AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39940.t1 MTR_1g071360 91.520 342 26 3 1 341 4 343 0.0 622
MS.gene39940.t1 MTR_1g071360 90.801 337 28 3 1 336 1 335 0.0 609
MS.gene39940.t1 MTR_7g095680 54.179 347 113 9 1 315 4 336 7.67e-106 314
MS.gene39940.t1 MTR_1g102860 59.276 221 76 5 17 225 27 245 6.26e-80 247
MS.gene39940.t1 MTR_3g104550 45.000 300 145 5 17 300 33 328 8.74e-76 238
MS.gene39940.t1 MTR_4g077970 50.455 220 90 3 22 224 4 221 8.04e-68 221
MS.gene39940.t1 MTR_5g029680 51.376 218 83 4 28 225 30 244 6.71e-67 219
MS.gene39940.t1 MTR_1g061170 48.848 217 99 3 17 224 36 249 2.68e-60 200
MS.gene39940.t1 MTR_2g100680 42.786 201 105 2 28 221 29 226 1.13e-52 180
MS.gene39940.t1 MTR_6g043060 44.221 199 100 3 30 221 22 216 3.27e-49 168
MS.gene39940.t1 MTR_8g083100 44.221 199 98 4 31 221 23 216 1.28e-47 167
MS.gene39940.t1 MTR_8g105780 47.090 189 82 3 30 200 22 210 1.67e-46 162
MS.gene39940.t1 MTR_8g087540 36.965 257 132 6 30 260 12 264 1.76e-45 160
MS.gene39940.t1 MTR_2g100670 42.308 182 98 1 28 202 31 212 2.31e-45 157
MS.gene39940.t1 MTR_2g100670 42.105 190 99 2 20 202 135 320 2.38e-44 157
MS.gene39940.t1 MTR_6g086805 38.667 225 122 3 30 239 11 234 2.06e-43 153
MS.gene39940.t1 MTR_5g010680 65.957 94 32 0 28 121 22 115 5.73e-42 149
MS.gene39940.t1 MTR_5g017470 41.530 183 63 2 28 166 7 189 3.84e-41 145
MS.gene39940.t1 MTR_0020s0020 63.830 94 34 0 28 121 31 124 1.24e-40 145
MS.gene39940.t1 MTR_1g100777 63.830 94 34 0 28 121 16 109 9.23e-40 142
MS.gene39940.t1 MTR_3g101870 64.078 103 36 1 28 130 22 123 1.76e-39 143
MS.gene39940.t1 MTR_5g089170 43.860 171 65 3 28 168 23 192 9.56e-39 138
MS.gene39940.t1 MTR_7g091370 45.679 162 64 3 21 158 9 170 3.69e-38 136
MS.gene39940.t1 MTR_2g017890 39.560 182 90 4 30 194 15 193 5.90e-37 137
MS.gene39940.t1 MTR_4g088900 38.764 178 90 4 28 193 31 201 5.08e-33 122
MS.gene39940.t1 MTR_1g061170 40.136 147 76 3 87 224 11 154 1.99e-26 108
MS.gene39940.t1 MTR_5g047190 42.857 91 45 2 38 121 1 91 1.45e-16 74.7
MS.gene39940.t1 MTR_4g045757 53.030 66 30 1 30 94 14 79 4.27e-15 70.1
MS.gene39940.t1 MTR_6g044340 30.851 188 111 6 90 267 22 200 2.18e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39940.t1 AT3G22830 49.254 335 125 8 10 300 41 374 2.25e-94 286
MS.gene39940.t1 AT3G51910 52.281 285 90 7 21 300 20 263 6.08e-86 260
MS.gene39940.t1 AT3G63350 44.343 327 115 9 15 331 13 282 2.37e-78 241
MS.gene39940.t1 AT2G26150 49.770 217 98 1 17 222 31 247 1.22e-71 226
MS.gene39940.t1 AT2G26150 49.770 217 98 1 17 222 31 247 1.22e-71 226
MS.gene39940.t1 AT1G32330 50.971 206 81 1 15 200 22 227 5.81e-65 213
MS.gene39940.t1 AT1G32330 50.971 206 81 1 15 200 22 227 6.16e-65 213
MS.gene39940.t1 AT5G43840 42.606 284 141 7 17 289 6 278 2.94e-64 205
MS.gene39940.t1 AT4G17750 44.961 258 109 4 12 239 31 285 3.01e-64 211
MS.gene39940.t1 AT5G16820 48.246 228 92 4 11 221 16 234 1.65e-63 209
MS.gene39940.t1 AT5G16820 48.246 228 92 4 11 221 16 234 1.65e-63 209
MS.gene39940.t1 AT3G02990 48.325 209 88 3 30 221 23 228 1.57e-60 201
MS.gene39940.t1 AT5G03720 47.805 205 87 3 30 220 55 253 1.79e-56 189
MS.gene39940.t1 AT5G03720 47.805 205 87 3 30 220 55 253 1.79e-56 189
MS.gene39940.t1 AT1G67970 45.283 212 101 3 30 229 19 227 2.50e-53 180
MS.gene39940.t1 AT5G54070 41.206 199 111 3 30 223 71 268 2.78e-50 171
MS.gene39940.t1 AT4G13980 43.000 200 104 2 31 223 24 220 2.86e-49 171
MS.gene39940.t1 AT3G24520 41.451 193 101 2 30 222 17 197 1.12e-44 156
MS.gene39940.t1 AT4G36990 60.000 105 42 0 28 132 12 116 8.66e-41 144
MS.gene39940.t1 AT1G46264 37.778 225 81 3 21 187 25 248 1.18e-39 143
MS.gene39940.t1 AT5G62020 62.766 94 35 0 28 121 21 114 1.85e-38 139
MS.gene39940.t1 AT4G18880 43.850 187 93 2 20 194 5 191 4.07e-38 140
MS.gene39940.t1 AT4G11660 62.626 99 36 1 28 126 57 154 8.13e-38 139
MS.gene39940.t1 AT5G45710 35.981 214 118 3 30 228 13 222 1.38e-36 135
MS.gene39940.t1 AT5G45710 35.981 214 118 3 30 228 13 222 1.38e-36 135
MS.gene39940.t1 AT2G41690 62.766 94 35 0 28 121 38 131 6.96e-36 130
MS.gene39940.t1 AT2G26150 43.357 143 70 1 90 221 49 191 3.95e-32 122
MS.gene39940.t1 AT2G26150 43.357 143 70 1 90 221 49 191 3.95e-32 122
MS.gene39940.t1 AT4G18870 52.809 89 37 2 31 117 14 99 1.73e-23 98.6
MS.gene39940.t1 AT4G18870 46.591 88 47 0 30 117 146 233 2.08e-21 92.8
MS.gene39940.t1 AT1G77570 35.227 88 54 2 31 115 27 114 1.24e-11 62.4

Find 68 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTACGTGATTATGAATT+TGG 0.128353 1.4:-58395273 MS.gene39940:CDS
ATGCAGAAATTGATCGTTTA+AGG 0.140595 1.4:-58395593 MS.gene39940:CDS
ATTATTGTGTTTGAAGTATC+TGG 0.159629 1.4:+58395960 None:intergenic
TTCTGTTCCATGAAATTATA+TGG 0.235121 1.4:+58396037 None:intergenic
AACAAGTCCTTCATTTGGTT+GGG 0.266336 1.4:+58396122 None:intergenic
AATTACGTGATTATGAATTT+GGG 0.282722 1.4:-58395272 MS.gene39940:CDS
AACCACTGAGTCAAGAATTT+AGG 0.293001 1.4:-58395020 MS.gene39940:CDS
TTTGCAAATTCCCATCTATC+TGG 0.293795 1.4:+58395711 None:intergenic
CCTTCATTTGGTTGGGGTGG+TGG 0.306285 1.4:+58396129 None:intergenic
ACAACAGACAGAGAAGAAAA+AGG 0.341766 1.4:-58395397 MS.gene39940:CDS
GAGATGGAACATAGGTTAGA+AGG 0.365971 1.4:-58395495 MS.gene39940:CDS
TGCTTGATGATGAAGGGTTC+TGG 0.373815 1.4:-58395131 MS.gene39940:CDS
TCATTTGGTTGGGGTGGTGG+TGG 0.381814 1.4:+58396132 None:intergenic
TCTTCTCTGTCTGTTGTAGT+TGG 0.385092 1.4:+58395403 None:intergenic
ATTTCAACAAGTCCTTCATT+TGG 0.388996 1.4:+58396117 None:intergenic
GTACCAACCATATAATTTCA+TGG 0.396015 1.4:-58396044 MS.gene39940:CDS
TCTTGTGATTCTAGTGCCTC+AGG 0.405629 1.4:+58395168 None:intergenic
TGAATTTGGGGTGTCGGAGT+TGG 0.413715 1.4:-58395259 MS.gene39940:CDS
TGTATCCCAAACCACAAAAC+TGG 0.420129 1.4:+58396008 None:intergenic
CAACAAGTCCTTCATTTGGT+TGG 0.420416 1.4:+58396121 None:intergenic
CCAACACAACCAACTTCAAT+AGG 0.420869 1.4:+58395621 None:intergenic
CTCGGTGAAAGAAGAATACT+TGG 0.431235 1.4:-58396166 MS.gene39940:CDS
GACATCTATTGAGGAACATA+AGG 0.431334 1.4:-58395665 MS.gene39940:CDS
TCATTGTTATCTTCAGAAGT+TGG 0.432296 1.4:+58395063 None:intergenic
CCTATTGAAGTTGGTTGTGT+TGG 0.439872 1.4:-58395621 MS.gene39940:CDS
ATAGATGTCTTTGCCCTCTC+AGG 0.446863 1.4:+58395679 None:intergenic
CCTTCTCAGCCTATTGAAGT+TGG 0.448492 1.4:-58395630 MS.gene39940:CDS
TGGGGTGGTGGTGGAATCGT+TGG 0.458015 1.4:+58396141 None:intergenic
GTATCTGGGAAGGAGATGAT+TGG 0.458621 1.4:+58395975 None:intergenic
ACAAGTCCTTCATTTGGTTG+GGG 0.464149 1.4:+58396123 None:intergenic
GGGGAAAATAATGGATAAAG+AGG 0.470921 1.4:-58395202 MS.gene39940:CDS
AGTCCTTCATTTGGTTGGGG+TGG 0.486652 1.4:+58396126 None:intergenic
GTTCCATGAAATTATATGGT+TGG 0.492988 1.4:+58396041 None:intergenic
ATGTTTAGTATGAAATTTGA+GGG 0.495224 1.4:-58395099 MS.gene39940:CDS
TGATTATGAATTTGGGGTGT+CGG 0.498346 1.4:-58395265 MS.gene39940:CDS
GCTTGATGATGAAGGGTTCT+GGG 0.499647 1.4:-58395130 MS.gene39940:CDS
AATGTTAGCTATGGAAATGC+AGG 0.499978 1.4:-58395235 MS.gene39940:CDS
TTATTGTGTTTGAAGTATCT+GGG 0.502771 1.4:+58395961 None:intergenic
CAAGGAACTAGACTAGGTAT+TGG 0.504481 1.4:-58395327 MS.gene39940:CDS
AATGTTTAGTATGAAATTTG+AGG 0.507525 1.4:-58395100 MS.gene39940:CDS
GAGGAACATAAGGAGAAAGA+AGG 0.510760 1.4:-58395655 MS.gene39940:CDS
GGAGTTGGAAATGTTAGCTA+TGG 0.532242 1.4:-58395244 MS.gene39940:CDS
ACTAGAATCACAAGAGAAGA+TGG 0.536908 1.4:-58395160 MS.gene39940:CDS
CCACCACCCCAACCAAATGA+AGG 0.544676 1.4:-58396129 MS.gene39940:CDS
TGTGTTTGAAGTATCTGGGA+AGG 0.547658 1.4:+58395965 None:intergenic
CAAATGAAAGATTCCTGAGA+GGG 0.550958 1.4:-58395692 MS.gene39940:CDS
ATTACGTGATTATGAATTTG+GGG 0.552478 1.4:-58395271 MS.gene39940:CDS
AGGAACATAAGGAGAAAGAA+GGG 0.559452 1.4:-58395654 MS.gene39940:CDS
GAAAGAGTGCTTGATGATGA+AGG 0.560834 1.4:-58395138 MS.gene39940:CDS
TGTAGTTGGTGAATGAAGAC+AGG 0.561937 1.4:+58395417 None:intergenic
AGATGGAACATAGGTTAGAA+GGG 0.564470 1.4:-58395494 MS.gene39940:CDS
CACACTGTATCTGCTAGAGA+TGG 0.569897 1.4:-58395511 MS.gene39940:CDS
CCAACTTCAATAGGCTGAGA+AGG 0.573783 1.4:+58395630 None:intergenic
ATCCTAAATTCTTGACTCAG+TGG 0.576729 1.4:+58395018 None:intergenic
AAGAGAAAGCTTGTTGAACA+AGG 0.580615 1.4:-58395345 MS.gene39940:CDS
TCATAATCACGTAATTCTAA+CGG 0.586096 1.4:+58395279 None:intergenic
GGGAAGGAGATGATTGGAGA+AGG 0.595385 1.4:+58395981 None:intergenic
GAGGGCAAAGACATCTATTG+AGG 0.598712 1.4:-58395674 MS.gene39940:CDS
ATAATTTCATGGAACAGAAG+TGG 0.614186 1.4:-58396033 MS.gene39940:CDS
GCAAATGAAAGATTCCTGAG+AGG 0.614961 1.4:-58395693 MS.gene39940:CDS
AGAGAAACACGTGTTGATGT+CGG 0.625455 1.4:-58395568 MS.gene39940:CDS
GTTGAACAAGGAACTAGACT+AGG 0.638387 1.4:-58395333 MS.gene39940:CDS
ATCTGCTAGAGATGGAACAT+AGG 0.648151 1.4:-58395503 MS.gene39940:CDS
ACATATATAACATATGTACT+CGG 0.664430 1.4:-58396184 None:intergenic
AGAGGAAGTACATGAACCTG+AGG 0.670115 1.4:-58395184 MS.gene39940:CDS
AAAGAGTGCTTGATGATGAA+GGG 0.670867 1.4:-58395137 MS.gene39940:CDS
GTAGTTGGTGAATGAAGACA+GGG 0.704135 1.4:+58395418 None:intergenic
CATCTCTAGCAGATACAGTG+TGG 0.710812 1.4:+58395512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTTAGTATGAAATTTG+AGG - chr1.4:58396064-58396083 MS.gene39940:CDS 20.0%
!! AATTACGTGATTATGAATTT+GGG - chr1.4:58395892-58395911 MS.gene39940:intron 20.0%
!! ATGTTTAGTATGAAATTTGA+GGG - chr1.4:58396065-58396084 MS.gene39940:CDS 20.0%
! AAAAATTGATCCAGATAGAT+GGG - chr1.4:58395443-58395462 MS.gene39940:CDS 25.0%
! ATTACGTGATTATGAATTTG+GGG - chr1.4:58395893-58395912 MS.gene39940:intron 25.0%
! GAATTACGTGATTATGAATT+TGG - chr1.4:58395891-58395910 MS.gene39940:intron 25.0%
! TAGTAAGTAATTACAGTTCT+CGG - chr1.4:58395358-58395377 MS.gene39940:CDS 25.0%
! TCATAATCACGTAATTCTAA+CGG + chr1.4:58395888-58395907 None:intergenic 25.0%
! TTCTGTTCCATGAAATTATA+TGG + chr1.4:58395130-58395149 None:intergenic 25.0%
!! ATTATTGTGTTTGAAGTATC+TGG + chr1.4:58395207-58395226 None:intergenic 25.0%
!! GTGTTATACAATATTTTGCA+GGG - chr1.4:58395414-58395433 MS.gene39940:CDS 25.0%
!! TGTGTTATACAATATTTTGC+AGG - chr1.4:58395413-58395432 MS.gene39940:CDS 25.0%
!! TTATTGTGTTTGAAGTATCT+GGG + chr1.4:58395206-58395225 None:intergenic 25.0%
ATAATTTCATGGAACAGAAG+TGG - chr1.4:58395131-58395150 MS.gene39940:CDS 30.0%
ATGCAGAAATTGATCGTTTA+AGG - chr1.4:58395571-58395590 MS.gene39940:CDS 30.0%
ATTTCAACAAGTCCTTCATT+TGG + chr1.4:58395050-58395069 None:intergenic 30.0%
GAAAAATTGATCCAGATAGA+TGG - chr1.4:58395442-58395461 MS.gene39940:CDS 30.0%
GTACCAACCATATAATTTCA+TGG - chr1.4:58395120-58395139 MS.gene39940:CDS 30.0%
GTTCCATGAAATTATATGGT+TGG + chr1.4:58395126-58395145 None:intergenic 30.0%
TCATTGTTATCTTCAGAAGT+TGG + chr1.4:58396104-58396123 None:intergenic 30.0%
!! CTACATCAAATGTTTTTGTG+AGG + chr1.4:58395088-58395107 None:intergenic 30.0%
!! TCAAATGTTTTTGTGAGGAA+TGG + chr1.4:58395083-58395102 None:intergenic 30.0%
!!! AAGCTTTCTCTTTTTTGTCA+TGG + chr1.4:58395811-58395830 None:intergenic 30.0%
!!! ATGTTTTCTCGTACTGATTT+TGG - chr1.4:58395383-58395402 MS.gene39940:CDS 30.0%
AACAAGTCCTTCATTTGGTT+GGG + chr1.4:58395045-58395064 None:intergenic 35.0%
AACCACTGAGTCAAGAATTT+AGG - chr1.4:58396144-58396163 MS.gene39940:CDS 35.0%
AATGTTAGCTATGGAAATGC+AGG - chr1.4:58395929-58395948 MS.gene39940:CDS 35.0%
ACAACAGACAGAGAAGAAAA+AGG - chr1.4:58395767-58395786 MS.gene39940:intron 35.0%
ACTAGAATCACAAGAGAAGA+TGG - chr1.4:58396004-58396023 MS.gene39940:CDS 35.0%
AGATGGAACATAGGTTAGAA+GGG - chr1.4:58395670-58395689 MS.gene39940:CDS 35.0%
AGGAACATAAGGAGAAAGAA+GGG - chr1.4:58395510-58395529 MS.gene39940:CDS 35.0%
ATCCTAAATTCTTGACTCAG+TGG + chr1.4:58396149-58396168 None:intergenic 35.0%
CAAATGAAAGATTCCTGAGA+GGG - chr1.4:58395472-58395491 MS.gene39940:CDS 35.0%
GACATCTATTGAGGAACATA+AGG - chr1.4:58395499-58395518 MS.gene39940:CDS 35.0%
TGATCCTAATGTCTCTATCT+AGG - chr1.4:58395311-58395330 MS.gene39940:CDS 35.0%
TTTGCAAATTCCCATCTATC+TGG + chr1.4:58395456-58395475 None:intergenic 35.0%
! TGATTATGAATTTGGGGTGT+CGG - chr1.4:58395899-58395918 MS.gene39940:intron 35.0%
!! AAAGAGTGCTTGATGATGAA+GGG - chr1.4:58396027-58396046 MS.gene39940:CDS 35.0%
!! AAGAGAAAGCTTGTTGAACA+AGG - chr1.4:58395819-58395838 MS.gene39940:intron 35.0%
!! GGGGAAAATAATGGATAAAG+AGG - chr1.4:58395962-58395981 MS.gene39940:CDS 35.0%
!! TTTTGGAAGGGGGAAAATAA+TGG - chr1.4:58395953-58395972 MS.gene39940:CDS 35.0%
ACAAGTCCTTCATTTGGTTG+GGG + chr1.4:58395044-58395063 None:intergenic 40.0%
ATCTGCTAGAGATGGAACAT+AGG - chr1.4:58395661-58395680 MS.gene39940:CDS 40.0%
CAACAAGTCCTTCATTTGGT+TGG + chr1.4:58395046-58395065 None:intergenic 40.0%
CAAGGAACTAGACTAGGTAT+TGG - chr1.4:58395837-58395856 MS.gene39940:intron 40.0%
CCAACACAACCAACTTCAAT+AGG + chr1.4:58395546-58395565 None:intergenic 40.0%
CTCGGTGAAAGAAGAATACT+TGG - chr1.4:58394998-58395017 MS.gene39940:CDS 40.0%
CTGACCTAGATAGAGACATT+AGG + chr1.4:58395318-58395337 None:intergenic 40.0%
GAGATGGAACATAGGTTAGA+AGG - chr1.4:58395669-58395688 MS.gene39940:CDS 40.0%
GAGGAACATAAGGAGAAAGA+AGG - chr1.4:58395509-58395528 MS.gene39940:CDS 40.0%
GCAAATGAAAGATTCCTGAG+AGG - chr1.4:58395471-58395490 MS.gene39940:CDS 40.0%
GGAGTTGGAAATGTTAGCTA+TGG - chr1.4:58395920-58395939 MS.gene39940:intron 40.0%
GTTGAACAAGGAACTAGACT+AGG - chr1.4:58395831-58395850 MS.gene39940:intron 40.0%
TCTTCTCTGTCTGTTGTAGT+TGG + chr1.4:58395764-58395783 None:intergenic 40.0%
TGTATCCCAAACCACAAAAC+TGG + chr1.4:58395159-58395178 None:intergenic 40.0%
! AGAGAAACACGTGTTGATGT+CGG - chr1.4:58395596-58395615 MS.gene39940:CDS 40.0%
! CCTATTGAAGTTGGTTGTGT+TGG - chr1.4:58395543-58395562 MS.gene39940:CDS 40.0%
! GCTATGGAAATGCAGGTTTT+TGG - chr1.4:58395936-58395955 MS.gene39940:CDS 40.0%
! GTTTTTGTGAGGAATGGTTG+TGG + chr1.4:58395077-58395096 None:intergenic 40.0%
! TGTGTTTGAAGTATCTGGGA+AGG + chr1.4:58395202-58395221 None:intergenic 40.0%
!! AAATGCAGGTTTTTGGAAGG+GGG - chr1.4:58395943-58395962 MS.gene39940:CDS 40.0%
!! GAAAGAGTGCTTGATGATGA+AGG - chr1.4:58396026-58396045 MS.gene39940:CDS 40.0%
!! GAAATGCAGGTTTTTGGAAG+GGG - chr1.4:58395942-58395961 MS.gene39940:CDS 40.0%
!! GGAAATGCAGGTTTTTGGAA+GGG - chr1.4:58395941-58395960 MS.gene39940:CDS 40.0%
!! GTAGTTGGTGAATGAAGACA+GGG + chr1.4:58395749-58395768 None:intergenic 40.0%
!! TGGAAATGCAGGTTTTTGGA+AGG - chr1.4:58395940-58395959 MS.gene39940:CDS 40.0%
!! TGTAGTTGGTGAATGAAGAC+AGG + chr1.4:58395750-58395769 None:intergenic 40.0%
!!! TCTCTTTTTTGTCATGGTCG+CGG + chr1.4:58395805-58395824 None:intergenic 40.0%
ATAGATGTCTTTGCCCTCTC+AGG + chr1.4:58395488-58395507 None:intergenic 45.0%
CACACTGTATCTGCTAGAGA+TGG - chr1.4:58395653-58395672 MS.gene39940:CDS 45.0%
CATCTCTAGCAGATACAGTG+TGG + chr1.4:58395655-58395674 None:intergenic 45.0%
CCAACTTCAATAGGCTGAGA+AGG + chr1.4:58395537-58395556 None:intergenic 45.0%
CCTTCTCAGCCTATTGAAGT+TGG - chr1.4:58395534-58395553 MS.gene39940:CDS 45.0%
GAGGGCAAAGACATCTATTG+AGG - chr1.4:58395490-58395509 MS.gene39940:CDS 45.0%
GTATCTGGGAAGGAGATGAT+TGG + chr1.4:58395192-58395211 None:intergenic 45.0%
TCTTGTGATTCTAGTGCCTC+AGG + chr1.4:58395999-58396018 None:intergenic 45.0%
! AGAGGAAGTACATGAACCTG+AGG - chr1.4:58395980-58395999 MS.gene39940:CDS 45.0%
!! GCTTGATGATGAAGGGTTCT+GGG - chr1.4:58396034-58396053 MS.gene39940:CDS 45.0%
!! TGCTTGATGATGAAGGGTTC+TGG - chr1.4:58396033-58396052 MS.gene39940:CDS 45.0%
!! TGGCACCAGTTTTGTGGTTT+GGG - chr1.4:58395151-58395170 MS.gene39940:CDS 45.0%
AGTCCTTCATTTGGTTGGGG+TGG + chr1.4:58395041-58395060 None:intergenic 50.0%
GGGAAGGAGATGATTGGAGA+AGG + chr1.4:58395186-58395205 None:intergenic 50.0%
! CAGAAGTGGCACCAGTTTTG+TGG - chr1.4:58395145-58395164 MS.gene39940:CDS 50.0%
!! GTGGCACCAGTTTTGTGGTT+TGG - chr1.4:58395150-58395169 MS.gene39940:CDS 50.0%
!! TGAATTTGGGGTGTCGGAGT+TGG - chr1.4:58395905-58395924 MS.gene39940:intron 50.0%
CCACCACCCCAACCAAATGA+AGG - chr1.4:58395035-58395054 MS.gene39940:CDS 55.0%
CCTTCATTTGGTTGGGGTGG+TGG + chr1.4:58395038-58395057 None:intergenic 55.0%
! TCATTTGGTTGGGGTGGTGG+TGG + chr1.4:58395035-58395054 None:intergenic 55.0%
!! TGGGGTGGTGGTGGAATCGT+TGG + chr1.4:58395026-58395045 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 58394993 58396193 58394993 ID=MS.gene39940
chr1.4 mRNA 58394993 58396193 58394993 ID=MS.gene39940.t1;Parent=MS.gene39940
chr1.4 exon 58395927 58396193 58395927 ID=MS.gene39940.t1.exon1;Parent=MS.gene39940.t1
chr1.4 CDS 58395927 58396193 58395927 ID=cds.MS.gene39940.t1;Parent=MS.gene39940.t1
chr1.4 exon 58394993 58395751 58394993 ID=MS.gene39940.t1.exon2;Parent=MS.gene39940.t1
chr1.4 CDS 58394993 58395751 58394993 ID=cds.MS.gene39940.t1;Parent=MS.gene39940.t1
Gene Sequence

>MS.gene39940

ATGTACTCGGTGAAAGAAGAATACTTGGAACCAACGATTCCACCACCACCCCAACCAAATGAAGGACTTGTTGAAATTAGACCACAACCATTCCTCACAAAAACATTTGATGTAGTTGATGATCAAAGTACCAACCATATAATTTCATGGAACAGAAGTGGCACCAGTTTTGTGGTTTGGGATACACATGCCTTCTCCAATCATCTCCTTCCCAGATACTTCAAACACAATAATTTCTCAAGTTTTGTCAGACAATTAAACACTTATGTAAGTACTACGTACTCTCTAGATACATACAATTTTTTTTAAATTGAATCATGATCCTAATGTCTCTATCTAGGTCAGATTTTAAAACATTGCTTATATAGTAAGTAATTACAGTTCTCGGCTATGTTTTCTCGTACTGATTTTGGACTTGTGTGTGTTATACAATATTTTGCAGGGATTTAGAAAAATTGATCCAGATAGATGGGAATTTGCAAATGAAAGATTCCTGAGAGGGCAAAGACATCTATTGAGGAACATAAGGAGAAAGAAGGGACCTTCTCAGCCTATTGAAGTTGGTTGTGTTGGACTAGATGCAGAAATTGATCGTTTAAGGCAAGAGAAACACGTGTTGATGTCGGAGCTTGTGAGTCTTAGAAAGCAGCAATATGATACCACACTGTATCTGCTAGAGATGGAACATAGGTTAGAAGGGATAGAAATCAATCAGAAGAAAATGATGTCTTTCTTAGCTAGAGCTATGAAAAACCCTGTCTTCATTCACCAACTACAACAGACAGAGAAGAAAAAGGAGCTTGAAGAATCCGCGACCATGACAAAAAAGAGAAAGCTTGTTGAACAAGGAACTAGACTAGGTATTGGACAATCAAGCAGTGTTAAAGTTGAGCCGTTAGAATTACGTGATTATGAATTTGGGGTGTCGGAGTTGGAAATGTTAGCTATGGAAATGCAGGTTTTTGGAAGGGGGAAAATAATGGATAAAGAGGAAGTACATGAACCTGAGGCACTAGAATCACAAGAGAAGATGGAAAGAGTGCTTGATGATGAAGGGTTCTGGGAAGATCTAATGTTTAGTATGAAATTTGAGGGTAGATTTGACATTCCAACTTCTGAAGATAACAATGAGAATGAAGATGATTCAAGTGAACCACTGAGTCAAGAATTTAGGATTAACTCTTTTCATTCAGTTCACTAG
Protein sequence

>MS.gene39940.t1

MYSVKEEYLEPTIPPPPQPNEGLVEIRPQPFLTKTFDVVDDQSTNHIISWNRSGTSFVVWDTHAFSNHLLPRYFKHNNFSSFVRQLNTYGFRKIDPDRWEFANERFLRGQRHLLRNIRRKKGPSQPIEVGCVGLDAEIDRLRQEKHVLMSELVSLRKQQYDTTLYLLEMEHRLEGIEINQKKMMSFLARAMKNPVFIHQLQQTEKKKELEESATMTKKRKLVEQGTRLGIGQSSSVKVEPLELRDYEFGVSELEMLAMEMQVFGRGKIMDKEEVHEPEALESQEKMERVLDDEGFWEDLMFSMKFEGRFDIPTSEDNNENEDDSSEPLSQEFRINSFHSVH