Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40279.t1 | XP_003613438.2 | 91.2 | 57 | 5 | 0 | 47 | 103 | 28 | 84 | 2.30E-20 | 108.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40279.t1 | O04539 | 80.9 | 47 | 9 | 0 | 56 | 102 | 8 | 54 | 7.2e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40279.t1 | G7K6W4 | 91.2 | 57 | 5 | 0 | 47 | 103 | 28 | 84 | 1.6e-20 | 108.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene40279.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40279.t1 | MTR_5g036670 | 91.228 | 57 | 5 | 0 | 47 | 103 | 28 | 84 | 9.36e-29 | 108 |
| MS.gene40279.t1 | MTR_8g062700 | 61.194 | 67 | 25 | 1 | 38 | 103 | 17 | 83 | 3.08e-20 | 84.3 |
| MS.gene40279.t1 | MTR_8g062700 | 61.194 | 67 | 25 | 1 | 38 | 103 | 17 | 83 | 3.14e-20 | 84.3 |
| MS.gene40279.t1 | MTR_6g008970 | 70.833 | 48 | 14 | 0 | 55 | 102 | 56 | 103 | 4.75e-17 | 75.1 |
| MS.gene40279.t1 | MTR_6g008995 | 63.830 | 47 | 17 | 0 | 56 | 102 | 31 | 77 | 1.30e-13 | 65.5 |
| MS.gene40279.t1 | MTR_1g105955 | 60.417 | 48 | 19 | 0 | 55 | 102 | 9 | 56 | 3.22e-12 | 59.7 |
| MS.gene40279.t1 | MTR_8g062350 | 49.020 | 51 | 26 | 0 | 52 | 102 | 4 | 54 | 6.84e-12 | 59.3 |
| MS.gene40279.t1 | MTR_1g104940 | 59.524 | 42 | 17 | 0 | 62 | 103 | 14 | 55 | 2.15e-11 | 56.2 |
| MS.gene40279.t1 | MTR_1g105960 | 60.000 | 40 | 16 | 0 | 64 | 103 | 3 | 42 | 2.59e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40279.t1 | AT1G70060 | 79.167 | 48 | 10 | 0 | 55 | 102 | 7 | 54 | 1.03e-19 | 82.8 |
| MS.gene40279.t1 | AT1G70060 | 79.167 | 48 | 10 | 0 | 55 | 102 | 7 | 54 | 1.03e-19 | 82.8 |
| MS.gene40279.t1 | AT1G24190 | 72.917 | 48 | 13 | 0 | 55 | 102 | 7 | 54 | 8.54e-18 | 77.4 |
| MS.gene40279.t1 | AT1G24190 | 72.917 | 48 | 13 | 0 | 55 | 102 | 7 | 54 | 9.14e-18 | 77.4 |
| MS.gene40279.t1 | AT1G24190 | 72.917 | 48 | 13 | 0 | 55 | 102 | 7 | 54 | 9.23e-18 | 77.4 |
| MS.gene40279.t1 | AT3G01320 | 68.000 | 50 | 16 | 0 | 53 | 102 | 48 | 97 | 1.35e-17 | 77.0 |
| MS.gene40279.t1 | AT3G01320 | 68.000 | 50 | 16 | 0 | 53 | 102 | 48 | 97 | 1.40e-17 | 76.6 |
| MS.gene40279.t1 | AT1G59890 | 56.522 | 69 | 26 | 1 | 38 | 102 | 17 | 85 | 4.45e-16 | 72.4 |
| MS.gene40279.t1 | AT1G59890 | 56.522 | 69 | 26 | 1 | 38 | 102 | 17 | 85 | 4.54e-16 | 72.4 |
| MS.gene40279.t1 | AT1G59890 | 56.522 | 69 | 26 | 1 | 38 | 102 | 17 | 85 | 4.58e-16 | 72.4 |
| MS.gene40279.t1 | AT1G59890 | 56.522 | 69 | 26 | 1 | 38 | 102 | 17 | 85 | 4.90e-16 | 72.4 |
| MS.gene40279.t1 | AT5G15020 | 65.957 | 47 | 16 | 0 | 56 | 102 | 46 | 92 | 1.89e-15 | 70.9 |
| MS.gene40279.t1 | AT5G15020 | 65.957 | 47 | 16 | 0 | 56 | 102 | 46 | 92 | 1.96e-15 | 70.9 |
| MS.gene40279.t1 | AT5G15025 | 46.154 | 78 | 38 | 2 | 27 | 102 | 12 | 87 | 6.44e-15 | 65.5 |
| MS.gene40279.t1 | AT1G10450 | 70.455 | 44 | 13 | 0 | 60 | 103 | 31 | 74 | 8.55e-15 | 68.9 |
| MS.gene40279.t1 | AT1G10450 | 70.455 | 44 | 13 | 0 | 60 | 103 | 31 | 74 | 8.55e-15 | 68.9 |
| MS.gene40279.t1 | AT1G10450 | 70.455 | 44 | 13 | 0 | 60 | 103 | 82 | 125 | 8.91e-15 | 68.9 |
| MS.gene40279.t1 | AT1G24200 | 52.083 | 48 | 23 | 0 | 55 | 102 | 7 | 54 | 3.96e-12 | 59.7 |
Find 15 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATGTGCCTTCTTATTTGA+AGG | 0.371628 | 3.3:-90964858 | MS.gene40279:intron |
| ATCTCGATTCGGCGGTTGCC+CGG | 0.418989 | 3.3:+90964108 | None:intergenic |
| TAAGATCAAGTTTCAAGGTC+CGG | 0.429714 | 3.3:-90964127 | MS.gene40279:intron |
| TTAATCAAACCTGAGATTGT+TGG | 0.433476 | 3.3:+90964256 | None:intergenic |
| GCTGATGGAGCTGTGGACTT+TGG | 0.439632 | 3.3:-90964886 | None:intergenic |
| TTCTCTAAGATCAAGTTTCA+AGG | 0.445375 | 3.3:-90964132 | MS.gene40279:intron |
| ATTAACGTGCCAACAATCTC+AGG | 0.477289 | 3.3:-90964265 | MS.gene40279:intron |
| AACAATACCTTCAAATAAGA+AGG | 0.490879 | 3.3:+90964851 | None:intergenic |
| AAGATCAAGTTTCAAGGTCC+GGG | 0.505302 | 3.3:-90964126 | MS.gene40279:CDS |
| AGACAAGAAGGAAAAGTATG+AGG | 0.539440 | 3.3:-90964023 | MS.gene40279:CDS |
| AGCATATCTCAAGGCGATCA+AGG | 0.551355 | 3.3:-90964056 | MS.gene40279:CDS |
| TTCAGGGATTGATTCTAATG+TGG | 0.588863 | 3.3:-90964326 | MS.gene40279:intron |
| TGATGTTGTAGCATATCTCA+AGG | 0.606029 | 3.3:-90964065 | MS.gene40279:CDS |
| GGGCAACCGCCGAATCGAGA+TGG | 0.613865 | 3.3:-90964106 | MS.gene40279:CDS |
| ATTTGTAGAGTTGATACTGA+TGG | 0.633224 | 3.3:-90963874 | MS.gene40279:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAAATTTTTGATATTTGA+AGG | - | chr3.3:90964353-90964372 | MS.gene40279:intron | 15.0% |
| !!! | GTTGTTTTTGTATATATATT+GGG | - | chr3.3:90964794-90964813 | MS.gene40279:intron | 15.0% |
| !! | ATATATATACAAAAACAACG+TGG | + | chr3.3:90964793-90964812 | None:intergenic | 20.0% |
| !! | ATATTGCTTTCATGTATTAT+AGG | + | chr3.3:90964189-90964208 | None:intergenic | 20.0% |
| !! | ATGAATTTGAACATAAACTT+TGG | + | chr3.3:90964256-90964275 | None:intergenic | 20.0% |
| !! | TAAATAAGAACAACTACATT+AGG | + | chr3.3:90964321-90964340 | None:intergenic | 20.0% |
| !!! | AATTGATTTGTTTATCCTTT+TGG | - | chr3.3:90964843-90964862 | MS.gene40279:intron | 20.0% |
| !!! | CGTTGTTTTTGTATATATAT+TGG | - | chr3.3:90964793-90964812 | MS.gene40279:intron | 20.0% |
| !!! | GGTAATTTTAAATCTTATAG+CGG | + | chr3.3:90964235-90964254 | None:intergenic | 20.0% |
| !!! | TTAGTGATTTTATTAAAGCT+TGG | - | chr3.3:90964381-90964400 | MS.gene40279:intron | 20.0% |
| ! | AACAATACCTTCAAATAAGA+AGG | + | chr3.3:90963895-90963914 | None:intergenic | 25.0% |
| ! | AAGAGCAAATATTAGCTATA+TGG | - | chr3.3:90963979-90963998 | MS.gene40279:intron | 25.0% |
| ! | TGGACTTTATATATGTTTCA+GGG | - | chr3.3:90964401-90964420 | MS.gene40279:intron | 25.0% |
| ! | TTGGACTTTATATATGTTTC+AGG | - | chr3.3:90964400-90964419 | MS.gene40279:intron | 25.0% |
| ! | TTTGTATATATATTGGGAGA+GGG | - | chr3.3:90964800-90964819 | MS.gene40279:intron | 25.0% |
| !! | TTTTGTATATATATTGGGAG+AGG | - | chr3.3:90964799-90964818 | MS.gene40279:intron | 25.0% |
| !!! | TGAAAGATTTTAAAGCACAA+AGG | - | chr3.3:90964758-90964777 | MS.gene40279:intron | 25.0% |
| ACACAAATCATGTGATTGTT+AGG | - | chr3.3:90964030-90964049 | MS.gene40279:CDS | 30.0% | |
| TCATTTCTTTATTGCTCCTT+CGG | + | chr3.3:90964535-90964554 | None:intergenic | 30.0% | |
| TCTTTGTGTTCACATGTAAA+GGG | - | chr3.3:90963950-90963969 | MS.gene40279:intron | 30.0% | |
| TTAATCAAACCTGAGATTGT+TGG | + | chr3.3:90964490-90964509 | None:intergenic | 30.0% | |
| TTCTTTGTGTTCACATGTAA+AGG | - | chr3.3:90963949-90963968 | MS.gene40279:intron | 30.0% | |
| TTGAGTGTGATCTTATCATA+TGG | + | chr3.3:90964574-90964593 | None:intergenic | 30.0% | |
| TTGTTTACTTAATCTGCATC+AGG | - | chr3.3:90963910-90963929 | MS.gene40279:intron | 30.0% | |
| ! | TCTCAATAGCATTTCAAGTA+AGG | + | chr3.3:90964107-90964126 | None:intergenic | 30.0% |
| !! | ATTTGTAGAGTTGATACTGA+TGG | - | chr3.3:90964869-90964888 | MS.gene40279:CDS | 30.0% |
| !! | TTCTCTAAGATCAAGTTTCA+AGG | - | chr3.3:90964611-90964630 | MS.gene40279:intron | 30.0% |
| !!! | GGATATTTTTCAAGACAAGA+AGG | - | chr3.3:90964708-90964727 | MS.gene40279:intron | 30.0% |
| AACTCTACAAATGCACCAAA+AGG | + | chr3.3:90964861-90964880 | None:intergenic | 35.0% | |
| AGAAACGTGCGTATATCTTA+GGG | - | chr3.3:90964134-90964153 | MS.gene40279:intron | 35.0% | |
| CTTTGTGTTCACATGTAAAG+GGG | - | chr3.3:90963951-90963970 | MS.gene40279:intron | 35.0% | |
| TAAGATCAAGTTTCAAGGTC+CGG | - | chr3.3:90964616-90964635 | MS.gene40279:intron | 35.0% | |
| TAGAAACGTGCGTATATCTT+AGG | - | chr3.3:90964133-90964152 | MS.gene40279:intron | 35.0% | |
| TGATGTTGTAGCATATCTCA+AGG | - | chr3.3:90964678-90964697 | MS.gene40279:intron | 35.0% | |
| TTTGTGTTCACATGTAAAGG+GGG | - | chr3.3:90963952-90963971 | MS.gene40279:intron | 35.0% | |
| ! | AAAGTATGAGGACTTTTTGG+AGG | - | chr3.3:90964732-90964751 | MS.gene40279:intron | 35.0% |
| ! | AGACAAGAAGGAAAAGTATG+AGG | - | chr3.3:90964720-90964739 | MS.gene40279:intron | 35.0% |
| ! | AGTTTTTGACCATCTCGATT+CGG | + | chr3.3:90964649-90964668 | None:intergenic | 35.0% |
| ! | GAATGTGCCTTCTTATTTGA+AGG | - | chr3.3:90963885-90963904 | MS.gene40279:intron | 35.0% |
| ! | TTCAGGGATTGATTCTAATG+TGG | - | chr3.3:90964417-90964436 | MS.gene40279:intron | 35.0% |
| !! | GGAAAAGTATGAGGACTTTT+TGG | - | chr3.3:90964729-90964748 | MS.gene40279:intron | 35.0% |
| AAGATCAAGTTTCAAGGTCC+GGG | - | chr3.3:90964617-90964636 | MS.gene40279:intron | 40.0% | |
| ATTAACGTGCCAACAATCTC+AGG | - | chr3.3:90964478-90964497 | MS.gene40279:intron | 40.0% | |
| CAACTACATTAGGTTCTTCG+CGG | + | chr3.3:90964311-90964330 | None:intergenic | 40.0% | |
| GAAACGTGCGTATATCTTAG+GGG | - | chr3.3:90964135-90964154 | MS.gene40279:intron | 40.0% | |
| GATCACACTCAACATGATTG+TGG | - | chr3.3:90964582-90964601 | MS.gene40279:intron | 40.0% | |
| GTTATTATGCGTGTGTCAAC+AGG | - | chr3.3:90964001-90964020 | MS.gene40279:CDS | 40.0% | |
| TTGTGTTCACATGTAAAGGG+GGG | - | chr3.3:90963953-90963972 | MS.gene40279:intron | 40.0% | |
| AGCATATCTCAAGGCGATCA+AGG | - | chr3.3:90964687-90964706 | MS.gene40279:intron | 45.0% | |
| GTGCGTATATCTTAGGGGAA+AGG | - | chr3.3:90964140-90964159 | MS.gene40279:intron | 45.0% | |
| TTTATTGCTCCTTCGGCTCT+AGG | + | chr3.3:90964528-90964547 | None:intergenic | 45.0% | |
| !!! | TTTTGACCATCTCGATTCGG+CGG | + | chr3.3:90964646-90964665 | None:intergenic | 45.0% |
| TACTTAGTGCCTAGAGCCGA+AGG | - | chr3.3:90964516-90964535 | MS.gene40279:intron | 50.0% | |
| !! | ATCTCGATTCGGCGGTTGCC+CGG | + | chr3.3:90964638-90964657 | None:intergenic | 60.0% |
| GGGCAACCGCCGAATCGAGA+TGG | - | chr3.3:90964637-90964656 | MS.gene40279:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90963861 | 90964904 | 90963861 | ID=MS.gene40279 |
| chr3.3 | mRNA | 90963861 | 90964904 | 90963861 | ID=MS.gene40279.t1;Parent=MS.gene40279 |
| chr3.3 | exon | 90964859 | 90964904 | 90964859 | ID=MS.gene40279.t1.exon1;Parent=MS.gene40279.t1 |
| chr3.3 | CDS | 90964859 | 90964904 | 90964859 | ID=cds.MS.gene40279.t1;Parent=MS.gene40279.t1 |
| chr3.3 | exon | 90964266 | 90964343 | 90964266 | ID=MS.gene40279.t1.exon2;Parent=MS.gene40279.t1 |
| chr3.3 | CDS | 90964266 | 90964343 | 90964266 | ID=cds.MS.gene40279.t1;Parent=MS.gene40279.t1 |
| chr3.3 | exon | 90963986 | 90964145 | 90963986 | ID=MS.gene40279.t1.exon3;Parent=MS.gene40279.t1 |
| chr3.3 | CDS | 90963986 | 90964145 | 90963986 | ID=cds.MS.gene40279.t1;Parent=MS.gene40279.t1 |
| chr3.3 | exon | 90963861 | 90963888 | 90963861 | ID=MS.gene40279.t1.exon4;Parent=MS.gene40279.t1 |
| chr3.3 | CDS | 90963861 | 90963888 | 90963861 | ID=cds.MS.gene40279.t1;Parent=MS.gene40279.t1 |
| Gene Sequence |
| Protein sequence |
