AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39544

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39544.t1 MTR_4g088070 79.699 399 54 3 1 399 1 372 0.0 606
MS.gene39544.t1 MTR_7g025010 58.374 406 147 6 8 399 7 404 1.20e-150 439
MS.gene39544.t1 MTR_4g088080 70.871 333 80 3 1 333 1 316 6.56e-148 422
MS.gene39544.t1 MTR_2g015000 38.018 434 200 17 8 403 7 409 3.15e-59 199
MS.gene39544.t1 MTR_3g462480 65.753 73 25 0 332 404 22 94 2.49e-26 102
MS.gene39544.t1 MTR_6g027390 65.000 60 21 0 35 94 24 83 3.29e-21 93.6
MS.gene39544.t1 MTR_7g086940 69.231 52 16 0 41 92 26 77 1.12e-18 85.9
MS.gene39544.t1 MTR_4g088065 46.903 113 45 4 52 163 134 232 3.39e-18 84.0
MS.gene39544.t1 MTR_4g084550 46.269 67 36 0 37 103 58 124 5.34e-17 79.0
MS.gene39544.t1 MTR_5g081990 37.778 90 50 1 14 103 10 93 9.84e-16 75.5
MS.gene39544.t1 MTR_8g107210 49.180 61 31 0 43 103 96 156 2.25e-15 77.4
MS.gene39544.t1 MTR_4g063735 30.508 177 91 3 43 189 25 199 2.30e-14 73.2
MS.gene39544.t1 MTR_1g019130 40.449 89 43 1 15 103 72 150 4.08e-14 71.6
MS.gene39544.t1 MTR_5g021930 41.975 81 45 2 43 122 30 109 6.95e-14 72.4
MS.gene39544.t1 MTR_1g019130 40.449 89 43 1 15 103 72 150 1.12e-13 70.5
MS.gene39544.t1 MTR_3g115620 33.010 103 62 1 41 143 80 175 8.04e-13 67.8
MS.gene39544.t1 MTR_7g060630 43.333 60 34 0 43 102 8 67 2.68e-12 65.9
MS.gene39544.t1 MTR_1g115315 41.270 63 37 0 40 102 82 144 1.42e-11 64.7
MS.gene39544.t1 MTR_1g115315 41.270 63 37 0 40 102 82 144 1.80e-11 64.7
MS.gene39544.t1 MTR_0777s0020 47.191 89 44 2 76 163 50 136 8.84e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39544.t1 AT5G45980 48.031 127 50 2 9 119 8 134 1.69e-30 120
MS.gene39544.t1 AT3G03660 48.936 94 38 2 1 94 1 84 2.53e-20 90.5
MS.gene39544.t1 AT3G03660 48.936 94 38 2 1 94 1 84 3.59e-20 90.5
MS.gene39544.t1 AT5G17810 68.519 54 17 0 41 94 19 72 5.17e-20 89.7
MS.gene39544.t1 AT5G17810 68.519 54 17 0 41 94 19 72 5.46e-20 89.4
MS.gene39544.t1 AT5G17810 68.519 54 17 0 41 94 19 72 5.46e-20 89.4
MS.gene39544.t1 AT3G03660 48.936 94 38 2 1 94 1 84 6.61e-20 89.7
MS.gene39544.t1 AT3G03660 48.936 94 38 2 1 94 1 84 9.02e-20 90.1
MS.gene39544.t1 AT3G18010 47.541 61 32 0 43 103 76 136 5.86e-15 76.3
MS.gene39544.t1 AT1G46480 47.826 69 35 1 35 103 83 150 2.11e-14 71.6
MS.gene39544.t1 AT3G11260 44.262 61 34 0 43 103 24 84 7.08e-14 70.1
MS.gene39544.t1 AT1G46480 47.826 69 35 1 35 103 83 150 8.25e-14 71.2
MS.gene39544.t1 AT5G05770 43.333 60 34 0 43 102 29 88 1.07e-13 67.8
MS.gene39544.t1 AT5G59340 26.111 180 123 4 43 216 14 189 1.54e-13 70.9
MS.gene39544.t1 AT2G17950 50.000 62 30 1 43 103 38 99 1.65e-13 71.2
MS.gene39544.t1 AT2G33880 45.946 74 36 1 327 400 309 378 2.13e-13 71.6
MS.gene39544.t1 AT2G33880 44.595 74 37 1 327 400 309 378 2.50e-13 71.2
MS.gene39544.t1 AT2G01500 49.091 55 28 0 43 97 61 115 5.22e-13 69.3
MS.gene39544.t1 AT2G28610 42.424 66 38 0 37 102 2 67 2.81e-12 66.6
MS.gene39544.t1 AT4G35550 42.857 63 36 0 41 103 97 159 4.13e-12 66.6
MS.gene39544.t1 AT1G20700 41.270 63 37 0 41 103 31 93 1.19e-11 62.8
MS.gene39544.t1 AT1G20700 36.486 74 47 0 30 103 82 155 1.39e-11 63.9
MS.gene39544.t1 AT1G20700 36.486 74 47 0 30 103 82 155 1.54e-11 63.9

Find 101 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGAACAATTAGAAGAATT+TGG 0.130042 4.2:-23233764 MS.gene39544:CDS
CAAGACCCGCAATTTAATTT+TGG 0.177107 4.2:-23233128 MS.gene39544:CDS
TTAGAAGAATTTGGGAAAAT+TGG 0.179693 4.2:-23233755 MS.gene39544:CDS
CCATTGAGAAAGAGGAAAAT+AGG 0.245649 4.2:+23233390 None:intergenic
AAAGGATTCACGAACGTTAA+AGG 0.277504 4.2:+23232730 None:intergenic
AATAAGTATCATCTTGGTTT+TGG 0.279397 4.2:+23233453 None:intergenic
ATGCGGATTTGCTCAGGCTT+TGG 0.283372 4.2:+23233848 None:intergenic
ATGAACAATGAAAATGAAAA+TGG 0.285973 4.2:-23233227 MS.gene39544:CDS
CACTCTCCCATCATTAATAT+TGG 0.292661 4.2:+23233508 None:intergenic
GCCTGAGCAAATCCGCATTC+TGG 0.308743 4.2:-23233843 MS.gene39544:CDS
TGCGGATTTGCTCAGGCTTT+GGG 0.308960 4.2:+23233849 None:intergenic
GAAGTTGATCATCGGTAAAA+AGG 0.313912 4.2:+23233012 None:intergenic
TCAGGCTTTGGGTTCCATCT+TGG 0.322968 4.2:+23233860 None:intergenic
TTTGGGTTCCATCTTGGCCT+TGG 0.327019 4.2:+23233866 None:intergenic
ATCACAATATCTAACAATAT+TGG 0.329123 4.2:-23233536 MS.gene39544:CDS
TTTCATTGGGAGAGGCTCTT+TGG 0.330296 4.2:+23233558 None:intergenic
GGAGAGATTGTGGATGAATT+AGG 0.338412 4.2:+23233479 None:intergenic
GGTGGTGGTGGTGGGATTTG+TGG 0.341213 4.2:+23233611 None:intergenic
GTGGTGGTGGTGGGATTTGT+GGG 0.343259 4.2:+23233612 None:intergenic
GTTAGATATTGTGATTTCAT+TGG 0.354236 4.2:+23233544 None:intergenic
GGTGAGGGTTCAATCATATT+CGG 0.354951 4.2:+23233293 None:intergenic
ATTTGTGGGAGAGAGTTATT+AGG 0.358733 4.2:+23233626 None:intergenic
GTGAACAATTAGAAGAATTT+GGG 0.359582 4.2:-23233763 MS.gene39544:CDS
TTCCATCTTGGCCTTGGTCC+AGG 0.361114 4.2:+23233872 None:intergenic
TGGTTGGAGTTCGACGAATT+TGG 0.370437 4.2:+23234445 None:intergenic
GATGGATTAGGTGGTGGTGG+TGG 0.376558 4.2:+23233602 None:intergenic
AGAGGCTCTTTGGTGATTAG+AGG 0.377467 4.2:+23233568 None:intergenic
AATTAAATTGCGGGTCTTGT+TGG 0.379122 4.2:+23233132 None:intergenic
CTGGAAGCAATCTACAACTC+AGG 0.386585 4.2:-23233824 MS.gene39544:CDS
GAAGATGATGAAGATGGATT+AGG 0.391757 4.2:+23233590 None:intergenic
TGACACCAAAATTAAATTGC+GGG 0.395460 4.2:+23233123 None:intergenic
CCCCATTCATCGGTTAGAAC+CGG 0.398111 4.2:+23232671 None:intergenic
CCCGGTTCTAACCGATGAAT+GGG 0.399463 4.2:-23232672 MS.gene39544:CDS
TTAGATATTGTGATTTCATT+GGG 0.400328 4.2:+23233545 None:intergenic
GTTGTAGATCGGTTTCAGTT+GGG 0.400667 4.2:+23233423 None:intergenic
GATAATGAAGAAAGAAATCC+TGG 0.405944 4.2:-23233890 MS.gene39544:CDS
CATTGAGAAAGAGGAAAATA+GGG 0.407191 4.2:+23233391 None:intergenic
GTGCATGTGCATCATATTCA+TGG 0.415898 4.2:+23233154 None:intergenic
TTAGGAGCTGGATCTGCAAT+TGG 0.417412 4.2:+23233644 None:intergenic
TTTACCGATGATCAACTTCA+AGG 0.420455 4.2:-23233008 MS.gene39544:intron
TCCAGAATGCGGATTTGCTC+AGG 0.421812 4.2:+23233842 None:intergenic
TTTAACGTTCGTGAATCCTT+TGG 0.434959 4.2:-23232728 MS.gene39544:CDS
ATGATTCTCCATTGAGAAAG+AGG 0.435482 4.2:+23233382 None:intergenic
GGAATCTCCAATATTAATGA+TGG 0.437319 4.2:-23233515 MS.gene39544:CDS
GTGAGGGTTCAATCATATTC+GGG 0.442014 4.2:+23233294 None:intergenic
AAGGATTCACGAACGTTAAA+GGG 0.443421 4.2:+23232731 None:intergenic
ATCTTCTTGATTTCATCTAC+AGG 0.443482 4.2:+23233788 None:intergenic
AAAAGGAATGGAATATCAAC+AGG 0.453783 4.2:+23233029 None:intergenic
CCCCGGTTCTAACCGATGAA+TGG 0.455370 4.2:-23232673 MS.gene39544:CDS
AGTTGTAGATCGGTTTCAGT+TGG 0.468112 4.2:+23233422 None:intergenic
CCCATTCATCGGTTAGAACC+GGG 0.470305 4.2:+23232672 None:intergenic
ATGGATTAGGTGGTGGTGGT+GGG 0.472541 4.2:+23233603 None:intergenic
ATGTCAATGGCCGGAGGTAG+AGG 0.473475 4.2:+23233059 None:intergenic
TGTGAGAATAAGTATCATCT+TGG 0.474305 4.2:+23233447 None:intergenic
GGTGACTTTATAAACTTGCT+TGG 0.474802 4.2:+23234466 None:intergenic
ATGGAGAATCATGTTAATGA+AGG 0.479722 4.2:-23233371 MS.gene39544:CDS
TATTGTGATTTCATTGGGAG+AGG 0.498415 4.2:+23233550 None:intergenic
TTGTAGATCGGTTTCAGTTG+GGG 0.501118 4.2:+23233424 None:intergenic
GAAGATGGATTAGGTGGTGG+TGG 0.501572 4.2:+23233599 None:intergenic
GGGAGAGAGTTATTAGGAGC+TGG 0.513692 4.2:+23233632 None:intergenic
ATCCTGGACCAAGGCCAAGA+TGG 0.528279 4.2:-23233874 MS.gene39544:CDS
AACAGGTGCACTCATGTCAA+TGG 0.531199 4.2:+23233046 None:intergenic
GTTAACCTCGATTACTTCGT+TGG 0.544175 4.2:+23232760 None:intergenic
TCCATGGTAGAGTGAGTTGA+GGG 0.553586 4.2:+23232643 None:intergenic
TTCTTGATTTCATCTACAGG+TGG 0.556034 4.2:+23233791 None:intergenic
GAGGGTGACACCCCATTCAT+CGG 0.558082 4.2:+23232661 None:intergenic
TGGAGAATCATGTTAATGAA+GGG 0.558945 4.2:-23233370 MS.gene39544:CDS
TGATCAAGTGAATGTTCAAG+AGG 0.560823 4.2:-23233180 MS.gene39544:CDS
GTTTGACTCCTTCTGTACCC+CGG 0.561060 4.2:-23232690 MS.gene39544:CDS
TTAGAGGAAGATGATGAAGA+TGG 0.564984 4.2:+23233584 None:intergenic
AACCATCAAAATCGTCTGCA+TGG 0.567329 4.2:-23234427 MS.gene39544:intron
GAATCTCCAATATTAATGAT+GGG 0.575519 4.2:-23233514 MS.gene39544:CDS
ATTACCTTGAAGTTGATCAT+CGG 0.577276 4.2:+23233004 None:intergenic
GGTTAGAACCGGGGTACAGA+AGG 0.581477 4.2:+23232682 None:intergenic
CTCCATGGTAGAGTGAGTTG+AGG 0.583077 4.2:+23232642 None:intergenic
TGATCTTGCTCCTCTACCTC+CGG 0.584790 4.2:-23233069 MS.gene39544:CDS
GGTGCACTCATGTCAATGGC+CGG 0.585287 4.2:+23233050 None:intergenic
GTGACACCAAAATTAAATTG+CGG 0.589142 4.2:+23233122 None:intergenic
TGGAAGCAATCTACAACTCA+GGG 0.590445 4.2:-23233823 MS.gene39544:CDS
ATGTTATCGTAATTAAGCAC+AGG 0.591556 4.2:+23233251 None:intergenic
TTTCAAAACCGCAAGTCCAA+AGG 0.599228 4.2:-23233710 MS.gene39544:CDS
CCACGTAATAATCAGCTCCA+TGG 0.601206 4.2:+23232627 None:intergenic
TGTAGATTGCTTCCAGAATG+CGG 0.605667 4.2:+23233831 None:intergenic
GAAGTAATCGAGGTTAACGC+AGG 0.607642 4.2:-23232755 MS.gene39544:CDS
TACTGCCAACGAAGTAATCG+AGG 0.608047 4.2:-23232765 MS.gene39544:CDS
GGAGAATCATGTTAATGAAG+GGG 0.615858 4.2:-23233369 MS.gene39544:CDS
CAGAACTGCTCTATTACCAA+AGG 0.619722 4.2:+23232712 None:intergenic
AATGACATGACAACACTAGA+GGG 0.631034 4.2:-23233338 MS.gene39544:CDS
GATGATGAAGATGGATTAGG+TGG 0.643509 4.2:+23233593 None:intergenic
ACCCTCAACTCACTCTACCA+TGG 0.647606 4.2:-23232644 MS.gene39544:CDS
TAATGACATGACAACACTAG+AGG 0.647700 4.2:-23233339 MS.gene39544:CDS
GATGAAGATGGATTAGGTGG+TGG 0.649565 4.2:+23233596 None:intergenic
CTCGTAAGAAGAAAGTACAT+TGG 0.652355 4.2:-23234489 MS.gene39544:CDS
AAGAAAGAAATCCTGGACCA+AGG 0.658709 4.2:-23233883 MS.gene39544:CDS
CCATGGAGCTGATTATTACG+TGG 0.665604 4.2:-23232627 MS.gene39544:CDS
AAGTAATCGAGGTTAACGCA+GGG 0.673390 4.2:-23232754 MS.gene39544:CDS
ATGACATGACAACACTAGAG+GGG 0.676653 4.2:-23233337 MS.gene39544:CDS
TATTAATGATGGGAGAGTGT+TGG 0.676983 4.2:-23233504 MS.gene39544:CDS
CCGGTTCTAACCGATGAATG+GGG 0.682846 4.2:-23232671 MS.gene39544:CDS
CCATTCATCGGTTAGAACCG+GGG 0.687831 4.2:+23232673 None:intergenic
GCACTCATGTCAATGGCCGG+AGG 0.689498 4.2:+23233053 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAAAAAAAAAGTAGA+AGG + chr4.2:23232714-23232733 None:intergenic 10.0%
!!! ATTTCTAAAACTATTTTTTA+TGG + chr4.2:23233062-23233081 None:intergenic 10.0%
!!! TTTCTAAAACTATTTTTTAT+GGG + chr4.2:23233061-23233080 None:intergenic 10.0%
!!! AAATAGTTGATTTTAGATTT+AGG + chr4.2:23232939-23232958 None:intergenic 15.0%
!!! ATTATTTTTTAATTATCCCA+TGG - chr4.2:23234154-23234173 MS.gene39544:intron 15.0%
!!! ATTTTTTTAAAGCTAAGATT+AGG + chr4.2:23232909-23232928 None:intergenic 15.0%
!! ATCACAATATCTAACAATAT+TGG - chr4.2:23233543-23233562 MS.gene39544:CDS 20.0%
!! ATGAACAATGAAAATGAAAA+TGG - chr4.2:23233852-23233871 MS.gene39544:CDS 20.0%
!! GCTTATAACAAATATTGTTT+AGG - chr4.2:23233162-23233181 MS.gene39544:CDS 20.0%
!! TATAACAAATATTGTTTAGG+TGG - chr4.2:23233165-23233184 MS.gene39544:CDS 20.0%
!! TTAGATATTGTGATTTCATT+GGG + chr4.2:23233537-23233556 None:intergenic 20.0%
!!! AACTATTTGATCTTTTCTAA+TGG - chr4.2:23232951-23232970 MS.gene39544:intron 20.0%
!!! TTAGTTTTTTTTCCATGTTA+GGG - chr4.2:23232831-23232850 MS.gene39544:intron 20.0%
!!! TTTAGTTTTTTTTCCATGTT+AGG - chr4.2:23232830-23232849 MS.gene39544:intron 20.0%
! GAATCTCCAATATTAATGAT+GGG - chr4.2:23233565-23233584 MS.gene39544:CDS 25.0%
! GTGAACAATTAGAAGAATTT+GGG - chr4.2:23233316-23233335 MS.gene39544:CDS 25.0%
! GTTAGATATTGTGATTTCAT+TGG + chr4.2:23233538-23233557 None:intergenic 25.0%
! TTAGAAGAATTTGGGAAAAT+TGG - chr4.2:23233324-23233343 MS.gene39544:CDS 25.0%
!! AATAAGTATCATCTTGGTTT+TGG + chr4.2:23233629-23233648 None:intergenic 25.0%
!! TATAACGATTAGTTGATGTT+TGG - chr4.2:23234244-23234263 MS.gene39544:intron 25.0%
!!! TAGTTTTTTTTCCATGTTAG+GGG - chr4.2:23232832-23232851 MS.gene39544:intron 25.0%
!!! TCGATATAATTTGTTTCGAT+TGG + chr4.2:23232810-23232829 None:intergenic 25.0%
AAAAAGTAGAAGGAGTTGTT+TGG + chr4.2:23232704-23232723 None:intergenic 30.0%
AAAAGGAATGGAATATCAAC+AGG + chr4.2:23234053-23234072 None:intergenic 30.0%
ATCTTCTTGATTTCATCTAC+AGG + chr4.2:23233294-23233313 None:intergenic 30.0%
ATGGAGAATCATGTTAATGA+AGG - chr4.2:23233708-23233727 MS.gene39544:CDS 30.0%
ATGTTATCGTAATTAAGCAC+AGG + chr4.2:23233831-23233850 None:intergenic 30.0%
CACTTGATCAAAAAGATCAT+TGG + chr4.2:23233889-23233908 None:intergenic 30.0%
CATTGAGAAAGAGGAAAATA+GGG + chr4.2:23233691-23233710 None:intergenic 30.0%
CGTGAACAATTAGAAGAATT+TGG - chr4.2:23233315-23233334 MS.gene39544:CDS 30.0%
GATAATGAAGAAAGAAATCC+TGG - chr4.2:23233189-23233208 MS.gene39544:CDS 30.0%
GGAATCTCCAATATTAATGA+TGG - chr4.2:23233564-23233583 MS.gene39544:CDS 30.0%
GTAAACTTTGCATGTTTGAT+CGG - chr4.2:23233140-23233159 MS.gene39544:CDS 30.0%
GTGACACCAAAATTAAATTG+CGG + chr4.2:23233960-23233979 None:intergenic 30.0%
TAGTAGAGATCATGTTTGTT+TGG + chr4.2:23232876-23232895 None:intergenic 30.0%
TGACACCAAAATTAAATTGC+GGG + chr4.2:23233959-23233978 None:intergenic 30.0%
TGGAGAATCATGTTAATGAA+GGG - chr4.2:23233709-23233728 MS.gene39544:CDS 30.0%
TGTGAGAATAAGTATCATCT+TGG + chr4.2:23233635-23233654 None:intergenic 30.0%
TTAGGATTTCAATGCAAGTA+AGG - chr4.2:23233003-23233022 MS.gene39544:intron 30.0%
TTGCTTGAATGTGTGAATTT+AGG + chr4.2:23233032-23233051 None:intergenic 30.0%
! AAAGAGGAAAATAGGGTTTT+AGG + chr4.2:23233684-23233703 None:intergenic 30.0%
! ATTACCTTGAAGTTGATCAT+CGG + chr4.2:23234078-23234097 None:intergenic 30.0%
!!! GGTTGTCTTTTTGATTTCTT+GGG + chr4.2:23233417-23233436 None:intergenic 30.0%
!!! GTTGTCTTTTTGATTTCTTG+GGG + chr4.2:23233416-23233435 None:intergenic 30.0%
!!! TGCTTTTTTGTTTTTGCCTT+TGG + chr4.2:23233388-23233407 None:intergenic 30.0%
!!! TGGTTGTCTTTTTGATTTCT+TGG + chr4.2:23233418-23233437 None:intergenic 30.0%
!!! TTAGTTGATGTTTGGTTTTC+AGG - chr4.2:23234252-23234271 MS.gene39544:intron 30.0%
AAAGGATTCACGAACGTTAA+AGG + chr4.2:23234352-23234371 None:intergenic 35.0%
AAGGATTCACGAACGTTAAA+GGG + chr4.2:23234351-23234370 None:intergenic 35.0%
AATGACATGACAACACTAGA+GGG - chr4.2:23233741-23233760 MS.gene39544:CDS 35.0%
ACCCAAAATTTCAAGCACAA+GGG - chr4.2:23234127-23234146 MS.gene39544:intron 35.0%
ATGATTCTCCATTGAGAAAG+AGG + chr4.2:23233700-23233719 None:intergenic 35.0%
ATTTGTGGGAGAGAGTTATT+AGG + chr4.2:23233456-23233475 None:intergenic 35.0%
CACTCTCCCATCATTAATAT+TGG + chr4.2:23233574-23233593 None:intergenic 35.0%
CCATTGAGAAAGAGGAAAAT+AGG + chr4.2:23233692-23233711 None:intergenic 35.0%
GGAGAATCATGTTAATGAAG+GGG - chr4.2:23233710-23233729 MS.gene39544:CDS 35.0%
TAATGACATGACAACACTAG+AGG - chr4.2:23233740-23233759 MS.gene39544:CDS 35.0%
TATTGTGATTTCATTGGGAG+AGG + chr4.2:23233532-23233551 None:intergenic 35.0%
TGATCAAGTGAATGTTCAAG+AGG - chr4.2:23233899-23233918 MS.gene39544:intron 35.0%
TGATCATCGGTAAAAAGGAA+TGG + chr4.2:23234065-23234084 None:intergenic 35.0%
TGTTTATATGTCCCCTAACA+TGG + chr4.2:23232846-23232865 None:intergenic 35.0%
TTAGAGGAAGATGATGAAGA+TGG + chr4.2:23233498-23233517 None:intergenic 35.0%
TTCTTGATTTCATCTACAGG+TGG + chr4.2:23233291-23233310 None:intergenic 35.0%
TTGCTTGACGGTGTAAATTT+AGG - chr4.2:23232985-23233004 MS.gene39544:intron 35.0%
TTTAACGTTCGTGAATCCTT+TGG - chr4.2:23234351-23234370 MS.gene39544:intron 35.0%
TTTACACCGTCAAGCAAATA+TGG + chr4.2:23232982-23233001 None:intergenic 35.0%
TTTACCGATGATCAACTTCA+AGG - chr4.2:23234071-23234090 MS.gene39544:intron 35.0%
TTTGGAAACCATTGAAGAGA+GGG + chr4.2:23232686-23232705 None:intergenic 35.0%
! AATTAAATTGCGGGTCTTGT+TGG + chr4.2:23233950-23233969 None:intergenic 35.0%
! ACCCTTGTGCTTGAAATTTT+GGG + chr4.2:23234131-23234150 None:intergenic 35.0%
! ATCAACATTTTGTTGAGGTG+AGG + chr4.2:23233805-23233824 None:intergenic 35.0%
! CCTATTTTCCTCTTTCTCAA+TGG - chr4.2:23233689-23233708 MS.gene39544:CDS 35.0%
! CTCGTAAGAAGAAAGTACAT+TGG - chr4.2:23232590-23232609 MS.gene39544:CDS 35.0%
! GAAGATGATGAAGATGGATT+AGG + chr4.2:23233492-23233511 None:intergenic 35.0%
! GAAGTTGATCATCGGTAAAA+AGG + chr4.2:23234070-23234089 None:intergenic 35.0%
! GGAAGATCAACATTTTGTTG+AGG + chr4.2:23233810-23233829 None:intergenic 35.0%
! GGTGACTTTATAAACTTGCT+TGG + chr4.2:23232616-23232635 None:intergenic 35.0%
! TACCCTTGTGCTTGAAATTT+TGG + chr4.2:23234132-23234151 None:intergenic 35.0%
! TATTAATGATGGGAGAGTGT+TGG - chr4.2:23233575-23233594 MS.gene39544:CDS 35.0%
! TCAACATTTTGTTGAGGTGA+GGG + chr4.2:23233804-23233823 None:intergenic 35.0%
!! CAAGACCCGCAATTTAATTT+TGG - chr4.2:23233951-23233970 MS.gene39544:intron 35.0%
!!! CTTTTTGATTTCTTGGGGTA+GGG + chr4.2:23233411-23233430 None:intergenic 35.0%
!!! GATGTTTGGTTTTCAGGTTT+TGG - chr4.2:23234258-23234277 MS.gene39544:intron 35.0%
!!! GGTTTTAGGTAGTTGTAGAT+CGG + chr4.2:23233670-23233689 None:intergenic 35.0%
!!! GTATCATCTTGGTTTTGGTT+CGG + chr4.2:23233624-23233643 None:intergenic 35.0%
!!! TCTTTTTGATTTCTTGGGGT+AGG + chr4.2:23233412-23233431 None:intergenic 35.0%
AACCATCAAAATCGTCTGCA+TGG - chr4.2:23232652-23232671 MS.gene39544:CDS 40.0%
AAGAAAGAAATCCTGGACCA+AGG - chr4.2:23233196-23233215 MS.gene39544:CDS 40.0%
AAGTAATCGAGGTTAACGCA+GGG - chr4.2:23234325-23234344 MS.gene39544:intron 40.0%
ATGACATGACAACACTAGAG+GGG - chr4.2:23233742-23233761 MS.gene39544:CDS 40.0%
CACCCAAAATTTCAAGCACA+AGG - chr4.2:23234126-23234145 MS.gene39544:intron 40.0%
CAGAACTGCTCTATTACCAA+AGG + chr4.2:23234370-23234389 None:intergenic 40.0%
GGAGAGATTGTGGATGAATT+AGG + chr4.2:23233603-23233622 None:intergenic 40.0%
GGTGAGGGTTCAATCATATT+CGG + chr4.2:23233789-23233808 None:intergenic 40.0%
GTCTGACCATATTTGCTTGA+CGG - chr4.2:23232973-23232992 MS.gene39544:intron 40.0%
GTGAGGGTTCAATCATATTC+GGG + chr4.2:23233788-23233807 None:intergenic 40.0%
GTGCATGTGCATCATATTCA+TGG + chr4.2:23233928-23233947 None:intergenic 40.0%
GTTAACCTCGATTACTTCGT+TGG + chr4.2:23234322-23234341 None:intergenic 40.0%
GTTTGGAAACCATTGAAGAG+AGG + chr4.2:23232687-23232706 None:intergenic 40.0%
TGGAAGCAATCTACAACTCA+GGG - chr4.2:23233256-23233275 MS.gene39544:CDS 40.0%
TGTAGATTGCTTCCAGAATG+CGG + chr4.2:23233251-23233270 None:intergenic 40.0%
TTGGAAACCATTGAAGAGAG+GGG + chr4.2:23232685-23232704 None:intergenic 40.0%
TTTCAAAACCGCAAGTCCAA+AGG - chr4.2:23233369-23233388 MS.gene39544:CDS 40.0%
! AGTTGTAGATCGGTTTCAGT+TGG + chr4.2:23233660-23233679 None:intergenic 40.0%
! GTTGTAGATCGGTTTCAGTT+GGG + chr4.2:23233659-23233678 None:intergenic 40.0%
! TACCATGCAGACGATTTTGA+TGG + chr4.2:23232657-23232676 None:intergenic 40.0%
! TTGTAGATCGGTTTCAGTTG+GGG + chr4.2:23233658-23233677 None:intergenic 40.0%
!! ATGCAGACGATTTTGATGGT+TGG + chr4.2:23232653-23232672 None:intergenic 40.0%
!! GATGATGAAGATGGATTAGG+TGG + chr4.2:23233489-23233508 None:intergenic 40.0%
!! TGTTTTTGCCTTTGGACTTG+CGG + chr4.2:23233380-23233399 None:intergenic 40.0%
!!! TTGGTTTTCAGGTTTTGGTG+AGG - chr4.2:23234263-23234282 MS.gene39544:intron 40.0%
AAACCATTGAAGAGAGGGGA+GGG + chr4.2:23232681-23232700 None:intergenic 45.0%
AACAGGTGCACTCATGTCAA+TGG + chr4.2:23234036-23234055 None:intergenic 45.0%
CCACGTAATAATCAGCTCCA+TGG + chr4.2:23234455-23234474 None:intergenic 45.0%
CCATGGAGCTGATTATTACG+TGG - chr4.2:23234452-23234471 MS.gene39544:CDS 45.0%
CTGGAAGCAATCTACAACTC+AGG - chr4.2:23233255-23233274 MS.gene39544:CDS 45.0%
GAAGTAATCGAGGTTAACGC+AGG - chr4.2:23234324-23234343 MS.gene39544:intron 45.0%
TACTGCCAACGAAGTAATCG+AGG - chr4.2:23234314-23234333 MS.gene39544:intron 45.0%
TGGTTGGAGTTCGACGAATT+TGG + chr4.2:23232637-23232656 None:intergenic 45.0%
TTAGGAGCTGGATCTGCAAT+TGG + chr4.2:23233438-23233457 None:intergenic 45.0%
TTTCATTGGGAGAGGCTCTT+TGG + chr4.2:23233524-23233543 None:intergenic 45.0%
! AGAGGCTCTTTGGTGATTAG+AGG + chr4.2:23233514-23233533 None:intergenic 45.0%
! GTGATGCGAGTGTCTTTTAC+TGG - chr4.2:23233346-23233365 MS.gene39544:CDS 45.0%
! TCCATGGTAGAGTGAGTTGA+GGG + chr4.2:23234439-23234458 None:intergenic 45.0%
!! GATGAAGATGGATTAGGTGG+TGG + chr4.2:23233486-23233505 None:intergenic 45.0%
!! TCAGGTTTTGGTGAGGAAGA+TGG - chr4.2:23234270-23234289 MS.gene39544:intron 45.0%
!!! GTTTTGGTTCGGAGAGATTG+TGG + chr4.2:23233613-23233632 None:intergenic 45.0%
ACCCTCAACTCACTCTACCA+TGG - chr4.2:23234435-23234454 MS.gene39544:CDS 50.0%
ATGCACATTGCACACGTCCA+TGG + chr4.2:23234174-23234193 None:intergenic 50.0%
ATGGACGTGTGCAATGTGCA+TGG - chr4.2:23234173-23234192 MS.gene39544:intron 50.0%
CTCCATGGTAGAGTGAGTTG+AGG + chr4.2:23234440-23234459 None:intergenic 50.0%
GAAACCATTGAAGAGAGGGG+AGG + chr4.2:23232682-23232701 None:intergenic 50.0%
GGGAGAGAGTTATTAGGAGC+TGG + chr4.2:23233450-23233469 None:intergenic 50.0%
GTTTGACTCCTTCTGTACCC+CGG - chr4.2:23234389-23234408 MS.gene39544:intron 50.0%
TAACCCTCCCCTCTCTTCAA+TGG - chr4.2:23232675-23232694 MS.gene39544:CDS 50.0%
TGATCTTGCTCCTCTACCTC+CGG - chr4.2:23234010-23234029 MS.gene39544:intron 50.0%
TGCACATTGCACACGTCCAT+GGG + chr4.2:23234173-23234192 None:intergenic 50.0%
! ATGCGGATTTGCTCAGGCTT+TGG + chr4.2:23233234-23233253 None:intergenic 50.0%
! CCATTCATCGGTTAGAACCG+GGG + chr4.2:23234409-23234428 None:intergenic 50.0%
! CCCATTCATCGGTTAGAACC+GGG + chr4.2:23234410-23234429 None:intergenic 50.0%
! CCCCATTCATCGGTTAGAAC+CGG + chr4.2:23234411-23234430 None:intergenic 50.0%
! CCCGGTTCTAACCGATGAAT+GGG - chr4.2:23234407-23234426 MS.gene39544:intron 50.0%
! CCGGTTCTAACCGATGAATG+GGG - chr4.2:23234408-23234427 MS.gene39544:intron 50.0%
! TCCAGAATGCGGATTTGCTC+AGG + chr4.2:23233240-23233259 None:intergenic 50.0%
! TGCGGATTTGCTCAGGCTTT+GGG + chr4.2:23233233-23233252 None:intergenic 50.0%
! TTTGGGTTCCATCTTGGCCT+TGG + chr4.2:23233216-23233235 None:intergenic 50.0%
!! ATGGATTAGGTGGTGGTGGT+GGG + chr4.2:23233479-23233498 None:intergenic 50.0%
!! GAAGATGGATTAGGTGGTGG+TGG + chr4.2:23233483-23233502 None:intergenic 50.0%
!! TCAGGCTTTGGGTTCCATCT+TGG + chr4.2:23233222-23233241 None:intergenic 50.0%
ATCCTGGACCAAGGCCAAGA+TGG - chr4.2:23233205-23233224 MS.gene39544:CDS 55.0%
ATGTCAATGGCCGGAGGTAG+AGG + chr4.2:23234023-23234042 None:intergenic 55.0%
GAGGGTGACACCCCATTCAT+CGG + chr4.2:23234421-23234440 None:intergenic 55.0%
GCCTGAGCAAATCCGCATTC+TGG - chr4.2:23233236-23233255 MS.gene39544:CDS 55.0%
GGTGCACTCATGTCAATGGC+CGG + chr4.2:23234032-23234051 None:intergenic 55.0%
GGTTAGAACCGGGGTACAGA+AGG + chr4.2:23234400-23234419 None:intergenic 55.0%
! CCCCGGTTCTAACCGATGAA+TGG - chr4.2:23234406-23234425 MS.gene39544:intron 55.0%
!! GATGGATTAGGTGGTGGTGG+TGG + chr4.2:23233480-23233499 None:intergenic 55.0%
!! GTGGTGGTGGTGGGATTTGT+GGG + chr4.2:23233470-23233489 None:intergenic 55.0%
!! TTCCATCTTGGCCTTGGTCC+AGG + chr4.2:23233210-23233229 None:intergenic 55.0%
GCACTCATGTCAATGGCCGG+AGG + chr4.2:23234029-23234048 None:intergenic 60.0%
!! GGTGGTGGTGGTGGGATTTG+TGG + chr4.2:23233471-23233490 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23232586 23234515 23232586 ID=MS.gene39544
chr4.2 mRNA 23232586 23234515 23232586 ID=MS.gene39544.t1;Parent=MS.gene39544
chr4.2 exon 23234428 23234515 23234428 ID=MS.gene39544.t1.exon1;Parent=MS.gene39544.t1
chr4.2 CDS 23234428 23234515 23234428 ID=cds.MS.gene39544.t1;Parent=MS.gene39544.t1
chr4.2 exon 23233009 23233917 23233009 ID=MS.gene39544.t1.exon2;Parent=MS.gene39544.t1
chr4.2 CDS 23233009 23233917 23233009 ID=cds.MS.gene39544.t1;Parent=MS.gene39544.t1
chr4.2 exon 23232586 23232827 23232586 ID=MS.gene39544.t1.exon3;Parent=MS.gene39544.t1
chr4.2 CDS 23232586 23232827 23232586 ID=cds.MS.gene39544.t1;Parent=MS.gene39544.t1
Gene Sequence

>MS.gene39544

ATGGCTCGTAAGAAGAAAGTACATTGGCCAAGCAAGTTTATAAAGTCACCAAATTCGTCGAACTCCAACCATCAAAATCGTCTGCATGGTAACCCTCCCCTCTCTTCAATGGTTTCCAAACAACTCCTTCTACTTTTTTTTTATTATTATTATTATTTAATCAAACTTACATTTATGTTTGTATGAAAGATCAAAATCAATTACTCTACAAACTCAAAATTCCAATCGAAACAAATTATATCGATTTAGTTTTTTTTCCATGTTAGGGGACATATAAACAAATCAAACCAAACAAACATGATCTCTACTATTTGATCTTTCCTAATCTTAGCTTTAAAAAAATTAAAAAGCCTAAATCTAAAATCAACTATTTGATCTTTTCTAATGGTCTGACCATATTTGCTTGACGGTGTAAATTTAGGATTTCAATGCAAGTAAGGATTCCTAAATTCACACATTCAAGCAAATATAACCCATAAAAAATAGTTTTAGAAATATCATAATACTATGCTTCATATAGTGTAATGCATATATTTTGATCAATTTTATAATTTGTAAACTTTGCATGTTTGATCGGCTTATAACAAATATTGTTTAGGTGGAGATAATGAAGAAAGAAATCCTGGACCAAGGCCAAGATGGAACCCAAAGCCTGAGCAAATCCGCATTCTGGAAGCAATCTACAACTCAGGGATGAAGAATCCACCTGTAGATGAAATCAAGAAGATACGTGAACAATTAGAAGAATTTGGGAAAATTGGTGATGCGAGTGTCTTTTACTGGTTTCAAAACCGCAAGTCCAAAGGCAAAAACAAAAAAGCACCCTACCCCAAGAAATCAAAAAGACAACCAATTGCAGATCCAGCTCCTAATAACTCTCTCCCACAAATCCCACCACCACCACCTAATCCATCTTCATCATCTTCCTCTAATCACCAAAGAGCCTCTCCCAATGAAATCACAATATCTAACAATATTGGAATCTCCAATATTAATGATGGGAGAGTGTTGGTGCCTAATTCATCCACAATCTCTCCGAACCAAAACCAAGATGATACTTATTCTCACACCCCAACTGAAACCGATCTACAACTACCTAAAACCCTATTTTCCTCTTTCTCAATGGAGAATCATGTTAATGAAGGGGTAGTGCTTAATGACATGACAACACTAGAGGGGTTTAATTACTTCTCTGATTTCCCGAATATGATTGAACCCTCACCTCAACAAAATGTTGATCTTCCTGTGCTTAATTACGATAACATCATGAACAATGAAAATGAAAATGGAATCTGCTCAACCAATGATCTTTTTGATCAAGTGAATGTTCAAGAGGAAGCCATGAATATGATGCACATGCACCAACAAGACCCGCAATTTAATTTTGGTGTCACAACAGCATCAAGTAATGATGATCATTCAACTGATCTTGCTCCTCTACCTCCGGCCATTGACATGAGTGCACCTGTTGATATTCCATTCCTTTTTACCGATGATCAACTTCAAGGTAATCAATATCAATTTTTACAATATATATATACACCCAAAATTTCAAGCACAAGGGTATGATTATTTTTTAATTATCCCATGGACGTGTGCAATGTGCATGGCTTAATGTTTTTTTTTTTTTTTTTTTTTTTTGTGAACAACATGTATATTATAACGATTAGTTGATGTTTGGTTTTCAGGTTTTGGTGAGGAAGATGGTCTGAGAAAATGTAGAATTTTTACTGCCAACGAAGTAATCGAGGTTAACGCAGGGCCCTTTAACGTTCGTGAATCCTTTGGTAATAGAGCAGTTCTGTTTGACTCCTTCTGTACCCCGGTTCTAACCGATGAATGGGGTGTCACCCTCAACTCACTCTACCATGGAGCTGATTATTACGTGGTAAAATCAACTTTTTCTTATCTACTACGCTTTGTTCATTAA
Protein sequence

>MS.gene39544.t1

MARKKKVHWPSKFIKSPNSSNSNHQNRLHGGDNEERNPGPRPRWNPKPEQIRILEAIYNSGMKNPPVDEIKKIREQLEEFGKIGDASVFYWFQNRKSKGKNKKAPYPKKSKRQPIADPAPNNSLPQIPPPPPNPSSSSSSNHQRASPNEITISNNIGISNINDGRVLVPNSSTISPNQNQDDTYSHTPTETDLQLPKTLFSSFSMENHVNEGVVLNDMTTLEGFNYFSDFPNMIEPSPQQNVDLPVLNYDNIMNNENENGICSTNDLFDQVNVQEEAMNMMHMHQQDPQFNFGVTTASSNDDHSTDLAPLPPAIDMSAPVDIPFLFTDDQLQGFGEEDGLRKCRIFTANEVIEVNAGPFNVRESFGNRAVLFDSFCTPVLTDEWGVTLNSLYHGADYYVVKSTFSYLLRFVH