS.gene39454:intron 50.0%
TCCAGTCCTGACATGGTATC+AGG + chr4.2:22194162-22194181 None:intergenic 50.0%
TGGCATGAGCCATGAGTTCT+TGG + chr4.2:22195313-22195332 None:intergenic 50.0%
TTCCTTCGTCACAGCTGATC+AGG - chr4.2:22196111-22196130 MS.gene39454:intron 50.0%
TTCGTCACAGCTGATCAGGT+TGG - chr4.2:22196115-22196134 MS.gene39454:intron 50.0%
TTGACATGTTTGGCACCTGG+TGG + chr4.2:22193948-22193967 None:intergenic 50.0%
TTGTGCAGGCAGACAACAAG+CGG - chr4.2:22196689-22196708 MS.gene39454:CDS 50.0%
! AACTGGGGTGTTACCTTCAG+GGG + chr4.2:22194196-22194215 None:intergenic 50.0%
! CCGGTTTGTGACAGAAATCG+GGG - chr4.2:22196213-22196232 MS.gene39454:intron 50.0%
! CTGGCTGGAGCTTTGATAAC+TGG + chr4.2:22194213-22194232 None:intergenic 50.0%
! GGCTGGAGCTTTGATAACTG+GGG + chr4.2:22194211-22194230 None:intergenic 50.0%
!! CTAACTTGTGGAGCACCGTA+TGG + chr4.2:22196181-22196200 None:intergenic 50.0%
!! GAGCATTGAGCAATAGGGTG+AGG + chr4.2:22194620-22194639 None:intergenic 50.0%
!! GCCGGTTTGTGACAGAAATC+GGG - chr4.2:22196212-22196231 MS.gene39454:intron 50.0%
!!! ACGTTCTAGGACGGCCATTT+TGG - chr4.2:22194841-22194860 MS.gene39454:intron 50.0%
!!! GGCCATTTTGGCTACTTGAC+TGG - chr4.2:22194853-22194872 MS.gene39454:intron 50.0%
AACGGCTTGTCCTGAGCCTT+CGG + chr4.2:22195258-22195277 None:intergenic 55.0%
AGTGGCGCGAAATGGGACAA+CGG + chr4.2:22195276-22195295 None:intergenic 55.0%
ATGGATACAGCCGAAGGCTC+AGG - chr4.2:22195245-22195264 MS.gene39454:intron 55.0%
ATTTCGCCAGTGGCGCGAAA+TGG + chr4.2:22195284-22195303 None:intergenic 55.0%
CCCTGATACCATGTCAGGAC+TGG - chr4.2:22194158-22194177 MS.gene39454:intron 55.0%
CCTCAAACAGAGAGGCTTGC+AGG - chr4.2:22194520-22194539 MS.gene39454:CDS 55.0%
CCTGCAAGCCTCTCTGTTTG+AGG + chr4.2:22194523-22194542 None:intergenic 55.0%
GCTGCAATGGATACAGCCGA+AGG - chr4.2:22195239-22195258 MS.gene39454:intron 55.0%
GGGATTGTTGGGTCCACGAA+TGG + chr4.2:22194316-22194335 None:intergenic 55.0%
GTGGTCCTTGAAAAGGACGG+AGG + chr4.2:22194240-22194259 None:intergenic 55.0%
TCGGCTGTATCCATTGCAGC+AGG + chr4.2:22195239-22195258 None:intergenic 55.0%
TGAAAAGGACGGAGGTCTGC+TGG + chr4.2:22194232-22194251 None:intergenic 55.0%
TGGCCGTCCTAGAACGTCAT+TGG + chr4.2:22194838-22194857 None:intergenic 55.0%
TTGGAACTCCACCACTGCTG+AGG - chr4.2:22194443-22194462 MS.gene39454:CDS 55.0%
TTTAGCCTCCTCAGCAGTGG+TGG + chr4.2:22194454-22194473 None:intergenic 55.0%
TTTCGCCAGTGGCGCGAAAT+GGG + chr4.2:22195283-22195302 None:intergenic 55.0%
! ACTCTTCAGCGCACTGATGC+AGG - chr4.2:22194770-22194789 MS.gene39454:intron 55.0%
! CAATGCTCTCAAGCCGACTC+GGG - chr4.2:22194632-22194651 MS.gene39454:intron 55.0%
! CACCTGGTGGATACGAAAGC+AGG + chr4.2:22193935-22193954 None:intergenic 55.0%
! CAGCAGTGGTGGAGTTCCAA+TGG + chr4.2:22194443-22194462 None:intergenic 55.0%
! CTGGTGGATACGAAAGCAGG+CGG + chr4.2:22193932-22193951 None:intergenic 55.0%
! GCAGACCTCCGTCCTTTTCA+AGG - chr4.2:22194232-22194251 MS.gene39454:intron 55.0%
CAGACAACAAGCGGCCTCCA+TGG - chr4.2:22196698-22196717 MS.gene39454:CDS 60.0%
CGCCTGCTTTCGTATCCACC+AGG - chr4.2:22193930-22193949 MS.gene39454:CDS 60.0%
GAACTCCACCACTGCTGAGG+AGG - chr4.2:22194446-22194465 MS.gene39454:CDS 60.0%
GTTGTCCCATTTCGCGCCAC+TGG - chr4.2:22195275-22195294 MS.gene39454:intron 60.0%
TCATCGCTTGGGGCTAGGTG+TGG + chr4.2:22194972-22194991 None:intergenic 60.0%
TGGGGCTAGGTGTGGAGTAC+AGG + chr4.2:22194964-22194983 None:intergenic 60.0%
!! GGGCCAGGTTGATGTCATCC+TGG + chr4.2:22194926-22194945 None:intergenic 60.0%
AAGGACGGAGGTCTGCTGGC+TGG + chr4.2:22194228-22194247 None:intergenic 65.0%
GGCGTTCCAGTGACCCGAGT+CGG + chr4.2:22194648-22194667 None:intergenic 65.0%
!! CTCAAGCCGACTCGGGTCAC+TGG - chr4.2:22194639-22194658 MS.gene39454:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22193909 22196765 22193909 ID=MS.gene39454
chr4.2 mRNA 22193909 22196765 22193909 ID=MS.gene39454.t1;Parent=MS.gene39454
chr4.2 exon 22196669 22196765 22196669 ID=MS.gene39454.t1.exon1;Parent=MS.gene39454.t1
chr4.2 CDS 22196669 22196765 22196669 ID=cds.MS.gene39454.t1;Parent=MS.gene39454.t1
chr4.2 exon 22194440 22194549 22194440 ID=MS.gene39454.t1.exon2;Parent=MS.gene39454.t1
chr4.2 CDS 22194440 22194549 22194440 ID=cds.MS.gene39454.t1;Parent=MS.gene39454.t1
chr4.2 exon 22193909 22193977 22193909 ID=MS.gene39454.t1.exon3;Parent=MS.gene39454.t1
chr4.2 CDS 22193909 22193977 22193909 ID=cds.MS.gene39454.t1;Parent=MS.gene39454.t1
Gene Sequence

>MS.gene39454

ATGATTTGTGGTTCTTGTCGCCGCCTGCTTTCGTATCCACCAGGTGCCAAACATGTCAAATGTTCATGCTGCCAGACAGTCAACATTGTATTAGAAGGTCAGTTCTCCTTTTTTCTCATGAACATGTCTATGCTGTGAAACTTTGCATTTTAAGTTCAATTTTATTTGAGAATCTCCAGCTGGTTCCTCGAAAATTAGCTTTTAGAAATTCGATTAAGAAGACTATATCGTACACGTTTTAGAGTATAACCCTGATACCATGTCAGGACTGGATGAAGATACGACCCCTGAAGGTAACACCCCAGTTATCAAAGCTCCAGCCAGCAGACCTCCGTCCTTTTCAAGGACCACTACCCAAATCTTCGTAGATCAACTACAACAGATGAACCATATCGGATCATTCACCATTCGTGGACCCAACAATCCCCGATTTATTGAACTGAATCGCAATGAAATTTTCAATGCTCTACGGGACCTTTACACCCTCCACTTTATGTCAAAGTGGGACATTTTGAAAAGAGTGGTTAAGTTCCATTGGAACTCCACCACTGCTGAGGAGGCTAAATACCTCTCTCAAGTTTATATCTCACATTGGTTCTTAGATGTTTATGCCTCAAACAGAGAGGCTTGCAGGAAGCTTCCCCTTTTTTGTACTTTGGACAAATTTGCAGTTGAGATAGGTATTCAATCAACAATTTATGATAATTACCTCACCCTATTGCTCAATGCTCTCAAGCCGACTCGGGTCACTGGAACGCCTGACAGCTCCATTCTCATTCCAATCATTTGCACCACCCCATTTTGGAACCACCTAGACGGTAACAACTATTTCAACATCGATGGATTTATCCTCAATACTGAACTCTTCAGCGCACTGATGCAGGTCATGACTGACCCAAACAGCAAATGGCTGATGAACACCTATTCCAATGACGTTCTAGGACGGCCATTTTGGCTACTTGACTGGCATAATGACCAAATATTTTCTTGGTTCCCAGAAGATGAGAACTATAACCAGGATGACATCAACCTGGCCCTAATTTTAGGAGTAGCCTGTACTCCACACCTAGCCCCAAGCGATGATGATATCCCACAGCAATATCCAAACAATGTCGTGCCTAACGCCGACACAATTTTCAAATATGAACGAAGAATTCCCGCTCAGTTCTACTCCAACTATGATCAACGAGTTGTTGGTCATCAAAAGTTGAAGATTACATGGGAGGAAATGCTAACCCATAATGAAATTGAAGCTGCAAGACAGAAGAATGAAGAAGAATACAATATCCAGCTTAAAGCTGAGTATACAAAGCTCGTCAACTCAAGACCTGCTGCAATGGATACAGCCGAAGGCTCAGGACAAGCCGTTGTCCCATTTCGCGCCACTGGCGAAATGATTGACCAAGAACTCATGGCTCATGCCAAGACGAAGATCAAGATGATAGACCCGCCATTGACAAAAGAGGATAGTTTTGTAGCTAACATGTATAAGATCATGGACTATAAGTACTACGCTCATGTAATTAGAAGGATTGACACTAACACCCGCACTGCAACTCATAAGCTGTTGATCTATAACGATTAGACTACCTTTTGTTTAACGCCTGATTGTAATCAAATTTCCTGTTATGTTAATGAAATTTTCTTTCAGTTTTATTTTATGCATCTGATTGAGTAAATGCTTTCATAACATATTCAACATTGTATTAGAAGGTCACTTATCTTTCTTTGCTTATTTTTCTCGTGAAAACGTATTTGCCATGAAACTTGCATTTTAAGTTCAGGTTTTTATACATCTGGTTGATTAATTACTTTCATAAACAATTACATGATTGGACGAACAGTCCTGTTATTGGTAGTTGTGTGCTTGCTGGTGCAAACAATCTTGTGTATTTAGTAAATTTTAATTTTAATAGTAACATTTCTAACAAAAAAGGAACTTCCTGGTCATTATAAAAGGGATACAGTCTAAGCACCAATGACCAATCACATTTGAATTTGATGATGCTATTATCATGCTCCAAATTTTCAATTAGACATTTTAGTATGGTAAACCTCTCCAAGAATGCATATACGCTATGTACTATTTGAAGTTTTTTCACTTGTCTAACTATATTCTATTATTTTGAAGTTCACTATACTTGTACAAAATATGACATGGCATTATTCTTTTGCATGGCAAGCTACTTATATCTTAACTCATTCCTTCGTCACAGCTGATCAGGTTGGACAAGTTAAGTGTGGGAGTTGTGCAGTATTGCTAATGTACCCATACGGTGCTCCACAAGTTAGGTGTTCATCATGCCGGTTTGTGACAGAAATCGGGGTATGTAAACTGTTGACATTCACTAGTTCTCTTTATACATGTTTTACCTTCTAATTTTTTAAGTCAAAATAAACAATGTAACCAATTGCAAGTTGATTTATGTACATATTACATATTGTTCTAAGGTTTACTGACATATCATTAAGTTTGACACTTGATGTAGCGGATCTGATCTATACATATATATTATTACATTATATTCAACTATTTAAGTCTAGTTTTCTCTTTGTACGGTGTACTTCGTGCACTCGAAATAAAACAACTCATTTCAAACCTTTAAATTATCCAAGTGTAGGGGGATTGATGATGCTTTACTTTTGTGAATAATTGAAGACATTAAGTCCCACCCGCCTCTATTCATTCAATCTGAAGACAAACTGTAGCTATTATTGATTTTATATTGTTGGCTTAAACAACTGATGTTTCCATTTAAACACTGATATCCTCATGATTTTTGTGCAGGCAGACAACAAGCGGCCTCCATGGTCTGTACAACAAAGTAAACCAACTCCTCCCAAATCTGGTTGTTAG
Protein sequence

>MS.gene39454.t1

MICGSCRRLLSYPPGAKHVKCSCCQTVNIVLEADQVGQVKCGSCAVLLMYPYGAPQVRCSSCRFVTEIGADNKRPPWSVQQSKPTPPKSGC