AlfalfaGEDB | MS.gene39778

M. sativa cultivar XinJiangDaYe / MS.gene39778

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39778.t1	XP_013442172.1	93.1	72	5	0	21	92	651	722	1.30E-30	142.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39778.t1	O64477	70.8	72	21	0	21	92	652	723	8.7e-23	107.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39778.t1	A0A072TFN9	93.1	72	5	0	21	92	651	722	9.5e-31	142.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39778.t1	MTR_0280s0040	93.056	72	5	0	21	92	651	722	1.72e-41	144
MS.gene39778.t1	MTR_2g089360	81.944	72	13	0	21	92	653	724	3.57e-35	126
MS.gene39778.t1	MTR_2g089440	66.667	72	24	0	21	92	426	497	7.27e-29	108
MS.gene39778.t1	MTR_2g089440	66.667	72	24	0	21	92	658	729	1.47e-28	107
MS.gene39778.t1	MTR_5g088435	93.478	46	2	1	24	68	1	46	2.59e-24	87.0
MS.gene39778.t1	MTR_1g012550	51.515	66	32	0	21	86	659	724	3.62e-20	83.6
MS.gene39778.t1	MTR_2g073250	53.030	66	31	0	21	86	643	708	1.52e-19	82.0
MS.gene39778.t1	MTR_6g043510	56.061	66	29	0	21	86	648	713	3.11e-19	81.3
MS.gene39778.t1	MTR_2g080080	51.515	66	32	0	21	86	639	704	5.66e-19	80.5
MS.gene39778.t1	MTR_3g107070	48.485	66	34	0	21	86	631	696	6.82e-19	80.1
MS.gene39778.t1	MTR_1g099400	52.174	69	33	0	21	89	681	749	2.83e-18	78.2
MS.gene39778.t1	MTR_6g043790	51.515	66	32	0	21	86	653	718	1.96e-17	75.9
MS.gene39778.t1	MTR_2g103810	48.485	66	34	0	21	86	651	716	2.62e-17	75.5
MS.gene39778.t1	MTR_8g030500	56.164	73	22	2	21	83	641	713	1.93e-16	73.2
MS.gene39778.t1	MTR_4g113710	44.444	72	34	1	21	86	266	337	2.82e-15	69.7
MS.gene39778.t1	MTR_2g080100	50.000	62	30	1	26	86	612	673	4.29e-15	69.3
MS.gene39778.t1	MTR_3g072800	54.717	53	24	0	19	71	676	728	7.11e-15	68.6
MS.gene39778.t1	MTR_2g054870	43.056	72	35	1	21	86	268	339	5.51e-14	66.2
MS.gene39778.t1	MTR_4g114270	56.364	55	21	1	14	65	673	727	2.02e-13	64.7
MS.gene39778.t1	MTR_3g102450	58.537	41	17	0	21	61	205	245	7.76e-13	62.8
MS.gene39778.t1	MTR_1g027370	45.714	70	34	2	9	74	451	520	1.67e-12	62.0
MS.gene39778.t1	MTR_1g032930	69.231	39	12	0	25	63	660	698	3.17e-12	61.2
MS.gene39778.t1	MTR_4g114250	50.909	55	24	1	14	65	675	729	3.18e-12	61.2
MS.gene39778.t1	MTR_3g031580	42.623	61	33	1	13	71	652	712	4.05e-12	60.8
MS.gene39778.t1	MTR_3g031500	42.623	61	33	1	13	71	573	633	4.74e-12	60.5
MS.gene39778.t1	MTR_4g094858	50.000	44	22	0	21	64	670	713	5.42e-12	60.5
MS.gene39778.t1	MTR_7g060570	28.889	90	62	1	2	91	482	569	5.52e-12	60.5
MS.gene39778.t1	MTR_3g031610	48.980	49	25	0	13	61	659	707	6.92e-12	60.1
MS.gene39778.t1	MTR_3g102400	56.098	41	18	0	21	61	676	716	6.93e-12	60.1
MS.gene39778.t1	MTR_3g031510	48.980	49	25	0	13	61	214	262	8.58e-12	59.7
MS.gene39778.t1	MTR_5g013070	52.000	50	24	0	22	71	671	720	1.09e-11	59.7
MS.gene39778.t1	MTR_5g013130	52.000	50	24	0	22	71	668	717	1.68e-11	58.9
MS.gene39778.t1	MTR_3g031640	46.939	49	26	0	13	61	662	710	1.76e-11	58.9
MS.gene39778.t1	MTR_5g006160	51.064	47	23	0	22	68	651	697	2.23e-11	58.9
MS.gene39778.t1	MTR_1g026940	38.667	75	44	1	9	81	450	524	2.46e-11	58.5
MS.gene39778.t1	MTR_7g058550	46.939	49	26	0	13	61	659	707	2.71e-11	58.5
MS.gene39778.t1	MTR_7g058530	46.939	49	26	0	13	61	660	708	2.90e-11	58.5
MS.gene39778.t1	MTR_3g031470	44.898	49	27	0	13	61	673	721	3.42e-11	58.2
MS.gene39778.t1	MTR_7g058830	53.659	41	19	0	21	61	671	711	3.59e-11	58.2
MS.gene39778.t1	MTR_1g027500	44.444	63	33	1	9	69	451	513	3.63e-11	58.2
MS.gene39778.t1	MTR_1g028280	44.595	74	34	3	21	89	532	603	3.70e-11	58.2
MS.gene39778.t1	MTR_3g031480	42.857	49	28	0	13	61	668	716	4.16e-11	58.2
MS.gene39778.t1	MTR_1g027420	44.262	61	32	1	9	67	394	454	4.26e-11	57.8
MS.gene39778.t1	MTR_1g028280	44.595	74	34	3	21	89	493	564	4.31e-11	57.8
MS.gene39778.t1	MTR_7g084220	54.348	46	21	0	22	67	946	991	4.47e-11	57.8
MS.gene39778.t1	MTR_7g053050	53.659	41	19	0	21	61	670	710	4.58e-11	57.8
MS.gene39778.t1	MTR_3g031490	48.780	41	21	0	21	61	665	705	6.38e-11	57.4
MS.gene39778.t1	MTR_3g031600	44.898	49	27	0	13	61	662	710	8.15e-11	57.0
MS.gene39778.t1	MTR_7g058860	51.220	41	20	0	21	61	670	710	8.30e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39778.t1	AT2G19130	70.833	72	21	0	21	92	652	723	1.56e-29	110
MS.gene39778.t1	AT4G32300	53.030	66	31	0	21	86	651	716	6.22e-20	83.2
MS.gene39778.t1	AT5G24080	50.685	73	36	0	21	93	562	634	1.02e-18	79.7
MS.gene39778.t1	AT5G24080	50.685	73	36	0	21	93	562	634	1.02e-18	79.7
MS.gene39778.t1	AT5G24080	50.685	73	36	0	21	93	689	761	1.21e-18	79.3
MS.gene39778.t1	AT1G34300	51.515	66	32	0	21	86	642	707	1.58e-17	76.3
MS.gene39778.t1	AT4G00340	48.649	74	30	1	21	86	639	712	5.73e-16	72.0
MS.gene39778.t1	AT4G00340	48.649	74	30	1	21	86	618	691	5.95e-16	71.6
MS.gene39778.t1	AT5G35370	69.767	43	13	0	21	63	672	714	9.63e-16	71.2
MS.gene39778.t1	AT5G20050	58.140	43	18	0	21	63	269	311	1.33e-12	62.4
MS.gene39778.t1	AT4G04540	47.059	51	27	0	24	74	515	565	8.35e-11	57.0
MS.gene39778.t1	AT4G04540	47.059	51	27	0	24	74	486	536	8.99e-11	57.0
MS.gene39778.t1	AT4G04540	47.059	51	27	0	24	74	515	565	9.11e-11	57.0

Find 31 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCTCCAGAGTGGATTTC+AGG	0.230728	5.4:+69562135	MS.gene39778:CDS
TGTTAGATTCTTTCCTACTT+TGG	0.377893	5.4:+69562257	MS.gene39778:CDS
TTGTCGGAAGGGACTTCTGC+CGG	0.433325	5.4:+69562077	MS.gene39778:CDS
CTCAAGGGAGTGAGGGGACC+CGG	0.445757	5.4:-69562096	None:intergenic
GAATCTAACATGGCCATCTT+CGG	0.452209	5.4:-69562244	None:intergenic
GTTGTAAACATCGGCTTTAG+CGG	0.476466	5.4:-69562169	None:intergenic
TGTCGGAAGGGACTTCTGCC+GGG	0.485045	5.4:+69562078	MS.gene39778:CDS
AACTCCGATCCTTCCGAAGA+TGG	0.489207	5.4:+69562231	MS.gene39778:CDS
TGTTCCTCTCAAGGGAGTGA+GGG	0.496906	5.4:-69562103	None:intergenic
TTCAGCCTAGCAAAGCTTGT+CGG	0.499488	5.4:+69562061	MS.gene39778:CDS
TTGTTCCTCTCAAGGGAGTG+AGG	0.514096	5.4:-69562104	None:intergenic
GGCTAATCACATTCCCACCT+TGG	0.517860	5.4:-69562296	None:intergenic
ATAGCCTCTTGTTCCTCTCA+AGG	0.543969	5.4:-69562112	None:intergenic
ACTGAGTTTGCAGCCAAAGT+AGG	0.545150	5.4:-69562270	None:intergenic
GGTCCCCTCACTCCCTTGAG+AGG	0.547524	5.4:+69562099	MS.gene39778:CDS
ATGGCCATCTTCGGAAGGAT+CGG	0.548369	5.4:-69562235	None:intergenic
TAGCCTCTTGTTCCTCTCAA+GGG	0.551333	5.4:-69562111	None:intergenic
CCCTTCCGACAAGCTTTGCT+AGG	0.551605	5.4:-69562066	None:intergenic
GCCTAGCAAAGCTTGTCGGA+AGG	0.557961	5.4:+69562065	MS.gene39778:CDS
GTTCCTCTCAAGGGAGTGAG+GGG	0.567597	5.4:-69562102	None:intergenic
CCTAGCAAAGCTTGTCGGAA+GGG	0.567919	5.4:+69562066	MS.gene39778:CDS
GAAACTCCTGAAATCCACTC+TGG	0.581913	5.4:-69562141	None:intergenic
AAAGCCGATGTTTACAACTA+CGG	0.603994	5.4:+69562174	MS.gene39778:CDS
CTAACATGGCCATCTTCGGA+AGG	0.620869	5.4:-69562240	None:intergenic
CAAACTCAGTACACCAAGGT+GGG	0.634731	5.4:+69562283	MS.gene39778:CDS
CATTCCGTAGTTGTAAACAT+CGG	0.636880	5.4:-69562178	None:intergenic
ACTCCCTTGAGAGGAACAAG+AGG	0.658194	5.4:+69562108	MS.gene39778:CDS
GAGGCTATCTAGCTCCAGAG+TGG	0.676111	5.4:+69562127	MS.gene39778:CDS
AAGTAGGAAAGAATCTAACA+TGG	0.696541	5.4:-69562254	None:intergenic
GCAAACTCAGTACACCAAGG+TGG	0.702721	5.4:+69562282	MS.gene39778:CDS
GCTGCAAACTCAGTACACCA+AGG	0.759095	5.4:+69562279	MS.gene39778:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTAGGAAAGAATCTAACA+TGG	-	chr5.4:69562257-69562276	None:intergenic	30.0%
	TGTTAGATTCTTTCCTACTT+TGG	+	chr5.4:69562257-69562276	MS.gene39778:CDS	30.0%
!	AGATTCTGATTTTTGTCCAA+AGG	+	chr5.4:69562030-69562049	MS.gene39778:intron	30.0%
!	GAATCTATGAGAATGTTTTC+TGG	-	chr5.4:69562016-69562035	None:intergenic	30.0%
	AAAGCCGATGTTTACAACTA+CGG	+	chr5.4:69562174-69562193	MS.gene39778:CDS	35.0%
	CATTCCGTAGTTGTAAACAT+CGG	-	chr5.4:69562181-69562200	None:intergenic	35.0%
	GAATCTAACATGGCCATCTT+CGG	-	chr5.4:69562247-69562266	None:intergenic	40.0%
!!	GTTGTAAACATCGGCTTTAG+CGG	-	chr5.4:69562172-69562191	None:intergenic	40.0%
	ACTGAGTTTGCAGCCAAAGT+AGG	-	chr5.4:69562273-69562292	None:intergenic	45.0%
	ATAGCCTCTTGTTCCTCTCA+AGG	-	chr5.4:69562115-69562134	None:intergenic	45.0%
	CAAACTCAGTACACCAAGGT+GGG	+	chr5.4:69562283-69562302	MS.gene39778:CDS	45.0%
	GAAACTCCTGAAATCCACTC+TGG	-	chr5.4:69562144-69562163	None:intergenic	45.0%
	TAGCCTCTTGTTCCTCTCAA+GGG	-	chr5.4:69562114-69562133	None:intergenic	45.0%
	TTCAGCCTAGCAAAGCTTGT+CGG	+	chr5.4:69562061-69562080	MS.gene39778:CDS	45.0%
!!	AATGTTTTCTGGCCTCACGT+AGG	-	chr5.4:69562005-69562024	None:intergenic	45.0%
	AACTCCGATCCTTCCGAAGA+TGG	+	chr5.4:69562231-69562250	MS.gene39778:CDS	50.0%
	ACTCCCTTGAGAGGAACAAG+AGG	+	chr5.4:69562108-69562127	MS.gene39778:CDS	50.0%
	AGGCTGAAGTCTGCAACCTT+TGG	-	chr5.4:69562049-69562068	None:intergenic	50.0%
	ATGGCCATCTTCGGAAGGAT+CGG	-	chr5.4:69562238-69562257	None:intergenic	50.0%
	CCTAGCAAAGCTTGTCGGAA+GGG	+	chr5.4:69562066-69562085	MS.gene39778:CDS	50.0%
	CTAACATGGCCATCTTCGGA+AGG	-	chr5.4:69562243-69562262	None:intergenic	50.0%
	GCAAACTCAGTACACCAAGG+TGG	+	chr5.4:69562282-69562301	MS.gene39778:CDS	50.0%
	GCTGCAAACTCAGTACACCA+AGG	+	chr5.4:69562279-69562298	MS.gene39778:CDS	50.0%
	GGCTAATCACATTCCCACCT+TGG	-	chr5.4:69562299-69562318	None:intergenic	50.0%
	GTCTGTACCATTCCTACGTG+AGG	+	chr5.4:69561990-69562009	MS.gene39778:intron	50.0%
	TGTTCCTCTCAAGGGAGTGA+GGG	-	chr5.4:69562106-69562125	None:intergenic	50.0%
	TTGTTCCTCTCAAGGGAGTG+AGG	-	chr5.4:69562107-69562126	None:intergenic	50.0%
!	CTAGCTCCAGAGTGGATTTC+AGG	+	chr5.4:69562135-69562154	MS.gene39778:CDS	50.0%
!	TTTCTGGCCTCACGTAGGAA+TGG	-	chr5.4:69562000-69562019	None:intergenic	50.0%
!!	ATGCTTTTCGAGCTCGTGTC+AGG	+	chr5.4:69562201-69562220	MS.gene39778:CDS	50.0%
	CCCTTCCGACAAGCTTTGCT+AGG	-	chr5.4:69562069-69562088	None:intergenic	55.0%
	GCCTAGCAAAGCTTGTCGGA+AGG	+	chr5.4:69562065-69562084	MS.gene39778:CDS	55.0%
	GTTCCTCTCAAGGGAGTGAG+GGG	-	chr5.4:69562105-69562124	None:intergenic	55.0%
	TTGTCGGAAGGGACTTCTGC+CGG	+	chr5.4:69562077-69562096	MS.gene39778:CDS	55.0%
!	GAGGCTATCTAGCTCCAGAG+TGG	+	chr5.4:69562127-69562146	MS.gene39778:CDS	55.0%
	TGTCGGAAGGGACTTCTGCC+GGG	+	chr5.4:69562078-69562097	MS.gene39778:CDS	60.0%
	CTCAAGGGAGTGAGGGGACC+CGG	-	chr5.4:69562099-69562118	None:intergenic	65.0%
	GGTCCCCTCACTCCCTTGAG+AGG	+	chr5.4:69562099-69562118	MS.gene39778:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	69561976	69562335	69561976	ID=MS.gene39778
chr5.4	mRNA	69561976	69562335	69561976	ID=MS.gene39778.t1;Parent=MS.gene39778
chr5.4	exon	69561976	69561989	69561976	ID=MS.gene39778.t1.exon1;Parent=MS.gene39778.t1
chr5.4	CDS	69561976	69561989	69561976	ID=cds.MS.gene39778.t1;Parent=MS.gene39778.t1
chr5.4	exon	69562059	69562335	69562059	ID=MS.gene39778.t1.exon2;Parent=MS.gene39778.t1
chr5.4	CDS	69562059	69562335	69562059	ID=cds.MS.gene39778.t1;Parent=MS.gene39778.t1

Gene Sequence	>MS.gene39778 ATGAAAAGTGTAGAGTCTGTACCATTCCTACGTGAGGCCAGAAAACATTCTCATAGATTCTGATTTTTGTCCAAAGGTTGCAGACTTCAGCCTAGCAAAGCTTGTCGGAAGGGACTTCTGCCGGGTCCCCTCACTCCCTTGAGAGGAACAAGAGGCTATCTAGCTCCAGAGTGGATTTCAGGAGTTTCTATAACCGCTAAAGCCGATGTTTACAACTACGGAATGATGCTTTTCGAGCTCGTGTCAGGAAGAAGAAACTCCGATCCTTCCGAAGATGGCCATGTTAGATTCTTTCCTACTTTGGCTGCAAACTCAGTACACCAAGGTGGGAATGTGATTAGCCTTAATAATGTCACATGA
Protein sequence	>MS.gene39778.t1 MKSVELQPSKACRKGLLPGPLTPLRGTRGYLAPEWISGVSITAKADVYNYGMMLFELVSGRRNSDPSEDGHVRFFPTLAANSVHQGGNVISLNNVT