AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39488

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39488.t1 MTR_4g088580 93.906 361 6 5 1 350 29 384 0.0 671
MS.gene39488.t1 MTR_2g014170 42.693 349 127 17 9 350 27 309 6.09e-45 156
MS.gene39488.t1 MTR_8g068210 62.651 83 31 0 17 99 23 105 2.21e-34 129
MS.gene39488.t1 MTR_8g479350 65.432 81 28 0 21 101 63 143 4.61e-33 125
MS.gene39488.t1 MTR_2g096740 76.562 64 15 0 26 89 62 125 7.11e-33 124
MS.gene39488.t1 MTR_2g096740 76.562 64 15 0 26 89 77 140 8.41e-33 124
MS.gene39488.t1 MTR_4g063780 62.222 90 30 1 19 104 68 157 1.53e-32 124
MS.gene39488.t1 MTR_4g022370 48.276 116 60 0 19 134 75 190 2.25e-32 124
MS.gene39488.t1 MTR_2g093220 83.333 60 10 0 21 80 35 94 2.33e-32 122
MS.gene39488.t1 MTR_8g027295 53.333 105 39 1 15 119 13 107 2.37e-32 122
MS.gene39488.t1 MTR_7g059400 72.222 72 14 1 9 80 65 130 3.09e-32 124
MS.gene39488.t1 MTR_4g461080 85.965 57 8 0 22 78 25 81 5.93e-32 121
MS.gene39488.t1 MTR_3g091820 71.233 73 16 1 13 80 25 97 1.52e-31 120
MS.gene39488.t1 MTR_8g079060 65.789 76 26 0 22 97 25 100 2.03e-31 118
MS.gene39488.t1 MTR_3g090430 69.444 72 22 0 11 82 36 107 2.13e-30 118
MS.gene39488.t1 MTR_2g013370 66.667 78 19 2 11 82 25 101 2.75e-30 117
MS.gene39488.t1 MTR_2g014060 77.049 61 14 0 17 77 16 76 3.05e-30 118
MS.gene39488.t1 MTR_4g089095 72.973 74 19 1 10 83 34 106 1.09e-29 115
MS.gene39488.t1 MTR_4g089095 80.000 60 11 1 24 83 14 72 1.52e-29 114
MS.gene39488.t1 MTR_5g031440 79.630 54 11 0 23 76 40 93 2.10e-29 115
MS.gene39488.t1 MTR_1g077600 67.606 71 21 1 12 80 27 97 3.47e-29 114
MS.gene39488.t1 MTR_1g077600 67.606 71 21 1 12 80 27 97 3.83e-29 114
MS.gene39488.t1 MTR_1g056810 84.906 53 8 0 28 80 14 66 3.83e-29 114
MS.gene39488.t1 MTR_8g015840 78.571 56 12 0 22 77 16 71 7.23e-29 111
MS.gene39488.t1 MTR_4g109980 81.132 53 10 0 24 76 38 90 1.53e-28 113
MS.gene39488.t1 MTR_3g077750 72.131 61 17 0 16 76 51 111 1.91e-28 113
MS.gene39488.t1 MTR_2g059540 84.000 50 8 0 25 74 30 79 8.70e-27 105
MS.gene39488.t1 MTR_3g435480 65.625 64 22 0 16 79 126 189 1.71e-26 109
MS.gene39488.t1 MTR_4g082060 62.121 66 25 0 16 81 112 177 5.41e-26 108
MS.gene39488.t1 MTR_7g086780 63.077 65 24 0 17 81 90 154 1.01e-25 107
MS.gene39488.t1 MTR_7g010950 65.079 63 22 0 17 79 126 188 1.47e-25 107
MS.gene39488.t1 MTR_6g012450 67.213 61 20 0 19 79 129 189 5.64e-25 105
MS.gene39488.t1 MTR_2g016030 50.000 94 38 2 16 109 37 121 1.54e-24 98.2
MS.gene39488.t1 MTR_6g027450 56.923 65 28 0 17 81 80 144 2.15e-24 102
MS.gene39488.t1 MTR_8g044220 56.923 65 28 0 16 80 108 172 2.86e-24 103
MS.gene39488.t1 MTR_6g027460 58.462 65 27 0 17 81 87 151 3.79e-24 102
MS.gene39488.t1 MTR_5g041420 63.333 60 22 0 20 79 132 191 4.10e-23 98.6
MS.gene39488.t1 MTR_5g041530 64.407 59 21 0 21 79 135 193 2.35e-21 94.4
MS.gene39488.t1 MTR_5g041380 67.857 56 18 0 24 79 125 180 4.24e-21 93.6
MS.gene39488.t1 MTR_5g041400 70.370 54 16 0 26 79 119 172 5.99e-21 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39488.t1 AT1G28310 37.500 336 148 16 26 350 27 311 2.64e-37 136
MS.gene39488.t1 AT1G28310 37.500 336 148 16 26 350 51 335 3.16e-37 137
MS.gene39488.t1 AT5G65590 71.053 76 22 0 12 87 27 102 4.07e-35 130
MS.gene39488.t1 AT5G60200 81.967 61 11 0 20 80 49 109 3.67e-34 126
MS.gene39488.t1 AT2G37590 65.753 73 25 0 10 82 73 145 1.57e-32 124
MS.gene39488.t1 AT3G61850 45.455 143 59 4 23 146 71 213 4.02e-32 122
MS.gene39488.t1 AT3G61850 44.828 145 61 4 21 146 57 201 4.27e-32 122
MS.gene39488.t1 AT3G61850 44.828 145 61 4 21 146 57 201 4.27e-32 122
MS.gene39488.t1 AT5G02460 82.759 58 10 0 23 80 92 149 1.19e-31 123
MS.gene39488.t1 AT3G55370 66.667 75 25 0 8 82 58 132 2.74e-31 120
MS.gene39488.t1 AT2G28810 79.310 58 12 0 20 77 71 128 3.40e-31 120
MS.gene39488.t1 AT5G60850 70.667 75 19 1 24 95 49 123 3.45e-31 120
MS.gene39488.t1 AT1G21340 64.557 79 25 1 11 86 20 98 3.62e-31 119
MS.gene39488.t1 AT3G50410 63.218 87 28 3 1 83 1 87 3.63e-31 118
MS.gene39488.t1 AT3G55370 66.667 75 25 0 8 82 58 132 3.95e-31 120
MS.gene39488.t1 AT1G07640 46.032 126 64 1 7 132 2 123 4.44e-31 119
MS.gene39488.t1 AT2G28810 79.310 58 12 0 20 77 88 145 4.63e-31 120
MS.gene39488.t1 AT3G55370 66.667 75 25 0 8 82 103 177 5.06e-31 120
MS.gene39488.t1 AT3G45610 77.586 58 13 0 23 80 37 94 7.43e-31 117
MS.gene39488.t1 AT2G28510 82.456 57 10 0 24 80 46 102 9.26e-31 118
MS.gene39488.t1 AT1G07640 60.000 85 29 1 1 80 47 131 1.04e-30 119
MS.gene39488.t1 AT1G07640 60.000 85 29 1 1 80 55 139 1.27e-30 119
MS.gene39488.t1 AT3G61850 53.271 107 41 2 21 118 57 163 1.80e-30 118
MS.gene39488.t1 AT3G61850 54.286 105 39 2 23 118 71 175 1.81e-30 118
MS.gene39488.t1 AT1G51700 76.923 65 12 2 17 80 26 88 1.85e-30 115
MS.gene39488.t1 AT3G52440 81.818 55 10 0 28 82 27 81 1.98e-29 114
MS.gene39488.t1 AT3G52440 81.818 55 10 0 28 82 46 100 2.91e-29 114
MS.gene39488.t1 AT3G21270 84.906 53 8 0 26 78 29 81 4.81e-29 111
MS.gene39488.t1 AT4G24060 75.000 60 15 0 21 80 48 107 5.02e-29 115
MS.gene39488.t1 AT4G00940 74.603 63 16 0 23 85 65 127 5.39e-29 114
MS.gene39488.t1 AT4G00940 74.603 63 16 0 23 85 65 127 5.39e-29 114
MS.gene39488.t1 AT4G00940 74.603 63 16 0 23 85 65 127 5.39e-29 114
MS.gene39488.t1 AT4G21050 66.197 71 24 0 28 98 26 96 1.37e-28 110
MS.gene39488.t1 AT5G66940 56.977 86 33 2 22 104 28 112 1.67e-28 110
MS.gene39488.t1 AT4G38000 79.310 58 11 1 23 79 38 95 5.73e-28 110
MS.gene39488.t1 AT5G62940 69.355 62 19 0 15 76 62 123 3.49e-27 110
MS.gene39488.t1 AT3G47500 66.667 66 22 0 14 79 98 163 1.71e-26 109
MS.gene39488.t1 AT5G62430 67.213 61 20 0 19 79 47 107 1.17e-25 105
MS.gene39488.t1 AT1G64620 74.545 55 14 0 21 75 44 98 1.92e-25 105
MS.gene39488.t1 AT2G46590 78.431 51 11 0 23 73 65 115 2.55e-25 105
MS.gene39488.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.78e-25 106
MS.gene39488.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.78e-25 106
MS.gene39488.t1 AT5G39660 68.852 61 19 0 19 79 131 191 2.78e-25 106
MS.gene39488.t1 AT2G46590 78.431 51 11 0 23 73 77 127 3.02e-25 105
MS.gene39488.t1 AT1G26790 63.934 61 22 0 19 79 104 164 1.61e-24 103
MS.gene39488.t1 AT1G69570 60.294 68 27 0 19 86 125 192 2.10e-24 103
MS.gene39488.t1 AT1G47655 72.549 51 14 0 24 74 27 77 6.63e-24 98.2
MS.gene39488.t1 AT1G29160 56.338 71 29 1 11 79 45 115 1.45e-23 96.3
MS.gene39488.t1 AT4G21040 57.143 77 30 2 28 103 27 101 2.21e-23 97.4
MS.gene39488.t1 AT2G34140 63.333 60 22 0 20 79 52 111 2.62e-23 95.5
MS.gene39488.t1 AT4G21080 65.517 58 19 1 28 84 27 84 2.19e-21 92.0
MS.gene39488.t1 AT4G21030 46.053 76 41 0 28 103 23 98 2.84e-14 71.2

Find 68 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCAATTCTCTTCTTTA+TGG 0.060643 4.2:-22540892 MS.gene39488:CDS
CAGATTCTTCTTCTCTTTAT+TGG 0.115189 4.2:-22540357 MS.gene39488:CDS
AACAGCAACAACAAGCTTTA+AGG 0.128070 4.2:-22541215 MS.gene39488:CDS
CTTCTTCAAGTGGTGGAAAT+AGG 0.186986 4.2:-22541269 MS.gene39488:CDS
GAATTATGACCAATATTATT+AGG 0.234994 4.2:+22540262 None:intergenic
CAGGTGAATGGTCTTGGATT+AGG 0.278693 4.2:-22540760 MS.gene39488:CDS
CATAAAGAAGAGAATTGATA+TGG 0.297144 4.2:+22540893 None:intergenic
GCAAAGCCTGTAAAAGATAT+TGG 0.299579 4.2:-22541116 MS.gene39488:CDS
AACAACTACAGTTCCATCTT+TGG 0.305663 4.2:-22540670 MS.gene39488:CDS
TCATCATGAGGCTGGGATAA+TGG 0.306350 4.2:-22540497 MS.gene39488:CDS
TGCTGCAGTGTTGTTGTTGT+TGG 0.309230 4.2:+22540321 None:intergenic
GATGATTACAGAAATGGATT+TGG 0.345610 4.2:-22540703 MS.gene39488:CDS
CATCATGATGATTACAGAAA+TGG 0.360895 4.2:-22540709 MS.gene39488:CDS
CAACAGGTTTGAGTAGTGTT+TGG 0.362182 4.2:-22540300 MS.gene39488:CDS
AGAGTTTCAAGTTCTTCTTC+TGG 0.369826 4.2:-22540811 MS.gene39488:CDS
TTCTTCAAGTGGTGGAAATA+GGG 0.372187 4.2:-22541268 MS.gene39488:CDS
GCTGCTGCTGGTGGTGGTGG+TGG 0.374632 4.2:-22540541 MS.gene39488:CDS
TTAAGTAGAGAAGCCATAAC+TGG 0.397152 4.2:+22540601 None:intergenic
GCTGCTGCTGCTGGTGGTGG+TGG 0.403043 4.2:-22540544 MS.gene39488:CDS
CAGCCTCAGGTGAATGGTCT+TGG 0.403606 4.2:-22540766 MS.gene39488:CDS
ACAGCTGCTGCTGCTGGTGG+TGG 0.407708 4.2:-22540547 MS.gene39488:CDS
AAGAGAATTGATATGGTTTG+AGG 0.411504 4.2:+22540900 None:intergenic
GATCACCAACCTAATAATAT+TGG 0.414706 4.2:-22540271 MS.gene39488:CDS
TAGAAGCAATATCAAAACTA+TGG 0.419426 4.2:+22540923 None:intergenic
ATGTTTGAAGAAGAGTGATT+TGG 0.420876 4.2:+22541024 None:intergenic
GGTGGTGGTGGTGGAATGAA+AGG 0.420899 4.2:-22540532 MS.gene39488:CDS
AACAACAACACTGCAGCAAC+AGG 0.445401 4.2:-22540316 MS.gene39488:CDS
AAGTGGTGGAAATAGGGTTA+TGG 0.446908 4.2:-22541262 MS.gene39488:CDS
ACAAACCCATGTTCTCCATC+TGG 0.452753 4.2:+22540380 None:intergenic
TAGGTGAAGGACTACAAAAT+AGG 0.461358 4.2:-22540456 MS.gene39488:CDS
GGCCTCTACTACTCCAGTTA+TGG 0.463925 4.2:-22540614 MS.gene39488:CDS
GGGTTTGAATTAGCACTAGT+AGG 0.464115 4.2:+22540967 None:intergenic
GAATTGAACCTTGTTGCAAA+AGG 0.464424 4.2:+22540838 None:intergenic
TATGACCAATATTATTAGGT+TGG 0.469646 4.2:+22540266 None:intergenic
TTTCTGCAACCACCACCAAC+AGG 0.472277 4.2:+22541066 None:intergenic
ATGAGTACAGCTGCTGCTGC+TGG 0.476216 4.2:-22540553 MS.gene39488:CDS
CAGGCTTTGCAGAAGTGTCT+AGG 0.477081 4.2:+22541129 None:intergenic
ATCCCAGCCTCATGATGAAA+AGG 0.481394 4.2:+22540502 None:intergenic
TCAAAACCAGATGGAGAACA+TGG 0.510931 4.2:-22540386 MS.gene39488:CDS
GTTGTTGTTGTTGAGAAGGA+GGG 0.513508 4.2:+22540947 None:intergenic
CAGCAGCAGCCTCAGGTGAA+TGG 0.518167 4.2:-22540772 MS.gene39488:CDS
CTTAGAAACGTTCCTGTTGG+TGG 0.520182 4.2:-22541078 MS.gene39488:CDS
AGTAATAATATGAATGCTAA+TGG 0.525718 4.2:-22540421 MS.gene39488:CDS
AACTGGAGTAGTAGAGGCCA+TGG 0.527816 4.2:+22540618 None:intergenic
TGTTGTTGTTGTTGAGAAGG+AGG 0.530854 4.2:+22540946 None:intergenic
TGAAGGACTACAAAATAGGT+TGG 0.535498 4.2:-22540452 MS.gene39488:CDS
AGTAGAAGAAGAACCAAAGA+TGG 0.540629 4.2:+22540657 None:intergenic
TGGTGTTGTTGTTGTTGAGA+AGG 0.544856 4.2:+22540943 None:intergenic
CTAGTAGGAGTTGAAGAAGA+AGG 0.548358 4.2:+22540982 None:intergenic
TCTTCAGCAGCAGCAGCCTC+AGG 0.551095 4.2:-22540779 MS.gene39488:CDS
TGTAAAAGATATTGGACAAG+AGG 0.561250 4.2:-22541108 MS.gene39488:CDS
ATTTCAATGGCTTCTTCAAG+TGG 0.564116 4.2:-22541279 None:intergenic
TCAATGGCTTCTTCAAGTGG+TGG 0.566820 4.2:-22541276 MS.gene39488:CDS
AGCCATAACTGGAGTAGTAG+AGG 0.573379 4.2:+22540612 None:intergenic
AGTACAGCTGCTGCTGCTGG+TGG 0.574327 4.2:-22540550 MS.gene39488:CDS
AGAAACGTTCCTGTTGGTGG+TGG 0.578028 4.2:-22541075 MS.gene39488:CDS
TGTTGAAGAAGCTCTTGACC+TGG 0.579160 4.2:+22541234 None:intergenic
TACAAAATAGGTTGGATCAG+TGG 0.582074 4.2:-22540444 MS.gene39488:CDS
AAAAGATATTGGACAAGAGG+TGG 0.582761 4.2:-22541105 MS.gene39488:CDS
GATTCAAAAGAAGTGAAACT+AGG 0.595250 4.2:-22540475 MS.gene39488:CDS
CAAAACCAGATGGAGAACAT+GGG 0.602211 4.2:-22540385 MS.gene39488:CDS
GGTGGTGGTGGAATGAAAGG+TGG 0.606003 4.2:-22540529 MS.gene39488:CDS
TTCAACTAATGATAACTCCA+TGG 0.607734 4.2:-22540635 MS.gene39488:CDS
ACACTTAGAAACGTTCCTGT+TGG 0.611337 4.2:-22541081 MS.gene39488:CDS
AAAGAAGTGAAACTAGGTGA+AGG 0.626336 4.2:-22540469 MS.gene39488:CDS
AATAATATGAATGCTAATGG+TGG 0.635173 4.2:-22540418 MS.gene39488:CDS
AATCCAAGACCATTCACCTG+AGG 0.720277 4.2:+22540763 None:intergenic
GTGTTTGATGAATCACAACG+TGG 0.749662 4.2:+22541189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATAATATGAATGCTAA+TGG - chr4.2:22541095-22541114 MS.gene39488:CDS 20.0%
!! GAATTATGACCAATATTATT+AGG + chr4.2:22541257-22541276 None:intergenic 20.0%
! AATAATATGAATGCTAATGG+TGG - chr4.2:22541098-22541117 MS.gene39488:CDS 25.0%
! CATAAAGAAGAGAATTGATA+TGG + chr4.2:22540626-22540645 None:intergenic 25.0%
! CATATCAATTCTCTTCTTTA+TGG - chr4.2:22540624-22540643 MS.gene39488:CDS 25.0%
! TAGAAGCAATATCAAAACTA+TGG + chr4.2:22540596-22540615 None:intergenic 25.0%
! TATGACCAATATTATTAGGT+TGG + chr4.2:22541253-22541272 None:intergenic 25.0%
AAGAGAATTGATATGGTTTG+AGG + chr4.2:22540619-22540638 None:intergenic 30.0%
ATGTTTGAAGAAGAGTGATT+TGG + chr4.2:22540495-22540514 None:intergenic 30.0%
CAGATTCTTCTTCTCTTTAT+TGG - chr4.2:22541159-22541178 MS.gene39488:CDS 30.0%
CATCATGATGATTACAGAAA+TGG - chr4.2:22540807-22540826 MS.gene39488:CDS 30.0%
GATCACCAACCTAATAATAT+TGG - chr4.2:22541245-22541264 MS.gene39488:CDS 30.0%
GATTCAAAAGAAGTGAAACT+AGG - chr4.2:22541041-22541060 MS.gene39488:CDS 30.0%
TGTAAAAGATATTGGACAAG+AGG - chr4.2:22540408-22540427 MS.gene39488:CDS 30.0%
TTCAACTAATGATAACTCCA+TGG - chr4.2:22540881-22540900 MS.gene39488:CDS 30.0%
! GATGATTACAGAAATGGATT+TGG - chr4.2:22540813-22540832 MS.gene39488:CDS 30.0%
! TCTTGTCCAATATCTTTTAC+AGG + chr4.2:22540409-22540428 None:intergenic 30.0%
!! TTGGATTAGGTTTTTCATCT+GGG - chr4.2:22540769-22540788 MS.gene39488:CDS 30.0%
AAAAGATATTGGACAAGAGG+TGG - chr4.2:22540411-22540430 MS.gene39488:CDS 35.0%
AAAGAAGTGAAACTAGGTGA+AGG - chr4.2:22541047-22541066 MS.gene39488:CDS 35.0%
AACAACTACAGTTCCATCTT+TGG - chr4.2:22540846-22540865 MS.gene39488:CDS 35.0%
AACAGCAACAACAAGCTTTA+AGG - chr4.2:22540301-22540320 MS.gene39488:CDS 35.0%
AATAGGGTTATGGAAAAACC+AGG - chr4.2:22540264-22540283 MS.gene39488:CDS 35.0%
AGTAGAAGAAGAACCAAAGA+TGG + chr4.2:22540862-22540881 None:intergenic 35.0%
GAATTGAACCTTGTTGCAAA+AGG + chr4.2:22540681-22540700 None:intergenic 35.0%
GCAAAGCCTGTAAAAGATAT+TGG - chr4.2:22540400-22540419 MS.gene39488:CDS 35.0%
TAGGTGAAGGACTACAAAAT+AGG - chr4.2:22541060-22541079 MS.gene39488:CDS 35.0%
TGAAGGACTACAAAATAGGT+TGG - chr4.2:22541064-22541083 MS.gene39488:CDS 35.0%
TTAAGTAGAGAAGCCATAAC+TGG + chr4.2:22540918-22540937 None:intergenic 35.0%
! TACAAAATAGGTTGGATCAG+TGG - chr4.2:22541072-22541091 MS.gene39488:CDS 35.0%
! TGAACTTTCCTTTTGCAACA+AGG - chr4.2:22540670-22540689 MS.gene39488:CDS 35.0%
! TTCTTCAAGTGGTGGAAATA+GGG - chr4.2:22540248-22540267 MS.gene39488:CDS 35.0%
!! AGAGTTTCAAGTTCTTCTTC+TGG - chr4.2:22540705-22540724 MS.gene39488:CDS 35.0%
!! CTTGGATTAGGTTTTTCATC+TGG - chr4.2:22540768-22540787 MS.gene39488:CDS 35.0%
!! TGGATTAGGTTTTTCATCTG+GGG - chr4.2:22540770-22540789 MS.gene39488:CDS 35.0%
!!! TGTTGCTTTTCAAAACCAGA+TGG - chr4.2:22541121-22541140 MS.gene39488:CDS 35.0%
ACACTTAGAAACGTTCCTGT+TGG - chr4.2:22540435-22540454 MS.gene39488:CDS 40.0%
CAAAACCAGATGGAGAACAT+GGG - chr4.2:22541131-22541150 MS.gene39488:CDS 40.0%
CAACAGGTTTGAGTAGTGTT+TGG - chr4.2:22541216-22541235 MS.gene39488:CDS 40.0%
CTAGTAGGAGTTGAAGAAGA+AGG + chr4.2:22540537-22540556 None:intergenic 40.0%
GGGTTTGAATTAGCACTAGT+AGG + chr4.2:22540552-22540571 None:intergenic 40.0%
GTGTTTGATGAATCACAACG+TGG + chr4.2:22540330-22540349 None:intergenic 40.0%
TCAAAACCAGATGGAGAACA+TGG - chr4.2:22541130-22541149 MS.gene39488:CDS 40.0%
! AAGTGGTGGAAATAGGGTTA+TGG - chr4.2:22540254-22540273 MS.gene39488:CDS 40.0%
! ATCCTTTTCATCATGAGGCT+GGG - chr4.2:22541012-22541031 MS.gene39488:CDS 40.0%
! CTTCTTCAAGTGGTGGAAAT+AGG - chr4.2:22540247-22540266 MS.gene39488:CDS 40.0%
! GTTGTTGTTGTTGAGAAGGA+GGG + chr4.2:22540572-22540591 None:intergenic 40.0%
! TGGTGATCCTTTTCATCATG+AGG - chr4.2:22541007-22541026 MS.gene39488:CDS 40.0%
! TGTTGTTGTTGTTGAGAAGG+AGG + chr4.2:22540573-22540592 None:intergenic 40.0%
!! TGGTGTTGTTGTTGTTGAGA+AGG + chr4.2:22540576-22540595 None:intergenic 40.0%
AACAACAACACTGCAGCAAC+AGG - chr4.2:22541200-22541219 MS.gene39488:CDS 45.0%
AATCCAAGACCATTCACCTG+AGG + chr4.2:22540756-22540775 None:intergenic 45.0%
ACAAACCCATGTTCTCCATC+TGG + chr4.2:22541139-22541158 None:intergenic 45.0%
AGCCATAACTGGAGTAGTAG+AGG + chr4.2:22540907-22540926 None:intergenic 45.0%
ATCCCAGCCTCATGATGAAA+AGG + chr4.2:22541017-22541036 None:intergenic 45.0%
CTTAGAAACGTTCCTGTTGG+TGG - chr4.2:22540438-22540457 MS.gene39488:CDS 45.0%
TCATCATGAGGCTGGGATAA+TGG - chr4.2:22541019-22541038 MS.gene39488:CDS 45.0%
TGTTGAAGAAGCTCTTGACC+TGG + chr4.2:22540285-22540304 None:intergenic 45.0%
! CAGGTGAATGGTCTTGGATT+AGG - chr4.2:22540756-22540775 MS.gene39488:CDS 45.0%
! GATCCTTTTCATCATGAGGC+TGG - chr4.2:22541011-22541030 MS.gene39488:CDS 45.0%
! TGCTGCAGTGTTGTTGTTGT+TGG + chr4.2:22541198-22541217 None:intergenic 45.0%
AACTGGAGTAGTAGAGGCCA+TGG + chr4.2:22540901-22540920 None:intergenic 50.0%
GGCCTCTACTACTCCAGTTA+TGG - chr4.2:22540902-22540921 MS.gene39488:CDS 50.0%
TTTCTGCAACCACCACCAAC+AGG + chr4.2:22540453-22540472 None:intergenic 50.0%
! AGAAACGTTCCTGTTGGTGG+TGG - chr4.2:22540441-22540460 MS.gene39488:CDS 50.0%
!! CAGGCTTTGCAGAAGTGTCT+AGG + chr4.2:22540390-22540409 None:intergenic 50.0%
ATGAGTACAGCTGCTGCTGC+TGG - chr4.2:22540963-22540982 MS.gene39488:CDS 55.0%
CAGCCTCAGGTGAATGGTCT+TGG - chr4.2:22540750-22540769 MS.gene39488:CDS 55.0%
!! GGTGGTGGTGGAATGAAAGG+TGG - chr4.2:22540987-22541006 MS.gene39488:CDS 55.0%
!! GGTGGTGGTGGTGGAATGAA+AGG - chr4.2:22540984-22541003 MS.gene39488:CDS 55.0%
AGTACAGCTGCTGCTGCTGG+TGG - chr4.2:22540966-22540985 MS.gene39488:CDS 60.0%
CAGCAGCAGCCTCAGGTGAA+TGG - chr4.2:22540744-22540763 MS.gene39488:CDS 60.0%
TCTTCAGCAGCAGCAGCCTC+AGG - chr4.2:22540737-22540756 MS.gene39488:CDS 60.0%
ACAGCTGCTGCTGCTGGTGG+TGG - chr4.2:22540969-22540988 MS.gene39488:CDS 65.0%
!! GCTGCTGCTGCTGGTGGTGG+TGG - chr4.2:22540972-22540991 MS.gene39488:CDS 70.0%
!! GCTGCTGCTGGTGGTGGTGG+TGG - chr4.2:22540975-22540994 MS.gene39488:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22540243 22541295 22540243 ID=MS.gene39488
chr4.2 mRNA 22540243 22541295 22540243 ID=MS.gene39488.t1;Parent=MS.gene39488
chr4.2 exon 22540243 22541295 22540243 ID=MS.gene39488.t1.exon1;Parent=MS.gene39488.t1
chr4.2 CDS 22540243 22541295 22540243 ID=cds.MS.gene39488.t1;Parent=MS.gene39488.t1
Gene Sequence

>MS.gene39488

ATGGCTTCTTCAAGTGGTGGAAATAGGGTTATGGAAAAACCAGGTCAAGAGCTTCTTCAACAGCAACAACAAGCTTTAAGGTGTCCACGTTGTGATTCATCAAACACTAAATTCTGTTACTACAATAACTACAGTTTATCACAACCTAGACACTTCTGCAAAGCCTGTAAAAGATATTGGACAAGAGGTGGAACACTTAGAAACGTTCCTGTTGGTGGTGGTTGCAGAAAAAACAAACGTCTTAAACGACCAAATCACTCTTCTTCAAACATTGATACTGCTACTGCTTCTCCTTCTTCTTCAACTCCTACTAGTGCTAATTCAAACCCTCCTTCTCAACAACAACAACACCATAGTTTTGATATTGCTTCTACCTCAAACCATATCAATTCTCTTCTTTATGGTAACTCTTCTTGTCATGATGTGATGAACTTTCCTTTTGCAACAAGGTTCAATTCTACTAGAGTTTCAAGTTCTTCTTCTGGTTATGATCATCTTCAGCAGCAGCAGCCTCAGGTGAATGGTCTTGGATTAGGTTTTTCATCTGGGGTAGTTAATCTTAATCATCATGATGATTACAGAAATGGATTTGGTTCAAATAATAACAACTACAGTTCCATCTTTGGTTCTTCTTCTACTTCAACTAATGATAACTCCATGGCCTCTACTACTCCAGTTATGGCTTCTCTACTTAACTCTACACTACTACAACAGAAATTCATGAGTACAGCTGCTGCTGCTGGTGGTGGTGGTGGAATGAAAGGTGGTGATCCTTTTCATCATGAGGCTGGGATAATGGATTCAAAAGAAGTGAAACTAGGTGAAGGACTACAAAATAGGTTGGATCAGTGGAGTAATAATATGAATGCTAATGGTGGTGTTGCTTTTCAAAACCAGATGGAGAACATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACAACACCAACAACAACAACACTGCAGCAACAGGTTTGAGTAGTGTTTGGAGTATTGATCACCAACCTAATAATATTGGTCATAATTCAGTTACTTCTCTGATCTAG
Protein sequence

>MS.gene39488.t1

MASSSGGNRVMEKPGQELLQQQQQALRCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGCRKNKRLKRPNHSSSNIDTATASPSSSTPTSANSNPPSQQQQHHSFDIASTSNHINSLLYGNSSCHDVMNFPFATRFNSTRVSSSSSGYDHLQQQQPQVNGLGLGFSSGVVNLNHHDDYRNGFGSNNNNYSSIFGSSSTSTNDNSMASTTPVMASLLNSTLLQQKFMSTAAAAGGGGGMKGGDPFHHEAGIMDSKEVKLGEGLQNRLDQWSNNMNANGGVAFQNQMENMGLSDSSSLYWNNNNTNNNNTAATGLSSVWSIDHQPNNIGHNSVTSLI