AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40527

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40527.t1 MTR_5g005110 92.169 332 18 2 1 325 1 331 0.0 623
MS.gene40527.t1 MTR_5g005110 91.288 264 15 2 1 257 1 263 1.10e-174 485
MS.gene40527.t1 MTR_1g106460 49.415 342 125 11 1 308 1 328 2.54e-85 260
MS.gene40527.t1 MTR_1g106470 49.355 310 130 9 1 307 1 286 4.13e-77 238
MS.gene40527.t1 MTR_3g103030 50.909 220 92 7 92 306 111 319 2.17e-56 186
MS.gene40527.t1 MTR_1g106460 41.356 295 115 11 1 256 1 276 8.48e-48 162
MS.gene40527.t1 MTR_1g018090 58.491 159 53 4 138 293 201 349 2.54e-44 155
MS.gene40527.t1 MTR_1g106470 42.146 261 125 8 1 259 1 237 4.73e-43 149
MS.gene40527.t1 MTR_1g106480 56.081 148 30 4 192 307 12 156 1.25e-39 137
MS.gene40527.t1 MTR_5g079500 62.500 72 27 0 215 286 215 286 1.36e-25 104
MS.gene40527.t1 MTR_1g018090 49.206 126 51 4 138 260 201 316 5.95e-19 86.3
MS.gene40527.t1 MTR_4g131160 60.000 65 26 0 217 281 195 259 1.06e-18 86.3
MS.gene40527.t1 MTR_4g131160 60.000 65 26 0 217 281 198 262 1.20e-18 86.3
MS.gene40527.t1 MTR_8g093500 66.071 56 19 0 227 282 123 178 2.13e-18 84.0
MS.gene40527.t1 MTR_3g101810 61.111 54 21 0 227 280 180 233 9.15e-17 79.3
MS.gene40527.t1 MTR_7g069590 60.000 55 22 0 225 279 33 87 1.08e-16 76.3
MS.gene40527.t1 MTR_1g080890 58.462 65 27 0 217 281 192 256 1.14e-16 80.1
MS.gene40527.t1 MTR_1g019240 39.604 101 54 1 202 295 62 162 1.49e-15 75.9
MS.gene40527.t1 MTR_3g449770 45.570 79 36 2 211 289 304 375 7.51e-14 72.4
MS.gene40527.t1 MTR_1g084980 46.970 66 35 0 216 281 460 525 1.30e-13 72.0
MS.gene40527.t1 MTR_5g017040 43.939 66 37 0 229 294 116 181 7.98e-13 68.6
MS.gene40527.t1 MTR_4g033295 52.239 67 31 1 217 282 117 183 1.62e-12 65.5
MS.gene40527.t1 MTR_4g033295 54.545 66 29 1 217 281 117 182 1.93e-12 65.5
MS.gene40527.t1 MTR_1g069155 45.455 66 36 0 216 281 315 380 2.13e-12 68.2
MS.gene40527.t1 MTR_5g048860 33.951 162 87 4 128 281 280 429 3.85e-12 67.4
MS.gene40527.t1 MTR_7g053410 51.562 64 30 1 219 281 318 381 1.09e-11 65.9
MS.gene40527.t1 MTR_7g099540 45.588 68 36 1 214 281 249 315 1.23e-11 65.5
MS.gene40527.t1 MTR_7g053410 51.562 64 30 1 219 281 318 381 1.24e-11 65.9
MS.gene40527.t1 MTR_8g062240 50.000 64 31 1 219 281 152 215 1.32e-11 64.3
MS.gene40527.t1 MTR_8g012290 51.562 64 30 1 219 281 292 355 1.43e-11 65.5
MS.gene40527.t1 MTR_8g012290 51.562 64 30 1 219 281 292 355 1.45e-11 65.5
MS.gene40527.t1 MTR_3g116770 42.708 96 46 3 187 281 168 255 2.55e-11 64.3
MS.gene40527.t1 MTR_4g070320 32.520 123 65 2 177 281 85 207 2.62e-11 64.3
MS.gene40527.t1 MTR_3g116770 42.708 96 46 3 187 281 168 255 2.63e-11 64.3
MS.gene40527.t1 MTR_8g065740 42.553 94 35 2 207 281 276 369 2.97e-11 64.3
MS.gene40527.t1 MTR_1g116790 50.000 68 33 1 217 283 264 331 9.38e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40527.t1 AT2G14760 41.390 331 145 13 10 304 6 323 1.46e-61 199
MS.gene40527.t1 AT4G33880 39.542 349 147 11 10 300 6 348 1.86e-58 192
MS.gene40527.t1 AT2G14760 40.580 345 141 15 10 304 6 336 5.84e-58 190
MS.gene40527.t1 AT1G27740 67.544 114 36 1 189 301 137 250 1.67e-47 160
MS.gene40527.t1 AT5G43175 54.140 157 53 5 154 301 69 215 8.59e-45 152
MS.gene40527.t1 AT1G66470 71.667 60 17 0 227 286 204 263 2.55e-24 100
MS.gene40527.t1 AT5G37800 70.000 60 18 0 227 286 210 269 2.60e-24 100
MS.gene40527.t1 AT2G24260 63.077 65 24 0 217 281 34 98 3.89e-21 90.9
MS.gene40527.t1 AT2G24260 63.077 65 24 0 217 281 137 201 2.26e-20 90.9
MS.gene40527.t1 AT4G30980 63.077 65 24 0 217 281 129 193 2.59e-20 90.1
MS.gene40527.t1 AT4G30980 63.077 65 24 0 217 281 177 241 4.87e-20 89.7
MS.gene40527.t1 AT5G58010 58.462 65 27 0 217 281 98 162 2.10e-18 84.3
MS.gene40527.t1 AT5G01305 57.377 61 26 0 217 277 36 96 2.01e-17 79.0
MS.gene40527.t1 AT3G21330 57.143 56 24 0 225 280 276 331 5.02e-16 78.6
MS.gene40527.t1 AT4G00120 51.852 54 26 0 227 280 121 174 3.53e-15 73.6
MS.gene40527.t1 AT2G46970 32.520 123 67 4 201 307 166 288 2.39e-13 70.5
MS.gene40527.t1 AT2G46970 32.520 123 67 4 201 307 175 297 2.56e-13 70.5
MS.gene40527.t1 AT2G46970 32.520 123 67 4 201 307 197 319 2.76e-13 70.5
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 191 270 4.88e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 191 270 4.88e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 191 270 4.88e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 262 341 5.85e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 262 341 5.85e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 262 341 5.85e-13 69.7
MS.gene40527.t1 AT2G20180 45.679 81 43 1 201 281 262 341 5.85e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT1G09530 41.463 82 44 1 213 290 328 409 6.99e-13 69.7
MS.gene40527.t1 AT5G50915 46.753 77 39 2 219 294 137 212 1.66e-12 67.4
MS.gene40527.t1 AT5G50915 46.753 77 39 2 219 294 137 212 1.66e-12 67.4
MS.gene40527.t1 AT5G50915 46.753 77 39 2 219 294 137 212 1.95e-12 67.0
MS.gene40527.t1 AT5G50915 46.753 77 39 2 219 294 137 212 1.95e-12 67.0
MS.gene40527.t1 AT2G20180 45.570 79 42 1 201 279 262 339 1.96e-12 67.8
MS.gene40527.t1 AT4G36930 38.889 90 53 2 225 312 198 287 2.00e-12 67.8
MS.gene40527.t1 AT4G00050 37.607 117 56 4 173 285 69 172 3.99e-12 66.2
MS.gene40527.t1 AT1G73830 36.806 144 74 6 147 281 76 211 4.67e-12 65.5
MS.gene40527.t1 AT1G73830 36.806 144 74 6 147 281 76 211 5.39e-12 65.5
MS.gene40527.t1 AT1G25330 53.125 64 29 1 219 281 105 168 5.67e-12 64.7
MS.gene40527.t1 AT1G59640 43.478 92 44 3 219 309 137 221 5.72e-12 65.5
MS.gene40527.t1 AT4G00050 37.607 117 56 4 173 285 171 274 6.51e-12 66.2
MS.gene40527.t1 AT4G00050 37.607 117 56 4 173 285 171 274 7.08e-12 66.2
MS.gene40527.t1 AT4G09180 50.000 68 33 1 217 283 183 250 1.07e-11 64.7
MS.gene40527.t1 AT1G35460 51.515 66 31 1 217 281 180 245 1.53e-11 64.3
MS.gene40527.t1 AT1G59640 43.011 93 45 3 219 310 137 222 1.56e-11 65.1
MS.gene40527.t1 AT3G07340 51.562 64 30 1 219 281 259 322 1.90e-11 65.1
MS.gene40527.t1 AT3G07340 51.562 64 30 1 219 281 259 322 2.14e-11 64.7
MS.gene40527.t1 AT5G48560 51.562 64 30 1 219 281 302 365 2.20e-11 65.1
MS.gene40527.t1 AT5G48560 51.562 64 30 1 219 281 302 365 2.35e-11 64.7
MS.gene40527.t1 AT1G51140 50.769 65 31 1 217 280 303 367 2.44e-11 64.7
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 173 236 2.45e-11 64.3
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 202 265 2.77e-11 64.3
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 224 287 3.09e-11 64.3
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 224 287 3.09e-11 64.3
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 224 287 3.09e-11 64.3
MS.gene40527.t1 AT1G26260 51.562 64 30 1 219 281 224 287 3.09e-11 64.3
MS.gene40527.t1 AT4G28790 39.326 89 53 1 207 295 260 347 4.69e-11 63.9
MS.gene40527.t1 AT5G67110 50.980 51 25 0 231 281 100 150 4.74e-11 62.0
MS.gene40527.t1 AT4G34530 50.000 64 31 1 219 281 173 236 4.75e-11 63.5
MS.gene40527.t1 AT2G14760 29.771 262 133 14 10 234 6 253 6.01e-11 62.4

Find 63 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACATTCCTCTTGTTCTT+TGG 0.181067 5.2:-411040 None:intergenic
CAGCTTTGACTATATTTCTA+TGG 0.187224 5.2:+410679 MS.gene40527:CDS
TGACATTCCTCTTGTTCTTT+GGG 0.254260 5.2:-411039 None:intergenic
TGTTGTACATGGAGTGAATT+TGG 0.258460 5.2:-410720 None:intergenic
ATTTAGCCGTAGAATCATTC+TGG 0.309924 5.2:-411210 None:intergenic
ATATAGTCAAAGCTGGTTAC+TGG 0.320414 5.2:-410671 None:intergenic
AGTTTCAGTACCCAACAAAT+TGG 0.360695 5.2:-410564 None:intergenic
AGTGAATTTGGAATGATCTA+TGG 0.365652 5.2:-410708 None:intergenic
ACCACAAGCGCAGAAAATTC+AGG 0.383330 5.2:+410833 MS.gene40527:CDS
TATCAAAATTCCAATTTGTT+GGG 0.383401 5.2:+410554 MS.gene40527:CDS
GAAAGTTACCTTAGTTCCAT+TGG 0.390559 5.2:-411701 None:intergenic
AAAGTTACCTTAGTTCCATT+GGG 0.395654 5.2:-411700 None:intergenic
CTATCAAAATTCCAATTTGT+TGG 0.398328 5.2:+410553 MS.gene40527:CDS
CTGAGCTTATTTCTCAAGAA+TGG 0.409782 5.2:+410447 MS.gene40527:CDS
GACTTTATGAACCAGTTACT+TGG 0.418424 5.2:+410506 MS.gene40527:CDS
ACGTAGCTCATGAGAAGAAT+TGG 0.419048 5.2:-411164 None:intergenic
TTCATTCCATTGTAAGCAAT+GGG 0.427456 5.2:-412005 None:intergenic
ATGAGGAGTTACCAAGTAAC+TGG 0.430149 5.2:-410517 None:intergenic
TGGGTACTGAAACTGCATTT+TGG 0.433806 5.2:+410573 MS.gene40527:CDS
GAATACAAGTCCTGGTTCTC+AGG 0.435078 5.2:+411105 MS.gene40527:CDS
GATTCTACGGCTAAATTAAG+TGG 0.448568 5.2:+411217 MS.gene40527:CDS
CCTTAGTTCCATTGGGGACA+AGG 0.451533 5.2:-411693 None:intergenic
CCTTGTCCCCAATGGAACTA+AGG 0.456805 5.2:+411693 MS.gene40527:CDS
GTTCATTCCATTGTAAGCAA+TGG 0.476694 5.2:-412006 None:intergenic
ACCAGGACTTGTATTCTCTT+CGG 0.481215 5.2:-411098 None:intergenic
AGCTTTGACTATATTTCTAT+GGG 0.482058 5.2:+410680 MS.gene40527:CDS
TCATCAGATTGTGTTGTACA+TGG 0.498142 5.2:-410731 None:intergenic
ATGAACATTGGACTTGAACT+TGG 0.504815 5.2:+412023 MS.gene40527:CDS
ACAAGAGGAATGTCAAATGT+AGG 0.531678 5.2:+411047 MS.gene40527:CDS
CATAGAAATATAGTCAAAGC+TGG 0.533612 5.2:-410678 None:intergenic
GCTTACAATGGAATGAACAT+TGG 0.534664 5.2:+412011 MS.gene40527:CDS
ACCGAAGAGAATACAAGTCC+TGG 0.537768 5.2:+411097 MS.gene40527:CDS
ATAGAAATGTTGTGAGAATG+AGG 0.543597 5.2:-410534 None:intergenic
AGGCTCTGTGGATCAGTAGC+AGG 0.543740 5.2:-411262 None:intergenic
TTCTCATGAGCTACGTGGAA+GGG 0.546048 5.2:+411171 MS.gene40527:CDS
TCTTGAGAAATAAGCTCAGT+AGG 0.547757 5.2:-410443 None:intergenic
GAAGAGAAAGAATAAATGAA+AGG 0.551355 5.2:+411653 MS.gene40527:CDS
TACTCTCCCATTGCTTACAA+TGG 0.559351 5.2:+411999 MS.gene40527:CDS
TTTGAGCCAGAATGATTCTA+CGG 0.561478 5.2:+411204 MS.gene40527:CDS
CTACAAAACCTTGTCCCCAA+TGG 0.563548 5.2:+411685 MS.gene40527:CDS
ACTAGTTTCTGAACCAGAAG+AGG 0.570837 5.2:+410805 MS.gene40527:CDS
TATAGGTCCCAAAGAACAAG+AGG 0.573850 5.2:+411032 MS.gene40527:intron
AACGTCTGATCCTGAGAACC+AGG 0.576280 5.2:-411115 None:intergenic
CTTCTCATGAGCTACGTGGA+AGG 0.576985 5.2:+411170 MS.gene40527:CDS
AAGATCTAGGAGCTCAAATG+AGG 0.582801 5.2:+410859 MS.gene40527:CDS
CTTGTATCAGAAGTTGAGGT+AGG 0.584296 5.2:-410632 None:intergenic
TCTCAGGATCAGACGTTAAG+CGG 0.590949 5.2:+411121 MS.gene40527:CDS
GGATATCAGCACCATGCTTG+AGG 0.592275 5.2:+411813 MS.gene40527:CDS
GCAACTTGTATCAGAAGTTG+AGG 0.594320 5.2:-410636 None:intergenic
AACTTGTGCAGTAACAATTG+TGG 0.601327 5.2:-410609 None:intergenic
GTTAAGCGGTTATTGTTCAG+AGG 0.601995 5.2:+411135 MS.gene40527:CDS
TTGGACAGCTTCCTCAAGCA+TGG 0.610000 5.2:-411824 None:intergenic
TACCCCAACAAAAGAAACAA+AGG 0.623892 5.2:+412049 MS.gene40527:CDS
AAAGACTTACTCTTGCATAG+AGG 0.624768 5.2:-411282 None:intergenic
AAGTTACCTTAGTTCCATTG+GGG 0.630156 5.2:-411699 None:intergenic
TTCTAAGCTCTGATGACATG+TGG 0.645185 5.2:+411973 MS.gene40527:CDS
GGAAAATCAAGATCTAGTAG+AGG 0.649455 5.2:+411238 MS.gene40527:CDS
CAGTGGACTCTACACTGCTG+AGG 0.657902 5.2:+410478 MS.gene40527:CDS
ACTCTTGCATAGAGGCTCTG+TGG 0.664485 5.2:-411274 None:intergenic
CAAGAATGGACTTCTCTCAG+TGG 0.692150 5.2:+410461 MS.gene40527:CDS
AATTCTTCTCATGAGCTACG+TGG 0.695539 5.2:+411166 MS.gene40527:CDS
GTAACAATTGTGGAATCATG+TGG 0.707912 5.2:-410599 None:intergenic
ACAATTGTGGAATCATGTGG+TGG 0.728994 5.2:-410596 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTAGAAGATTAATTCA+TGG - chr5.2:410900-410919 None:intergenic 20.0%
!! ACTATATATTGAACAACAAT+TGG + chr5.2:411742-411761 MS.gene40527:intron 20.0%
!! AGAAATAAAAAACAGTAGTA+AGG - chr5.2:410926-410945 None:intergenic 20.0%
!! CATTTAGAAGATTAATTCAT+GGG - chr5.2:410899-410918 None:intergenic 20.0%
!! GAAATAAAAAACAGTAGTAA+GGG - chr5.2:410925-410944 None:intergenic 20.0%
!! TCGATATACAAACATTAAAT+AGG - chr5.2:411357-411376 None:intergenic 20.0%
!!! GTTTTTACAACTTCAAATTA+AGG + chr5.2:411855-411874 MS.gene40527:CDS 20.0%
!!! TATCAAAATTCCAATTTGTT+GGG + chr5.2:410554-410573 MS.gene40527:CDS 20.0%
!!! TCTTTTCTATACAATACAAA+AGG - chr5.2:411635-411654 None:intergenic 20.0%
!!! TTTGAAATTAGAAAACTAGA+AGG - chr5.2:411391-411410 None:intergenic 20.0%
! AAAATTCAGGAAAAAGATCT+AGG + chr5.2:410846-410865 MS.gene40527:CDS 25.0%
! ACCTATAAAATCAAATGACA+CGG - chr5.2:411019-411038 None:intergenic 25.0%
! ATACAATACAAAAGGAAACA+TGG - chr5.2:411627-411646 None:intergenic 25.0%
! CTATCAAAATTCCAATTTGT+TGG + chr5.2:410553-410572 MS.gene40527:CDS 25.0%
! GAAGAGAAAGAATAAATGAA+AGG + chr5.2:411653-411672 MS.gene40527:CDS 25.0%
! GTGCTTATTCATAATGTATT+TGG + chr5.2:411553-411572 MS.gene40527:intron 25.0%
! GTTCCATTCAAATTCATATA+GGG - chr5.2:411950-411969 None:intergenic 25.0%
! GTTGTAAAAACTTCACATAT+TGG - chr5.2:411846-411865 None:intergenic 25.0%
! TGTTCAATATATAGTCTGAA+TGG - chr5.2:411737-411756 None:intergenic 25.0%
! TGTTCCATTCAAATTCATAT+AGG - chr5.2:411951-411970 None:intergenic 25.0%
!! AGCTTTGACTATATTTCTAT+GGG + chr5.2:410680-410699 MS.gene40527:CDS 25.0%
!! TGCATATCTTTTATGACTTA+CGG - chr5.2:411447-411466 None:intergenic 25.0%
!!! ATATCCTTTGTTTCTTTTGT+TGG - chr5.2:412056-412075 None:intergenic 25.0%
!!! ATGTTAATTGTTAAGTTGGT+AGG + chr5.2:411521-411540 MS.gene40527:intron 25.0%
!!! TATCCTTTGTTTCTTTTGTT+GGG - chr5.2:412055-412074 None:intergenic 25.0%
AAAACGAAATGCATATCACT+TGG - chr5.2:410954-410973 None:intergenic 30.0%
AAAATCCAAACCATAAACTG+TGG - chr5.2:410980-410999 None:intergenic 30.0%
AAAGTTACCTTAGTTCCATT+GGG - chr5.2:411703-411722 None:intergenic 30.0%
ATAGAAATGTTGTGAGAATG+AGG - chr5.2:410537-410556 None:intergenic 30.0%
CAACTTCAAATTAAGGTTTG+AGG + chr5.2:411862-411881 MS.gene40527:intron 30.0%
CATAGAAATATAGTCAAAGC+TGG - chr5.2:410681-410700 None:intergenic 30.0%
GATCCCTATATGAATTTGAA+TGG + chr5.2:411944-411963 MS.gene40527:intron 30.0%
GTGGATGTTAATTGTTAAGT+TGG + chr5.2:411517-411536 MS.gene40527:intron 30.0%
TCTAGCTAACAAAAATTTGC+AGG + chr5.2:410760-410779 MS.gene40527:CDS 30.0%
TTCAATCAAGCTCGATTAAA+AGG + chr5.2:411487-411506 MS.gene40527:intron 30.0%
TTCATTCCATTGTAAGCAAT+GGG - chr5.2:412008-412027 None:intergenic 30.0%
! AGTGAATTTGGAATGATCTA+TGG - chr5.2:410711-410730 None:intergenic 30.0%
! CAGCTTTGACTATATTTCTA+TGG + chr5.2:410679-410698 MS.gene40527:CDS 30.0%
! TTTTAAATGCAGAGAGAGAA+AGG - chr5.2:411893-411912 None:intergenic 30.0%
!! ATCCTTTGTTTCTTTTGTTG+GGG - chr5.2:412054-412073 None:intergenic 30.0%
!! TCGATTAAAAGGTGTTTATG+TGG + chr5.2:411498-411517 MS.gene40527:intron 30.0%
!!! TCCGTGTCATTTGATTTTAT+AGG + chr5.2:411015-411034 MS.gene40527:intron 30.0%
AAACATGGTAAGACCTTACA+TGG - chr5.2:411612-411631 None:intergenic 35.0%
AAAGACTTACTCTTGCATAG+AGG - chr5.2:411285-411304 None:intergenic 35.0%
AACTTGTGCAGTAACAATTG+TGG - chr5.2:410612-410631 None:intergenic 35.0%
AAGTTACCTTAGTTCCATTG+GGG - chr5.2:411702-411721 None:intergenic 35.0%
ACAAGAGGAATGTCAAATGT+AGG + chr5.2:411047-411066 MS.gene40527:CDS 35.0%
AGCAACACTTAGTTTCTCAT+AGG + chr5.2:411313-411332 MS.gene40527:intron 35.0%
AGTTTCAGTACCCAACAAAT+TGG - chr5.2:410567-410586 None:intergenic 35.0%
ATAGGACCACAAGATGTATT+TGG - chr5.2:411339-411358 None:intergenic 35.0%
ATATAGTCAAAGCTGGTTAC+TGG - chr5.2:410674-410693 None:intergenic 35.0%
ATGAACATTGGACTTGAACT+TGG + chr5.2:412023-412042 MS.gene40527:CDS 35.0%
ATTTAGCCGTAGAATCATTC+TGG - chr5.2:411213-411232 None:intergenic 35.0%
CAAATGAGGTAACAAACAGA+AGG + chr5.2:410873-410892 MS.gene40527:intron 35.0%
GAAAGTTACCTTAGTTCCAT+TGG - chr5.2:411704-411723 None:intergenic 35.0%
GACTTTATGAACCAGTTACT+TGG + chr5.2:410506-410525 MS.gene40527:CDS 35.0%
GATTCTACGGCTAAATTAAG+TGG + chr5.2:411217-411236 MS.gene40527:CDS 35.0%
GCTTACAATGGAATGAACAT+TGG + chr5.2:412011-412030 MS.gene40527:CDS 35.0%
GGAAAATCAAGATCTAGTAG+AGG + chr5.2:411238-411257 MS.gene40527:CDS 35.0%
GTAACAATTGTGGAATCATG+TGG - chr5.2:410602-410621 None:intergenic 35.0%
GTTCATTCCATTGTAAGCAA+TGG - chr5.2:412009-412028 None:intergenic 35.0%
TAATCGAGCTTGATTGAAAG+CGG - chr5.2:411485-411504 None:intergenic 35.0%
TACCCCAACAAAAGAAACAA+AGG + chr5.2:412049-412068 MS.gene40527:CDS 35.0%
TCATCAGATTGTGTTGTACA+TGG - chr5.2:410734-410753 None:intergenic 35.0%
TCTTGAGAAATAAGCTCAGT+AGG - chr5.2:410446-410465 None:intergenic 35.0%
TGACATTCCTCTTGTTCTTT+GGG - chr5.2:411042-411061 None:intergenic 35.0%
TGTTGTACATGGAGTGAATT+TGG - chr5.2:410723-410742 None:intergenic 35.0%
TTGACATTCCTCTTGTTCTT+TGG - chr5.2:411043-411062 None:intergenic 35.0%
TTTGAGCCAGAATGATTCTA+CGG + chr5.2:411204-411223 MS.gene40527:CDS 35.0%
! ACTGCTGCATTCTCTTTTAT+TGG - chr5.2:412092-412111 None:intergenic 35.0%
! ATGCAAGAGTAAGTCTTTTG+TGG + chr5.2:411287-411306 MS.gene40527:intron 35.0%
! CTGAGCTTATTTCTCAAGAA+TGG + chr5.2:410447-410466 MS.gene40527:CDS 35.0%
! GTACTTCACGTTCTGATTTT+CGG - chr5.2:410787-410806 None:intergenic 35.0%
! TACTTCACGTTCTGATTTTC+GGG - chr5.2:410786-410805 None:intergenic 35.0%
!! TTTTTGTTGATTAACCCTGC+AGG + chr5.2:411789-411808 MS.gene40527:intron 35.0%
AAGATCTAGGAGCTCAAATG+AGG + chr5.2:410859-410878 MS.gene40527:CDS 40.0%
AATCGAGCTTGATTGAAAGC+GGG - chr5.2:411484-411503 None:intergenic 40.0%
AATTCTTCTCATGAGCTACG+TGG + chr5.2:411166-411185 MS.gene40527:CDS 40.0%
ACAATTGTGGAATCATGTGG+TGG - chr5.2:410599-410618 None:intergenic 40.0%
ACCAGGACTTGTATTCTCTT+CGG - chr5.2:411101-411120 None:intergenic 40.0%
ACGTAGCTCATGAGAAGAAT+TGG - chr5.2:411167-411186 None:intergenic 40.0%
ATCCAAACCATAAACTGTGG+TGG - chr5.2:410977-410996 None:intergenic 40.0%
ATGAGGAGTTACCAAGTAAC+TGG - chr5.2:410520-410539 None:intergenic 40.0%
ATTCTGTTGCTGTCCATGTA+AGG + chr5.2:411596-411615 MS.gene40527:intron 40.0%
CATAGGCCAAATACATCTTG+TGG + chr5.2:411330-411349 MS.gene40527:intron 40.0%
CTTGTATCAGAAGTTGAGGT+AGG - chr5.2:410635-410654 None:intergenic 40.0%
GCAACTTGTATCAGAAGTTG+AGG - chr5.2:410639-410658 None:intergenic 40.0%
GTTAAGCGGTTATTGTTCAG+AGG + chr5.2:411135-411154 MS.gene40527:CDS 40.0%
TACTCTCCCATTGCTTACAA+TGG + chr5.2:411999-412018 MS.gene40527:CDS 40.0%
TATAGGTCCCAAAGAACAAG+AGG + chr5.2:411032-411051 MS.gene40527:intron 40.0%
TTCCACCACAGTTTATGGTT+TGG + chr5.2:410972-410991 MS.gene40527:intron 40.0%
! ACTAGTTTCTGAACCAGAAG+AGG + chr5.2:410805-410824 MS.gene40527:CDS 40.0%
! TCGTTTTCCACCACAGTTTA+TGG + chr5.2:410967-410986 MS.gene40527:intron 40.0%
! TTCTAAGCTCTGATGACATG+TGG + chr5.2:411973-411992 MS.gene40527:CDS 40.0%
!!! CTTGTGGTTTTATCCTCTTC+TGG - chr5.2:410821-410840 None:intergenic 40.0%
!!! TGGGTACTGAAACTGCATTT+TGG + chr5.2:410573-410592 MS.gene40527:CDS 40.0%
ACCACAAGCGCAGAAAATTC+AGG + chr5.2:410833-410852 MS.gene40527:CDS 45.0%
ACCGAAGAGAATACAAGTCC+TGG + chr5.2:411097-411116 MS.gene40527:CDS 45.0%
CTACAAAACCTTGTCCCCAA+TGG + chr5.2:411685-411704 MS.gene40527:CDS 45.0%
TCTCAGGATCAGACGTTAAG+CGG + chr5.2:411121-411140 MS.gene40527:CDS 45.0%
TTCTCATGAGCTACGTGGAA+GGG + chr5.2:411171-411190 MS.gene40527:CDS 45.0%
! CAAGAATGGACTTCTCTCAG+TGG + chr5.2:410461-410480 MS.gene40527:CDS 45.0%
! GAATACAAGTCCTGGTTCTC+AGG + chr5.2:411105-411124 MS.gene40527:CDS 45.0%
! TCCTGAATTTTCTGCGCTTG+TGG - chr5.2:410837-410856 None:intergenic 45.0%
! TTGTTGATTAACCCTGCAGG+TGG + chr5.2:411792-411811 MS.gene40527:intron 45.0%
!! CATTGGGGACAAGGTTTTGT+AGG - chr5.2:411687-411706 None:intergenic 45.0%
AACGTCTGATCCTGAGAACC+AGG - chr5.2:411118-411137 None:intergenic 50.0%
ACTCTTGCATAGAGGCTCTG+TGG - chr5.2:411277-411296 None:intergenic 50.0%
CCTTAGTTCCATTGGGGACA+AGG - chr5.2:411696-411715 None:intergenic 50.0%
CCTTGTCCCCAATGGAACTA+AGG + chr5.2:411693-411712 MS.gene40527:CDS 50.0%
CTTCTCATGAGCTACGTGGA+AGG + chr5.2:411170-411189 MS.gene40527:CDS 50.0%
GGATATCAGCACCATGCTTG+AGG + chr5.2:411813-411832 MS.gene40527:CDS 50.0%
TTGGACAGCTTCCTCAAGCA+TGG - chr5.2:411827-411846 None:intergenic 50.0%
AGGCTCTGTGGATCAGTAGC+AGG - chr5.2:411265-411284 None:intergenic 55.0%
! CAGTGGACTCTACACTGCTG+AGG + chr5.2:410478-410497 MS.gene40527:CDS 55.0%
!! GGTGCTGATATCCACCTGCA+GGG - chr5.2:411806-411825 None:intergenic 55.0%
!! TGGTGCTGATATCCACCTGC+AGG - chr5.2:411807-411826 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 410437 412115 410437 ID=MS.gene40527
chr5.2 mRNA 410437 412115 410437 ID=MS.gene40527.t1;Parent=MS.gene40527
chr5.2 exon 410437 410880 410437 ID=MS.gene40527.t1.exon1;Parent=MS.gene40527.t1
chr5.2 CDS 410437 410880 410437 ID=cds.MS.gene40527.t1;Parent=MS.gene40527.t1
chr5.2 exon 411037 411294 411037 ID=MS.gene40527.t1.exon2;Parent=MS.gene40527.t1
chr5.2 CDS 411037 411294 411037 ID=cds.MS.gene40527.t1;Parent=MS.gene40527.t1
chr5.2 exon 411649 411714 411649 ID=MS.gene40527.t1.exon3;Parent=MS.gene40527.t1
chr5.2 CDS 411649 411714 411649 ID=cds.MS.gene40527.t1;Parent=MS.gene40527.t1
chr5.2 exon 411811 411876 411811 ID=MS.gene40527.t1.exon4;Parent=MS.gene40527.t1
chr5.2 CDS 411811 411876 411811 ID=cds.MS.gene40527.t1;Parent=MS.gene40527.t1
chr5.2 exon 411972 412115 411972 ID=MS.gene40527.t1.exon5;Parent=MS.gene40527.t1
chr5.2 CDS 411972 412115 411972 ID=cds.MS.gene40527.t1;Parent=MS.gene40527.t1
Gene Sequence

>MS.gene40527

ATGGAACCTACTGAGCTTATTTCTCAAGAATGGACTTCTCTCAGTGGACTCTACACTGCTGAGGAATCTGACTTTATGAACCAGTTACTTGGTAACTCCTCATTCTCACAACATTTCTATCAAAATTCCAATTTGTTGGGTACTGAAACTGCATTTTGGCCACCACATGATTCCACAATTGTTACTGCACAAGTTCCTACCTCAACTTCTGATACAAGTTGCTTCAACAACAATCCAGTAACCAGCTTTGACTATATTTCTATGGGTCTTTCCATAGATCATTCCAAATTCACTCCATGTACAACACAATCTGATGAACAAGTTCTAGCTAACAAAAATTTGCAGGCCCGAAAATCAGAACGTGAAGTACTAGTTTCTGAACCAGAAGAGGATAAAACCACAAGCGCAGAAAATTCAGGAAAAAGATCTAGGAGCTCAAATGAGGTAACAAACAGAAGGCCCATGAATTAATCTTCTAAATGTTTCCCTTACTACTGTTTTTTATTTCTTCTTTCCAAGTGATATGCATTTCGTTTTCCACCACAGTTTATGGTTTGGATTTTGATTTTCTGAAATTCTCCGTGTCATTTGATTTTATAGGTCCCAAAGAACAAGAGGAATGTCAAATGTAGGAAGATTATAAGATGTGCTTCTATAAGCACCGAAGAGAATACAAGTCCTGGTTCTCAGGATCAGACGTTAAGCGGTTATTGTTCAGAGGATGAGTCCAATTCTTCTCATGAGCTACGTGGAAGGGAATCTTCAAGTTTGAGCCAGAATGATTCTACGGCTAAATTAAGTGGAAAATCAAGATCTAGTAGAGGTCCTGCTACTGATCCACAGAGCCTCTATGCAAGAGTAAGTCTTTTGTGGAATAGCAACACTTAGTTTCTCATAGGCCAAATACATCTTGTGGTCCTATTTAATGTTTGTATATCGAATCGAACTAATCCTTCTAGTTTTCTAATTTCAAATTGATCATTTATGTTAGTTAAAATGAACTTATTCCGTAAGTCATAAAAGATATGCACTATTAGTCTATTACCCGCTTTCAATCAAGCTCGATTAAAAGGTGTTTATGTGGATGTTAATTGTTAAGTTGGTAGGTTCTAAAAAGTGCTTATTCATAATGTATTTGGTCTTTCTAATAATATATGATATTCTGTTGCTGTCCATGTAAGGTCTTACCATGTTTCCTTTTGTATTGTATAGAAAAGAAGAGAAAGAATAAATGAAAGGTTGAAAATCCTACAAAACCTTGTCCCCAATGGAACTAAGGTAACTTTCTTTCTCAATGCCATTCAGACTATATATTGAACAACAATTGGTTCATCTGCTAAAAATTCTTTTATTTTTTGTTGATTAACCCTGCAGGTGGATATCAGCACCATGCTTGAGGAAGCTGTCCAATATGTGAAGTTTTTACAACTTCAAATTAAGGTTTGAGGATTCTCCTTTCTCTCTCTGCATTTAAAATTTGAGCTCAATGCAAAGACAATTACGTACTGATCCCTATATGAATTTGAATGGAACAGCTTCTAAGCTCTGATGACATGTGGATGTACTCTCCCATTGCTTACAATGGAATGAACATTGGACTTGAACTTGGTATTACCCCAACAAAAGAAACAAAGGATATGCATAACAACATACCAATAAAAGAGAATGCAGCAGTTTAA
Protein sequence

>MS.gene40527.t1

MEPTELISQEWTSLSGLYTAEESDFMNQLLGNSSFSQHFYQNSNLLGTETAFWPPHDSTIVTAQVPTSTSDTSCFNNNPVTSFDYISMGLSIDHSKFTPCTTQSDEQVLANKNLQARKSEREVLVSEPEEDKTTSAENSGKRSRSSNEVPKNKRNVKCRKIIRCASISTEENTSPGSQDQTLSGYCSEDESNSSHELRGRESSSLSQNDSTAKLSGKSRSSRGPATDPQSLYARKRRERINERLKILQNLVPNGTKVDISTMLEEAVQYVKFLQLQIKLLSSDDMWMYSPIAYNGMNIGLELGITPTKETKDMHNNIPIKENAAV