Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40553.t1 | XP_003602464.1 | 99 | 103 | 1 | 0 | 1 | 103 | 30 | 132 | 1.10E-51 | 212.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40553.t1 | Q8L5T5 | 95.0 | 101 | 5 | 0 | 3 | 103 | 30 | 130 | 4.1e-50 | 198.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40553.t1 | G7J9S3 | 99.0 | 103 | 1 | 0 | 1 | 103 | 30 | 132 | 8.3e-52 | 212.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene40553.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40553.t1 | MTR_3g094690 | 99.029 | 103 | 1 | 0 | 1 | 103 | 30 | 132 | 7.38e-73 | 216 |
| MS.gene40553.t1 | MTR_3g094690 | 98.058 | 103 | 1 | 1 | 1 | 103 | 30 | 131 | 3.16e-70 | 209 |
| MS.gene40553.t1 | MTR_5g083960 | 91.837 | 98 | 8 | 0 | 6 | 103 | 27 | 124 | 1.66e-64 | 194 |
| MS.gene40553.t1 | MTR_5g075020 | 82.828 | 99 | 15 | 1 | 1 | 97 | 24 | 122 | 8.67e-59 | 180 |
| MS.gene40553.t1 | MTR_5g075020 | 82.828 | 99 | 15 | 1 | 1 | 97 | 28 | 126 | 1.45e-58 | 180 |
| MS.gene40553.t1 | MTR_5g083230 | 74.157 | 89 | 23 | 0 | 17 | 105 | 5 | 93 | 7.42e-45 | 143 |
| MS.gene40553.t1 | MTR_3g071590 | 70.652 | 92 | 27 | 0 | 14 | 105 | 4 | 95 | 4.22e-44 | 140 |
| MS.gene40553.t1 | MTR_4g083680 | 72.414 | 87 | 24 | 0 | 17 | 103 | 14 | 100 | 5.13e-44 | 141 |
| MS.gene40553.t1 | MTR_2g068760 | 70.787 | 89 | 26 | 0 | 15 | 103 | 10 | 98 | 7.53e-44 | 140 |
| MS.gene40553.t1 | MTR_7g033800 | 67.816 | 87 | 28 | 0 | 17 | 103 | 10 | 96 | 1.90e-40 | 132 |
| MS.gene40553.t1 | MTR_6g005070 | 66.667 | 87 | 29 | 0 | 17 | 103 | 8 | 94 | 1.90e-40 | 131 |
| MS.gene40553.t1 | MTR_3g031660 | 67.416 | 89 | 29 | 0 | 17 | 105 | 6 | 94 | 2.14e-40 | 132 |
| MS.gene40553.t1 | MTR_6g005080 | 64.368 | 87 | 31 | 0 | 17 | 103 | 9 | 95 | 7.56e-39 | 127 |
| MS.gene40553.t1 | MTR_4g105170 | 66.667 | 87 | 29 | 0 | 17 | 103 | 10 | 96 | 8.40e-39 | 128 |
| MS.gene40553.t1 | MTR_3g077240 | 66.667 | 87 | 29 | 0 | 17 | 103 | 9 | 95 | 1.48e-38 | 127 |
| MS.gene40553.t1 | MTR_2g093310 | 62.500 | 88 | 33 | 0 | 16 | 103 | 31 | 118 | 4.76e-38 | 126 |
| MS.gene40553.t1 | MTR_3g073690 | 65.517 | 87 | 30 | 0 | 17 | 103 | 8 | 94 | 1.37e-37 | 125 |
| MS.gene40553.t1 | MTR_3g452660 | 63.218 | 87 | 32 | 0 | 17 | 103 | 6 | 92 | 4.36e-37 | 127 |
| MS.gene40553.t1 | MTR_5g017950 | 65.517 | 87 | 30 | 0 | 17 | 103 | 7 | 93 | 5.34e-37 | 127 |
| MS.gene40553.t1 | MTR_3g071420 | 65.882 | 85 | 29 | 0 | 19 | 103 | 15 | 99 | 7.47e-37 | 123 |
| MS.gene40553.t1 | MTR_8g040900 | 64.634 | 82 | 29 | 0 | 17 | 98 | 6 | 87 | 1.62e-36 | 123 |
| MS.gene40553.t1 | MTR_5g083010 | 64.706 | 85 | 30 | 0 | 19 | 103 | 12 | 96 | 9.10e-36 | 120 |
| MS.gene40553.t1 | MTR_1g095850 | 62.069 | 87 | 33 | 0 | 19 | 105 | 73 | 159 | 2.40e-35 | 120 |
| MS.gene40553.t1 | MTR_4g060950 | 60.674 | 89 | 34 | 1 | 16 | 103 | 22 | 110 | 5.52e-35 | 118 |
| MS.gene40553.t1 | MTR_7g074990 | 64.368 | 87 | 30 | 1 | 18 | 103 | 20 | 106 | 9.08e-34 | 117 |
| MS.gene40553.t1 | MTR_7g096530 | 55.914 | 93 | 40 | 1 | 15 | 106 | 11 | 103 | 3.18e-32 | 112 |
| MS.gene40553.t1 | MTR_7g028905 | 57.647 | 85 | 36 | 0 | 19 | 103 | 6 | 90 | 5.86e-32 | 108 |
| MS.gene40553.t1 | MTR_1g070220 | 57.471 | 87 | 36 | 1 | 18 | 103 | 15 | 101 | 3.78e-30 | 107 |
| MS.gene40553.t1 | MTR_5g093010 | 56.471 | 85 | 37 | 0 | 19 | 103 | 6 | 90 | 4.44e-30 | 104 |
| MS.gene40553.t1 | MTR_6g027710 | 58.427 | 89 | 36 | 1 | 16 | 103 | 38 | 126 | 5.52e-30 | 107 |
| MS.gene40553.t1 | MTR_1g070205 | 56.818 | 88 | 35 | 2 | 18 | 103 | 12 | 98 | 1.25e-28 | 103 |
| MS.gene40553.t1 | MTR_8g036105 | 58.621 | 87 | 35 | 1 | 18 | 103 | 18 | 104 | 1.53e-28 | 103 |
| MS.gene40553.t1 | MTR_7g096610 | 55.056 | 89 | 37 | 2 | 17 | 103 | 7 | 94 | 6.61e-28 | 101 |
| MS.gene40553.t1 | MTR_8g036085 | 55.172 | 87 | 38 | 1 | 18 | 103 | 23 | 109 | 1.38e-27 | 100 |
| MS.gene40553.t1 | MTR_8g079660 | 53.409 | 88 | 41 | 0 | 16 | 103 | 6 | 93 | 1.45e-27 | 101 |
| MS.gene40553.t1 | MTR_5g080470 | 51.136 | 88 | 42 | 1 | 17 | 103 | 6 | 93 | 2.74e-27 | 99.4 |
| MS.gene40553.t1 | MTR_6g011250 | 45.000 | 80 | 43 | 1 | 19 | 97 | 10 | 89 | 1.25e-24 | 92.0 |
| MS.gene40553.t1 | MTR_7g075230 | 52.809 | 89 | 39 | 2 | 18 | 103 | 12 | 100 | 1.53e-23 | 89.7 |
| MS.gene40553.t1 | MTR_8g079620 | 47.674 | 86 | 45 | 0 | 16 | 101 | 6 | 91 | 1.80e-23 | 90.5 |
| MS.gene40553.t1 | MTR_8g079580 | 52.273 | 88 | 41 | 1 | 16 | 102 | 6 | 93 | 3.30e-23 | 89.4 |
| MS.gene40553.t1 | MTR_6g027700 | 45.977 | 87 | 46 | 1 | 18 | 103 | 7 | 93 | 8.55e-23 | 88.2 |
| MS.gene40553.t1 | MTR_6g011230 | 42.857 | 84 | 48 | 0 | 14 | 97 | 7 | 90 | 1.17e-22 | 87.4 |
| MS.gene40553.t1 | MTR_6g011200 | 41.573 | 89 | 48 | 2 | 13 | 97 | 1 | 89 | 1.01e-21 | 86.3 |
| MS.gene40553.t1 | MTR_4g107450 | 43.820 | 89 | 50 | 0 | 15 | 103 | 7 | 95 | 1.15e-20 | 84.0 |
| MS.gene40553.t1 | MTR_5g085390 | 40.449 | 89 | 49 | 2 | 13 | 97 | 1 | 89 | 1.86e-19 | 79.7 |
| MS.gene40553.t1 | MTR_4g099230 | 41.250 | 80 | 46 | 1 | 19 | 97 | 3 | 82 | 1.07e-18 | 76.3 |
| MS.gene40553.t1 | MTR_8g017090 | 40.000 | 90 | 52 | 2 | 15 | 103 | 9 | 97 | 6.60e-18 | 76.6 |
| MS.gene40553.t1 | MTR_2g100020 | 40.000 | 90 | 52 | 2 | 15 | 103 | 16 | 104 | 4.43e-17 | 74.3 |
| MS.gene40553.t1 | MTR_4g088035 | 34.483 | 87 | 57 | 0 | 17 | 103 | 5 | 91 | 2.13e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40553.t1 | AT2G40470 | 94.792 | 96 | 5 | 0 | 3 | 98 | 5 | 100 | 2.65e-63 | 191 |
| MS.gene40553.t1 | AT2G40470 | 94.792 | 96 | 5 | 0 | 3 | 98 | 30 | 125 | 3.86e-63 | 191 |
| MS.gene40553.t1 | AT2G30340 | 83.810 | 105 | 15 | 2 | 1 | 103 | 33 | 137 | 9.26e-61 | 186 |
| MS.gene40553.t1 | AT2G30340 | 83.810 | 105 | 15 | 2 | 1 | 103 | 34 | 138 | 1.05e-60 | 186 |
| MS.gene40553.t1 | AT1G31320 | 73.563 | 87 | 23 | 0 | 17 | 103 | 12 | 98 | 2.16e-43 | 139 |
| MS.gene40553.t1 | AT2G30130 | 67.677 | 99 | 28 | 1 | 7 | 105 | 1 | 95 | 2.19e-43 | 140 |
| MS.gene40553.t1 | AT3G27650 | 62.626 | 99 | 37 | 0 | 5 | 103 | 26 | 124 | 2.17e-41 | 134 |
| MS.gene40553.t1 | AT5G66870 | 68.966 | 87 | 27 | 0 | 17 | 103 | 6 | 92 | 1.90e-39 | 133 |
| MS.gene40553.t1 | AT1G16530 | 62.222 | 90 | 33 | 1 | 15 | 103 | 11 | 100 | 4.47e-39 | 128 |
| MS.gene40553.t1 | AT1G65620 | 68.235 | 85 | 27 | 0 | 14 | 98 | 5 | 89 | 8.90e-39 | 128 |
| MS.gene40553.t1 | AT1G65620 | 68.235 | 85 | 27 | 0 | 14 | 98 | 5 | 89 | 8.90e-39 | 128 |
| MS.gene40553.t1 | AT1G65620 | 68.235 | 85 | 27 | 0 | 14 | 98 | 5 | 89 | 8.90e-39 | 128 |
| MS.gene40553.t1 | AT1G65620 | 68.235 | 85 | 27 | 0 | 14 | 98 | 5 | 89 | 8.90e-39 | 128 |
| MS.gene40553.t1 | AT1G65620 | 68.235 | 85 | 27 | 0 | 14 | 98 | 5 | 89 | 8.90e-39 | 128 |
| MS.gene40553.t1 | AT5G63090 | 65.517 | 87 | 30 | 0 | 17 | 103 | 10 | 96 | 4.26e-38 | 126 |
| MS.gene40553.t1 | AT5G63090 | 65.517 | 87 | 30 | 0 | 17 | 103 | 10 | 96 | 4.26e-38 | 126 |
| MS.gene40553.t1 | AT5G63090 | 65.517 | 87 | 30 | 0 | 17 | 103 | 10 | 96 | 4.26e-38 | 126 |
| MS.gene40553.t1 | AT5G63090 | 65.517 | 87 | 30 | 0 | 17 | 103 | 10 | 96 | 4.26e-38 | 126 |
| MS.gene40553.t1 | AT1G07900 | 61.538 | 91 | 35 | 0 | 15 | 105 | 30 | 120 | 4.86e-38 | 126 |
| MS.gene40553.t1 | AT2G28500 | 62.222 | 90 | 34 | 0 | 16 | 105 | 53 | 142 | 1.68e-37 | 126 |
| MS.gene40553.t1 | AT2G23660 | 65.116 | 86 | 30 | 0 | 17 | 102 | 4 | 89 | 2.54e-37 | 128 |
| MS.gene40553.t1 | AT2G23660 | 65.116 | 86 | 30 | 0 | 17 | 102 | 4 | 89 | 2.54e-37 | 128 |
| MS.gene40553.t1 | AT2G23660 | 65.116 | 86 | 30 | 0 | 17 | 102 | 4 | 89 | 2.54e-37 | 128 |
| MS.gene40553.t1 | AT3G11090 | 63.529 | 85 | 31 | 0 | 19 | 103 | 12 | 96 | 1.20e-34 | 117 |
| MS.gene40553.t1 | AT3G26620 | 58.824 | 85 | 35 | 0 | 19 | 103 | 6 | 90 | 2.53e-34 | 115 |
| MS.gene40553.t1 | AT3G26660 | 58.824 | 85 | 35 | 0 | 19 | 103 | 6 | 90 | 2.64e-34 | 115 |
| MS.gene40553.t1 | AT2G42430 | 54.639 | 97 | 43 | 1 | 8 | 103 | 5 | 101 | 2.45e-31 | 110 |
| MS.gene40553.t1 | AT4G00210 | 59.770 | 87 | 34 | 1 | 18 | 103 | 11 | 97 | 1.28e-30 | 108 |
| MS.gene40553.t1 | AT3G58190 | 56.818 | 88 | 35 | 2 | 18 | 103 | 11 | 97 | 2.24e-28 | 102 |
| MS.gene40553.t1 | AT5G06080 | 55.682 | 88 | 38 | 1 | 17 | 103 | 6 | 93 | 2.66e-28 | 101 |
| MS.gene40553.t1 | AT3G03760 | 55.682 | 88 | 38 | 1 | 17 | 103 | 50 | 137 | 5.71e-28 | 102 |
| MS.gene40553.t1 | AT2G31310 | 50.000 | 88 | 43 | 1 | 17 | 103 | 6 | 93 | 1.78e-27 | 99.8 |
| MS.gene40553.t1 | AT4G00220 | 57.143 | 84 | 35 | 1 | 21 | 103 | 20 | 103 | 3.48e-27 | 99.8 |
| MS.gene40553.t1 | AT2G45420 | 54.762 | 84 | 37 | 1 | 21 | 103 | 40 | 123 | 1.37e-26 | 99.0 |
| MS.gene40553.t1 | AT2G42440 | 51.685 | 89 | 40 | 2 | 17 | 103 | 6 | 93 | 3.17e-26 | 97.8 |
| MS.gene40553.t1 | AT2G45410 | 53.571 | 84 | 38 | 1 | 21 | 103 | 19 | 102 | 2.90e-25 | 94.0 |
| MS.gene40553.t1 | AT3G47870 | 43.373 | 83 | 47 | 0 | 19 | 101 | 37 | 119 | 9.36e-23 | 90.1 |
| MS.gene40553.t1 | AT3G50510 | 45.977 | 87 | 47 | 0 | 17 | 103 | 11 | 97 | 1.39e-22 | 87.4 |
| MS.gene40553.t1 | AT3G50510 | 45.977 | 87 | 47 | 0 | 17 | 103 | 11 | 97 | 1.39e-22 | 87.4 |
| MS.gene40553.t1 | AT1G06280 | 48.052 | 77 | 40 | 0 | 19 | 95 | 25 | 101 | 9.72e-21 | 82.8 |
| MS.gene40553.t1 | AT1G72980 | 42.529 | 87 | 50 | 0 | 15 | 101 | 10 | 96 | 3.93e-19 | 78.6 |
| MS.gene40553.t1 | AT3G13850 | 38.824 | 85 | 52 | 0 | 19 | 103 | 37 | 121 | 1.71e-18 | 77.8 |
| MS.gene40553.t1 | AT5G35900 | 39.080 | 87 | 53 | 0 | 17 | 103 | 4 | 90 | 5.32e-18 | 75.5 |
| MS.gene40553.t1 | AT2G19820 | 34.483 | 87 | 56 | 1 | 18 | 103 | 12 | 98 | 2.13e-15 | 67.0 |
| MS.gene40553.t1 | AT5G15060 | 35.526 | 76 | 47 | 1 | 16 | 89 | 15 | 90 | 1.30e-11 | 57.4 |
| MS.gene40553.t1 | AT1G36000 | 31.034 | 87 | 58 | 2 | 18 | 103 | 9 | 94 | 6.80e-11 | 55.5 |
Find 0 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACTTAAATACAAATTA+AGG | + | 22534:9521-9540 | MS.gene40553:intron | 10.0% |
| !! | AATACTTAAATACAAATTAA+GGG | + | 22534:9522-9541 | MS.gene40553:intron | 10.0% |
| !!! | AATATATATAATGTTTTAGG+AGG | + | 22534:9345-9364 | MS.gene40553:intron | 15.0% |
| ACAAGGGGTTATAGTATTTA+GGG | - | 22534:9123-9142 | MS.gene40553:intergenic | 30.0% | |
| TTGTTTATGAAGCAAATGTG+AGG | + | 22534:9404-9423 | MS.gene40553:CDS | 30.0% | |
| AATTTCTGCATTGCAACAAC+AGG | + | 22534:9459-9478 | MS.gene40553:CDS | 35.0% | |
| ATGGGAAGAAGACATATGTT+AGG | + | 22534:9086-9105 | MS.gene40553:CDS | 35.0% | |
| CAACGTCTCAAAAATGCTAA+TGG | + | 22534:9256-9275 | MS.gene40553:CDS | 35.0% | |
| CACAAGGGGTTATAGTATTT+AGG | - | 22534:9124-9143 | MS.gene40553:intergenic | 35.0% | |
| GCAGAACTCAATCTATTAAG+AGG | + | 22534:9496-9515 | MS.gene40553:intron | 35.0% | |
| ! | GCTTCTGTTCACAAAGTTTT+TGG | + | 22534:9227-9246 | MS.gene40553:CDS | 35.0% |
| ! | GTTATAGTATTTAGGGTTCC+TGG | - | 22534:9116-9135 | MS.gene40553:intergenic | 35.0% |
| AATAGCTTACATGCTGCACA+AGG | - | 22534:9140-9159 | MS.gene40552:intergenic | 40.0% | |
| AGATCCAGTTTATGGATGCA+TGG | + | 22534:9432-9451 | MS.gene40553:CDS | 40.0% | |
| AGGAGAGAAATAGGGAGAAA+AGG | - | 22534:9189-9208 | MS.gene40553:intergenic | 40.0% | |
| AGGCTAAGAGATCCAGTTTA+TGG | + | 22534:9424-9443 | MS.gene40553:CDS | 40.0% | |
| ATAGCTTACATGCTGCACAA+GGG | - | 22534:9139-9158 | MS.gene40553:intergenic | 40.0% | |
| GATCCAGTTTATGGATGCAT+GGG | + | 22534:9433-9452 | MS.gene40553:CDS | 40.0% | |
| GGAGAGAAATAGGGAGAAAA+GGG | - | 22534:9188-9207 | MS.gene40553:intergenic | 40.0% | |
| GGTTCATGAGGAGAGAAATA+GGG | - | 22534:9197-9216 | MS.gene40553:intergenic | 40.0% | |
| TGAACAGAAGCAAACTTCTG+TGG | - | 22534:9218-9237 | MS.gene40553:intergenic | 40.0% | |
| TGGTTCATGAGGAGAGAAAT+AGG | - | 22534:9198-9217 | MS.gene40553:intergenic | 40.0% | |
| ! | ATAGTATTTAGGGTTCCTGG+TGG | - | 22534:9113-9132 | MS.gene40553:intergenic | 40.0% |
| !!! | TAGCATTTTTGAGACGTTGC+TGG | - | 22534:9255-9274 | MS.gene40553:intergenic | 40.0% |
| AGACATATGTTAGGTCCACC+AGG | + | 22534:9095-9114 | MS.gene40553:CDS | 45.0% | |
| GCAAACTTCTGTGGTTCATG+AGG | - | 22534:9209-9228 | MS.gene40553:intergenic | 45.0% | |
| TAGCTTACATGCTGCACAAG+GGG | - | 22534:9138-9157 | MS.gene40553:intergenic | 45.0% | |
| !!! | AATAATATATATAATGTTTT+AGG | + | 22534:9342-9361 | MS.gene40553:intron | 5.0% |
| GCACCCATGCATCCATAAAC+TGG | - | 22534:9439-9458 | MS.gene40553:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 22534 | gene | 9086 | 9554 | 9086 | ID=MS.gene40553 |
| 22534 | mRNA | 9086 | 9554 | 9086 | ID=MS.gene40553.t1;Parent=MS.gene40553 |
| 22534 | exon | 9086 | 9277 | 9086 | ID=MS.gene40553.t1.exon1;Parent=MS.gene40553.t1 |
| 22534 | CDS | 9086 | 9277 | 9086 | ID=cds.MS.gene40553.t1;Parent=MS.gene40553.t1 |
| 22534 | exon | 9364 | 9480 | 9364 | ID=MS.gene40553.t1.exon2;Parent=MS.gene40553.t1 |
| 22534 | CDS | 9364 | 9480 | 9364 | ID=cds.MS.gene40553.t1;Parent=MS.gene40553.t1 |
| 22534 | exon | 9543 | 9554 | 9543 | ID=MS.gene40553.t1.exon3;Parent=MS.gene40553.t1 |
| 22534 | CDS | 9543 | 9554 | 9543 | ID=cds.MS.gene40553.t1;Parent=MS.gene40553.t1 |
| Gene Sequence |
| Protein sequence |
