AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40394.t1 MTR_7g095140 81.572 369 62 2 1 366 1 366 0.0 630
MS.gene40394.t1 MTR_1g072130 46.131 336 158 4 10 324 913 1246 1.34e-98 320
MS.gene40394.t1 MTR_1g103830 39.739 307 162 4 11 296 706 1010 1.76e-72 245
MS.gene40394.t1 MTR_2g078040 36.570 309 175 5 8 298 861 1166 7.72e-61 212
MS.gene40394.t1 MTR_5g071340 34.675 323 184 8 8 309 779 1095 1.24e-57 202
MS.gene40394.t1 MTR_3g464550 31.845 336 197 8 10 323 704 1029 5.36e-55 194
MS.gene40394.t1 MTR_5g024390 33.333 282 163 6 13 278 125 397 6.01e-48 168
MS.gene40394.t1 MTR_5g024500 32.624 282 165 6 13 278 125 397 1.01e-46 164
MS.gene40394.t1 MTR_7g109640 31.973 294 163 7 13 278 481 765 4.82e-42 157
MS.gene40394.t1 MTR_3g103110 29.430 316 196 6 9 304 489 797 1.75e-41 155
MS.gene40394.t1 MTR_3g103120 29.968 317 175 7 9 295 522 821 9.55e-40 150
MS.gene40394.t1 MTR_7g102610 25.683 366 186 11 13 298 588 947 2.76e-28 117
MS.gene40394.t1 MTR_1g068930 26.836 354 180 13 13 299 516 857 2.10e-27 114
MS.gene40394.t1 MTR_1g068930 26.836 354 180 13 13 299 553 894 2.42e-27 114
MS.gene40394.t1 MTR_4g099530 28.616 318 189 11 16 313 215 514 8.60e-27 111
MS.gene40394.t1 MTR_1g062330 24.863 366 193 11 13 300 443 804 1.22e-24 105
MS.gene40394.t1 MTR_4g056990 24.294 354 201 12 6 298 272 619 5.39e-22 97.8
MS.gene40394.t1 MTR_4g050090 22.449 392 205 13 13 318 279 657 1.44e-20 94.0
MS.gene40394.t1 MTR_3g103160 34.965 143 89 2 136 278 276 414 7.55e-20 90.9
MS.gene40394.t1 MTR_4g057290 22.857 350 181 10 6 273 172 514 9.02e-19 88.2
MS.gene40394.t1 MTR_2g084425 29.221 154 100 4 148 298 871 1018 5.36e-14 73.9
MS.gene40394.t1 MTR_2g084425 47.917 48 25 0 13 60 552 599 2.48e-11 65.9
MS.gene40394.t1 MTR_2g084425 47.917 48 25 0 13 60 552 599 2.64e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40394.t1 AT5G36740 40.968 310 164 4 11 303 647 954 4.45e-80 266
MS.gene40394.t1 AT5G36740 40.968 310 164 4 11 303 647 954 4.45e-80 266
MS.gene40394.t1 AT5G36740 39.375 320 165 5 11 303 494 811 3.96e-76 254
MS.gene40394.t1 AT1G05380 38.907 311 166 5 16 308 627 931 3.00e-72 244
MS.gene40394.t1 AT1G05380 38.907 311 166 5 16 308 627 931 3.00e-72 244
MS.gene40394.t1 AT1G05380 38.907 311 166 5 16 308 627 931 3.00e-72 244
MS.gene40394.t1 AT4G14920 38.065 310 168 7 8 298 679 983 2.37e-70 238
MS.gene40394.t1 AT4G14920 38.065 310 168 7 8 298 679 983 2.37e-70 238
MS.gene40394.t1 AT4G14920 38.065 310 168 7 8 298 679 983 2.40e-70 238
MS.gene40394.t1 AT5G36670 37.730 326 166 6 11 303 647 968 5.02e-70 238
MS.gene40394.t1 AT5G36670 37.730 326 166 6 11 303 647 968 5.02e-70 238
MS.gene40394.t1 AT5G36670 36.310 336 167 7 11 303 494 825 6.16e-66 226
MS.gene40394.t1 AT5G36670 36.310 336 167 7 11 303 647 978 8.81e-66 226
MS.gene40394.t1 AT5G36740 42.169 249 125 4 11 242 647 893 3.02e-64 220
MS.gene40394.t1 AT5G36740 42.169 249 125 4 11 242 647 893 3.02e-64 220
MS.gene40394.t1 AT5G36670 42.169 249 125 4 11 242 647 893 3.02e-64 220
MS.gene40394.t1 AT3G14980 36.538 312 175 6 11 304 723 1029 6.86e-59 206
MS.gene40394.t1 AT3G14980 36.538 312 175 6 11 304 723 1029 6.86e-59 206
MS.gene40394.t1 AT3G14980 36.538 312 175 6 11 304 723 1029 6.86e-59 206
MS.gene40394.t1 AT3G14980 36.538 312 175 6 11 304 723 1029 8.98e-59 205
MS.gene40394.t1 AT3G14980 36.538 312 175 6 11 304 678 984 9.57e-59 206
MS.gene40394.t1 AT5G58610 38.127 299 161 8 2 293 682 963 1.93e-54 193
MS.gene40394.t1 AT5G58610 38.127 299 161 8 2 293 682 963 2.45e-54 192
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 460 767 6.80e-54 191
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 691 998 2.02e-53 190
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 682 989 2.16e-53 190
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 691 998 2.48e-53 190
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 691 998 2.48e-53 190
MS.gene40394.t1 AT5G58610 35.873 315 172 9 2 293 682 989 3.25e-53 189
MS.gene40394.t1 AT5G58610 33.955 268 152 7 2 246 691 956 2.55e-42 158
MS.gene40394.t1 AT5G58610 33.955 268 152 7 2 246 682 947 2.84e-42 157
MS.gene40394.t1 AT5G63900 32.698 315 185 12 11 309 253 556 1.46e-38 145
MS.gene40394.t1 AT2G36720 26.203 374 194 10 13 308 614 983 9.71e-36 139
MS.gene40394.t1 AT2G36720 26.203 374 194 10 13 308 614 983 9.71e-36 139
MS.gene40394.t1 AT2G37520 27.809 356 184 11 13 300 467 817 4.66e-34 134
MS.gene40394.t1 AT3G14980 35.945 217 116 6 11 209 723 934 4.85e-33 130
MS.gene40394.t1 AT2G27980 26.260 377 188 14 6 298 687 1057 1.52e-31 126
MS.gene40394.t1 AT3G53680 26.519 362 183 13 13 302 485 835 9.52e-30 121
MS.gene40394.t1 AT3G53680 26.519 362 183 13 13 302 487 837 1.06e-29 121
MS.gene40394.t1 AT5G58610 30.870 230 134 7 2 208 682 909 1.07e-27 115

Find 67 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACTTGGACTTCCTCTTT+TGG 0.216281 7.2:-14592542 MS.gene40394:CDS
CCAATGGCTACTTAGAATTC+TGG 0.217920 7.2:+14594222 None:intergenic
ATGGCAGAGATGCCATTTAT+TGG 0.258182 7.2:-14592916 MS.gene40394:CDS
CTAACTCGTGTTCCAATAAA+TGG 0.259990 7.2:+14592904 None:intergenic
TTGGAACACGAGTTAGGTTT+AGG 0.320138 7.2:-14592897 MS.gene40394:CDS
AAACCCATTACTTTGGATCT+TGG 0.343252 7.2:-14594268 MS.gene40394:CDS
AATTGGTGAAATAGGATTCA+TGG 0.360966 7.2:-14592946 MS.gene40394:intron
CACCGCATGCATCCTCATTA+AGG 0.362137 7.2:+14595677 None:intergenic
GGAAGTGAAAATCCCGCTAT+TGG 0.378386 7.2:-14595020 MS.gene40394:CDS
CATTTATTGGAACACGAGTT+AGG 0.385764 7.2:-14592903 MS.gene40394:CDS
ATTGTCAGTGGAAGAACTTC+AGG 0.387079 7.2:-14592067 MS.gene40394:CDS
CAATGGCTACTTAGAATTCT+GGG 0.405998 7.2:+14594223 None:intergenic
CGCTACAAGTGTTACACACT+TGG 0.417196 7.2:-14592556 MS.gene40394:CDS
CGAGCTCCTCGTAATTCATA+TGG 0.419830 7.2:+14592025 None:intergenic
TTTACTGTATTTCTACAGAA+AGG 0.425583 7.2:-14593084 MS.gene40394:CDS
CCTCCAAGATCCAAAGTAAT+GGG 0.432064 7.2:+14594265 None:intergenic
AGAGAAAGTTTATCAACAAT+CGG 0.432642 7.2:+14591973 None:intergenic
CCCATTACTTTGGATCTTGG+AGG 0.440114 7.2:-14594265 MS.gene40394:CDS
TTACTGTATTTCTACAGAAA+GGG 0.442791 7.2:-14593083 MS.gene40394:CDS
AGGAGCTTCCAGCAGGTGAC+TGG 0.452037 7.2:-14595088 MS.gene40394:CDS
TAGGATTCATGGACATCAAA+TGG 0.466229 7.2:-14592935 MS.gene40394:CDS
CAATCTGTGTTGCTACTCTC+AGG 0.469065 7.2:-14593053 MS.gene40394:intron
GGATCTTGGAGGTGAGAAGA+TGG 0.471084 7.2:-14594254 MS.gene40394:CDS
CAAGACTCAGAAAGCTATCA+AGG 0.471221 7.2:-14592116 MS.gene40394:CDS
TCCTTGCAGGAGCTTCCAGC+AGG 0.473548 7.2:-14595095 MS.gene40394:intron
CAAAATCTCACTTTCAATCA+AGG 0.485189 7.2:-14592407 MS.gene40394:intron
GTTTGAACTCACCGACTCAA+TGG 0.485586 7.2:+14592843 None:intergenic
TCCCGTGCTGTTGATTATGC+CGG 0.486765 7.2:-14593111 MS.gene40394:CDS
ATGCAGTGCCAGTCACCTGC+TGG 0.507073 7.2:+14595080 None:intergenic
AGGTCTTGAAACACTCTTTG+AGG 0.507558 7.2:+14595697 None:intergenic
TTTGCGTAGATTACATGAAG+TGG 0.508611 7.2:-14594335 MS.gene40394:CDS
CAACTTTGATGAAATACTGC+TGG 0.514841 7.2:+14595613 None:intergenic
ACCTCCAAGATCCAAAGTAA+TGG 0.516234 7.2:+14594264 None:intergenic
CGAGTTAGGTTTAGGAAACA+AGG 0.521835 7.2:-14592889 MS.gene40394:CDS
ACATGAAGTGGAAAACAAGA+AGG 0.524527 7.2:-14594323 MS.gene40394:CDS
AATAGATATGACATTGGAGA+TGG 0.525905 7.2:-14594373 MS.gene40394:CDS
CCAGAATTCTAAGTAGCCAT+TGG 0.533832 7.2:-14594222 MS.gene40394:intron
GAGAAAGTTTATCAACAATC+GGG 0.534292 7.2:+14591974 None:intergenic
AGCTTTCTCAAATCCAAAAG+AGG 0.538189 7.2:+14592529 None:intergenic
AGGCACAAACTTTAATGTTG+AGG 0.538417 7.2:+14595590 None:intergenic
AGAGGTTTGAAATGCTTCTA+CGG 0.542161 7.2:-14594396 MS.gene40394:CDS
CTATATGAATGTGGAGATGC+TGG 0.542228 7.2:-14592591 MS.gene40394:CDS
TACTGTATTTCTACAGAAAG+GGG 0.542304 7.2:-14593082 MS.gene40394:CDS
ACGATTGAACTCATCACCGT+TGG 0.545711 7.2:+14594673 None:intergenic
TTACGAGGAGCTCGTGAGAG+AGG 0.547515 7.2:-14592016 MS.gene40394:CDS
TACGAGGAGCTCGTGAGAGA+GGG 0.549642 7.2:-14592015 MS.gene40394:CDS
GCATGCGGTGTTTGTAAAGA+CGG 0.564702 7.2:-14595664 MS.gene40394:CDS
TGTAACACTTGTAGCGCTGA+TGG 0.581027 7.2:+14592563 None:intergenic
CTGAAGACACATCCAATAGC+GGG 0.584552 7.2:+14595008 None:intergenic
CAATGGCATTGAAAAGTCTA+CGG 0.587830 7.2:+14592860 None:intergenic
AGTGTTTCAAGACCTTAATG+AGG 0.588170 7.2:-14595689 MS.gene40394:CDS
TCCGGCATAATCAACAGCAC+GGG 0.592499 7.2:+14593110 None:intergenic
ATCCGGCATAATCAACAGCA+CGG 0.595394 7.2:+14593109 None:intergenic
TTCAATCGTGAAAAGTTCTG+TGG 0.597157 7.2:-14594659 MS.gene40394:CDS
GGCATTAAGCTATATGAATG+TGG 0.599720 7.2:-14592600 MS.gene40394:CDS
AGAGAGTCTTGTATTGTCAG+TGG 0.611720 7.2:-14592079 MS.gene40394:CDS
CATGAAGTGGAAAACAAGAA+GGG 0.614500 7.2:-14594322 MS.gene40394:CDS
CTACGGAATAGATATGACAT+TGG 0.617609 7.2:-14594379 MS.gene40394:CDS
AATGGCTACTTAGAATTCTG+GGG 0.631109 7.2:+14594224 None:intergenic
TGTGGTGCAATTCTATGGAG+TGG 0.632201 7.2:-14592471 MS.gene40394:CDS
TGTGTTTCCAGATACATCAG+AGG 0.640085 7.2:-14594414 MS.gene40394:intron
TGCGGTGTTTGTAAAGACGG+AGG 0.684277 7.2:-14595661 MS.gene40394:CDS
GTCAATTGTGTTGATGCCAA+CGG 0.685380 7.2:-14594689 MS.gene40394:CDS
ACTGAAGACACATCCAATAG+CGG 0.687331 7.2:+14595007 None:intergenic
GACCTTAATGAGGATGCATG+CGG 0.693617 7.2:-14595679 MS.gene40394:CDS
AGAAAGCTATCAAGGAAGAG+CGG 0.699589 7.2:-14592108 MS.gene40394:CDS
AGTTCTCCATATGAATTACG+AGG 0.736915 7.2:-14592031 MS.gene40394:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTTTTATTTAATTT+AGG - chr7.2:14594910-14594929 MS.gene40394:intron 0.0%
!!! TTTAATTTTTATTTAATTTA+GGG - chr7.2:14594911-14594930 MS.gene40394:intron 0.0%
!! TAAAGTCTATTTAATTAAAT+AGG + chr7.2:14594026-14594045 None:intergenic 10.0%
!! TTAAGTATTAATATATCATT+AGG + chr7.2:14592421-14592440 None:intergenic 10.0%
!! TTATCTAATAAAATAGTTAT+CGG + chr7.2:14594321-14594340 None:intergenic 10.0%
!!! ATACTAATTTAACTTAATTT+TGG - chr7.2:14592460-14592479 MS.gene40394:CDS 10.0%
!!! TACTAATTTAACTTAATTTT+GGG - chr7.2:14592461-14592480 MS.gene40394:CDS 10.0%
!! AAAAATAAATCAATACAAAC+AGG - chr7.2:14592326-14592345 MS.gene40394:intron 15.0%
!! AATATAGTTATAAAAAAGAG+TGG + chr7.2:14593503-14593522 None:intergenic 15.0%
!! AGTTAAATTATTTCAATATG+TGG + chr7.2:14592535-14592554 None:intergenic 15.0%
!! ATATTCAACAATATCAATAA+TGG - chr7.2:14593919-14593938 MS.gene40394:intron 15.0%
!! TAATTATGGAGTTTATTAAT+TGG - chr7.2:14594731-14594750 MS.gene40394:intron 15.0%
!! TATAATTAGAAACTTCTTAA+TGG + chr7.2:14594368-14594387 None:intergenic 15.0%
!! TCATTATATGTTAATTACAT+TGG - chr7.2:14592392-14592411 MS.gene40394:intron 15.0%
!! TGCTGTAAAAAAAAATTAAA+TGG + chr7.2:14594396-14594415 None:intergenic 15.0%
!!! AAACATGTAATTTTTGAAAT+AGG + chr7.2:14593691-14593710 None:intergenic 15.0%
!!! AAAGTTTTGTTTATTTTTGA+TGG + chr7.2:14592828-14592847 None:intergenic 15.0%
!!! AAGTTTATTTTCATTATTTG+AGG - chr7.2:14592176-14592195 MS.gene40394:intron 15.0%
!!! AAGTTTTGTTTATTTTTGAT+GGG + chr7.2:14592827-14592846 None:intergenic 15.0%
!!! AGATATTTTGAATAAGTTTA+TGG - chr7.2:14594342-14594361 MS.gene40394:CDS 15.0%
!!! TATACTTTTCTATTTTCTTT+TGG + chr7.2:14595256-14595275 None:intergenic 15.0%
!!! TGTTGTAGATATTTTTTTAT+TGG - chr7.2:14594527-14594546 MS.gene40394:intron 15.0%
!!! TTTTTTTAAGTAGAGTAAAA+AGG + chr7.2:14595032-14595051 None:intergenic 15.0%
!! AAAAAATAAACCAATCCAAA+TGG + chr7.2:14592202-14592221 None:intergenic 20.0%
!! AAAAGATATGATTATGAGAA+AGG + chr7.2:14594153-14594172 None:intergenic 20.0%
!! AAAGATATGATTATGAGAAA+GGG + chr7.2:14594152-14594171 None:intergenic 20.0%
!! AATGCTAAGAAACTAAATAT+AGG - chr7.2:14594935-14594954 MS.gene40394:intron 20.0%
!! AGTTTATTAATTGGTGAAAT+AGG - chr7.2:14594740-14594759 MS.gene40394:intron 20.0%
!! GTCGAAAAACATATTAATTA+TGG - chr7.2:14594717-14594736 MS.gene40394:intron 20.0%
!! TAAAAACACAAATAAGATGA+TGG + chr7.2:14594261-14594280 None:intergenic 20.0%
!! TAAATAACCAAATCATAAGA+AGG - chr7.2:14593877-14593896 MS.gene40394:intron 20.0%
!! TAACGATCAAAGTTATTTAA+AGG + chr7.2:14595488-14595507 None:intergenic 20.0%
!! TAGAAATACAGTAAAAAATC+CGG + chr7.2:14594605-14594624 None:intergenic 20.0%
!! TATTCAGTTAATTAATGAAG+AGG + chr7.2:14592776-14592795 None:intergenic 20.0%
!! TTATTTAGTAGCTGATATAT+TGG + chr7.2:14593864-14593883 None:intergenic 20.0%
!!! AGTTTTGTTTATTTTTGATG+GGG + chr7.2:14592826-14592845 None:intergenic 20.0%
!!! TATACATGTACATTTTTTCT+AGG - chr7.2:14595333-14595352 MS.gene40394:intron 20.0%
! AAAAATTACATGTTTGAGTC+TGG - chr7.2:14593696-14593715 MS.gene40394:intron 25.0%
! AAGATATGATTATGAGAAAG+GGG + chr7.2:14594151-14594170 None:intergenic 25.0%
! AAGCTCAAATCAATACTTAA+AGG - chr7.2:14594183-14594202 MS.gene40394:intron 25.0%
! AGAGACAAGTTAATAAGTTT+AGG + chr7.2:14594876-14594895 None:intergenic 25.0%
! AGTCTATTCAAAATCTAACT+CGG + chr7.2:14594089-14594108 None:intergenic 25.0%
! ATTGCTGAATAAGAAAAATG+TGG - chr7.2:14595205-14595224 MS.gene40394:intron 25.0%
! CTCTTTATCTTGTGTATTTA+GGG - chr7.2:14594992-14595011 MS.gene40394:CDS 25.0%
! GAAATATCCATCTACATTAT+CGG - chr7.2:14593558-14593577 MS.gene40394:intron 25.0%
! GTAGTCATAATCATAGATTA+AGG + chr7.2:14592127-14592146 None:intergenic 25.0%
! TAATTAACACGTAAGAAAAC+TGG + chr7.2:14594285-14594304 None:intergenic 25.0%
! TATGTTCTTATTATCTTGTC+AGG - chr7.2:14594554-14594573 MS.gene40394:intron 25.0%
!! TCATACGATTTTTAATAGCA+GGG + chr7.2:14593828-14593847 None:intergenic 25.0%
!! TTACTGTATTTCTACAGAAA+GGG - chr7.2:14594611-14594630 MS.gene40394:intron 25.0%
!! TTCATACGATTTTTAATAGC+AGG + chr7.2:14593829-14593848 None:intergenic 25.0%
!! TTTACTGTATTTCTACAGAA+AGG - chr7.2:14594610-14594629 MS.gene40394:intron 25.0%
!!! CTCTAAAATGATAAGATCAA+AGG + chr7.2:14592352-14592371 None:intergenic 25.0%
AAAAATGTGGTGCAATTCTA+TGG - chr7.2:14595218-14595237 MS.gene40394:intron 30.0%
AACTCGTTCAATAAAAAGAC+TGG + chr7.2:14594063-14594082 None:intergenic 30.0%
AATAGATATGACATTGGAGA+TGG - chr7.2:14593321-14593340 MS.gene40394:intron 30.0%
AATTGGTGAAATAGGATTCA+TGG - chr7.2:14594748-14594767 MS.gene40394:intron 30.0%
ACCTTTATGATGCATTGAAA+AGG - chr7.2:14592911-14592930 MS.gene40394:CDS 30.0%
ACTCGTTCAATAAAAAGACT+GGG + chr7.2:14594062-14594081 None:intergenic 30.0%
AGAAGAAGAGAAAATACATC+TGG + chr7.2:14592732-14592751 None:intergenic 30.0%
ATTCAAGCCTTCTTATGATT+TGG + chr7.2:14593887-14593906 None:intergenic 30.0%
CAAAATCTCACTTTCAATCA+AGG - chr7.2:14595287-14595306 MS.gene40394:intron 30.0%
CATGATGCTCTACAAAAAAT+TGG - chr7.2:14593590-14593609 MS.gene40394:intron 30.0%
GCTCTTTATCTTGTGTATTT+AGG - chr7.2:14594991-14595010 MS.gene40394:CDS 30.0%
TCTCGTCGCTAAAATTTAAA+TGG - chr7.2:14593154-14593173 MS.gene40394:intron 30.0%
TCTTACGTGTTAATTACTAG+TGG - chr7.2:14594289-14594308 MS.gene40394:CDS 30.0%
! AGGTATGTATACATTTTACG+TGG - chr7.2:14595307-14595326 MS.gene40394:intron 30.0%
! AGTCTTTTTATTGAACGAGT+TGG - chr7.2:14594062-14594081 MS.gene40394:intron 30.0%
! ATCATGCCTTTAAAAATGCT+AGG + chr7.2:14593758-14593777 None:intergenic 30.0%
! ATTTTCACTTCCTTCTTGAT+AGG + chr7.2:14592666-14592685 None:intergenic 30.0%
! GCTTAACCTAGCATTTTTAA+AGG - chr7.2:14593749-14593768 MS.gene40394:intron 30.0%
! TACTGTATTTCTACAGAAAG+GGG - chr7.2:14594612-14594631 MS.gene40394:intron 30.0%
! TCCTTTTCAATGCATCATAA+AGG + chr7.2:14592915-14592934 None:intergenic 30.0%
! TTGAAAGTGAGATTTTGTTC+AGG + chr7.2:14595284-14595303 None:intergenic 30.0%
! TTGTCATAGACTCATTTTCA+AGG - chr7.2:14593727-14593746 MS.gene40394:intron 30.0%
! TTTCATTATTTGAGGCCATT+TGG - chr7.2:14592184-14592203 MS.gene40394:intron 30.0%
! TTTTGAATAGACTAGACTGT+AGG - chr7.2:14594096-14594115 MS.gene40394:intron 30.0%
!! CTTCATAAGTTGTTTTGCAT+AGG - chr7.2:14592300-14592319 MS.gene40394:intron 30.0%
!! GTGTTTTAAACCCATTACTT+TGG - chr7.2:14593419-14593438 MS.gene40394:intron 30.0%
!!! TTTTGAAATAGGTCAGACTT+AGG + chr7.2:14593680-14593699 None:intergenic 30.0%
AAACCCATTACTTTGGATCT+TGG - chr7.2:14593426-14593445 MS.gene40394:intron 35.0%
AATGGCTACTTAGAATTCTG+GGG + chr7.2:14593473-14593492 None:intergenic 35.0%
ACATGAAGTGGAAAACAAGA+AGG - chr7.2:14593371-14593390 MS.gene40394:intron 35.0%
AGCTTTCTCAAATCCAAAAG+AGG + chr7.2:14595168-14595187 None:intergenic 35.0%
AGGCACAAACTTTAATGTTG+AGG + chr7.2:14592107-14592126 None:intergenic 35.0%
AGTGTTTCAAGACCTTAATG+AGG - chr7.2:14592005-14592024 MS.gene40394:CDS 35.0%
AGTTCTCCATATGAATTACG+AGG - chr7.2:14595663-14595682 MS.gene40394:CDS 35.0%
ATTCTAAGTAGCCATTGGTA+AGG - chr7.2:14593477-14593496 MS.gene40394:intron 35.0%
CAACTTTGATGAAATACTGC+TGG + chr7.2:14592084-14592103 None:intergenic 35.0%
CAATGGCTACTTAGAATTCT+GGG + chr7.2:14593474-14593493 None:intergenic 35.0%
CATGAAGTGGAAAACAAGAA+GGG - chr7.2:14593372-14593391 MS.gene40394:intron 35.0%
CATTTATTGGAACACGAGTT+AGG - chr7.2:14594791-14594810 MS.gene40394:intron 35.0%
CTAACTCGTGTTCCAATAAA+TGG + chr7.2:14594793-14594812 None:intergenic 35.0%
CTACGGAATAGATATGACAT+TGG - chr7.2:14593315-14593334 MS.gene40394:intron 35.0%
GGCATTAAGCTATATGAATG+TGG - chr7.2:14595094-14595113 MS.gene40394:intron 35.0%
TAACATCACAATGATTCGAG+TGG + chr7.2:14594223-14594242 None:intergenic 35.0%
TAGGATTCATGGACATCAAA+TGG - chr7.2:14594759-14594778 MS.gene40394:intron 35.0%
TGAGTAACCGATAATGTAGA+TGG + chr7.2:14593568-14593587 None:intergenic 35.0%
TTCAATCGTGAAAAGTTCTG+TGG - chr7.2:14593035-14593054 MS.gene40394:intron 35.0%
TTTCAAACCTCTGATGTATC+TGG + chr7.2:14593290-14593309 None:intergenic 35.0%
TTTGCGTAGATTACATGAAG+TGG - chr7.2:14593359-14593378 MS.gene40394:intron 35.0%
! ACTTTTCAATGCCATTGAGT+CGG - chr7.2:14594840-14594859 MS.gene40394:intron 35.0%
! AGAGGTTTGAAATGCTTCTA+CGG - chr7.2:14593298-14593317 MS.gene40394:intron 35.0%
! ATAACATTCGATGTATCGAC+TGG - chr7.2:14594481-14594500 MS.gene40394:intron 35.0%
! CAATGGCATTGAAAAGTCTA+CGG + chr7.2:14594837-14594856 None:intergenic 35.0%
! CACTGCTCTTGTAAATTTTG+TGG - chr7.2:14592638-14592657 MS.gene40394:intron 35.0%
! TCGAATGTTATTTGCTTCGA+AGG + chr7.2:14594472-14594491 None:intergenic 35.0%
! TTATTTGAGGCCATTTGGAT+TGG - chr7.2:14592189-14592208 MS.gene40394:intron 35.0%
! TTTTGTGGATCCTATCAAGA+AGG - chr7.2:14592653-14592672 MS.gene40394:intron 35.0%
!!! ACATTTTTTCTAGGAGCCAT+GGG - chr7.2:14595342-14595361 MS.gene40394:intron 35.0%
!!! CTTCTTCATTTTTTCCTTGC+AGG - chr7.2:14592586-14592605 MS.gene40394:CDS 35.0%
!!! TACATTTTTTCTAGGAGCCA+TGG - chr7.2:14595341-14595360 MS.gene40394:intron 35.0%
AATGCATCATAAAGGTGACG+AGG + chr7.2:14592907-14592926 None:intergenic 40.0%
AATGCCTGCAGATATATGCA+TGG + chr7.2:14595080-14595099 None:intergenic 40.0%
ACCTCCAAGATCCAAAGTAA+TGG + chr7.2:14593433-14593452 None:intergenic 40.0%
ACTGAAGACACATCCAATAG+CGG + chr7.2:14592690-14592709 None:intergenic 40.0%
AGAAAGCTATCAAGGAAGAG+CGG - chr7.2:14595586-14595605 MS.gene40394:intron 40.0%
AGGTCTTGAAACACTCTTTG+AGG + chr7.2:14592000-14592019 None:intergenic 40.0%
ATTGTCAGTGGAAGAACTTC+AGG - chr7.2:14595627-14595646 MS.gene40394:CDS 40.0%
CAACATCTCATCTCCTAGTA+GGG - chr7.2:14593618-14593637 MS.gene40394:intron 40.0%
CAAGACTCAGAAAGCTATCA+AGG - chr7.2:14595578-14595597 MS.gene40394:intron 40.0%
CCAATGGCTACTTAGAATTC+TGG + chr7.2:14593475-14593494 None:intergenic 40.0%
CCAGAATTCTAAGTAGCCAT+TGG - chr7.2:14593472-14593491 MS.gene40394:intron 40.0%
CCTCCAAGATCCAAAGTAAT+GGG + chr7.2:14593432-14593451 None:intergenic 40.0%
CGAGTTAGGTTTAGGAAACA+AGG - chr7.2:14594805-14594824 MS.gene40394:intron 40.0%
CTATATGAATGTGGAGATGC+TGG - chr7.2:14595103-14595122 MS.gene40394:intron 40.0%
CTCCTAGTAGGGATGAAAAT+AGG - chr7.2:14593629-14593648 MS.gene40394:intron 40.0%
CTGTGGAAAAAGATGTCAAC+AGG - chr7.2:14593052-14593071 MS.gene40394:intron 40.0%
GGATGAAAATAGGCTAAGCT+GGG - chr7.2:14593639-14593658 MS.gene40394:intron 40.0%
TGTGTTTCCAGATACATCAG+AGG - chr7.2:14593280-14593299 MS.gene40394:intron 40.0%
TTGGAACACGAGTTAGGTTT+AGG - chr7.2:14594797-14594816 MS.gene40394:intron 40.0%
! AAAAAGAGTGGCCTTACCAA+TGG + chr7.2:14593491-14593510 None:intergenic 40.0%
! AGAGAGTCTTGTATTGTCAG+TGG - chr7.2:14595615-14595634 MS.gene40394:CDS 40.0%
! AGCCTATTTTCATCCCTACT+AGG + chr7.2:14593634-14593653 None:intergenic 40.0%
! ATGGCAGAGATGCCATTTAT+TGG - chr7.2:14594778-14594797 MS.gene40394:intron 40.0%
! GTCAATTGTGTTGATGCCAA+CGG - chr7.2:14593005-14593024 MS.gene40394:intron 40.0%
! GTGATTTTCATGTTAGCCCA+TGG + chr7.2:14595361-14595380 None:intergenic 40.0%
AAAATAGGCTAAGCTGGGTC+GGG - chr7.2:14593644-14593663 MS.gene40394:intron 45.0%
ACGATTGAACTCATCACCGT+TGG + chr7.2:14593024-14593043 None:intergenic 45.0%
ATCCGGCATAATCAACAGCA+CGG + chr7.2:14594588-14594607 None:intergenic 45.0%
CCAACATCTCATCTCCTAGT+AGG - chr7.2:14593617-14593636 MS.gene40394:intron 45.0%
CCCATTACTTTGGATCTTGG+AGG - chr7.2:14593429-14593448 MS.gene40394:intron 45.0%
CCTACTAGGAGATGAGATGT+TGG + chr7.2:14593620-14593639 None:intergenic 45.0%
CGAGCTCCTCGTAATTCATA+TGG + chr7.2:14595672-14595691 None:intergenic 45.0%
CGCTACAAGTGTTACACACT+TGG - chr7.2:14595138-14595157 MS.gene40394:intron 45.0%
CTGAAGACACATCCAATAGC+GGG + chr7.2:14592689-14592708 None:intergenic 45.0%
GAAAATAGGCTAAGCTGGGT+CGG - chr7.2:14593643-14593662 MS.gene40394:intron 45.0%
GAATAGACTAGACTGTAGGC+CGG - chr7.2:14594100-14594119 MS.gene40394:intron 45.0%
GACCTTAATGAGGATGCATG+CGG - chr7.2:14592015-14592034 MS.gene40394:CDS 45.0%
GCATGCGGTGTTTGTAAAGA+CGG - chr7.2:14592030-14592049 MS.gene40394:CDS 45.0%
GCTACCATGCATATATCTGC+AGG - chr7.2:14595073-14595092 MS.gene40394:CDS 45.0%
GGAAGTGAAAATCCCGCTAT+TGG - chr7.2:14592674-14592693 MS.gene40394:intron 45.0%
GGGATGAAAATAGGCTAAGC+TGG - chr7.2:14593638-14593657 MS.gene40394:intron 45.0%
TGTGGTGCAATTCTATGGAG+TGG - chr7.2:14595223-14595242 MS.gene40394:intron 45.0%
! CAATCTGTGTTGCTACTCTC+AGG - chr7.2:14594641-14594660 MS.gene40394:intron 45.0%
! CACACTTGGACTTCCTCTTT+TGG - chr7.2:14595152-14595171 MS.gene40394:intron 45.0%
! TGGGTGACATTTTCACGAGA+AGG - chr7.2:14592480-14592499 MS.gene40394:CDS 45.0%
! TGTAACACTTGTAGCGCTGA+TGG + chr7.2:14595134-14595153 None:intergenic 45.0%
!! ACATTCGATGTATCGACTGG+TGG - chr7.2:14594484-14594503 MS.gene40394:intron 45.0%
!! GTTTGAACTCACCGACTCAA+TGG + chr7.2:14594854-14594873 None:intergenic 45.0%
CACCGCATGCATCCTCATTA+AGG + chr7.2:14592020-14592039 None:intergenic 50.0%
GGATCTTGGAGGTGAGAAGA+TGG - chr7.2:14593440-14593459 MS.gene40394:intron 50.0%
TCCGGCATAATCAACAGCAC+GGG + chr7.2:14594587-14594606 None:intergenic 50.0%
TGCGGTGTTTGTAAAGACGG+AGG - chr7.2:14592033-14592052 MS.gene40394:CDS 50.0%
! TCCCGTGCTGTTGATTATGC+CGG - chr7.2:14594583-14594602 MS.gene40394:intron 50.0%
!! AAGCTGGGTCGGGTTTTGTA+AGG - chr7.2:14593654-14593673 MS.gene40394:intron 50.0%
ACTAGACTGTAGGCCGGTGT+CGG - chr7.2:14594106-14594125 MS.gene40394:intron 55.0%
CATGTCAGACCATCCGACAC+CGG + chr7.2:14594122-14594141 None:intergenic 55.0%
TACGAGGAGCTCGTGAGAGA+GGG - chr7.2:14595679-14595698 MS.gene40394:CDS 55.0%
TTACGAGGAGCTCGTGAGAG+AGG - chr7.2:14595678-14595697 MS.gene40394:CDS 55.0%
ACCTGCTGGAAGCTCCTGCA+AGG + chr7.2:14592603-14592622 None:intergenic 60.0%
AGGAGCTTCCAGCAGGTGAC+TGG - chr7.2:14592606-14592625 MS.gene40394:intron 60.0%
ATGCAGTGCCAGTCACCTGC+TGG + chr7.2:14592617-14592636 None:intergenic 60.0%
TCCTTGCAGGAGCTTCCAGC+AGG - chr7.2:14592599-14592618 MS.gene40394:CDS 60.0%
! GACTGTAGGCCGGTGTCGGA+TGG - chr7.2:14594110-14594129 MS.gene40394:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 14591985 14595731 14591985 ID=MS.gene40394
chr7.2 mRNA 14591985 14595731 14591985 ID=MS.gene40394.t1;Parent=MS.gene40394
chr7.2 exon 14595600 14595731 14595600 ID=MS.gene40394.t1.exon1;Parent=MS.gene40394.t1
chr7.2 CDS 14595600 14595731 14595600 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14594982 14595108 14594982 ID=MS.gene40394.t1.exon2;Parent=MS.gene40394.t1
chr7.2 CDS 14594982 14595108 14594982 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14594643 14594716 14594643 ID=MS.gene40394.t1.exon3;Parent=MS.gene40394.t1
chr7.2 CDS 14594643 14594716 14594643 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14594223 14594425 14594223 ID=MS.gene40394.t1.exon4;Parent=MS.gene40394.t1
chr7.2 CDS 14594223 14594425 14594223 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14593054 14593140 14593054 ID=MS.gene40394.t1.exon5;Parent=MS.gene40394.t1
chr7.2 CDS 14593054 14593140 14593054 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14592855 14592954 14592855 ID=MS.gene40394.t1.exon6;Parent=MS.gene40394.t1
chr7.2 CDS 14592855 14592954 14592855 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14592408 14592621 14592408 ID=MS.gene40394.t1.exon7;Parent=MS.gene40394.t1
chr7.2 CDS 14592408 14592621 14592408 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
chr7.2 exon 14591985 14592151 14591985 ID=MS.gene40394.t1.exon8;Parent=MS.gene40394.t1
chr7.2 CDS 14591985 14592151 14591985 ID=cds.MS.gene40394.t1;Parent=MS.gene40394.t1
Gene Sequence

>MS.gene40394

ATGGATCTGGAACCTCAAAGAGTGTTTCAAGACCTTAATGAGGATGCATGCGGTGTTTGTAAAGACGGAGGAGACTTGATTTGCTGTGATAGTTGTCCAGCAGTATTTCATCAAAGTTGCCTCAACATTAAAGTTTGTGCCTTAATCTATGATTATGACTACTTTTTAGTTTATTTTCATTATTTTTTATTAAGTTTATTTTCATTATTTGAGGCCATTTGGATTGGTTTATTTTTTAGTTTATAAAAAATAGTTTATGCAAATAAATAAGCGATTATGTTGATTCATAATTTCTCACTCATGAAACTTGTTTATCTTCATAAGTTGTTTTGCATAGGATAAAAAATAAATCAATACAAACAGGCCTTTGATCTTATCATTTTAGAGATCATTCTTTTATTTATTTCTCATTATATGTTAATTACATTGGTCACCTAATGATATATTAATACTTAAGAAATTTATTATTTTTCTTATACTAATTTAACTTAATTTTGGGTGACATTTTCACGAGAAGGAAATCAGAAATCTTATTGATATGATAAAGCCACATATTGAAATAATTTAACTTAATTTTGTACTAGAATATGTAAATCATCATCTTCTTCATTTTTTCCTTGCAGGAGCTTCCAGCAGGTGACTGGCACTGCATTCACTGCTCTTGTAAATTTTGTGGATCCTATCAAGAAGGAAGTGAAAATCCCGCTATTGGATGTGTCTTCAGTGCATGTTTTTCATGCAGAACCAGATGTATTTTCTCTTCTTCTTTTTCTTATACTAATATATCTCCTCTTCATTAATTAACTGAATATTCATTTCTCAATAATGTATTTTCTTTCCCCATCAAAAATAAACAAAACTTTTCTGATAAACAATGTGTTAACATCAAACTTAGCATCGTAAGTATATATTATAGTCTCCTCGTCACCTTTATGATGCATTGAAAAGGATATCATGTGTGCTCTATATATATAGTTGAAAGATAAATAAACTAAACTTTATATTTCTTATGTAGATCACGTCAATTGTGTTGATGCCAACGGTGATGAGTTCAATCGTGAAAAGTTCTGTGGAAAAAGATGTCAACAGGTATGATATTTTTTCATATTTTAAAATCTTGATCATCAATTGTTCTGAATTTTGTTAGAGTGATGCAAAGATATTTTTGATCTCGTCGCTAAAATTTAAATGGTTATTTTTTGCAAATGCTCTTTTAGAGAATGTTTTAGAGAAGACACGAGTGCACAGCTATAACAAACTGTTTTTGATAATTGTAATAATTAATAGTTAAGAATTGTGTTTCCAGATACATCAGAGGTTTGAAATGCTTCTACGGAATAGATATGACATTGGAGATGGATTTTCTTGCACATTTTTGCGTAGATTACATGAAGTGGAAAACAAGAAGGGTGTTGCATTATCTGTAATGCATGAGTGTTTTAAACCCATTACTTTGGATCTTGGAGGTGAGAAGATGGACATGTTCCCCAGAATTCTAAGTAGCCATTGGTAAGGCCACTCTTTTTTATAACTATATTTTTTTAAGTAATTTTTTATAACTAATTATTAGTATGAAATATCCATCTACATTATCGGTTACTCATACATGATGCTCTACAAAAAATTGGCTAACCAACATCTCATCTCCTAGTAGGGATGAAAATAGGCTAAGCTGGGTCGGGTTTTGTAAGGCCTAAGTCTGACCTATTTCAAAAATTACATGTTTGAGTCTGGTCTATTACTTGTCATAGACTCATTTTCAAGGCTTAACCTAGCATTTTTAAAGGCATGATGAAACTTGAAAGTCTATTTAAAAGCATATTTTACGTACACTACGTAGCCCTGCTATTAAAAATCGTATGAATGTCTAATCACACCAATATATCAGCTACTAAATAACCAAATCATAAGAAGGCTTGAATTTATGAAAAATAATATTCAACAATATCAATAATGGTAGATATATTATATATAAAAAAAGCTTAATAGACTGAATTGATGTGCTTAATAATTTTTTTTTTTGTAATTCTTAGTCTAGCCTATTTAATTAAATAGACTTTATATAAAGTTTGAGCCCAGTCTTTTTATTGAACGAGTTGGACCGAGTTAGATTTTGAATAGACTAGACTGTAGGCCGGTGTCGGATGGTCTGACATGTTTTCACCCCTTTCTCATAATCATATCTTTTTCATTCGCAAAAGCTCAAATCAATACTTAAAGGATTAAAGTTTAGTTCCACTCGAATCATTGTGATGTTAGTTAATATCATTTTACCATCATCTTATTTGTGTTTTTAACCAGTTTTCTTACGTGTTAATTACTAGTGGCTCACTCCGATAACTATTTTATTAGATAAAAGATATTTTGAATAAGTTTATGGCCATTAAGAAGTTTCTAATTATATATTTCCATTTAATTTTTTTTTACAGCAAATATATTTTCATATATATAATTTACTAAAAGTGTAATGTAACAGATACAGTACCTTCGAAGCAAATAACATTCGATGTATCGACTGGTGGATCTCAAAATCTAATTATCATGTTGTAGATATTTTTTTATTGGTATTTATGTTCTTATTATCTTGTCAGGTCAACATCCCGTGCTGTTGATTATGCCGGATTTTTTACTGTATTTCTACAGAAAGGGGATGATACAATCTGTGTTGCTACTCTCAGGTAAATCTATTTATTTTTTTTATCAATGCTCATGCAACTTATAGAGTAATTACTGTCGAAAAACATATTAATTATGGAGTTTATTAATTGGTGAAATAGGATTCATGGACATCAAATGGCAGAGATGCCATTTATTGGAACACGAGTTAGGTTTAGGAAACAAGGAATGTGCCGTAGACTTTTCAATGCCATTGAGTCGGTGAGTTCAAACCTAAACTTATTAACTTGTCTCTATTTCGTGTATTTAATTTAATTTTTATTTAATTTAGGGAAATGCTAAGAAACTAAATATAGGAATATAAATTTCTCATTCAATTCAATTTTAGTAGCTCTTTATCTTGTGTATTTAGGGCACAAATTAGTTTACCTTTTTACTCTACTTAAAAAAATTATTTATCACTTAATATATTGCTACCATGCATATATCTGCAGGCATTAAGCTATATGAATGTGGAGATGCTGGTCATTCCATCAGCGCTACAAGTGTTACACACTTGGACTTCCTCTTTTGGATTTGAGAAAGCTGACATGACTACAAAAGAATTGCTGAATAAGAAAAATGTGGTGCAATTCTATGGAGTGGAATTTCTCCAAAAGAAAATAGAAAAGTATAATCTTCCTGAACAAAATCTCACTTTCAATCAAGGTATGTATACATTTTACGTGGATATATACATGTACATTTTTTCTAGGAGCCATGGGCTAACATGAAAATCACTATTAGAAAAAGTTGAATTAATAACAAAAAAACAAATATAAAAAATATGAAATCTTATTCTAATTTCGTTACTGTATTTAGTAGTAACAAATTTTATTTTTACGCCTTTAAATAACTTTGATCGTTAATTATATTTTTTTTTCTTAGTAAAATTTGTTTGCTAATCAATGAAACTTTGTTGCAGTCTTCAATTATCACAAGACTCAGAAAGCTATCAAGGAAGAGCGGTCTCTAAGAGAGTCTTGTATTGTCAGTGGAAGAACTTCAGGTCGCAGAAGACGAAGTTCTCCATATGAATTACGAGGAGCTCGTGAGAGAGGGAGTTCATCAAAATCAACGCCCGATTGTTGA
Protein sequence

>MS.gene40394.t1

MDLEPQRVFQDLNEDACGVCKDGGDLICCDSCPAVFHQSCLNIKELPAGDWHCIHCSCKFCGSYQEGSENPAIGCVFSACFSCRTRYHVNCVDANGDEFNREKFCGKRCQQIHQRFEMLLRNRYDIGDGFSCTFLRRLHEVENKKGVALSVMHECFKPITLDLGGEKMDMFPRILSSHWSTSRAVDYAGFFTVFLQKGDDTICVATLRIHGHQMAEMPFIGTRVRFRKQGMCRRLFNAIESALSYMNVEMLVIPSALQVLHTWTSSFGFEKADMTTKELLNKKNVVQFYGVEFLQKKIEKYNLPEQNLTFNQVFNYHKTQKAIKEERSLRESCIVSGRTSGRRRRSSPYELRGARERGSSSKSTPDC