Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39769.t1 | XP_003618052.1 | 74.2 | 248 | 60 | 2 | 1 | 248 | 27 | 270 | 2.30E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39769.t1 | G7K0M6 | 74.2 | 248 | 60 | 2 | 1 | 248 | 27 | 270 | 1.7e-78 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene39769.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39769.t1 | MTR_5g098470 | 74.597 | 248 | 59 | 2 | 1 | 248 | 27 | 270 | 3.92e-128 | 365 |
| MS.gene39769.t1 | MTR_1g046810 | 28.763 | 299 | 137 | 14 | 36 | 288 | 37 | 305 | 9.42e-14 | 70.5 |
| MS.gene39769.t1 | MTR_6g081160 | 28.481 | 158 | 95 | 4 | 40 | 187 | 30 | 179 | 3.81e-12 | 65.5 |
| MS.gene39769.t1 | MTR_1g052185 | 30.476 | 210 | 112 | 6 | 19 | 218 | 9 | 194 | 5.20e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39769.t1 | AT5G56200 | 46.429 | 84 | 41 | 1 | 38 | 117 | 77 | 160 | 6.18e-14 | 72.0 |
Find 45 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGACGATGATTCAAAATTT+TGG | 0.153043 | 5.4:+69684372 | MS.gene39769:CDS |
| TCAACATGATGATGACTATT+TGG | 0.187915 | 5.4:-69684005 | None:intergenic |
| ATACCCCTCCATTCCCTTTC+AGG | 0.219356 | 5.4:-69684260 | None:intergenic |
| GTGACCATATAAGGCCTTAT+TGG | 0.260984 | 5.4:-69684225 | None:intergenic |
| CTTCTTCTCTATGGATTTCA+TGG | 0.270549 | 5.4:-69683931 | None:intergenic |
| TTTAGGTGTGGAAAAGCATT+AGG | 0.279524 | 5.4:+69684077 | MS.gene39769:CDS |
| CCATCCAATAAGGCCTTATA+TGG | 0.323281 | 5.4:+69684221 | MS.gene39769:CDS |
| AGATCTCATGTGACCATATA+AGG | 0.346698 | 5.4:-69684234 | None:intergenic |
| TGCAATTCTTCTAATGATAG+TGG | 0.351010 | 5.4:+69684164 | MS.gene39769:CDS |
| CACTCGTTCAAATCAAAATC+TGG | 0.351225 | 5.4:-69684776 | None:intergenic |
| GATCCCATCTTCAAGCTCTA+AGG | 0.358194 | 5.4:+69684111 | MS.gene39769:CDS |
| AAAGAATTTCCCATCCAATA+AGG | 0.359792 | 5.4:+69684211 | MS.gene39769:CDS |
| GAGGGGTATGTATCCTAATA+AGG | 0.367738 | 5.4:+69684274 | MS.gene39769:CDS |
| TTAGGTGTGGAAAAGCATTA+GGG | 0.371642 | 5.4:+69684078 | MS.gene39769:CDS |
| CCTAATAAGGATAAAACTAT+TGG | 0.385116 | 5.4:+69684287 | MS.gene39769:CDS |
| CATCATGTTGAAGGATCAAC+AGG | 0.420630 | 5.4:+69684017 | MS.gene39769:CDS |
| CTTTCCTTAGAGCTTGAAGA+TGG | 0.421663 | 5.4:-69684115 | None:intergenic |
| CCTGGTACAAGTGGTCTTGT+TGG | 0.433511 | 5.4:+69684548 | MS.gene39769:CDS |
| GCAAAGAGATGAATTGTAAT+GGG | 0.433880 | 5.4:+69684708 | MS.gene39769:CDS |
| AATAGTCATCATCATGTTGA+AGG | 0.464152 | 5.4:+69684008 | MS.gene39769:CDS |
| TACCCCTCCATTCCCTTTCA+GGG | 0.465209 | 5.4:-69684259 | None:intergenic |
| CCACCAATATGGTTTAAAAC+CGG | 0.477481 | 5.4:+69684395 | MS.gene39769:CDS |
| CCATATAAGGCCTTATTGGA+TGG | 0.492046 | 5.4:-69684221 | None:intergenic |
| CATGAGATCTCACCCTGAAA+GGG | 0.502988 | 5.4:+69684247 | MS.gene39769:CDS |
| TGCCATCATAAGTTCCACTT+TGG | 0.505420 | 5.4:-69684688 | None:intergenic |
| GGCAAAGAGATGAATTGTAA+TGG | 0.511018 | 5.4:+69684707 | MS.gene39769:CDS |
| TTCGGATGACGAGGATAAGA+AGG | 0.520435 | 5.4:+69684514 | MS.gene39769:CDS |
| ACATGAGATCTCACCCTGAA+AGG | 0.521597 | 5.4:+69684246 | MS.gene39769:CDS |
| TGGTTTAAAACCGGCAAAAG+AGG | 0.523461 | 5.4:+69684404 | MS.gene39769:CDS |
| TGACAACTTCAATGCAGATT+CGG | 0.527965 | 5.4:+69684496 | MS.gene39769:CDS |
| AGAATGACAATAACAAAAGA+AGG | 0.532053 | 5.4:+69684594 | MS.gene39769:CDS |
| TACCAAAGTGGAACTTATGA+TGG | 0.540351 | 5.4:+69684686 | MS.gene39769:CDS |
| CCAACAAGACCACTTGTACC+AGG | 0.547377 | 5.4:-69684548 | None:intergenic |
| TTTCCTTAGAGCTTGAAGAT+GGG | 0.556262 | 5.4:-69684114 | None:intergenic |
| TCTTCAAGCTCTAAGGAAAG+AGG | 0.559146 | 5.4:+69684118 | MS.gene39769:CDS |
| CATATAAGGCCTTATTGGAT+GGG | 0.559243 | 5.4:-69684220 | None:intergenic |
| GATCTCACCCTGAAAGGGAA+TGG | 0.563550 | 5.4:+69684252 | MS.gene39769:CDS |
| TCACCCTGAAAGGGAATGGA+GGG | 0.580204 | 5.4:+69684256 | MS.gene39769:CDS |
| CTCACCCTGAAAGGGAATGG+AGG | 0.583418 | 5.4:+69684255 | MS.gene39769:CDS |
| CTTCAAGCTCTAAGGAAAGA+GGG | 0.588989 | 5.4:+69684119 | MS.gene39769:CDS |
| CAATGCAGATTCGGATGACG+AGG | 0.631053 | 5.4:+69684505 | MS.gene39769:CDS |
| AGGTGTGGAAAAGCATTAGG+GGG | 0.631959 | 5.4:+69684080 | MS.gene39769:CDS |
| TAGGTGTGGAAAAGCATTAG+GGG | 0.641738 | 5.4:+69684079 | MS.gene39769:CDS |
| CACCCTGAAAGGGAATGGAG+GGG | 0.655548 | 5.4:+69684257 | MS.gene39769:CDS |
| AGAGGGTGAGTTGAAAACAA+AGG | 0.686503 | 5.4:+69684136 | MS.gene39769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGTAACAAAATTTTT+AGG | + | chr5.4:69684060-69684079 | MS.gene39769:CDS | 10.0% |
| !! | ATCTAAAAACAAAATCTTGT+TGG | + | chr5.4:69684652-69684671 | MS.gene39769:CDS | 20.0% |
| !!! | TTGTTTTTAGATATTGAACA+TGG | - | chr5.4:69684644-69684663 | None:intergenic | 20.0% |
| ! | AGAATGACAATAACAAAAGA+AGG | + | chr5.4:69684594-69684613 | MS.gene39769:CDS | 25.0% |
| ! | CCTAATAAGGATAAAACTAT+TGG | + | chr5.4:69684287-69684306 | MS.gene39769:CDS | 25.0% |
| !! | CAAATCTTTTGAATTGATTG+CGG | - | chr5.4:69684348-69684367 | None:intergenic | 25.0% |
| !! | TAAACTTTTCTTCTTCTCTA+TGG | - | chr5.4:69683943-69683962 | None:intergenic | 25.0% |
| !!! | CCAATAGTTTTATCCTTATT+AGG | - | chr5.4:69684290-69684309 | None:intergenic | 25.0% |
| !!! | TGTAACAAAATTTTTAGGTG+TGG | + | chr5.4:69684065-69684084 | MS.gene39769:CDS | 25.0% |
| AAAGAATTTCCCATCCAATA+AGG | + | chr5.4:69684211-69684230 | MS.gene39769:CDS | 30.0% | |
| AATAGTCATCATCATGTTGA+AGG | + | chr5.4:69684008-69684027 | MS.gene39769:CDS | 30.0% | |
| GCAAAGAGATGAATTGTAAT+GGG | + | chr5.4:69684708-69684727 | MS.gene39769:CDS | 30.0% | |
| TAACAAAAGAAGGAAAAAGC+TGG | + | chr5.4:69684604-69684623 | MS.gene39769:CDS | 30.0% | |
| TGCAATTCTTCTAATGATAG+TGG | + | chr5.4:69684164-69684183 | MS.gene39769:CDS | 30.0% | |
| ! | AAGAAGGAGAATATTTTACC+TGG | + | chr5.4:69684530-69684549 | MS.gene39769:CDS | 30.0% |
| ! | AATATTTTACCTGGTACAAG+TGG | + | chr5.4:69684539-69684558 | MS.gene39769:CDS | 30.0% |
| ! | TCAACATGATGATGACTATT+TGG | - | chr5.4:69684008-69684027 | None:intergenic | 30.0% |
| ! | TCGACGATGATTCAAAATTT+TGG | + | chr5.4:69684372-69684391 | MS.gene39769:CDS | 30.0% |
| !!! | GTAATGGGTATTTTGAGATT+GGG | + | chr5.4:69684723-69684742 | MS.gene39769:CDS | 30.0% |
| !!! | TAATGGGTATTTTGAGATTG+GGG | + | chr5.4:69684724-69684743 | MS.gene39769:CDS | 30.0% |
| !!! | TGTAATGGGTATTTTGAGAT+TGG | + | chr5.4:69684722-69684741 | MS.gene39769:CDS | 30.0% |
| AGATCTCATGTGACCATATA+AGG | - | chr5.4:69684237-69684256 | None:intergenic | 35.0% | |
| CACTCGTTCAAATCAAAATC+TGG | - | chr5.4:69684779-69684798 | None:intergenic | 35.0% | |
| GAAAAAGATTGCTACCAAAG+TGG | + | chr5.4:69684674-69684693 | MS.gene39769:CDS | 35.0% | |
| GGCAAAGAGATGAATTGTAA+TGG | + | chr5.4:69684707-69684726 | MS.gene39769:CDS | 35.0% | |
| TACCAAAGTGGAACTTATGA+TGG | + | chr5.4:69684686-69684705 | MS.gene39769:CDS | 35.0% | |
| TGACAACTTCAATGCAGATT+CGG | + | chr5.4:69684496-69684515 | MS.gene39769:CDS | 35.0% | |
| TTTCCTTAGAGCTTGAAGAT+GGG | - | chr5.4:69684117-69684136 | None:intergenic | 35.0% | |
| ! | CCACCAATATGGTTTAAAAC+CGG | + | chr5.4:69684395-69684414 | MS.gene39769:CDS | 35.0% |
| !! | CAAAATTTTGGCCACCAATA+TGG | + | chr5.4:69684384-69684403 | MS.gene39769:CDS | 35.0% |
| !! | CATATAAGGCCTTATTGGAT+GGG | - | chr5.4:69684223-69684242 | None:intergenic | 35.0% |
| !! | TTAGGTGTGGAAAAGCATTA+GGG | + | chr5.4:69684078-69684097 | MS.gene39769:CDS | 35.0% |
| !! | TTTAGGTGTGGAAAAGCATT+AGG | + | chr5.4:69684077-69684096 | MS.gene39769:CDS | 35.0% |
| !!! | TTGCCGGTTTTAAACCATAT+TGG | - | chr5.4:69684401-69684420 | None:intergenic | 35.0% |
| CATCATGTTGAAGGATCAAC+AGG | + | chr5.4:69684017-69684036 | MS.gene39769:CDS | 40.0% | |
| CTTTCCTTAGAGCTTGAAGA+TGG | - | chr5.4:69684118-69684137 | None:intergenic | 40.0% | |
| TGCCATCATAAGTTCCACTT+TGG | - | chr5.4:69684691-69684710 | None:intergenic | 40.0% | |
| ! | AGAGGGTGAGTTGAAAACAA+AGG | + | chr5.4:69684136-69684155 | MS.gene39769:CDS | 40.0% |
| ! | CCATCCAATAAGGCCTTATA+TGG | + | chr5.4:69684221-69684240 | MS.gene39769:CDS | 40.0% |
| ! | GAGGGGTATGTATCCTAATA+AGG | + | chr5.4:69684274-69684293 | MS.gene39769:CDS | 40.0% |
| ! | GTGACCATATAAGGCCTTAT+TGG | - | chr5.4:69684228-69684247 | None:intergenic | 40.0% |
| ! | TGGTTTAAAACCGGCAAAAG+AGG | + | chr5.4:69684404-69684423 | MS.gene39769:CDS | 40.0% |
| !! | CCATATAAGGCCTTATTGGA+TGG | - | chr5.4:69684224-69684243 | None:intergenic | 40.0% |
| !! | CTTCAAGCTCTAAGGAAAGA+GGG | + | chr5.4:69684119-69684138 | MS.gene39769:CDS | 40.0% |
| !! | TAGGTGTGGAAAAGCATTAG+GGG | + | chr5.4:69684079-69684098 | MS.gene39769:CDS | 40.0% |
| !! | TCTTCAAGCTCTAAGGAAAG+AGG | + | chr5.4:69684118-69684137 | MS.gene39769:CDS | 40.0% |
| !!! | CCGGTTTTAAACCATATTGG+TGG | - | chr5.4:69684398-69684417 | None:intergenic | 40.0% |
| ACATGAGATCTCACCCTGAA+AGG | + | chr5.4:69684246-69684265 | MS.gene39769:CDS | 45.0% | |
| CATGAGATCTCACCCTGAAA+GGG | + | chr5.4:69684247-69684266 | MS.gene39769:CDS | 45.0% | |
| GATCCCATCTTCAAGCTCTA+AGG | + | chr5.4:69684111-69684130 | MS.gene39769:CDS | 45.0% | |
| TTCGGATGACGAGGATAAGA+AGG | + | chr5.4:69684514-69684533 | MS.gene39769:CDS | 45.0% | |
| !! | AGGTGTGGAAAAGCATTAGG+GGG | + | chr5.4:69684080-69684099 | MS.gene39769:CDS | 45.0% |
| ATACCCCTCCATTCCCTTTC+AGG | - | chr5.4:69684263-69684282 | None:intergenic | 50.0% | |
| CAATGCAGATTCGGATGACG+AGG | + | chr5.4:69684505-69684524 | MS.gene39769:CDS | 50.0% | |
| CCAACAAGACCACTTGTACC+AGG | - | chr5.4:69684551-69684570 | None:intergenic | 50.0% | |
| GATCTCACCCTGAAAGGGAA+TGG | + | chr5.4:69684252-69684271 | MS.gene39769:CDS | 50.0% | |
| TACCCCTCCATTCCCTTTCA+GGG | - | chr5.4:69684262-69684281 | None:intergenic | 50.0% | |
| TCACCCTGAAAGGGAATGGA+GGG | + | chr5.4:69684256-69684275 | MS.gene39769:CDS | 50.0% | |
| ! | GCTAGAACGTCCTCTTTTGC+CGG | - | chr5.4:69684417-69684436 | None:intergenic | 50.0% |
| !! | CCTGGTACAAGTGGTCTTGT+TGG | + | chr5.4:69684548-69684567 | MS.gene39769:CDS | 50.0% |
| CACCCTGAAAGGGAATGGAG+GGG | + | chr5.4:69684257-69684276 | MS.gene39769:CDS | 55.0% | |
| CTCACCCTGAAAGGGAATGG+AGG | + | chr5.4:69684255-69684274 | MS.gene39769:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 69683933 | 69684799 | 69683933 | ID=MS.gene39769 |
| chr5.4 | mRNA | 69683933 | 69684799 | 69683933 | ID=MS.gene39769.t1;Parent=MS.gene39769 |
| chr5.4 | exon | 69683933 | 69684799 | 69683933 | ID=MS.gene39769.t1.exon1;Parent=MS.gene39769.t1 |
| chr5.4 | CDS | 69683933 | 69684799 | 69683933 | ID=cds.MS.gene39769.t1;Parent=MS.gene39769.t1 |
| Gene Sequence |
| Protein sequence |
